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    Estimation of Glucose Concentration

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    Hansa Boricha
    This document describes a procedure to estimate the concentration of glucose in unknown solutions using a standard curve method with potassium permanganate. Students will prepare standard glucose solutions of known concentration and measure the time it takes for potassium permanganate solution to decolorize. A standard curve of concentration versus time will then allow students to estimate the concentration of glucose in three unknown solutions (A, B, C). The purpose is for students to gain experience using standard curves to quantify an unknown analyte concentration.

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    Estimation of Glucose Concentration

    Uploaded by

    Hansa Boricha
    574 views3 pages
    This document describes a procedure to estimate the concentration of glucose in unknown solutions using a standard curve method with potassium permanganate. Students will prepare standard glucose solutions of known concentration and measure the time it takes for potassium permanganate solution to decolorize. A standard curve of concentration versus time will then allow students to estimate the concentration of glucose in three unknown solutions (A, B, C). The purpose is for students to gain experience using standard curves to quantify an unknown analyte concentration.

    Original Description:

    Estimation of glucose using different reagents

    Original Title

    Estimation of glucose concentration

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    This document describes a procedure to estimate the concentration of glucose in unknown solutions using a standard curve method with potassium permanganate. Students will prepare standard glucose solutions of known concentration and measure the time it takes for potassium permanganate solution to decolorize. A standard curve of concentration versus time will then allow students to estimate the concentration of glucose in three unknown solutions (A, B, C). The purpose is for students to gain experience using standard curves to quantify an unknown analyte concentration.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    574 views3 pages

    Estimation of Glucose Concentration

    Uploaded by

    Hansa Boricha
    This document describes a procedure to estimate the concentration of glucose in unknown solutions using a standard curve method with potassium permanganate. Students will prepare standard glucose solutions of known concentration and measure the time it takes for potassium permanganate solution to decolorize. A standard curve of concentration versus time will then allow students to estimate the concentration of glucose in three unknown solutions (A, B, C). The purpose is for students to gain experience using standard curves to quantify an unknown analyte concentration.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 3

    Relation to topics / curriculum link:

    Biomolecules
    Food requirements

    Prior knowledge and skills needed:


    plotting of graph
    redox reaction
    Benedicts test

    Concept:
    Glucose is a reducing sugar. It has reducing power and can react with oxidising agents.

    Introduction
    Benedicts reagent is often used to test for the presence of reducing sugar (e.g. glucose) in
    solution by the formation of a brick-red precipitate. However, it is not very accurate
    when using Benedicts test to conduct quantitative analysis of glucose.
    Alternatively, potassium permanganate solution can be used to estimate the glucose
    content in a solution. Glucose reacts with the purple permanganate ions (MnO4-), and
    reduces to it into colourless manganese ions (Mn2+). The ionic equation for the reaction is
    shown below:
    MnO4- + 8H + + 5e- Mn2+ + 4H2O
    purple

    colourless

    The time taken for a standardised potassium permanganate solution to decolourise is


    related to the concentration of glucose in the solution. By measuring the time for the
    decolourisation of a standardised potassium permanganate solution, using a range of
    glucose solutions of known concentration, a standard curve can be prepared. Afterwards,
    the glucose concentration of an unknown solution can be estimated by looking up the
    standard curve.

    Estimation of glucose concentration

    Estimation of glucose concentration

    Materials

    Potassium permanganate solution (0.4 g in 1 litre distilled / deionised water)


    Sulphuric acid (1M)
    Glucose solution (10%)
    3 bottles of glucose solutions of unknown concentration (A, B, C)
    Stopwatch
    Glass rod
    Boiling tubes and test tube rack
    3 beakers
    3 pipettes and pipette fillers
    Some labels

    Activity: Estimation of glucose concentration of unknown solutions


    1.
    2.
    3.
    4.
    5.

    Use 10% glucose solution to prepare a series of dilutions of glucose solutions.


    Transfer 10 cm3 of one of the glucose solutions prepared into a boiling tube.
    Add 5 cm3 of sulphuric acid.
    Add 2 cm3 of potassium permanganate solution and start the stopwatch.
    Stir with a glass rod and record the time as soon as the purple colour disappears.

    6.

    Repeat (2) to (5) with other glucose solutions of known concentration until all are
    finished.
    7. Record your results, and share among the class.
    8. Calculate the class average results and record in a table.
    9. Plot the class average results on a graph paper to make a standard curve.
    10. Repeat (2) to (5) for glucose solutions (A, B and C) of unknown concentration.
    11. Use the standard curve to estimate the glucose concentration of solutions A, B and C.

    Questions for discussion


    1.
    2.
    3.

    What is the purpose of pooling the class results for plotting the standard curve?
    Suggest how you could improve the experiment.
    Suggest any possible reasons if your result is different from other groups.

    Further investigation
    Investigating the glucose content of a variety of health drinks
    Design an investigation to find out the glucose content of a variety of health drinks, and
    compare your results with the glucose content marked in the food labels. The results will be
    affected by the presence of vitamin C in some drinks, as it may cause the decolourisation of
    potassium permanganate solution to take place more quickly. Discuss the experimental
    design with your classmate before carrying out the investigation. Write a report of your
    investigation.

    Reference:
    National Centre for Biotechnology Education, (2006) Glucose Detector. Practical
    Biotechnology: A Guide for Schools and Colleges.

    Science and Plants for Schools (2007) Estimating glucose concentration in solution. SAPS.

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