As Biology Revision L3 Cells Microscopes and IAM PPQ 2

Tupton Hall School
Q1.The photograph shows part of the cytoplasm of a cell.
(a)
(i)
Organelle X is a mitochondrion.
What is the function of this organelle?
...............................................................................................................
...............................................................................................................
(1)
(ii)
Name organelle Y.
...............................................................................................................
(1)
(b)
This photograph was taken using a transmission electron microscope. The structure
of the organelles visible in the photograph could not have been seen using an
optical(light) microscope. Explain why.
........................................................................................................................
........................................................................................................................
........................................................................................................................
........................................................................................................................
........................................................................................................................
(2)
(Total 4 marks)
Page 1
Tupton Hall School
Q2.
An amoeba is a single-celled, eukaryotic organism. Scientists used a transmission

electron microscope to study an amoeba. The diagram shows its structure.
(a)
(i)
Name organelle Y.
...............................................................................................................
(1)
(ii)
Name two other structures in the diagram which show that the amoeba is a
eukaryotic cell.
1 ............................................................................................................
2 ............................................................................................................
(2)
(b)
What is the function of organelle Z?

........................................................................................................................
........................................................................................................................
........................................................................................................................
(1)
(c)
The scientists used a transmission electron microscope to study the structure of the
amoeba. Explain why.
(2)
(Total 6 marks)
Page 2
Tupton Hall School
Q3.
The drawing shows an electron micrograph of parts of epithelial cells from the small
intestine.
(a)
(i)
Name the structures labelled A.

.............................................................................................................
(1)
(ii)
Explain how these structures help in the absorption of substances from the
small intestine.
.............................................................................................................
.............................................................................................................
(1)
(b)
(i)
The scale bar on this drawing represents a length of 0.1m. Calculate the
magnification of the drawing. Show your working.
Magnification .............................................
(2)
(ii)
Explain why an electron microscope shows more detail of cell structure than a
light microscope.
.............................................................................................................
.............................................................................................................
.............................................................................................................
(2)
Page 3
Tupton Hall School
(Total 6 marks)
Page 4
Tupton Hall School
M1.(a)
(i)
(Aerobic) respiration;
Accept ATP production / energy release
Reject anaerobic respiration
Reject energy production
1
(ii)
Golgi (apparatus / body);

Ignore smooth ER
1
(It = Optical microscope)

Ignore reference to magnification
(b)
1.
Has low resolution / not high enough resolution;

Accept converse relating to EM
2.
(Because) wavelength of light not short enough / too long;

Accept larger wavelength
Accept statements that microscopes have a wavelength
2
[4]
M2.
(a)
(i)
Golgi (apparatus / body);

1
(ii)
1.
Nucleus;
1. Accept: nucleolus / nuclear envelope / nuclear
membranes
2.
Mitochondrion;
2. Accept cristae / mitochondrial membranes
3.
Endoplasmic reticulum / ER;

3. Ignore reference to rough / smooth
4.
Lysosome;
4. Reject lysozyme
2 max
Page 5
Tupton Hall School
(b)
(Aerobic) respiration / ATP production / provide energy;

Accept Krebs cycle / electron transport.
Ignore 'produces energy'
Reject anaerobic respiration
Ignore what energy is used for
1
(c)
1.
High / better resolution;
2.
Shorter wavelength;
3.
To see internal structures / organelles / named organelles;

3. Accept ultrastructure
2 max
[6]
M3.
(a)
(i)
microvilli; (reject brush border)

1
(ii)
increased surface area (for diffusion);

1
(b)
(i)
principle of
(15 17 tolerance)
160000;
(correct answer award 2 marks)
2
(ii)
electron microscope has a greater resolving

power / objects closer
together can be distinguished;
electron (beams) have a shorter wavelength;
2
(c)
short diffusion pathway /short pathway to the centre / large SA:V ratio
for faster, more diffusion;
1
[7]
Page 6
Tupton Hall School
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Tupton Hall School

Q1.The photograph shows part of the cytoplasm of a cell.

Tupton Hall School

An amoeba is a single-celled, eukaryotic organism. Scientists used a transmission

What is the function of organelle Z?

Tupton Hall School

Name the structures labelled A.

Tupton Hall School

Tupton Hall School

Golgi (apparatus / body);

(It = Optical microscope)

Has low resolution / not high enough resolution;

(Because) wavelength of light not short enough / too long;

Golgi (apparatus / body);

Endoplasmic reticulum / ER;

Tupton Hall School

(Aerobic) respiration / ATP production / provide energy;

High / better resolution;

To see internal structures / organelles / named organelles;

microvilli; (reject brush border)

increased surface area (for diffusion);

electron microscope has a greater resolving

Tupton Hall School

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