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Corn Genetics Lab

This document provides instructions for students to conduct a chi square analysis on data from a monohybrid and dihybrid corn cross. Students are asked to collect data on kernel color and texture from their corn sample and perform chi square calculations to determine if their observed ratios match the expected Mendelian ratios. They are also asked to consider what could cause deviations from the expected ratios and conclude whether their data supports the given parental genotypes.

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Abhi A
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990 views4 pages

Corn Genetics Lab

This document provides instructions for students to conduct a chi square analysis on data from a monohybrid and dihybrid corn cross. Students are asked to collect data on kernel color and texture from their corn sample and perform chi square calculations to determine if their observed ratios match the expected Mendelian ratios. They are also asked to consider what could cause deviations from the expected ratios and conclude whether their data supports the given parental genotypes.

Uploaded by

Abhi A
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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CORN GENETICS CHI SQUARE ANALYSIS

Name_______________________________

Monohybrid Cross
1. Count the number of purple and yellow kernels in five of the
rows on your ear of corn and record the number on the chart.
Be sure to use the same five rows for each calculation.
2. Count the number of smooth and shrunken seeds on the
same five rows and record on the chart .

!
TABLE 1

Number of Kernels

Kernel Coloration

Kernel Percentage (divide


count by total)

1. Which phenotypes appear to be dominant?

2. What are the probable genotypes of the parents with


regard to coloration? (Use P)

Purple
Yellow
Total (for 5 rows)
Kernel Texture

3. What are the probable genotypes of the parents with


regard to texture? (Use S)

Smooth
Shrunken

4. What is the expected % of purple to yellow?

Total (for 5 rows)

5. Does your observed data match what is expected?

3. Now count the number of each in your five rows on the ear of corn.
TABLE 2

Number Counted

Percent: Number counted / total * 100

Purple & smooth


Purple & shrunken
Yellow & smooth
Yellow & shrunken
TOTAL

4. Complete the dihybrid cross (#6 below and at the top of the next page). Stop after #7 then complete #5 below.
5. Now look at Table 2: did you obtain a 9:3:3:1 ratio? ________ If you did not, then the genes may be found on the
same chromosome and do not assort independently. To determine if the deviations from your observed data are due
to chance alone or if the data is significantly different, you need to use a chi square test. Proceed to #8.

Dihybrid Cross
6. We will now consider a dihybrid cross, which is a combination of the two monohybrids. Your ear of corn may be a
result of a cross between plants that were both heterozygous for color and texture (PpSs x PpSs). Work out this cross
in the Punnet square below.

P: ____________ x _____________

7. Calculate the phenotypic ratios for each type of seed based on the punnett square above.
Purple & smooth _______________

Yellow & smooth _______________

Purple & shrunken ______________

Yellow & shrunken ______________

8. First calculate the expected number you should have gotten based on your total number assuming a 9:3:3:1 ratio.
Calculate the individual chi square values for each row and add them all together to determine your overall chi square
value.
Expected Number
Purple & smooth

Total x 9/16 =

Purple & shrunken

Total x 3/16 =

Yellow & smooth

Total x 3/16 =

Yellow & shrunken

Total x 1/16 =

Observed Number

expected

CHI SQUARE VALUE ========>


(add the numbers from the rows above)

9. Now determine if your chi square value is a good fit with your data. Your degrees of freedom (df) is the number of
possible phenotypes minus 1. In your case, 4 - 1 = 3. Find the number in that row that is closest to your chi square
value. Circle that number.
10. Explain what it means to have a "good fit" or a "poor fit". Does you chi square analysis of real corn data support
the hypothesis that the parental generation was PpSs x PpSs?

11. List two reasons why data might have a


poor chi square fit?

PROBLEM SET
Chi Square Problem Set
1. Problem: A large ear of corn has a total of 433 grains, including 271 Purple & starchy, 73 Purple & sweet, 63
Yellow & starchy, and 26 Yellow & sweet.
Your Tentative Hypothesis: This ear of corn was produced by a dihybrid cross
(PpSs x PpSs) involving two pairs of heterozygous genes resulting in a theoretical (expected) ratio of 9:3:3:1.
Objective: Test your hypothesis using chi square and probability values.

2. Problem: In a certain reptile, eyes can be either black or yellow. Two black eyed lizards are crossed, and the result
is 72 black eyed lizards, and 28 yellow-eyed lizards.
Your Tentative Hypothesis: The black eyed parents were Bb x Bb.
Objective: Test your hypothesis using chi square analysis. In this set, because only two values (traits) are examined,
the degrees of freedom (df) is 1. SHOW ALL WORK!

3. Problem: A sample of mice (all from the same parents) shows


58 Black hair, black eyes 16 Black hair, red eyes
19 White hair, black eyes 7 White hair, red eyes
Your tentative hypothesis: (what are the parents?)

Objective: Use a chi square analysis to support your hypothesis

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