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Bioreactor Design

Rahul Thakur submitted a design problem to Er. Himanshu Singh proposing a new bioreactor operation mode that meets five expectations: 1) Minimizes substrate inhibition using a fed batch process, 2) Minimizes product inhibition using a continuous process, 3) Supports collection of gaseous products through continuous addition and removal, 4) Supports simultaneous production of primary and secondary metabolites through changes in conditions, and 5) Uses a single compartment bioreactor that can be operated as continuous or fed batch. The problem addresses key issues in bioreactor design like temperature, pH, nutrients, and product removal to control the biological reaction.

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61 views4 pages

Bioreactor Design

Rahul Thakur submitted a design problem to Er. Himanshu Singh proposing a new bioreactor operation mode that meets five expectations: 1) Minimizes substrate inhibition using a fed batch process, 2) Minimizes product inhibition using a continuous process, 3) Supports collection of gaseous products through continuous addition and removal, 4) Supports simultaneous production of primary and secondary metabolites through changes in conditions, and 5) Uses a single compartment bioreactor that can be operated as continuous or fed batch. The problem addresses key issues in bioreactor design like temperature, pH, nutrients, and product removal to control the biological reaction.

Uploaded by

2526rahul
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© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
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Design Problem -1 BTY-378

Bioprocess Engineering

Submitted To: Er. Himanshu Singh

Submitted By:
Rahul Thakur
Roll no: 21
Section: A7703
B.Tech (H) M.Tech Biotech

Problem
Design a new mode of operation which supports the following expectations which are
listed below-

Expectations:-
1. It should support minimise substrate inhibition.
2. It should support minimise product inhibition.
3. It should support collection of gaseous products.
4. It should support simultaneous production of primary and secondary metabolites.
5. It should not contain any more than 2 compartments

This may include modification of standard design of bioreactors.

Key issues in bioreactor design and operation

The goal of an effective bioreactor is to control, contained positively influence the biological
reaction. To accomplish this, we must take into consideration two areas. One is the suitable
reactor parameters for the desired biological, chemical and physical (macro kinetic) system. The
macro kinetic system includes microbial growth and metabolite production. Microbes can
include bacteria, yeast, fungi, and animal, plant, fish and insect cells, as well as other biological
materials.
Bioreactor design
The other area of major importance in bioreactor design involves the bioreaction parameters,
including:
• controlled temperature
• Optimum pH
• Sufficient substrate (usually a carbon source), such as Sugars, proteins and fats
• Water availability
• Salts for nutrition
• Vitamins
• Oxygen (for aerobic processes)
• Gas evolution and
• Product and byproduct removal.

Minimize substrate inhibition…….

1st we should minimize the dilution rate or slow down the speed of the substrate entry
because if we continuously providing the substrate there will accumulate inside the reactor
that is the substrate is in the excess and it will start inhibiting the enzymes. so to overcome
this problem we should use Fed Batch process.

Fig: Bioreactor design


It should support minimize product inhibition…..

Here we can use continuous process because to avoid the feedback inhibition

That is, if we do not remove the product continuously from the system the formed product will
start inhibiting the enzyme at any level in the reaction. so to encounter this problem the use of
continuous bio reactor is the best option

 It should support collection of gaseous products….


As there is outlet for the ejection of the gases present in the bioreactor formed during the
reaction process. as we know that in the continuous process there is continuously
addition and the removal of the product along with the extra gases, so this
process(continuous process) can fulfil this requirement

 It should support simultaneous production of primary and secondary


metabolites.

As it is clear from the growth curve that when


the cell uses the lag phase to adapt to their mew
environment, new enzymes or structural
components may be synthesized there.
Following the lag period growth starts in the
acceleration phase and continues through the
growth. It is the stage where the primary
metabolites are continuously formed in order to
get the production of secondary metabolites
cells must enter in the stationary phase. This all
happens due to change in conditions like
temperature, pH and some chemical effects in
the system.
1. It should not contain any more than 2 compartments

As we used single compartment bioreactor to explain all the conditions of the design
problem. We can operate this bioreactor for both the processes either for continuous or for
fed batch process that is it is a mixed type of bioreactor.

NOTE: Continuous reactions offer increased opportunities for system investigation and analysis.
Because the variables remain unchanged, a benchmark can be determined for the processresults,
and then the effects of even minor changes to physical or chemical variables can be evaluated.
Also, by changing the growth-limiting nutrient, changes in cell composition and metabolic
activity can be tracked. The constancy of continuous bioreaction also provides a more accurate
picture of kinetic constants, maintenance energy and true growth yields. Secondly, continuous
bioreaction provides a higher degree of control than does batch. Growth rates can be regulated
and maintained for extended periods. By varying the dilution rate, biomass concentration can be
controlled. Secondary metabolite production can be sustained simultaneously along with growth

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