Adaptive Immunity

Hindawi Publishing Corporation
Journal of Diabetes Research

Volume 2013, Article ID 184258, 11 pages
http://dx.doi.org/10.1155/2013/184258
Research Article
Adaptive Immunity, Inflammation, and Cardiovascular
Complications in Type 1 and Type 2 Diabetes Mellitus
Daniela Pedicino,1 Giovanna Liuzzo,1 Francesco Trotta,1 Ada Francesca Giglio,1
Simona Giubilato,1 Francesca Martini,2 Francesco Zaccardi,2 Giuseppe Scavone,2
Marco Previtero,1 Gianluca Massaro,1 Pio Cialdella,1 Maria Teresa Cardillo,1
Dario Pitocco,2 Giovanni Ghirlanda,2 and Filippo Crea1
1
2
Institute of Cardiology, Catholic University, Largo A. Gemelli, 8-00168 Rome, Italy

Diabetes Care Unit, Internal Medicine, Catholic University, Largo A. Gemelli, 8-00168 Rome, Italy
Correspondence should be addressed to Giovanna Liuzzo; [email protected]

Received 5 March 2013; Accepted 5 April 2013
Academic Editor: Norman Cameron
Copyright 2013 Daniela Pedicino et al. This is an open access article distributed under the Creative Commons Attribution
License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly
cited.
Diabetes mellitus (DM) is a pandemics that affects more than 170 million people worldwide, associated with increased mortality and
morbidity due to coronary artery disease (CAD). In type 1 (T1) DM, the main pathogenic mechanism seems to be the destruction of
pancreatic -cells mediated by autoreactive T-cells resulting in chronic insulitis, while in type 2 (T2) DM primary insulin resistance,
rather than defective insulin production due to -cell destruction, seems to be the triggering alteration. In our study, we investigated
the role of systemic inflammation and T-cell subsets in T1- and T2DM and the possible mechanisms underlying the increased
cardiovascular risk associated with these diseases.
1. Introduction
Diabetes mellitus (DM) is a pandemics that affects more
than 170 million people worldwide [1, 2], associated with
increased mortality and morbidity due to coronary artery
disease (CAD) [3, 4]. Patients with DM have 2- to 4-fold
increase in risk of CAD and up to 3-fold increase in mortality
and carry the same level of risk for subsequent acute coronary
events as nondiabetic patients with previous myocardial
infarction (MI). DM also worsens early and late outcomes
in acute coronary syndromes (ACS) [5, 6]. Indeed, DM is
a prothrombotic condition, associated with inflammation,
altered innate immunity, and impaired endothelial function
[7, 8]. However, the mechanisms responsible for the higher
cardiovascular risk that accompanies DM are multiple and
still largely unknown.
In type 1 (T1) DM, the main pathogenic mechanism
seems to be the destruction of pancreatic -cells mediated
by autoreactive T-cells resulting in chronic insulitis. Thus, the
chronic inflammation of pancreatic islets plays a pivotal role

