Operating Manual Centrifuge 5418 5418 R

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www.eppendorf.com/myeppendorf
Centrifuge 5418 / 5418 R

Operating Manual
Copyright 2013 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior
permission of the copyright owner.
Trademarks
Eppendorf and the Eppendorf logo are registered trademarks of Eppendorf AG.
Microtainer is a registered trademark of Becton Dickinson, Franklin Lakes, NJ, USA.
Trademarks are not marked in all cases with or in this manual.
5401 900.022-07/102013
Table of contents
English (EN)
Table of contents
p.
Tab.
Operating
Centrifuge
on
see
Fig.
p.
manual
5418
/ 5418 R
English
Operating
(EN)
manual
Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.1
Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2
Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2.1
Danger symbols. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2.2
Danger levels. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.3
Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.4
Abbreviations used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.1
Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.2
Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.2.1
Centrifuge 5418. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.2.2
Centrifuge 5418 R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.3
Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.4
Rotor FA-45-18-11. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.4.1
rcf display and calculation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
3.1
Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
3.2
User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
3.3
Application limits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
3.3.1
Declaration concerning the ATEX directive (94/9/EC) . . . . . . . . . . . . . . . . . . . . . . . . . 11
3.3.2
Maximum service life for accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
3.4
Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
3.5
Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
3.5.1
Personal injury or damage to the equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
3.5.2
Incorrect handling of the centrifuge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
3.5.3
Incorrect handling of the rotors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
3.5.4
Extreme strain on the centrifuging tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
3.5.5
Aerosol-tight centrifugation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
3.6
Safety instructions located on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
4.1
Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
4.2
Preparing installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
4.3
Installing the instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
5.1
Overview of operating controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
5.2
Preparing for centrifugation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
5.2.1
Switching on the centrifuge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
5.2.2
Inserting the rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
5.2.3
Loading the rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
5.2.4
Closing the rotor lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
5.2.5
Closing the centrifuge lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Table of contents
English (EN)
5.3
5.4
5.5
5.6
Cooling (only 5418 R) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

5.3.1
Temperature adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5.3.2
Temperature display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5.3.3
Temperature monitoring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5.3.4
FastTemp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
5.3.5
Continuous cooling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Centrifuging . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
5.4.1
Centrifugation with time setting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
5.4.2
Centrifuging in continuous operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
5.4.3
Short-spin centrifugation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
5.4.4
Removing the rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Standby mode (only 5418) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Special functions (only 5418 R) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
6.1
Prepare cleaning/disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
6.2
Cleaning/disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
6.2.1
Cleaning and disinfecting the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
6.2.2
Cleaning and disinfecting the rotor. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
6.3
Additional service instructions for Centrifuge 5418 R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
6.4
Glass breakage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
6.5
Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
6.6
Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
7.1
General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
7.2
Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
7.3
Emergency lid release . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
7.3.1
Centrifuge 5418. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
7.3.2
Centrifuge 5418 R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

8.1
Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
8.2
Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
8.3
Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
9.1
Power supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
9.2
Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
9.3
Weight/dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
9.4
Application parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
10 Ordering information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
10.1 Centrifuge 5418. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
10.2 Centrifuge 5418 R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
10.3 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
10.4 Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
10.4.1 Fuses for Centrifuge 5418. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
10.4.2 Fuses for Centrifuge 5418 R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Operating instructions
English (EN)
1.1
Using this manual
Read this operating manual completely before using the device for the first time. Please also note the
operating instructions for the accessories, if applicable.
This operating manual is part of the product. Thus, it must always be easily accessible.
Enclose this operating manual when transferring the device to third parties.
If this manual is lost, please request another one. You will find the current version on our webpage
www.eppendorf.com/worldwide.
1.2
Danger symbols and danger levels
The safety instructions in this manual appear with the following danger symbols and danger levels:
1.2.1
Danger symbols
1.2.2
Biohazard
Explosion
Electric shock
Crushing
Hazard point
Material damage
Danger levels
DANGER
Will lead to severe injuries or death.
WARNING
May lead to severe injuries or death.
CAUTION
May lead to light to moderate injuries.
NOTICE
May lead to material damage.
1.3
Symbols used
Symbol
Meaning
Handling
1.
2.
Actions in the specified order
Text
List
Name of fields in the software
Useful information
English (EN)
1.4
Abbreviations used
PCR
Polymerase chain reaction
RZB/rcf
Relative centrifugal force g-force in m/s2
rpm
Revolutions per minute in rpm
UV
Ultraviolet radiation
Product description
English (EN)
Product description
2.1
Main illustration
Abb. 2-1: Front and rear view of the Centrifuge 5418
1
2
1
2
3
4
6
7
8
9
5
Fig. 2-1:
Front and rear view of the Centrifuge 5418
Abb. 2-2: Front and rear view of the Centrifuge 5418 R
1
2
1
2
5
3
4
6
9
10
10
7
8
Fig. 2-2:
Front and rear view of the Centrifuge 5418 R
1 Centrifuge lid
6 Name plate
2 Monitoring glass
Visual control for rotor stop or speed control
option using stroboscope
7 Mains/power switch
Switch for switching the device on and off.
Switch position 0: The device is switched off.
Switch position I: The device is switched on.
3 Display
Depiction of the current centrifuging parameters 8 Mains connection
and device settings.
Connection socket for the mains cable supplied.
4 Control panel
For operating the centrifuge.
5 Emergency lid release
9 Fuse holder
10 Condensation water tray (only Centrifuge 5418
R)
Product description
English (EN)
2.2
Delivery package
2.2.1
Centrifuge 5418
Quantity Order no.

Order no.
(International) (North America)
1
1
or
5425 351.003
5425 353.006
022668188
022668226
5416 301.001
022634305
1
1
5401 900.022
5401 900.030
5401900022
5401900030
2.2.2
Description
Centrifuge 5418
See chapter Ordering Information for corresponding
device version, equipment and order number
Fuse
2.5 A T (230 V), set of 2
5 A T (120 V), UL, set of 2
Rotor key
Standard
Power supply device
Operating manual Centrifuge 5418/5418 R
Languages: EN, DE, FR, ES, IT, PT
Languages: DA, FI, EL, NL, SV (230 V devices only)
Centrifuge 5418 R
Quantity Order no.

