GENEzol DNA Reagent Plant

Catalogue Numbers
GR100
GR200

Quantity
100 ml
200 ml
CERTIFICATE NO. QAIC/TW/50077

Introduction

ISO 9001:2008 QMS

GENEzol DNA Reagent Plant provides a quick and easy 3 step CTAB and chloroform based method to isolate total DNA
(including genomic, mitochondrial and chloroplast DNA) from a variety of plant species (including algae and cyanobacteria).
This unique reagent is able to lyse most common plant samples and plant samples with high a polysaccharide content. The
extracted DNA is suitable for routine PCR screening, Real-Time PCR, Southern Blotting, Mapping and RFLP. Phenol
extraction is not required and the entire procedure can be completed within 50 minutes.

Quality Control

GENEzol DNA Reagent Plant is tested on a lot-to-lot basis according to Geneaids ISO-certified quality management
system. 50 mg of fresh Arabidopsis leaves are initially ground in GENEzol DNA Reagent Plant. A 15 l aliquot of extracted
genomic DNA from a 100 l eluate is analyzed by electrophoresis on a 1% agarose gel.

Advantages

High molecular weight genomic DNA extraction from a variety of plant species
Sample: up to 1 g of fresh plant tissue and up to 0.5 g of dry plant tissue
Scalable, simple and gentle CTAB and chloroform based DNA precipitation method
Cost effective

Applications
PCR, Real-Time PCR, Southern Blotting, Mapping and RFLP

Caution

GENEzol DNA Reagent Plant contains irritants. During operation, always wear a lab coat, disposable gloves, protective
goggles and (anti-fog) procedure mask.

Additional Requirements

mortar and pestle, 1.5 ml microcentrifuge tubes or 15 ml centrifuge tubes, absolute ethanol for preparing 70% ethanol in
water, chloroform, isopropanol, TE buffer or ddH2O

Components and Storage

Item
GENEzol DNA Reagent Plant

RNase A (50 mg/ml)

Volume

Product

Shipping

Storage

4 ml
100 ml
200 ml
N/A
50 l
100 l

GR004
GR100
GR200
GR004
GR100
GR200

room temperature

dry at room temperature (15-25C)

for up to 1 year

room temperature

4C for extended periods

GENEzol DNA Reagent Plant Functional Test Data

Figure 1. Genomic DNA (approximately 30 kb) was extracted using GENEzol DNA Reagent Plant. 50 mg of fresh
Arabidopsis leaves were initially ground in GENEzol DNA Reagent Plant. A 15 l aliquot of extracted genomic DNA
from a 50 l eluate was analyzed by electrophoresis on a 1% agarose gel.
M = Geneaid 1 Kb DNA Ladder

Test
1
2

Ver. 05.28.15

DNA Concentration
380.7 g/ml
316.9 g/ml

260/280
1.97
1.96

260/230
1.88
1.89

Yield
19.04 g
15.85 g

www.geneaid.com

GENEzol DNA Reagent Plant Page 1 of 4

Fast PCR Grade DNA Protocol Procedure

Please read the entire instruction manual prior to starting the Protocol Procedure.
Use this protocol procedure for purifying PCR grade DNA for routine PCR assays.

1. Plant Tissue Homogenization

1. Cut off 50 mg of fresh plant tissue or 25 mg of dry plant tissue.
2. Freeze the sample with liquid nitrogen (some plant samples can be disrupted without liquid nitrogen).
3. Grind the sample to a fine powder using a mortar and pestle.

2. Lysis
1. Add 800 l of GENEzol Reagent Plant and 0.5 l of RNase A to the sample in the mortar.
2. Continue grinding the sample until it is completely dissolved.
3. Transfer the sample lysate to a 1.5 ml microcentrifuge tube.
4. Incubate the sample lysate at 65C for 15 minutes then centrifuge at 14-16,000 x g for 3 minutes.
5. Transfer the supernatant to a new 1.5 ml microcentrifuge tube.

