Test Instructions

Colorimetric Methods

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Dilution Factors
2 : 1 Dilution Factor
Mix 5 ml of distilled water with 5 ml of sample water together. Run test according
to instructions. Multiply result x 2.
3 : 1 Dilution Factor
Mix 10 ml of distilled water with 5 ml of sample water together. Run test
according to instructions. Multiply result x 3.
5 : 1 Dilution Factor
Mix 40 ml of distilled water with 10 ml of sample water together. Run test
according to instructions . Multiply result x 5.
1 0 : 1 Dilution Factor
Mix 45 ml of distilled water with 5 ml of sample water together. Run test
according to instructions. Multiply result x 10.
25 : 1 Dilution Factor
Mix 48 ml of distilled water with 2 ml of samp le water together. Run test
according to instructions. Multiply result x 25.

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44720

Instructions for: Acidity

Method: Methyl Orange

Range: 0 25 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.) Scroll down to desired test and press enter. This
will allow the instrument to warm up while preparing the reagent test vial, thus saving time.
2. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
3. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested
to the vial
c. Recap and invert several times
d. Thoroughly wipe the vial clean
e. Allow 5 minutes for reaction
4. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
5. Press enter to continue and follow instructions on screen
6. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS
Select desired test to allow the instrument to warm up
Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results
Rinse 5 ml syringe with distilled water when finished
Be sure to allow 5 minutes for reaction in step 3e
Questions? Please call 330-437-0067 for technical assistance

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44720

Instructions for: Alkalinity

Method: Colorimetric

Range: 0 50 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.) Scroll down to desired test and press enter. This
will allow the instrument to warm up while preparing the reagent test vial, thus saving time.
2. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
3. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of distilled water to the
vial
c. Recap and invert several times
d. Uncap vial and carefully add exactly 5 drops of Alkalinity Reagent # 1
e. Recap and invert several times to mix
f. Uncap and add 2 ml of the water to be tested to the vial
g. Recap and invert several times to mix
h. Thoroughly wipe the vial clean
i. Allow 30 seconds for reaction
4. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
5. Press enter to continue and follow instructions on screen
6. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS
Select desired test to allow the instrument to warm up
Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results
Rinse 5 ml syringe with distilled water when finished
Be sure to use distilled water in step 3b
Carefully add exactly 5 drops of reagent # 1 in step 3d
Be sure to use exactly 2 ml of water to be tested in step 3f
If water contains more than 50 mg/l, then a dilution will be necessary prior to testing
Questions? Please call 330-437-0067 for technical assistance.

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44720

Instructions for: Aluminum

Method: Aluminum (Standard Methods)

Range: 0 2 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.)
Scroll down to desired test and press enter. This will allow the instrument to warm up while
preparing the reagent test vial, thus saving time.
2. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
3. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested
to the vial
c. Recap and invert 20 times
d. Thoroughly wipe the vial clean
e. Allow 20 minutes for reaction
4. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
5. Press enter to continue and follow instructions on screen
6. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS
Select desired test to allow the instrument to warm up
Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results
Rinse 5 ml syringe with distilled water when finished
Must allow 20 minutes for reaction in step 3e
Fluoride may interfere with test
Questions? Please call 330-437-0067 for technical assistance

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44720

Instructions for: Ammonia

Method: Nesslerization (USEPA Approved)

Range: 0 5 mg/L (ppm)

1. Press On I Enter key to power up the instrument. (Please refer to General Operating
Instructions for the Colorimeter for further instructions.)
2. Scroll down to desired test and press enter. This will allow the instrument to warm up while
preparing the reagent test vial, thus saving time.
3. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
4. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested
to the vial
c. Shake dropper bottle marked Ammonia Reagent # 2 and carefully add exactly 9 drops
d. Thoroughly wipe the vial clean
5. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
6. Press enter to continue and follow instructions on screen
7. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS
Select desired test to allow the instrument to warm up
Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results
Rinse 5 ml syringe with distilled water when finished
Be sure to choose Ammonia Nes when selecting test on instrument
Very important to add exactly 9 drops of regent #2
If EPA approved method is required, then a distillation procedure is necessary
Interferences: Calcium, Iron, Magnesium and Sulfide may precipitate with the reagent and
should be removed with Zinc Sulfate and Alkali
For very low concentrations of ammonia, a distillation process may be used
Questions? Please call 330-437-0067 for technical assistance

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44720

Instructions for: Ammonia Salicylate

Method: Salicylate

Range: 0 1 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.)
2. Scroll down to desired test and press enter. This will allow the instrument to warm up while
preparing the reagent test vial, thus saving time.
3. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
4. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested
to the vial
c. Recap and shake 40 times to mix
d. Uncap vial and carefully add exactly 10 drops of Reagent A
e. Recap and shake 40 times to mix
f. Uncap vial and carefully add 2 drops of Reagent B
g. Recap and shake 40 times to mix
h. Thoroughly wipe the vial clean
i. Allow 25 minutes for reaction
5. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
6. Press enter to continue and follow instructions on screen
7. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS
Select desired test to allow the instrument to warm up
Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results
Rinse 5 ml syringe with distilled water when finished
Be sure to add correct amount of drops in steps 4d and 4f
Be sure to shake 40 times to mix
Be sure to allow 25 minutes for reaction
Questions? Please call 330-437-0067 for technical assistance

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44720

Instructions for: Arsenic

Method: Silver Diethyldithiocarbamate (USEPA Approved)

Range: 0 - 0.5 mg/L

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.) Scroll down to desired test and press enter. This
will allow the instrument to warm up while preparing the reagent test vial, thus saving time.
Preliminary Procedure (Caution!! This test contains hazardous materials. Use of fume
hood is recommended. Refer to MSDS for proper handling.)
2. Transfer 40 ml of the water to be tested into the conical flask while stirring using a Hotplate I
Stirrer (It is recommended that a magnetic stir bar be used also)
3. Carefully add exactly 5 mL of Reagent A (Use caution: contains Concentrated
Hydrochloric Acid. Extremely corrosive!)
4. Carefully add exactly 2 mL of Reagent B
5. Carefully add exactly 8 drops of Reagent C
6. Allow solution to stir for 10-15 minutes
7. While the solution is stirring in step 3e, insert glass wool (provided) loosely into scrubber
assembly as shown in figure 1
8. Impregnate the wool with 8 drops of Reagent D (Be sure Reagent D does not drip out of
scrubber tube)

9. Carefully add exactly 5 mL of the indicator solution to the absorber tube (see figure 1)

Arsine generator assembly

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Arsenic Reagent Instructions - Page 2

10. Carefully connect the scrubber tube to the absorber tube using the metal clamp. After solution
has stirred in step 3e, add the entire contents of capsule from the bag labeled Zinc.
11. Immediately connect the scrubber/absorber assembly to the conical flask. Be sure the
connection is firm. Vacuum grease (not included) can be used in this joint, however if the
connection is made properly, this is not necessary. If the connections are made properly, there
should be bubbles coming through absorber assembly. Allow 30 minutes for solution to
complete reaction
12. After 30 minutes, add heat to the conical flask for 3-4 minutes to ensure the reaction is
complete.
13. Preparing the blank vial
a. Select the capped vial marked Arsenic Blank (supplied with each test and will be used
for all 50 tests)
b. Thoroughly wipe the vial clean
14. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Disconnect the absorber tube from the scrubber tube by removing the clamp
c. Fill the empty vial with contents of the absorber tube
d. Recap and thoroughly wipe the vial clean
15. Performing the test
a. Insert the cleaned blank vial in the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
16. Press enter to continue and follow instructions on screen
17. After completion of test, discard the solution in the glassware and vial according to EPA
regulations
IMPORTANT & HELPFUL STEPS
Select desired test to allow the instrument to warm up
Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wipi ng clean
with a dry towel. Foreign residue of any kind or smudges from handling may affect results
Rinse all glassware with distilled water when finished
Be sure all connections are tight
It may not be necessary to add 8 drops of Reagent D to every test that is performed. If upon
successive tests the glass wool is very moist and there are no color changes in the wool,
addition of Reagent D may not be necessary.
Question? Please call 330-437-0067 for technical assistance
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44720

Instructions for: Barium

Method: Turbidimetric

Range: 0 50 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.) Scroll down to desired test and press enter. This
will allow the instrument to warm up while preparing the reagent test vial, thus saving time.
2. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
3. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested
to the vial
c. Recap and invert several times to mix
d. Thoroughly wipe the vial clean
e. Allow 5 minutes for reaction
4. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
5. Press enter to continue and follow instructions on screen
6. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS
Select desired test to allow the instrument to warm up
Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results
Rinse 5 ml syringe with distilled water when finished
Be sure to allow 5 minutes for reaction in step 3e
Questions? Please call 330-437-0067 for technical assistance

6657 Frank Avenue NW

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44720

Instructions for: Boron

Method: Carmine Red (Standard Methods)

