IICA / CIRAD-EMVT Questionnaire, June 1998
QUESTIONNAIRE ON VETERINARY DIAGNOSTIC LABORATORY
CAPABILITIES IN THE CARIBBEAN
___________________________________________________________________
COUNTRY : DATE :
NAME OF LABORATORY
RESPONDENT Name
Position in the laboratory
Address
Telephone
Fax
E-mail
___________________________________________________________________
I - GENERAL ORGANISATION OF THE LABORATORY
Address of the laboratory :
Location Isolated
Headquarters of veterinary services
Attached to a university
Attached to an abattoir
Attached to another laboratory
Other
The building organization (number of units...)
Please attach a schematic diagram with surface areas and function of the rooms on a separate sheet.
Cooperation with other laboratories Yes No
List the other laboratories in the country their diagnostic capability
- -
- -
- -
- -
The laboratory is administered by which agency or organisation :
Annual budget (*This information will remain confidential)
Salaries : % of total budget :
Operating costs : % of total budget :
Government contribution : % of total budget :
Cost recovery : % of total budget :
Other resources : % of total budget :
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IICA / CIRAD-EMVT Questionnaire, June 1998
II - DESCRIPTION OF THE TECHNICAL UNITS
SIZE
(m)
TECHNIQUES USED
LIST THE MAIN TESTS/ PATHOGENS / RESI
(where applicable)
AVG NO ANALYSES / YR
(for each path./residue)
NECROPSY
-
-
-
-
-
-
-
-
-
-
-
-
CLINICAL CHEMISTRY
1 Urinalysis
2 - Other
-
-
-
-
-
-
-
-
-
-
-
-
HEMATOLOGY
1 Blood smears
2 - CBC & Differential
3 - Coagulation profile
4 - Other
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
HISTOPATHOLOGY
1 - Paraffin embedding
& section
2 - Cryosection
3 - Staining
4 - Immunostaining
5 Other
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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IICA / CIRAD-EMVT Questionnaire, June 1998
SIZE
(m)
TECHNIQUES USED
List them
LIST THE MAIN PATHOGENS / RESIDUES
(where applicable)
AVG NO ANALYSES / YR
(for each path./residue)
BACTERIOLOGY
1 - Isolation
2 - Identification
3 Antibiotic sensitivity
4 - Typing
5 - Other
-
-
-
-
-
-
-
-
-
-
VIROLOGY
1 - Isolation
2 - Identification
3 - Typing
-
-
-
-
-
-
MYCOLOGY
1 - Isolation
2 - Identification
3 - Sensitivity to drugs
4 - Other
-
-
-
-
-
-
-
-
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IICA / CIRAD-EMVT Questionnaire, June 1998
SIZE
(m)
TECHNIQUES USED
List them
LIST THE MAIN PATHOGENS / RESIDUES
(where applicable)
AVG NO ANALYSES / YR
(for each path./residue)
PARASITOLOGY
- Ectoparasites
- Helminths
- Hemoparasites
- Protozoa
1 - Identification
2 - Sensitivity to drugs
1 - Identification
3 - Sensitivity to drugs
1 - Blood smears
2 - Microcentrifugation
3 - Heartworm test
1 - Identification
-
-
-
-
-
-
-
-
-
-
-
-
BIOTECHNOLOGY
- Serology
- Detection of antigens
- Detection of DNA
1 - Agglutination
2 - Immunofluorescence
3 - ELISA
1 - ELISA
2 - Western blotting
1 - DNA probes
2 - PCR
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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IICA / CIRAD-EMVT Questionnaire, June 1998
SIZE
(m)
TECHNIQUES USED
List them
LIST THE MAIN PATHOGENS / RESIDUES
(where applicable)
AVG NO ANALYSES / YR
(for each path./residue)
RESIDUE ANALYSIS
1 - Heavy metals
2 - Antibiotics
3 - Hormones
4 - Insecticides/acaricides
5 - Other
-
-
-
-
-
-
-
-
-
-
EXPERIMENTAL ANIMAL
FACILITIES
- Large animals
- Small animals
- Capacity (Species, No of animals)
- Degree of containment :
- Specialized units
- Capacity (Species, No of animals)
- Degree of containment :
- Specialized units
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IICA / CIRAD-EMVT Questionnaire, June 1998
III - HEAVY LABORATORY EQUIPMENT
(For each equipment describe number, type, use.)
