25 LSA Jakathi PDF
25 LSA Jakathi PDF
25 LSA Jakathi PDF
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Research Article
Article History
Received : 05.04.2015
Revised : 12.05.2015
Accepted : 18.05.2015
1. Introduction
C. Janaki / Life Science Archives (LSA), Volume 1, Issue 3, Page 182 - 185, 2015
also in the degradation of various xenobiotic
compounds (Barr and Aust, 1994 Pointing, 2001)
including dyes (Glenn and Gold, 1983). Some
white rot fungi produce all three LME while
others produceonly one or two of them (Hatakka,
1994).
Laccase belongs to the blue multicopper
oxidases and participates in cross-linking of
monomers, degradation of polymers, and ring
cleavage of aromatic compounds. It is widely
distributed in higher plants and fungi. It is present
in
Ascomycetes,
Deuteromycetes
and
Basidiomycetes and abundant in lignin-degrading
white-rot fungi. It is also used in the synthesis of
organic substance, where typical substrates are
amines and phenols, the reaction products are
dimers and oligomers derived from the coupling
of reactive radical intermediates. In the present
study we are using white rot fungi as a source of
laccase enzyme for decolourizing textile industry
dye.
2. Materials and Methods
Textile Dyes
The textile dyes Black B, Crimson, Yellow
F3R, Rhodamin and Merrun V was obtained
from Armats Biotek (P) Ltd, located at Chennai.
Culture media
PDA was used to cultivate white rot fungi
Pleurotus ostreatus
Enzyme production in solid-state fermentation:
Lignocellulosic substrate namely wheat
bran were used for laccase production under Solid
state fermentation condition.
Extraction of Enzyme
A volume of 0.2 M Sodium acetate buffer
(pH - 5.8) was added to 12 days old mass culture
and the mycelium was broken completely. Then, it
was kept in the shaker for overnight. Next day the
culture was filtered and centrifuged at 5000 rpm
for 20 minutes. Supernatant was collected in
conical flask. The enzyme was precipitated using
ammonium sulphate. The precipitated solution
was centrifuged at 5000 rpm for 20 minutes.
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C. Janaki / Life Science Archives (LSA), Volume 1, Issue 3, Page 182 - 185, 2015
culturing. The inoculated plates were incubated
for 10 days and the white rot fungi were grown
without any contamination.
Table 1: Estimation of protein
S.
No
Substrates
1.
Wheat Bran
0.778
1.408
1000
g/ml
24
mm
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C. Janaki / Life Science Archives (LSA), Volume 1, Issue 3, Page 182 - 185, 2015
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