Hindawi Publishing Corporation

ISRN Nutrition
Volume 2013, Article ID 714970, 7 pages
http://dx.doi.org/10.5402/2013/714970

Research Article
Irregular Meal Timing Is Associated with Helicobacter pylori
Infection and Gastritis
Su-Lin Lim,1 Claudia Canavarro,2 Min-Htet Zaw,3 Feng Zhu,4 Wai-Chiong Loke,5
Yiong-Huak Chan,6 and Khay-Guan Yeoh4
1

Dietetics Department, National University Hospital, 5 Lower Kent Ridge Road, Main Building, Level 1, Singapore 119074
Dietetics and Nutrition Department, Alexandra Hospital, Jurong Health, Level 1, 378 Alexandra Road, Singapore 159964
3
Research and Strategic Planning Division, Research and Evaluation Department, Health Promotion Board,
3 Second Hospital Avenue, Singapore 168937
4
Department of Gastroenterology and Hepatology, National University Hospital, 5 Lower Kent Ridge Road Tower Block,
Level 10, Singapore 119074
5
Global Healthcare Practice, KPMG, 16 Raes Quay No. 22-00, Hong Leong Building, Singapore 048581
6
Biostatistics Unit, Clinical Research Centre, Yong Loo Lin School of Medicine, National University of Singapore,
Block MD 11, Level 1, Singapore 117597
2

Correspondence should be addressed to Su-Lin Lim; [email protected]

Received 7 November 2012; Accepted 9 December 2012
Academic Editors: M. S. Buchowski, Y.-H. Chen, and E. Devrim
Copyright 2013 Su-Lin Lim et al. is is an open access article distributed under the Creative Commons Attribution License,
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Helicobacter pylori (HP) is associated with chronic gastritis and gastric cancer, and more than half of the worlds population is
chronically infected. e aim of this retrospective study was to investigate whether an irregular meal pattern is associated with
increased risk of gastritis and HP infection. e study involved 323 subjects, divided into three groups as follows: subjects with HP
infection and gastritis, subjects with gastritis, and a control group. Subjects were interviewed on eating habits and meal timing. Multivariate logistic regression was used to compare groups. Adjusted odds ratios (OR) were derived controlling for gender, age, stress,
and probiotic consumption. Subjects who deviated from their regular meals by 2 hours or more had a signicantly higher incidence
of HP infection with gastritis (adjusted OR = 13.3; 95% CI 5.333.3; ) and gastritis (adjusted OR = 6.1; 95% CI 2.515.0;
). Subjects who deviated their meals by 2 hours or more, twice or more per week, had an adjusted OR of 6.3 and 3.5 of
acquiring HP infection with gastritis (95% CI 2.615.2; ) and gastritis (95% CI 1.58.5; ), respectively. Frequent
deviation in meal timing over a prolonged period appears associated with increased risk of developing HP infection and gastritis.

1. Introduction
Since the discovery of Helicobacter pylori (HP) in the 1980s,
considerable attention has been given to this bacterium as
a cause of gastritis and an established risk factor for gastric
cancer [13]. Helicobacter pylori is known to chronically
infect more than half of the worlds population [4]. Infection
is common in Singapore, aecting 71% of adults above 65
years and 3% of children below 5 years [5].
Helicobacter pylori infection is associated with a complex
interaction between genetic [6], socioeconomic [7], environmental [8], and bacterial factors [9]. is results in multiple
potential outcomes following infection, including chronic
gastritis and gastric adenocarcinoma [10, 11]. Due to the

close association between HP, gastritis and gastric cancer, it

is of interest to decrease the occurrence of HP infection and
gastritis.
To date, there is a scarcity of published literature on the
impact of irregular meals on HP infection or gastritis. is
study aims to determine whether a prolonged irregular meal
pattern is associated with increased risk of gastritis and HP
infection.

2. Materials and Methods

2.1. Ethics. e study protocol was approved by the National
Healthcare roup Domain Specic Review Board. Consent

ISRN Nutrition
Screening
(n = 340)

HP + gastritis group
128 subjects
successfully contacted.
7 subjects refused to
participate

Gastritis group
105 subjects
successfully contacted.
5 subjects refused to
participate

Control group

18 subjects with
normal gastroscopy
results (negative for
HP and gastritis).
All consented to
participate

89 subjects
successfully
contacted.
84 consented and met
the criteria for the
control group.
5 subjects had
symptoms/history of
HP or gastritis hence
were not included in
the study

Total subjects (n = 323):

121 with HP + gastritis, 100 with gastritis, 102 control

HP = Helicobacter pylori

F 1: Recruitment process.

was obtained from participants before the survey was carried

out.
2.2. Sample-Size Calculation. e sample size was calculated
based on a community survey of 113 people in Singapore
prior to the commencement of this study, which showed 16%
had irregular meals. Postulating that this prevalence would
double in subjects with HP and gastritis, 120 subjects per
group has a power of 80% and a 2-sided test of 5% to achieve
a statistically signicant result.