Centrifuges - Low speed
- High speed
- Ultracentrifuge
Microscopes - Optical
- Inverted
- Fluorescence
- Other
Incubators - Regular
- Gas (CO
2
, N
2
, ...), anaerobic
Laminar flow cabinet
Thermoregulated baths (with agitation...)
Electrophoresis equipment
Blotting Protein / DNA equipment
Chromatography
Spectrophotometry (ELISA reader, other)
DNA hybridization
Thermocycler (PCR)
Special residue testing equipment
Other
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IICA / CIRAD-EMVT Questionnaire, June 1998
IV - SAFETY - HYGIENE
1/ Washing room, preparation of media, sterilization
Special room yes size (m) :
No if not, where
Preparation of microbiological media - Manual
- Automated
Sterilization - Autoclaving - Capacity (Vol / No Units) :
- Dry heat - Capacity (Vol / No Units) :
Storage capacity at +4C :
2/ Microbiological hazard
2-1 / Storage of parasites and microorganisms
Continuous passage of pathogens in culture
no
yes List pathogens:
Special storage room Yes size (m) :
No if not, where
Storage
-20C Capacity (Volume / number of units) :
- 80C Capacity (Volume / number of units) :
Liquid nitrogen Capacity (Volume / number of units) :
Freeze drying Capacity (Volume / number of units) :
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IICA / CIRAD-EMVT Questionnaire, June 1998
2-2 / Work with microorganisms
Safety cabinets no
yes Degree of containment
Laminar flow hoods - horizontal flow Number & class :
Location & use :
- vertical flow Number & class :
Location & use :
Special accreditation - Pathogens no
yes list them :
on process list them :
- Genetically Modified Organisms
no
yes list them :
on process list them :
2-3 / Disposal of infectious material
Inactivation by chemicals no
yes describe :
Heat inactivation no
yes - Autoclaving
- Incineration
Disposal process By the laboratory
By a specially licensed firm
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IICA / CIRAD-EMVT Questionnaire, June 1998
3/ Chemical hazard
3-1 / Chemical storage
Special storage room yes size (m)
No if not, where are they stored
3-2 / Work with chemicals
Products used - solvents list & explain :
- mutagens list & explain :
- radioelements list & explain :
- other toxics list & explain :
Chemical hoods no
yes Number :
Type :
Location & use :
Accreditations for radioelements no
yes list & explain :
3-3 / Chemical disposal
Disposal process By the laboratory Describe :
By a specially licensed firm
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IICA / CIRAD-EMVT Questionnaire, June 1998
V - STAFF
Number
(detailed)
Degree of
education
Specialization Which area
do they work
in ?
Supervisory staff
Support staff
Scientists
Technicians
On going training program no
yes In service describe
External describe
Participation in conferences & seminars no
yes List (past 2 years) :
Visits to other laboratories no
yes List & purpose (past 2 years) :
Membership in scientific associations no
yes List & purpose (past 2 years) :
Membership in regional / international associations (OIE...)
no
yes List & purpose (past 2 years) :
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IICA / CIRAD-EMVT Questionnaire, June 1998
VI - DOCUMENTATION - DATA STORAGE - COMMUNICATION
1 / Library & access to new information
Library in the laboratory no
yes Size (m) :
Journal subscriptions no
yes List the 5 most important :
Access to other libraries in the country (University...)
no
yes List :
Access to bibliographic data-bases (Medline, Current Contents...)
no
yes - Manual
- Computerized
- Internet access
Utilisation of external reference laboratories
no
yes - In the Caribbean
List & purpose :
- Outside the Caribbean
List & purpose :
VII - QUALITY CONTROL
Data storage - tracking of samples & degree of computerization (describe)
Internal quality control no
yes Describe :
External quality control no
yes Describe :
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IICA / CIRAD-EMVT Questionnaire, June 1998
VII - COMMENT ON ANY ASPECT OF YOUR LABORATORY THAT WOULD NOT APPEAR
IN THE QUESTIONNAIRE
VIII - LIST AND COMMENT ON THE MAIN CONSTRAINTS FACED IN YOUR LABORATORY
(Safety and disposal of hazardous material, training, equipment maintenance,
communication....etc)
IS YOUR LABORATORY WILLING TO PARTICIPATE IN A CARIBBEAN LABORATORY
NETWORK YES NO
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