Order no.
(International) (North America)
1
1
or
5425 351.003
5426 355.100
022668188
022668200
5416 301.001
022634305
5401 850.076
5401850076
1
1
5401 900.022
5401 900.030
5401900022
5401900030
Description
Centrifuge 5418 R
See chapter Ordering Information for corresponding
device version, equipment and order number
Fuse
2.5 A T (230 V), set of 2
6.25 AT (100 V/120 V), set of 2
Rotor key
Standard
Tray for condensation water
Power supply device
Operating manual Centrifuge 5418/5418 R
Languages: EN, DE, FR, ES, IT, PT
Languages: DA, FI, EL, NL, SV (230 V devices only)
Product description
English (EN)
2.3
Features
The compact and easy-to-use Centrifuge 5418 / 5418 R has a capacity of 18 x 2 ml and reaches a maximum
of 16,873 x g / 14,000 rpm. The microcentrifuge is equipped with an aerosol-tight standard rotor for
centrifugation of the following tubes:
Micro test tubes (0.2 to 2.0 ml)
Microtainers (0.6 ml)
Spin columns (1.5/2.0 ml)
The Centrifuge 5418 R has an additional temperature control function for centrifugation between 0C and
+40C. The fast temp function can be used to start a temperature control run without samples to adjust the
rotor chamber incl. rotor and adapters quickly to the set target temperature.
2.4
Rotor FA-45-18-11
Before using tubes, observe the manufacturer's recommended specifications on resistance to

centrifugation (max. g-force).
Max. capacity
Max.
g-force (rcf)
or
speed (rpm)
without
adapter
Notes
Max. load
per rotor
bore(1)
Acceleration/deceleration
time (2)
Rotor
FA-45-18-11
18 micro test tubes of 1.5/

2.0 ml each or spin columns.
With adapters:
0.2 mL PCR tubes
0.4 ml/0.5 ml micro test
tubes
0.6 mL Microtainers
16,873 x g /
14,000 rpm
3.75 g
Aerosol-tight(3)
rotor lid
(aluminum).
5418: 16 s / 18 s
5418 R: 13 s / 13 s
(1) Maximum load per rotor bore for adapter + tube + contents.
(2) According to DIN 58 970 (device version: 230 V, 50 to 60 Hz).
(3) Aerosol tightness tested and certified by the Centre of Emergency Preparedness and Response, Health
Protection Agency, Porton Down (UK) (see certificates at the end of this operating manual).
Rotor code:
All Eppendorf rotors are identied
using a simple, alphanumeric
format that represents the technical
specications in a uniform series of
letters and numbers.
Fixed-angle
rotor
F A
Aerosol-tight
version
Angle of
bores
45
30
Number of
bores
of
bores (mm)
Swing-bucket
rotor
11
of
buckets (mm)
4
Number of
buckets
81
10
Product description
English (EN)
2.4.1
rcf display and calculation

Use the rpm/rcf key to switch the display of centrifugation speed between rcf and rpm.
Only 5418 R: For speeds 800 rpm only the lowest adjustable g-force (100 x g) is
displayed upon switching. The exact g-force (rcf) can be determined using the formula
given below.
Ensure that the g-force (rcf) displayed upon switching is standardized to suit the rotor
without an adapter. You can achieve the following maximum g-forces (rcf) at maximum
speed (rpm) when adapters are used:
Adapter
Max. centrifugation
radius rmax [cm]
Max. g-force (rcf)
Without adapter
7.7
16,873
for 0.2 mL PCR tubes
5.6
12,271
for 0.4 mL micro test tubes
7.7
16,873
for 0.5 mL micro test tubes
6.6
14,462
for 0.6 mL Microtainers
7.7
16,873
To determine the g-force (rcf) for a special adapter, you can perform a calculation according to DIN 58 970
with the following formula:
rcf = 1.118 10-5 n2 rmax
n: Revolutions per minute (rpm)
rmax: Max. centrifugation radius in cm
Example:
The 0.2 ml adapter has a maximum radius of 5.6 cm. At 5,000 rpm a maximum g-force of 1,565 x g is
reached.
Safety
English (EN)
Safety
3.1
Intended use
The Centrifuge 5418 / 5418 R is intended exclusively for indoor use and for separating aqueous solutions
and suspensions of various densities in approved test tubes.
3.2
User profile
This device may only be operated by trained specialist staff. They must have carefully read the operating
manual and be familiar with the function of the device.
3.3
Application limits
3.3.1
Declaration concerning the ATEX directive (94/9/EC)

DANGER! Risk of explosion.
Do not operate the device in areas where work is completed with explosive substances.
Do not use this device to process any explosive or highly reactive substances.
Do not use this device for processing any substances which could generate an explosive
atmosphere.
Due to its design and the environmental conditions inside the device, the Centrifuge 5418 / 5418 R is not
suitable for use in a potentially explosive atmosphere.
The device only must be used in a safe environment, such as the open environment of a ventilated
laboratory or fume hood. The use of substances which could create a potentially explosive atmosphere is
not permitted. The final decision on the risks associated with the use of these types of substances is the
responsibility of the user.
3.3.2
Maximum service life for accessories

WARNING! Risk of injury from chemically or mechanically damaged accessories.
Even minor scratches and cracks can lead to serious internal material amage.
Protect all accessory parts from mechanical damage.

Inspect the accessories for damage before each use. Replace any damaged accessories.
Do not use rotors or rotor lids with signs of corrosion or mechanical damage (e.g.
deformations).
Do not use any accessories which are past their use-by date.
11
12
Safety
English (EN)
Accessories
Maximum service from the first

commissioning onward
Rotor lid
3 years
Requirement: The "click" is still
audible when tightening the rotor lid
screw.
Plastic adapters
1 year
For the rotor described there is no limit on the service life as long as the following prerequisites are met:
Proper use,
Recommended maintenance
Undamaged condition
The date of manufacture is stamped on the rotors in the format 03/10 (= March 2010). This is for
information only and does not have any reference to the service life.
To ensure aerosol tightness, the following applies:
Replace aerosol-tight rotor lids and caps after 50 autoclaving cycles.
Replace the seal of QuickLock rotor lids after 50 autoclaving cycles.
3.4
Information on product liability
In the following cases, the designated protection of the device may be compromised. Liability for any
resulting property damage or personal injury is then transferred to the operator:
The device is not used in accordance with the operating manual.

The device is used outside of its intended use.
The device is used with accessories or consumables which are not recommended by Eppendorf.
The device is maintained or repaired by people not authorized by Eppendorf.
The user makes unauthorized changes to the device.
3.5
Warnings for intended use
Read the operating manual and observe the following general safety instructions before using the
Centrifuge 5418 / 5418 R.
3.5.1
Personal injury or damage to the equipment

WARNING! Electric shock due to damage to device or mains cable.
Only switch on the device if the device and mains cable are undamaged.
Only use devices that have been properly installed or repaired.
In case of danger, disconnect the device from the mains supply by pulling the power plug
from the device or the mains socket or, by using the isolating device intended for this
purpose (e.g., emergency stop switch in the laboratory).
Safety
English (EN)
WARNING! Lethal voltages inside the device.
Ensure that the housing is always closed and undamaged so that no parts inside the device
can be contacted by accident.
Do not remove the housing of the device.

Do not allow any liquids to penetrate the inside of the housing.
Do not allow the device to be opened by anyone except service personnel who have been
specifically authorized by Eppendorf.
WARNING! Risk from incorrect supply voltage
Only connect the device to voltage sources that match the electrical requirements listed on
the name plate.
Only use sockets with a protective earth (PE) conductor and suitable power cable.
WARNING! Damages to health due to infectious liquids and pathogenic germs.
When handling infectious liquids and pathogenic germs, observe the national regulations,
the biological security level of your laboratory, the material safety data sheets, and the
manufacturer's application notes.
Use aerosol tight sealing systems for the centrifugation of these substances.
When working with pathogenic germs belonging to a higher risk group, more than one
aerosol-tight bioseal must be used.
Wear personal protective equipment.
Consult the "Laboratory Biosafety Manual" (Source: World Health Organization,
Laboratory Biosafety Manual, as amended) for comprehensive regulations on the handling
of risk group II germs or biological materials).
WARNING! Crushing of the fingers with the centrifuge lid.
When opening or closing the device lid, do not reach between the lid and device or into the
latching mechanism of the lid.
Always open the centrifuge lid completely to prevent it from falling.

