Himedia BGLB Broth 2% M121

M121
Brilliant Green Bile Broth 2%
Brilliant Green Bile Broth 2% is recommended for the detection and confirmation of coliform bacteria in water, wastewater,
foods, milk and dairy products.
Composition**
Ingredients
Gms / Litre
Peptic digest of animal tissue

Lactose
Oxgall
Brilliant green
Final pH ( at 25C)
**Formula adjusted, standardized to suit performance parameters
10.000
10.000
20.000
0.0133
7.20.2
Directions
Suspend 40.01 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Distribute in fermentation
tubes containing inverted Durhams tubes and sterilize by autoclaving at 15 lbs pressure (121C) for 15 minutes.For preparation of
double strength it is recommended to heat the dissolved broth (80.02 grams per litre) at 100C for 30 minutes.
Principle And Interpretation

Brilliant Green Bile Broth 2% is one of the most widely used medium for the detection of coliform bacteria in water, wastewater,
foods, and milk and dairy products. This medium is formulated as per APHA (1, 2, 3) for the presumptive identification and
confirmation of coliform bacteria (4, 5). This medium is also recommended by the ISO Committee for enumeration of coliforms
by most probable number technique (6).
Peptic digest of animal tissue serves as a source of essential nutrients. Lactose is the fermentable carbohydrate. Ox gall inhibits
gram-positive bacteria whereas the gram-negative bacteria are inhibited by brilliant green. Production of gas from lactose
fermentation is detected by incorporating inverted Durham's tube, which indicates the positive evidence of faecal coliform
since non faecal coliforms growing in this medium do not produce gas. Further gas production in EC broth (M127) at 45C
used as a confirmation of faecal coliform. Gram-positive spore formers may produce gas if the bile or brilliant green inhibition
is weakened by reaction with food material.
During examination of water samples, growth from presumptive positive tubes showing gas in Lactose Broth (M026) or Lauryl
Tryptose Broth (M080) is inoculated in Brilliant Green Bile Broth 2% (M121). Gas formation within 48 2 hours confirms
the presumptive test (1).
Quality Control
Appearance
Cream to pale green homogeneous free flowing powder
Colour and Clarity of prepared medium
Emerald green coloured, clear solution without any precipitate.
Reaction
Reaction of 4.0% w/v aqueous solution at 25C. pH : 7.20.2
pH
7.00-7.40
Cultural Response
Cultural characteristics observed after an incubation at 35-37C for 18-48 hours.
Cultural Response
Organism
Inoculum
(CFU)
Cultural Response
Please refer disclaimer Overleaf.
Growth
Gas
HiMedia Laboratories
Bacillus cereus ATCC 10876 >=10

Escherichia coli ATCC
50-100
25922
Enterobacter aerogenes
50-100
ATCC 13048
Enterococcus faecalis ATCC 50-100
29212
Staphylococcus aureus
>=10
ATCC 25923
Technical Data
inhibited
good-luxuriant positive
reaction
good-luxuriant positive
reaction
none-poor
negative
reaction
inhibited
Storage and Shelf Life

Store below 30C in tightly closed container and the prepared medium at 2-8C. Use before expiry date on the label.
Reference
1. Greenberg A. E., Eaton A. D. and Clesceri L. S., (Eds.), 1998, Standard Methods for the Examination of Water and
Wastewater, 20th ed., APHA, Washington, D.C.
2. Downes F. P. and Ito K. (Eds.) 2001, Compendium of Methods for the Microbiological Examination of Food. 4th Ed, APHA,
Washington, D.C.
3. Richardson G., (Ed.), 1985, Standard Methods for the Examination of Dairy Products, 15th Ed, APHA, Washington, D.C.
4. McCrady and Langerin, 1932, J. Dairy Science, 15:321.
5. McCrady, 1937, Am. J. Publ. Health, 27:1243.
6. International Organization for Standardization (ISO), 1991, Draft ISO/DIS 4831.
Revision : 2 / 2015
Disclaimer :
User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in
this and other related HiMedia publications. The information contained in this publication is based on our research and development
work and is to the best of our knowledge true and accurate. HiMedia Laboratories Pvt Ltd reserves the right to make changes to
specifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use but
for laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not
be considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.
HiMedia Laboratories Pvt. Ltd. A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147
1919 Email: [email protected]

Himedia BGLB Broth 2% M121

Uploaded by

Copyright:

Available Formats

Himedia BGLB Broth 2% M121

Uploaded by

Document Information

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

Himedia BGLB Broth 2% M121

Uploaded by

Copyright:

Available Formats

M121

Brilliant Green Bile Broth 2%

Peptic digest of animal tissue

Principle And Interpretation

Bacillus cereus ATCC 10876 >=10

Storage and Shelf Life

You might also like