3. Subjects
All subjects were of Chinese ethnic origin and aged 50 years
and above, in order to minimize the confounding factors
of age and race. A total of 323 subjects were divided into
three groups according to HP and gastritis status. e HP
and gastritis group (Group A) consisted of patients diagnosed
with HP and gastritis ( ). e gastritis group (Group B)
consisted of patients who had been diagnosed with gastritis
but negative to HP ( ). All patients in Group
A and B had undergone endoscopic biopsy, with gastritis
and HP diagnosed from mucosal biopsy in three locations
(antrum, body, and cardia) and by consensus amongst three
pathologists according to the updated Sydney System for

the classication and grading of gastritis 12]. Subjects in

the control group (group C) had normal endoscopic biopsy
results ( ) or no symptoms or history of gastritis or
HP ( community-recruited subjects) ( ).
We compared the diet patterns of the 18 participants with
endoscopy results to the 84 without endoscopy and found
the patterns were similar ( much greater than 0.05 in all
parameters). Details of the recruitment process are described
in Figure 1.
3.1. Data Collection. All subjects were administered a specially designed questionnaire by two trained dietitians. is
included questions regarding regularity of meals, the frequency and duration of any changes to usual meal timing,
variation in the amount of food eaten, and the practice of
skipping meals. Subjects in Groups A and B were surveyed
regarding their eating patterns prior to the diagnosis of HP or
gastritis. Subjects in the control group (Group C) were asked
to respond regarding their eating pattern prior to endoscopy,
or prior to interview for the community recruited subjects.
We dened irregular meals as a deviation from regular
meal timing for 1 hour or more at least once per week.
Questions regarding the practice of skipping meals were
worded to detect subjects who omitted non-corresponding
meals of the day (i.e., not the same meal every day). Subjects
who missed the same meal each day were considered to

ISRN Nutrition

T 1: Demographics of study subjects.

HP + Gastritis
(Group A)
(n = 121)

Gastritis
(Group B)
(n = 100)

Control
(Group C) P value
(n = 102)

Mean SD
Range
Gender

62.5 7.3
5179

63.1 7.5
5287

61.0 7.0
5082

0.239

Male
Female

71 (58.7%)
50 (41.3%)

51 (51.0%)
49 (49.0%)

47 (46.1%)
55 (53.9%)

0.163

Age (year)

HP: Helicobacter pylori.

have a regular meal pattern consisting of one less meal per

day. e questionnaire also surveyed probiotic consumption,
the presence of stress or any major stressful event prior to
diagnosis, to enable these to be addressed as confounders.
3.2. Statistical Analysis. All analyses were performed using
SPSS 17.0 with statistical signicance set at .
Multivariate logistic regression was performed to determine
the risk predictors for the HP with gastritis and gastritis
groups. Unadjusted odds ratios were derived comparing
group A versus control (group C) and group B versus control
(group C) using chi-square or Fishers Exact test. Adjusted
odds ratios were derived controlling for gender, age, stress,
and consumption of probiotics.

4. Results
Table 1 describes the demographics of the study subjects.
ere were no signicant dierences in age and gender
distribution across the 3 study groups.
4.1. Deviation in Meal Timing. Table 2 shows that the
adjusted odds ratio (OR) of developing HP with gastritis
(Group A) and gastritis (Group B) increased as the time of
meal deviation increased. A deviation in meal timing of equal
to or more than 2 hours was associated with a signicant risk
of developing HP with gastritis or gastritis, with an adjusted
OR of 13.3 (95% CI 5.333.3, ) and 6.1 (95%
CI 2.515, ), respectively. e adjusted OR for
developing HP with gastritis and gastritis also increased as
the frequency of meal deviation increased (Table 3). Subjects
in Group A who deviated their meals equal to or more than
twice per week had an adjusted OR of 4.4 of developing HP
infection with gastritis (95% CI 2.38.7, ). ose
in Group B had an adjusted OR of 3.8 of developing gastritis
(95% CI 1.97.6, ).
Table 4 shows that subjects who deviated from their
regular meals by two or more hours, twice or more per week,
were associated with signicantly higher incidence of HP
infection with gastritis (adjusted OR = 6.3, 95% CI 2.615.2,
) and gastritis (adjusted OR = 3.5, 95% CI 1.58.5,
). ere were signicant dierences in the mean
period of meal deviation between the HP with gastritis,

gastritis and control groups (7.9 years versus 8.1 years versus
4.5 years, ) (Table 5).

