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    Thin Layer Chromatography and Column Chromatography Results and Discussion

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    Jennifer Heredia
    This document summarizes chromatography experiments to separate and analyze the components of malunggay leaves. Column chromatography was used to separate colored components into 3 distinct eluates. Thin layer chromatography was then used to further separate the components, spotted on a plate and developed in a chamber. The distance traveled of each component from the origin was measured, allowing calculation of retention factor values. This provided data on component colors and distances traveled, allowing determination of purity based on the separation achieved through chromatography.

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    Thin Layer Chromatography and Column Chromatography Results and Discussion

    Uploaded by

    Jennifer Heredia
    3K views2 pages
    This document summarizes chromatography experiments to separate and analyze the components of malunggay leaves. Column chromatography was used to separate colored components into 3 distinct eluates. Thin layer chromatography was then used to further separate the components, spotted on a plate and developed in a chamber. The distance traveled of each component from the origin was measured, allowing calculation of retention factor values. This provided data on component colors and distances traveled, allowing determination of purity based on the separation achieved through chromatography.

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    results and discussion of chromatography experiment

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    This document summarizes chromatography experiments to separate and analyze the components of malunggay leaves. Column chromatography was used to separate colored components into 3 distinct eluates. Thin layer chromatography was then used to further separate the components, spotted on a plate and developed in a chamber. The distance traveled of each component from the origin was measured, allowing calculation of retention factor values. This provided data on component colors and distances traveled, allowing determination of purity based on the separation achieved through chromatography.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    3K views2 pages

    Thin Layer Chromatography and Column Chromatography Results and Discussion

    Uploaded by

    Jennifer Heredia
    This document summarizes chromatography experiments to separate and analyze the components of malunggay leaves. Column chromatography was used to separate colored components into 3 distinct eluates. Thin layer chromatography was then used to further separate the components, spotted on a plate and developed in a chamber. The distance traveled of each component from the origin was measured, allowing calculation of retention factor values. This provided data on component colors and distances traveled, allowing determination of purity based on the separation achieved through chromatography.

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    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 2

    RESULTS AND DISCUSSION

    Volume of Eluate

    Color of Component

    In this experiment, two types of chromatography

    (Drops)

    were used for the purpose of separation and

    1
    2
    3

    purity determination. Column chromatography,

    These eluates were then used as individual

    which runs a solvent that moves down slowly

    samples for spotting the TLC plates which were

    through a glass column with packed adsorbent,

    then put inside the developing chamber for

    was used to separate the colored components of

    completing

    malunggay leaves. On the other hand, thin layer

    Retention

    chromatography (TLC) which utilizes a stationary

    measuring the distance of each component from

    phase composed of a thin layer of adsorbent

    the origin and by dividing this to the solvent

    material on a glass plate or plastic sheet, was

    front, represented by the formula:

    based on solid-liquid chromatographic methods

    used

    to

    separate

    the

    components

    of

    each

    on

    the

    retention

    values

    (Rf

    values)

    obtained.
    Starting

    with

    separating
    malunggay

    the

    column

    chromatography

    for

    colored

    components

    the

    leaves,

    hexane-acetone

    of

    was

    with the same reagent, to be added next to


    perform the chromatography process.
    As the pure extract slowly moved down the
    column, 3.0 mL each of the solvents, specifically
    hexane-acetone, acetone, and acetone-methanol
    respectively, were added continously at the same
    time while collecting different colors of eluates in
    separate test tubes. For this, four differentcolored eluates were collected, of which two were
    mixed to provide three distinct-colored eluates,
    particularly, pale yellow, green, and yellow green.
    The following table presents the data collected
    under column chromatography.
    Table 1. Characteristic Color and Volume of
    Collected Eluate (Column Chromatography)

    were

    chromatography.

    then

    determined

    by

    Rf Value
    Distance of

    packed adsorbent without dissolving the solid.

    pure extract of malunggay leaves was prepared

    factors

    layer

    Component from Origin (X) with Determined

    be used as it run through the glass column with

    column to prevent it from running dry, as the

    thin

    Table 2. Characteristic Color and Distance of

    determined to be the suitable solvent system to

    This reagent was continuously added up on the

    the

    Distance of sample
    Distance of solvent
    Rf = origin origin

    collected eluate to overall determine their purity


    based

    Pale yellow
    Green
    Yellow Green

    1
    2
    3

    Color of

    component

    Component

    from origin (X)

    Pale Yellow
    Green
    Yellow Green

    in cm
    4.89 cm
    4.79 cm
    0.42 cm

    (attach

    explanation

    why

    Rf Value

    0.75
    0.74
    0.42

    retention

    factor

    is

    important for purity determination or how thin


    layer chromatograhy is related to the purity of
    the samplenow this explanation correlates with
    that

    of

    the

    chromatogram

    reuslts
    as

    seen

    obtained
    from

    from

    the

    the

    distance

    between each eluate, and as shown from the


    actual chromatogram presented below etc etc)

    Figure

    1.
    Figure 2. Dried Chromatogram with
    Markings

    Chromatogram under UV light

    In conclusion

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