in the development of the disease [9].
In type 2 (T2) DM, primary insulin resistance, rather
than defective insulin production due to -cells destruction,
seems to be the triggering alteration. Insulin resistance
leads to a perturbation in the lipid homeostasis, cytokines,
and adipokines production, resulting in increased systemic
inflammation, with higher levels of inflammatory markers
such as C-reactive protein (CRP), interleukin (IL)-6, and
tumor necrosis factor (TNF)- [7, 1016].
Inflammation and immunity play also a key role in
the pathogenesis of atherosclerosis and its complications.
Immune responses are a cornerstone in the mechanisms of
endothelial activation, plaque development, and rupture, as
demonstrated by the correlations between levels of inflammatory markers or certain lymphocyte population and the
risk of occurrence of cardiovascular (CV) events [1721].
Several studies documented a role for T-cell subset imbalance
in the induction of an altered glucose homeostasis both in
2
T1DM and in T2DM [2242]. Recent experimental models
demonstrated that interferon (IFN)--producing cells are
present in elevated number in adipose tissue of obese mice
and have a role in promoting a loss in glucose homeostasis,
mostly derived by the induction of visceral adipose tissue
inflammation and endothelial dysfunction [24, 25]. Among T
lymphocytes, CD4+ CD28null cells represent a subpopulation
producing high levels of IFN-, showing resistance to apoptosis, and exerting direct cytolytic effects on endothelial cells
and proapoptotic effects on smooth muscle cells [2630]. Our
group has previously shown that circulating CD4+ CD28null
T-cell frequency higher than 4% is associated with a worse
outcome of ACS. Moreover, for the first time, we specifically
demonstrated that in T2DM patients CD4+ CD28null T-cells
are expanded and are associated both with the occurrence of
a first CV event and with a higher risk of recurrences after an
ACS [31, 32]. However, data on this particular T-cell subset
and its implication in diabetes are still lacking; particularly,
there are no studies exploring the role of CD4+ CD28null Tcells in T1DM and its complications.
The role of CD4+ CD25+ Foxp3+ regulatory T-cells (Tregs)
in both T1DM and T2DM has also been investigated. In
T2DM, Tregs seem to play a fundamental part in the regulation of body weight, adipocyte hypertrophy, glucose tolerance
insulin resistance, and thus in the disease progression [24,
33, 34]. Also, in T1DM, Tregs seem to be involved in the
onset and development of the disease, as suggested by studies documenting that alteration of Treg compartment may
cause or predispose to autoimmunity [3537]. The possible
mechanism linking Tregs, autoimmunity, and T1DM has
been evaluated in recent studies showing that Tregs in the
pancreas of NOD mice are prone to apoptosis and fail to
control autoimmune responses leading to diabetes [38]. Tregs
have also a role in the suppression of the inflammatory and
immune responses leading to CV diseases [39, 40]. Recently,
a powerful inhibition of atherosclerosis mediated by naturally
arising Tregs has been demonstrated in mouse models [41].
Nevertheless, the last decade has seen controversial reports
on defects in frequency or function of Tregs in T1DM [42].
Aim of the present study is to investigate the role of
systemic inflammation and T-cell subsets in T1 and T2DM
and the possible mechanisms underlying the increased CV
risk, which characterize these conditions.
2. Materials and Methods

2.1. Study Population
2.1.1. DM Patients. We enrolled 110 patients with a diagnosis
of DM defined according to ADA criteria, 55 of them affected
by T1DM and 55 patients with T2DM. Exclusion criteria were
(1) age > 80 years ( = 37); (2) evidence of inflammatory or infectious diseases, malignancies, immunologic, or
hematological disorders ( = 34); (3) treatment with antiinflammatory drugs other than low-dose aspirin ( = 32).
Clinical features were carefully recorded, including classical cardiovascular risk factors, age at diagnosis of DM, duration of DM, glycosylated haemoglobin A1c (HbA1c), and

body mass index (BMI). A complete cardiovascular screening
was performed, including a standard 12-lead EKG, a treadmill
EKG stress test, an Echo-color Doppler of carotid arteries,
the ankle brachial pressure index, and an Echo-color Doppler
to exclude lower-limb arterial disease. Microvascular complications, including diabetic nephropathy, neuropathy, and
retinopathy, were also assessed. To address the presence of
these possible complications, a complete neurological examination performed by certified independent neurologists, a
quantitative measurement of urine protein excretion in a
sample obtained from 24 h urine collection, and a fundoscopy
performed by certified independent ophthalmologists were
used.
2.1.2. Controls. As control group, we studied 60 individuals
without overt cardiovascular disease and DM, who were
screened in our outpatients clinic for cardiovascular prevention. We ascertained traditional cardiovascular risk factors
including age, blood pressure, smoking status, family history
of MI, and total, LDL, HDL cholesterol and triglycerides
levels.
All individuals gave their written informed consent.
The Ethics Committee of the catholic University of Rome
approved the study.
2.2. Anthropometric Measurements. Anthropometric measurements were taken according to standardized procedures
after an overnight fasting, with the patient wearing indoor
clothes without shoes. Height was measured in centimeters
using a stadiometer. Weight was measured with Tanita
bioimpedance balance (Tanita International Division, West
Dryton, UK). Waist circumference was measured just above
the uppermost lateral border of the right ileum using the
National Health and Nutrition Examination Survey protocol
[43].
2.3. Blood Sampling. Venous blood samples were taken at the
time of patient enrollment.
Total and differential white blood cell counts and T-cell
subset distribution were analyzed on fresh blood samples.
Whole blood samples were also used to assess HbA1c levels,
whereas coded serum samples were stored at 70 C and
analyzed for high-sensitivity C-reactive protein (hs-CRP) in a
single batch at the end of the study by laboratory staff unaware
of the clinical data.
2.4. T-Cell Analysis. Total and differential white blood cell
counts were obtained with a Bayer H 3-hematology analyzer
using automated cytochemistry in flow.
T-cell subsets were assessed by flow cytometry. Peripheral
blood mononuclear cells (PBMCs) were isolated from heparinized whole blood samples by standard gradient centrifugation over Ficoll-Hypaque (GE Healthcare Bio-Sciences,
Piscataway, NJ, USA), washed twice in PBS, then resuspended
to a density of 105 cells/mL, stained with the appropriate
monoclonal antibodies for cell surface staining, and fixed.
When indicated, PBMCs were permeabilized by permeabilization buffer (eBioscience, San Diego, CA, USA) for