4.2. Skipped Meals. Although the proportion of subjects

who skipped meals almost doubled in the HP with gastritis
and gastritis groups in comparison to those in the control
group (19% versus 9.8%), there was no signicant dierence
between the groups (Table 6).
4.3. Inconsistent Amount of Food Consumption. ere was no
signicant dierence between groups for subjects who had an
inconsistent amount of food at each meal (Table 7).

5. Discussion
is study is the rst to examine an association between the
degree of irregularity in meal timing and risk of HP and
gastritis. Aer controlling for the potential confounders of
gender, age, stress, and consumption of probiotics, we found
that deviating from regular meal timing by two hours or
more was associated with a thirteenfold increase in risk of
developing HP with gastritis, and a sixfold increase in risk
of developing gastritis.
e association of dietary habits with the development
of HP infection has been given relatively little attention.
A number of studies have demonstrated evidence of an
association between intake of specic food or nutrients and
HP [1316]. However fewer studies exist examining the
relationship between irregular meals and gastritis, and none
have studied the degree of irregularity in meal timing [17, 18].
A retrospective questionnaire study involving 76 men and 19
women with peptic ulcers in Japan found that eating irregular
meals signicantly increased the relative risk of peptic ulcer
in men, but not in women. In this instance the small number
of women subjects may not have provided enough power
for statistical signicance [17]. One Chinese study revealed
a signicant correlation between irregular meals and gastric
cardia cancer with an odds ratio of 4.2 [19]. However in
both studies, there was no mention how irregularity in meals
was surveyed, and whether deviation in meal timing, omitted
meals, and variations in food quantity were included.
Bulgarian researchers who found an increase in radiologically documented gastroduodenal ulcers during a period of
economic crisis reported their impression that skipped meals
and chain smoking were contributory factors [18]. e role
of traditional risk factors on the prevalence of duodenal ulcer
disease was investigated at an endoscopy unit in Jordan with
high prevalence of HP amongst patients. Skipping breakfast
or more than one meal was found to be among important
factors in the predisposition for ulcer disease in subjects with
HP [20].
In this study, meal regularity and habits prior to the
diagnosis of HP infection and gastritis were evaluated. e
odds ratio increased as the deviation in meal timing increased
in the case groups when compared to the control group. Not
everyone who is exposed to HP will become infected [21].
Before a person can be infected with HP, the bacteria must
penetrate the gastric mucosa [22]. e gastric mucosa acts as

ISRN Nutrition

T 2: Relationship between deviation from regular meals by number of hours and Helicobacter pylori infection with gastritis and gastritis.
Group A versus Control:
Odds Ratio
(95% Condence Interval)
P value
Unadjusted
Adjusted#

Deviation from
regular meals

Control
Count (%)

HP + Gastritis
(Group A)
Count (%)

Gastritis
(Group B)
Count (%)

0 to <1 hour

62 (60.8%)

38 (31.4%)

28 (28%)

OR = 1.0

OR = 1.0

1 to <1.5 hours

26 (25.5%)

24 (19.83%)

33 (33%)

OR = 1.5
(0.763.0)
P = 0.242

OR = 1.5
(0.743.2)
P = 0.249

1.5 to <2 hours

5 (4.9%)

11 (9.09%)

8 (8%)

OR = 3.6
(1.211.1)
P = 0.027

OR = 4.2
(1.314.1)
P = 0.019

2 hours

9 (8.8%)

48 (39.67%)

31 (31%)

OR = 8.7
(3.819.7)
P < 0.001

OR = 13.3
(5.333.3)
P < 0.001

HP: Helicobacter pylori, OR: Odds ratio.

#
Adjusted for gender, age, stress, and use of probiotics.

Statistical signicance.
Note: there were no statistical dierences in the frequency of deviation from regular meals between groups A and B.

