Central Institute Research Goat

CIRG
Annual Report 2013-14

Annual Report
2013-14

CENTRAL INSTITUTE FOR RESEARCH ON GOATS
Makhdoom, Farah, Mathura 281122, U.P.

1

Publisher:
Dr. S.K.Agarwal
Director,
CIRG, Makhdoom
Editorial Board:
Chairman
Dr. S.K. Jindal
Members
Dr. P.K. Rout
Dr. S.D.Kharche
Dr. M.K.Tripathi
Dr. Vij ay Kumar
Dr. A.K.Mishra

Photography
Mr. Satish Chandra

CENTRAL INSTITUTE FOR RESEARCH ON GOATS
Makhdoom, Farah, Mathura 281122, U.P.

Telephone No. : 0565-2763380
Fax No. : 0565-2763246
E-Mail : [email protected]
Website : http:// cirg.res.in
Helpline :0565-2763320

Printed by :
Vij ay Printing Press, Mathura
D-27, Industrial Area Site 'A', Mathura
Tel. : 0565-2490354, 09412279156,
REFACE

Goat, the poor
mans cows,
fit in amicably
to achieve the the inter-
dependent obj ectives of
poverty alleviation,
availability of food, creation
of employment and growth
in rural income. The
livelihood security of an
incredibly large number of
farm families is linked to
livestock . Maj ority of small
and marginal farmers derive their livelihood
from goats. In order to make goat farming more
remunerative continued research efforts and
innovations for development of low cost
technologies, machines and tools are necessary.
The Central Institute for Research on Goats,
(CIRG) is moving forward to tackle these
problems by focused research, visionary
approach and road map on goat development for
nutritional security and prosperity of the
country. The Institute is focusing on emerging
issues like climate change, natural resource
degradation, feed and fodder availability,
appearance of new host-parasite complex,
concerns for health and bio-safety, restrictive
trade regimes, competitiveness of goat produce,
improvement in income of goat farmers, shift in
the consumption pattern towards processed food
and increased demand for food items of animal
origin especially goat.
The Institute has developed farmers friendly and
commercially viable technologies for goat
improvement. The Institute scientists have filed
18 patents, commercialized one technology and
recently developed technologies for diagnostics
of brucellosis & J.D. and vaccine against J.D.
which are under process of commercialization.
The Institute is maintaining elite herd of Barbari,
Jamunapari and Jakhrana goats and
Muzaffarnagri sheep for distribution of superior
females and males for the genetic improvement
of goats and sheep under field conditions. The
Institute during the year supplied 470 elite goats
and 65 sheep to different SAUs, State
Governments, NGOs and farmers for genetic
improvement of their
goats and sheep. The
mortality rate of goats
and sheep have been
below 5% during the
year which is mainly
due to effective health
care and management
of the livestock farms.
The scientists have
worked on 19 institute
funded and 17 out
funded research
proj ects. Whole genome sequencing of
Mycobacterium paratuberculosis, TLR expression
studies in brucellosis , characterization of Peste
des petits ruminants virus , in vivo development
of parthenogenetic embryos, identification and
formulation of low methane producing feed ,role
of azolla as protein source in goat feed,positive
genetic trend in production in Jamunapari,
Barbari and Jakhrana goats and Muzaffarnagari
sheep are some of the salient achievements of the
year. Agro-forestry is one of the important
sections of the Institute which produced and
supplied 9649 quintals of green fodder to
different livestock units, 251 quintals barley &
oat grains besides 7.40 quintals of cowpea and
guar seed. Scientists have published 122 research
papers in International and National Journals of
repute.
During the year, SFC and Vision 2050 documents
were finalized and approved by the Council.
These documents will serve as guiding force for
all our future programs. The All India
Coordinated Research Proj ect on Goat
Improvement (A.I.C.R.P.) with fourteen research
centres all over the country is running at this
institute. The Council has approved four new
centres under A.I.C.R.P. on goat improvements
in XII
th
Plan SFC. ie Changthangi goat unit at
SKUAST, Kashmir; Andaman goat unit,
Portblair; Uttarakhand goat unit, Pantnagar and
Himalyan goat unit, IVRI, Mukteshwar. Now
under AICRP, there are 18 units covering 13
breeds of goats in the country.
We have introduced designer training
programmes with prioritized content for
P
CIRG
specialized groups under sponsored training
programmes for the dissemination of
technologies of the Institute. We conducted four
National training programmes on scientific goat
production for the capacity building and human
resource development of goat farmers across the
country. There was a total of 16 trainings
organized during the year which included
trainings sponsored by DADF, Punj ab
Veterinary Council, Odisha Veterinary Council,
BAIF, Bankers Institute for Rural Development ,
Govt. of Bihar and Jharkhand. The Institute
activities were covered extensively by All India
Radio (AIR) and Doordarshan (DD) New Delhi.
Regular Clinical Camps were organized in
adopted villages for enhancing goat production.
We have proud distinction of offering widest
possible range of solutions to the problems of
goat farmers and enterpreneurs. The Institute
participated in nine exhibitions and Kisan Melas
at different place of the country to display its
various technologies for the benefit of the goat
farmers, professionals and other stakeholders
and awarded 1
st
, 2
nd
and 3
rd
prizes at DUVASU
,Mathura; NDRI, Karnal and IVRI, Izatnagar,
respectively.
The Institute is working in the area of
development of strong liaison amongst research
institutes for collaborative research and
developmental programmes in the area of goat
development and marching towards its goal in
this competitive environment by following the
path of ethics, values, dedication, research
excellence and enhanced social values.
The Institute scientists were awarded with Ram
Lal Agrawal Gold Medal , ISSAR fellowship ,
President, ISSAR; Vice-President of ISSGPU;
Member board of Management, NDRI, Karnal,
MAFSU, Nagpur; Member selection board
GADVASU, Ludhiana; RAJUVASU, Bikaner;
JNU, New Delhi; PDC, Meerut and ASRB, New
Delhi , IVRI, Izatnagar and NDRI, Karnal.
I express my sincere gratitude to Dr. S.
Ayyappan, Secretary DARE, and Director
General, ICAR, for his support for the
development of the Institute. I am thankful to Dr.
KML Pathak, DDG (Animal Sciences), Dr. B.S.
Prakash, ADG (AN&P), Dr. Gaya Prasad, ADG
(AH), Dr. Ravinder Singh Gandhi, ADG (AP&B),
and other scientists of SMD for their ever
encouraging support for the progress and overall
development of the institute. Our thanks are also
due to Chairman and members of RAC and IMC
of the Institute for their valuable guidance and
support. The editorial team needs appreciation
for their untiring efforts for compiling and
editing of the Annual Report. I hope the annual
report will be useful for scientists,
administrators, entrepreneurs and stakeholders
working in the field of goat production.

(S.K.Agarwal)
Director

Contents

Executive Summary .......................................................................................................1
CIRG: An Introduction....................................................................................................4
Organizational Setup .....................................................................................................7
Staff Posi tion......................................................................................................................8
Goat Genetics and Breeding Division ......................................................................9
Physiology, Reproduction and Shelter Management Divi sion ........................22
Nutrition, Feed Resources & Production Technology Divi sion ........................33
Goat Health Divi sion ....................................................................................................45
Extension Education and Soci o-Economics Section ..........................................66
AICRP on goat improvement.....................................................................................69
Exhibition/ Technology Di splay /Ki san Mela ........................................................74
Agriculture Farm and Agroforestry Section ...........................................................76
Technology Services ....................................................................................................78
Awards and Recognitions ..........................................................................................78
Publications ....................................................................................................................80
Human Resource Development .............................................................................100
Research Projects .......................................................................................................106
Distinguished Visi tors ..................................................................................................108
Personnel .......................................................................................................................109

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1

Executive Summary
ndia with 154 million goats is one of the
largest goats owning country in the world
and playing a significant role in livelihood
and nutritional security as well as providing
supplementary income to nearly 70 million
farmers of over 5,00,000 remote villages in the
country. Goat meat production in the country
has increased from 4.70 to 5.96 lakh tons during
the last decade (2002 to 2011) with an annual
growth rate of 2.4%. Similarly, goat milk
production in the country has also increased
from 36.4 to 45.9 lakh tons during the same
period with annual growth rates of 2.6 %. The
country stands first in goat milk production and
is the second largest in goat meat production in
the world by sharing 29% & 12% goat milk and
meat production, respectively. Goat meat
(Chevon) is most preferred and widely
consumed meat in the country. Since ancient
times goat milk has traditionally been known for
its medicinal properties and has recently gained
importance in human health due to its proximity
to human milk for easy digestibility and its all
round health promoting traits. The goat sector
contributes Rs. 22,138 crores to the countrys
livestock GDP through meat (Rs. 11,932 crores),
milk (Rs. 5,513 crores), skin (Rs. 800 crores) and
manures (Rs. 1,594 crores).
Genetic Improvement Programme
A positive genetic trend was recorded for milk
yield in Jamunapari goat population showing
significant improvement in milk yield over the
years during 90 and 140 days of lactation. Total
213 improved Jamunapari goats were distributed
during the year to goat breeders for the breed
improvement of their flocks. Barbari goat is
known for its wide adaptability and institute has
supplied 241 improved goats for genetic
improvement programme. The positive genetic
correlations among lactation traits indicates
lactation yield of 90 days a reliable trait for
constructing selection indices. There was
significant increase in milk production in
Jakhrana goats indicating the average daily milk
yield of 1.32 liter and average peak yield 1.95
liter. Body weights of Muzaffarnagri sheep at all
stages were significantly higher during year 2013
as compared to previous two years. The average
daily gain of Muzaffarnagari lambs during 0-3, 3-
6, 6-9, 9-12 and 3-12 months were 159.81, 106.78,
75.53, 54.63 and 77.18g under semi-intensive
feeding management. The breed specific
expression analysis of Hsp70.3 revealed that
Sirohi goats had higher expression (2-8 folds) as
compared to Jamunapari, Jakhrana and Barbari
goats. It has been observed that the heat stress
tolerant individuals had 3 to 10 folds higher
expression of Hsp than heat stress susceptible
individuals. Goat based integrated farming
livelihood models were developed for livelihood
security in disadvantaged districts of
Bundelkhand region.

Physiology, Reproduction and Shelter
Management Programme
Semen of superior bucks of Barbari, Jamunapari,
Sirohi and Jakhrana breed were cryopreserved
for AI and other research purposes. The post
thaw motility, live sperm count, acrosomal
integrity and hypo osmotic swelling positive
spermatozoa were significantly higher in 0.01%
of EDTA and 1mg % of vitamin C fortified group.
Parthenogenetic embryos were produced and
their in vivo development observed up to 23
rd

and 28
th
days in two recipients. Intra cytoplasmic
sperm inj ected in vitro matured oocytes were
developed up to 8-16 cell stage. Stem cells up to
third passage were produced from 8-16 cell and
morula stage embryos. The female fetal skin
fibroblast cell monolayer attained the same
percent of confluences 24 h earlier than to male
I
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fetal skin fibroblast cell monolayer. Embryonic
cell colonies were passaged up to third passage
on goat fetal fibroblast monolayer and stored in
liquid nitrogen. Hormonal profile of Jakhrana
goats at different reproductive stages was
established. The growth of kids was better in
slatted floor housing system as compared to
kutcha floor.
Nutrition, Feed Resources and Products
Technology Programme
Leucaena leucocephala foliage may be used as
sole green fodder on goat feeding. It is found that
mustard oil meal, and combination of urea and
guar korma can replace conventional protein
supplement linseed cake in lactating goats
feeding, with improved lactation performance.
Integrated nutrient management in nursery
seedlings was perfected. Mustard cake inclusion
reduced methane production by 21.09 %,
whereas the concentrate pellet containing guar
korma and urea produced 4.83 % less methane in
comparison to linseed cake pellets. It was found
that quality of feed especially the protein content
is responsible for the level of methane
production. Methane gas production
significantly got reduced with increasing
proportion of azolla in goat feed. Meat from
intact animal had significantly higher total
omega-3 as well as omega-6 fatty acids.
Goat Health Programme
Considerable progress has been made in
development of herbal anthelmintic
formulations for goats and four potential plants
were selected for prototype formulation against
haemonchosis. Complete genome sequence of
Mycobacterium avium subspecies
paratuberculosis Strain S-5 of goat origin has
been carried out. Johnes disease vaccine
technology has been transferred to Biovet under
PPP mode and the vaccine is currently under
clinical trial for commercialization. Factors such
as season, age etc. were found to have significant
effect on fecal egg count in the goats infected
with Haemonchus contortus while, factors such
as type of birth, birth weight and sex had little
effect on the count. Toll like Receptors genes such
as TLR-1, 2, 3 were sequenced in full length first
time and expression of TLR- 4 and TLR-9 was
found to be higher as compared to TLR-2
suggesting their strong role in innate response
against brucellosis. Expression of TLR following
exposure of PPRV to Vero cells was studied and
a peak in TLR 3 and TLR7 expression was
observed at 1 and 3 hr. post-infection,
respectively. A unique PPR virus (PPRV/
Nanakpur/2012) that cross reacts poorly with
monoclonal antibodies and hyper immune
serum (against vaccine strain) was isolated
suggesting this PPR virus may evade the vaccinal
immunity. E coli and Cryptosporidium were
found to be the main causative agents of the
neonatal diarrhea in the kids of Jakhrana,
Jamunapari and Barbari goats. Salmonella spp.
and Rotavirus were also found capable to cause
diarrhea in the kids. Procedures for diagnosis of
Cryptosporidium spp. infection in goats were
standardized, and prevalence of the infection
was found higher in 0-15 days old kids and
during the summer season as compared to 15-30
days old kids and winter season, respectively.
Sex or breed had no correlation with the
occurrence of cryptosporidiosis. Various viral
and bacterial pathogens such as Orf virus, goat
pox virus, PPRV, MAP strain S-5 (Indian Bison
Type), Pseudomonas aeruginosa, Klebsiella spp.,
Pasteurella multocida, Listeria monocytogenes,
Salmonella spp., Brucella melitensis and Shiga
toxin producing E. coli from goats were isolated,
identified, characterized and submitted to
VTCC, Hisar for accessions. Concentration of
non-esterified fatty acids (NEFA) in serum was
found to be a better indicator of negative energy
balance in peri-parturient goats. The method for
confirmatory diagnosis of pregnancy toxaemia in
morbid goats by estimation of beta- hydoxy
butyrate in vitreous humor of eye was developed
and standardized. Main causes of death in goats
diagnosed by post-mortem analysis were found
as enteritis (17.46%), pneumonia (17.03%),
septicemia (6.98%), haemonchosis (4.37%), &
pregnancy toxemia (3.05%), gastro-enteritis
(2.18%) and toxaemia (2.18%).
Extension Education and Socio-
Economics Programme
Four national trainings on Commercial Goat
Farming and seven sponsored training
programme on Scientific Goat farming were
organized during the year. In addition five other
trainings were organized for specialized groups
like veterinary Officers, proj ect officers, bankers
with tailor made content to suit the clients
requirements. Institute participated in 9
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3
exhibition/Kisan mela at different places and
won first prize in Kisan Mela at DUVASU,
Mathura, 2
nd
at NDRI, Karnal and 3
rd
prize at
IVRI, Izatnagar. One hundred and thirty three
(133) technical letters were received and replied.
Institute also entertained 11,694 visitors from
different parts of the country and received 1785
helpline calls and answered accordingly.
The All India Coordinated Research Proj ect on
Goat Improvement (A.I.C.R.P.) with fourteen
research centres all over the country is running at
this institute. The Council has approved four
new centres i.e. Changthangi goat unit at
SKUAST, Kashmir, Andaman goat unit, Port
Blair, Uttarakhand goat unit, Pantnagar and
Himalyan goat unit, IVRI, Mukteshwar under
A.I.C.R.P. on goat improvements in XIIth Plan.
Now under AICRP, there are 18 units covering
13 breeds of goats in the country.
The Institute scientists have been addressing
various problems in goat production through
nineteen Institutes funded and seventeen
extramural research proj ects. The revenue
generation during the year was 141 lakhs.
Institute scientists were awarded and recognized
by various organizations for their contributions.
Three postgraduate students from IVRI
completed thesis research work for M.V.Sc
degree. Three students from GLA University
Mathura are conducting research work for PhD
degree under guidance of institute scientist s.
Three graduate students from GLAU, Mathura
completed one month summer training and one
PhD student from SHIAT, Allahabad, UP was
given expert guidance on HPLC analysis of plant
extract samples. One batch of BVSC and AH
Students from College of Veterinary science and
AH, Mathura completed training under
internship programme. Students of different
academic colleges and veterinary colleges visited
the institute laboratory and livestock Units. The
institute recently developed technologies for
diagnostics of brucellosis & J.D. and vaccine
against J.D. which are under process of
commercialization.
The institute scientists published 122 research
papers in various national and international
j ournals, 36 popular articles, 68 research
abstracts, 38 lead/invited papers, 22
books/chapters/bulletins/manuals and 11 radio
talks/ and three TV programmes covered by the
DD National