intracellular staining. Isotype controls were given to enable
correct compensation and confirm antibody specificity. In
all cases, nonspecific staining with isotype-matched control
mAb was <1%; the intra- and interassay variability was <10%.
Analyses of stained cells were performed using an FC500
Flow Cytometry System (Beckman Coulter, Fullerton, CA,
USA). CXP ACQUISITION and CXP ANALYSIS software
packages (Beckman Coulter, Fullerton, CA, USA) were used
for data acquisition and analysis, respectively. All T-cell
subsets analyzed were expressed as a percentage of the entire
population of CD4+ T-cells.
2.4.1. Frequency of Different T-Cell Subsets. CD4+ CD28null
T-cell frequency was determined using anti-CD4-fluorescin
isothiocyanate- (FITC-) conjugated mAb and anti-CD28phycoerythrin- Cy5(PC5-) conjugated mAb (both Beckman
Coulter, Fullerton, CA, USA).
Treg cells were defined as CD4+ Foxp3+ T-cells. To this
purpose, PBMCs were stained with anti-CD4-FITC mAb.
After cell surface staining, cell fixation, and permeabilization,
cells were stained with the intracellular PE-conjugated antiFoxp3 mAB (eBioscience, San Diego, CA, USA) according to
the manufacturers instructions.
A cutoff value 4% was chosen to define patients with
high frequency of CD4+ CD28null T-cells, because 4% represents the 90th percentile of distribution in healthy individuals. This cutoff was also validated in our control group
(median = 1.5%; 90th percentile = 4.0%).
A cutoff value 5% was chosen to define patients with low
frequency of Tregs, because 5% represents the 10th percentile
of distribution in our control group (median = 7.8%; 10th
percentile = 5.0%).
The CD4+ CD28null /Treg balance was evaluated by the
ratio of CD4+ CD28null T-cells to CD4+ Foxp3+ T-cells; a
cutoff value 1.1 was chosen to define an alteration of the
balance, because this value represents the 99th percentile
of distribution in our control group (median = 0.21; 99th
percentile = 1.1%).
2.5. Measurements of hs-CRP and 1. We measured hsCRP concentrations using an ultrasensitive nephelometric
method (Siemens HealthCare Diagnostic BN System, Deerfield, IL, USA). The working range of the assay was 0.175
to 1100 mg/L, and the coefficient of variation was <5%. The
median normal value for hs-CRP was 0.8 mg/L, with 90% of
normal values <3 mg/L.
HbA1c was assessed by high-performance liquid chromatography, using Diamat BioRad (BioRad, Milan, Italy). The
HbA1c reference range was 4.35.9%.
2.6. Statistical Analysis. Because CD4+ CD28null and Treg
frequencies and hs-CRP values did not follow a normal
distribution, according to Kolmogorov-Smirnov, data were
expressed as median and range, and nonparametric tests
were used: the Kruskal-Wallis test with the Dunns multiple
pairwise comparison for comparisons among groups and
the Spearmans rank test for correlations. The remaining
3
continuous variables were expressed as mean SD and were
compared using 1-way ANOVA for repeated measures, with
the Bonferroni correction for multiple pairwise comparisons.
Proportions were compared using the chi-square test.
A two-tailed value <0.05 was considered statistically
significant. Statistical analysis was performed with SPSS 18.0