Range: 0 1 5 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.) Scroll down to desired test and press enter. This
will allow the instrument to warm up while preparing the reagent test vial, thus saving time.
2. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with distilled water
b. Thoroughly wipe the vial clean
3. Preparing the reagent test vial (Caution!! This test contains Concentrated Sulfuric Acid and
Hydrochloric Acid. Refer to MSDS for proper handling.) Uncap one of the reagent test vials
a. Pipette 5 ml of the Concentrated Sulfuric Acid into the vial
b. Recap vial and set aside
c. Rinse the pipette several times with distilled water before next step
d. Pipette I ml of the water to be tested into a conical flask
e. Carefully add exactly 1 drop of Concentrated Hydrochloric Acid to the flask. (Make sure
the drop hits the water or inconsistent readings may occur..) Carefully add exactly 5 ml
of Concentrated Sulfuric Acid to the flask
f. Swirl solution in flask for at least 1 minute. (Make sure the solution is thoroughly mixed.)
g. Allow the flask to cool to room temperature
h. Shake contents in the vial in step 4a 4c until thoroughly dissolved
i. Pour contents of the vial into the flask
j. Swirl solution in flask for at least 1 minute. (Make sure the solution is thoroughly mixed.)
k. Allow solution in flask to set for 30 to 45 minutes for full color development
l. Uncap clean empty vial and carefully fill with solution from flask
m. Recap and thoroughly wipe the vial clean
4. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
5. Press enter to continue and follow instructions on screen
6. After completion of test, discard the reagent vial according to EPA regulations

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IMPORTANT & HELPFUL STEPS

Page 2

Use Caution when performing procedure. Consult MSDS for proper handling.
Select desired test to allow the instrument to warm up
Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results
Be sure to follow instructions and add exact amounts called for in procedure
When finished, rinse all glassware with 1 + 1 Nitric Acid and follow with several rinses of
distilled water
Questions? Please call 800-543-9283 for technical assistance

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44720

Instructions for: Bromine

Method: DPD (Standard Methods)

Range: 0 - 8 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.) Scroll down to desired test and press enter. This
will allow the instrument to warm up while preparing the reagent test vial, thus saving time.
2. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
3. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested
to the vial
c. Recap and invert several times
d. Thoroughly wipe the vial clean
e. Allow one minute for reaction
4. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
5. Press enter to continue and follow instructions on screen
6. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS
Select desired test to allow the instrument to warm up
Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or
smudges from handling may affect results
Rinse 5 ml syringe with distilled water when finished
Be sure to allow 1 minute for reaction in step 4e
Questions? Please call 330-437-0067 for technical assistance

6657 Frank Avenue NW

Suite 200
North Canton, Ohio
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44720

Instructions for: Cadmium

Method: Dithizone (Standard Methods)

Range: 0 1 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions) Scroll down to desired test and press enter. This will
allow the instrument to warm up while preparing the reagent test vial, thus saving time.
2. Preparing the blank vial (Caution! ! This test contains Chloroform. Use of a fum e hood is
recommended. Refer to MSDS for proper handling.)
a. Select one clean empty vial (supplied with each test kit) and fill with Chloroform and
recap.
b. Thoroughly wipe the vial clean
3. Preparing the reagent test vial
a. Transfer 10 ml of the water to be tested into a 125 ml Separatory funnel
b. Carefully add exactly 0.4 ml of Reagent A to the funnel.
c. Insert the stopper and carefully shake for 10 seconds to mix
d. Carefully add exactly 2 ml of Reagent B to the funnel
e. Insert the stopper and carefully shake for 10 seconds to mix
f. Carefully add exactly 0.4 ml of Reagent C to the funnel
g. Insert the stopper and carefully shake for 10 seconds to mix
h. Carefully add exactly 6 ml of Stock Dithizone solution to the funnel
i. Insert the stopper and carefully shake for 1 minute to mix. Open stopper about every 15
seconds to relieve pressure
j. Drain bottom layer into a 25 ml glassware and keep for additional steps
k. Carefully add exactly 4 ml of Chloroform to the funnel
l. Insert the stopper and carefully shake for 10 seconds to mix. Open stopper to relieve
pressure
m. Drain bottom layer into the 25 ml glassware used in step 4j
n. Drain remaining solution from funnel and discard according to EPA Regulations
o. Rinse funnel thoroughly with distilled water
p. Pour solution from 25 ml glassware in step 4m back into funnel
q. Carefully add exactly 10 ml of Reagent D to the funnel
r. Insert the stopper and carefully shake for 10 seconds to mix. Open stopper to relieve
pressure
s. Drain bottom layer from funnel and discard according to EPA Regulations
t. Carefully add exactly 2 ml of Chloroform to the funnel
u. Insert the stopper and carefully shake for 10 seconds to mix. Open stopper to relieve
pressure
v. Drain bottom layer from funnel and discard according to EPA Regulations
w. Carefully add exactly 0. 1 ml of Reagent C to the funnel
x. Carefully add exactly 6 ml of Working Dithizone solution (1 0 % of Stock Solution) to the
funnel. Working Dithizone Solution must be prepared daily or before each test.
(See Helpful Hints for preparation)
y. Carefully add exactly 2 ml of Reagent E to the funnel

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Cadmium - Reagent Instructions page 2

z. Insert the stopper and carefully shake for 10 seconds to mix. Open stopper to relieve
pressure
aa. Drain the bottom layer into a clean empty vial with this solution and cap. This is the vial
for the test
bb. Allow I minute for reaction
cc. Thoroughly wipe the vial
dd. Discard remaining solution according to EPA Regulations
4. Performing the test
a. Insert the cleaned blank vial into the cuvette opening ofthe Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
5. Press enter to continue and follow instructions on screen
6. After completion of test, discard solution from test preparation and vials according to EPA
regulations
IMPORTANT & HELPFUL STEPS
(Caution!! This test contains Chloroform. Use of a fume hood is recommended. Refer
to MSDS for proper handling.)
Select desired test to allow the instrument to warm up
Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results
When finished, rinse all glassware with I + 1 Nitric Acid and follow with several rinses of
distilled water
Fill Blank vial with Chloroform and cap
Working Dithizone solution is 10 % of Stock Dithizone solution. Mix .6 ml of Stock Solution with
5.4 ml of Chloroform.
Working Dithizone solution must be prepared daily or before each test.
Care must be taken when draining layers

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44720

Instructions for: Calcium Hardness

Method: Eriochrome Blue Black R

Range: 0 300 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.) Scroll down to desired test and press enter. This
will allow the instrument to warm up while preparing the reagent test vial, thus saving time.
2. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
3. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of distilled water to the
vial
c. Recap and invert several times
d. Uncap vial and carefully add exactly 5 drops of Calcium Reagent #2
e. Recap and invert 5 times to mix
f. Uncap the vial and use the 1 ml syringe (supplied with box of reagents) to add 0.2 ml of
the water to be tested to the vial
g. Recap and invert 5 times to mix
h. Thoroughly wipe the vial clean
4. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
5. Press enter to continue and follow instructions on screen
6. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS
Select desired test to allow the instrument to warm up
Be sure the outside of the vial is thoroughly cleaned by dipping vial in wa ter and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results
Rinse 5 ml syringe with distilled water when finished
Be sure to use distilled water in step 3b
Carefully add exactly 5 drops of reagent # 2 in step 3d
Carefully add exactly 0.2 ml of the water to be tested In step 3f

Please call 330 437-0067 for technical assistance.

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44720

Instructions for: Carbon Dioxide

Method: Colorimetric

Range: 0 500 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.) Scroll down to desired test and press enter. This
will allow the instrument to warm up while preparing the reagent test vial, thus saving time.
2. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
3. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested
to the vial
c. Recap and invert until all chemicals have dissolved. Do not shake vial
d. Uncap vial and carefully add exactly 1 drop of Carbon Dioxide reagent A
e. Recap and invert 5 times to mix
f. Thoroughly wipe the vial clean
4. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
5. Press enter to continue and follow instructions on screen
6. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS

Select desired test to allow the instrument to warm up

Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affec t
results

Rinse 5 ml syringe with distilled water when finished

Do not shake vial in step 3c

Care must be taken to add exactly one drop of reagent A in step 3d

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44720

Instructions for: Chloramine T

Method: DPD

Range: 0 20 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.)
2. Scroll down to desired test and press enter. This will allow the instrument to warm up while
preparing the reagent test vial, thus saving time.
3. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
4. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested
to the vial
c. Recap and invert several times
d. Thoroughly wipe the vial clean
e. Allow 5 minutes for reaction
5. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
6. Press enter to continue and follow instructions on screen
7. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS

Select desired test to allow the instrument to warm up

Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results

Rinse 5 ml syringe with distilled water when finished

Be sure to allow 5 minutes for reaction in step 4e

Questions? Please call 330-437-0067 for technical assistance

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44720

Instructions for: Chloride

Method: Colorimetric

Range: 0 300 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.) Scroll down to desired test and press enter. This
will allow the instrument to warm up while preparing the reagent test vial, thus saving time.
2. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
3. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested
to the vial
c. Recap and shake for 5 seconds
d. Uncap vial and carefully add exactly 10 drops of Chloride Reagent #2
e. Recap and invert several times to mix
f. Thoroughly wipe the vial clean
g. Allow 10 minutes for reaction
h. Un-dissolved reagent should settle on bottom and not be disturbed when handling
4. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, gently insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
5. Press enter to continue and follow instructions on screen
6. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS

Select desired test to allow the instrument to warm up

Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results

Rinse 5 ml syringe with distilled water when finished

Carefully add exactly 10 drops in step 3d

Be sure to allow 10 minutes for reaction in step 3g

Be sure to gently place the test vial into Colorimeter in step 5c so that the un-dissolved reagent
is not disturbed

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44720

Instructions for: Chlorine Free

Method: DPD (USEPA Approved)

Range: 0- 4 mg/L (ppm)

1.