CAUTION! Poor safety due to incorrect accessories and spare parts.
The use of accessories and spare parts other than those recommended by Eppendorf may
impair the safety, functioning and precision of the device. Eppendorf cannot be held liable or
accept any liability for damage resulting from the use of incorrect or non-recommended
accessories and spare parts, or from the improper use of such equipment.
Only use accessories and original spare parts recommended by Eppendorf.
13
14
Safety
English (EN)
NOTICE! Damage to device due to spilled liquids.

1. Switch the device off.
2. Disconnect the device from the power supply.
3. Carefully clean the device and the accessories in accordance with the cleaning and
disinfection instructions in the operating manual.
4. If a different cleaning and disinfecting method is to be used, contact Eppendorf AG to
ensure that the intended method will not damage the device.
NOTICE! Damage to electronic components due to condensation.
Condensation can form inside the device after the device has been moved from a cool to a
warmer environment.
5418: Wait for at least 3 hours before connecting the device to the power supply.
Only 5418: Alternative: Let the device heat up for 30 minutes right before a brief transport.
5418 R: Wait for at least 4 hours before connecting the device to the power supply.
3.5.2
Incorrect handling of the centrifuge

NOTICE! Damage from knocking against or moving the device during operation.
If the rotor bangs against the rotor chamber wall, it will cause considerable damage to the
device and rotor.
Do not move or knock against the device during operation.
3.5.3
Incorrect handling of the rotors

WARNING! Risk of injury from improperly attached rotors and rotor lids.
Only centrifuge with rotor and rotor lid firmly tightened.

If unusual noises occur when the centrifuge starts, the rotor or the rotor lid may not be
properly secured. Immediately press the start/stop key to stop centrifuging.
CAUTION! Risk of injury due to asymmetric loading of a rotor.
Load rotors symmetrically with identical tubes.

Only load adapters with suitable tubes.
Always use the same type of tubes (weight, material/density and volume).
Check symmetric loading by balancing the adapters and tubes used with scales.
CAUTION! Risk of injury from overloaded rotor.

The Centrifuge 5418 / 5418 R is designed for the centrifugation of centrifugation material with
a max. density of 1.2 g/mL at maximum speed and volume.
Please note the information on each rotor on the maximum load (adapter, tube and
contents) per rotor bore and do not exceed it.
Safety
English (EN)
NOTICE! Damage to rotors from aggressive chemicals.

Rotors are high-quality components which withstand extreme stresses. This stability can be
impaired by aggressive chemicals.
Avoid the use of aggressive chemicals, including strong and weak alkali, strong acids,
solutions with mercury, copper and other heavy metal ions, halogenated hydrocarbons,
concentrated saline solutions and phenol.
Due to the manufacturing process, color variations may occur on rotors marked "coated".
These color variations do not effect service life or resistance to chemicals.
3.5.4
Extreme strain on the centrifuging tubes

CAUTION! Risk of injury from overloaded tubes.
Note the loading limits specified by the tube manufacturer.

Only use tubes which are approved by the manufacturer for the required rcf.
NOTICE! Risk from damaged tubes.
Damaged tubes must not be used, as this could cause further damage to the device and the
accessories and loss of the samples.
Before use, visually check all of the tubes for damage.

NOTICE! Risk from open tube lids.
Open tube lids can break off during centrifugation and damage both the rotor and the
centrifuge.
Carefully seal all tube lids before centrifuging.

NOTICE! Hazard to plastic tubes from organic solvents.
The density of plastic tubes is reduced when organic solvents (e.g., phenol, chloroform) are
used, i.e. the tubes could become damaged.
Note the manufacturers information on the chemical resistance of the tubes.

NOTICE! Sample tubes heat up.
In uncooled centrifuges, the temperature in the rotor chamber, rotor and sample can increase
to above 40 C, based on the run time, g-force (rcf)/speed and ambient temperature.
Note that this can reduce the centrifugation resistance of the sample tubes.
Please note the temperature resistance of the samples.
15
16
Safety
English (EN)
3.5.5
Aerosol-tight centrifugation
WARNING! Risk to health due to limited aerosol tightness with incorrect rotor/rotor lid
combination.
Aerosol-tight centrifugation is guaranteed only if the rotors and rotor lids intended for this
purpose are used. For fixed-angle rotors the labeling always begins with FA, swing-bucket
rotors are labeled with AT (aerosol tight).
The aerosol-tight rotors and rotor lids of this centrifuge are additionally marked with a red
ring on the rotor and a red rotor lid screw.
For aerosol-tight centrifugation, always simultaneously use rotors and rotor lids which are
marked as aerosol-tight in the centrifuge intended for the corresponding purpose. The
details specifying in which centrifuge you may use the aerosol-tight rotors and rotor lids
can be found on the rotor and, beginning from production date of October 2003, on the
upper side of the rotor lid.
Only use aerosol-tight rotor lids in combination with rotors which are marked on the rotor
lid.
WARNING! Health hazard from limited aerosol-tightness due to incorrect use.
Autoclaving, mechanical stresses and contamination by chemicals or other aggressive
solvents can impair the aerosol-tightness of the rotors and rotor lid.
Check the integrity of the seals of the aerosol-tight rotor lids or caps before each use.
Only use aerosol-tight rotor lids or caps if the seals are undamaged and clean.
Thinly brush the threads of the rotor lid screw with pivot grease (order no. Int.
5810 350.050, North America 022634330). Do not apply the pivot grease to the seals.
Replace aerosol-tight rotor lids and caps after 50autoclaving cycles.

For QuickLock rotor lids, the seal must be replaced after 50 autoclaving cycles.
Never store aerosol-tight rotors or buckets closed.
3.6
Safety instructions located on the device

Display
Meaning
Location
Follow the instructions in the operating manual.
5418: Rear of the device

5418 R: Right side of the
device
CAUTION
Top of device, below the
Always tighten the rotor using the supplied rotor centrifuge lid
key.
CAUTION
Close all tubes and use a rotor lid.
Top of device, below the

centrifuge lid
Installation
English (EN)
Installation
4.1
Selecting the location

NOTICE! If an error occurs, the objects in the immediate proximity of the device will be
damaged.
In accordance with recommendations in EN 61010-2-020, leave a safety clearance of 30

cm around the device during operation.
Please remove all materials and objects from this area.

NOTICE! Damage from overheating.
Do not install the device near to any heat sources (e.g., heating, drying cabinet).
Do not expose the device to direct sunlight.
Ensure unobstructed air circulation. Keep free a clearance of at least 33 cm around all
ventilation grilles.
Select the location for the device according to the following criteria:
Suitable power connection as per the name plate (230 V/120 V/100 V).
Stable, horizontal and resonance-free lab bench. Weight of the device: 7.7 kg (5418) or 22 kg (5418 R).
A well ventilated environment which is protected from direct sunlight to prevent the device from heating
up more.
4.2
Preparing installation
Prerequisites
The weight of the Centrifuge 5418 is 7.7 kg or the Centrifuge 5418 R 22 kg. For unpacking and installing
the Centrifuge 5418 R, you require the assistance of another person.
Perform the following steps in the sequence described.
1. Open the packaging board.
2. Remove the accessories.
3. Lift the Centrifuge 5418 / 5418 R out of the carton. Lift the Centrifuge 5418 R out of the carton with the
help of another person.
4. Remove the front and back transport protection pads.
5. Place the device on a suitable lab bench.
6. Remove the plastic sleeve.
17
18
Installation
English (EN)
4.3
Installing the instrument

NOTICE! Damage to electronic components due to condensation.
Condensation can form inside the device after the device has been moved from a cool to a
warmer environment.
5418: Wait for at least 3 hours before connecting the device to the power supply.
Only 5418: Alternative: Let the device heat up for 30 minutes right before a brief transport.
5418 R: Wait for at least 4 hours before connecting the device to the power supply.
NOTICE! Centrifuge 5418 R: compressor damage after improper transport.
Only switch on the centrifuge 4 hours after installation.