Group B versus Control:

Odds Ratio
(95% Condence Interval)
P value
Unadjusted
Adjusted#
OR = 1.0
OR = 1.0
OR = 2.8
(1.45.6)
P = 0.003
OR = 3.5
(1.111.8)
P = 0.039

OR = 7.6
(3.218.1)
P < 0.001

OR = 2.5
(1.25.0)
P = 0.014

OR = 3.3
(0.9611.6)
P = 0.058
OR = 6.1
(2.515.0)
P < 0.001

T 3: Relationship between deviation from regular meals by frequency per week and Helicobacter pylori infection with gastritis and
gastritis.

Frequency of meal
deviation per week

Control
Count (%)

HP + Gastritis
(Group A)
Count (%)

62 (60.8%)

38 (31.4%)

Gastritis
(Group B)
Count (%)
28 (28%)

15 (14.7%)

25 (20.66%)

21 (21%)

25 (24.51%)

58 (47.93%)

51 (51%)

Group A versus Control:

Odds Ratio
(95% Condence Interval)
P value
Unadjusted
Adjusted
OR = 1.0

OR = 1.0

OR = 2.7
(1.35.8)
P = 0.01

OR = 2.9
(1.36.5)
P = 0.008

OR = 3.8
(2.07.0)
P < 0.001

HP: Helicobacter pylori, OR: Odds ratio.

#
Adjusted for gender, age, stress, and use of probiotics.

Statistical signicance.
Note: there were no statistical dierences in frequency of meal deviation between groups A and B.

a natural protective barrier, which limits the penetration of

microorganisms [23]. However we do not yet know whether
irregularity in meal timing changes the mucosal membrane
and increases susceptibility to bacterial penetration. It has
recently been established that the barrier function of the
mucosa can be disturbed under a variety of pathological
insults [24]. We hypothesize that people who have irregular
meals are at higher risk of HP infection or gastritis because
during the usual meal timing the stomach and intestines
produce secretions, free radical scavengers or perhaps some
other yet to be discovered chemical, in readiness to receive
food. If food is not ingested during this time, the secretions
or lack of secretions somehow cause the lining of the stomach
to be susceptible to HP infection and gastritis. It has been
established that HP survives in brief exposure to acidic pH
of less than 4 and growth occurs only at the relatively narrow

OR = 4.4
(2.38.7)
P < 0.001

Group B versus Control:

Odds Ratio
(95% Condence Interval)
P value
Unadjusted
Adjusted
OR
= 1.0
OR = 1.0
OR = 3.1
(1.406.89)
P = 0.006
OR = 4.52
(1.111.8)
P < 0.001

OR = 2.7
(1.16.2)
P = 0.023

OR = 3.8
(1.97.6)
P < 0.001

pH range of 5.5 to 8.0, with optimal growth at neutral pH [25,

26]. Upon entry to the host, spiral morphology and agellar
motility facilitates penetration of the more pH neutral viscous
mucosal layer for infection to occur [27, 28]. is could be
indicative of a possible eect of meal timing deviation on the
gastric pH that makes the mucosa susceptible to HP infection.
e mean duration of meal timing deviation in this study
was about 8 years for the case groups compared to 4.5 years
in the control group. It has been suggested that to cause
harm, HP must eciently adapt to the gastric niche, a process
that takes place over many years and involves regulation of
bacterial genes in response to environmental factors [29].
ese environmental factors may include, but are not limited
to, cigarette smoking, stress, irregularity in meal timing and
other dietary factors. Stress has been shown to increase
gastric permeability to pathogens such as HP [30, 31].

ISRN Nutrition

T 4: Distribution and odds ratio for subjects who deviate from regular meals stratied by hours and frequency of meal deviation.
Subjects who deviated from their regular meals by
1 hour for 2 times per week

Control (n = 102)

Count (%)

Unadjusted
Odds Ratio
(95% CI)
P value

Adjusted#
Odds Ratio
(95% CI)
P value

25 (24.5%)

OR = 1

OR = 1.0

HP + Gastritis
(Group A) (n = 121)

58 (47.9%)

Gastritis (Group B)
(n = 100)

51 (51%)

Subjects who deviated from their regular meals by

2 hours for 2 times per week
Adjusted#
Unadjusted
Odds Ratio
Odds Ratio
(95% CI)
Count (%)
(95% CI)
P value
P value

OR = 3.1
(1.75.8)
P < 0.001

OR = 2.8
(1.65.0)
P < 0.001

OR = 2.9
(1.55.3)
P = 0.001

OR = 3.2
(1.85.8)
P < 0.001

8 (7.8%)
34 (28%)

26 (26%)

HP: Helicobacter pylori, OR: Odds ratio.