Press On/Enter key to power up the instrument. (Please refer to General Operating
Instructions for the Colorimeter for further instructions.)

2.

Scroll down to desired test and press enter. This will allow the instrument to warm up while
preparing the reagent test vial, thus saving time.

3.

Preparing the blank vial

a. Select the uncapped empty vial (supplied with each test kit) and fill with
the water to be tested
b. Thoroughly wipe the vial clean

4.

Preparing the reagent test vial

a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the
water to be tested to the vial
c. Recap and invert several times
d. Thoroughly wipe the vial clean
e. Allow one minute for reaction

5.

Performing the test

a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
f. Press enter to continue and follow instructions on screen

6.

After completion of test, discard the reagent vial according to EPA regulations

IMPORTANT & HELPFUL STEPS

Select desired test to allow the instrument to warm up

Be sure the outside of the vial is thoroughly cleaned by dipping vial in wa ter and wiping clean
with a dry paper towel.
Foreign residue of any kind or smudges from handling may affect results
Rinse 5 ml syringe with distilled water when finished
Shelf life is no longer than 6 months

Questions? Please call 330-437-0067 for technical assistance

6657 Frank Avenue NW

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44720

Instructions for: Chlorine Total

Method: DPD (USEPA Approived)

Range: 0 4 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.)
2. Scroll down to desired test and press enter. This will allow the instrument to warm up while
preparing the reagent test vial, thus saving time.
3. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
4. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested
to the vial
c. Recap and invert several times
d. Thoroughly wipe the vial clean
e. Allow one minute for reaction
5. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
f. Press enter to continue and follow instructions on screen
6. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & ITELPFUL STEPS
Select desired test to allow the instrument to warm up
Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results
Rinse 5 ml syringe with distilled water when finished
Shelf life is no longer than 6 months
Questions? Please call 330-437-0067 for technical assistance

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44720

Instructions for: Chromate

Method: Colorimetric

Range: 0 100 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.)
2. Scroll down to desired test and press enter. This will allow the instrument to warm up while
preparing the reagent test vial, thus saving time.
3. Preparing the blank vial
a. Select the vial marked Blank (supplied with each test kit and to be used for all 50
tests)
b. Thoroughly wipe the vial clean
4. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested
to the vial
c. Recap
d. Thoroughly wipe the vial clean
5. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
6. Press enter to continue and follow instructions on screen
7. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS

Select desired test to allow the instrument to warm up

Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results

Rinse 5 ml syringe with distilled water when finished

Questions? Please call 330-437-0067 for technical assistance

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44720

Instructions for: Color

Method: APHA Platinum Cobalt (Standard Methods)

Range: 0 500 APHA

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.)
2. Scroll down to desired test and press enter. This will allow the instrument to warm up while
preparing the reagent test vial, thus saving time.
3. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with distilled w ater
Thoroughly wipe the vial clean
4. Preparing the reagent test vial
a. Uncap one of the reagent test vials (all vials are empty) and fill with the water to be
tested
b. Recap and thoroughly wipe the vial clean
5. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
6. Press enter to continue and follow instructions on screen
7. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS
Select desired test to allow the instrument to warm up
Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results
Questions? Please call 330-437-0067 for technical assistance

6657 Frank Avenue NW

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44720

Instructions for: Copper

Method: Bathocuporine (Standard Methods)

Range: 0 3 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.)
2. Scroll down to desired test and press enter. This will allow the instrument to warm up while
preparing the reagent test vial, thus saving time.
3. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
4. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested
to the vial
c. Recap and invert several times
d. Thoroughly wipe the vial clean
5. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
f. Press enter to continue and follow instructions on screen
6. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS
Select desired test to allow the instrument to warm up
Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results
Rinse 5 ml syringe with distilled water when finished
pH of the water to be tested should be between 3 and 6

Questions? Please call 330-437-0067 for technical assistance

6657 Frank Avenue NW

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44720

Instructions for: Cyanide Free

Method: Pyridine Pyrazalone (Standard Methods)

Range: 0 0.3 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.)
2. Scroll down to desired test and press enter. This will allow the instrument to warm up while
preparing the reagent test vial, thus saving time.
3. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested
to the vial.
c. Uncap Test Vial (test vial #1) and add 1 level spoon of Reagent #1, cap, and invert 20
times.
d. Fill an empty vial (test vial #2) with 5mL of sample to test and add 1 level scoop of
Reagent #2 to this vial. Invert 60 times and allow 2 minutes for reaction.
e. Combine the contents of Test Vial #1 and Test Vial #2 into a 10 ml graduated cylinder.
Invert 20 times and allow to react for 5 minutes. Re-pour a portion of test solution back
into Test Vial #1 (3/4 full).
f. Thoroughly wipe the vial clean
4. Preparing the blank vial
a. Add 5 mL of sample water to be tested into test vial #2. This will become the blank.
b. Thoroughly wipe the vial clean
5. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
6. Press enter to continue and follow instructions on screen
7. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS

Select desired test to allow the instrument to warm up

Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results

Rinse 5 ml syringe and capped vial with distilled water when finished

Questions? Please call 330-437-0067 for technical assistance

Instructions for: Cyanuric Acid

Method: Turbidimetric

Range: 0 200 mg/L (ppm)

6657 Frank Avenue NW

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44720

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.)
2. Scroll down to desired test and press enter. This will allow the instrument to warm up while
preparing the reagent test vial, thus saving time.
3. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
4. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested
to the vial
c. Recap and invert several times to mix
d. Thoroughly wipe the vial clean
5. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
6. Press enter to continue and follow instructions on screen
7. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS

Select desired test to allow the instrument to warm up

Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results

Rinse 5 ml syringe with distilled water when finished

Questions? Please call 330-437-0067 for technical assistance

6657 Frank Avenue NW

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North Canton, Ohio
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44720

Instructions for: Detergents

Method: Methylene Blue
Range: 0 5 mg/L (ppm)
1. Press On/Enter key to power up the instrument (Please refer to General Operating Instructions
for the Colorimeter for further Instructions.)
2. Scroll down to desired test and press enter. This will allow the instrument to warm up while
preparing the reagent test vial, thus saving time.
3. Preparing the blank vial
a. Select one clean empty vial (supplied with each test kit) and fill with chloroform.
b. Recap and thoroughly wipe the vial clean
4. Preparing the reagent test vial (Caution!! This test contains Chloroform and Sulfuric
Acid Solution. Use of a fume hood is recommended. refer to MSDS for proper
handling.)
a. Transfer 10 ml of the water to be tested into the Separatory funnel
b. Carefully add exactly 1 ml of indicator solution to the funnel
c. Swirl funnel to mix solution
d. Carefully add exactly 7 drops of Reagent # I to the funnel
e. Insert the stopper and carefully shake for 30 seconds
f. Place funnel back in upright position and open stopper to relieve pressure
g. Carefully add 2 drops of Reagent # 2 to the funnel
h. Reinsert the stopper and shake for 20 seconds
i. Place funnel back in upright position and open stopper to relieve pressure
j. Carefully add exactly 2 drops of Reagent #3
k. Reinsert the stopper and shake for 20 seconds
l. Place funnel back in upright position and open stopper to relieve pressure
m. Carefully add exactly 1 0 ml of Chloroform to the funnel
n. Reinsert the stopper and shake for 20 seconds
o. Place funnel back in upright position and open stopper to relieve pressure
p. Carefully add exactly 7 drops of Reagent #4
q. Reinsert the stopper and shake for 20 seconds
r. Place funnel back in upright position and open stopper to relieve pressure
s. Allow the layers to separate for 30 seconds
t. Drain solution from the bottom layer of the funnel into a clean empty vial
u. Thoroughly wipe the vial clean
5. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
6. Press enter to continue and follow instructions on screen
7. After completion of test, discard solution in the funnel and vials according to EPA regulations

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44720

Detergents Reagent Instructions

Page 2

IMPORTANT & HELPFUL STEPS

Use caution when performing procedure. Consult MSDS for proper care.