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CIRG: An Introduction
onsidering the significance of goats in the
agrarian economy of India, The Indian
Council of Agricultural Research established a
National Goat Research Centre at Makhdoom,
Farah in Mathura district of Uttar Pradesh on
12th July, 1976. The centre got the status of a full
fledged Institute on 12th July, 1979 and named as
Central Institute for Research on Goats. The
Institute is located almost at equi distance from
two famous places Mathura (22 Km), the birth
place of Lord Krishna, and Agra (32 Km) the
abode of world famous Taj Mahal. Director is the
head of Institute and its apex body like IMC,
RAC and QRT guide its research and other
activities. Presently 38 Scientists, 72 technical and
36 administrative personnel share the
responsibility to achieve mandate of the institute,
which has 4 research divisions and one section
including well equipped Library, ARIS cell, PME
cell, Agricultural farm, IPR Cell, Livestock farm
and Health Section. The Co-coordinating unit of
All India Coordinated Research Proj ect is also
located at CIRG. The proj ect aims at improving
production performance of different breeds of
goats distributed in different regions of the
country under farm and field conditions. The
Institute is well connected with modern
information and communication facilities
comprising landline phones 0565-2763380,
2763323 and helpline 0565-2763320. The profile of
the Institute can be visited at www. cirg.res.in
Vision
To develop - the Goat- as a source of livelihood
and nutritional security for future prosperity of
India
MISSION
Improvement in productivity of goats through
research, extension and HRD support.
MANDATE
To undertake research, training and extension
education for improving milk, meat and fiber
production of goats and to develop processing
technology of goat products.
OBJECTIVES
To undertake basic and applied research in
all disciplines relating to goat production
and product technology.
To develop update and standardize area
specific package of practices on breeding,
feeding, management, prophylactic and
curative health cover for goats.
To impart National and International
trainings in specialized fields of goat
research and development.
To transfer technologies for improving
milk, meat, fiber production, value
addition of goat products and capacity
building.
To provide referral and consultancy
services on goat production and product
technologies.
Highlights of Achievements
The institute has developed farmers friendly
and commercially viable technologies for goat
improvement in the country. So far, 18 patents
have been filed; one green drug technology
(Alquit) for control of ecto-parasites has been
commercialized to M/S Natural Remedies Pvt.
Ltd, Bangaluru. Value added goat meat and milk
products, area specific mineral mixture,
diagnostics for brucellosis and JD are under
process of commercialization. The scientists of
the Institute have successfully produced kids
from embryo transfer and through IVF. A strain
of Mycobacterium avium subspecies
paratuberculosis genotype Indian Bison type
strain S 5 of goat origin has been transferred to
M/S Biovet (P) Ltd, Bengaluru for development
and commercialization of indigenous vaccine
against Johns Disease (J.D.). Recently, in
recognition of its meritorious scientific
achievements and technology innovation, the
Institute has been bestowed with the prestigious
ICARs Sardar Patel Outstanding Institute
Award-2010. Some of the maj or achievements are
as follows.
Multiplication and conservation of elite
germ plasm of Jamunapari, Barbari, Sirohi
and Jakhrana breed of goat for genetic
improvement of indigenous goats.
Improved reproductive performance
resulting in higher population growth in
Jamunapari (94.65%) and Barbari (183%)
goat flocks.
Positive genetic improvement trend in body
weight at birth, at 3, 6, 9, and 12 month of age
C
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in Jamunapari goats, (0.120.03, 0.590.12,
1.580.19, 2.660.28 and 2.140.36,
respectively) and at 9 month (0.9990.213 kg)
in Barbari goats.
Significant improvement in milk yield in
Jamunapari, Barbari and Jakhrana goats
compared to their base population
performance.
Successful freezing of semen of Jamunapari,
Barbari, Jakhrana and Sirohi breeds, and
production of kids through AI in goats.
Standardized Embryo Transfer and IVF
technology in goats and successful
production of kids through above
technologies.
Characterized heat stress tolerant genes i.e.
AP-2 binding site in the promoter region of
hsp70.1 gene, Melanocortin 1 receptor
(MC1R) gene, Tyrosinase (TYR) gene and
Signal transducer and activator of
transcription 5 A (STAT5 A) gene to facilitate
further studies on resilience of goat
production system under changing climate.
Established genetic origin of Indian goat
breeds and genetic variation in Myf, leptin,
Pit I, FecB, SCD gene and HSPgenes in
Indian goats.
Developed complete feed pellet for efficient
growth (80g/d) in finisher kids. Strategic
supplementation of concentrate mixture @
1.2 % of the body weight for better growth
and meat quality of Barbari goats.
Better dressing percentage and meat quality
by supplementation of area specific mineral
mixture under intensive goat rearing system.
Identified anti-methanogenic feed resources
for goat production system.
Developed higher bio-mass producing
fodder system (Guar+ Lobia + Sunhamp) for
goats under rain fed conditions and Morus
alba based cost-effective agro-forestry
system for sustainable goat husbandry in
semi-arid and rain fed areas
Developed package of practices and
dynamic health calendar for goat farmers.
Determined fatty acids and mineral status of
milk of different Indian goat breeds.
Standardized process for preparation of
herbal functional milk, whey drinks, goat
milk and meat based biscuits, and low fat
cheese.
Developed low cost-protein and mineral
enriched value added goat meat products
using fresh goat spleen and herb
supplemented functional goat meat and milk
products.
Created baseline data on commercial goat
farming.
Technologies Developed/
Commercialized
ALQUIT- Ectoparasiticidal Drug for animals
(commercialized)
BRUCHEK-Dot ELISA Kit for diagnosis of
Brucellosis
ELISA Kit for diagnosis of Johnes Disease
Inactivated Johnes disease vaccine
Intra vaginal pessaries for oestrus
synchronization.
Modern goat appliances to reduce feed and
water wastage
Area specific mineral mixture
Low cost complete feed pellet
Cost-effective milk replacers for kids
Goat meat Murukku: A crispy food product
Goat meat Nimkee: A snack food
Goat milk based moisturizer soap ( Aj as)
Impact of Research
Improved productivity and genetic potential
of indigenous goats through supply of
superior germ plasm from institute to State
Animal Husbandry Department, other
developmental agencies and goat farmers
Facilitated in establishment of several small
and large commercial goat farms in different
parts of the country through different
training for capacity building to Veterinary
professionals, farmers and other
entrepreneurs on scientific goat rearing and
entrepreneurship.
Improved body weights of Jamunapari
(45.67% ) , Barbari (31.96%) goats at 12 month
age and kidding rate (1.4 in Jamunapari and
1.48 Barbari)
Created semen bank for important goat
breeds and standardized technique for
successful AI in goat.
Development of goat health calendar leading
to over-all reduction in mortality.
Development of diagnostic kit for JD and
Brucellosis.
Commercialization of ectoparasitical drug-
Alquit.
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6
Development of suitable milk replacer for
pre weaning kids.
Developed complete pelleted feed, feed
blocks and designing of low cost pelleting
machine that is being adopted by
commercial goat farmers for intensive goat
rearing

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Organizational Setup

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Staff Position
Category No. of post sanctioned No. of post filled
Director 1 1
Scientific 50 39
Administrative Staff 33 36
Technical 72 59
Supporting 104 96
Temporary Status 98
Total 260 329
Financial Statement (2013-14)
Plan (Rs. lakh) Non Plan (Rs.lakh)
Allocation Expenditure Allocation Expenditure
A.Recurring
Establishment charges 0.00 0.00 1396.00 1392.40
Wages 0.00 0.00 378.00 323.33
OTA 0.00 0.00 1.00 0.62
TA 8.00 7.18 3.60 2.54
Other charges 154.00 153.65 172.43 159.74
HRD 3.00 2.93 3.00 2.52
Total 165.00 163.76 1954.03 1881.15
B. Non-recurring
Equipments 35.00 34.90 7.00 6.69
Information & technology 2.04 2.03 1.00 0.99
Furniture 2.10 2.08 0.00 0.00
Library books & Journals 7.13 7.13 0.00 2.49
Livestock 0.00 0.00 0.00 0.00
Work 112.50 112.50 0.00 0.00
Land Development 0.00 0.00 0.00 0.00
Others 1.23 1.23 0.00 0.00
Total 160.00 159.87 8.00 7.68
Grand Total (A+B) 325.00 323.63 1962.03 1888.83

Revenue Generation
Particulars Amount
(in lakh)
Sale of Farm Produce 26.20
Sale of Meat/Meat Products 4.16
Income from royalty/Sale of Publications and Advertisement 8.07
License Fee 8.07
Application fee from candidates 1.20
Interest earned on short term deposits 62.25
Income generated from Internal Resource Generation 8.22
Miscellaneous Receipts 23.68
Grand Total 141.85

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9
Goat Genetics and Breeding Division
Improvement and sire evaluation of Jamunapari goats for milk production
P.K.Rout , Gopal Dass, Mahesh Dige ,N. Shivsaranappa, H. A. Tewari and S. K.Singh
Population growth
The annual flock strength of Jamunapari goats
for the year 2013-2014 showed opening balance
of the flock was 739 and closing balance was 741.
The population growth of the flocks was 125.4%
during the year. The overall mortality of the flock
during the year 2013-14 was 4.76%.
Production performance
The mean of body weights of kids at birth, 3, 6, 9
and 12 months of age over the year were 3.41,
12.11, 15.10, 19.50 and 26.48kg, respectively.
Parity of dam had significant effect on kids body
weight up to 6 months of age. Male kids
maintained higher weights at all growth stages
over their counterpart. Kids born as single also
showed significantly higher weights than those
born as twins or triplets. The average daily
weight gain (ADG) of the kids under intensive
management was 73.89, 120.78, and 111.89 g/day,
respectively during 3-6, 6-9 and, 9-12 month age
group. Least squares means of part lactation milk
yield in 90 days and 140 days were 86.45 and
111.16 liters, respectively, during the year.
Season of kidding had highly significant (P<0.01)
influence on both the milk yields. Parity had
significant effect on milk yield over the years.
Reproduction Parameters
Reproductive performance of Jamunapari goats
in terms of breeding efficiency and kidding
percent on the basis of does tupped were 86.03
and 121.10%, respectively. The kidding rate was
1.46. During this year, a total of 254 does kidded
373 kids , out of which single, twin and triplet
born kids were 138, 226 and 9 respectively.
Supply of improved germplasm
During year , 213 improved animals were
supplied to various developmental agencies,
farmers and state governments, non-government
organizations and progressive breeders for
genetic improvement in the field conditions.
Genetic Parameter estimates of milk
production performance
Estimates of variance and co-variance
components were obtained using the ASREML
program (Gilmour et al., 2002), initially fitting the
univariate models. Likelihood ratio tests were
carried out to determine the most suitable model
for each trait in the univariate analyses.
Subsequently, bivariate analyses were carried
out using an animal model to estimate the
genetic, phenotypic and environmental
correlations. Milk production data of 2217
Jamunapari goat during the period 1995 to 2012
was analyzed. Summary statistics for milk
production traits are shown in table 1. The mean
90 days, 140 days and lactation milk yield of the
Jamunapari goat over the 17 years was 80.18
litres, 113.98 and 124.82 liter, respectively. The
average lactation yield was 124.82 liter with a
lactation length of 179.5 days.
Table 1 Milk yield and lactation length traits of Jamunapari goats
Mi l k yi eld 90 days MY140 days Lactation milk yi eld Lactation Length
Number of Records 2217 1788 2099 2099
Number of does 2217 1788 2099 2099
Number of years 17 17 17 17
Mean 80.18l i tre 113.98 l i re 124.82 l i tre 179.5days
SD 33.3 38.1 51.06 42.17
Standard error 0.708 0.90 1.11 0.92
CV 41.6 33.48 40.90 23.49
Range/ Maximum 21.8-168.0 46.8-233.6 33.0-273.7 70-277
CIRG

The log-likelihood obtained for each trait in four
different models was compared (Table 2). The
most appropriate model for milk yield at 90, 140
and lactation yield was animal and maternal
effect. The appropriate model for lactation
length was direct (animal + PE) effect. Parameter
estimates fitting the most appropriate model for
milk yield and lactation length was presented in
Table 2.The estimates of direct heritability for
MY90,MY140 LMY and LL 0.577, 0.765,0.781and
0.148, respectively. The maternal variance and
variance due to permanent environment were
very low for all the traits under study. The
maternal permanent environmental component
due to dam and litter contributed negligibly. The
heritability estimates across different traits with
small standard errors (varies from 0.015 to
0.036) resulted from the large size of the data and
the precise estimate showed the genetic
improvement for milk production for 90 days
and 140 days period will be successful by
selection.

Table 2: Model effect and genetic parameter of Jamunapari goats
MY90 MY140 LMY LL
Components anim maternal anim maternal anim maternal anim pe
var direct 55329.2 54825.8 98190.3 95471.2 149108 138727 267.162 217.488
var-maternal 723.148 3137.35 11358
Var-pe 88.1905
var-res 39473.2 39422.3 26223.3 26148.4 27505.6 27358.8 1181.9 1158.29
pheno 94802 94971 1.24E+05 1.25E+05 1.77E+05 1.77E+05 1.45E+03 1.46E+03
se 4404.4 4427.8 6629 6649.9 8959.8 9016.6 50.329 51.913
h2 0.5836 0.5773 0.7892 0.7653 0.8443 0.7818 0.1844 0.1486
se 0.0267 0.0367 0.0152 0.0351 0.0106 0.035 0.0312 0.033
mat2 0.0076 0.0251 0.064
se 0.0282 0.0329 0.0346
0.0602
0.025
Log L -3242.62 -3242.59 -10580.7 -10580.4 -2573.18 -2571.15 -8557.1 -8553.05

Genetic trends were estimated for each trait by
regression of EBV averages on year of birth,
weighted by the number of animals in each year.
A positive genetic trend was obtained for milk
yield in Jamunapari goat population and
showing significant improvement over the years
in milk yield during 90 and 140 days. (Fig1)

y = 8.2407x - 16498
R = 0.4048
E
B
V
Kidd year
Genetic trend of milk yield during 90
days in Jamunapari goats
y = 13.281x - 26404
R = 0.0705
E
B
V
Kidd year
Genetic trend during 140 days milk yield in
Jamunapari goats
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11
Genetic improvement of Barbari goats for meat and milk production
M.K. Singh, Mahesh Dige, S. K. Singh, Nitika Sharma
Flock statistics and dynamics
The annual flock strength of Barbari
goats for the year 2013-14 was 690. The
population growth was 147% and overall
mortality of the flock was 3.5%. During this year
241 goats were supplied for breed improvement
to farmers and various goat improvement and
development agencies
Growth production traits (body weights)
The data on body weight growth viz birth, 3, 6, 9,
and 12 month of ages recorded from 2009-10 to
2013-14 were analyzed for effect of year, season
of birth, sex of kid and type of kidding. Weight
of dam was included in the model as an
independent trait. Year, season, sexes of kid, type
of birth and parity have significantly affected
body weight at different ages. The overall least
squares means of body weight of kids at birth, 3,
6, 9, and 12 month of ages for the year 2013-14
were 1.680.02, 8.320.09, 12.590.17, 18.010.37
and 21.060.44 kg respectively (Table 1). Kids
born during autumn season have attained
significantly higher body weight at 6, 9 and 12
months of ages. Single born kids were
significantly heavier than those born as multiple.
Similarly males were heavier than their
counterparts right from birth to 12 months of
ages. The estimates of heritability ( h2 ) for body
weight of kids at birth, 3, 6, 9, and 12 month of
ages were 0.1890.043,0.2310.048, 0.2600.051,
0.4940.074 and 0.6550.088.
Lactation performance
The lactation performance data were analyzed
from 2009 to 2013 for non-genetic effects i.e. year,
season, type of kidding, parity and polynomial
regression of weight of dam at kidding using
mixed model least square techniques. Overall
mean for 90 days milk yield, 140 days milk, total
lactation yield and lactation length for the does
kidded in 2013-14 were 57.90, 79.78, 68.27 liters
and 131 days, respectively. Does kidded during
spring season produced significantly higher milk
production for 90, 140 days milk yield, lactation
yield, and lactation length than those which
kidded in autumn season. 90 days milk yield
increases up to 3rd lactation then remained
highest in 4th and 5th parity thereafter declined
with the advancement of parity order (Table-2).
The estimates of h2 for MY 90, LMY and LL were
0.4650.131, 0.4830.133, 0.4450.129 and
0.3090.115 respectively. The genetic correlations
among lactation traits were of high magnitude
and positive in nature indicates part lactation
yield of 90 days are reliable for constructing
selection indices.
Reproductive performance
Overall mean for age and weight first mating,
age and weight after first kidding, kidding
interval and gestation period, breeding efficiency
on the basis of does available, kidding % (tupped
goat), goat produced multiple birth, and litter
size are given in Table 3.
Selection Differential
The selection differential for 9 months body
weight was 5.47 kg and that of the dams 90 days
milk yield was 7.09 liters. The high selection
differential indicates the further scope of
improvement through selective breeding in these
goats.

