Bacteria Caracteristica S Epidemiologi A Diagnostico Virulencia Enfermedade S Vac, Trat Notas
Bacteria Caracteristica S Epidemiologi A Diagnostico Virulencia Enfermedade S Vac, Trat Notas
Bacteria Caracteristica S Epidemiologi A Diagnostico Virulencia Enfermedade S Vac, Trat Notas
Enterobacteri
aceae
a)Lactose
fermenters
E.coli,
Klebsiella,
Enterobacter
b)non lactose
fermenters
H2S
shigella,
serratia,
Yersernia,
Morganella,
Providencia
c)Non ferm
H2S +
Salmonella,
Proteus,
Citrobacter
Escherichia
coli
(coliforms)
Enterococci
Caracteristica
s
Lactose ferm
Epidemiologi
a
Normal flora of
GIT
O157 H7
Enterohemorr
Reservoir:
Diagnostico
Lactose +
Motile
IMViC ++- -
Virulencia
Somatic O
antigen (LPS)
Capsule K
Vi (capsular
antigen of
S.typhi)
Flagellar H
antigen
Fimbrial
antigens
Endotoxin:
-lipid A of LPS
-thermostable
-fever, shock
Enterotoxin:
cAMP or
cGMP
Cytotoxins,
bacteriocins
adhesins
Endotoxin,
colicins,
adhesins,
cytotoxin
(verotoxinhemorrhagic)
Enterotoxin
(plasmid)
Heat labile (LT
> cAMP,
cholera like)
Heat stable (ST
> cGMP)
Enfermedade
s
Vac, trat
Notas
Clinical samples:
Urine, feces,
CSF, blood,
biopsies,
sputum, pus
Quantitative
culture: urine
Media:
Enrichment,
differential,
selective
(MacConky)
Susceptibility
test
Antibiotics for
UTI/meningitis
Enteric disease:
Most are self
limiting
Prphylaxis for
ETEC: bismuth
subsalicylate
Antibiotics if
recommended
ANTIBIOTICS
ARE
Used to detect
fecal
contamination of
H2O
hagic E.coli
cattle,
vegetables,
non
pasteurized
fruit juices
Klebsiella
pneumonia
(Coliforme)
Serratia
marcescens
Non lactose
fermenter
H2S
Proteus
Non lactose
fermemter
H2S +
Salmonella sp
Salmon red
pigmented
colonies at
room temp
Multiple toxins
(shiga)
Most are
sorbitol
Acquired respi
flora in hospital
(nosocomial)
Lactose +
NON motile
IMViC - - ++
Opportunistic
pathogen of
respi
nosocomial
infect
Highly
resistant to
antibiotics
Most are
Swarming
motility
Rapid users of
urea with
ammonia
production (UTI
puede tener tb
piedras)
Motile
Capsule
Deaminate aa
(Phe)
e)EHEC- O157
H7
Severe diarrea
Hemolytic
uremic
sndrome (HUS)
-kidney
damage,
hemolytic
anemia,
thrombocytope
nia
Thrombocytope
nic purpura,
hemorrhagic
colitis
Pneumonia
with
hemorrhagic
necrotizing
consolidation of
the lungs
Dark red
bloody sputum
(currant jelly)
CONTRAINDICA
TED
risk of 2ry
complication
(HUS in
children)
Hydration
UTI
(nosocomial,
community)
Enterocolitis
O antigens
puede cross rxn
with rickettsiae
Gastroenteritis
Carriers
Non lactose
fermemter
H2S +
Shigella sp
Non lactose
fermenter
H2S
Anaerogenic
Highly virulent
zoonotic:
Poultry, cattle,
pets (turtle,
iguanas,
snakes)
NO K antigen
(capsule) but
S.typhi has Vi
(envelope)
(gastroenteritis
)
-(S.entiriditis)
(S.typhimurium
, most
common)
Enteric fever
(human only
reservoir)
-typhoid fever
(S.typhi)
-Paratyphoid
fever
(paratyphoid
strains)
Sepsis with
metastatic
infect
-S.cholerasuls
(osteomyelitis
in SCD pt)
-S. typhi,
S.paratyphi
(self limiting)
Gatorade
Yogurt
Avoid antibiotic
and lomotilor
immodium
Typhoid fever:
antibiotics
(chloramphenic
ol, ampicillin)
(gallbladder)
Only hosts:
human and
primates
Transmission: 6
Fs
Fingers, food,
fould H20,
fomites, flies,
STD
NON motile
NO capsule
Shiga toxin:
- enterotoxic
(V.cholera like)
-neurotox
(vascularity of
CNS)
-cytotox (inh
prot synthesis,
cleaves 60S)
Mild diarrheal
Bacilliary
dysentery
-large intestine
(erosion, ulcer)
-fever, cramps,
low volume
frequent stools,
blood, mucus in
feces
4 species:
S.dysenteriae
(Shiga bacillus)
-the most severe
disease, rare in
USA
S.flexneri,
S.boydii,
S.sonnei (most
common)
-less severe
Other Shigella
-sonnei and
flexneri
develop
antibodies that
neutralize
dysenteriae
toxin in vitro
Yersinia
Enterocolitica
Non lactose
fermenter
NON
FERMENTERS
Facultative
Grows best at
25C, CAN
GROW at 4C
Alkaline rxn in
TSI
AEROBES!