Select desired test to allow the instrument to warm up

Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results

When finished, rinse all glassware with 1 + 1 Nitric Acid and follow with several rinses of
distilled water

Be sure to add correct amounts called for in procedure

Questions? Please call 330-437-0067 for technical assistance

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44720

Instructions for: Dissolved Oxygen

Method: Azide Modification (Standard Methods)

Range: 0 10 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.) Scroll down to desired test and press enter. This
will allow the instrument to warm up while preparing the reagent test vial, thus saving time.
2. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
3. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 8 ml (4 ml twice) of the water
to be tested to the vial
c. Carefully add exactly 2 drops of reagent A and 2 drops of reagent B
d. Recap and invert for 1 minute to mix
e. Allow 2 minutes for reaction
f. Uncap vial and carefully add exactly 20 drops of Reagent C
g. Recap and invert for I minute to mix
h. Allow 5 minutes for reaction (If reagent is not dissolved, shake I minute and allow
another 5 minutes for reaction)
i. Thoroughly wipe the vial clean
4. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
5. Press enter to continue and follow instructions on screen
6. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS

Select desired test to allow the instrument to warm up

Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results

Rinse 5 ml syringe with distilled water when finished

Be sure to add exactly 8 ml of water to be tested to the vial in step 3b

Take care to add correct amount of drops in steps 3c and 3f

Questions? Please call 330-437-0067 for technical assistance
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44720

Instructions for: Fluoride High

Method: Spadns (USEPA Approved)

Range: 0 6 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (P1ease refer to General Operating Instructions
for the Colorimeter for further instructions.) Scroll down to desired test and press enter. This
will allow the instrument to warm up while preparing the reagent test vial, thus saving time.
2. Preparing the blank vial
a. Select one of the reagent vials and uncap
b. Use the 5 ml syringe (supplied with each test kit) and add 2 ml of the 3 ppm Standard
c. Recap and invert several times
d. Place a mark on the cap of this vial (This blank will be used for the entire box)
e. Thoroughly wipe the vial clean
f. Rinse the 5 ml syringe with distilled water before next step
3. Preparing the reagent test vial (If Chlorine is present, follow Step 4)
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 2 ml of the water to be tested
to the vial
c. Recap and invert several times
d. Thoroughly wipe the vial clean
e. Follow Step 5
4. Preparing the reagent test vial (only if Chlorine is present)
a. In a separate clean container, fill with 25 ml of the water to be tested
b. Carefully add exactly 4 drops from dropper bottle labeled Sodium Arsenite to the 25 ml
container and mix
c. Uncap one of the reagent test vials
d. Use the 5 ml syringe (supplied with Colorimeter) and add 2 ml of the 25 ml solution
mixed with Sodium Arsenite to the vial
e. Recap and invert several times
f. Thoroughly wipe the vial clean
g. Discard the remaining 23 ml Sodium Arsenite solution
5. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
6. Press enter to continue and follow instructions on screen
7. After completion of test, discard the reagent vial according to EPA regulations
6657 Frank Avenue NW
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44720

Instructions for: Fluoride High

Page 2

IMPORTANT & HELPFUL STEPS

Select desired test to allow the instrument to warm up

Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results

Rinse 5 ml syringe with distilled water after each use

The blank should only be used with the box of reagents it came from. Discard blank after each
box of reagents and prepare a new blank with each new box

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44720

Instructions for: Fluoride Low

Method: Spadns (USEPA Approved)

Range: 0 2 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.) Scroll down to desired test and press enter. This
will allow the instrument to warm up while preparing the reagent test vial, thus saving time.
2. Preparing the blank vial
a. Select one of the reagent vials and uncap
b. Use the 5 ml syringe (supplied with each test kit) and add 5 ml of the 1 ppm Standard
c. Recap and invert several times
d. Place a mark on the cap of this vial (This blank will be used for the entire box)
e. Thoroughly wipe the vial clean
f. Rinse the 5 ml syringe with distilled water before next step
3. Preparing the reagent test vial (If Chlorine is present, go to Step 4)
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested
to the vial
c. Recap and invert several times
d. Thoroughly wipe the vial clean
e. Follow Step 5
4. Preparing the reagent test vial (only if Chlorine is present)
a. In a separate clean container, fill with 25 ml of the water to be tested
b. Carefully add exactly 4 drops from dropper bottle labeled Sodium Arsenite to the 25 ml
container and mix
c. Uncap one of the reagent test vials
d. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the 25 ml solution
mixed with Sodium Arsenite to the vial
e. Recap and invert several times
f. Thoroughly wipe the vial clean
g. Discard the remaining 20 ml Sodium Arsenite solution
5. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
6. Press enter to continue and follow instructions on screen
7. After completion of test, discard the reagent vial according to EPA regulations

6657 Frank Avenue NW

Suite 200
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44720

Instructions for: Fluoride Low

Page 2

IMPORTANT & HELPFUL STEPS

Select desired test to allow the instrument to warm up
Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results
Rinse 5 ml syringe with distilled water after each use
The blank should only be used with the box of reagents it came from. Discard blank after each
box of reagents and prepare a new blank with each new box
Questions? Please call 330-437-0067 for technical assistance

6657 Frank Avenue NW

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www.hydrodynesystems.com

44720

Instructions for: Fluoride Low - P

Method: Spadns

Range: 0 2 mg/L (ppm)

1.

Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.)

2.

Scroll down to Fluoride Low test and press enter. This will allow the instrument to warm up
while preparing the reagent test vial, thus saving time.

3.

Preparing the blank vial

a. Select one of the reagent vials and uncap
b. Use the 5 ml syringe (supplied with each test kit) and add 2 ml of the 1 ppm Standard
c. Recap and invert several times
d. Place a mark on the cap of this vial (This blank will be used for the entire box)
e. Thoroughly wipe the vial clean
f. Rinse the 5 ml syringe with distilled water before next step

4.

Preparing the reagent test vial (If Chlorine is present, follow Step 5)
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 2 ml of the water to be tested to
the vial
3. Recap and invert several times
4. Thoroughly wipe the vial clean
5. Follow Step 6

5. Preparing the reagent test vial (Chlorine is present)

a. In a separate clean container, fill with 25 ml of the water to be tested
b. Carefully add exactly 4 drops from dropper bottle labeled Sodium Arsenite to the 25 ml
container and mix
c. Uncap one of the reagent test vials
d. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the 25 ml solution mixed with
Sodium Arsenite to the vial
e. Recap and invert several times
f. Thoroughly wipe the vial clean
g. Discard the remaining 20 ml Sodium Arsenite solution
6. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
f. Press enter to continue and follow instructions on screen
g. After completion of test, discard the reagent vial according to EPA regulations
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44720

Instructions for: Fluoride Low P

Page 2

IMPORTANT & HELPFUL STEPS

Select desired test to allow the instrument to warm up

Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results
Rinse 5 ml syringe with distilled water after each use
The blank should only be used with the box of reagents it came from. Discard blank after each
box of reagents and prepare a new blank with each new box

Questions? Please call 330-437-0067 for technical assistance

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44720

Instructions for: Formaldehyde

Method: Chromotropic Acid Range: 0 100 mg/L (ppm)
1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.) Scroll down to desired test and press enter. This
will allow the instrument to warm up while preparing the reagent test vial, thus saving time.
2. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with distilled water
b. Thoroughly wipe the vial clean
3. Preparing the reagent test vial (Caution!! This test contains Concentrated Sulfuric Acid
and is extremely corrosives Refer to MSDS for proper handling)
a. Carefully add 4 ml of the Concentrated Sulfuric Acid to a 125 ml Erlenmeyer flask
b. Add exactly 1 drop of Regent # 1
c. Swirl flask carefully to mix
d. Immediately add exactly 4 ml of the water to be tested to the flask
e. Swirl flask carefully to mix
f. Allow 10 minutes for reaction
g. Add 5 ml of distilled water to the flask
h. Swirl flask carefully to mix
i. Carefully pipette 5 ml of this solution into a clean empty vial (supplied with test)
j. Thoroughly wipe the vial clean
4. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
5. Press enter to continue and follow instructions on screen
6. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS

Select desired test to allow the instrument to warm up

Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results

Rinse all glassware thoroughly with distilled water

Use caution when performing procedure. Consult MSDS for proper care.

Be sure to add correct amounts listed in each step

Be sure to allow 10 minutes for reaction in step 3f

Questions? Please call 330-437-0067 for technical assistance
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44720

Instructions for: Hex Chrome

Method: 1,5 Diphenylcarbohydrazide (USEPA Approved)

Range: 0 0.7 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.) Scroll down to desired test and press enter. This
will allow the instrument to warm up while preparing the reagent test vial, thus saving time.
2. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
3. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested
to the vial
c. Recap and invert several times
d. Thoroughly wipe the vial clean
e. Allow ten minutes for reaction
4. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
5. Press enter to continue and follow instructions on screen
6. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS

Select desired test to allow the instrument to warm up

Be sure the outside of vial is thoroughly cleaned by dipping vial in water and wiping clean with
a dry paper towel. Foreign residue of any kind or smudges from handling may affect results

Rinse 5 ml syringe with distilled water when finished

Be sure to allow the required amount of time for reaction

If using EZ-Check auto-shut off may occur after 12 minutes

Questions? Please call 330-437-0067 for technical assistance

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44720

Instructions for: Hydrazine

Method: P Dimethylaminabenzaldehyde

Range: 0 0.5 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.) Scroll down to desired test and press enter. This
will allow the instrument to warm up while preparing the reagent test vial, thus saving time.
2. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
3. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested
to the vial
c. Recap and invert 1 0 times to mix
d. Thoroughly wipe the vial clean
e. Allow 10 minutes for reaction
4. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
5. Press enter to continue and follow instructions on screen
6. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS

Select desired test to allow the instrument to warm up

Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may, affect
results

Rinse 5 ml syringe with distilled water when finished

Be sure to analyze the test vial immediately to avoid auto-oxidation. To prevent, place 5 ml of
HCl (1 + 9) in a 50 ml volumetric flask and fill to 50 ml with water to be tested

Use HC1 (1 + 9) for dilution instead of distilled water, if a dilution is required.