Table 1: Least squares mean of body weight growth (kg) in Barbari goats
Factor Body Weight at

Year
Birth 3M 6M 9M 12M
2011 1.850.16
(577)
7.700.08
(555)
11.660.13
(529)
16.280.20
(489)
19.260.24
(489)
2012 1.770.02
(443)
7.270.09
(398)
10.460.15
(359)
15.000.23
(288)
18.600.27
(288)
2013 1.680.02
(316)
8.320.09
(308)
12.590.17
(221)
18.010.37
(80)
21.060.44
(80)
**P<0.01, *P<0.05
Values in parenthesis are number of observations
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Table 2: Lactation Performance of Barbari Goats
Year 90 days
milk (litre)
140 days milk
(litre)
Lactation yield
(litre)
Lactation length
(days)
2011 50.460.85
(333)
71.441.77
(120)
59.111.17
(334)
123.261.36
(334)
2012 52.180.90
(288)
78.081.88
(97)
61.131.21
(295)
125.641.41
(295)
2013 57.901.04
(191)
79.781.71
(113)
68.271.41
(194)
131.381.64
(194)
**P<0.01, *P<0.05

Table 3: Reproductive performance in Barbari goats over the years
S.No Traits 2011-12 2012-13 2013-14
1 Age at first mating (days) 348.23.4
( 114)
362.97.4
( 109)
354.76.4
( 97)
2 Weight at first mating (kg) 15.541.07
( 114)
14.443.2 (
109)
15.012.3
( 97)
3 Age at first kidding (days) 492.15.4 (113) 406.98.3 (
109)
422.35.2
( 102)
4 Weight at first kidding(kg) 17.232.5
(113)
16.233.3
(109)
16.012.3
(102)
5 First kidding interval(days) 226.031.1 (37) 219.076.2
(67)
221.047.2
(54)
6 Gestation period (days) 143.81.5 (246) 146.71.4
(307)
145.41.4
(204)
7 Breeding efficiency / fertility ( does
tupped)
91.1 92.5 90.7
8 Kidding percentage ( does tupped) 140.3 135.0 145.3
9 Kidding rate (liter size) 1.54 1.46 1.60

Genetic evaluation and improvement of jakhrana breed for milk and growth traits
Saket Bhusan, Gopal Dass, A. K. Mishra
Jakhrana goats are maintained at CIRG,
Makhdoom for genetic improvement of goats for
milk and meat production. Adult females were
selected on the basis of 90 days milk production
and of 9 month body weight. Kids were selected
for future bucks and does on the basis of 9
months body weight to increase body weight of
kids and 90 days milk production of their dam.
Out crossing breeding method in Jakhrana flock
was conducted. Kidding rate is also considered
for selection the does and bucks for breeding.
Selective and controlled breeding was practiced
in the flock. The does were bred during May-
June and October-November only because more
than 85 % does comes in heat in these two season
followed by kidding in the months of October -
November and March-April. After kidding, kid
birth weight, sex and birth status of each kids are
recorded then kids are weighted 15- days
interval from birth to weaning and thereafter at
monthly interval up to 12 months of age.
Weaning of kids is generally done at 3 months of
age.
Population Dynamics:
There were 88 kids, 30 adult males and 110 adult
females in the Jakhrana Unit Thirteen animals
were culled on production ground and 16
animals on health ground and 17 animals were
died. Thirteen breeding males and 3 breeding
does were supplied to the farmers and other
government and non-government agencies for
breed improvement.
Kidding rate
Total 108 kids were born from 74 kidding during
the year 2013-14. Out of 108 kids, 59 kids (54.63
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13
%) were male and 49 kids (45.37 %) were female.
Out of 74 kidding, 43 does (58.11 %) gave single
birth, 28 does (37.83 %) produced twins and 3
does (4.05 %) gave triplet births. Over all
multiple births were 34 (43.24 %). The kidding
rate of Jakhrana goats was 1.47. Gestation period,
kidding interval and dry period of Jakhrana
goats were 151.450.52, 290.42 0.46 and
143.320.48 days, respectively.
Production of breeding bucks for breed
improvement in the field and farm.
Male and female kids were selected on the basis
of their 9 month body weight and 90 days milk
yield of their mothers. Does were selected on the
basis of 90 days milk yield.
Growth performance of kids and milk
production of nucleus flock:
Males were selected on the basis of 9 month body
weight for selective breeding. Pooled average
body weight at birth, 3 and 6 months of Jakhrana
kids born in 2013-14 increased as compared to
kids born during 2012-13.
Body weight of male kids at birth, 3 and 6
months of Jakhrana kids born in 2013-14.
Body weight of female kids at birth, 3 and 6
months of Jakhrana kids born in 2013-14
increased 6.02 %, 44.38 % and 26.42 %,
respectively than kids born in 2012-13. Body
weight of single born kids at birth, 3 and 6
months of Jakhrana kids born in 2013-14 were
increased. Body weight of multiple born kids at
birth, 3 and 6 months of Jakhrana kids born in
2013-14 were increased 5.84 %, 40.27 % and 46.77
%, respectively than kids born in 2012-13. Results
indicated that selection of bucks at 9 month body
weight also significantly affects the 3 and 6
month body weight. Milk production of Jakhrana
goats increased effectively from 2009 to 2012 each
year due to genotypic effect of selection.
Females are selected on the basis of 90 days milk
production for selective breeding. Therefore,
milk yield of 30, 60, 90, 120 and 150 days of does
kidded in 2013-14 than does kidded in 2012-13.
Average lactation production was 183.8010.23
liter. Average per day milk production of
Jakhrana goats was 1.32 liter. Average peak yield
of the flock was 1.950.07 liter and average time
of peak yield in the flock was 18.177.37. Lowest
variation in lactation length, lactation
production, peak yield and time of peak yield
were found to be 56-196 days, 25.84-306.60 days,
1.4-2.8 litter and 7-49 days, respectively.

Table 1: Least Square means of body weight of Jakhrana kids.
2012-13 Birth wt. 3 m wt 6 m wt 9 m wt 12 m wt
Pooled 2.600.03 (119) 8.070.12 (100) 11.630.27 (69) 16.040.35 (63) 21.340.46 (54)
Male 2.680.05 (63) 8.29.0.18 (54) 11.500.39 (35) 16.730.59 (32) 21.290.65 (28)
Female 2.490.05 (54) 7.840.19 (43) 11.770.40 (32) 15.210.39 (29) 21.170.73 (24)
Single 2.680.07 (33) 7.780.23 (23) 11.490.52 (22) 16.420.66 (21) 21.630.84 (19)
Multiple birth 2.570.04 (84) 8.170.15 (75) 11.700.32 (46) 15.840.58 (41) 21.180.58 (34)
2013-14 Birth wt. 3 m wt 6 m wt 9 m wt 12 m wt
Pooled 2.760.03 (109) 11.690.14 (67) 14.400.27 (27) - -
Male 2.860.05 (59) 12.000.20 (36) 19.560.57 (7) - -
Female 2.640.06 (49) 11.320.20 (31) 14.880.65 (12) -
Single 2.820.06 (41) 12.060.22 (26) 15.372.42 (06) - -
Multiple birth 2.720.05 (67) 11.460.18 (41) 17.111.00 (13) - -

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14
Table 2: Means of Milk Production (liter) of Jakhrana goats
Year 30 d 60 d 90 d 120 d 150 d
2012-13 45.911.18
(77)
83.321.99
(74)
113.013.20
(70)
146.264.28
(49)
165.1411.22
(29)
2013-14 51.169.25
(71)
97.233.80
(48)
141.285.12
(39)
178.226.38
(35)
209.2711.74
(16)

Genetic evaluation and improvement in muzaffarnagari sheep for body weight.
Gopal Dass, Souvik Paul and S.D. Kharche
Muzaffarnagari, the heaviest mutton producing
sheep breed of the country, is mainly distributed
in Muzaffarnagar and its adj oining districts of
Western Uttar Pradesh viz. Meerut,
Bulandshahar, Saharanpur and Bij nor. The breed
is usually reared for mutton production. The
institute has been maintaining a pure bred flock
of Muzaffarnagari sheep under a Network
Proj ect on Sheep improvement since 1976.
Presently the efforts are being made to improve
the breed for higher mutton production through
selective breeding.
Management of flocks
Flocks were maintained under semi-intensive
system of feeding management with 6-7 hours
grazing supplemented with 100-500 gm
concentrate in various stage and age group of the
animals. Dry and green fodder was also offered
as per the requirement. Controlled breeding was
practiced to improve the managemental
efficiency. Ewes were bred during May-June and
October-November followed by lambing in the
months of OctoberNovember and March-April,
respectively.
The opening balance of sheep was 550 which
comprised of 185 males and 365 females and
closing balance of 590 sheep had a stock of 197
males and 393 females. During this year a total of
219 lambs born and overall mortality was
recorded 3.64%.
Production performance
The overall least-squares means of body weights
of lambs at birth, 3, 6, 9 and 12 month age were
3.780.04, 18.180.27, 26.340.53, 30.780.66 and
35.490.75 kg, respectively. Sex and year of
lambing had highly significant (P<0.01) influence
on all body weights except non-significant effect
of sex and year of lambing on birth weight. Male
lambs gained higher weights as compared to
female lambs at all growth
stages. Body weights at all stages were
significantly higher in year 2013 as compared to
previous two years. The average daily gain of
Muzaffarnagari lambs during 0-3, 3-6, 6-9, 9-12
and 3-12 months were 159.812.76, 106.783.41,
75.533.29, 54.633.73 and 77.182.51g under
semi-intensive feeding management. The
average adult body weights of males and females
were respectively 56.5 and 41.1 kg.

The overall least squares means for lambs 1st and
2nd six monthly and adult annual clips were
calculated to be 478.2512.45, 492.8313.84 and
1119.3821.51g, respectively. The males
produced significantly higher greasy fleece yield
than females in all the clips which might be due
to larger surface area for wool growth in males as
compared to females.
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15
Growth performance of Muzaffarnagari lambs (kg).

Particulars Birth Wt.

3M Wt.

6M Wt.

9M Wt.

12M Wt.
Overall mean 3.750.02 (717) 16.840.15
(686)
24.250.25
(513)
29.070.28
(456)
33.410.33
(430)
Sex NS ** ** ** **
Male 3.790.03 (386) 17.450.20
(370)
26.240.32
(268)
31.820.36
(241)
36.890.42
(223)
Female 3.710.04 (331) 16.240.22
(316)
22.250.34
(245)
26.310.39
(215)
29.940.45
(207)
Year NS ** ** ** **
2011 3.720.05 (278) 17.020.24
(274)
23.950.32
(257)
28.600.33
(250)
32.460.38
(235)
2012 3.750.04 (211) 15.330.28
(196)
22.460.40
(165)
27.820.43
(144)
32.300.50
(134)
2013 3.780.04 (228) 18.180.27
(216)
26.340.53
(91)
30.780.66
(62)
35.490.75
(61)

Reproduction performance
The twinning rate in Muzaffarnagari sheep is
comparatively low due to large body size. But
due to the intensive breeding of those rams and
ewes responsible for producing twins and
triplets, the twinning rate improved
tremendously. The annual tupping, lambing on
available basis and lambing on bred basis were
93.4, 76.9 and 83.2%. These reproductive
parameters in season first and second were
respectively 95.4, 75.6, 80.5% and 95.4, 75.6,
80.5%. During this year, the twinning rate
recorded to be 9.1, 26.7 and 14.9% respectively in
first & second season and annual. The twinning
rate was found significantly higher than
previous years. The overall replacement rate was
calculated as 24.5%.
Distribution of elite germplasm
A total of 65 elite animals (51 rams and 14 ewes)
were supplied to various developmental
agencies, Research organizations, Non-
Government organizations and progressive
farmers for genetic improvement of their flocks
under field conditions.

NAIP Project: Goat husbandry based integrated approach for livelihood security in
disadvantaged districts of bundelkhand region
M.K.Singh, A K Dixit, A.K. Roy
Eight villages were selected from each of two
disadvantaged districts namely Hamirpur and
Mahoba of Bundelkhand. The following
programmes were implemented in an integrated
manner:
Goat based improvement and livelihood
programmes
Poultry based livelihood programme
Bovine (cow and buffalo) health and
production improvement
Fodder resource development
Crop improvement
Eco-friendly integrated insect-pest control,
Soil and water conservation measures
Capacity building of beneficiaries.
A total of 909 elite goats were provided to 364
resource poor people for strengthening their
income and nutrition. The milk yield from these
goats ranged from 78 to 164 liters with an average
106 liters in a lactation period of 125 days. It was
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16
115% higher over baseline values (49 liter). The
bodyweight of kids at 9 months of age born from
these pure bred and elite goats varied from 16 to
23 kg with an average of 18.2 kg. Multiple births
from these goats were also higher (15-22%) over
local non-descript goats. The price of yearlings
from these goats was 30-50% higher over non-
descript goats.
Fifty two superior breeding bucks were provided
for the up-gradation of villagers low yielding
and non-descript goats. Progeny born from these
bucks were superior in body weight and fetch
better price in the market. Simultaneously, scrub
bucks were disposed off and a kids nursery farm
from high milk yielding Bundelkhandi goats
was established with the support of a local NGO
to meet out the requirement of breeding bucks.
Bundelkhandi breed of goat was phenotypically
characterized.

