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Re-Extraction & Crystalization Filtration Centrifuge Extraction

The document describes the downstream processing steps for penicillin: 1) Filtration is used to remove biomass from the fermentation broth through rotary vacuum filtration while cooling and acidifying the liquid. 2) Centrifugation extracts the penicillin through a tubular bowl centrifuge operating at 25°C for 180 hours at 1600g. 3) The extracted penicillin undergoes re-extraction and crystallization in an agitated vessel at 65-75°C and pH 5.5-7.0 for 10-16 hours to concentrate it to 200,000-250,000 units before drying.

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40 views1 page

Re-Extraction & Crystalization Filtration Centrifuge Extraction

The document describes the downstream processing steps for penicillin: 1) Filtration is used to remove biomass from the fermentation broth through rotary vacuum filtration while cooling and acidifying the liquid. 2) Centrifugation extracts the penicillin through a tubular bowl centrifuge operating at 25°C for 180 hours at 1600g. 3) The extracted penicillin undergoes re-extraction and crystallization in an agitated vessel at 65-75°C and pH 5.5-7.0 for 10-16 hours to concentrate it to 200,000-250,000 units before drying.

Uploaded by

faraaaalya
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOCX, PDF, TXT or read online on Scribd
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DOWNSTREAM PROCESSING OF PENICILLIN

FILTRATION

3 steps:
Removal
biomass
Cooling
acidification
Rotary vacuum
filter
T = 20-28C

CENTRIFUGE
EXTRACTION

Tubular bowl
T = 25C
180 hours
1 600 g

Optimum T = 2328C
(keep in dry
environment)
Stored in container

STORAGE

RE-EXTRACTION
&
CRYSTALIZATION

BASKET
CENTRIFUGATION

FLUID BED
DRYING

T = 50-60C
pH = 5.5-7.0
1-2 hours

Agitated vessel
T = 65-75C
pH = 5.5-7.0
Pressure = 30-40
mmHg
10-16 hours
Concentration =
200000-2500000

Rotary vacuum filter


To separate crystal
Large horizontal belt
filter
Collection & drying
the crystal
T = 65-75C
pH = 5.5-7.0

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