3. DNA Precipitation
1. Add 600 l of isopropanol to the supernatant in the 1.5 ml microcentrifuge tube.
2. Mix the sample by gently inverting 20 times then let stand for 5 minutes at room temperature.
3. Centrifuge at 14-16,000 x g for 15 minutes to form a tight, well formed DNA pellet.
4. Carefully remove the supernatant then add 1 ml of 70% ethanol to the DNA pellet and wash by gently inverting 20 times.
5. Centrifuge at 14-16,000 x g for 3 minutes.
6. Carefully remove the supernatant then air-dry the DNA pellet for 10-15 minutes at room temperature.
NOTE: DO NOT dry the DNA pellet by vacuum centrifuge and avoid over drying the DNA pellet.
7. Add 50-100 l of TE buffer or ddH2O to the DNA pellet then incubate at 65C for 5 minutes to dissolve the DNA.
NOTE: Occasionaly tapping the bottom of the tube during incubation will promote DNA rehydration.

8. Centrifuge at 14-16,000 x g for 1 minute then transfer the supernatant (containing the purified DNA) to a clean 1.5 ml microcentrifuge tube.
The purified DNA is ready for routine PCR assays.

High Purity and High Yield DNA Protocol Procedure

Please read the entire instruction manual prior to starting the Protocol Procedure.
Use this protocol procedure for purifying high purity and high yield DNA.

1. Plant Tissue Homogenization

1. Cut off 100 mg of fresh plant tissue or 50 mg of dry plant tissue.
2. Freeze the sample with liquid nitrogen (some plant samples can be disrupted without liquid nitrogen).
3. Grind the sample to a fine powder using a mortar and pestle.

2. Lysis
1. Add 1 ml of GENEzol Reagent Plant and 0.5 l of RNase A to the sample in the mortar.
NOTE: If using more than 100 mg of plant tissue, scale GENEzol Reagent Plant proportionately (see table on page 3).
2. Continue grinding the sample until it is completely dissolved then transfer the sample lysate to a 1.5 ml microcentrifuge tube.
NOTE: If using more than 100 mg of plant tissue, transfer the sample lysate to a 15 ml centrifuge tube.
4. Incubate the sample lysate at 65C for 30 minutes then centrifuge at 14-16,000 x g for 5 minutes.
5. Transfer the supernatant to a new 1.5 ml microcentrifuge tube or a new 15 ml centrifuge tube for larger sample sizes.

3. DNA Extraction
Standard Samples:
1. Add 600 l of chloroform to the supernatant.
NOTE: Scale the chloroform proportionately if using larger sample sizes (see table on page 3).
2. Shake the tube vigorously then centrifuge at 14-16,000 x g for 5 minutes.
3. Carefully remove the upper layer and transfer it to a new 1.5 ml microcentrifuge tube or a new 15 ml centrifuge tube for larger sample sizes.
High Polysaccharide Samples:
1. Add a 1/10 volume of GENEzol Reagent Plant and 600 l of chloroform to the supernatant from Step 2
NOTE: Scale GENEzol Reagent Plant and chloroform proportionately if using larger sample sizes (see table on page 3).
2. Shake the tube vigorously then centrifuge at 14-16,000 x g for 5 minutes.
3. Carefully remove the upper layer and transfer it to a new 1.5 ml microcentrifuge tube or a new 15 ml centrifuge tube for larger sample sizes.

GENEzol DNA Reagent Plant Page 2 of 4

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Ver. 05.28.15

4. DNA Precipitation
1. Add 800 l of isopropanol to the 1.5 ml microcentrifuge tube containing the upper layer from step 3.
NOTE: Scale isopropanol proportionately if using larger sample sizes (see table below).
2. Mix the sample by gently inverting 20 times then let stand for 5 minutes at room temperature.
NOTE: DNA precipitation can be increased with extended standing time.
3. Centrifuge at 14-16,000 x g for 20 minutes to form a tight, well formed DNA pellet.
4. Carefully remove the supernatant then add 1 ml of 70% ethanol to the DNA pellet and wash by gently inverting 20 times.
5. Centrifuge at 14-16,000 x g for 3 minutes.
6. Carefully remove the supernatant then air-dry the DNA pellet for 10-15 minutes at room temperature.
NOTE: DO NOT dry the DNA pellet by vacuum centrifuge and avoid over drying the DNA pellet.
7. Add 50-100 l of TE buffer or ddH2O to the DNA pellet then incubate at 65C for 10 minutes to dissolve the DNA.
NOTE: Occasionaly tapping the bottom of the tube during incubation will promote DNA rehydration.

