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    Transport Media

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    sandalaila
    Transport media are formulated to preserve specimens from the time of collection until processing in the laboratory by minimizing bacterial overgrowth. Transport media vary depending on the suspected organisms but are generally classified as semi-solid or liquid, and as bacterial or viral transport media. Transport media contain buffers and salts to prevent microbial growth but no nutrients. Antibiotics or glycerol may be added. Commonly used transport media include Cary-Blair medium for Salmonella/Shigella detection, Amies medium with or without charcoal for various pathogens, Stuart's medium for gonorrhea, and viral transport medium for virus diagnosis.

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    Transport Media

    Uploaded by

    sandalaila
    256 views2 pages
    Transport media are formulated to preserve specimens from the time of collection until processing in the laboratory by minimizing bacterial overgrowth. Transport media vary depending on the suspected organisms but are generally classified as semi-solid or liquid, and as bacterial or viral transport media. Transport media contain buffers and salts to prevent microbial growth but no nutrients. Antibiotics or glycerol may be added. Commonly used transport media include Cary-Blair medium for Salmonella/Shigella detection, Amies medium with or without charcoal for various pathogens, Stuart's medium for gonorrhea, and viral transport medium for virus diagnosis.

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    microbiology,laboratory test,importance,types of transport media

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    Transport media are formulated to preserve specimens from the time of collection until processing in the laboratory by minimizing bacterial overgrowth. Transport media vary depending on the suspected organisms but are generally classified as semi-solid or liquid, and as bacterial or viral transport media. Transport media contain buffers and salts to prevent microbial growth but no nutrients. Antibiotics or glycerol may be added. Commonly used transport media include Cary-Blair medium for Salmonella/Shigella detection, Amies medium with or without charcoal for various pathogens, Stuart's medium for gonorrhea, and viral transport medium for virus diagnosis.

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    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    256 views2 pages

    Transport Media

    Uploaded by

    sandalaila
    Transport media are formulated to preserve specimens from the time of collection until processing in the laboratory by minimizing bacterial overgrowth. Transport media vary depending on the suspected organisms but are generally classified as semi-solid or liquid, and as bacterial or viral transport media. Transport media contain buffers and salts to prevent microbial growth but no nutrients. Antibiotics or glycerol may be added. Commonly used transport media include Cary-Blair medium for Salmonella/Shigella detection, Amies medium with or without charcoal for various pathogens, Stuart's medium for gonorrhea, and viral transport medium for virus diagnosis.

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    © All Rights Reserved
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    Download as docx, pdf, or txt
    of 2

    Transport media are special media formulated to preserve a specimen and minimize bacterial overgrowth from

    the time of collection to the time it is received at the laboratory to be processed. Depending on the type of
    organisms suspected in the sample, transport media may vary. However in general, transport media are
    classified on the basis of physical state as semi solid and liquid and also in basis of their utility as bacterial or
    viral transport media.

    What does a transport media contain?

    Contains only buffers and salt.

    Doesnt contain any nutritional ingredients such as carbon, nitrogen, and organic growth factors so as
    to prevent microbial multiplication.

    Addition of antibiotics and other substances like glycerol may be added for transporting specimens for
    tissue culture.

    What samples are collected in transport medium?


    All types of samples that may contain pathogens but could not be processed immediately requires transport
    medium. It may be Stool, urethral swabs, Nasal and throat swabs and specimens for tissue culture etc.

    What are the commonly used Transport mediums?


    Given below are some examples transport mediums with their purpose:

    1.

    Cary and Blair Medium: semi-solid, white colored transport medium for faeces that may
    contain Salmonella, Shigella, Vibrio or Campylobacter

    2.

    Amies medium with charcoal:Charcoal helps eliminate metabolic products of bacterial growth, which
    may be especially useful in the isolation of fastidious organisms like However it is suggested that,
    some other pathogens like Campylobacter can also survive in such medium.

    3.

    Amies medium without charcoal: Are ideal for the isolation of Mycoplasma and Ureaplasma

    4.

    Stuarts medium: Commonly used for transporting specimens suspected of having gonococci. Also
    used for transporting Throat, vaginal, wound and skin swabs that may contain fastidious organisms.

    5.

    Venkatraman Ramakrishnan (VR) medium: Used to transport feces from suspected cholera patients.

    6.

    Sachs buffered glycerol saline: Used to transport feces from patients suspected to be suffering from
    bacillary dysentery.

    7.

    Viral Transport Medium : This medium is ideal for diagnosis of viral infection. Ocular, respiratory and
    tissue swabs can be submitted in this medium. Fluid samples such as tracheal wash specimens or
    peritoneal fluid should be submitted as is, in sterile vials which prevent desiccation. In the absence of
    viral transport medium, submit swabs in sterile, sealed vials with several drops of saline added, to
    prevent desiccation. Cotton, plastic, wood-handled, and Dacron and other synthetic swabs are all
    acceptable. Calcium alginate swabs should be avoided. Bacterial transport medias are not appropriate
    for virology.

    8.

    Anaerobic Transport Medium (ATM): is a mineral salt base semi-solid media with reducing agents
    designed as a holding medium for maintaining viability of anaerobic bacteria It contains buffered
    mineral salts in a semi-solid media with sodium thioglycolate and cysteine added to provide a reduced
    environment. Resazurin may also be added as a redox indicator to reveal exposure to oxygen by
    turning pink. It provides an environment, which maintains viability of most microorganisms without
    significant multiplication and allows for dilution of inhibitors present in clinical material. Examples
    include thioglycolate broth

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