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The document explores the microbiome of sandy coastal ecosystems as a potential source for biotechnologically relevant strains and biomarkers. Molecular techniques were used to analyze the DNA extracted from sandy sediments and tissues of key amphipod species inhabiting sandy beaches. Results identified a large taxonomic diversity with taxa involved in biodegradation, antibiotic production, and ammonia oxidation. Seasonal shifts and relationships between community composition and environmental variables were also detected.

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6 views1 page

tmp8509 TMP

The document explores the microbiome of sandy coastal ecosystems as a potential source for biotechnologically relevant strains and biomarkers. Molecular techniques were used to analyze the DNA extracted from sandy sediments and tissues of key amphipod species inhabiting sandy beaches. Results identified a large taxonomic diversity with taxa involved in biodegradation, antibiotic production, and ammonia oxidation. Seasonal shifts and relationships between community composition and environmental variables were also detected.

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Exploring the microbiome of sandy coastal ecosystems.

A new source for


biotechnologically-relevant strains and biomarkers
Abdelrhman F. A. Khaled1, Ciaramella Marta1, Confalone Marco1, Mengoni Alessio1,
Schintu Marco2, Ugolini Alberto1
1

: Department of Biology, University of Florence, Sesto Fiorentino, Italy, e-mail:


[email protected], http://www.dbe.unifi.it/dblage/
2
: Department of Health, Molecular and Clinical Medicine, University of Cagliari, Italy
Rsum :
Sandy beaches are world-wide diffused and are constantly subjected to biotic and abiotic
disturbances. Supalittoral zone of sandy beaches may also contain human pathogens, due to
human impact by recreational use of beaches, or urbanization. In spite of their importance as
ecological transition zone between land and sea, only very recently the microbial ecology of
sandy sediments, has stirred attention. Here, we describe the composition and the dynamics,
in relation to environmental variables, of bacteria communities present in sandy beaches
ecosystems at Marine Protected Areas of Sardinia (Italy). In particular, we have analyzed the
microbiome of i) amphipod species inhabiting the dump band of sandy beaches and, ii) the
sandy supralittoral sediments. Molecular techniques (T-RFLP, Real-Time PCR, NGS barcode
sequencing) were applied on DNA extracted from sandy sediments and from tissue of key
amphipod species of supralittoral habitats.
Obtained results indicated the presence of a very large taxonomic diversity in both the sandy
sediments and in animals, with the presence of taxa known to posses pathways for
biodegradation of cellulose and antibiotic production as well as ammonia oxidation.
Moreover, a large number of Actinomycetes and Alphaprotobacteria was found in the gut
tissues of amphipods, which could be related to cellulose degradation and probiotic
acetobacteria, similarly to recent findings obtained in Insects, as Anopheles spp. Finally,
seasonal shifts of bacterial communities were detected as well relationships between
community composition and environmental variables as geographical location and distance
from the dump band. However, additional work is required to better understand the influence
of anthropic pollution on the overall bacterial community.
Mots cls: communauts microbiennes, T-RFLP, cosystme costire, biomarker, amphipodes

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