software (SPSS Inc., Chicago, Illinois, USA).
3. Results
Characteristics of study population are reported in Table 1.
As compared with both T2DM patients and controls,
T1DM patients were younger ( < 0.001 for both comparisons), and exhibited higher rate of hypercholesterolemia
( < 0.05 for both comparisons). As compared with T2DM,
T1DM patients had a longer disease duration ( = 0.020),
were more often treated with insulin ( < 0.001), and had
higher levels of total cholesterol ( = 0.045) and HDL
cholesterol ( < 0.001). They also had higher levels of HDL
cholesterol than controls ( < 0.001).
T2DM patients exhibited higher rate of family history
of IHD ( = 0.001 versus controls). As compared with
T1DM, T2DM patients were more often treated with oral
antidiabetic drugs ( < 0.001) and had higher BMI and
waist circumference ( < 0.001 for both comparisons),
higher triglycerides levels ( = 0.015), and higher rate of
macrovascular complications ( = 0.005).
3.1. Frequencies of T-Cell Subsets. No differences were found
in total T-cell and CD4+ T-cell counts among groups (Table 1).
CD4+ CD28null T-cell frequency was significantly higher
in T1DM than in T2DM and in controls ( = 0.001 and <
0.001, resp.) and it was higher in T2DM than in controls ( <
0.001) (Table 1 and Figure 1(a)).
In sharp contrast, Treg frequency was significantly lower
in T1DM than in T2DM and in controls ( < 0.001 for both
comparisons), and it was lower in T2DM than in controls
( < 0.001) (Table 1 and Figure 1(b)).
3.2. Imbalance between Effector and Regulatory T-Cells in
T1DM Patients. In T1DM, a statistically significant negative
correlation was detected between CD4+ CD28null T-cell frequency and Tregs ( = 0.28; = 0.015), likely suggesting
the preferential association of increased CD4+ CD28null Tcell immune response with impaired Treg function in single
T1DM patients (Figure 2(a)). No correlation was observed in
T2DM (Figure 2(b)).
The significance of increased CD4+ CD28null T-cell frequency and decreased Treg cells was further explored by calculating the CD4+ CD28null /Treg cell percentage ratio in each
subject. There was a strikingly higher CD4+ CD28null /Treg
ratio in T1DM patients than in T2DM patients and in controls
( < 0.001 for all comparisons), and a higher ratio in T2DM
patients than in controls ( < 0.001) (Table 1 and Figure 3).
3.3. Systemic Inflammation. We have also studied low-grade
systemic inflammation assessing the serum levels of hs-CRP.
Table 1: Clinical characteristics of study population.
Number of patients
Sex (M/F)
Age (mean SD)
Risk factors
Hypercholesterolemia, (%)
Hypertension, (%)
Smoke, (%)
Family history of IHD, (%)
Medications
Oral antidiabetic drugs, (%)
Insulin, (%)
Statins, (%)
Disease complications
Microvascular complications
(nephropathy, neuropathy, and retinopathy)
Macrovascular complications
(CAD, PVD, and cerebrovascular diseases)
Antropometric parameters (mean SD)
BMI (kg/m2 )
Waist circumference (cm)
HbA1c (%)
Mean duration of DM (years)
Laboratory assay (mean SD)
Total cholesterol (mg/dL)
LDL (mg/dL)
HDL (mg/dL)
Triglycerides (mg/dL)
Lymphocyte count (109 /L)
Total CD4+ T-cell frequency (%)
hs-CRP (mg/L), median (range)
Frequency of different T-cell subsets
