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    Sample Lab Report - DNA Extraction From Cheek Cells

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    JV Gamo
    This document describes a procedure to extract DNA from human cheek cells. The procedure involved collecting cheek cells by rinsing the mouth with water, then lysing the cells and digesting proteins to isolate the DNA. Specifically, lysis buffer was used to break open the cells, protease to break down proteins, and ethanol to precipitate the DNA. Following this protocol, DNA was successfully extracted from the cheek cells, visible as a white precipitate, and can now be purified for use in other experiments such as sequencing or PCR analysis.

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    Sample Lab Report - DNA Extraction From Cheek Cells

    Uploaded by

    JV Gamo
    3K views2 pages
    This document describes a procedure to extract DNA from human cheek cells. The procedure involved collecting cheek cells by rinsing the mouth with water, then lysing the cells and digesting proteins to isolate the DNA. Specifically, lysis buffer was used to break open the cells, protease to break down proteins, and ethanol to precipitate the DNA. Following this protocol, DNA was successfully extracted from the cheek cells, visible as a white precipitate, and can now be purified for use in other experiments such as sequencing or PCR analysis.

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    Sample Lab Report- DNA Extraction From Cheek Cells

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    This document describes a procedure to extract DNA from human cheek cells. The procedure involved collecting cheek cells by rinsing the mouth with water, then lysing the cells and digesting proteins to isolate the DNA. Specifically, lysis buffer was used to break open the cells, protease to break down proteins, and ethanol to precipitate the DNA. Following this protocol, DNA was successfully extracted from the cheek cells, visible as a white precipitate, and can now be purified for use in other experiments such as sequencing or PCR analysis.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    3K views2 pages

    Sample Lab Report - DNA Extraction From Cheek Cells

    Uploaded by

    JV Gamo
    This document describes a procedure to extract DNA from human cheek cells. The procedure involved collecting cheek cells by rinsing the mouth with water, then lysing the cells and digesting proteins to isolate the DNA. Specifically, lysis buffer was used to break open the cells, protease to break down proteins, and ethanol to precipitate the DNA. Following this protocol, DNA was successfully extracted from the cheek cells, visible as a white precipitate, and can now be purified for use in other experiments such as sequencing or PCR analysis.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 2

    Extraction of DNA from Cheek Cells

    Gene Smith
    February 29th 2013
    INTRODUCTION
    DNA, deoxyribonucleic acid, is the genetic material of every living organism
    and is found in the nucleus of eukaryotic cells. DNA is often called the
    blueprint for life because it contains the necessary information to carry out
    all the living processes of the cell (1). The purpose of this lab was to extract
    DNA from human cheek cells. The isolated DNA could be used in mapping or
    sequencing, PCR, crime scene investigation or other downstream
    applications (2).
    MATERIALS AND METHODS
    To extract DNA from cheek cells, 3 mL of water was added to a labeled, 15
    mL tube. The inner lining of the cheek was then chewed for approximately 30
    seconds. The 3 mL of water was used to rinse the mouth for 30 seconds,
    after which the water and the cheek cells were expelled back into the 15 mL
    tube. Using a plastic transfer pipet, 2 mL of lysis buffer was added to the
    tube containing the water with the cheek cells. The tube was then capped
    and gently inverted 5 times to lyse the cells.
    Five drops of protease and salt solution were added to the sample. The tube
    was capped and inverted 5 times. The sample was incubated in a water bath
    at 50C for 10 minutes. Following incubation, 10 mL of cold ethanol was
    slowly added while holding the tube at a 45 angle. The tube was placed
    upright at room temperature for five minutes, after which the sample was
    inverted 5 times. The DNA extraction protocol and all materials and reagents
    were provided by Bio-Rad (2).
    RESULTS

    Throughout the experiment, numerous observations were made. The water


    containing cheek cells was white or cloudy in appearance. Upon addition of
    the lysis buffer and protease, the solution became clear. A white precipitate
    was visible following the addition of cold ethanol.
    DISCUSSION
    Extraction of DNA from cells is a relatively straight-forward and simple
    process. It is routinely carried out in laboratories and has numerous
    downstream applications, including sequencing and PCR.
    Only a few reagents were used to extract DNA from cells: lysis buffer,
    protease and ethanol. Lysis buffer broke open the cheek cells to expose DNA
    and cellular proteins. This explains why the sample in this experiment
    became clear upon addition of the lysis buffer. Protease served to
    enzymatically break down the proteins in the sample. Maximum activity of
    the enzyme was achieved by incubating the sample at 50C. Finally, DNA
    became visible as a white precipitate after adding cold ethanol. Extraction of
    DNA was successful following this protocol. The DNA sample can now be
    purified for use in future experiments.

    REFERENCES
    1. Russell, Peter J. 2010, iGenetics: A Molecular Approach, San Francisco:
    Benjamin Cummings Publishers, 3rd Ed.
    2. www.bio-rad.com

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