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Experiment 1

The document summarizes key information about using a compound microscope: 1. A compound microscope has three main parts - the mechanical parts, illuminating parts, and magnifying parts. It differs from a simple microscope in that it has two sets of lenses (eyepiece and objective lenses) which allows for higher magnification. 2. The Abbe condenser focuses light onto the specimen while the iris diaphragm regulates the amount of light. 3. Microscopes must be carefully maintained because damage could lead to inaccurate observations or expensive repairs. Calibrating the micrometer eyepiece ensures accurate measurements between microscopes.

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Christian Roy C
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452 views3 pages

Experiment 1

The document summarizes key information about using a compound microscope: 1. A compound microscope has three main parts - the mechanical parts, illuminating parts, and magnifying parts. It differs from a simple microscope in that it has two sets of lenses (eyepiece and objective lenses) which allows for higher magnification. 2. The Abbe condenser focuses light onto the specimen while the iris diaphragm regulates the amount of light. 3. Microscopes must be carefully maintained because damage could lead to inaccurate observations or expensive repairs. Calibrating the micrometer eyepiece ensures accurate measurements between microscopes.

Uploaded by

Christian Roy C
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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EXPERIMENT # 1

The Microscope
I.

ANSWER TO QUESTIONS

A. Exercise 1.1 The Compound Microscope


1. What are the three main parts of a compound microscope?
The three main parts are the:
a. Mechanical Parts, which is the framework of the entire microscope
b. Illuminating Parts, used for controlling the light entering the microscope,
and;
c. Magnifying Part, for enlargement of objects being observed.
2. In what way does a compound microscope differ from a simple microscope?
The difference between the two microscopes can be established based on
several factors such as the number of lenses, the level of magnification, cost,
portability, and the range of its use.
In terms of the total lenses on each of the microscopes, the simple
microscope has only one lens compared to the compound which has two sets, the
eyepiece and the objective lenses. Because of this factor, the total magnification
for each microscopes vary with each other wherein the compound microscope
yields a higher level of magnification than the simple microscope.
In conclusion, the compound microscope has a wide range of use
compared to the simple microscope. Such uses includes the studying of the cells
structure, behavior, and components.
3. Distinguish the differences between the Abbe condenser and the iris diaphragm
functionally speaking?
The Abbe condenser is used to concentrate or focus the light coming from
the diaphragm to the objects found on the stage, which illuminates the specimen
with uniform intensity, while the iris diaphragm is used to regulate the amount
of light entering the microscope which passes through the condenser, and then
into the specimen.

4. Why is it important to take very good care of your microscope?

It is important to take good care of your microscope because. 1) You may


see things that are not there (mainly because of unnecessary cracks or dusts) or
miss things that are there (because of a damaged part) if you failed to comply the
safety use of it, and 2) Microscopes are expensive together with its individual
parts and a damage may obviously lead to spending of money for repairs or
replacement.
B. Exercise 1.3 Calibration of the Micrometer Eyepiece
1. What is the reason for calibrating the micrometer eyepiece?
Micrometer eyepiece calibration should be practiced to help ensure that
the microscope used will output accurate and valid information. An example for
this occurs when a replicate sample is to be assessed with different microscope
and still yield a result same with original.
2. What is meant by calibration?
According to the International Vocabulary of Metrology, calibration is the
process of finding a relationship between two quantities that are unknown (when
the measurable quantities are not given a particular value for the amount
considered or found a standard for the quantity). When one of quantity is
known, which is made or set with one device, another measurement is made as
similar way as possible with the first device using a second device. The
measurable quantities may differ in two devices which are equivalent.

C. Exercise 1.4 Magnification


1. Does the magnification based upon the length measurement coincide with the
magnification based on the width?
Yes, the magnification based on the length measurement would coincide
with the magnification based on the width.
2. What is wrong if these two magnifications do not coincide?
If the two magnifications does not coincide it means that one of the
lens is distorted thus changing the field of view for the observer.
3. What is meant by a proportional drawing?
Proportional drawing means that the object drawn on the paper has the
real

proportions what it has in real life it allow objects look real but just smaller.

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