Expressed as percentage of the entire CD4+ T-cell
population, median (range)
CD4+ CD28null T-cell (%)
CD4+ Foxp3+ T-cell (Treg) (%)
CD4+ CD28null /Treg ratio
T1DM
T2DM
Controls
value
55
37/18
45.2 14
55
36/19
62 10
60
34/26
55.7 11.8
0.118
0.037a
31 (56%)
26 (47%)
22 (40%)
26 (47%)
20 (36%)
37 (67%)
19 (35%)
37 (67%)
17 (28%)
48 (80%)
26 (43%)
18 (30%)
0.048b
0.015c
0.63
0.002d
8 (15%)
54 (98%)
25 (45%)
44 (80%)
15 (27%)
24 (44%)
NA
NA
7 (12%)
<0.001
<0.001
<0.001e
25 (45%)
28 (51%)
NA
0.33
18 (33%)
37 (67%)
NA
0.005
24.4 3.5
81 10
7.4 0.4
18.5 10.4
27.8 5.2
93 12.6
7.6 1.4
13.5 8.9
25.9 3.8
79 7
NA
NA
0.003f
0.002f
0.418
0.020
196.5 30
100.9 44.7
62 13.7
99.2 38.4
1.5 0.5
50.3 20.2
1.0 (0.29.1)
178.5 49.3
104.6 37.8
49.6 11
126.6 61.3
1.6 0.5
50.5 19.7
3.4 (0.221.9)
183.5 43.5
112.2 40.1
50.3 15.6
111.1 60.7
1.9 0.6
50.4 23.6
1.1 (0.27.9)
0.018g
0.65
<0.001a
0.029h
0.4
0.4
<0.001i
6.9 (0.432.8)
1.5 (0.53.3)
4.3 (0.726.4)
3.6 (0.222.5)
2.1 (0.312.3)
1.3 (0.166.5)
1.5 (0.28.0)
7.8 (4.112.0)
0.2 (0.11.3)
<0.001j
<0.001k
<0.001l
DM: diabetes mellitus; IHD: ischemic heart disease; CAD: coronary artery disease; PVD: peripheral vascular disease; BMI: body mass index; HbA1c:
glycosylated haemoglobin A1c; hs-CRP: high-sensitivity C-reactive protein.
a
< 0.001 T1DM versus T2DM and controls.
b
c
< 0.001 controls versus T1DM.
d
< 0.001 T2DM versus controls.
e
< 0.05 T1DM and T2DM versus controls.
f
g
< 0.05 T1DM versus T2DM.
h
< 0.05 T2DM versus T1DM.
i
j
< 0.001 T1DM (higher frequency) versus T2DM and controls; also, < 0.001 T2DM versus controls.
k
< 0.001 T1DM (lower frequency) versus T2DM and controls; also, < 0.001 T2DM versus controls.
l
< 0.001 T1DM versus T2DM and controls; also, < 0.001 T2DM versus controls.
< 0.001
< 0.001
< 0.001
< 0.001
< 0.001
= 0.0012
35
< 0.001
30
12
< 0.001
CD4+ Foxp3+ T-cell frequency (%)
CD4+ CD28null T-cell frequency (%)
13
25
20
15
10
5
11
10
9
8
7
6
5
4
3
2
1
0
0
T1DM
( = 55)
T2DM
( = 55)
T1DM
( = 55)
HC
( = 60)
(a)
T2DM
( = 55)
HC
( = 60)
(b)
Figure 1: Frequencies of T-cell subsets in different groups. T1DM and T2DM groups were defined according to ADA criteria, while controls
were individuals without overt cardiovascular disease and DM. Frequencies of T-cells were determined by two-color flow cytometry. Data
are presented as single data points. (a) CD4+ CD28null T-cell frequencies in different groups. CD4+ CD28null T-cell frequency was significantly
higher in T1DM than in other groups, and it was higher in T2DM than in controls. (b) CD4+ FoxP3+ T-cell frequencies in different groups.
CD4+ FoxP3+ T-cell frequency was significantly lower in T1DM than in other groups, and it was lower in T2DM than in controls.
T2DM ( = 55)
T1DM ( = 55)
= 0.28; = 0.015
35
= 0.21; = 0.11
24
22
20
30
25
20
15
10
18
16
14
12
10
8
6
4
2
0
0
0
2
3
4
(a)
4
6
8
10
(b)
Figure 2: Correlation between CD4+ CD28null T-cell and CD4+ FoxP3+ T-cell frequencies in T1DM and T2DM patients. Data are presented as
single data points. (a) In T1DM patients, a significant negative correlation was found between CD4+ CD28null T-cell and CD4+ FoxP3+ T-cell
frequencies. (b) In T2DM patients, no correlation was found between CD4+ CD28null T-cell and CD4+ FoxP3+ T-cell frequencies.