Distribution of pure-bred and high potential goats and buck

Activities/Innovations implemented in adopted villages

Under promotion of package of goat improved
management practices, 45 low cost goat shelters
were constructed, 80 goat feeders, 150 water
devices 386 kg mineral mixture and 580 quintal
concentrate feed provided to beneficiaries. A
total of 11421 goats were vaccinated and 10313
goats were dewormed. These measures reduced
mortality from 26.4% to 8.5 %. Increase in the
survival of goat increased the net annual income
of Rs 3500 from a unit of five goats. Similarly,
feeding of concentrate increased the body weight
of kids in flock from 13.5 kg (before concentrate
feeding) to 17.6 kg at 9 month of age. The
concentrate ration provided to the goats helped
in prolonged lactation period by 20-30% and
shortened kidding interval, morbidity and
mortality. Supplementation of concentrate ration
increased milk yield of goat by 39% . Three
hundred eighty six kg mineral mixture was
distributed to 322 goat keepers to strengthen
immunity, health, fertility and productivity of
goats. Construction of low cost goat shelters
CIRG

17
decreased the morbidity by more than 35%. Low
cost shelter is getting popular among goat
farmers. Organized marketing for the sale of
grown-up kids was introduced with Sir Tata
Trust through a NGO at Rs 150 kg live body
weight basis to increase competition. Goat
keepers have also made insurance of their costly
goats and breeding bucks. One cooperative goat
farm was established in Hamirpur district with
about 350 goats by adding resources of 22
farmers belonging to 5 villages. 12 medium scale
commercial farms were established in the region.
The overall impact of goat based interventions
resulted in increase in survivability (21%), milk
yield (70%), body weight of kids at 12 month
(44%) and multiple births by 105%. Increased
productivity and survivability resulted in
increase in income by 250% on one adult goat ie
net gross income with five adult goats increased
from Rs 6758 to Rs 23705. Moreover, a unit of 2-5
goats with an average of 2.7 goats were provided
to 364 poor people (land less marginal and small
farmers) who were earning Rs 12000 to Rs 21000
with an average of Rs 14223/year from a unit of
3 adult goats. A unit of goat with five adult
females provides employment for 182 man
days/year.
Large ruminants are available with 76%
household. Fodder intervention was mainly
utilized by farmers keeping buffaloes and cows.
Increased fodder availability to beneficiaries
facilitated enhanced milk production by 34%.
Supply of five hundred kg mineral mixture,
deworming for endo-parasites and strategic
supplementary feeding have also supported
survivability and productivity of cows and
buffaloes. These measures have increased the
income over baseline from Rs 21175 to Rs 31325
per year with a unit of 3 bovine.
Supply of 8899 chicks to 400 beneficiaries has
increased monthly income of beneficiaries from
3.25 to 13.75% with an average increase 8.11% (Rs
11,612/annum) under backyard poultry
intervention. However, adoption of backyard
poultry was low due to high mortality of chicks
mainly due to predation and theft. Backyard
poultry has great potential as additional source
of income in the region.
Intervention of seeds of improved varieties of
pulses ( black gram, pigeon pea) and oilseed
(sesame) crops was provided which increased
the production yield of these crops by 56 to 136%
with an average income of Rs 5360/household/
season (crop).
New fodder crops (oat, sorghum, multicut
baj ara, guar, napier grass, napier-baj ara hybrid),
seeds of improved varieties, horti-silvipasture,
range grasses and perennial grasses wer e
introduced to proj ect beneficiaries for field
demonstrations under fodder resources
development. Seven hundred twenty five (725 )
demonstrations were conducted in different
seasons in 394 farmers field with an average area
of 0.03 ha/farmer. Farmers produced 350-400 q/h
fodder in Zaid, 400-450 q/h fodder in Kharif and
>600 q/h in rabi season. Increased fodder yield
with high yielding varieties were higher by 40 to
60% over local varieties. Fodder from these crops
was also sold as cash crop. Increase in availability
of green fodder had increased the milk yield of
buffaloes by 22-65% and, 12-30% in case of cows
and goat. The fodder availability was increased
by 10-15 kg over base line value of 5 kg
fodder/buffalo/day and high yielding cows.
Average milk yield of buffaloes, cows and goats
were increased by 44, 33 and 16% respectively.
Cultivated green fodder was predominately fed
to buffaloes and high yielding cows. Sorghum
(Zaid), Cowpea+Sorghum/baj ara (Kharif),
Berssem+oat (Rabi) were identified as suitable
fodder crops for this region. In low fertile land
seeds and rooted slips of guinea grasses, napier -
baj ra hybrid and Stylosanthes hamata,
Cenchrusciliaris were provided to 99 farmers of
16 villages in 0.031 ha/farmer area. Yield from
range grasses varied from 200 to 650 q/ha with an
overall average of 410 q/ha. Guinea grass,
Cenchrusciliaris,and Stylosanthes hamata were
found suitable grasses for this region. Adoption
of range grasses by the farmers was moderate
due to lack of protection from stray grazing.
Units of horti-silvipasture were established in 46
hectare land available with 82 farmers.
Plants/seedlings of fruit cum fodder trees of
papya, aonla, guava, citrus, j ack fruit, karonda,
peepal, mango, pakar, subabul, bair and
bahuhinia were provided to farmers. Few units
have started producing fodder @ 150-180
q/ha/year. Survivability of plants was low due to
inadequate irrigation and stray grazing. Papaya
+guinea grass and Cenchrus ciliaris found
appropriate combination for horti-silvipasture.
CIRG

18
Home-made insecticides such as neem seed
kernel extract, trichoderma, bio-control agents
(Beauveria bassiana), pheromone traps and bird
perches were introduced as interventions. These
interventions increased the crop yield by 9-31%.
Its effect on vegetable crop was more
pronounced followed by pulses. It also yielded
an additional income of Rs 1500 to 5000 per acre
with an average of Rs 3150/HH/Year.
More than 266 farmers were trained and
motivated for harvesting of rain water through
different types of trench and bund. About 30-35%
run-off of rain water saved through these bunds
helped increase in the crop yield by 10-15% i.e.
savings of Rs 750-7500 per hectare with an
average of Rs 3270/HH/year.
People were trained for preparation of paneer,
j ams, j elly, sauce, spices, muraba and chatni, of
various fruits and vegetables and also for tie and
dye of cloths through 59 demonstrations under
value addition of livestock and crop products
and their marketing. More than 167 field days,
kisan gosthi, farmers day, health camps, training
and front line demonstrations were conducted
for capacity building and skill development.
More than 2760 rural households were enrolled
under above group of capacity building. These
trainings equipped the villagers with better
confidence and knowledge levels.
Livelihood models for different categories
(resources) farmers were suggested based on
implemented interventions. Six thousand seven
hundred fifty five farmers were covered under
different proj ect interventions. Goat and poultry
were providing additional income of Rs
25835/year along with 250 man-days
employment per year. The additional income
from a unit of goat (5), poultry (25 chicks), crop
(improved seed sown in 0.4 ha), fodder (0.035
ha), insect-pest control and soil and water
conservation measures were Rs 18348, 11612,
5360, 11100, 7875 and 3270 per year. All above
interventions together created employment of
343 man-days per household per year.

Outputs and Outcomes
S.No Activity Baseline value Target Achievement
1 Body weight at 12 month (kg) 16.6 18.0 23.8
2 Total milk yield/lactation (litre) 49.0 80 83.5
3 Lactation period (days) 85.0 110 129.0
4 Goat mortality (%) 25.5 15 8.5
5 Goat abortion (%) 25 10 9
6 Bovine milk yield (liter/d) 3.5 4.5 4.7
7 Bovine mortality (%) 10.0 5.0 4.5
8 Buffalo conception (%) 65 80 80
9 Bovine abortion (%) 35 15 12
10 Backyard poultry farm in villages 12 150 200
11 Egg production/chick/year 70 120 150
12 Use of eco-friendly pesticide by farmers - 150 140
13 Fodder availability (q/ha/y) 7.0 15.0 21.0
14 Area under fodder crop (ha) 0.023 0.03 0.037
15 Pulse crop yield q/ha(rain-fed) 4.5 7.5 8.0
16 Oilseed crop yield q/ha (rain-fed) 4.0 7.0 7.5
17 Monthly income of landless, marginal and
small farmer (Rs)
250, 480, 650 800, 1000,
1500
1600, 2550,
3800

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19

Table :Recommended Goat based Integrated Livelihood Models
Model

Unit

Net income (Rs)

Suitability

House-holds
Covered

Goat+
Poultry
15 adul t F+
25 Chi cks
Rs 82727
(71115+11612)
Landl ess
Margi nal
64

Goat+
Cow+
Poultry+
Crops (Rain-fed)
10 adul t F+
2 cows +
50 chi cks +
1 ha.
Rs 100634
(47410+22000+
23224+8000)
Landl ess
Margi nal
Smal l
142
Goat+
Buffaloes+
Cows+
Crop (semi-irri)
5 adul t F +
2 buffal oes+
2 cows +
2 ha.
Rs 109705
(23705+34000+
22000+30000
Margi nal
Smal l
Medi um l arge

80

Goat
Buffaloes
Cows
Crop (semi-irri)
10 adul t F
2 buffal oes
2 cows
2 ha
Rs 119000
(47410+32000+
22000+30000)

Semi -medi um
Medi um
Large

56

NAIP: Bioprospecting of genes and allele mining for abiotic stress tolerance
P.K. Rout, N. Ramachandran and S.K. Jindal
Genotyping by HRM analysis
HSP70.3 gene
The genotyping of HSP70.3 gene was carried out
by HRM analysis for the region 1 to 304 bp and
2200 to 2700bp . HRM analysis showed 5
different genotype; the samples were confirmed
by sequencing analysis. We obtained 100 percent
concordance between HRM genotyping and
sequence analysis. Two SNP was observed in this
region of the gene which can be used for
genotyping the goats in response to heat stress.

Fig : Normalised and temperature shifted difference plot of HSP70.3 gene in four different breeds
indicating different genotypes in analysed samples
Nox gene
NADPH oxidase (Nox) proteins are membrane-
associated, multiunit enzymes that catalyze the
reduction of oxygen using NADPH as an
electron donor. Nox proteins produce
superoxide ( O2
) via a single electron reduction.

Nox proteins have been shown to regulate many
fundamental physiological processes, including
cell growth, differentiation, apoptosis, and
cytoskeletal remodeling. In addition, they have
more specialized functions, such as host defense
(Nox2Nox gene in goat was analysed by HRM
and obtained 4 genotypes in this region and was
also confirmed by sequencing.

CIRG

20
Fig: HRM genotypes as differentiated by different colour for Nox gene in four different breeds

Fig : Normalised and temperature shifted difference plot of Nox gene in indicating different genotypes
in the analysed samples
Gene expression analysis in different tissues
in response to heat stress
The individuals belonging to four breeds were
phenotyped based on respiration rate and heart
rate. The contrasting genotypes were identified
and individuals slaughtered during peak heat
period and high THI .The differential gene
expression was analysed for Hsp70 gene, HSP90,
Leptin in goat.
Expression analysis of Hsp70.3 was carried out in
different organs in four different goat breeds by
Relative quantification RT-PCR. GAPDH gene
expression was used as internal control for all the
replicates. The results indicated that the Hsp70.3
gene expression was 5 Folds higher in spleen and
kidney than heart and brain. (Fig)

Fig: m-RNA Expression of Hsp70.3 gene in liver and spleen of goats by relative quantification RT-
PCR. GAPDH expression was used as internal control for all analysis.
The breed specific expression analysis of Hsp70.3
revealed that Sirohi goats had higher expression
as compared to three breeds. Similarly,
Jamunapari goats had 2 fold higher expression
than Jakhrana and Barbari goats (Fig).

Fig: Relative normalized expression of Hsp70.3 by relative quantification RT-PCR in different
goat breeds (Mean SE)
Based on the cardinal physiological responses,
the individuals are defined as heat stress tolerant
and heat stress susceptible phenotypes. The
Hsp70.3 gene expression pattern with in these
two phenotypes in four different breeds showed
that the heat stress tolerant goats had higher
expression (varied from 2 to 24 folds) as
compared to heat stress susceptible phenotype.
(Fig)
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21

Fig: Relative normalized expression pattern of Hsp70 gene in different phenotypes in four
different goat breeds by relative quantification RT-PCR. GAPDH expression was used as internal
control for all analysis.
The expression analysis carried out in tissue
extracts of different organs and expression
pattern was analysed in liver and spleen on a
within heat stress susceptible and heat stress
resistant phenotypes. It was observed that the
heat stress resistant individuals had 3 to 10 folds
higher expression than heat stress susceptible
individuals.
Expression of CRP in different tissues
The expression of C-reactive protein in plasma of
different breed on a within heat stress tolerant
and heat stress suceptible individuals are also
analysed.

1
2
5
1
.
2
8
3
1
2
2
6
.
2
9
6
1
2
4
8
.
4
2
6
1
2
4
5
.
5
2
7
1
2
9
9
.
5
9
9
1
3
2
8
.
1
7
5
1
2
7
3
.
7
9
6
1
3
1
1
.
9
6
8
1150
1200
1250
1300
1350
1400
Barbari Jamunapari Jamunapari Kids Overall
Expression of Bovine CRP protein in diffrerent breeds in
response to heat stress
susceptible Resistance
CIRG

22
Physiology, Reproduction and Shelter Management Division
Improvement of post-thaw quality and fertility of frozen semen of different breeds of
goats using various additives
Satish Kumar, S.K. Jindal, A.K. Goel, S.D. Kharche, Ravi Ranjan and Chetna Gangwar
Proj ect is aimed to achieve better post thaw
motility and fertility by using various semen
additives and improve freezing protocols using
automatic freezing machines. It also included
strengthening of existing semen bank by storing
sufficient number of frozen semen of elite goat
breeds and widespread use of AI for augmenting
productivity of indigenous goat breeds.
Semen freezing studies
Collection and evaluation of seminal parameter
from different breeds of bucks for breeding/AI
purpose from experimental shed of PR&SM
Division were done. Bucks of different breeds
were screened for seminal parameters and only
those bucks donating good quality semen, were
used for semen cryopreservation and subsequent
AI. Semen of superior bucks of Barbari,
Jamunapari, Sirohi and Jhakhrana breeds were
cryopreserved for AI or other research purposes.
Vitamin C and EDTA were added in diluents as
an additive to find its effect on the freezing
quality, post thaw seminal characteristics and its
fertility.
Use of chelating agent as semen additive and
its effect on post thaw quality
Thirty five semen ej aculates collected from adult
Sirohi bucks (2-4 years old) maintained under
semi intensive management system of Institute
were utilized for the present study. The
ej aculates were collected twice in a week using
artificial vagina and were extended in such a way
so as to maintain sperm concentration to the tune
of approximately 100 million per dose (0.25 ml)
with Tris- Citric acid- Fructose (TCF) diluent;
having 10% (v/v) egg yolk and 6% (v/v) glycerol.
Four concentrations of EDTA (Ethylene Diamine
Tetra Acetic Acid - EDTA: 0%-as control, 0.01%,
0.05% and 0.1%) were used to find out its effect
on various semen freezability parameters of
buck semen. The diluted and processed semen
was frozen by conventional method of freezing
using Liquid nitrogen vapor. Filling and sealing
of straws were done at 5
0
C in cold handing
cabinet and after 4 h of equilibration period
straws were frozen in liquid nitrogen vapor for
10 minutes above 2 cm of liquid nitrogen level
and finally plunged in to liquid nitrogen. Post
thaw motility, live sperm count, sperm
abnormalities, acrosomal integrity and hypo
osmotic swelling test were performed. Analysis
of data using SPSS 16 revealed that post thaw
motility, live sperm count, sperm abnormalities,
acrosomal integrity and sperm responsive to
hypo osmotic solution differed significantly
(P<0.05) in different concentrations of EDTA. The
post thaw motility, live sperm count, acrosomal
integrity and hypo osmotic swelling positive
spermatozoa were significantly highest in 0.01%
of EDTA than with other concentrations used. It
could be inferred from these findings that 0.01%
EDTA can be used as an additive in semen
dilutor during routine freezing for better post
thaw recovery of buck semen.