1. Let the device warm up to ambient temperature.
2. Check that the mains voltage and frequency match the requirements on the device type plate.
3. Connect the centrifuge to the mains and switch it on using the mains/power switch.
Display is active.
Lid opens automatically
4. Loosen and remove the rotor using the supplied rotor key.
5. Remove the transport protection pad from the motor shaft.
6. Use the details included in the scope of delivery to check that the delivery is complete.
7. Check all parts for any transport damage. Contact your dealer if any damage is found.
8. Only 5418 R: Insert the condensation water tray at the side of the device into the holder provided.
Retain the packaging material and the transport protection device for subsequent transport or
storage. See also the instructions relating to transport.
Operation
English (EN)
Operation
5.1
Overview of operating controls
Familiarize yourself with the operating controls and the display before using the Centrifuge 5418 / 5418 R
for the first time.
Abb. 5-1: Control panel of Centrifuge 5418 and Centrifuge 5418 R.
5418
5 (1)
5418 R
4
Fig. 5-1:
4 (9)
Control panel of Centrifuge 5418 and Centrifuge 5418 R.
1 Switch the displayed speed of centrifugation

(rpm/rcf)
6 Adjust temperature (only 5418 R)
2 Display
7 Start the temperature control run fast temp

(only 5418 R)
3 Release lid
8 Short-spin centrifugation
4 Set centrifugation time

9 Start and stop centrifugation
Only 5418 R: Press the time dial to start and stop
centrifugation.
5 Set speed of centrifugation
Only 5418 R: Press the speed dial to switch the
displayed centrifugation speed (rpm/rcf).
19
20
Operation
English (EN)
Abb. 5-2: Display of Centrifuge 5418 and Centrifuge 5418 R.
5418
Fig. 5-2:
5418 R
Display of Centrifuge 5418 and Centrifuge 5418 R.
1 Centrifugation time
5 Temperature (only 5418 R)
2 g-force (rcf) / speed (rpm)

5418 R: Set value x 1000
6 Status of the key lock (only 5418 R)

: Centrifuging parameters cannot be modified
unintentionally.
: No key lock.
3 Status of centrifuge
: Centrifuge lid unlocked.
: Centrifuge lid locked.
(Flashing): Centrifuging in progress.
7 Status of centrifugation speed display

rcf: g-force (relative centrifugal force, RCF)
rpm: speed (revolutions per minute)
4 At set rpm (only 5418 R)

: Start of run time when reaching 95% of the 8 Status of the loudspeaker (only 5418 R)
preset g-force (rcf) / speed (rpm).
: Switched on.
: Immediate start of run time.
No symbol: Switched off.
5.2
Preparing for centrifugation
5.2.1
Switching on the centrifuge
1. Switch on the centrifuge using the mains switch.

The centrifuge lid opens automatically after switching on using the mains switch.
2. Open the closed centrifuge lid by pressing the open key.
The parameter settings of the last run are displayed.
5.2.2
Inserting the rotor

NOTICE! If handled incorrectly, the rotor can fall over.
The rotor lid screw may become loose if it is used to retain the rotor.
Always grasp the rotor with both hands for holding or transport.
1. Place the rotor on the motor shaft.

2. Insert the delivered rotor key in the rotor nut.
3. Turn rotor key clockwise until the rotor nut is firmly tightened.
Operation
English (EN)
5.2.3
Loading the rotor

CAUTION! Risk of injury due to asymmetric loading of a rotor.
Load rotors symmetrically with identical tubes.

Only load adapters with suitable tubes.
Always use the same type of tubes (weight, material/density and volume).
Check symmetric loading by balancing the adapters and tubes used with scales.
CAUTION! Risk from damaged or overloaded tubes.
When loading the rotor, observe the safety precautions on dangers as a result of
overloaded or damaged tubes (see Warnings for intended use on p. 12).
To load the rotor, proceed as follows:

1. Check the maximum load (adapter, tube and contents) per rotor bore.
The maximum load is 3.75 g per rotor bore. This information can also be found on the rotor.
2. Load rotors and adapters only with the tubes intended for them.
3. Insert tubes opposite each other in pairs into the rotor bores. For symmetric loading, tubes that face
each other must be the same type and have the same filling quantity.
To keep the weight differences between the filled tubes low, we recommend taring with a balance. This
is gentle on the drive and reduces the operating noises.
5.2.4
Closing the rotor lid

With the rotor FA-45-18-11 centrifugation is also possible without a rotor lid.
Please also note:
The tube lids must be closed securely.
Without the rotor lid, the rotor is not aerosol-tight.
The centrifugation is slightly louder.
Spin columns must always be centrifuged with a rotor lid.
21
22
Operation
English (EN)
1. Check to see if the outer sealing ring is correctly positioned in the

groove.
2. Fit the rotor lid vertically onto the rotor.
3. Lock the rotor by turning the red rotor lid screw clockwise beyond
an audible click until it can be turned no further.
The rotor is correctly locked after the audible "click" is heard!
5.2.5
Closing the centrifuge lid

WARNING! Crushing of the fingers with the centrifuge lid.
When opening or closing the device lid, do not reach between the lid and device or into the
latching mechanism of the lid.
Always open the centrifuge lid completely to prevent it from falling.
1. Check correct attachment of rotor and rotor lid.

2. Push down the centrifuge lid until the lid latch engages and the lid is automatically closed.
The centrifuge will close automatically.
The display shows the
symbol.
Only 5418: The open key lights blue.
5.3
Cooling (only 5418 R)
5.3.1
Temperature adjustment
Set the temperature using the temp arrow keys from 0C to +40C.
5.3.2
Temperature display
If the rotor is stopped:

During centrifugation:
5.3.3
Set temperature
Actual temperature
Temperature monitoring
After the set temperature has been reached the centrifuge responds as follows to temperature fluctuations
during centrifugation:
Deviation from the target value
Action
T > 3C
Temperature display flashes.
T > 5C
Periodic warning tone and display Error 18.

Centrifugation is stopped automatically.
Operation
English (EN)
5.3.4
FastTemp
This function can be used to start a temperature control run directly without samples with a rotor and
temperature-specific speed in order to quickly adjust the rotor chamber, incl. rotor and adapters, up to the
set target temperature.
Prerequisites
The centrifuge is switched on.

Rotor and rotor lid are correctly mounted.
The centrifuge lid is closed.
The temperature and g-force (rcf) or speed (rpm) are set for the subsequent centrifugation (see
Centrifuging on p. 24).
1. Press the fast temp key.