< 0.001
< 0.001
< 0.001
30
= 0.99
< 0.001
< 0.001
< 0.001
22
20
18
16
20
15
< 0.001
Hs-CRP (mg/L)
CD4+ CD28null /CD4+ Foxp3+ T-cell ratio
25
14
12
10
8
6
4
10
2
0
T1DM
( = 55)
0
T1DM
( = 55)
T2DM
( = 55)
HC
( = 60)
Figure 3: CD4+ CD28null /CD4+ FoxP3+ percentage ratio in different

groups. T1DM, T2DM, and control groups were defined as in Figure 1. Data are presented as single data points. A significantly higher
CD4+ CD28null /CD4+ FoxP3+ ratio was detected in T1DM patients
than in T2DM and in controls. CD4+ CD28null /CD4+ FoxP3+ ratio
was also higher in T2DM patients than in controls.
Hs-CRP was significantly higher in T2DM than in the other

groups ( < 0.001 for all comparisons). Of note, T1DM
patients had similar hs-CRP levels than controls ( = 0.99)
(Table 1, Figure 4).
3.4. T-Cell Frequencies and hs-CRP Levels according to
Metabolic Factors, Disease Duration, and Complications. In
T1DM patients, no correlations were observed between
CD4+ CD28null T-cell and/or Treg frequency and metabolic
factors, disease duration, and complications.
In T2DM patients, CD4+ CD28null T-cell frequency was
positively correlated ( = 0.47; < 0.001), and Treg
frequency was negatively correlated ( = 0.41; = 0.002)
with disease duration (Figures 5(a) and 5(b)); moreover,
CD4+ CD28null T-cell frequency was positively correlated
( = 0.41; = 0.008), and Treg frequency was negatively
correlated ( = 0.52; = 0.001) with HbA1c (Figures 6(a)
and 6(b)); finally, a statistically significant correlation was
found between hs-CRP levels and BMI ( = 0.47; < 0.001)
(Figure 7).
To investigate the potential relationship between CD4+ Tcell subset alteration with the types of diabetic complications,
we compared the levels of CD4+ CD28null T-cells and Tregs in
T1DM and T2DM patients with or without different types of
complications. No differences were observed among T1DM
T2DM
( = 55)
HC
( = 60)
Figure 4: Serum hs-CRP levels in different groups. Data are presented as single data points. Serum hs-CRP levels were significantly
higher in T2DM than in the other groups. No difference was found
between T1DM patients and controls.
patients with or without micro- or macrovascular complications. CD4+ CD28null T-cells and Treg were also similar
in T2DM patients with or without micro- or macrovascular complications, while hs-CRP levels were significantly
higher in T2DM patients with macrovascular complications
(median 4.3, range 0.521.9 versus median 2.0, range 0.514.6;
= 0.047).
These data suggest that the changes of CD4+ T-cell subsets
in T1DM patients may happen before the occurrence of
complications and at the early stage of disease. In contrast, in
T2DM patients, CD4+ T-cell subset alteration might be the
consequence of disease duration and metabolic control.
4. Discussion
DM and CAD are two pathological conditions closely related
to each other; as demonstrated by the fact that, in the
evaluation of cardiovascular risk, DM is considered as an
equivalent of established CAD [3, 4]. Atherothrombosis is the
leading cause of death among diabetic patients, accounting
for about 80% of mortality in this population, which comprehends more than 150 million people worldwide; therefore,
the burden of DM-related CAD constitutes a major health
problem [3, 4].
Several mechanisms could be involved in the pathogenesis of diabetes-related vascular complications, including
oxidative stress, vascular damage due to advanced end glycation products (AGE), and glucose-induced inflammation
[7, 8, 13, 14].
It is well established that both T1DM and T2DM are
associated with a systemic inflammatory state [4446]. The
main features of inflammation, however, could be different
between T1DM and T2DM, due to their different pathogenesis. In fact, T1DM onset is related to pancreatic -cells

= 0.47; < 0.001
26
= 0.41; = 0.002
13
24
12
22
11
20
10
18
16
14
12
10
8
6
9
8
7
6
5
4
3
1
0
0
0
10
15
20
25
30
Disease duration (years)
35
40
10
20
30
Disease duration (years)
(a)
40
(b)
Figure 5: Correlation between T-cell subsets and disease duration in T2DM patients. T2DM patients were defined as in Figure 1. Data
are presented as single data points. (a) CD4+ CD28null T-cell frequency positively correlates with disease duration in T2DM patients. (b)
CD4+ FoxP3+ T-cell negatively correlates with disease duration in T2DM patients.
= 0.52; = 0.001
= 0.41; = 0.008
13
26
12
11
22
24
20
18
16
14
12
10
8
6
10
9
8
7
6
5
4
3
0
4
8
9
HbA1c
10
11
12
13
(a)
10
11
12
13
HbA1c
(b)
Figure 6: Correlation between T-cell subsets and serum HbA1c levels in T2DM patients. T2DM patients were defined as in Figure 1. Data
are presented as single data points. (a) CD4+ CD28null T-cell frequency positively correlates with HbA1c in T2DM patients. (b) CD4+ FoxP3+
T-cell negatively correlates with HbA1c in T2DM patients.
destruction by autoreactive T-cells and subsequent insulin

