Scribd
Upload
223 views2 pages

Daphnia

This research proposal aims to study the effects of coffee and green tea on the heart rate of Daphnia magna. The objectives are to test whether caffeine affects Daphnia heart rate and to study coffee's long-term effects on normal heart rate. The significance is that the results could provide awareness to society about the health effects of consuming coffee and green tea by comparing their organic compounds. The methods would involve measuring Daphnia heart rates before and after exposure to different concentrations of coffee and green tea over 5 days.

Uploaded by

ridzuwan rahimi
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOC, PDF, TXT or read online on Scribd
223 views2 pages

Daphnia

This research proposal aims to study the effects of coffee and green tea on the heart rate of Daphnia magna. The objectives are to test whether caffeine affects Daphnia heart rate and to study coffee's long-term effects on normal heart rate. The significance is that the results could provide awareness to society about the health effects of consuming coffee and green tea by comparing their organic compounds. The methods would involve measuring Daphnia heart rates before and after exposure to different concentrations of coffee and green tea over 5 days.

Uploaded by

ridzuwan rahimi
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOC, PDF, TXT or read online on Scribd
You are on page 1/ 2

BIO300/NOV 2013

TITLE: EFFECT OF COFFEE AND GREEN TEA ON DAPHNIA SP.

PART B:
i)

RESEARCH PROJECT PROPOSAL

OBJECTIVES:
(Minimum of 2 objectives, maximum of 4 objectives)

To test whether caffeine has an effect on heart rate of Daphnia magna


To study the long term effect of coffee on normal heart rate of Daphnia magna

ii)

SIGNIFICANCE OF PROJECT:
(why this project is important? Benefits to the students, society etc.)

This research will indicate how coffee and green tea effect on living organism
such as Daphnia sp. and human. Then it will give awareness to society about the
effect of consuming coffee and green tea to our health. The result of the
experiment can provide the society the benefits and side effects to compare
between these two ingredients. Moreover green tea and coffee is made up of
different organic compound may improve human health. To sum up in a student
life they will come a time that they will consume coffee to stay up to study. In
modern world coffee and green tea consumption had become trending. We need
to look into these 2 item to learn more about it and to educate to the society.

BIO300/NOV 2013

iii)

RESEARCH METHODS:
(add more pages if necessary)
1) Identify the compound that are consist in the coffee and green tea using
HPLC.
2) Prepare different concentration of coffee and green tea.
2) Select a daphnia from beaker.
3) Put Daphnia on the glass slide.
4) Observe and count normal heart rate.
5) Give coffee treatment for 5 minutes.
6) Observe and measure heart rate using microscope.
7) Repeat the experiment using different coffee concentration.
8) Repeat the experiment for 5 days.

iv)

LIST OF EQUIPMENTS/FACILITIES:
1) Microscope
2) Petri Dish
3) Measuring Cylinder
4) Pipette
5) Stopwatch

v)

LIST OF CHEMICALS/MEDIA:
1) Daphnia Magna
2) Coffee
3) Distilled water
4) Green tea

You might also like