Table 1. Effect of different concentration of EDTA in the dilutor goat semen dilutor on post thaw quality
of Sirohi buck semen
Concentration
of EDTA (%)
Progressive
motility (%)
Live (%) Abnormality
(%)
Acrosome
intact (%)
HOS (%)
0.0 (control) 36.140.41
c
55.141.00
c
2.730.10
b
70.671.01
b
49.261.04
c

0.01 44.860.48
a
63.130.95
a
2.560.09
b
75.000.89
a
59.741.08
a

0.05 42.790.60
b
58.660.92
b
2.760.09
b
71.350.86
b
55.040.82
b

0.1 29.280.92
d
44.761.14
d
3.930.77
a
66.711.03
c
38.111.08
d

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23
Effect of vitamin C as semen additive in the dilutor on post thaw quality
Effect of different concentration of vitamin C (L-Ascorbic acid) in goat semen dilutor on post thaw
quality of Sirohi buck semen
Concentration
(Milligram %)
Motility Live Dead Abnormal Acrosome HOS

0.0 (control) 41.041.08
c
56.921.49
c
43.081.49
a
3.580.61
a
76.671.43
b
41.711.37
b

0.8 47.291.16
b
61.871.43
b
37.291.75
b
3.120.58
a
78.671.36
b
46.581.41
b

1.0 53.541.29
a
68.211.37
a
32.621.32
c
2.830.59
a
83.621.15
a
53.921.93
a

1.2 44.171.89
bc
59.961.71
bc
40.461.68
ab
3.420.60
a
76.751.76
b
44.252.24
b

Pre freeze 84.051.23 89.710.94 10.760.92 2.860.64 91.570.91 75.431.03
Twenty four semen ej aculates collected from
adult Barbari bucks (2-4 years old) maintained
under semi intensive management system were
utilized for the present study. Ascorbic acid (L-
Ascorbic acid) in four different concentrations:
0%- as control, 0.8%, 1% and 1.2% milligram %)
were supplemented in the dilutor and the diluted
semen was frozen using conventional method of
vapor freezing. The ej aculates collected twice in
a week using artificial vagina were extended to
maintain sperms concentration of approximately
100 million per dose (0.25 ml) with Tris- Citric
acid- Fructose (TCF) diluent containing 10% (v/v)
egg yolk and 6% (v/v) glycerol. Filling and
sealing of straws were done at 5
0
C in cold
handing cabinet after 4 h of equilibration period
then straws were vapor frozen for 10 minutes
above 2 cm of liquid nitrogen and finally
plunged in to liquid nitrogen. Post thaw motility,
live sperm count, sperm abnormalities,
acrosomal integrity and hypo osmotic swelling
test were performed to find out its effect on
various seminal parameters. Analysis of data
using SPSS 16 revealed that post thaw motility,
live sperm count, acrosomal integrity and per
cent sperm responsive to hypo osmotic swelling
solution differed significantly (P<0.05) with
different concentrations of vitamin C in the
dilutor. The post thaw motility, live sperm count,
acrosomal integrity and hypo osmotic swelling
positive spermatozoa were significantly higher
in 1miligram % of vitamin C used in the present
study. It is conceivable at this st age that 1
milligram % of vitamin C can be used as an
additive in semen dilutor in routine freezing for
better post thaw recovery of buck semen.
Semen collection, and AI
Collection, Evaluation and Freezing of Semen
During the period under report, a tot al of 4010
semen does of different breeds of goat
(Jamunapari, Barbari, Jakharana and Sirohi )
were prepared and frozen out of the total 4010
semen does, 1108 semen doses /straws (sirohi
206, Barbari 1108) were used under different
experiment under the proj ect and demonstration.
Artificial Insemination with frozen semen
In two maj or breeding seasons (May June ; 44
and October :78) 122 goats of different breeds
(Jamunapari, Barbari, Jakharana and Sirohi)
were inseminated with frozen semen. Out of the
total insemination carried out in goats, 22 Barbari
exhibited short cycles after insemination (range
5-8 days) and 20 other goats (Jamunapari : 08,
Barbari :04, and sirohi : 08) shown the apparent
symptoms of low grade uterine infection, which
was evident during subsequent estrus period at
the time of insemination.
In remaining 80 goats inseminated with frozen
semen, 14 conceived and delivered a total of 19
kids.

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24
Hormone profile during different reproductive stages in goats
A. K. Goel, S. K. Jindal, Satish Kumar, S.D. Kharche and Ravi Ranjan
Gonadal hormones play a crucial role in
reproduction and production in goats. Meagre
information on hormonal profile in tropical goats
is available before and after puberty and during
peri- parturient period. Currently scanty
information is available regarding regulatory
mechanisms controlling post-partum oestrus
period in goats with non-seasonal reproductive
activity. Suckling has a negative effect on re-
establishment of post- partum ovarian activity.
Information on basal concentration of gonadal
hormones during different physiological stages
of tropical goats is lacking. The study shall be
useful in understanding the role of various
hormones playing role in the normal
reproductive process of goats. The outcome of
the proj ect shall be used to imply its influence in
improving the reproductive efficiency for higher
productivity from the goats.
Jakhrana goats of both sexes were selected and
grouped according to their
physiological/reproductive stages. Blood
samples (4 ml each) from 12 Jakhrana goats (6
each for male and female) at 10-12 months (Pre
pubertal) 13-14 months (Pubertal) and 15-16
months (Sexually mature) were collected at 15
days interval and serum samples after separation
were stored at -20
0
C till assayed for hormone
concentration. Blood samples from Jakhrana
pregnant does were collected at 30, 60, 90, 120
days and serum samples were stored and
assayed in similar fashion as above.
Hormone Assay (Testosterone and
Progesterone)
In male goats, for testosterone assay, 72 samples
(24 from each group in duplicate),in female goats
for progesterone assay, 72 samples (24 from each
group in duplicate) and 24 samples(in duplicate)
from pregnant does were processed by using
commercially available, Lab Serv (Fisher
Scientific, India Private Ltd.) ELISA Kits. Results
are summarized in following graphs
Testosterone concentration of the male goat at
different months approaching sexual maturity
Progesterone concentration of the female goat at
different months approaching sexual maturity

Progesterone concentration of female goat at
different months of pregnancy
NFBSFARA Project : Development of parthenogenetic goat from embryonic stem cells
S.D. Kharche, Ravi Ranjan , A.K. Goel, S.K. Jindal and S. K. Agarwal
In vitro embryo production.
The oocytes were collected from ovary by slicing
technique in a petridish containing oocyte
collection media (OCM). A total of 2239 goat
oocytes were processed for IVMFC. The oocytes
surrounded in a compact cumulus mass with an
evenly granulated cytoplasm were selected
under stereozoom microscope. Selected oocytes
(2239) were washed two or three times in Oocyte
Holding Medium (OHM) and allowed for
maturation in 50l drop of IVM medium in
35mm10mm Petri dishes for 27
hours in a CO2 incubator maintained at 38.5 C,
5% CO2 and 90% humidity. The matured oocytes
4.06
4.2
4.44
3.5
4
4.5
5
11-12 m 13-14m 15-16m
Age groups
P
r
o
g
e
s
t
e
r
o
n
e

(
n
g
/
m
l
)

S eries 1
5.21
5.01
4.78
5.04
4
4.5
5
5.5
6
30d 60d 90d 120d
P regnanacy (in days)
P
r
o
g
e
s
t
e
r
o
n
e

(
n
g
/
m
l
)

S eries 1
CIRG

25
were separated from cumulus cells. Fresh semen
samples were obtained by an artificial vagina
from a fertile purebred Sirohi bucks and sperm
were capacited in TALP medium supplemented
with heparin, BSA or 10% FBS and antibiotics.
Fertilization drop containing oocytes were
inseminated with 25 to 50 l of final diluted
semen (1x10
6
sperm / ml). The oocytes were
culture in culture medium TCM-199 for embryo
development. The overall 2-cell, 4-cell, 8-16-cell,
morula, blastocyst and hatched blastocyst
production from in vitro fertilization of matured
oocytes were 21.40%, 25.81%, 30.71%, 15.84%,
5.55% and 0.98%, respectively
Parthenogenetic embryo production:
IVF Blastocyst
Ethanol Activation: The oocytes were collected
from each ovary in a petridish containing oocyte
collection media (OCM). Selected oocytes (2140)
were washed two or three times in Oocyte
Holding Medium (OHM) and allowed for
maturation in 50l drop of IVM medium Petri
dishes for 27 hours in a CO2 incubator. The
matured oocytes (1988) were activated 7%
ethanol in mCR2aa medium for 5 min followed
by treatment with 2.0 mM DMAP for 4 hr in
mCR2aa medium. After 4 hr, the oocytes were
washed 5 to 10 times in the culture medium and
were divided in to two groups. Group 1 (n=1746)
oocytes cultured in 50 l drop of RVCL for 12
days. Group 2 (n=242) oocytes cultured in 50 l
drop of mCR2aa medium for 12 days.
Table : Parthenogenetic embryo development in two different media

S. No. Media 2-cell
(%)
4-cell
(%)
8-16-
cell
(%)
Morula-
cell (%)
Blastocyst-
cell (%)
Hatched
Blastocyst-
cell (%)
1. RVCL 16.84 19.76 29.60 28.72 3.11 1.94
2. mCR2aa 10.08 28.57 39.49 21.84 0.00 0.00

Ionomycin Activation: The oocytes were
collected from each ovary in a petridish
containing oocyte collection media (OCM).
Selected oocytes (2339) were washed two or
three times in Oocyte Holding Medium (OHM)
and allowed for maturation in 50l drop of IVM
medium in Petri dishes for 27 hours in a CO2
incubator. After maturation for 2427 h, oocytes
were stripped off their cumulus cells. The
matured oocytes (2192) were activated 5 M
Ionomycin in mCR2aa medium for 5 min
followed by treatment with 2.0 mM DMAP for 4
hr in mCR2aa medium. After 4 hr, the oocytes
were washed 5 to 10 times in the culture medium
cultured in 50 l drop of mCR2aa medium for 12
days. The overall 2-cell, 4-cell, 8-16-cell, morula,
blastocyst and hatched blastocyst production
from in vitro fertilization of matured oocytes
were 46.02%, 33.71%, 10.58%, 11.04%, and 0.57%,
respectively.

Parthenogenetic Blastocyst
CIRG

26
Intra Cytoplasmic Sperm Injection followed
by activation for production of fertilized
embryos
Intracytoplasmic sperm inj ection (ICSI) is a
technique for assisted reproduction (ART)
consisting of the microinj ection of a single sperm
across the membrane of a metaphase II oocyte
leading to fertilization. The potential
applications of ICSI in animals include its use
with sperm obtained post-mortem, with samples
that have low motility or with samples from
individuals of high genetic value or special
features, such as transgenic animals or
endangered species. With respect to oocytes, ICSI
can be useful in the fertilization of oocytes with
alterations preventing the normal process of
fertilization, such as cryopreserved oocytes, in
vitro matured oocytes and oocytes obtained from
prepubertal females.
A total of 277 cumulus oocyte complexes (COCs)
were collected from goat ovaries. In-vitro
matured oocytes were evaluated for maturation
on the basis of cumulus expansion.
Morphological matured oocytes (n=146) were
selected, denuded and randomly divided into
two groups. Group 1 (n=200) in vitro matured
oocytes were inj ected with GFP followed by
activation with 5 m ionomycin for 5 min. and
cultured in RVCL medium. Group 2 (n=70) in
vitro matured oocytes were inj ected with sperm
followed by activation with 5 m ionomycin for
5 min. and cultured in RVCL medium. The
cleavage rate in groups 1 and 2 were 0.01% &
34.28%, respectively.
Fertilized embryo produced through ICSI
Goat fetal skin fibroblast cell monolayer
preparation
The uterus containing 3-4 cm fetus was
aseptically collected from local slaughter house
and transported to laboratory within 1 hour. The
whole organ was washed thoroughly in sterile
normal saline solution (NSS) (38
0
C)
supplemented with gentamycin (50g/ml). In
brief, collected fetal skin samples was chopped
and trypsinised. Finally fibroblast cells were
bathed with DMEM media and cultured in 25
cm
2
of tissue cell culture bottle at 37
0
C, 5% CO2
level and 90% relative humidity in CO2
incubator. Media was changed in every 72 hours
interval and subculture was made as per
requirement.

Fibroblast cells Fibroblast cells monolayer
The fibroblast cells were plated in 25 cm
2
tissue
cell culture bottle in DMEM with FBS. Fresh
media was replaced every 72 hours for
maintaining the culture. The media was replaced
with trypsin (0.25%) solution sufficient to cover
the entire surface and incubated for 5 minute in
CO2 incubator. The cells were centrifuge for 5
minute at 1000 rpm. Supernatant was discarded
and 3.5 ml fresh DMEM media was added. The
media with cells were transferred to 96 well
plates or 6 well plates for setting monolayer or
store at -80
0
C for future use. Fibroblast
monolayer derived after second subcultures
were used for growing of blastomere culture.
Once confluences, the mitotic growth was
blocked by Mitomycin-C treatment for 4 hours.
CIRG

27
The mitomycin-C (10g/ml) was removed by
washing the monolayer cells 3-4 times with
warm (37
0
C) 1X PBS and then replaced with fresh
media.
Storage of fibroblast cells:
The media was replaced with trypsin (0.25%)
solution sufficient to cover the entire surface and
incubated for 5 minute in CO2 incubator. The
solution was pipetted in and out gently for
complete detaching the cells from culture bottle
and placed into 15 ml sterile tube containing
DMEM media. The cells were centrifuge for 5
minute at 6000 rpm. Supernatant was discarded
and pallete was dissolved in 3ml freezing media
(DMSO-10%; FBS-30% and DMEM- 60%) and
stored in cryovial contain 1-1.5 million cells/ml at
-80
0
C for future use.
Effect of fetal sex on confluences of goat fetal skin
fibroblast cell monolayer: The passaging of goat
fetal skin fibroblast cell monolayer was done
when confluences attained more than 80% with
trypsin (0.25%) solution. It was observed that
female fetal skin fibroblast cell monolayer
attained the same percent of confluences 24 h
earlier than to male fetal skin fibroblast cell
monolayer. The female fetal skin fibroblast cell
monolayer has taken 96 h to attain more than 80
percent of confluences. The male fetal skin
fibroblast cell monolayer has taken 120 h to attain
more than 80 percent of confluences
Development of embryonic stem cell colonies
by hanging drop culture method
For generation of embryonic stem cell colonies,
blastomere of 8-16 cell stage parthenogenetic
embryos were removed from IVC drops and put
into warm PBS for washing. After washing,
embryos were shifted to proteinase-K (0.02%)
drops and the thinning or dissolution of zona
pellucida was observed under zoom stereo
microscope. After thinning or dissolution of zona
pellucida, the proteinase-K activity was
neutralized by addition of media containing FBS
(20% FBS in DMEM media). The clumped
blastomere cells were washed in 4-5 drops of
stem cell culture media and kept in hanging drop
of stem cell culture media (20 uL) and kept in CO2
incubator for growth. The good quality ES
colonies were isolated from the culture with
different media, washed in PBS and stored at -
80
0
C in PBS for molecular studies. When the
clumped blastomere divided and formed 100-150
cells, the colonies were eluted by 0.25% trypsin
EDTA solution and further cultured in respective
hanging drop culture condition. Parthenogenetic
embryos of 8-16 cell (32) and morula (28) were
used for embryonic cell colony formation. The
percentage of embryonic cell colony formed was
28.12 and 25.00 from 8-16 cell and morula stage
derived embryos, respectively.
Table- Embryonic stem cell colony formation by hanging drop culture method (Mean SE)
Types of
embryos
Stage of
embryos
No. of embryos
used
No. of
embryonic cell
colony formed
Percentage of
embryonic cell
colony formed (%)
Parthenogenetic 8-16 cell 32 9 28.12
Morula 28 7 25.00
Development of embryonic stem cell colonies on
goat fetal fibroblast monolayer
For derivation of embryonic stem cell colonies,
blastomere of 8-16 cell staged parthenogenetic
embryos were removed from IVC drops and put
into warm PBS for washing. After washing,
embryos were shifted to proteinase-K (0.02%)
drops and the thinning or dissolution of zona
pellucida was observed under zoom stereo
microscope. After thinning or dissolution of zona
pellucida, the proteinase-K activity was
neutralized by addition of media containing FBS
(20% FBS in DMEM media). The clumped
blastomere cells were washed in 4-5 drops of
stem cell culture media and kept in CO2
incubator for growth. The undissociated
clumped blastomere were cultured in wells on
mitomycin-C inactivated goat fetal fibroblast
monolayer at 38.5
0
C, 5% CO2 and 90% relative
humidity in CO2 incubator in different media
containing different growth factors. The good
quality ES colonies were isolated from the
culture with different media, washed in PBS and
stored at -80
0
C in PBS for molecular studies.
When the clumped blastomere divided and
formed 200-300 cells, the colonies were eluted by
CIRG

28
0.25% trypsin EDTA (Gibco, 25200) solution and
further cultured in respective culture condition.
Parthenogenetic embryos of 8-16 cell (20) and
morula (18) were used for embryonic cell colony
formation. The time taken for their attachment on
goat fetal fibroblast monolayer was 72-96 hrs.
The percentage of embryonic cell colony formed
was 65.005.16 and 61.114.77 from 8-16 cell and
morula stage derived embryos, respectively.
There was no significant difference in the
formation of embryonic cell colony between 8-16
cell and morula stage derived embryos on goat
fetal fibroblast monolayer (P<0.05). Embryonic
cell colonies were further passage up to third
passage on goat fetal fibroblast monolayer and
stored in liquid nitrogen for further use or
molecular analysis.
Table 1 Embryonic cell colony formation on goat fetal fibroblast monolayer in caprine (Mean SE)
Types of
embryos
Stage of
embryos
No. of
embryos
used
No. of
embryonic
cell colony
formed
Time taken
for
attachment
(Hrs)
Percentage of
embryonic cell
colony formed
(%)
Parthenogenetic 8-16 cell 20 13 72-96 65.005.16
a

Morula 18 11 72-96 61.114.77
a

These embryonic cell colonies will used for
molecular studies. The attachment rate of
porcine embryonic cells on feeder layer was
highest from in vivo embryos followed by
parthenogenetically activated and IVF embryos
(Ock et al., 2005). It has been reported that all the
in vitro produced embryos are not able to
propagate and give stem cells, when blastomere
cells were cultured (Hassan-Hauser et al., 1990;
Swain, 2006).