#
Adjusted for gender, age, stress, and use of probiotics.

Statistical signicance.
Note: there were no statistical dierences in frequency of meal deviation between groups A and B.

T 5: Mean period of meal deviation habit for Helicobacter pylori

with gastritis, gastritis, and control group.

Control
(n = 102)
HP + Gastritis
(Group A)
(n = 121)
Gastritis
(Group B)
(n = 100)
Total (n = 323)

Mean SD
(years)
4.5 6.7
7.9 7.1
8.1 7.2

6.9 7.2

95% Condence
Interval for Mean

6.69.1
6.79.6

P < 0.001

6.17.7

HP: Helicobacter pylori.

Statistical signicance.

It is well established that HP infection leads to gastritis

[32]. Although our study showed a signicant association
between irregular meal timing and gastritis as well as
occurrence of HP infection, it did not determine whether
irregularity in meal timing is the cause or eect of these.
We postulate that frequent deviation from regular timing of
meals is likely to cause gastritis or HP infection. Glutathione
level has been found to be elevated in HP infection and some
forms of gastritis [33, 34]. Irregularity in meal timing possibly
increases glutathione production in the stomach. In addition
it may also cause low gastric acid secretion and studies have
shown that clinical conditions with low gastric acid secretion
are associated with increased risk of gastric cancer [35, 36].
Meal timing may also impact physiological parameters such
as endocrine variables [37]. In our study, the signicant
dierences in the regularity of meal timing of the HP with
gastritis and gastritis groups in comparison to the control
group supports the presence of the above mechanisms.

OR = 1.0

OR = 4.6
(2.010.5)
P < 0.001

OR = 6.3
(2.615.2)
P < 0.001

OR = 4.1
(1.89.6)
P = 0.001

OR = 3.5
(1.58.5)
P = 0.005

T 6: Distribution and odds ratio for subjects who regularly

skipped meals.
Omitted meal at
least one time per
week
Count (%)

Unadjusted
Odds ratio
(95% CI)
P value

Adjusted#
Odds ratio
(95% CI)
P value

Control
(n = 102)

10 (9.8%)

OR = 1

OR = 1

HP + Gastritis
(Group A)
(n = 121)

23 (19%)

OR = 2.2
(0.984.8)
P = 0.058

OR = 2.3
(0.995.5)
P = 0.053

Gastritis
(Group B)
(n = 100)

19 (19%)

OR = 2.2
(0.954.9)
P = 0.067

OR = 2.2
(0.95.3)
P = 0.094

Dierence
between
groups

3.25.8

OR = 1

HP: Helicobacter pylori, OR: Odds ratio.

#
Adjusted for gender, age, stress, and use of probiotics.
Note: there were no statistical dierences in frequency of skipped meals
between groups A and B.

e merits of this study are the fairly large sample

size and the use of endoscopic biopsy as endpoint for
diagnosis in three quarters of the study population. Due
to ethical issues, endoscopic biopsies were not carried out
on the community-recruited subjects in the control arm.
However, comparison of participants with and without
endoscopy results showed similar diet patterns and baseline
characteristics (analyses not shown in this paper). Some
subjects in the control group may have had HP without
their knowledge, as individuals may remain asymptomatic
despite having HP [38]. Active chronic gastritis may also
not present any clinical symptoms [27]. If we had been able
to denitively eclude HP or gastritis in these communityrecruited subjects it may have further strengthened the results
of this study, as 39% of subjects in the control group had
irregular meals.

ISRN Nutrition

T 7: Distribution and odds ratio for subjects who had an

inconsistent amount of food for each meal.
Inconsistent
amount of food in
corresponding
meals
Count (%)

Unadjusted
Odds ratio
(95% CI)
P value

Adjusted#
Odds ratio
(95% CI)
P value

9 (8.8%)

OR = 1

OR = 1.0

18 (14.9%)

OR = 1.8
(0.774.2)
P = 0.172

OR = 1.8
(0.84.4)
P = 0.177

OR = 2.6
(1.16.0)
P < 0.027

OR = 2.1
(0.85.3)
P = 0.095

Control
(n = 102)
HP + Gastritis
(Group A)
(n = 121)
Gastritis
(Group B)
(n = 100)

20 (20%)

HP: Helicobacter pylori, OR: Odds ratio.

#
Adjusted for gender, age, stress, and use of probiotics use.

Statistical signicance.
Note: there were no statistical dierences in the frequency of inconsistent
amount of food between groups A and B.