Questions? Please call 330-437-0067 for technical assistance

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44720

Instructions for: Iodine

Method: DPD (Standard Methods)

Range: 0 10 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.) Scroll down to desired test and press enter. This
will allow the instrument to warm up while preparing the reagent test vial, thus saving time.
2. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
3. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested
to the vial
c. Recap and invert several times to mix
d. Thoroughly wipe the vial clean
e. Allow one minute for reaction
4. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
5. Press enter to continue and follow instructions on screen
6. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS

Select desired test to allow the instrument to warm up

Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results

Rinse 5 ml syringe with distilled water when finished

Questions? Please call 330-437-0067 for technical assistance

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44720

Instructions for: Iron

Method: Phenanthroline (Standard Methods)

Range: 0 4 mg/L (ppm)

7. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.)
8. Scroll down to desired test and press enter. This will allow the instrum ent to warm up while
preparing the reagent test vial, thus saving time.
9. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
10. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested
to the vial
c. Recap and invert several times
d. Thoroughly wipe the vial clean
e. Allow one minute for reaction
11. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
f. Press enter to continue and follow instructions on screen
12. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS
Select desired test to allow the instrument to warm up
Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or
smudges from handling may affect results
Rinse 5 ml syringe with distilled water when finished
Questions? Please call 330-437-0067 for technical assistance

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44720

Instructions for: Lead

Method: Dithizone (Standard Methods)

Range: 0 2 mg/L (ppm)

1. Press On I Enter key to power up the instrument. (Please refer to General Operating
Instructions for the Colorimeter for further instructions.) Scroll down to desired test and
press enter. This will allow the instrument to warm up while preparing the reagent test vial,
thus saving time.
2. Preparing the blank vial
a. Select one clean empty vial (supplied with each test kit) and fill with chloroform.
b. Thoroughly wipe the vial clean
3. Preparing the reagent test vial (Caution!! This test contains Chloroform and
Potassium Cyanide solution. Use of a fume hood is recommended. Refer to MSDS
for proper handling.)
a. Transfer 10 ml of Chloroform and 10 ml of water to be tested into the Separatory
funnel
b. Carefully add exactly 0.2 ml of the dithizone solution to the funnel
c. Insert the stopper and carefully shake for 10 seconds to mix
d. Place funnel back in upright position and open stopper to relieve pressure
e. Carefully add exactly 2 drops of Reagent # 1 to the funnel
f. Insert the stopper and carefully shake for 30 seconds
g. Place funnel back in upright position and open stopper to relieve pressure
h. Reinsert the stopper and allow 60 seconds for reaction
i. The presence of Lead will cause the bottom half of the solution to be red or
pink.
1. If the solution in the bottom of the funnel is green remove the
stopper and add 1 or 2 drops of reagent Lead reagent# 2 to the
separatory funnel, insert stopper and shake for 30 seconds.

i.

ii. Allow layers to separate for 30 seconds and drain from the bottom layer of
the funnel into the empty vial and fill 3/4 full.
Thoroughly wipe the vial clean

4. Performing the test

a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
5. Press enter to continue and follow instructions on screen
6. After completion of test, discard solution in the funnel and vials according to EPA regulations

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44720

Instructions for: Lead

Page 2

IMPORTANT & HELPFUL STEPS

Select desired test to allow the instrument to warm up

Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling m ay affect
results

When finished, rinse all glassware with 1 + 1 Nitric Acid and follow with several rinses of
distilled water

Interferences: Most metals which form dithizonates are eliminated by double extraction
controlled pH 8.5 9.0. Organic material must be removed by acid digestion.
Lead - Reagent Instructions Page 2

Use caution when performing procedure. Consult MSDS for proper care.

Questions? Please call 330-437-0067 for technical assistance

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44720

Instructions for: Magnesium

Method: Titan Yellow

Range: 0 10 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.) Scroll down to desired test and press enter. This
will allow the instrument to warm up while preparing the reagent test vial, thus saving time.
2. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
3. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 6 ml (3 ml twice) of the water
to be tested to the vial
c. Recap and invert 5 times
d. Uncap vial and carefully add exactly 20 drops of Magnesium Reagent A
e. Recap and invert 5 times to mix
f. Uncap vial and carefully add 5 drops of Magnesium Reagent B
g. Recap and invert 20 times
h. Thoroughly wipe the vial clean
i. Allow one minute for reaction
4. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
5. Press enter to continue and follow instructions on screen
6. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS

Select desired test to allow the instrument to warm up

Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results

Rinse 5 ml syringe with distilled water when finished

Add 6 ml (3m1 twice) of water to be tested in step 3b

Care must be taken when adding drops in steps 3d and 3f

Questions? Please call 330-437-0067 for technical assistance
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44720

Instructions for: Manganese High

Method: Cold Periodate Oxidation (USEPA Approved)

Range: 0 20 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.) Scroll down to desired test and press enter. This
will allow the instrument to warm up while preparing the reagent test vial, thus saving time.
2. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
3. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested
to the vial
c. Recap and invert 60 times
d. Uncap vial and carefully add 1 level spoon of Manganese High Reagent # 1
e. Recap and invert 20 times to mix
f. Thoroughly wipe the vial clean
g. Allow one minute for reaction
4. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
5. Press enter to continue and follow instructions on screen
6. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS

Select desired test to allow the instrument to warm up

Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results

Rinse 5 ml syringe with distilled water when finished

Questions? Please call 330-437-0067 for technical assistance

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44720

Instructions for: Manganese Low

Method: Formaldoxime

Range: 0 3 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.)
2. Scroll down to desired test and press enter. This will allow the instrument to warm up while
preparing the reagent test vial, thus saving time.
3. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
4. Preparing the reagent test vial
a. Uncap one of the reagent test vials (All vials are empty)
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested
to the vial
c. Carefully add exactly 5 drops of reagent # I
d. Immediately add exactly 5 drops of reagent # 2
e. Recap and invert several times to mix
f. Allow (2) two minutes for reaction
g. Uncap vial and carefully add exactly 5 drops of reagent #3
h. Immediately add exactly 5 drops of reagent #4
i. Recap and invert several times to mix
j. Allow (10) ten minutes for reaction
k. Thoroughly wipe the vial clean
l. Allow one minute for reaction
5. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
f. Press enter to continue and follow instructions on screen
6. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS
Select desired test to allow the instrument to warm up
Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results
Rinse 5 ml syringe with distilled water when finished
Questions? Please call 330-437-0067 for technical assistance
6657 Frank Avenue NW
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North Canton, Ohio
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44720

Instructions for: Mercury

Method: Dithizone (Standard Methods)

Range: 0 2 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.)Scroll down to desired test and press enter. This will
allow the instrument to warm up while preparing the reagent test vial, thus saving time.
2. Preparing the blank vial
a. Select one clean empty vial (supplied with each test kit) and fill with chloroform.
b. Recap and thoroughly wipe the vial clean
3. Preparing the reagent test vial (Caution!! This test contains Chloroform. Use of a fume
hood is recommended. Refer to MSDS for proper handling.)
a. Transfer 10 ml of the water to be tested into the Separatory funnel
b. Carefully add exactly 6 drops of Reagent A to the funnel
c. Insert the stopper and carefully shake for 10 seconds to mix
d. Place funnel back in upright position and open stopper to relieve pressure
e. Carefully add exactly 10 ml of Chloroform to the funnel
f. Carefully add exactly 0.2 ml of Dithizone Reagent to the funnel
g. Insert the stopper and carefully shake for 20 seconds
h. Place funnel back in upright position and open stopper to relieve pressure
i. Carefully add exactly 4 drops of Reagent B to the funnel
j. Reinsert the stopper and shake for 20 seconds
k. Place the funnel back in upright position and open stopper to relieve pressure
l. Allow approximately 30 seconds for layers to separate in the funnel
i. The bottom layer should be a shade of orange.
m. Drain solution from bottom layer of the funnel into a clean empty vial
n. Recap and thoroughly wipe the vial clean
4. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
5. Press enter to continue and follow instructions on screen
6. After completion of test, discard solution in the funnel and vials according to EPA regulations
IMPORTANT & HELPFUL STEPS

Use caution when performing procedure. Consult MSDS for proper handling.