8-16 cell derived ESC Colony-P-0 (7
th
days) 8-16 cell derived ESC Colony -P-0 (5
th
days)

Morula stage derived ESC Colony-P-0 (7
th
days)
Morula stage derived ESC Colony-P-0 (7
th
days)
Parthenogenetic embryonic cell colony on goat fetal fibroblast monolayer during different days of culture in caprine (100X)

CIRG

29
NAIP Project: Developmental potency of parthenogenetic goat
embryos
S. D. Kharche , A. K. Goel and S. K. Jindal
Parthenogenetic embryo production
The oocytes were collected from the ovary (916)
in a petridish containing oocyte collection media
(OCM. Selected oocytes (2162) were washed two
or three times in Oocyte Holding Medium
(OHM) and allowed for maturation in 50l drop
of IVM medium in Petri dishes for 27 hours in a
CO2 incubator maintained at 38.5 C, 5% CO2 and
90% humidity.
After maturation for 2427 h, oocytes were
stripped off their cumulus cells by treatment
with 0.1% hyaluronidase. These oocytes were
activated either by 7% ethanol in mCR2aa
medium for 5 min followed by treatment with 2.0
mM DMAP for 4 hr in mCR2aa medium. After 4
hr, the oocytes were washed 5 to 10 times in the
culture medium and cultured in 50 l drop of
mCR2aa/RVCL. The overall cleavage rate, 2-cell,
4-cell, 8-16-cell, morula, blastocyst and hatched
blastocyst production from parthenogenetically
activated oocytes were 58.81%, 15.15%, 20.46%,
34.76%, 25.34%, 2.56% and 1.71%, respectively.
Transfer of Parthenogentic goat Embryos
Parthenogenetic embryos produced as above of
different stages were surgically transferred into
twenty one recipients. In six recipients 2 to 4 cell
parthenogenetic embryos (4-6 embryos in each
recipient) were transferred into the fallopian tube
through infundibulum epsilateral to the corpus
luteum whereas other eight recipients received
16 to 32 cell stage parthenogenetic embryos (4-6
embryos in each recipient) at the tip of the uterine
horn epsilateral to the corpus luteum. Oestrus
detection following embryo transfer in recipients
was carried out with the teaser buck twice a day
regularly for monitoring the onset of oestrus.
Following embryo transfer, in vivo survivability
of parthenogenetic embryos into the recipients
were also monitored by using B-mode ultra
sonography at different days of pregnancy by
viewing anechoic fluid filled cavity. Out of 21
recipient one recipient was pregnant at day 23
rd

of pregnancy showing anechoic fluid filled cavity
(Goat 180). The fetal fluid was reabsorb and no
anechoic area was present on day 29
th
of
pregnancy. Another recipient was pregnant up
to 28
th
day of post transfer (Goat 153). The fetal
fluid was reabsorb and no anechoic area was
present on day 40
th
of pregnancy. Rest of the
recipients did not shown any sign of pregnancy
following ultrasonography.

DST project : To analyse genetic trait and expression analysis of goat esr1 gene for
buck fertility and sperm quality
Sonia Saraswat, S. D. Kharche, P. K. Rout
A total of 70 collections were done from bucks of
Jamunapari (n=28) and Barbari (n=35) aged
between 2-5 year, maintained at experimental
shed of GGB Division, CIRG, Makhdoom, Farah.
Before collection the bucks were trained for a
month with teasure doe. The semen was
collected by means of artificial vagina having
internal temperature of 40
0
C in the morning
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30
hours between 7.30 to 8.30 AM. Before semen
collection, two false mounts were given to each
buck to improve the semen quality and to obtain
maximum number of sperm cells. The semen was
collected from each buck and immediately after
collection, semen was evaluated for its various
attributes like volume, colour, consistency and
mass activity, after dilution with Tris egg yolk
progressive motility, live & dead and
abnormality was estimated. The average and SE
values were determined for ej aculates of
j amunapari bucks, volume (0.8920.17); mass
activity(3.440.68); progressive motility
(77.615.52); live% (86.1617.23) and
abnormality(1.80.36). Similarly, the average and
SE values were also determined for ej aculates of
Barbari bucks, volume (0.690.12); mass activity
(4.430.75 (); progressive motility (82.2913.90);
live% (89.49 15.12) and abnormality (0.74 0.13)
respectively.
Furthermore, DNA isolation was done from
blood samples. The O.D. of DNA samples was
checked using biophotometer, the concentration
of the samples which lies between 1.7 to 1.9g/ml
were used for PCR. Primers for gene ESR were
standardized. The standardized PCR
programme was comprised of following steps-
Initial denaturation at 95C for 5 min (step1),
followed by 35 cycles of denaturation at 95C for
30sec (step 2), annealing at 62C for 30 sec (step
3), extension at 72C for 45 sec (step 4) and a final
extension at 72C for 7 minute in a gradient
thermal cycle.

Comparative study on different structures of goat shelters under farm conditions
N. Ramachandran, B. Rai, S K Jindal, M. K. Tripathi, A.K. Dixit, and Souvik Paul

The prevailing climate changes are impacting
goat production system due to severe cold and
harsh hot conditions, which generated necessity
to search alternate and efficient housing systems
for goat in minimizing production losses. The
height of the slatted floor is normally
recommended at 5 feet to have easy cleaning but
varies widely depending on the agro climatic
conditions and production system. The fixed
height floors cannot be used for multiple
purposes, whereas adj ustable slatted floor can be
used in protecting the animals from winter and
hot weather conditions. Moreover, the most
suitable height of slatted floor for goats is also not
known. Therefore a small adj ustable slatted floor
(15X 20size) is constructed using Mirandi wood
slats having 2 width and 1thick with 1.5 cm
gap between slats. The height of the floor is
designed to have adj ustment at 1.25, 2.5, 3.75, 5.0
feet from ground level using nut and bolts
system in the wooden pillars. The designed
slatted floor will be widely utilized to record the
growth of kids, milk yield of lactating does at
different heights giving due consideration on
microclimate, welfare of goats, worm load (EPG),
labor saving etc so as to assess the necessity of
slatted floor before recommending for farmers in
dry regions.
An experiment was initiated using thirty post
weaned Jakhrana male kids adj usted for age,
type of birth and body weight, and randomly
divided in to two equal groups of 15 each and
housed/managed on slatted floor Vs kutcha floor
in goat farm of the Institute. The live weight
change is being monitored at weekly intervals for
two consecutive days to assess the daily gain in
CIRG

31
kids. Animals were also observed for the
incidence of diarrhea and other illness, if any.
Since the beginning of experiment, the group
feed intake (mash/kid pellet, bhusa, green
fodders/tree leaves) is being recorded daily. The
feed samples are being collected at fortnightly
intervals for estimation of dry matter and
proximate composition. The blood samples are
collected at monthly intervals and the ser um
samples are being stored for hormone and
biochemical assays. The faecal samples are
processed for EPG counts once in two months.
The average initial body weight at the start of the
trial was 10.15 Kg in both the groups. Kids on the
slatted floor had finishing live weight of 19.83 kg
in comparison to 19.40 kg in kids on kutcha floor
with the mean body weight gain of
8.04 and 7.29 kgs, respectively after 11 weeks of
trial/ at 6 months of kids age.
Feed intake improved linearly in kids with
progress of experiment. The kids of both group
had an average dry matter intake of 643g/h/d at
91-180 days of age. However, the weight gain
was 4.53% higher in kids under slatted floor than
that under kutcha floor with the overall feed
conversion efficiency of 17.48 and 16.74%,
respectively. Under present experimental
protocol, kids on slatted floor had higher feed
efficiency at all stages of growth except 5-6
months. Kids on slatted floor consumed 5.06,
6.58, 7.32 and 6.43 kg feed while kids on kutcha
floor consumed 6.12, 7.75, 7.04 and 7.05 kg feed
for each kg gain during 3-4, 4-5, 5-6 and 3-6
months of age, respectively.
Effect of floor on intake, growth and feed
conversion efficiency in Jakhrana kids
Parameters Kutcha
Floor
Slatted Floor
Feed DMI/ kid/day (g)
3-4 months 517 548 (+5.66%)
4-5 months 668 658 (-1.52%)
5-6 months 712 700 (-1.71%)
Overall 643 643 (+0.00%)
Average daily gain (g)
3-4 months 104.44 114.21
(+8.55%)
4-5 months 106.73 118.75
(+10.12%)
5-6 months 108.21 102.80 (-
5.26%)
Overall 106.65 111.71
(+4.53%)
Feed Efficiency
3-4 months
kg feed DMI/kg
Gain
6.12 5.06
Per cent (%) 19.94 20.73
4-5 months
kg feed DMI/kg
Gain
7.75 6.58
Per cent (%) 15.93 17.17
5-6 months
kg feed DMI/kg
Gain
7.04 7.32
Per cent (%) 15.14 14.65
Overall
kg feed DMI/kg
Gain
7.05 6.43
Per cent (%) 16.74 17.48

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Holistic approach for improving livelihood security through livestock based farming
system in Barabanki and Raebareli districts of U.P
B. Rai, Ashok Kumar, M.K.Singh and H. Dixit
Goat rearing practices prevailing in 10 villages of
Barabanki district of Eastern Uttar Pradesh was
studied under NAIP proj ect (Comp-3) during the
year 2013-14. The survey during initial years
2009-2011, indicated that the goat feeding
practices in this region was traditional and
primarily based on zero-input system. Farmers
are keeping large animals along with goats for
milk and other products. The main stay of goat
feeding was grazing followed by feeding tree
leaves and kitchen waste. The grazing area is not
demarcated and usually goats are grazed in the
fields, bunds, wastelands and roadsides. The tree
leaves commonly used are Neem, Pakar, Gular,
Babul alongwith seasonal grasses. The field
study indicated that pasture land grazing (17%)
is not common and most of the goats (66%)
grazed in wasteland (table). Colostrum feeding
of kids was at satisfactory level (78.38 %)
however, feeding of mineral mixture was at
lower side (29.41%). The stall feeding was
practiced by small number of the farmers
(6.25%). The goat in production phases
(pregnancy and milking) and kids during growth
need higher amount of DM, protein and mineral
supplements for better production. It was
suggested that the goat keepers shoud go for
supplementary feeding during crucial
production phases. The farmers can easily go for
supplementary feeding of productive goats
(Grazing+300-500g dry fodder +150-250g
concentrate mixture+5g mineral supplement per
day) for better growth and production. The
farmers may collect seasonal grasses and tree
leaves dry it during sunny days and may keep
the dried bundles for its further use in feeding
goats during lean periods. Since in traditional
goat rearing the concept of feeding goats at the
farmers doorstep is not common and farmers
consider that merely grazing of goats is enough.
The supplementary feeding of goats must be
advocated among the goat keepers to enhance
the productivity of goats in this region.
Simultaneously plantation of fodder trees in
grazing areas may be undertaken by state
department and developmental agencies to
ensure fodder in the form of tree loppings for
goats in this region.
Feeding practices of goats under field
condition
Particulars Yes (%) No (%)
Grazing on pasture land

54 46
Grazing on waste land 66 34
Supplementation of tree
leaves
87 13
Supplementation of kitchen
waste
91 09
Colostrum feeding to new
born kid
78 22
Feeding of mineral mixture 30 70
Stall feeding of animals 07 93
Impact analysis:
The impact analysis of goat based interventions
indicated positive impact on goat rearing
venture in both Barabanki and Raebareli . The
data shows that there was considerable
improvement in body weight at 6 month,
lactation yield and reduction in mortality rate as
compared to baseline performance of goats. The
Sirohi and Barbari breeds distributed among
adopted farmers fetched better price and gross
income from goat rearing increased at the rate of
Rs.2000.00 per goat due to better growth and
milk production in both the breeds. The
improved breed, healthcare and integration of
goat rearing with backyard poultry and
horticulture made it possible to get more
remuneration from goat keeping in this region.
Impact analysis of NAIP Com-3 project
Particulars Baseline
(2008-09)
(2013-
2014)
Average flock size 3.4 5.0
Number of breeding
bucks
12 49
Body weight at 6 month
(kg)
8.4 12
Milk yield per day (ml) 502 705
Lactation length (day) 102 142
Mortality (%) 14.7 8.4
Price of adult buck (Rs.) 2412 4407
Price of adult doe (Rs.) 2218 4002
Gross income per goat
(Rs.)
2356 4338

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Nutrition, Feed Resources & Production Technology Division
Development of feed resources on poor lands for goats
Prabhat Tripathi, M.K. Tripathi, Ravindra Kumar, U.B. Chaudhary
Utilization of Leucaena leucocephala leaves as sole
roughage source in kids.
Incorporation of Leucaena leucocephala foliage
(LLF) provided alternative in tropical ruminant
production due to nutrients richness, especially
of protein, vitamins, and minerals. Present study
evaluated intake, performance and mimosine
tolerance of goat kids on LLF feeding as only
source of roughage. Kids were fed ad-libitum LLF
and concentrate supplement 2% of LW on fed
basis.

Control
group (n=9)
Leucaena
lecocephala
group (n=9)
Initial live
weight (kg)
7.18 7.34
Final live
weight (kg)
17.76 17.84
Total gain
(kg)
10.58 10.50
ADG (g/d) 75.56 75.00

Eighteen Barbari kids of 54+10.23 days of age and
7.25+0.81 kg live weight of either sex were
randomly divided in to two equal groups. The
kids of control group received sorghum fodder,
while kid of other group received Leucaena
leucocephala foliage in replacement of sorghum as
green fodder. Feeding experiment lasted for 140
days. Mimosine content of Leucaena leucocephala
foliage ranged from 14 to 57 g, with a mean 33.28
g/kg dry matter at different stages of growth. The
CP content of Leucaena leucocephala was 21 %.
Growth performance of the both kids groups was
identical. Therefore, Leucaena leucocephala foliage
may be used as sole green fodder on goat feeding
Effect of protein sources of on intake,
digestibility and milk production of lactating
goats
Experiment utilized mustard oil meal, urea and
guar korma in replacement of conventional
linseed oil cake as protein supplement in
concentrate mixture of lactating goats feeding in
economizing cost of feeding. Twenty seven
lactating Barbari goats during their second week
of parturition were randomly divided in to t hree
equal groups of nine in each. Each goat received
concentrate pellet with 16 % crude protein, the
pellet fed to control goats contained linseed oil
cake, MOM group contained mustard oil meal,
while NPN group contained urea and guar
korma as protein sources. The goats were also
offered ad-libitum gram straw and green fodder
at 2 kg per goat/day.
Total daily dry matter intake (DMI) was lower
(p<0.05) in control (904 g) than occurred in MOM
(999 g) and NPN (1036 g) groups of goats. Similar
crude protein intake (CPI) was similar through
straw, green fodder and concentrate, while total
CPI was higher (p<0.05) in MOM and NPN
group of goats in comparison to control goats.
Dry matter, organic matter, crude protein,
hemicelluloses and total carbohydrates
digestibility were similar among three goat
groups, however neutral detergent fiber, acid
detergent fiber and cellulose digestibility were
lower (P<0.05) in MOM group, which consumed
concentrate pellet that contained mustard oil
meal as protein source. Milk production
performance of goats ranged from 755 to 839 ml/
day, which was significantly lower by 5.6 and
11.1 % than occurred in MOM and NPN group of
goats. It is concluded that mustard oil meal, and
combination of urea and guar korma can replace
conventional protein supplement linseed cake in
lactating goats feeding, with improved lactating
performance.
Evaluation of L. multiflorum for its Fodder
Production Performance
The L. multiflorum was grown during the rabi
season as sole crop as well as in combination. it
is evaluated for production parameters as well as
chemical composition. It can be successfully
grown with Oat, Berseem etc crops. It contained
12.86 % CP, 48.92% NDF and 7.22 lignin. The in-
vitro dry matter digestibility was 76.57% with
energy value17.628MJ/kg dry matter.