A major limitation of this study was the retrospective

design and its inability to provide causal link of HP infections and gastritis to irregular eating patterns. Surveyor and
respondent bias were further limitations in this retrospective
study. Signicant recall bias was possible with the selfreported questionnaires. An individual with chronic gastritis
or HP infection might likely be much more aware of their
dietary habits than a healthy control. In addition, the dietitians administering the questionnaire were not blinded to the
participants diagnosis.

6. Conclusion
In conclusion, a variation in meal timing over a prolonged
period appears to be associated with increased risk of symptomatic HP infection and gastritis. Regular timing of meals
may play an important role in the prevention of these two
medical conditions. As there is a scarcity of published data
studying an association between irregular meal timing and
HP and gastritis, this pilot paper warrants future prospective
studies to determine the eect of irregular meals on the
development of gastritis and HP.

Authors Contribution
S. L. Lim conceptualized the research design, planned the
study, participated in the data collection, and wrote the
paper. C. Canavarro participated in the data collection and
commented on the paper. M. H. Zaw was involved in
the interpretation of results and revision of the paper. F.
Zhu participated in the recruitment of the participants and
commented on the paper. W. C. Loke provided signicant
advice and was involved in revision of the paper. Y. H. Chan
analyzed and interpreted the results of the study. K. G. Yeoh
provided signicant advice, was involved in the research
design, and commented on the paper.

Acknowledgments
e authors wish to thank the dietitians of the Dietetics
Department, National University Hospital, Singapore, for
their assistance with community-based data collection prior
to the study.

References
[1] P. Grbel, L. Huang, N. Masubuchi, F. J. Stutzenberger, and D.
R. Cave, Detection of Helicobacter pylori DNA in houseies
(Musca domestica) on three continents, e Lancet, vol. 352,
no. 9130, pp. 788789, 1998.
[2] G. D. Eslick, L. L. Y. Lim, J. E. Byles, H. H. X. Xia, and N. J.
Talley, Association of Helicobacter pylori infection with gastric
carcinoma: a meta-analysis, American Journal of Gastroenterology, vol. 94, no. 9, pp. 23732379, 1999.
[3] B. J. Marshall and J. R. Warren, Unidentied curved bacilli in
the stomach of patients with gastritis and peptic ulceration, e
Lancet, vol. 1, no. 8390, pp. 13111314, 1984.
[4] J. Salgueiro, M. Zubillaga, C. Goldman et al., Review article: is there a link between micronutrient malnutrition and
Helicobacter pylori infection? Alimentary Pharmacology and
erapeutics, vol. 20, no. 10, pp. 10291034, 2004.
[5] K. M. Fock, Helicobacter pylori infectioncurrent status in
Singapore, Annals of the Academy of Medicine Singapore, vol.
26, no. 5, pp. 637641, 1997.
[6] F. C. Tseng, E. E. Brown, E. M. Maiese et al., Polymorphisms in
cytokine genes and risk of Helicobacter pylori infection among
Jamaican children, Helicobacter, vol. 11, no. 5, pp. 425430,
2006.
[7] G. I. Perez-Perez, D. Rothenbacher, and H. Brenner, Epidemiology of Helicobacter pylori infection, Helicobacter, vol. 9,
supplement 1, pp. 16, 2004.
[8] L. M. Brown, T. L. omas, J. L. Ma et al., Helicobacter
pylori infection in rural China: demographic, lifestyle and
environmental factors, International Journal of Epidemiology,
vol. 31, no. 3, pp. 638646, 2002.
[9] J. G. Fox, P. Correa, N. S. Taylor et al., High prevalence and
persistence of cytotoxin-positive Helicobacter pylori strains in a
population with high prevalence of atrophic gastritis, American
Journal of Gastroenterology, vol. 87, no. 11, pp. 15541560, 1992.
[10] M. N. Week, L. Gao, and H. Brenner, Helicobacter pylori
infection and chronic atrophic gastritis, Epidemiology, vol. 20,
no. 4, pp. 569574, 2009.
[11] H. Brenner, V. Arndt, C. Stegmaier, H. Ziegler, and D.
Rothenbacher, Is Helicobacter pylori infection a necessary
condition for noncardia gastric cancer? American Journal of
Epidemiology, vol. 159, no. 3, pp. 252258, 2004.
[12] M. F. Dixon, R. M. Genta, J. H. Yardley et al., Classication
and grading of Gastritis: the updated Sydney system, American
Journal of Surgical Pathology, vol. 20, no. 10, pp. 11611181,
1996.
[13] M. Jarosz, E. Rychlik, H. Siuba et al., Dietary and socioeconomic factors in relation to Helicobacter pylori re-infection,
World Journal of Gastroenterology, vol. 15, no. 9, pp. 11191125,
2009.
[14] M. Gotteland, M. Andrews, M. Toledo et al., Modulation
of Helicobacter pylori colonization with cranberry juice and
Lactobacillus johnsonii La1 in children, Nutrition, vol. 24, no.
5, pp. 421426, 2008.