Select desired test to allow the instrument to warm up

Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results

When finished, rinse all glassware with 1 + 1 Nitric Acid and follow with several rinses of
distilled water

Be sure to add exact amounts called for in procedure

Questions? Please call 330-437-0067 for technical assistance

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44720

Instructions for: Molybdenum

Method: Mercaptoacetic Acid

Range: 0-35 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.)
2. Scroll down to desired test and press enter. This will allow the instrument to warm up while
preparing the reagent test vial, thus saving time.
3. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
4. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested
to the vial
c. Recap and invert several times until powder is dissolved
d. Uncap vial and carefully add exactly one drop of Molybdenum reagent # 1
e. Recap and invert 10 times to mix
f. Thoroughly wipe the vial clean
g. Allow one minute for reaction
5. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
f. Press enter to continue and follow instructions on screen
g. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS
Select desired test to allow the instrument to warm up
Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results
Rinse 5 ml syringe with distilled water when finished
Powder must be dissolved in step 4c
Care must be taken to add only one drop in step 4d
Allow one minute for reaction in step 4g

If water contains more than 35 mg/i of Molybdenum, than a dilution must be performed before
Step 4
Questions? Please call 330-437-0067 for technical assistance

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44720

Instructions for: Nickel

Method: Dimethyglyoxime
Range: 0 10 mg/L (ppm)
1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.)Scroll down to desired test and press enter. This will
allow the instrument to warm up while preparing the reagent test vial, thus saving time.
2. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
3. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested
to the vial
c. Carefully add exactly 3 drops of Nickel reagent #2
d. Recap and invert 5 times
e. Allow 3 5 minutes for reaction
f. Uncap vial and carefully add exactly 4 drops of Nickel reagent # 3
g. Recap and invert 5 times to mix
h. Allow between 20 25 minutes for reaction
i. Thoroughly wipe the vial clean
4. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
5. Press enter to continue and follow instructions on screen
6. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS

Select desired test to allow the instrument to warm up

Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results

Rinse 5 ml syringe with distilled water when finished

Take extra care when adding stated amount of drops from dropper bottles

Allow required reaction times

Questions? Please call 330-437-0067 for technical assistance

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44720

Instructions for: Nitrate High

Method: Cadmium Reduction (Standard Methods)

Range: 0 100 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.) Scroll down to desired test and press enter. This
will allow the instrument to warm up while preparing the reagent test vial, thus saving tim e.
2. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
3. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of distilled water to the
vial
c. Carefully add exactly 0.2 ml of the water to be tested to the vial
d. Recap and invert several times
e. Uncap vial and carefully add approximately 12 grains from the Cadmium capsule
(amount of grains should approximately be the diameter of an eraser floating in the vial)
f. Recap and shake for 5 7 minutes to mix
g. Thoroughly wipe the vial clean
4. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
5. Press enter to continue and follow instructions on screen
6. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS

Select desired test to allow the instrument to warm up

Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results

Rinse 5 ml syringe with distilled water when finished

Be sure to add 5 ml of distilled water in step 3b

When grains are added in step 3d, color should be pink. If too many grains are added, color will
turn grayish purple
Questions? Please call 330-437-0067 for technical assistance

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44720

Instructions for: Nitrate High

Method: Cadmium Reduction (Standard Methods)

Range: 0 100 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.) Scroll down to desired test and press enter. This
will allow the instrument to warm up while preparing the reagent test vial, thus saving time.
2. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
3. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of distilled water to the
vial
c. Carefully add exactly 0.2 ml of the water to be tested to the vial
d. Recap and invert several times
e. Uncap vial and carefully add approximately 12 grains from the Cadmium capsule
(amount of grains should approximately be the diameter of an eraser floating in the vial)
f. Recap and shake for 5 7 minutes to mix
g. Thoroughly wipe the vial clean
4. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
5. Press enter to continue and follow instructions on screen
6. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS
Select desired test to allow the instrument to warm up
Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results
Rinse 5 ml syringe with distilled water when finished
Be sure to add 5 ml of distilled water in step 3b
When grains are added in step 3d, color should be pink. If too many grains are added, color will
turn grayish purple
Questions? Please call 330-437-0067 for technical assistance
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44720

Instructions for: Nitrate Low

Method: Cadmium Reduction (Standard Methods)

Range: 0 4 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.) Scroll down to desired test and press enter. This
will allow the instrument to warm up while preparing the reagent test vial, thus saving time.
2. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
3. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested
to the vial
c. Recap and invert several times
d. Uncap vial and carefully add a few crystals of Reagent #1 (amount of grains should
approximately be the diameter of an eraser floating in the vial)
e. Recap and shake for 5 7 minutes to mix
f. Thoroughly wipe the vial clean
4. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
5. Press enter to continue and follow instructions on screen
6. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS

Select desired test to allow the instrument to warm up

Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results

Rinse 5 ml syringe with distilled water when finished

When grains are added in step 3d, color should be pink. If too many grains are added, color will
turn grayish purple
Questions? Please call 330-437-0067 for technical assistance

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44720

Instructions for: Nitrite

Method: Diazotization (Standard Methods)

Range: 0 0.4 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.)Scroll down to desired test and press enter. This will
allow the instrument to warm up while preparing the reagent test vial, thus saving time.
2. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
3. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested
to the vial
c. Recap and invert several times
d. Thoroughly wipe the vial clean
4. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
5. Press enter to continue and follow instructions on screen
6. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS

Select desired test to allow the instrument to warm up

Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results

Rinse 5 ml syringe with distilled water when finished

Questions? Please call 330-437-0067 for technical assistance

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44720

Instructions for: Orthophosphate High

Method: Vanadomolybdophosphoric (Standard Methods)

Acid Range: 0 50 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.)
2. Scroll down to desired test and press enter. This will allow the instrument to warm up while
preparing the reagent test vial, thus saving time.
3. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
4. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested
to the vial
c. Recap and invert several times
d. Thoroughly wipe the vial clean
e. Allow 1 0 minutes for reaction
5. Performing the test
a. Insert the cleaned blank vial into the curvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into curvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
f. Press enter to continue and follow instructions on screen
6. After completion of test, discard the reagent vial according to EPA regulations

IMPORTANT & HELPFUL STEPS

Select desired test to allow the instrument to warm up
Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results
Rinse 5 ml syringe with distilled water when finished
Be sure to allow 10 minutes in Step 4e
Questions? Please call 330-437-0067 for technical assistance

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44720

Instructions for: Orthophosphate Low

Method: Stannous Chloride (Standard Methods)

Range: 0 2 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.) Scroll down to desired test and press enter. This
will allow the instrument to warm up while preparing the reagent test vial, thus saving time.
2. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
3. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested
to the vial
c. Recap and invert 1 0 times
d. Uncap vial and carefully add exactly 1 drop of Orthophosphate Low reagent # 1
e. Recap and invert 10 times to mix
f. Thoroughly wipe the vial clean
4. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
5. Press enter to continue and follow instructions on screen
6. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS

Select desired test to allow the instrument to warm up

Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results

Rinse 5 ml syringe with distilled water when finished

Shelf life is no longer than 6 months

Positive interference may be caused by Silica and Arsenic if sample is heated

Negative interference may be caused by Arsenate, Fluoride, Sulfide, Thiosulfate and

Thiocyanate
Questions? Please call 330-437-0067 for technical assistance

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44720

Instructions for: Ozone

Method: DPD

Range: 0 5 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.) Scroll down to desired test and press enter. This
will allow the instrument to warm up while preparing the reagent test vial, thus saving time.
2. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
3. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested
to the vial
c. Recap and invert several times
d. Thoroughly wipe the vial clean
e. Allow 5 minutes for reaction
4. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
5. Press enter to continue and follow instructions on screen
6. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS
Select desired test to allow the instrument to warm up
Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results
Rinse 5 ml syringe with distilled water when finished
Be sure to allow 5 minutes for reaction in step 3e
Questions? Please call 330-437-0067 for technical assistance

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44720

Instructions for: pH
Method: Phenol Red (Standard Methods)

Range: 6.4 8.3

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.)
2. Scroll down to desired test and press enter. This will allow the instrument to warm up while
preparing the reagent test vial, thus saving time.
3. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
4. Preparing the reagent test vial (If Chlorine is present, follow Step 5)
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested to
the vial
c. Recap and invert several times
d. Thoroughly wipe the vial clean
5. Preparing the reagent test vial (Chlorine is present)
a. In a separate clean container, fill with 25 ml of the water to be tested
b. Carefully add exactly 4 drops from dropper bottle labeled Sodium Arsenite to the 25 ml
container and mix
c. Uncap one of the reagent test vials
d. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the 25 ml solution mixed
with Sodium Arsenite to the vial
e. Recap and invert several times
f. Thoroughly wipe the vial clean
g. Discard the remaining 20 ml Sodium Arsenite solution
6. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted,. insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
f. Press enter to continue and follow instructions on screen
7. After completion of test, discard the reagent vial according to EPA regulations

6657 Frank Avenue NW

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44720

Continued
Instructions for: pH
Method: Phenol Red Range: 6.4 8.3

IMPORTANT & HELPFUL STEPS

Select desired test to allow the instrument to warm up

Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results
Rinse 5 ml syringe with distilled water when finished