The display shows FT as well as the current temperature and g-force (rcf) or speed (rpm).
The cooling time from room temperature (23 C) to 4 C takes Approx. 16 min.
The temperature control cycle ends automatically when the target temperature has been reached. A
periodic signal tone sounds.
2. Press the start/stop key to end the temperature control run early.
After the set temperature has been reached and the temperature control run is complete, the centrifuge
keeps the rotor chamber, with the centrifuge lid closed, at the set temperature, if the temperature is below
the ambient temperature. Irrespective of the target temperature, however, this continuous cooling does not
go below 4C to prevent the rotor chamber from freezing.
The centrifuge stops the run automatically when the rotor has reached the set temperature.
Therefore, there may be a delay between the display of the achieved set temperature and the
automatic end of the temperature control run.
5.3.5
Continuous cooling
When the rotor is stopped the rotor chamber is kept at the target temperature when the following
prerequisites are met:
The centrifuge is switched on.
The centrifuge lid is closed.
The target temperature is below the ambient temperature.
During continuous cooling the following applies:
The target temperature is displayed.
Irrespective of the target temperature, continuous cooling does not go below 4C to prevent the rotor
chamber and the samples from freezing and increased condensation in the device.
The temperature adjustment takes longer because the rotor is not rotating.
Open the centrifuge lid to end continuous cooling.
If the centrifuge is not used for more than 8 hours, the continuous cooling is switched off automatically.
This protects against ice formation in the rotor chamber and the tubes, and against increased condensation
in the device.
The display shows the actual temperature of the rotor chamber.
With fast temp you can quickly reach the desired temperature again (see p. 23).
23
24
Operation
English (EN)
5.4
Centrifuging
CAUTION! Risk from incorrectly-loaded rotors and damaged/overloaded tubes!
Before commencing centrifugation, follow the safety instructions relating to risks from
asymmetrically loaded and/or overloaded rotors and from overloaded, damaged and/or
open tubes (see Warnings for intended use on p. 12).
WARNING! Risk of injury from improperly attached rotors and rotor lids.
Only centrifuge with rotor and rotor lid firmly tightened.

If unusual noises occur when the centrifuge starts, the rotor or the rotor lid may not be
properly secured. Immediately press the start/stop key to stop centrifuging.
Before using the Centrifuge 5418 / 5418 R for the first time, familiarize yourself with the operating controls
and the display (see Overview of operating controls on p. 19).
Each of the centrifuging variants described here must be preceded by the preparation described above (see
Preparing for centrifugation on p. 20).
Only 5418 R: Please also note the instructions on cooling (see p. 22).
5.4.1
Centrifugation with time setting

1. Use time to set the run time.
2. Only 5418 R: Use temp to set the temperature.
3. Use speed to set the g-force (rcf) or speed (rpm).
4. Press start/stop to start centrifuging.
During centrifugation:
In the display flashes while the rotor is running.
Only 5418 R: The current temperature will be displayed.
The fast temp (only 5418 R), open and short keys are blocked during centrifugation.
During the run you can modify the total run time, the temperature (only 5418 R), the speed and the
rpm/rcf display. Only 5418 R: To change the centrifugation parameters, press the short key first.
The values flash in the display during the change. The new parameters are adopted immediately.
When the time is changed during a run, the time which has already elapsed is taken into account.
Please note that the shortest new total run time that can be set is the elapsed time plus 2 minutes.
You can also terminate the centrifugation before the set run time has elapsed by pressing the start/
stop key.
Operation
English (EN)
End of centrifugation
The centrifuge automatically stops after the set time has elapsed. The elapsed centrifugation will be
shown in a blinking display during the braking process. A signal tone sounds when the rotor is at a
standstill.
Only 5418: The centrifuge lid opens automatically. The display shows the symbol .
Only 5418 R: The centrifuge lid remains closed to maintain the sample temperature. You can open it
by pressing the open key.
5. Remove the material for centrifuging.
5.4.2
Centrifuging in continuous operation

1. Use time to set the continuous run.
The continuous run function can be set above 9:59 h or under 30 s (5418) or above 99 min or under
0.5 min (5418 R). The timer shows oo to indicate continuous operation.
2. Only 5418 R: Use the temp arrow keys to adjust the temperature.
3. Use speed to set the g-force (rcf) or speed (rpm).
4. Press start/stop to start centrifuging.
Time is counted upwards, first in 30-second increments and then in minute increments from ten
minutes.
5. Press start/stop to end centrifuging after the desired time period.
The centrifugation time will be shown in a blinking display during the braking process.
A signal tone sounds when the rotor is at a standstill.
5.4.3
Short-spin centrifugation
5418: You can carry out a short run with the maximum g-force (rcf) / speed (rpm).
5418 R: You can carry out a short run with the currently set or with the maximum g-force (rcf) /
speed (rpm). Set this short spin mode as described in the following section.
5.4.3.1 Selecting short spin mode (only 5418 R)

1. Press the short key while the centrifuge lid is open.
The current mode is displayed:
Display 1 14 t (run at preselected speed)
Display 14 t (run at maximum speed of 14.000 rpm)
2. When the centrifuge lid is open, press the short key for longer than 2 s to switch between these modes.
25
26
Operation
English (EN)
5.4.3.2 Performing short spin centrifugation

1. Only 5418 R: A short run at preselected g-force (rcf) or speed (rpm) can be set directly using the speed
dial.
2. Only 5418 R: Use the temp arrow keys to adjust the temperature.
3. Start short run: Hold down the short key.
The time is counted upwards in seconds.
During short run centrifuging all other keys are blocked.
4. End short run: Release the short key.
The centrifugation time will be shown in a blinking display during the braking process.
During the braking process, you can restart the centrifugation up to two times by pressing the
short key again.
5.4.4
Removing the rotor
1. Turn the rotor nut counterclockwise using the supplied rotor key.
2. Remove the rotor vertically in an upward motion.
3. Only 5418 R: Switch off the centrifuge after use and empty the condensation water tray (pull out from
the left or right side of the device). Leave the centrifuge lid fully open and secure it against closing.
5.5
Standby mode (only 5418)
If the centrifuge has not been used for 15 min, it switches to standby mode. The EP logo then appears in
the display. When a button or knob is used or the centrifuge lid is closed, the centrifuge is reactivated and
ready for operation.
5.6
Special functions (only 5418 R)
Function
Status of centrifuge lid
Press > 2 s key
Display
Flashes 5 s
Modify parameter during

the cycle.
closed
short key
Enable/disable signal
tone.
open
open
Enable/disable key lock.
open
short + open
At set rpm
open
time
/
/
Maintenance
English (EN)
Maintenance
6.1
Prepare cleaning/disinfection
The procedure described in the following chapter applies to the cleaning as well as to the disinfection or
decontamination. The table below describes the steps required on top of this:
Cleaning
Disinfecting/decontamination
1. Use a mild cleaning fluid to clean the accessible 1. Choose the disinfection method which
surfaces of the device and the accessories.
corresponds to the legal regulations and
2. Carry out the cleaning as described in the
guidelines in place for your range of application.
following chapter.
For example, use alcohol (ethanol, isopropanol)
or alcohol-based disinfectants.
2. Carry out the disinfection or decontamination as
described in the following chapter.
3. Then clean the device and the accessories.
If you have any further questions regarding the cleaning and disinfection or decontamination
or regarding the cleaning fluid to be used, contact the Eppendorf AG Application Support. The
contact details are provided on the back of this manual.
6.2
Cleaning/disinfection
DANGER! Electric shock as a result of penetration of liquid.
Switch off the device and disconnect the power plug before starting cleaning or
disinfection work.
Do not allow any liquids to penetrate the inside of the housing.