8. Centrifuge at 14-16,000 x g for 1 minute then transfer the supernatant (containing the purified DNA) to a clean 1.5 ml microcentrifuge tube.
The purified DNA is ready for routine PCR assays.

Scaling Large Sample Volumes

Plant tissue
Tube size
GENEzol DNA Reagent Plant
RNase A (50 mg/ml)
Chloroform
Isopropanol
70% ethanol

100 mg
1.5 ml
1 ml
0.5 l
600 l
800 l
1 ml

500 mg
15 ml
5 ml
2.5 l
3 ml
4 ml
5 ml

Troubleshooting
Problem

Cause

Solution

A.

A.

B.

Sample lysis or homogenization was

incomplete
Incorrect DNA precipitation

Low Yield

Slow Rehydration
Eluted DNA does not
perform well in downstream
applications

B.

A.

The DNA pellet is too dry

A.

A.

Residual ethanol contamination

A.

Starting material should be reduced and

completely dissolved in GENEzol DNA
Reagent Plant. Increase incubation time to 1
hour during lysis.
Following isopropanol addition, increase standing
time to improve DNA precipitation. Following
centrifugation, carefully remove the supernatant
without contacting the DNA pellet.
Increase incubation time and tap the bottom of
the tube occasionally to facilitate rehydration
Increase DNA pellet drying time to ensure
residual ethanol is completely evaporated

Related DNA Purification and Extraction Products

Plasmid DNA Purication
Product

Presto Mini Plasmid Kit

Presto Midi Plasmid Kit
Presto Midi Plasmid Kit (Endotoxin Free)
High-Speed Plasmid Mini Kit (10-50 Kb)
High-Speed Plasmid Advance Kit (50-100 ml)
Geneaid Plasmid Mini Kit
Geneaid Plasmid Midi Kit
Geneaid Plasmid Midi Kit (Endotoxin Free)
Presto Plasmid DNA Concentration Kit
Geneaid Plasmid Maxi Kit
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Ver. 05.28.15

Package Size

100/300 preps
25 preps
25 preps
100/300 preps
25 preps
40/100 preps
25 preps
25 preps
250/500/1000 preps
10/25 preps
10/25 preps
4/10 x 96 preps
10 plates
10 plates

www.geneaid.com

Catalogue Number

PDH100/300
PIF025
PIFE25
PDL100/300
PA025
PAE040/100
PI025
PIE25
PC0250/500/1000
PM010/25
PME10/25
96PDV04/10, 96PDC04/10
96PBP01
96PFP01

GENEzol DNA Reagent Plant Page 3 of 4

Related DNA/RNA Purification and Extraction Products

Total RNA Purication
Product

Total RNA Mini Kit (Blood/Cultured Cell)

Total RNA Mini Kit (Tissue)
Total RNA Mini Kit (Plant)
Presto Mini RNA Bacteria Kit
Presto Mini RNA Yeast Kit
miRNA Isolation Kit
GENEzol Reagent
GENEzol TriRNA Bacteria Kit
GENEzol TriRNA Pure Kit
TriRNA Pure Kit
RNA Pure Kit

Genomic DNA Purication

Product

Genomic DNA Mini Kit (Blood/Cultured Cell)