Questions? Please call 330-437-0067 for technical assistance

6657 Frank Avenue NW

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North Canton, Ohio
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44720

Instructions for: pH
Method: Methyl Blue (Standard Method)

Range: 7.6 9.6

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.) Scroll down to desired test and press enter. This
will allow the instrument to warm up while preparing the reagent test vial, thus saving time.
2. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean .
3. Preparing the reagent test vial (If Chlorine is present, follow Step 4)
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested
to the vial
c. Recap and invert several times
d. Thoroughly wipe the vial clean
e. Go to Step 5
4. Preparing the reagent test vial (only if Chlorine is present)
a. In a separate clean container, fill with 25 ml of the water to be tested
b. Carefully add exactly 4 drops from dropper bottle labeled Sodium Arsenite to the 25 ml
container and mix
c. Uncap one of the reagent test vials
d. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the 25 ml solution
mixed with Sodium Arsenite to the vial
e. Recap and invert several times
f. Thoroughly wipe the vial clean S
g. Discard the remaining 20 ml Sodium Arsenite solution
5. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
6. Press enter to continue and follow instructions on screen
7. After completion of test, discard the reagent vial according to EPA regulations

6657 Frank Avenue NW

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44720

Instructions for: pH

Page 2

IMPORTANT & HELPFUL STEPS

Select desired test to allow the instrument to warm up
Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results
Rinse 5 nil syringe with distilled water when finished
Questions? Please call 330-437-0067 for technical assistance

6657 Frank Avenue NW

Suite 200
North Canton, Ohio
www.hydrodynesystems.com

44720

Instructions for: Phenol

Method: Annoantipyrine (USEPA Approved)

Range: 0 5 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.) Scroll down to desired test and press enter. This
will allow the instrument to warm up while preparing the reagent test vial, thus saving time.
2. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
3. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested
to the vial
c. Recap and invert several times being sure reagent is completely dissolved
d. Thoroughly wipe the vial clean
4. Performing the test .
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
5. Press enter to continue and follow instructions on screen
6. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS
Select desired test to allow the instrument to warm up
Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results
Rinse 5 ml syringe with distilled water when finished
Questions? Please call 330-437-0067 for technical assistance

6657 Frank Avenue NW

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North Canton, Ohio
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44720

Instructions for: Phosphate Total

Method: Vanadomolybdophosphoric Acid (USEPA Approved)

Range: 0 50 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.) Scroll down to desired test and press enter. This
will allow the instrument to warm up while preparing the reagent test vial, thus saving time.
2. Preliminary Procedure
a. Place 50 ml of the water to be tested into an appropriately size glassware
b. Carefully add exactly 1 ml of 1 + 1 Normal Sulfuric Acid
c. Add the contents of 1 capsule labeled Ammonium Persulfate
d. Using a Hotplate I Stirrer slowly bring to a boil until a volume of 10 ml is left
e. Remove solution from heat when 10 ml is reached
f. While stirring, use a pH meter to adjust the pH of the 10 ml solutio n to 7.00 by adding I
drop of 1 Normal Sodium Hydroxide. Continue to add 1 drop at a time until pH of 7.00
g. After solution cools down, bring back to 50 ml by diluting with distilled water
h. Filter solution if it is not clear
3. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
4. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the solution from step
3 above to the vial
c. Recap and invert several times
d. Thoroughly wipe the vial clean
e. Allow 10 minutes for reaction
5. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
6. Press enter to continue and follow instructions on screen
7. After completion of test, discard the reagent vial according to EPA regulations

6657 Frank Avenue NW

Suite 200
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44720

Instructions for: Phosphate Total

Page 2

IMPORTANT & HELPFUL STEPS

Select desired test to allow the instrument to warm up
Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or Smudges from handling may affect
results
Rinse all glassware and 5 ml syringe with distilled water when finished
Positive interference is caused by Silica and Arsenic if heated
Negative interference is caused by Arsenate, Fluoride, Sulfide, Thiosulfate and Thiocyanate
Questions? Please call 330-437-0067 for technical assistance

6657 Frank Avenue NW

Suite 200
North Canton, Ohio
www.hydrodynesystems.com

44720

Instructions for: Potassium

Method: Tetraphenylboron

Range: 0 1O mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.) Scroll down to desired test and press enter. This
will allow the instrument to warm up while preparing the reagent test vial, thus saving time.
2. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
3. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested
to the vial
c. Recap and invert 20 times to mix
d. Uncap vial and carefully add exactly 3 drops of Reagent A
e. Recap and invert 20 times to mix
f. Thoroughly wipe the vial clean
g. Allow one minute for reaction
4. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening (Invert
reagent test vial 1 time immediately before inserting into cuvette)
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
5. Press enter to continue and follow instructions on screen
6. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS
Select desired test to allow the instrument to warm up
Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results
Rinse 5 ml syringe with distilled water when finished
Carefully add 3 drops of Reagent A in step 3d
Invert reagent test vial 1 time immediately before inserting into cuvette in step 4c
Questions? Please call 330-437-0067 for technical assistance

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44720

Instructions for: Silica High

Method: Colorimetric (Standard Methods)

Range: 0 100 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.) Scroll down to desired test and press enter. This
will allow the instrument to warm up while preparing the reagent test vial, thus saving time.
2. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
3. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested
to the vial
c. Carefully add exactly 2 drops of Reagent # 1 (Shake before using)
d. Recap and invert 5 times
e. Uncap vial and carefully add exactly 4 drops of Reagent # 2 (Shake before using)
f. Recap and invert 20 times to mix
g. Allow 5 minutes for reaction
h. Uncap vial and carefully add exactly 2 drops of Reagent #3 (Shake before using)
i. Recap and invert 20 times to mix
j. Thoroughly wipe the vial clean
k. Allow 2 minutes for reaction
4. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
5. Press enter to continue and follow instructions on screen
6. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS
Select desired test to allow the instrument to warm up
Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results
Rinse 5 ml syringe with distilled water when finished
Carefully add correct number of drops in steps 3c, 3e and 3h
Be sure to allow proper amount of time for reaction in steps 3g and 3k
Questions? Please call 330-437-0067 for technical assistance
6657 Frank Avenue NW
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44720

Instructions for: Silica Low

Method: Colorimetric (Standard Methods)

Range: 0 10 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.) Scroll down to desired test and press enter. This
will allow the instrument to warm up while preparing the reagent test vial, thus saving time.
2. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
3. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested
to the vial
c. Uncap vial and carefully add 1 spoonful (5 mg spoon) from bottle labeled Reagent # 1
d. Recap and invert 5 times
e. Allow 60 seconds for reaction
f. Uncap vial and carefully add 1 spoonful (5 mg spoon) from bottle labeled Reagent # 2
g. Recap and invert 60 times to mix
h. Thoroughly wipe the vial clean
i. Allow 10 minutes for reaction
4. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
5. Press enter to continue and follow instructions on screen
6. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS
Select desired test to allow the instrument to warm up
Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results
Rinse 5 ml syringe with distilled water when finished
Allow proper amount of times for reaction in steps 3e and 3i
Questions? Please call 330-437-0067 for technical assistance

6657 Frank Avenue NW

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North Canton, Ohio
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44720

Instructions for: Silver

Method: Dithizone (Standard Methods)

Range: 0 5 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.) Scroll down to desired test and press enter. This
will allow the instrument to warm up while preparing the reagent test vial, thus saving time.
2. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
3. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of Acetone (supplied) to
the vial
c. Recap and invert 20 times to mix
d. Rinse 5 ml syringe with distilled water before next step
e. Uncap vial and using 5 ml syringe, add 4 ml of the water to be tested to the vial
f. Recap and invert 20 times to mix
g. Uncap vial and carefully add exactly 3 drops of Silver reagent #2
h. Recap and invert 20 times to mix
i. Thoroughly wipe the vial clean
4. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
5. Press enter to continue and follow instructions on screen
6. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS
Select desired test to allow the instrument to warm up
Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results
Rinse 5 ml syringe with distilled water when finished
Be sure to add 5 ml in step 3b and 4 ml in step 3e
Carefully add exactly 3 drops of Silver reagent #2
Questions? Please call 330-437-0067 for technical assistance

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44720

Instructions for: Sulfate

Method: Turbidimetric (Standard Methods)

Range: 0 300 mg/L (ppm);

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.)
2. Scroll down to desired test and press enter. This will allow the instrument to warm up while
preparing the reagent test vial, thus saving time.
3. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water
to be tested
b. Thoroughly wipe the vial clean

4. Preparing the reagent test vial

a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be
tested to the vial
c. Recap and invert several times
d. Thoroughly wipe the vial clean
5. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
f. Press enter to continue and follow instructions on screen
6. After completion softest, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS

Select desired test to allow the instrument to warm up

Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping
clean with a dry paper towel. Foreign residue of any kind or

smudges from handling may affect results

Rinse 5 ml syringe with distilled water when finished

Questions? Please call 330-437-0067 for technical

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Instructions for: Sulfide

Method: Methylene Blue (USEPA Approved)