Do not spray clean/spray disinfect the housing.
Only plug the device back in if it is completely dry, both inside and outside.
NOTICE! Damage from the use of aggressive chemicals.
Do not use any aggressive chemicals on the device or its accessories, such as strong and
weak bases, strong acids, acetone, formaldehyde, halogenated hydrocarbons or phenol.
If the device has been contaminated by aggressive chemicals, immediately clean it by

means of a mild cleaning agent.
NOTICE! Corrosion from aggressive cleaning agents and disinfectants.
Do not use corrosive cleaning agents, aggressive solvents or abrasive polishes.

Do not incubate the accessories in aggressive cleaning agents or disinfectants for a longer
period of time.
27
28
Maintenance
English (EN)
NOTICE! Damage from UV and other high-energy radiation.
Do not use UV, beta, gamma, or any other high-energy radiation for disinfecting.
Avoid storage in areas with strong UV radiation
Autoclaving
Rotors, rotor lids and adapters can be autoclaved (121 C, 20 min).
Replace the sealing ring in the lid groove of the aerosol-tight rotor lid after a maximum of 50
autoclaving cycles.
Aerosol tightness
Check that the seals are intact before use.
Only QuickLock rotor lid: Replace the sealing ring in the lid groove when it becomes worn.
The sealing rings require regular care to protect the rotors.
Aerosol-tight rotors should never be stored with lids screwed on!
In order to prevent damage, lightly lubricate the lid thread of the aerosol-tight rotors with
pivot grease (order no. Int.: 5810 350.050/North America: 022634330).
6.2.1
Cleaning and disinfecting the device
1. Open the lid. Switch off the device with the mains/power switch. Disconnect the power plug from the
power supply.
2. Loosen the rotor nut by turning the rotor key counterclockwise.
3. Remove the rotor.
4. Clean and disinfect all accessible surfaces of the device, including the power cable, using a damp cloth
and the recommended cleaning agents.
5. Thoroughly clean the rubber seals of the rotor chamber with water.
6. Rub the dry rubber seals with glycerine or talcum powder to prevent them from becoming brittle. Other
components of the device, such as the lid latch, motor shaft and rotor cone, must not be lubricated.
7. Clean the motor shaft with a soft, dry and lint-free cloth. Do not lubricate the motor shaft.
8. Check the motor shaft for damage.
9. Inspect the device for corrosion and damage.
10. Leave the centrifuge lid open when the device is not being used.
11. Only connect the device to the power supply if it is fully dry inside and out.
6.2.2
Cleaning and disinfecting the rotor
1. Inspect the rotor and accessories for damage and corrosion. Do not use any damaged rotors or
accessories.
2. Clean and disinfect the rotors and accessories with the recommended cleaning agents.
3. Use a bottle brush to clean and disinfect the rotor bores.
4. Remove the sealing ring to clean and disinfect the rotor lid. Clean the groove below it and the sealing
ring.
Maintenance
English (EN)
5. Rinse the rotors and accessories thoroughly with distilled water. Rinse the rotor bores of fixed-angle
rotors particularly thoroughly.
Do not immerse the rotor in liquid as liquid can get trapped inside the cavities.
6. Place rotors and accessories on a cloth to dry. Place fixed-angle rotors with the rotor bores facing
downwards to allow the bores to also dry.
7. Correctly reinsert the rotor lid sealing ring in the clean and dry groove.
8. Clean the rotor cone with a soft, dry and lint-free cloth. Do not lubricate the rotor cone.
9. Inspect the rotor cone for damage.
10. Place the dry rotor onto the motor shaft.
11. Tighten the rotor nut firmly by turning it clockwise with the rotor key.
12. Leave the rotor lid open when the rotor is not being used.
6.3
Additional service instructions for Centrifuge 5418 R
Empty and clean the condensation water tray regularly and especially after liquid spillage in the rotor
chamber. Pull out the condensation water tray from the left or right side of the centrifuge.
Regularly free the rotor chamber ice formations via thawing, by leaving the centrifuge lid open or
carrying out a short temperature control run at approx. 30 C.
Leave the centrifuge lid open when not in use for a long period.
Wipe up condensate in the rotor chamber using a soft, absorbent cloth.
Remove dust deposits from the ventilation slits of the centrifuge using a brush or swab at the latest
every six months. First switch off the device and remove the power plug.
6.4
Glass breakage
When using glass tubes there is a risk of glass breakage in the rotor chamber. The resulting glass splinters
are swirled around in the rotor chamber during centrifugation and have a sandblasting effect on the rotor
and accessories. The smallest glass particles become lodged in the rubber parts (e.g., the motor guide, the
rotor chamber gasket, and the rubber mats of adapters).
29
30
Maintenance
English (EN)
NOTICE! Glass breakage in the rotor chamber

Glass tubes in the rotor chamber may break if the g-force is too high. Broken glass can
damage the rotor, accessories and samples.
Please note the manufacturer's information on the recommended centrifugation

parameters (load and speed).
Effects of glass breakage in the rotor chamber:
Fine black metal abrasion in the rotor chamber (in metal rotor chambers)
The surfaces of the rotor chamber and accessories are scratched.
The chemical resistance of the rotor chamber is reduced.
Contamination of samples
Wear on rubber parts
How to proceed in case of glass breakage
1. Remove all splinters and glass powder from the rotor chamber and accessories.
2. Thoroughly clean the rotor and rotor chamber. Thoroughly clean the bores of the fixed-angle rotors, in
particular.
3. If required, replace the adapters to prevent any further damage.
4. Regularly check the rotor bores for deposits and damage.
6.5
Fuses
The fuse holder is located under the mains power socket (5418) or on the left next to the mains switch
(5418 R).
1. Disconnect the mains plug.
2. Remove the fuse holder.
Both fuses are now accessible and can be replaced.
6.6
Decontamination before shipment
If you are shipping the device to the authorized Technical Service for repairs or to your authorized dealer
for disposal please note the following:
WARNING! Risk to health from contaminated device
1. Observe the notes on the decontamination certificate. You find it as a PDF file on our
website (www.eppendorf.com/decontamination).
2. Decontaminate all the parts you would like to dispatch.
3. Include the fully completed decontamination certificate in the package.
Troubleshooting
English (EN)
Troubleshooting
If you cannot remedy an error with the recommended measures, please contact your local Eppendorf
partner. The contact address can be found online at: www.eppendorf.com/worldwide.
7.1
General errors
Symptom/message Cause
Remedy
No display.
No power connection.
Check mains/power connection.
No display.
Power failure.
Check the mains fuse of the device

(see Fuses on p. 30).
Check the mains fuse of the

laboratory.
Lid of the device
cannot be opened.
Rotor is still running.
Wait for rotor to stop.
Lid of the device

cannot be opened.
Power failure.
1. Check the mains fuse of the device

(see Fuses on p. 30).
2. Check the mains fuse of the
laboratory.
3. Activate the emergency lid release
(see p. 33).
Device cannot be
started.
Lid of the device is not closed.
Close device lid.
Device shakes
when it starts up.
Rotor loaded unsymmetrically.
1. Stop the device and load

symmetrically.
2. Restart device.
Centrifuge brakes
during a short run
centrifugation,
although the short
key is pressed.
The short key was released briefly more

than twice (protective function for the
drive).
Press the short key continuously
Temperature
display flashes.
(only for 5418 R)
Temperature deviation from the set

value: 3 C.
Check the settings.