ISRN Nutrition
[15] Y. Y. Lee, A. W. Ismail, N. Mustaa et al., Sociocultural and
dietary practices among Malay subjects in the north-eastern
region of Peninsular Malaysia: a region of low prevalence of
Helicobacter pylori infection, Helicobacter, vol. 17, no. 1, pp.
5461, 2012.
[16] S. Cruchet, M. C. Obregon, G. Salazar, E. Diaz, and M. Gotteland, Eect of the ingestion of a dietary product containing
Lactobacillus johnsonii La1 on Helicobacter pylori colonization
in children, Nutrition, vol. 19, no. 9, pp. 716721, 2003.
[17] H. Yashiro, A. Higashi, N. Miyaji et al., A comparative study of
psycho-social factors in peptic ulcer disease, Japanese Journal
of Gastroenterology, vol. 91, no. 6, pp. 10751085, 1994.
[18] P. Pomakov, S. Gueorgieva, J. Stantcheva, T. Tenev, and A.
Rizov, Peptic ulcers during periods of acute economical crisis,
Journal de Radiologie, vol. 74, no. 5, pp. 265267, 1993.
[19] L. Cai, Z. L. Zheng, and Z. F. Zhang, Risk factors for the gastric
cardia cancer: a case-control study in Fujian Province, World
Journal of Gastroenterology, vol. 9, no. 2, pp. 214218, 2003.
[20] N. A. Abu Farsakh, Risk factors for duodenal ulcer disease,
Saudi Medical Journal, vol. 23, no. 2, pp. 168172, 2002.
[21] D. Y. Graham, A. R. Opekun, M. S. Osato et al., Challenge
model for Helicobacter pylori infection in human volunteers,
Gut, vol. 53, no. 9, pp. 12351243, 2004.
[22] S. M. El-Shoura, Helicobacter pylori: I. Ultrastructural
sequences of adherence, attachment, and penetration into the
gastric mucosa, Ultrastructural Pathology, vol. 19, no. 4, pp.
323333, 1995.
[23] R. Sharma, C. Young, and J. Neu, Molecular modulation of
intestinal epithelial barrier: contribution of microbiota, Journal
of Biomedicine and Biotechnology, vol. 2010, Article ID 305879,
14 pages, 2010.
[24] K. Mukojima, S. Mishima, J. Oda et al., Protective eects
of free radical scavenger edaravone against xanthine oxidasemediated permeability increases in human intestinal epithelial
cell monolayer, Journal of Burn Care and Research, vol. 30, no.
2, pp. 335340, 2009.
[25] J. J. E. Bijlsma, M. Lie-A-Ling, I. C. Nootenboom, C. M. J.
E. Vandenbroucke-Grauls, and J. G. Kusters, Identication of
loci essential for the growth of Helicobacter pylori under acidic
conditions, Journal of Infectious Diseases, vol. 182, no. 5, pp.
15661569, 2000.
[26] J. J. E. Bijlsma, B. Waidner, A. H. M. Van Vliet et al., e
Helicobacter pylori homologue of the ferric uptake regulator is
involved in acid resistance, Infection and Immunity, vol. 70, no.
2, pp. 606611, 2002.
[27] J. G. Kusters, A. H. M. Van Vliet, and E. J. Kuipers, Pathogenesis
of Helicobacter pylori infection, Clinical Microbiology Reviews,
vol. 19, no. 3, pp. 449490, 2006.
[28] K. M. Ottemann and A. C. Lowenthal, Helicobacter pylori uses
motility for initial colonization and to attain robust infection,
Infection and Immunity, vol. 70, no. 4, pp. 19841990, 2002.
[29] N. De Vries, E. J. Kuipers, N. E. Kramer et al., Identication of
environmental stress-regulated genes in Helicobacter pylori by a
lacZ reporter gene fusion system, Helicobacter, vol. 6, no. 4, pp.
300309, 2001.
[30] S. Levenstein, Stress and peptic ulcer: life beyond helicobacter,
British Medical Journal, vol. 316, no. 7130, pp. 538541, 1998.
[31] S. Nakane and T. Sakai, Relationship between development
of stress ulcer and dye permeability of the gastric mucosa
during stress, Folia Pharmacologica Japonica, vol. 69, no. 5, pp.
673678, 1973.

