Scribd Logo
Upload

Welcome to Scribd!

  • 204 views

    Fingerprinting Indonesia Cacao Clones PDF

    Uploaded by

    Nur Insana Imaniar
    This document summarizes research to identify off-type (misidentified) trees in cocoa clones in Indonesia. The objectives were to more accurately identify parental clones for breeding to increase cocoa yields and disease resistance. Researchers extracted DNA from over 4,000 trees and genotyped them using SNP markers. They found 5 trees (4.42%) were off-types among 113 trees from 13 clones. True-to-type parental trees identified will be used in future pollination and flowering trials, and data will be shared publicly to help cocoa breeding and farmers. Collaboration between research institutions is integrating genotyping into studying and correcting cocoa germplasm collections.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Fingerprinting Indonesia Cacao Clones PDF

    Uploaded by

    Nur Insana Imaniar
    204 views1 page
    This document summarizes research to identify off-type (misidentified) trees in cocoa clones in Indonesia. The objectives were to more accurately identify parental clones for breeding to increase cocoa yields and disease resistance. Researchers extracted DNA from over 4,000 trees and genotyped them using SNP markers. They found 5 trees (4.42%) were off-types among 113 trees from 13 clones. True-to-type parental trees identified will be used in future pollination and flowering trials, and data will be shared publicly to help cocoa breeding and farmers. Collaboration between research institutions is integrating genotyping into studying and correcting cocoa germplasm collections.

    Original Title

    Fingerprinting Indonesia Cacao Clones.pdf

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    This document summarizes research to identify off-type (misidentified) trees in cocoa clones in Indonesia. The objectives were to more accurately identify parental clones for breeding to increase cocoa yields and disease resistance. Researchers extracted DNA from over 4,000 trees and genotyped them using SNP markers. They found 5 trees (4.42%) were off-types among 113 trees from 13 clones. True-to-type parental trees identified will be used in future pollination and flowering trials, and data will be shared publicly to help cocoa breeding and farmers. Collaboration between research institutions is integrating genotyping into studying and correcting cocoa germplasm collections.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    204 views1 page

    Fingerprinting Indonesia Cacao Clones PDF

    Uploaded by

    Nur Insana Imaniar
    This document summarizes research to identify off-type (misidentified) trees in cocoa clones in Indonesia. The objectives were to more accurately identify parental clones for breeding to increase cocoa yields and disease resistance. Researchers extracted DNA from over 4,000 trees and genotyped them using SNP markers. They found 5 trees (4.42%) were off-types among 113 trees from 13 clones. True-to-type parental trees identified will be used in future pollination and flowering trials, and data will be shared publicly to help cocoa breeding and farmers. Collaboration between research institutions is integrating genotyping into studying and correcting cocoa germplasm collections.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 1

    Off-types Identification in Fingerprinting Indonesia Cocoa Clones

    Rany Agustina Susanti*, Juan Carlos Motamayor, Donald Livingstone III**, Smilja Lambert***
    *Mars Indonesia, Makassar Indonesia
    **Mars-USDA, Miami United States
    *** Mars Australia, Ballarat Australia

    4. Result
    1. The Problem
    Indonesia has problem with low cocoa yield and pests / diseases
    Breeding and selection process for superior cocoa clones needs long time and big
    efforts
    Cocoa germplasm collections are badly maintained with many mis-identification
    (off-types)
    Need to identify best and right individual trees (true-to-type) to be used as
    parental clones

    MRCC Parental Trees Summary


    Total sample : 13 clones, 113 trees
    Off-types trees : 5 trees (4.42% from total trees)
    Off-types trees has been found in PBC123, M05, and BB01 clones

    5. Follow up research
    All the true-to-type parental trees will be used for pollination and
    flowering trial research

    Data is shared publications

    Mars Makassar, Indonesia


    DNA Lab

    Mars Miami DNA Lab

    2. Objectives
    To have faster and more accurate breeding and selection process for
    producing new, high yielding and resistant cocoa clones
    To identify the right individual trees to be used as parental clones (trueto-type)
    To complement phenotypic data with genetic information for higher
    accuracy and to speed up the selection process
    Data is shared in public domain to help cocoa breeder and farmers

    This work is being supported by the Cocoa Genome Project where Mars Inc. have
    sequenced and annotated the whole cocoa genome and release data in the public
    domain to be accessible to all cocoa scientists on the World to use it for developing
    better cocoa varieties for cocoa farmers around the world. In 2010, the results were
    placed on the Web by the intellectual property organization Public Intellectual
    Property Resource for Agriculture (PIPRA) which helps developing countries gain
    access to agricultural science they can use. Any scientist can access the genome
    after registering on the website. By making the results publicly available, scientists
    will have access to key learnings to advance plant science, while plant breeders and
    farmers around the world will be able to develop cacao trees that are higher yielding
    and resistant to Pests/diseases, this way assuring sustainable cocoa production into
    the future.

    3. Method

    DNA Extraction
    1. Samples collecting in the field
    2. Cutting and homogenizing leaf sample
    3. Centrifuge to take supernatant
    4. Binding DNA with binding matrix
    5. Eluting DNA with pure water

    http://www.cacaogenomedb.org/

    SNP method
    (Large amount sample, shorter time)
    Using Dapeng 24 Markers (Mat_150, Mat_Tc1060,

    Mat_Tc75, Mat_Tc151,
    Mat_Tc139,
    Mat_Tc1126, Mat_Tc364, Mat_Tc90, Mat_Tc230, Mat_Tc891, Mat_Tc1439, Mat_Tc591, Mat_Tc174,
    Mat_Tc534, Mat_Tc1484, Mat_Tc372, Mat_Tc998, Mat_Tc329, Mat_Tc702, Mat_Tc144, Mat_Tc602,
    Mat_Tc899, Mat_Tc226, Mat_Tc878)

    Fluidigm Technology

    http://cocoasustainability.com/

    Laboratory Activities

    Summary
    We were able to extract DNA and genotype more than 4033 cocoa trees from
    Indonesia (from Mars Regional Cocoa Center research station, Luwu South
    Sulawesi) in less than 6 months
    We succeeded in identifying and defining a strategy that allowed us to quantify
    and
    normalize large amount of DNA samples in short time
    We succeeded in identifying the leading laboratories which used the latest
    technologies and in defining a strategy that allowed us to genotype
    Collaboration between research institutes are now integrated in studying and
    correcting individual trees of cocoa germplasm collection in the field

    You might also like