Wait until the set temperature has
during a short run centrifugation.
been reached.
Check unhindered air circulation

through the air slots.
Thaw ice or switch off device and
allow it to cool down.
31
32
Troubleshooting
English (EN)
7.2
Error messages
If one of the following error messages appears, proceed as follows:

1. Remove fault (see Remedies).
2. If necessary, repeat centrifugation.
Remedy
LID ERROR (5418)/ Centrifuge lid cannot be locked.

LID (5418 R)
Try again to close centrifuge lid.
LID ERROR (5418)/ Centrifuge lid cannot be released.

LID (5418 R)
1. Switch the centrifuge off and back on.

2. Press the open key.
If the error occurs again:
1. Switch off centrifuge.
2. Activate the emergency lid release
(see Emergency lid release on p. 33).
LID ERROR (5418)/ Centrifuge lid must not be released

LID (5418 R)
during a run.
Wait for rotor to stop.
INT
Mains/power failure during a run.
Check the mains connection.
NO RPM (5418)/
Error 3 (5418 R)
Error in the speed measuring system or

drive overheated.
Leave the device switched on until the
Error 5
(only for 5418 R)
Prohibited opening of lid or lid switch is

defective during a run.
1. Wait for rotor to stop.

2. Open and close again the lid of the
device.
3. Repeat the run.
Err 6 (5418)/
Error 6 (5418 R)
Drive fault.
Repeat the run.

If this error message appears again,
error message disappears (10 s or 6

min).
switch centrifuge off and back on

again after > 20 s.
Allow the drive to cool down for at
Error 6
(only for 5418 R)
Drive overheated.
Err 7 (5418)/
Error 7 (5418 R)
Major deviation in the speed control.

2. Tighten rotor.
Err 8 (5418)/
Error 8 (5418 R)
Drive fault.

2. Repeat the run.
least 15 min.
Err 9 to 17 (5418)/ Electronics error.

Error 9 to 17 (5418
R)
Switch centrifuge off and back on
Error 18
(only for 5418 R)
Check the settings.

Too high temperature deviation from set

value in the rotor chamber.
again after > 20 s.
through the air slots.
Thaw ice or switch off device and

allow it to cool down.
Troubleshooting
English (EN)
Remedy
Error 19
(only for 5418 R
Cooling circuit is overheated.
Error 20
(only for 5418 R)
Temperature sensor in rotor chamber is

faulty.
Error 21
(only for 5418 R)
Temperature sensor on condenser is

faulty.
Error 24
(only for 5418 R)
Cooling unit fault, e.g. overheated.
Allow the centrifuge to cool down and
7.3
through the air slots and allow device

to cool down.
again after > 20 s.
again after > 20 s.
repeat the run.
Emergency lid release
If the centrifuge lid cannot be opened, the emergency lid release can be operated manually.
WARNING! Risk of injury from rotating rotor.
Wait until the rotor has come to a standstill before you operate the emergency release.
To check, look through the inspection glass in the centrifuge lid.
7.3.1
Centrifuge 5418

2. Open the plastic cover of the emergency lid release.
This is located behind the front left device foot in the base plate (Fig. 2-1 on p. 7).
3. Pull the cord vertically downwards.
The centrifuge lid is unlocked.
4. Before closing the centrifuge lid again: push the cord completely into the housing and insert the plastic
cover in the base plate.
7.3.2
Centrifuge 5418 R

2. Turn the plastic cover of the emergency lid release anti-clockwise by 90 with a suitable tool (e.g.
screwdriver), and remove it.
The plastic cover is located on the right side of the device (Fig. 2-2 on p. 7).
3. Insert the centrifuge rotor key into the hexagonal opening at rear until some resistance can be felt.
4. Press the rotor key slightly down, and turn it clockwise.
This will release the centrifuge lid.
5. Open the centrifuge lid.
6. Remove the rotor key and put the plastic cover back on rotating it 90 clockwise.
33
34
Troubleshooting
English (EN)
Transport, storage and disposal

English (EN)
8.1
Transport
CAUTION! Bodily injury due to lifting and carrying heavy loads
The device is heavy. Lifting and carrying the device can lead to back injuries.
The device must be transported by least two people.

Use a transport aid (e.g., dolly) to transport the device longer distances.
Remove the rotor from the centrifuge before transport.
Use the original packaging for transport.
Air temperature
Relative humidity
Atmospheric pressure
General transport
-25 C 60 C
10 % 75 %
30 kPa 106 kPa
Air freight
-20 C 55 C
10 % 75 %
30 kPa 106 kPa
Air temperature
Relative humidity
Atmospheric pressure
In transport packaging
-25 C 55 C
10 % 75 %
70 kPa 106 kPa
Without transport
packaging
-5 C 45 C
10 % 75 %
70 kPa 106 kPa
8.2
8.3
Storage
Disposal
In case the product is to be disposed of, the relevant legal regulations are to be observed.
Information on the disposal of electrical and electronic devices in the European Community:
Within the European Community, the disposal of electrical devices is regulated by national regulations
based on EU Directive 2002/96/EC pertaining to waste electrical and electronic equipment (WEEE).
According to these regulations, any devices supplied after August 13, 2005, in the business-to-business
sphere, to which this product is assigned, may no longer be disposed of in municipal or domestic waste. To
document this, they have been marked with the following identification:
Because disposal regulations may differ from one country to another within the EU, please contact your
supplier if necessary.
35
36

English (EN)
Technical data
English (EN)
Technical data
9.1
Power supply
Centrifuge 5418
Mains connection
230 V, 50 Hz 60 Hz
120 V, 50 Hz 60 Hz
100 V, 50 Hz 60 Hz
Current consumption
1.4 A (230 V )
2.8 A (120 V )
3.4 A (100 V )
Power consumption
Maximum 170 W
EMC: Interference emission. (radio interference)
EN 61326 Class B (230 V)

FCC15 - Class A (120 V/100 V)
EMC: Noise immunity
EN 61326
Overvoltage category
II
Fuses
2.5 AT (230 V)
5.0 AT (120 V / 100 V)
Degree of pollution
Centrifuge 5418 R
Mains connection
230 V, 50 Hz 60 Hz
120 V, 50 Hz 60 Hz
100 V, 50 Hz 60 Hz
Current consumption
1.4 A (230 V)
2.8 A (120 V)
3.0 A (100 V)
Power consumption
Maximum 320 W
EMC: Interference emission. (radio interference)
EN 61326 Class B (230 V)