(some can use
NO3 as eacceptor)
Zoonotic
Food-borne
Direct contact
Intracell
parasite
Cytotoxic
Gastroenteritis
Mesenteric
lymphadenitis
Opportunistic,
ubiquitous
Oxidative +
Highly
resistant to
antimicrobials
Pseudomonas
Use organic
compounds
Grow 4C to 43C
Vegetables,
soil, whirlpools,
hot tubs
Hospital:
Respirators,
soaps
desinfectants,
water (tap,
bottled,
distilled)
Fomites
(telephones,
faucets)
Most are
oxidative +
Motile by polar
flagella
Biofilms in
plastic
Y.pseudotubercul
osis
-rare enteric
pathogen
Burkholderia
pseudomallei
(meliodiosis,
respi, SE Asia!)
B.mallei
(glanders
-respi disease:
horses, mules,
donkeys
-diff diseases in
humans)
Stenotrophomon
as maltophilia
(nosocomial,
chronic
infections)
Burkholderia
cepacia
(nosocomial,
CF )
Pseudomonas
aeruginosa
Only organism
to produce blue
pigment
pyocianin
Pyoverdin
(greenish
fluorescein)
Flora of the
skin and GIT
Oxidase +
Grape like odor
Several
pigments
(green
colonies)
Alcaligenes
sp ( =
Achromobact
er)
May be normal
flora
Nosocomial
-respirators
-sepsis,
pneumonia, ear
infections
Motile by
peritrichous
flagella
Endotoxin,
hemolysins
Retractable pili
Slime in strains
from cystic
fibrosis
patients
Exotoxin A
-diphteria like
(affects ADP
ribosylation of
EF-2, inh prot
synthesis)
UTI, meningitis,
ulcers,
swimmer;s ears
Pneumonia (CF
pt)
Abscesses (blugreen pus)
Hot-tub
folliculitis
(public baths)
Double
therapy:
penicillin and
aminoglycoside
LEADING CAUSE
OF DEATH IN
BURN PT: FEVER,
CELLULITIS,
SEPSIS
ECHTHYMA
GANGRENOSUM
Penicillin
except
M.catarrhalis
M.lacunata
(blepharoconjuntivitis)
M.osloensis
(osteoarthritis)
Neisseria-Like
coccobacilli
Moraxella sp
Normal flora of
mucous
membranes
Acinetobacter
baumanii
Normal flora
(GT, skin,
pharynx)
Nosocomial
(respi, UT)
Highly resistant
to
antimicrobials
Nosocomial
(faucets, ice,
water)
Previous
Flavobacteriu
m
Yellow colonies,
Weak
fermenter
Indole +
Meningitis,
Chryseobacte
rium
meningosepti
cum
sepsis of the
newborn
Adult:
pneumonia,
endocarditis,
sepsis,
meningitis, only
G bacillus S to
vancomycin
Other
Uncommon
Organism
Vibrionaceae
Curved bacilli
Vibrio
Comma shaped
bacilli
One polar
flagella
Most species
are halophilic
Classified by
the O antigen
Vibrio cholera
Capnocytophaga
gingivalis
Chromobacteriu
m violaceum
HACEK group
(oral flora,
associated to
endocarditis)
H.aprophilus
Actinobacillus
actinomycetemc
o
-mitans
Cardiobacterium
hominis
Eikenella
corrodens
Kingella kingae
Vibrio
Campylobacter
Helycobacter
Pleisomonas
Aeromonas
Oxidase +
No animal
reservoir,marin
Oxidase +
Resists alkaline
Cholera, other
food borne
diseases
Wound
infections
Sepsis
Cholera Enteric
Disease
Toxin causes
in cAMP and
01, 0139
(Benga) cause
Non halophilic!
e and surface
waters
(transmitted by
human fecal
contamination
of water and
food)
Endemic in
India and SE
Asia (Tx, Ls)
Vibrio
parahaemolyt
icus
Coastal marine
salt waters
(halophilic)
Vibrio
vulnificus
Halophilic
Contaminated
undercooked or
raw sea-food
(shellfish, raw
fish, sushi)
Trasnmitted
from wounds
by eating the
oysters
Campylobacte
r jejuni
Microaerophylic
grows at 42C
Zoonotic,
Normal flora on
many animals
pH
Selective TCBS
alkaline
medium for
isolation
Darting
motility
-heat labile
enterotoxin
(choleragen)
No fever,
profuse watery
diarrhea
No pus or blood
Vomiting,
dehydration,
loss of
electrolytes,
renal failure,
metabolic
acidosis and
death
efflux in ions
and water into
the small
intestine lumen
Treatment:
replace water
and electro to
prevent
hypovolemic
shock
Antibiot not
necessary but
reduce duration
of illness
After recovery
long lasting
immunity
Vaccines NOT
effective as an
epidemic
control
Mild to
explosive
diarrhea, fever,
vomiting
Self-limiting,
lasts a few
days
Gastroenteritis
(raw oysters)
Cellulitis,
hemorrhagic,
bullous lesions
Sepsis,
gangrene as a
result of
intrvascualr
coagulation
Gastritis,
colitis, diarrhea
with blood and
Doxycycline
Usually lasts a
week, selflinited
epidemic
disease
01 has 2
biotypes (El Tor,
Classic) and 3
serotypes
(Ogawa, Inaba,
Hikijima)
Non01/non0139
cause sporadic
disease or no
pathogenic
Assoc with
water, meat,
shellfish
Helicobacter
pylori
Microaerophilic
urease
Stomach of
45% adults,
also in some
animals
Gastric biopsy
Urea breath
test
H.pylori
antigens in
stool
IgG in serum
pus, enteric
fever
Guillain-Barre
may follow
Gastritis,
gastroduodenal
ulcers (risk
factor for
gastric
carcinoma)
Bismuth salts
Antibiot
(metronidazole)