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    List of Culture Media Used in Microbiology With Their Uses

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    Immu Ji
    This document lists various culture media used in microbiology and their purposes. It provides over 50 examples of media including their uses for isolating, selectively growing, or differentiating between various microorganisms such as bacteria, viruses, parasites. Common media include blood agar, MacConkey agar, thioglycollate broth and are used to support the growth of aerobic/anaerobic organisms or for biochemical tests. Specialized media help isolate specific pathogens like Campylobacter, Salmonella, Streptococcus and Mycobacterium.

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    List of Culture Media Used in Microbiology With Their Uses

    Uploaded by

    Immu Ji
    3K views7 pages
    This document lists various culture media used in microbiology and their purposes. It provides over 50 examples of media including their uses for isolating, selectively growing, or differentiating between various microorganisms such as bacteria, viruses, parasites. Common media include blood agar, MacConkey agar, thioglycollate broth and are used to support the growth of aerobic/anaerobic organisms or for biochemical tests. Specialized media help isolate specific pathogens like Campylobacter, Salmonella, Streptococcus and Mycobacterium.

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    This document lists various culture media used in microbiology and their purposes. It provides over 50 examples of media including their uses for isolating, selectively growing, or differentiating between various microorganisms such as bacteria, viruses, parasites. Common media include blood agar, MacConkey agar, thioglycollate broth and are used to support the growth of aerobic/anaerobic organisms or for biochemical tests. Specialized media help isolate specific pathogens like Campylobacter, Salmonella, Streptococcus and Mycobacterium.

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    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    3K views7 pages

    List of Culture Media Used in Microbiology With Their Uses

    Uploaded by

    Immu Ji
    This document lists various culture media used in microbiology and their purposes. It provides over 50 examples of media including their uses for isolating, selectively growing, or differentiating between various microorganisms such as bacteria, viruses, parasites. Common media include blood agar, MacConkey agar, thioglycollate broth and are used to support the growth of aerobic/anaerobic organisms or for biochemical tests. Specialized media help isolate specific pathogens like Campylobacter, Salmonella, Streptococcus and Mycobacterium.

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    List of culture media used in

    microbiology with their uses


    Media
    Alkaline Peptone Water
    Alkaline Salt Transport Medium
    Taurocholate Peptone Transport Medium

    Uses
    Enrichment media for Vibrio cholerae
    Transport media for diarrheal diseases
    suspected of being caused by V. cholerae
    Liquid media by addition of

    Anaerobic Media

    Glucose (0.5 % to 1 %)

    Ascorbic Acid (0.1 %)

    Cysteine (0.1 %)

    Sodium Merceptoacetate (0.1 %)

    Thioglycollate (0.1 %)

    Particles of cooked meat broth

    Bile Salt Agar


    Thiosulphate Citrate Bile Salts-Sucrose
    Agar (TCBS)

    Selective media for V. cholerae

    Monsurs Tellurite Taurocholate Gelatin Agar

    Bile Esculin Agar (Contains 40% Bile)


    Blood Agar

    Selective media for Enterococcus species


    (Black coloration of the medium)

    Enriched media (Supports the growth of

    fastidious organisms, e.g. Streptococcus)

    Indicator media to show hemolytic

    properties of certain organisms


    (Staphylococcus aureus: -

    Hemolytic; Streptococcus pneumonia and S


    viridans: -Hemolytic; Enterococcus: NonHemolytic)

    Bordet-Gengou Agar
    Charcoal Blood Agar
    Regan-Lowe Medium (Charcoal Agar with

    Isolation of Bordetella pertussis

    blood, cephalexin and Amphotericin B)

    Brain Heart Infusion Broth

    Used in blood culture bottles (both adult and


    pediatric patients)

    Buffered Charcoal Yeast Agar (BCYA)


    Feeley Gorman Agar

    Campylobacter Thioglycollate Broth

    Specialized media for isolation of Legionella

    Selective holding media for recovery


    ofCampylobacter species

    Castaneda Medium

    Biphasic medium for the isolation of Brucella

    Cefoxitin Cycloserine Fructose Agar (CCFA)

    Selective media for isolation of Clostridium

    Cefoxitin Cycloserine Egg-Yolk Agar


    (CCEY)

    difficile form suspected cases of


    pseudomembranous colitis/antibioticassociated diaeehea

    Cefsulodin-Irgasan-Novobiocin medium

    Selective media for Yersinia (and may be used

    (CIN Medium)

    forAeromonas also)

    Columbia-Colistin Nalidixic Acid agar (CNA

    Selective media for the isolation of Gram-

    Agar)

    positive cocci

    Cooked meat broth

    In general, used for preservation and storage

    Nutrient agar slopes

    of bacterial cultures

    Semisolid nutrient agar stabs


    Heated blood agar slopes
    Crystal violet blood agar

    Selective media for Streptococcus pyogenes

    Cysteine Lactose Electrolyte Decient

    Most commonly used media for culturing urine

    Media (CLED Media)

    samples

    Egg Saline Medium

    Egg Yolk Agar

    Preservation of cultures of Gram-negative


    bacilli
    Detection of lipase and lecithinase activity
    ofClostridium species

    Ellners Medium
    Medium of Duncan and Strong
    Medium of Phillips

    Specialized media to induce sporulation


    inClostridium

    Alkaline Egg Medium


    Fildes Blood-Digest Agar and Broth
    Levinthals Agar

    Firm Agar (4% to 6% Agar)

    Enriched media for recovery of Haemophilus


    inuenzae
    Prevents swarming of Proteus mirabilis, P.
    vulgarisand Clostridium tetani

    Fletchers Agar
    Ellinghausen and McCullough Medium
    Ellinghausen-McCullough-Johnson-Harris