Range: 0 1.8 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.) Scroll down to desired test and press enter. This
will allow the instrument to warm up while preparing the reagent test vial, thus saving time.
2. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
3. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested
to the vial
c. Carefully add exactly I drop of Sulfide reagent # 2
d. Recap and invert 5 times
e. Allow 2 minutes for reaction
f. Uncap vial and carefully add exactly 3 drops of Sulfide reagent # 3
g. Recap and invert 5 times to mix
h. Thoroughly wipe the vial clean
4. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
5. Press enter to continue and follow instructions on screen
6. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS
Select desired test to allow the instrument to warm up
Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results
Rinse 5 ml syringe with distilled water when finished
Care must be taken to add correct number of drops in steps 3c and 3f
Questions? Please call 330-437-0067 for technical assistance

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Instructions for: Tannin & Lignin

Method: Colorimetric (Standard Methods)
Range: 0 7 mg/L (ppm)
1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.) Scroll down to desired test and press enter. This
will allow the instrument to warm up while preparing the reagent test vial, thus saving time.
2. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
3. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested
to the vial
c. Recap and invert 1 0 times to mix
d. Allow 1 minute for reaction
e. Uncap vial and carefully add exactly 20 drops of Reagent B
f. Recap and invert 10 times to mix
g. Thoroughly wipe the vial clean
h. Allow 5 minutes for reaction
4. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
5. Press enter to continue and follow instructions on screen
6. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS
Select desired test to allow the instrument to warm up
Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results
Rinse 5 ml syringe with distilled water when finished
Be sure to add correct amount of drops in step 3e
Interferences: Such reducing substances as 2 mg/LFerrous Iron and Sodium Sulfite individually
produce a color equivalent to 1 mg/L Tannic Acid. Sulfite Interferences can be removed by
adding 1 ml of Formaldehyde Solution to the water.
Questions? Please call 330-437-0067 for technical assistance

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Instructions for: Total Chrome

Method: 1,5 Diphenylcarbohydrazide (Standard Methods)

Range: 0 0.7 mg/L (ppm)

1. Preliminary Sample Treatment

a. Place 50 ml of water to be tested into appropriately sized glassware
b. Add 5 ml of Total Chrome reagent A (Use caution: contains 50 % Sulfuric Acid)
c. Add 1 ml of Total Chrome reagent B
d. Allow to sit 1 0 minutes for reaction
e. Using a Hotplate I Stirrer, boil sample until colorless
f. Allow to cool and then add distilled water to bring back to 50 ml or more
g. Bring solution to boil again and add drops from Total Chrome reagent C dropper bottle
until a faint pink color persists for 10 minutes while boiling
h. After pink color persists for 10 minutes of boiling, add drops from Total Chrome reagent
D dropper bottle until sample becomes colorless
i. Boil for an additional 2 minutes to remove any excess Azides
j. After sample cools down bring back to 50 ml by diluting with distilled water
2. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.) Scroll down to Hex Chrome test and press enter.
This will allow the instrument to warm up while preparing the reagent test vial, thus saving
time.
3. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
4. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the solution from Step
10 above to the vial
c. Recap and invert several times
d. Thoroughly wipe the vial clean
e. Allow ten minutes for reaction
5. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record as Total Chrome
6. Press enter to continue and follow instructions on screen
7. After completion of test, discard the reagent vial according to EPA regulations

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Instructions for: Total Chrome

Page 2

IMPORTANT & HELPFUL STEPS

Select desired test to allow the instrument to warm up
Be sure the outside of vial is thoroughly cleaned by dipping vial in water and wiping clean wit h
a dry paper towel. Foreign residue of any kind or smudges from handling may affect results
Rinse 5 ml syringe with distilled water when finished
Be sure to allow the required amount of time for reaction
Pink color in Step 1-g should remain after 10 minutes of boiling
Answer is to be recorded as Total Chrome (will display as Hex Chrome)
Use caution when performing procedure. Consult MSDS for proper care.
Questions? Please call 330-437-0067 for technical assistance

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Instructions for: Total Hardness

Method: EDTA

Range: 0 400 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.) Scroll down to desired test and press enter. This
will allow the instrument to warm up while preparing the reagent test vial, thus saving time.
2. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
3. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of distilled water to the
vial
c. Recap and invert several times
d. Uncap vial and using the 1 ml Syringe (supplied with Colorimeter) carefully add exactly
0. 1 ml of the water to be tested to the vial
e. Recap and invert 5 times to mix
f. Thoroughly wipe the vial clean .
g. Allow 30 seconds for reaction
4. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the cleaned reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
5. Press enter to continue and follow instructions on screen
6. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS
Select desired test to allow the instrument to warm up
Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results
Rinse 1 ml syringe with distilled water when finished
Must use distilled water in Step 3b
Be sure to add exactly 0. 1 ml of water to be tested in Step 3d
Questions? Please call 330-437-0067 for technical assistance

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Instructions for: Turbidity

Method: EPA I 80. 1 (USEPA Approved)
1. Before performing test, choose the desired
Range 0 1 then use
0.5
Range 0 - 100
then use
Range 0 - 1000
then use

Range: 0 I 1TU, 0 - 1 00 NTU, 0 800 NTU

range and standard to be tested.
NTU Standard
50
NTU Standard
200
NTU Standard

2. Mark two clean empty vials using a fine point permanent marker on the vial glass that is
exposed (when inserted into cuvette opening) slightly above the keypad. This will allow the user
to place the vial in the proper alignment each time in the cuvette opening of the Colorimeter.

3. Fill one marked empty vial with the proper NTU Standard and thorough ly wipe clean.
4. Choose the correct turbidity test on the Colorimeter and press enter. Please refer to General
Operating instructions for the Colorimeter for further information.)
5. Insert the filled standard vial into the Colorimeter taking special care t o align the mark on the
vial with the mark on the keypad next to the cuvette opening, place cover over vial and press
enter.
Important Note:
If performing the 0 1 range, then you must reuse the same vial the
standard is in for the water to be tested. Follow Step 6 to 8.
If performing 0 100 or 0 1000 range, then the standard vial may be kept for reuse with
other Turbidity tests. Follow Step 9 and 10.
6. Pour standard solution back into its original container for later use. Rinse the now empty vial
three or more times with distilled water.
7. Fill the cleaned vial with water to be tested, thoroughly wipe clean, insert into Colorimeter
taking special care align mark on the vial with mark on the keypad, place cover over vial and
press enter.
8. Read results on screen and record. Press enter to continue.
9. Fill second marked empty vial with the water to be tested, thoroughly wipe clean, insert into
Colorimeter taking special care to align the mark on the vial with the mark on the keypad, place
cover over the vial and press enter.
10. Read results displayed on screen and record. Press enter to continue.
IMPORTANT STEPS

Be sure the outside of the vial is thoroughly cleaned by dipping vial in water and wiping clean
with a dry paper towel. Foreign residue of any kind or smudges from handling may affect
results.

Take special care to properly align marks on vial and keypad.

0 1 range must use the same vial for standard and water to be tested a

When finished, rinse vials with distilled water thoroughly for reu se
Questions? Please call 330-437-0067 for technical assistance
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North Canton, Ohio
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44720

Instructions for: Zinc

Method: Zincon (USEPA Approved)

Range: 0 5 mg/L (ppm)

1. Press On/Enter key to power up the instrument. (Please refer to General Operating Instructions
for the Colorimeter for further instructions.) Scroll down to desired test and press enter. This
will allow the instrument to warm up while preparing the reagent test vial, thus saving time.
2. Preparing the blank vial
a. Select the uncapped empty vial (supplied with each test kit) and fill with the water to be
tested
b. Thoroughly wipe the vial clean
3. Preparing the reagent test vial
a. Uncap one of the reagent test vials
b. Use the 5 ml syringe (supplied with Colorimeter) and add 5 ml of the water to be tested
to the vial
c. Recap and invert several times
d. Uncap vial and carefully add exactly 5 drops of Zinc Reagent A
e. Recap and invert 5 times to mix
f. Thoroughly wipe the vial clean
g. Allow one minute for reaction
4. Performing the test
a. Insert the cleaned blank vial into the cuvette opening of the Colorimeter
b. Place the black cover over the vial and press enter
c. When prompted, insert the reagent test vial into cuvette opening
d. Place the black cover over the vial and press enter
e. Read the results displayed on the screen and record
5. Press enter to continue and follow instructions on screen
6. After completion of test, discard the reagent vial according to EPA regulations
IMPORTANT & HELPFUL STEPS
Select desired test to allow the instrument to warm up
Be sure the outside of the vial is thoroughly cleaned by dipping in water and wiping clean with a
dry paper towel. Foreign residue of any kind or Smudges from handling may affect results
Rinse 5 ml syringe with distilled water when finished
Carefully add exactly 5 drops of Reagent A
Questions? Please call 330-437-0067 for technical assistance

6657 Frank Avenue NW

Suite 200
North Canton, Ohio
www.hydrodynesystems.com

44720