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Ilagan, Myzhel S. Inumerable, and Natasha R. Jain Group 5 2C-Pharmacy Organic Chemistry Laboratory

The document summarizes an experiment to prepare buffer solutions with different pH levels and use liquid indicators to determine the pH of the buffers and samples colorimetrically. The experiment involved preparing a phosphate buffer at pH 7.00 using phosphoric acid and sodium hydroxide according to calculated volumes. Color standards were created by adding liquid indicators to prepared buffer solutions. The pH of samples like distilled water, shampoo, and orange juice were then determined by adding indicators and observing the color changes. The results and discussions section describes the preparation of the buffer, color standards, and pH determination of the samples.

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64 views2 pages

Ilagan, Myzhel S. Inumerable, and Natasha R. Jain Group 5 2C-Pharmacy Organic Chemistry Laboratory

The document summarizes an experiment to prepare buffer solutions with different pH levels and use liquid indicators to determine the pH of the buffers and samples colorimetrically. The experiment involved preparing a phosphate buffer at pH 7.00 using phosphoric acid and sodium hydroxide according to calculated volumes. Color standards were created by adding liquid indicators to prepared buffer solutions. The pH of samples like distilled water, shampoo, and orange juice were then determined by adding indicators and observing the color changes. The results and discussions section describes the preparation of the buffer, color standards, and pH determination of the samples.

Uploaded by

Myzhel Inumerable
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© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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pH MEASUREMENT AND BUFFER PREPARATION

Christelle Mae S. Guy, Bea Armanie U. Haboc, Hannah Marie G. Herrella, Isabelle Louise F.
Ilagan, Myzhel S. Inumerable, and Natasha R. Jain
Group 5
2CPharmacy
Organic Chemistry Laboratory
ABSTRACT
The experiment was conducted to prepare different buffer solutions and determine the pH of the buffers and samples
colorimetrically using different liquid indicators.

INTRODUCTION
Yeyeyeyeyey

+0.62 n

HP O4 -2

1.0000 n

EXPERIMENTAL

H 2 P O 4-

1.62 n

A. Compounds tested
15M phosphoric acid (H 3PO4), 6M sodium
hydroxide (NaOH), distilled water (H2O),
Eight O Clock orange juice, Sunsilk Brilliant
Shine
shampoo,
acid-base
indicators
(thymol
blue,
bromophenol
blue,
bromocresol green, bromocresol purple,
phenol
red,
methyl
orange,
phenolphthalein)
B. Procedure
1. Preparation of Buffer
The buffer solution was prepared using
the following guidelines:
Table 1. Guidelines for buffer solution
preparation
Weak acid

H2PO4-

Conjugate base
Volume
Concentration
pH
pKa

HPO4-2
250 mL
0.100 M
7.00
7.21

(salt)
(acid)

pH = pKa + log

(HPO4 -2 )
( H 2 P O 4- )
-2
(HPO4 )
7.0 7.21 = log
( H2 P O4 )

Actual number of moles of phosphate buffer

250 mL

0.1 n
0.250 L =0.025 n
L
Actual number of moles of

0.6 2 n/L
(HPO4 -2 )
=
1.0000 n/L
( H 2 P O 4- )
Theoretical number of moles

HP O42

-2
0. 62 n n HP O4
=
1.6 2 n
0.025 n

HPO4-2 = 0.095 n
Actual number of moles of


H 2 P O4

1.0000 n n H 2 P O4
=
1.6 2 n
0.025 n

H2PO4- = 0.0154 n
mL of H3PO4 needed

0.025 n

7.0 = 7.21+ log

antilog(-0.21) = 0.6166

1L
=0.250 L
1000 mL

1L
1000 mL

=1. 4 7 mL H3 P O4
17 n 1 L

mL of 6 M NaOH
0.025 n + 0.010 n = 0.035 n

0.04 n

1 L 1000 mL

=6 . 67 mL NaOH
6n 1L

Amount of distilled water needed


250 mL = 1.67 mL + 5.83 mL + x
250 mL 9.7361 mL = 242.5 mL distilled H2O

A volume of 1.67 mL ( _ drops) 15M


phosphoric acid (H3PO4) and 5.83 mL ( _ drops)
of 6M NaOH was aspirated using a serological
pipette while _ mL of distilled water was
measured in a graduated cylinder. The reagents
were mixed in an Erlenmeyer flask. The buffer
was transferred in an amber-colored bottle with
label.
2. Preparation of color standards using
the buffer solutions
5 mL of prepared buffers was pipetted
into 8 test tubes labeled properly with the
corresponding acid-base indicator to be
added. 2 drops of a liquid indicator were
placed into a test tube. The procedure was
performed using the other indicators. The
color changes were taken down.
3. Determination of the pH of samples
5 mL of the sample (distilled water,
Sunsilk Brilliant Shine shampoo, and Eight
OClock orange juice) was measured using
a graduated cylinder and were transferred
into a test tube with label of the sample to
be tested. 2 drops of an acid-base indicator
was added and the change in the color was
noted down. The procedure was repeated
using a different indicator.

RESULTS AND DISCUSSIONS


Preparation of the Buffer
Preparation of color standards using the
buffer solutions
Determination of the pH of samples

REFERENCES
1. Troy, David. (2006). Remington: The
Science and Practice of Pharmacy 21st
Edition. Philadelphia. Lippincott
William and Wilkins.(pp.499-500)
2. Cecil, J.R. (1995). Basic Biochemical
Laboratory Procedures and Computing
with Principles, Review Questions,
Worked Examples, and Spreadsheet
Solutions. (1st ed.). New York: Oxford
University Press. Pages 40-65.
3.

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