deficiency leading to the metabolic alterations of the disease
[9, 45], while the pathogenesis of T2DM involves primarily
insulin resistance, rather than absolute insulin deficiency,
deriving from metabolic alterations in insulin-responsive
tissues and resulting in a complex alteration of lipid and

glucose homeostasis [17, 18]. These perturbations lead to an
imbalance in cytokines and adipokines production resulting
in increased systemic inflammation [13, 14]. Conversely,
systemic inflammation can itself predispose to the onset of

= 0.47; < 0.001
25
20
20
Hs-CRP (mg/L)
Hs-CRP (mg/L)
= 0.008
25
15
10
15
10
0
20
25
30
BMI (kg/m2 )
35
(a)
40
BMI
<30
BMI
30
(kg/m2 )
(b)
Figure 7: Correlation between serum hs-CRP levels and BMI in T2DM patients. T2DM patients were defined as in Figure 1. Data are presented
as single data points. (a) Positive correlation was statistically found between serum hs-CRP levels and BMI in T2DM patients. (b) Obese T2DM
patients (BMI 30 kg/m2 ) showed higher levels of serum hs-CRP than nonobese (BMI < 30 kg/m2 ) T2DM patients.
insulin resistance and consequently T2DM, as shown by several studies demonstrating that high levels of inflammation
markers (including CRP, fibrinogen, IL-6, and plasminogen
activator inhibitor PAI-1) could predict the risk of developing
T2DM [7, 8, 11, 12]. CRP is now recognized as a sensitive
and useful, although nonspecific, marker of cardiovascular
risk [16]. In our work, we found higher levels of hs-CRP
in T2DM patients, with values of hs-CRP approximately
similar in T1DM and in controls. Moreover, we observed
higher levels of hs-CRP in those T2DM patients with overt
macrovascular complications. Recent studies highlighted the
correlation between high levels of CRP and IL-1 in diabetic
patients. A statistically significant reduction in CRP, IL-6,
and other inflammatory biomarkers was observed after the
administration of anti-IL-1 antibodies in T2DM patients,
suggesting a prominent role of this cytokine in the induction
of the chronic inflammatory state characterizing the disease
[47, 48] and its possible involvement in the subsequent
alteration of the adaptive immune response [49].
The role of the adaptive immune system has also been
investigated in the pathogenesis of T1DM and T2DM. Particularly, a disregulation of T-cell compartment seems to be a
pivotal feature of both the diseases.
CD4+ CD28null T-cells, extremely unusual in healthy
adults, are expanded in chronic inflammatory disorders
such as rheumatoid arthritis. These T-cells have a prominent Th1 phenotype, producing high levels of IFN-, are
resistant to apoptosis, and represent a marker of senescence of the immune system [26, 27]. In the atherosclerotic
plaque microenvironment, CD4+ CD28null T-cells have direct
cytolytic effects on endothelial cells and proapoptotic effects
on smooth muscle cells; thus, they might contribute to

plaque instability [29, 30]. In ACS, CD4+ CD28null T-cell
frequency has been associated with the recurrence of acute
coronary events [31]. In DM patients, CD4+ CD28null T-cells
are expanded and are associated with poor glycemic control,
with the occurrence of a first cardiovascular event and with a
worse outcome after an ACS [32].
At the other extreme, CD4+ CD25+ Foxp3+ regulatory Tcells (Tregs) have a key role in the suppression of pathological
inflammatory and immune responses. Their frequency is
reduced in several autoimmune diseases and also in ACS
[40, 5052]. Recently, we have observed that a large subset
of ACS patients presents a unique adaptive immunity system
signature, associated to a worse outcome and characterized by
inadequate Treg response to effector T-cell expansion [52].
Although CD4+ CD28null T-cells have been implicated in
several immunological disorders, no previous studies have
assessed the role of this T-cell subset in T1DM. More importantly, in the present study, we have systematically explored
the balance between aggressive effector T cells/regulatory
T cells by comparing patients with T1DM and T2DM. An
altered immune balance has been found in both T1DM and
T2DM, although with substantial differences.
In the present study, T1DM patients were characterized
by higher CD4+ CD28null T-cell frequency and lower Treg
frequency, by a strikingly higher CD4+ CD28null /Treg ratio,
and by a negative correlation between CD4+ CD28null T-cells
and Tregs. These findings suggest the preferential association
of increased CD4+ CD28null T-cell immune response with
impaired Treg function in single T1DM patients. All together,