FCC15 - Class A (120 V/100 V)
EMC: Noise immunity
EN 61326
Overvoltage category
II
Fuses
2.5 AT (230 V)
6.25 AT (120 V / 100 V)
Degree of pollution
37
38
Technical data
English (EN)
9.2
Ambient conditions
Environment:
Only for use indoors.
Ambient temperature:
Centrifuge 5418: 2 C to 40 C
Centrifuge 5418 R: 15 C to 35 C
Max. relative humidity:
75 %, non-condensing humidity.
Atmospheric pressure:
Use up to an altitude of 2 000 m above MSL.
Degree of pollution:
9.3
Weight/dimensions
Tab. 9-1: Centrifuge 5418

Dimensions:
Width: 208 cm (8.19 in)

Depth: 300 cm (11.8 in)
Height: 210 cm (8.27 in)
Weight without rotor:
7.7 kg
Noise level:
< 51 dB (A) *
Tab. 9-2: Centrifuge 5418 R

Dimensions:
Width: 298 cm (11.73 in)

Depth: 463 cm (18.23 in)
Height: 463 cm (18.23 in)
Weight without rotor:
22 kg
Noise level:
< 57 dB (A) *
*) The noise level was measured according to DIN EN ISO 3745 frontally in a sound measuring room with
accuracy class 1 at a distance of 1 m from the device and at lab bench height.
Technical data
English (EN)
9.4
Application parameters
Start and stop times according to DIN 58 970

Rotor
FA-45-18-11
5418
5418 R
Voltage
Acceleration
times
Deceleration
times
Voltage
Acceleration
times
Deceleration
times
230 V
120 V
100 V
16 s
16 s
18 s
18 s
18 s
18 s
230 V
120 V
100 V
13 s
13 s
13 s
13 s
13 s
13 s
These values were calculated at 23C.

run time
(5418)
30 s to 9:59 h, as well as infinity (oo),

Adjustable to 10 min in 30 s increments, then
increments of 1 min
run time
(5418 R)
0.5 min to 99 min, as well as infinity (oo),

Adjustable to 10 min in 0.5 min increments, then
increments of 1 min
Temperature
(only 5418 R)
0C to 40C
Relative centrifugal force (RCF)
1 to 16,873 x g
adjustable to 5000 g in 100 x g increments, then
increments of 200 g.
RPM
100 to 14,000 rpm

adjustable in 100 rpm increments
Maximum load
18 micro test tubes of 2.0 mL each.
Max. kinetic energy
2,600 Nm
Test log mandatory:
No
Allowable density of the centrifuge material (at

maximum g-force (rcf)/speed (rpm) and maximum
load)
1.2 g/mL
39
40
Technical data
English (EN)
Ordering information
English (EN)
10
10.1
Centrifuge 5418
Order no.
(International)
5418 000.017
-
10.2
022620321
022620304
Description
Centrifuge 5418
with rotor FA-45-18-11 incl. rotor lid
230 V / 50 60 Hz
120 V / 50 60 Hz, with US-plug
Centrifuge 5418 R
Order no.
(International)
5401 000.013
-
10.3
Order no. (North

America)
Order no. (North

America)
5401000013
5401000137
Description
Centrifuge 5418 R
with rotor FA-45-18-11 incl. rotor lid
230 V / 50 Hz
120 V / 50 60 Hz, with US-plug
Accessories
Order no.
(International)
Order no. (North

America)
5418 707.005
022652061
5418 708.001
022652087
5418 709.008
022652109
5425 715.005
5425 717.008
5425 716.001
022636260
022636243
022636227
5416 301.001
022634305
5401 850.076
5401850076
Description
Rotor FA-45-18-11
aerosol-tight, angle 45, 18 places, max. tube diameter 11 mm,
incl. rotor lid (aluminum)
Rotor lid for FA-45-18-11
aerosol-tight, aluminum
Seal for rotor lid FA-45-18-11 and FA-45-6-30
5 pieces
Adapter
used in FA-45-18-11
for 1 PCR tube (0.2 mL, max. 6 mm), set of 6
for 1 sample tube (0.4 mL, max. 6 mm), set of 6
for 1 sample tube (0.5 mL, max. 6 mm) or 1 Microtainer
(0.6 mL, max. 8 mm), set of 6
Rotor key
Standard
Tray for condensation water
*) Aerosol impermeability tested and certified by the Centre of Emergency Preparedness and Response,
Health Protection Agency, Porton Down (UK).
41
42
English (EN)
10.4
Fuses
10.4.1
Fuses for Centrifuge 5418
Order no.
(International)
Order no. (North

America)
5425 351.003
5425 353.006
022668188
022668226
10.4.2
Description
Fuse
2.5 A T (230 V), set of 2
5 A T (120 V), UL, set of 2
Fuses for Centrifuge 5418 R
Order no.
(International)
Order no. (North

America)
5425 351.003
5426 355.100
022668188
022668200
Description
Fuse
2.5 A T (230 V), set of 2
6.25 AT (100 V/120 V), set of 2
Evaluate your manual
Give us your feedback.

www.eppendorf.com/manualfeedback
Your local distributor: www.eppendorf.com/contact

Eppendorf AG 22331 Hamburg Germany
[email protected] www.eppendorf.com

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Centrifuge 5418 / 5418 R

Trademarks are not marked in all cases with or in this manual.

Cooling (only 5418 R) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

Using this manual

Danger symbols and danger levels

Will lead to severe injuries or death.

May lead to severe injuries or death.

May lead to light to moderate injuries.

May lead to material damage.

Actions in the specified order

Abb. 2-1: Front and rear view of the Centrifuge 5418

Front and rear view of the Centrifuge 5418

Abb. 2-2: Front and rear view of the Centrifuge 5418 R

Front and rear view of the Centrifuge 5418 R

Quantity Order no.

Quantity Order no.

Before using tubes, observe the manufacturer's recommended specifications on resistance to

18 micro test tubes of 1.5/

rcf display and calculation

Max. g-force (rcf)

for 0.2 mL PCR tubes

for 0.4 mL micro test tubes

for 0.5 mL micro test tubes

for 0.6 mL Microtainers

Declaration concerning the ATEX directive (94/9/EC)

Maximum service life for accessories

Protect all accessory parts from mechanical damage.

Maximum service from the first

Information on product liability

The device is not used in accordance with the operating manual.

Warnings for intended use

Personal injury or damage to the equipment

WARNING! Lethal voltages inside the device.

Do not remove the housing of the device.

WARNING! Crushing of the fingers with the centrifuge lid.

Always open the centrifuge lid completely to prevent it from falling.

Only use accessories and original spare parts recommended by Eppendorf.

NOTICE! Damage to device due to spilled liquids.

Incorrect handling of the centrifuge

Do not move or knock against the device during operation.

Incorrect handling of the rotors

Only centrifuge with rotor and rotor lid firmly tightened.

CAUTION! Risk of injury due to asymmetric loading of a rotor.

Load rotors symmetrically with identical tubes.

CAUTION! Risk of injury from overloaded rotor.

NOTICE! Damage to rotors from aggressive chemicals.

Extreme strain on the centrifuging tubes

Note the loading limits specified by the tube manufacturer.

Before use, visually check all of the tubes for damage.

Carefully seal all tube lids before centrifuging.

Note the manufacturers information on the chemical resistance of the tubes.

Replace aerosol-tight rotor lids and caps after 50autoclaving cycles.

Safety instructions located on the device

Follow the instructions in the operating manual.

5418: Rear of the device

Top of device, below the

Selecting the location

In accordance with recommendations in EN 61010-2-020, leave a safety clearance of 30