    Solid media for isolation of Leptospira

    (EMJH) Media
    Glycerol Saline Transport Medium

    Transport stool specimen for typhoid bacilli

    Heated Blood Agar/Chocolate Agar

    Growth of fastidious organisms

    (E.g. Hemophilus inuenzae, Neisseria


    gonorrhoeae and S. pneumoniae
    Hoyles Tellurite Lysed Blood Agar
    Tinsdale Medium

    Loefer Serum Slope


    Lowenstein-Jensen Medium
    Middlebrook Media

    Selective media for isolation


    of Corynebacteriumfrom throat swabs
    Stimulation of metachromatic granules
    inCorynebacterium diphtheriae
    Selective media for isolation of Mycobacterium
    tuberculosis from sputum and other samples
    Differential media for Enterobacteriacaeae

    MacConkey Agar

    (i.e., lactose fermenting and non-lactose


    fermenting)

    MacConkey Bile Salt Lactose Agar


    Brilliant MacConkey Agar
    Leifsons Deoxycholate-Citrate Agar (DCA)
    Wilson and Blairs Brilliant Green BismuthSulphite Agar (BBSA)
    Taylors Xylose Lysine Deoxycholate Agar

    Differential and media for isolation


    of Salmonellaand Shigella from stool
    specimens

    (XLD)
    Hektoen Enteric Agar
    SalmonellaShigella Agar
    Mannitol Salt Agar
    Modied Barbour Stoenner Kelly medium
    (BSK)
    Modied Korthoffs Medium

    Selective and indicator media for S. aureus


    Specialized media for Borrelia burgdorferi
    Liquid media for isolation of Leptospira

    Modied New York City Medium (contains


    colistin, lincomycin, trimethoprim, amphotericin

    Selective media for Neisseria gonorrhoeae

    B)
    Mueller-Hinton Agar

    Performing antimicrobial susceptibility for


    bacteria
    Basal media in microbiology

    Nutrient Agar (1% to 2% Agar)

    Supports the growth of all non-fastidious


    organisms

    Non-Nutrient Agar

    Cultivation of parasites (e.g., Acanthamoeba)

    Basal media for preparation for

    carbohydrate fermentation media

    Peptone Water

    non-motile

    Phenol-Red Egg Yolk Polymyxin Agar

    To ascertain whether a bacteria is motile or

    Basis for Indole test

    Selective media for isolation of Bacillus


    cereusfrom food, feces, and vomitus
    Preservation of S. pyogenes, pneumococci,

    Pikes Media

    andHemophilus inuenzae in nose and throat


    swabs
    Selective media for isolation of Bacillus

    Polymyxin B-lysozyme-EDTA-Thallous

    anthracisfrom soil and other medium materials

    Acetate (PLET)

    containing numerous spore formers of other


    species

    Polymyxin B, Neomycin, Fusidic Acid Media

    Selective media for S. pyogenes (or -

    (PNF)

    hemolyticStreptococcus)

    PPLO Medium (Contains Sterol)

    Specialized media for Mycoplasma


    pneumoniae

    Pre-Reduced Anaerobically Sterilized

    Commercially available media for anaerobic

    (PRAS) Media

    organisms

    Proteose Peptone-Yeast Extraction Broth

    Media for carrying out biochemical tests for


    anaerobes

    Requirements of X and V Factors

    Isolation of Haemophilus inuenzae

    Roswell Park Memorial Institute (RPMI)

    Cultivation of malarial parasites

    1640 Medium

    (i.e., Plasmodium)

    Robertson Cooked Meat Broth (RCMB)

    Growth of anaerobes (e.g., Clostridium)

    Maintaining stock cultures of anaerobic

    organisms
    Salt-Cooked Meat Broth (Cooked Meat

    Enrichment media for isolation of S.

    Broth with 10% NaCl)

    aureus from heavily contaminated materials


    Prevents convection current and allows the

    Semisolid Agar (0.05% to 0.1% Agar)

    growth of anaerobic and micro-aerophilic


    organisms

    Skirrows Campylobacter Medium (contains


    polymixin B, trimethoprim, vancomycin)
    Preston Campylobacter Medium (contains
    polymixin B, rifampicin, trimethoprim)

    Selective media for Campylobacter jejuni

    Campy Blood Agar


    CVA Medium (contains cefoperazone,
    vancomycin, amphotericin)

    Smith-Noguchi Medium

    Cultivation of nonpathogenic treponemes (e.g.,


    Reiter strain of Treponema phagedenis)

    Isolation of verocytotoxin-producing
    Sorbitol MacConkey Agar

    (enterohemorrhagic) E. coli of 0157 type (as it


    fails to ferment D-sorbitol)

    Stuart Transport Media


    Amies Transport Media

    Maintaining the viability of gonococci on swabs


    during transportation

    Tetrathionate Broth
    Gram-Negative Broth
    Selenite-F Broth
    Thayer-Martin Medium (contains
    vancomycin, colistin, nystatin)
    Thioglycollate Broth
    Trypticase Soy Broth

    Todd Hewitt Broth with Antibiotics

    Triple Sugar Iron Agar (TSI) Medium

    Enrichment media for isolation


    of Shigella andSalmonella from stool samples

    Selective media for Neisseria gonorrhoeae


    All purpose enrichment broth for anaerobes,
    aerobes, micro-aerophilic, and fastidious
    organisms
    Selective and enrichment for Streptococccus
    agalactiae in female genital specimens
    Differentiation of various members of
    Enterobacteriaceae
    Performing antimicrobial susceptibility of

    Wilkins-Chalgren Agar

    anaerobic bacteria

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