CIRG

34
Nutrient content of Lolium Multiflorum (Rye grass) for its fodder value
Fodder Green
fodder
t/ha

DM OM CP EE NDF ADF Cellulose Lignin TCOH
Berseem 40.3 9.2 84.30 22.96 4.69 41.
22
38.14 28.29 9.62 56.63
Oat 86.22 8.13 82.70 12.46 3.31 53.34 40.40 35.84 4.20 66.92
Rye Grass 64 8.82 82.08 12.86 2.73 48.92 45.47 36.74 7.22 66.48
Berseem
+ Rye grass
44.88 7.51 79.56 15.91 2.91 52.36 29.23 26.87 1.82 62.73
Lucerne
+ Rye grass

38.66 9.1 80.99 14.81 3.46

47.43 35.95 23.51 6.96 65.70
Oat
+ Rye grass
72.00 8.03 83.29 12.51 2.22 52.95 29.97 22.35 7.11 67.55
Evaluation of Commelina bemghalensis and Seteria sp. for fodder value
These two plant species were identified for their
natural production capacity in ravine area. The
biomass was collected from the waste land j ust
after monsoon season, The collected biomass was
conserved as hay. in-vitro as well as chemical
evaluation were carried out. C. benghalensis and
Setaria sp. were recorded with 14.875 and 15.57
MJ/kg energy values. The in- vitro dry matter
digestibility were 58.83 and 40.6 % respectively.

Fodder CP OM EE EE NDF ADF Lignin TCOH
Setaria sp.( Fox tail weed) 11.38 85.30 1.51 1.51 63.79 50.55 10.79 83.79
Commelina benghalensis 6.56 85.52 1.38 1.38 73.91 54.03 10.00 77.58
Integrated Nutrient Management in Nursery Seedlings
Treatments i.e.1% Urea+ ZnSo4, 2% Urea,
VermiWash, VermiWash + ZnSo4+ FeSo4, Soil
application of NPK , 0.2 % spray of ZnSo4,
FeSo4+ Boron and control were applied on
nursery grown seedlings of Acacia nilotica, P.
cineraria, S. Cummunis, F. religiosa, F. glomerata, M.
alba, Azadirachta indica, F. lacor. Some buring
spots were observed with urea solution spray in
case of M. alba. Maj or inj uries were observed in
case of F. religiosa and F. glomerata. Seedlings of
Acacia nilotica showed positive response towards
high dose of NPK . All other plants species
observed up to 90-100% mortality.

Network program on estimation of methane emission under different feeding systems
and development of mitigation strategies
M.K. Tripathi, Prabhat Tripathi, Ravindra Kumar, U.B. Chaudhary and P.K. Rout

In-vitro fermentation experiments for 24 h were
carried out to determine methane production
potential of different feeds used goat feeding.
Methane mitigation experiments were also
carried out using different protein sources in
concentrate pellet.
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35
Methane production from different leaves
Leaves of seven bio-resources namely Aloevera
(Aloe barbadensis), Banana (Musa paradisiacal),
Aanar (Punia granatum), Meetha neem (Murraya
koenigii), Mehendi (Lowsonia inermis), Biskhapra
(Boerhavia diffusa) and Khej ri (Prosopis cineraria)
were evaluated. Nutrient content of all the bio-
resources were different (p<0.001), and the gas
production varied 57.7 to 161.7 ml/ g DM, which
was the highest (p<0.001) in Aloevera and the
lowest in Khej ri leaves. Although, gas
production was different among all feed
resources, however gas production for each g
DM fermented was similar in Banana, Aanar,
Meetha neem and Mehendi leaves. Methane
concentration in gas ranged from 14 to 21.2 %,
where as ME (MJ/kg DM) varied from 4.75 to
7.11. Methane production in g/ kg DM and g/ kg
fermented DM ranged from 6.7 to 18.9 g and 10.5
to 22.83 g respectively. Similarly, energy loss in
the form of methane followed the trend of gas
production, which ranged from 11.4 to 17.1 % of
digestible energy. Different feed resources with
varying nutrient contents have varying
fermentability and methane production
potential.
Table: Nutrient content and methane production of different leaves
Alovera Banana Aanar Meetha
Neem
Mehendi Vishkhapra Khejri SEM P-
value
Nutrient contents (%)
DM 2.21g 16.29e 64.26a 37.68c 27.83d 10.31f 44.83b 4.473 <0.001
OM 77.48f 88.43c 91.84b 85.26d 93.53a 81.25e 90.69b 1.229 <0.001
CP 9.04f 14.29d 13.39e 15.08c 15.86b 7.72g 17.62a 0.752 <0.001
Fat 2.07f 6.49b 8.15a 5.50c 4.89d 1.44g 3.50e 0.498 <0.001
NDF 24.07f 65.22a 26.04e 41.22c 16.07g 38.35d 48.24b 3.45 <0.001
ADF 16.32f 30.75b 17.90e 21.17d 11.83g 26.25c 39.95a 1.996 <0.001
Cellulose 13.25e 25.27a 9.37f 14.39d 8.42g 17.91c 20.51b 1.257 <0.001
Lignin 3.02f 4.12e 8.56b 6.80d 2.77g 7.79c 19.10a 1.167 <0.001
Gas production
ml/g DM 161.7a 93.3cd 125.0b 86.7c 128.3b 77.5d 51.7e 7.804 <0.001
Ml/g DDM 186.4a 147.5b 152.1b 130.5b 141.5b 101.2c 79.3d 2.876 <0.001
Methane
(%)
17.23b 16.44b 17.33b 16.52b 14.70b 21.21a 20.39a 0.544 0.001
DM Digestibility (%)
86.75b 63.30f 82.15c 66.45e 90.66a 76.57d 65.20ef 2.30 <0.001
ME (MJ/kg DM)
7.11a 5.55c 6.36b 5.44c 6.60b 4.75d 4.61d 0.201 <0.001
Methane Production (ml/g DM)
ml/ g DM 26.44a 13.85cd 20.29b 12.75de 17.04bc 15.62cd 9.58e 1.214 <0.001
ml/ g DDM 30.49a 21.79b 24.68b 19.20bc 18.80bc 20.40bc 14.70c 1.184 0.001
g/kg DM 18.93a 9.92cd 14.53b 9.13de 12.21bc 11.19cd 6.86e 0.869 <0.001
g/kg DDM 21.83a 15.61b 17.68b 13.75bc 13.57bc 14.61bc 10.51c 0.848 0.001
Methane energy loss
% of GE 14.81a 9.92b 12.69a 9.28b 10.29b 13.07a 8.27b 0.545 <0.001
% of DE 17.07a 15.61ab 15.44ab 13.97abc 11.35c 17.07a 12.69bc 0.567 0.015

Methane production in concentrate pellet feed
Methane production potential of the three
concentrate pellet feed (16 % CP) was estimated
in which different protein sources were used.
The connectional protein supplement was used
at 25 % in control pellet feed, linseed cake was
replaced (w/w) by mustard cake and in another
pellet feed guar korma (5.7%) and urea (1.3%)
were used in replaced cakes. The methane
production of the three feed varied from 38.87 to
47.08 ml/ g digestible DM. Mustard cake
inclusion reduced methane production by 21.09
%, whereas the concentrate pellet containing
guar korma and urea produced 4.83 % less
methane in comparison to linseed cake included
pellets.
CIRG

36
Concentrate pellet
feed
Gas (ml/g
DM)
IVDMD
%
CH4
%
CH4(ml/g
DDM)
CH4 energy loss
(MJ/kg DDM)
Linseed cake (25%) 241.7 79.2 18.09 47.08 1.87
Mustard cake (25%) 248.3 78.0 15.29 38.87 1.55
CH4 reduced 21.09%
Guar korma (5.7%) +
Urea (1.3%)
262.5 81.8 16.69 44.91 1.78
CH4 reduced 4.83%
Methane production of Subabool leaves and
its combination with concentrate
Methane production of Subabool leaves was
estimated as sole and with 50 % concentrate feed.
The concentrate pellet was containing CP 18%
whereas subabool leaves having CP 26%.
Subabool leaves produced 22.54 ml/methane in
each g of digestible dry matter, whereas
concnetarte pellet produced 52.14 ml methane.
The substrate, which contained subabool leaves
and concentrate in a 50 :50 ration produced
methane 1.44 ml/ g digestible substrate.
Similarly, methane energy loss (MJ/kg digestible
DM) followed the similar trend. Subabool leaves
produced less methane than that occurred in
concentrate pellet. In, general concentrate feed
producing less methane in comparison to
fodders, the reverse trend of methane production
in the present study show that the quality of feed
especially the protein content is responsible for
the level of methane production.
Gas (ml/g
DM)
IVDMD
%
CH4
%
CH4 (ml/g
DDM)
CH4 energy loss
(MJ/kg DDM)
Subabool leaves 112.5 76.48 20.35 22.54 0.89
Concentrate pellet 216.6 70.8 20.49 52.14 2.08
Concentrate +
Subabool leaves (50%50)
160.0 77.58 20.80 36.06 1.44

Therefore, methane production potential (MPP)
of different feeds was varied from 13.4 to 36.4 ml/
g digestible DM. The Energy loss as methane was
ranging from 0.53 to 1.45 MJ/kg Digestible DM.
Replacement of linseed cake by mustard cake in
concentrate pellet reduced methane production
by 21%. The quality of fodder, especially the CP
level is responsible in determining the MPP of
the feed resources/ compound feeds.
Development of complete feed for environmentally and economically sustainable goat
production
Ravindra Kumar, P. Tripathi, U. B. Chaudhary, and R. B.Sharma

Ponds were prepared at CIRG, Makhdoom for
the cultivation of Azolla. Successful cultivation
was carried out. Azolla was harvested, dried and
stored for further analysis and experimental
feeding.

Fig 1: Cultivation of azolla in the pond
CIRG

37
Nutrient composition and digestibility of azolla
was studied using in vitro technique to evaluate
it as a protein supplement for the goats. The dry
matter content of azolla was 7.10 %. Among
proximate principles per cent ash, crude protein,
ether extract and crude fibre were 20.31, 17.3, 2.71
and 12.02 respectively on dry matter basis .The
per cent NDF and ADF was 46.90 and 31.73
respectively. Total gas production (ml/g DM)
and methane production (ml/DM) was 78.49 and
11.96 respectively. The dry matter digestibility
(%) and organic matter digestibility (%) was
83.15and 84.03 respectively. Microbial protein
(mg) and partitioning factor was 76.57 and 5.25
respectively. Five feeds were formulated by the
replacement of concentrate mixture by azolla
meal at 0 (F1), 25(F2), 50 (F3), 75(F4) and 100 (F5)
level. These feeds were evaluated for methane
emission and digestibility with goat rumen
liquor as inoculums in an in vitro gas production
test. Gas production (ml/g dry matter and
ml/digestible dry matter) significantly get
reduced with increasing proportion of azolla.
There was significant (P< 0.05) difference in
methane production between different
formulated feeds. Methane production (ml/Dry
matter) was highest in F1 (27.54) followed by F2
(27.18), F3 (21.23), F4 (17.37) and lowest in F5
(14.77).Similar trend was observed with methane
production (ml/ digestible dry matter). The dry
matter digestibility (%) ranged from 63.19 to
51.69 being significantly highest in F1 (63.19)
followed by F2 (61.19), F3 (58.83), F4 (51.33) and
F5 (51.69). Similar trend was observed with
organic matter digestibility (%) of different
formulated feeds. The values ranged from
65.21(F1) to 54.01(F5).The dry matter and organic
matter digestibility of F1 (63.19, 65.21) and F2
(61.19, 64.38) feeds were statistically similar.
With increasing percent of azolla meal in the feed
formulation a decreasing trend of dry matter and
organic matter digestibility was reported.
Body weight gain and feed intake in different group of goats
Attributes Group A Group B
Initial body weight 11.190.56 11.420.39
Final body weight 14.440.93 15.800.68
ADG (g) 56.6010.78 78.1211.96
Feed intake 591.609.11 632.4311.36
FCR 10.45 8.09
Intake and digestibility of nutrients in experimental goats
Attributes Group A Group B
Body wt.(Kg) 13.121.02 14.070.61
Body size (Kg) 6.880.39 7.260.24
Total dry matter intake
g/animal /day 568.0316.13 625.8154.43
g/per cent live weight 4.410.32 4.440.34
g/Kg
0.75
/day 83.544.94 86.026.71
Nutrient digestibility (%)
Dry matter 55.381.87 52.413.06
Organic matter 57.891.79 55.462.78
Crude protein 71.140.71 77.432.02
Ether extract 73.551.94 69.722.04
Total carbohydrate 62.582.53 57.463.17
Neutral detergent fibre 60.783.10 57.265.54
Acid detergent fibre 51.273.67 52.255.06
Cellulose 54.472.50 55.864.34
Hemi cellulose 59.606.36 53.131.91
CIRG

38
In another experiment Twelve male barbari goats
of 3-4 months of age were divided into two
groups (A and B) of six each as per completely
randomized design. Complete pellet feed
(control and treatment) were formulated. In
treatment pellet twenty five percent of
concentrate mixture was replaced with azolla
meal. Feeding and growth trial was conducted
for eight weeks. Group A was fed with control
pellet while group B was fed with treatment
pellet. The feed intake and body weight gain is
presented in table 1.The Average daily gain (g)
was 56.60 in group A and 78.12 in group B.
A metabolism trial of six days duration was
conducted on all the six animals of each group to
estimate the digestibility of different nutrients.
The dry matter and other nutrient digestibility
were similar in both the groups (Table 2). The
digestibility of crude protein was significantly
higher in group B (77.43) as compared to group
A (71.14).Nitrogen utilization in treatment group
of goats was better as compared to control group
of goats. Nitrogen intake (g/animal/day) was
statistically similar in group A (11.11) and group
B (12.36). Balance of nitrogen (g) was 4.14 in
group A while 6.40 in group B.

National initiative on climate resilient agriculture (nicra) on assessing resilience of
small ruminant production under changing climatic conditions in semi-arid zone
U.B.Chaudhary, Ashok Kumar, P. K. Rout and N.Ramachandran
An experiment was conducted on goats during
peak summer season for estimation of heat stress
and amelioration using herbal powder, liquid
anti stress and vitamin E. Results indicated that,
heat stress adversely affects body weight gain of
goats. Higher, concentration HSP 70 in the
plasma, in control than experimental group,
indicated effectiveness of different anti-stress
agents to combat the heat stress during peak
summer season. Herbal powder was found most
effective against heat stress than liquid form and
Vit-E. Results of another experiment conducted
to estimate the cold stress in goats and its
amelioration using herbal powder and Vitamin E
+ selenium combination, indicated positive effect
of herbal powder and Vit-E+Selenium against
cold stress in goats based on the Concentration of
HSP-27 & Ubiquitine Protein in plasma , as
lower units of these proteins were observed in
treatment groups than control. Herbal powder
was found most effective in reducing the cold
stress in goats than Vit-E+Selenium. Straw intake
was adversely affected by cold stress as lower
intake was recorded under control group. Effect
of feeding different quantity of concentrate (1.5
and 2.0% of body wt.) on stress conditions of
goats during winter season was also observed
and it was found that cold stress was not reduced
due to increase of the quantity of concentrate as
evidenced by the same concentration of
Ubiquitine Protein in the plasma of both the
group of goats.

