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    Biology Practical Reports For Form 4 Experiment 8.11 (Practical Textbook Page 119)

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    ke2
    The document describes a biology experiment to study the effect of temperature on yeast activity. Yeast suspensions were placed in boiling tubes at different temperatures and the height of colored liquid in attached manometers was measured after 10 minutes. The results showed that yeast activity, indicated by liquid height, increased from 0°C to an optimum of 40°C then decreased at 50°C, supporting the hypothesis that yeast activity is optimal at 37°C.

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    Biology Practical Reports For Form 4 Experiment 8.11 (Practical Textbook Page 119)

    Uploaded by

    ke2
    91 views2 pages
    The document describes a biology experiment to study the effect of temperature on yeast activity. Yeast suspensions were placed in boiling tubes at different temperatures and the height of colored liquid in attached manometers was measured after 10 minutes. The results showed that yeast activity, indicated by liquid height, increased from 0°C to an optimum of 40°C then decreased at 50°C, supporting the hypothesis that yeast activity is optimal at 37°C.

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    Activity 8.11Teacher

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    The document describes a biology experiment to study the effect of temperature on yeast activity. Yeast suspensions were placed in boiling tubes at different temperatures and the height of colored liquid in attached manometers was measured after 10 minutes. The results showed that yeast activity, indicated by liquid height, increased from 0°C to an optimum of 40°C then decreased at 50°C, supporting the hypothesis that yeast activity is optimal at 37°C.

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    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    91 views2 pages

    Biology Practical Reports For Form 4 Experiment 8.11 (Practical Textbook Page 119)

    Uploaded by

    ke2
    The document describes a biology experiment to study the effect of temperature on yeast activity. Yeast suspensions were placed in boiling tubes at different temperatures and the height of colored liquid in attached manometers was measured after 10 minutes. The results showed that yeast activity, indicated by liquid height, increased from 0°C to an optimum of 40°C then decreased at 50°C, supporting the hypothesis that yeast activity is optimal at 37°C.

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    © All Rights Reserved
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    BIOLOGY PRACTICAL REPORTS FOR FORM 4

    Experiment 8.11 (practical textbook page 119)


    Problem statement: What is the effect of temperature on the activity of yeast?
    Objective : To study the effects of temperature on the activity of yeast.
    Hypothesis: The activity of yeast is optimal at 37oC.
    Variables: manipulated variable: temperature
    responding variable: height of the coloured liquid in the manometer
    constant variable:
    volume of yeast suspension / pH /light intensity / time taken
    Apparatus: boiling tubes, beakers, glass tubes, clips, rubber stoppers, rubber tubing, retort
    stands, manometer tubes, strings, measuring cylinders, stopwatches, thermometers
    Materials: yeast suspension (4g of yeast in 100 cm3 of glucose solution), coloured liquid, ice
    cubes
    Technique: record the level of coloured liquid in the manometer after 10 minutes.

    Apparatus set-up to study the effect of temperaure on the activity of yeast


    Procedure:
    1.
    2.
    3.
    4.
    5.
    6.
    7.
    8.
    9.

    Five boiling tubes A, B, C, D and E are labelled.


    All the boiling tubes are filled with 15 cm3 of yeast suspension.
    Five sets of the apparatus are set up.
    Boiling tube A is placed in a beaker of ice.The temperature is recoded after five minutes.
    The stopwatch is started and the level of coloured liquid in the manometer after 10 minutes
    is recorded.
    Step 4 is repeated by placing the boiling tubes , B, C, D and E in water baths at temperatures
    of 20oC, 30oC, 40oC and 50oC.
    The results are recorded in a table of results.
    A graph of the heights of the coloured liquid in the manometer against the temperatures is
    plotted.
    Safety precaution: The glucose solution has to be boiled to get rid of dissolved oxygen so
    that the yeast can respire anaerobically and produce ethanol and carbon dioxide.

    Results:
    Test tube
    A
    B
    C
    D
    E

    Temperature/ oC Height of the coloured


    liquid in the manometer/cm
    0
    -0.4
    20
    2.6
    30
    4.0
    40
    4.1
    50
    2.5

    Discussion:
    1. What do you observe in each boiling tube?
    In each boiling tube, the activity of yeast is shown by the foaming of the contents in the tube
    as well as the increased height of the coloured liquid.
    2. Give a reason for the observation in each boiling tube.
    As the yeast respires, the carbon dioxide that is released causes the pressure in the boiling
    tube to rise and subsequently pushes the coloured liquid up the manometer.
    3. What safety precautions do you need to take when conducting this experiment?
    The glucose solution has to be boiled to get rid of dissolved oxygen so that the yeast can
    respire anaerobically and produce ethanol and carbon dioxide.
    4. What is the optimum temperature for the activity of yeast? Give a reason.
    35 oC is the optimum temperature for the activity of yeast as the height of coloured liquid in
    the manometer is the highest, that is, 4.2 cm.
    Conclusion:
    Hypothesis is accepted.
    The activity of yeast is optimal at 37oC.

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