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M1076

Technical data-culture media composition.

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Aarde Stanovnik
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117 views2 pages

M1076

Technical data-culture media composition.

Uploaded by

Aarde Stanovnik
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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M1076

MIU Medium Base


MIU Medium Base is recommended for detection of motility, urease and indole production.

Composition**
Ingredients

Gms / Litre

Casein enzymic hydrolysate


Dextrose
Sodium chloride
Phenol red
Agar
Final pH ( at 25C)
**Formula adjusted, standardized to suit performance parameters

10.000
1.000
5.000
0.010
2.000
6.80.2

Directions
Suspend 18 grams in 950 ml distilled water. Heat to boiling to dissolve the medium completely. Dispense in 95 ml amounts
into flasks and sterilize by autoclaving at 15 lbs pressure (121C) for 15 minutes. Cool to about 50-55C and add aseptically
5 ml sterile 40% Urea solution (FD048) per 95 ml basal medium. Mix well and dispense into sterile test tubes. Allow to cool
in an upright position.

Principle And Interpretation


MIU Medium Base is formulated to detect motility, urease and indole production in single tube.
Casein enzymic hydrolysate provide amino acids and other nitrogenous substances. Sodium chloride maintains osmotic
equilibrium. Dextrose is fermentable carbohydrate. Phenol red is the pH indicator which turns pink- red in alkaline conditions.
The test cultures are stab-inoculated.
Motility and urease reactions are read before testing Indole production. Motile organisms show either diffused growth or
turbidity extending away from stab inoculation line while nonmotile organisms grow along the stabline. Organisms that utilize
urea, produce ammonia which makes the medium alkaline, showing pink-red colour by change in the phenol red indicator
(1). Indole is produced from tryptophan present in casein enzymic hydrolysate (2,3). The indole produced combines with the
aldehyde present in the Kovac's reagent to form a red complex.

Quality Control
Appearance
Light orange to light pink coloured homogeneous free flowing powder
Gelling
Semisolid, comparable with 0.2% Agar gel.
Colour and Clarity of prepared medium
Yellowish orange coloured clear to slightly opalescent gel is obtained in tubes as butts afteraddition of urea solution.
Reaction
Reaction of basal medium (1.8 gm suspended in 95 ml distilled water) at 25C. pH : 6.80.2
pH
6.60-7.00
Cultural Response
M1076: Cultural characteristics observed with added 40% Urea solution (FD048) after an incubation at 35-37C for 18 - 24
hours.
Organism
Growth
Indole
Motility
Urease activity
Cultural Response
Escherichia coli ATCC
luxuriant
Positive
Positive,
Negative
25922
reaction, red growth away reaction,no
ring at the
from stabline change
Please refer disclaimer Overleaf.

HiMedia Laboratories

Technical Data

Klebsiella pneumoniae
ATCC 13883

luxuriant

Proteus mirabilis ATCC


25933

luxuriant

Proteus vulgaris ATCC


13315

luxuriant

Salmonella typhimurium
ATCC 14028

luxuriant

interface of the
medium
Negative
reaction
no colour
development /
cloudy ring

causing
turbidity
Negative
Weakly
growth
positive
along the
stabline,surrounding
medium
remains clear
Negative
Positive,
Positive
reaction
growth away reaction, cerise
no colour
from stabline colour
development / causing
cloudy ring
turbidity
Positive
Positive,
Positive
reaction, red growth away reaction, cerise
ring at the
from stabline colour
interface of the causing
medium
turbidity
Negative
Positive,
Negative
reaction
growth away reaction,no
no colour
from stabline change
development / causing
cloudy ring
turbidity

Storage and Shelf Life


Store below 30C in tightly closed container and use freshly prepared medium . Use before expiry date on the label.

Reference
1. Rustigian and Stuart (1941) Proc. Soc. Exp. Biol. Med., 47:108.
2. McFaddin J.F. (1985) Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams and
Wilkins, Baltimore
3. Ewing (1986) Edwards and Ewings 'Identification of Enterobacteriaceae', 4th ed. Elsevier Science Publishing Co., Inc., New
York.
Revision : 2 / 2015

Disclaimer :
User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in
this and other related HiMedia publications. The information contained in this publication is based on our research and development
work and is to the best of our knowledge true and accurate. HiMedia Laboratories Pvt Ltd reserves the right to make changes to
specifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use but
for laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not
be considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.
HiMedia Laboratories Pvt. Ltd. A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147
1919 Email: [email protected]

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