our data suggest that, in the altered response of adaptive
immunity involved in the pathogenesis of T1DM, a role
might be reserved to special subset of T-cells, which could
represent both the triggering factor and the continuing
stimulus sustaining the disease. On the other hand, in T2DM,
an imbalance in the adaptive immunity seems to appear
later in the course of the disease as a consequence of a
chronic systemic inflammatory state that is involved both
in the pathogenesis of the disease and in its macrovascular
complications. In fact, an altered adaptive immunity is
also present in T2DM patients with CD4+ CD28null T-cell
frequency higher and Treg frequency lower than in controls,
but it seems to be strictly related to disease duration and
glycemic control rather than being a mechanism involved
in the early phase of the disease. In our previous study, we
demonstrated that the expansion of CD4+ CD28null T-cells
in T2DM patients is independently associated with HbA1c
levels and not with fasting glucose [32]. This suggests that the
development of unusual T-cell subsets might be influenced
by persistent poor glycemic control and might be presumably
related to the presence of high levels of AGEs, known to be
implicated in the onset of DM vascular complications [14].
5. Conclusions
Our study demonstrates that both T1DM and T2DM are
associated with an impaired T-cell balance, characterized
by CD4+ CD28null T-cell expansion and CD4+ CD25+ Foxp3+
regulatory T-cell reduction. However, the meaning of these
imbalances might be different between the two diseases. In
fact, the altered adaptive immunity found in T1DM seems
to be both the triggering factor and the continuing stimulus
sustaining the disease, while in T2DM the same disregulation
seems to appear later in the course of the disease, as
consequence of a persistent poor glycemic control and of a
chronic systemic inflammatory state which is associated with
overt macrovascular complications.
Our results pave the way to a novel explanation for the
possible role of adaptive immune system in the development
of T1DM and T2DM, which could also have important
implications in the prevention and treatment of diabetes and
its cardiovascular complications. Interestingly, recent trials
showed that IL-1 neutralising antibodies improve glycemic
control and reduce inflammation in patients with T2DM
[47, 48]. Further studies are needed to ascertain whether the
defective number of Tregs is part of the causation leading to
T1DM or an epiphenomenon. In the meantime, a number
of immunomodulatory intervention trials have now been
conducted in patients at risk for or with recent onset T1DM,
often with the goal of restoring immune tolerance by inducing
Tregs [5355].
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Institute of Cardiology, Catholic University, Largo A. Gemelli, 8-00168 Rome, Italy

Correspondence should be addressed to Giovanna Liuzzo; [email protected]

chronic inflammation of pancreatic islets plays a pivotal role

2. Materials and Methods

Journal of Diabetes Research

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Table 1: Clinical characteristics of study population.

Journal of Diabetes Research

CD4+ CD28null T-cell frequency (%)

CD4+ CD28null T-cell frequency (%)

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CD4+ CD28null /CD4+ Foxp3+ T-cell ratio

Figure 3: CD4+ CD28null /CD4+ FoxP3+ percentage ratio in different

Hs-CRP was significantly higher in T2DM than in the other

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CD4+ Foxp3+ T-cell frequency (%)

CD4+ CD28null T-cell frequency (%)

CD4+ CD28null T-cell frequency (%)

destruction by autoreactive T-cells and subsequent insulin

tissues and resulting in a complex alteration of lipid and

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on smooth muscle cells; thus, they might contribute to

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Journal of Diabetes Research

Journal of Diabetes Research

Hindawi Publishing Corporation

Hindawi Publishing Corporation

Research and Treatment

Hindawi Publishing Corporation

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Submit your manuscripts at

Hindawi Publishing Corporation

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Oxidative Medicine and

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