7
[32] P. Correa, e epidemiology and pathogenesis of chronic
gastritis: three etiologic entites, Frontiers of Gastrointestinal
Research, vol. 6, pp. 98108, 1980.
[33] F. Farinati, G. D. Libera, R. Cardin et al., Gastric antioxidant,
nitrites, and mucosal lipoperoxidation in chronic gastritis and
Helicobacter pylori infection, Journal of Clinical Gastroenterology, vol. 22, no. 4, pp. 275281, 1996.
[34] I. Beno, K. Volkovov, M. Btovsky, and M. Staruchov,
Increased mucosal antioxidant enzyme activities in chronic
gastritis and benign gastric polyps, European Journal of Cancer
Prevention, vol. 2, no. 6, pp. 461465, 1993.
[35] K. Iijima, S. Ohara, T. Koike, H. Sekine, and T. Shimosegawa,
Gastric acid secretion of normal Japanese subjects in relation to
Helicobacter pylori infection, aging, and gender, Scandinavian
Journal of Gastroenterology, vol. 39, no. 8, pp. 709716, 2004.
[36] S. Imagawa, M. Ito, M. Yoshihara, H. Eguchi, S. Tanaka, and K.
Chayama, Helicobacter pylori dupA and gastric acid secretion
are negatively associated with gastric cancer development,
Journal of Medical Microbiology, vol. 59, no. 12, pp. 14841489,
2010.
[37] U. Holmbck, A. Lowden, T. kerfeldt et al., e human body
may buer small dierences in meal size and timing during
a 24-h wake period provided energy balance is maintained,
Journal of Nutrition, vol. 133, no. 9, pp. 27482755, 2003.
[38] S. Lanciers, B. Hauser, Y. Vandenplas, and U. Blecker, e
prevalence of Helicobacter pylori positivity in asymptomatic
children of dierent ethnic backgrounds living in the same
country, Ethnicity and Health, vol. 1, no. 2, pp. 169173, 1996.

MEDIATORS
of

INFLAMMATION

The Scientific
World Journal
Hindawi Publishing Corporation
http://www.hindawi.com

Volume 2014

Gastroenterology
Research and Practice
Hindawi Publishing Corporation
http://www.hindawi.com

Volume 2014

Journal of

Hindawi Publishing Corporation

http://www.hindawi.com

Diabetes Research
Volume 2014

Hindawi Publishing Corporation

http://www.hindawi.com

Volume 2014

Hindawi Publishing Corporation

http://www.hindawi.com

Volume 2014

International Journal of

Journal of

Endocrinology

Immunology Research
Hindawi Publishing Corporation
http://www.hindawi.com

Disease Markers

Hindawi Publishing Corporation

http://www.hindawi.com

Volume 2014

Volume 2014

Submit your manuscripts at

http://www.hindawi.com
BioMed
Research International

PPAR Research
Hindawi Publishing Corporation
http://www.hindawi.com

Hindawi Publishing Corporation

http://www.hindawi.com

Volume 2014

Volume 2014

Journal of

Obesity

Journal of

Ophthalmology
Hindawi Publishing Corporation
http://www.hindawi.com

Volume 2014

Evidence-Based
Complementary and
Alternative Medicine

Stem Cells
International
Hindawi Publishing Corporation
http://www.hindawi.com

Volume 2014

Hindawi Publishing Corporation

http://www.hindawi.com

Volume 2014

Journal of

Oncology
Hindawi Publishing Corporation
http://www.hindawi.com

Volume 2014

Hindawi Publishing Corporation

http://www.hindawi.com

Volume 2014

Parkinsons
Disease

Computational and
Mathematical Methods
in Medicine
Hindawi Publishing Corporation
http://www.hindawi.com

Volume 2014

AIDS

Behavioural
Neurology
Hindawi Publishing Corporation
http://www.hindawi.com

Research and Treatment

Volume 2014

Hindawi Publishing Corporation

http://www.hindawi.com

Volume 2014

Hindawi Publishing Corporation

http://www.hindawi.com

Volume 2014

Oxidative Medicine and

Cellular Longevity
Hindawi Publishing Corporation
http://www.hindawi.com

Volume 2014