Genomic DNA Midi Kit (Blood/Cultured Cell)
Genomic DNA Maxi Kit (Blood/Cultured Cell)
Genomic DNA Mini Kit (Tissue)
gSYNC DNA Extraction Kit
Genomic DNA Mini Kit (Plant)
Genomic DNA Maxi Kit (Plant)
Geneaid DNA Isolation Kit (Blood)
Geneaid DNA Isolation Kit (Bacteria)
Geneaid DNA Isolation Kit (Tissue)
Geneaid DNA Isolation Kit (Cultured Cell)
GENEzol DNA Reagent Plant
Presto Mini gDNA Yeast Kit
Presto Mini gDNA Bacteria Kit
Geneius Micro DNA Extraction Kit
Presto Buccal Swab gDNA Extraction Kit
Presto 96 Well Blood Genomic DNA Extraction Kit

Magnetic Beads DNA/RNA Purication

Product

Magnetic Beads Genomic DNA Kit (Bacteria)

Magnetic Beads Genomic DNA Kit (Blood)
Magnetic Beads Micro gDNA Kit
Magnetic Beads Genomic DNA Kit (Plant)
Magnetic Beads Viral DNA/RNA Kit
Magnetic Beads PCR Cleanup Kit

PCR

Product

Ultra-Pure Taq DNA Polymerase

Ultra-Pure Taq DNA Polymerase w/ dNTPs
Ultra-Pure Taq DNA Polymerase 1.1
Ultra-Pure Taq DNA Polymerase 1.1 w/ dNTPs
Ultra-Pure Taq DNA Polymerase 1.2
Ultra-Pure Taq DNA Polymerase 1.2 w/ dNTPs
HiFi Taq DNA Polymerase
Ultra-Pure Taq PCR Master Mix
Ultra-Pure Taq PCR Master Mix with Dye
dNTP Solution
dNTP Solution
dNTP Set
dCTP
dATP
dGTP
dTTP

Package Size

Catalogue Number

Package Size

Catalogue Number

Package Size

Catalogue Number

Package Size

Catalogue Number

50/100/300 preps
50/100/300 preps
50/100/300 preps
50/100/300 preps
50/100/300 preps
50/100 preps
100/200 rxns
50/100 rxns
50/100/200 rxns
50/100/200 preps
50/100 preps

100/300 preps
25 preps
10/25 preps
50/100/300 preps
100/300 preps
100 preps
10/25 preps
100/1,000 rxns
150/1,500 rxns
150/1,500 rxns
150/1,500 rxns
100/200 rxns
100/300 preps
100/300 preps
100/300 preps
100/300 preps
4/10 x 96 preps

48/96 rxns
48/96 rxns
48/96 rxns
48/96 rxns
48/96 rxns
48/96 rxns

500/2500 U
500/2500 U
500/2500 U
500/2500 U
500/2500 U
500/2500 U
500 U
200/400 rxns
100 rxns
10 mM each, 200 l
25 mM each, 1 ml
100 mM 1 ml x 4
100 mM, 1 ml
100 mM, 1 ml
100 mM, 1 ml
100 mM, 1 ml

RB050/100/300
RT050/100/300
RP050/100/300
RBB050/100/300
RBY050/100/300
RMI050/100
GZR050/100/200
GZB050/100
GZX050/100/200, GZX051/101/201
TRP050/100/200
PR050/100

GB100/300
GDI25
GDM10/25
GT050/100/300
GS100/300
GP100
GPM10/25
GEB100/01K(+)
GEE150/1.5K(+)
GET150/1.5K(+)
GEC150/1.5K(+)
GR100/200
GBY100/300
GBB100/101/300/301
GMB100/300
GSK100/300
96GBP04/10

ME048/96
MB048/96
MM048/96
MP048/96
MV048/96
MC048/96

UT050/250
UTN050/250
UT051/251
UTN051/251
UT052/252
UTN052/252
HT050
UTM200/400
TQMD100
DN200
DN1100
DN4400
DC1000
DA1000
DG1000
DT1000

For additional product information, please visit www.geneaid.com. Thank you!

GENEzol DNA Reagent Plant Page 4 of 4

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Ver. 05.28.15