Hydrogen Production by The Hyperthermophilic Eubacterium, Using Cellulose Pretreated by Ionic Liquid
Hydrogen Production by The Hyperthermophilic Eubacterium, Using Cellulose Pretreated by Ionic Liquid
Hydrogen Production by The Hyperthermophilic Eubacterium, Using Cellulose Pretreated by Ionic Liquid
(2008) 51615168
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Bioenergy Research Center, Korea Institute of Energy Research, Daejeon 305-343, South Korea
a r t i c l e i n
f o
a b s t r a c t
The application of thermophilic bacteria to the production of hydrogen (H 2) from a cellu-
Article history:
Received 13 May 2008
Accepted 26 May 2008
Available online 9 August 2008
losic
biomass
has
captured
attention
of
engineering
researchers.
Thermotoga
Keywords:
H2. Batch cultiva- tions were carried out to investigate the influence of the chemical
Hydrogen production
36.1% (v/v) with ethanol as an anti-solvent. This was clearly higher than that from the
N2 sparging
concentration in the head- space obtained from the ionic liquid-pretreated cellulose was
acid- and alkali-pretreated cellu- loses (2224%). Cultivation with cellulose pretreated with
the ionic liquid at a concentration of 10% combining with N 2 sparging
showed
the
maximum cumulative H2 yield of 1280 mL H 2/L culture. This value is approximately 10fold higher than that of raw cellulose (122 mL H2/L culture). The H2 yield obtained from
fermentation with ionic liquid-pretreated cellulose was 2.2 mol H 2/mol glucose
equivalents. The results showed that the pretreat- ment of cellulose using an ionic liquid
has considerable potential for improving the direct conversion of cellulosic substrates into
H2 by bacteria.
2008 International Association for Hydrogen Energy. Published by Elsevier Ltd. All
rights
reserved.
1.
Introduction
516
2
2.
2.1.
2.2.
5163
i n t e r n a t i o n a l j o u r n al of hy d r o g en e n e r g y 33
(2008) 51615168
H2 content (%)
a 30
H2 production by
T. neapolitana.
2.3.
30 min
60 min
90 min
25
120 min
20
15
Analytical procedure
10
20
3.
3.1.
15
10
5
0
Untreated
10
15
20
5164
3.2.
NaOH
H2SO4
Glucose
(g/L)
Glucose
(g/L)
0
0
0
0
0
0.4 T 0.024
0.6 T 0.029
4.9 T 0.242
5.9 T 0.304
5.3 T 0.256
0.0 T 0.000
0.1 T 0.003
0.4 T 0.002
0.6 T 0.003
1.0 T 0.004
0.2 T 0.009
0.8 T 0.046
2.5 T 0.130
3.7 T 0.189
5.0 T 0.244
1
5
10
15
20
a 40
35
30
25
20
3.3.
15
30
60
90
120
10
5
min
min
min
min
30
25
20
15
10
5
0
Untreated
5
20
10
15
Table 2 Comparison of hydrogen production with different kinds of chemical pretreatment methods
H2 content
(%, v/v
headspace)
Methods
Untreated cellulose
NaOH pretreatment
(15%, 80 C, 60 min,
neutralization)
H2SO4 pretreatment
(10%, 80 C, 120 min,
neutralization)
[C4mim]Cl pretreatment
(10%, 80 C, 6 h, EtOH)
without N2 sparging
[C4mim]Cl pretreatment
(10%, 80 C, 6 h, EtOH)
with N2 sparging
Cellulose
degradability
(%, w/w)
H2 yield
mL H2/L
culture
mol H2/mol
glucose equivalent
6.1 T 0.31
22.7 T 1.06
122.0 T 6.2
454.0 T 21.2
1.59 T 0.087
1.22 T 0.061
12.2 T 0.74
54.6 T 2.68
24.9 T 1.45
498.0 T 30.0
0.95 T 0.053
77.2 T 3.78
36.1 T 1.81
720.2 T 34.6
1.22 T 0.067
87.6 T 4.51
36.1 T 1.81
1280 T 58.0
2.2 T 0.10
88.9 T 4.75
100
H2 content
Cellulose utilization
80
30
60
Cellulos
utilization
(%)
1500
120
Cellulose utilization H2 production
H2 prodution rate
90
1000
60
40
H2
production
rate (mmol
H2
20
40
500
30
20
10
0
10
0
Untreated
15
0
EtOH
MeOH
Anti-solvents
Water
Fig. 4 Comparison of the effect of various antisolvents in the cellulose recovering step after
[C4mim]Cl pretreatment on the H2 production
under N2 sparging conditions.
160
140
120
Cel
growth
(mg
protein/L
culture)
100
2
80
60
40
0
Glucose Cell protein
0
5
10
20
15
20
25
30
35
3.4.
Cellulos
2000
1800
80
1600
1400
60
1200
40
1000
800
5
600
0
10
35
15
20
25
30
20
t
Fig. 5 Effect of concentration of [C4mim]Clpretreated cellulose on the H2 production by
Thermotoga neapolitana under N2 sparging
conditions.
4.
Conclusion
Acknowledgement
This Research was supported by the Hydrogen Energy R&D
Center, one of the 21st Century Frontier R&D Programs,
funded by the Ministry of Science and Technology of Korea.
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