Gene expression analysis in different tissues of
different goat breeds in response to heat stress
indicated, highly expression of Hsp70 protein in
kidney, liver and heart followed by brain, spleen,
lungs and testis. Kidney, liver and heart had two
fold higher protein expressions as compared to
other organ.
Farmers aware ness programmes with the aim to
create the awareness about climate change and
ameliorative measures to be taken were
organized at Dai, Raj asthan on 26.10.2013,
Rambha, Ganj am, Odisa on 23.11.2013 and at
Kendu Pali, Bargarh,Odisa on 28.2.2014. Each
camp was attended by > 100 goat farmers from
nearby districts. Lectures were delivered by PI;
Co-PI of the NICRA Proj ect and veterinary
officers of Animal Husbandry Department of
Raj asthan and Odisa state. Farmers were also
0
100
200
n
g
/
m
l
HSP 70 protein expression of liver & spleen in
liver
spleen
0
100
200
300
F
o
l
d

E
x
p
r
e
s
s
i
o
n
hsp 70 protein expression in organs
CIRG

39
given an opportunity to raise their queries
related to climate change and its impact on the
productivity of live stocks.
At the end, a questionnaire was distributed to t he
farmers with view to get a feedback with respect
of the gain of knowledge regarding climate
change and its probable effect on productivity of
livestock and agriculture. Most of the farmers
were not aware about the adverse effect of
climate change on the productivity of goats
however, awareness camp proved very useful to
cope up the adverse effect of climate change on
livestock productivity.
AICRP on improvement of feed resources and nutrient utilization in raising animal
production
U.B.Chaudahry, Ravindra Kumar, Ravi Ranjan
Goats are generally reared under extensive
system of feeding management with little or no
supplementation in the form of concentrate,
causing deficiency of energy protein and other
nutrients. The present experiment was
conducted to observe the effect of
supplementary feeding on growth potential of
goats under field conditions. Growing non-
descript goats maintained under extensive
system of feeding were divided in two groups;
control (Without supplementation) and
Experimental (With Concentrate
supplementation @1.5% of Bwt.) under present
experiment. Observation in terms of different
parameters related to production and rumen
fermentation were recorded. Significantly
higher body weights were recorded under
treatment group than control. Concentration of
VFA and ammonia nitrogen in the rumen was
also reported higher under treatment group.
The Blood picture indicated higher values of
hemoglobin and HCT under treatment group.
Units of glucose, urea nitrogen and total protein
in the plasma were also reported higher under
treatment group. A separate experiment was
conducted with the view that the supplementing
of Vit.E orally or injectable form to the male
goats may have a positive effect on improving
semen quality of goats. Adult male goats were
divided in two groups and were given traditional
ration (Control) and Traditional ration +vitamin-
E orally (Treatment group) observation in terms
of semen quality were collected form the goats
under both the groups. Same goats were
switched over to another experiment and
control group was given traditional ration
whereas experimental group was given
combination of Vit-E and Selenium in injectable
form. Observation in relation to semen
parameters was recorded. There was no
significant difference (P<0.05) in any parameters
estimated between control and treatment
group. However, AST values was significantly
higher (P<0.05) in control group (oral) compared
to treatment group of animals.

Effect of Vitamin E & Selenium on the semen quality of goats.
Parameters Control (Without vit.E+Selanium) Treatment (vitamin E & Selanium)
orally injectable Oral Injectable
ALT(IU/L) 22.267.28 26.587.28 18.9327.864 20.4818.614
AST(IU/L) 66.3808.550
26.5808.550
46.1779.235 20.48110.117
Testosteron(ng/ml) 10.5271.338 11.3491.338 11.1701.445 8.8951.583

Effect of oral supplementation of vitamin E given orally, on the semen quality of Goats
Attributes Control Experiment
Volume 0.6550.071 .6930.091
Mass Activity 0.3970.183 3.870.211
Motility 71.423.40 70.373.92
Live & Dead 73.501.81 64.164.62
Acrosome 65.231.99 59.804.89
HOS 66.921.83 61.005.14
CIRG

40

Net work programme on veterinary type culture (rumen microbes)
U.B.Chaudhary, Ravindra Kumar and V.K.Gupta
Following cultures of fiber degrading bacteria, isolated from rumen of goats were deposited to NIANP
Bangalore . The fiber degrading enzyme activities of these bacteria are as given below.

Name of the bacteria Isolate Enzyme activity ( mol glucose /minute)
CMCase Avicelase
Selenomonas sputiqana MSS-1 2.70 0.93
Enterococcus durans MED-7 2.34 1.82
Clostridium BS-6 1.83 1.57
Traceability, food safety standards and food chain evaluation (HACCP) pertaining to
goat meat and value added products
V. Rajkumar, Arun K. Verma and Khushyal Singh
Maj or Goat meat markets were identified in and
around this region. Goat meat processing units
were also identified. Out of the 45 export meat
plants approved by APEDA for slaughter of
animals 15 abattoirs have goat/sheep
slaughtering activity. Out of this 15, two abattoirs
are approved for stand-alone for sheep slaughter
and one is approved for exclusive slaughter of
Goat/sheep. Rest of the abattoirs has buffalo
slaughter activity also. Out of the 15 abattoirs
which have Goat/sheep slaughter activity, seven
are present in Uttar Pradesh. For computation
and final development of complete package of
practice we needed a computer modelling.
Perusal of references revealed that similar model
has been developed by the NRC on meat for
buffalo meat production. NRC model are
modified to suit our requirement .Under this
proj ect and within the time mentioned, following
studies had been conducted. Under the work
Food safety and HACCP standards for goat
meat and products production process
Rosenthal Meat Science and Technology center,
USA, HACCP plan was used as the base model
for the development of our HACCP models.
Already prepared goat /sheep slaughter plan
was prepared in the above said plan that suits
our laboratory and Indian conditions.
The other two plans prepared and presented are
fully cooked, not shelf-stable product HACCP
plan for Nuggets and sausages and heat treated
(Annexure I), shelf-stable product HACCP plan
for Murukku and Nimkee (Annexure II). In the
half yearly IRC we have presented the data
related to the nuggets/sausages and the next plan
we have added in the next six months. HACCP
plan document contains he signature page and
the plan summery in the tabular form. Plan
summary deals with the Critical Control Points
(CCP) in the preparation of the products. There
are three important critical points for nuggets
/sausages and for Murukku/Nimkee. Product
flow chart also presented and the product hazard
analysis table also prepared the HACCP plan.
Description of CCP, their monitoring procedure
also prepared and presented. Verification and
record keeping procedure for all the CCP has
been identified and the individual records to be
maintained are narrated and presented in the
tabular form.
HACCP data for Goat meat products
Mostly microbial analysis was carried out.
Residue analysis will be done when the facilities
are created and accordingly we will modify
critical limits for the chemical and other related
residues. To identify critical control points
microbiological quality of the stages of nuggets
processing including slaughter has been done
(Table 1).
CIRG

41
Table 1. Physico-chemical and microbial traits of freshly prepared nuggets and snack meat
products.
Traits Nuggets Murukku/Nimkee
pH 6.14 6.01/6.03
Water Activity 0.976 0.421/0.65
W-B Shear force values (kgcm
-2
) 1.38 TPA analysed
Moisture, % 59.46 6.32/1.32
Fat, % 17.08 21.53/28.32
Standard Plate counts 4.21 1.04/1.26
Psychrotrophic bacteria counts 3.43 Nil/Nil
Table 2. Standard plate counts at various stages of Nugget/sausage and Nimkee/Murukku
processing including slaughter
Place of sampling SPC (log CFU 10
-1
sqcm)
Slaughter house floor 5.01
Slaughter house wall 3.87
Bleeding knife (Iron) 3.27
Dressing knife (Iron) 3.46
Butchers hand 3.90
Carcass splitting chopper 4.01
Deboning knife I (Stainless steel) 2.74
Deboning knife II (Stainless steel) 2.63
Carcass surfaces
a. Carcass neck portion 4.64
b. Carcass Loin cut 4.87
c. Carcass Leg cut 3.43
Carcass cutting wood 3.61
Deboning table 3.81
Surface of meat mincer 2.01
Surface of bowl chopper 1.02
Surface of SS Emulsion box 2.18
Surface of nuggets cutting plates 1.71
Surface of the murukku/nimkee making machine 1.09
Plates used for making Nil
Wooden frame and handle used Nil

Table 3. Estimation of bacterial counts in the raw materials used in the formulation of goat meat
nuggets/sausage and Murukku/Nimkee
S.No. Raw materials SPC (log CFU g
-1
)
1. Meat Keema 3.92
2. Meat fat 4.06
3. Casings 3.72
4. Maida 3.07
5. Meat powder 1.86
6. Murukku/nimkee powder 1.09
7. Oil Nil
8. Spices 2.04
9. Condiments 1.03

CIRG

42
Similarly estimation of bacterial counts in the
raw materials used in the formulation of Nugget
has been done. After the complete analysis
HACCP design will be prepared. Perusal of
Table 7 reveals that slaughter house floor (log
5.01), wall (log 3.87), butchers hand (log 3.90),
bleeding knife (log 3.46) and carcass splitting
chopper (log 3.95) had higher SPC. This point
needs consideration. Regarding raw material like
keema (log 4.41) and fat (log 3.93), if the initial
counts can be reduced, fresh product count can
also be reduced considerably.

Value chain for the development of goat products with healthy traits
A K Verma and V Rajkumar
Development of healthier chevon nuggets: P/S
ratio standardization by the blend of animal
fat and vegetable oil
Attempt was made to develop healthier chevon
nuggets through standardization of PUFA/SFA
ratio in the range of recommended value via
replacement of animal fat by vegetable oil.
Chevon nuggets were processed through
replacement of goat fat to 0%, 33.33%, 50% and
100% with vegetable oil of known fatty acid
profile and their quality characteristics were
determined. Cooking yield of chevon nuggets
prepared with 50% and 100% replacement of
goat fat was significantly higher and product
with 100% goat fat had low yield. Ash contents
in meat emulsion and product with 100%
vegetable oil was lower than other treatments.
Replacement of goat fat with vegetable oil
significantly affected the Hunter colour lightness
values.
Table 1: Effect of goat fat replacement with vegetable oil on physicochemical characteristics of goat
meat nuggets (n=6)
Parameters GF GFVO11 GFVO21 VO
Emulsion pH 6.420.01 6.400.01 6.430.01 6.410.01
Nuggets pH 6.480.01
ab
6.460.01
b
6.490.01
a
6.470.01
ab

Emulsion stability (%) 91.660.42
b
92.730.21
b
92.100.54
b
97.000.21
a

Cooking yield (%) 90.500.28
c
95.170.39
a
94.000.49
b
96.170.25
a

Emulsion moisture (%) 67.480.41 67.160.46 67.540.40 66.860.16
Emulsion fat (%) 8.520.33 8.430.35 8.480.24 8.320.37
Emulsion protein (%) 13.080.06 13.120.17 12.980.15 12.930.19
Emulsion ash (%) 2.780.01
a
2.750.01
a
2.710.05
ab
2.650.03
b

Nuggets moisture (%) 65.350.30 65.530.27 65.130.37 65.360.37
Nuggets fat (%) 8.560.15 9.360.38 9.290.22 9.100.22
Nuggets protein (%) 14.690.20 14.790.18 14.260.26 14.560.11
Nuggets ash (%) 2.800.05
a
2.820.02
a
2.720.08
ab
2.590.01
b

Table 2: Effect of goat fat replacement with vegetable oil on various categories of fatty acids (% of
total fatty acid) in goat meat nuggets (n=6)
Fatty acid GF GFVO11 GFVO21 VO
SFA 50.191.82
a
35.090.65
c
40.821.62
b
23.970.71
d

MUFA 41.601.57
a
34.611.17b
c
38.441.15
ab
31.641.84
c

PUFA 7.890.39
d
31.900.37
b
20.471.11
c
43.731.26
a

n3 PUFA 1.310.09
b
2.120.05
a
2.810.46
a
2.830.22
a

n6 PUFA 6.140.45
d
29.550.40
b
16.101.05
c
38.380.83
a

PUFA/SFA 0.160.01
d
0.910.03
b
0.520.05
c
1.830.05
a

N6/N3 PUFA 4.870.58
b
13.970.49
a
6.701.24
b
14.001.18
a

Increase in proportion of vegetable oil in chevon
nuggets significantly increased yellowness
value. Textural properties of the product did not
affect significantly due to goat fat replacement,
however product with 100% vegetable oil
required lowest shear force value and work of
shear. Sensory attributes of the products were
significantly affected due to goat fat replacement,
except appearance and chevon nuggets with
33.33 % and 50 % replaced goat fat received
CIRG

43
highest acceptability scores. Higher proportion
of vegetable oil in product significantly
improved PUFA particularly omega-6 and
decreased saturated and monounsaturated fatty
acids. Product with 33.33 % and 50 % replaced
goat fat showed P/S ratio (0.52-0.91) in the range
of recommended value. Thus partial replacement
of goat fat with vegetable oil can provide
healthier chevon nuggets.
Effect of replacing goat fat with vegetable oils
on the quality characteristics and fatty acid
profile of chevon nuggets
Standardization of chevon nuggets having
healthier fatty acid conformation such as
PUFA/SFA ratio and omega-6/omega-3 fatty
ratio was attempted. Two sources of fat from
plant origin such as vegetable oil (VO) and
linseed oil (LSO) were selected and were used to
replace 50% and 100% goat fat (GF) in chevon
nuggets. Four types of products were prepared
such as control nuggets (7% goat fat), GFLSO
nuggets (3.5% goat fat + 3.5% LSO), GFVOLSO
nuggets (3.5% goat fat + 3.5% combination of VO
and LSO) and LSO nuggets (7% LSO) and their
different quality characteristics were evaluated.
Batter stability and cooking yield of control
nuggets and LSO nuggets were significantly
lower as compared to other two products. Fat
contents in control emulsion and nuggets were
significantly lower. LSO emulsion and nuggets
had significantly lower ash contents. Hunter
colour lightness value for control nuggets was
significantly higher while LSO nuggets had
higher redness and yellowness values than other
products. Texture profile analysis values for LSO
nuggets were significantly lower in relation to
other products. Sensory evaluation revealed that
appearance and flavour scores for control
nuggets were significantly lower, however
overall acceptability scores of all the products
did not differ significantly. Fatty acid profile of
GFVOLSO nuggets had the most desired fatty
acid conformation with PUFA/SFA ratio as 0.85
and omega-6/omega-3 fatty acid ratio as 1.40.
Thus 50% replacement of goat fat with
combination of vegetable oils can impr ove
functional characteristics of chevon nuggets,
which can be healthier also.
Table 3: Effect of goat fat replacement with two vegetable oils on physicochemical characteristics
of goat meat nuggets (n=6)
Parameters GF GFLSO GFVOLSO LSO
Emul si on pH 6.420.01
a
6.350.01
b
6.340.02
b
6.360.01
b

Product pH 6.480.01
a
6.410.01
c
6.410.01
c
6.430.01
b

Batter stabi l ity (%) 91.660.42
c
96.190.09
a
96.690.33
a
94.180.16
b

Product yi el d (%) 90.500.28
c
97.830.25
a
97.940.16
a
97.070.10
b

Emul si on moi sture (%) 67.480.41 66.730.23 67.250.13 67.330.14
Emul si on fat (%) 8.520.15
b
10.160.35
a
10.270.27
a
10.730.21
a

Emul si on protei n (%) 13.160.07 12.710.21 12.910.21 12.910.19
Emul si on ash (%) 2.780.01
a
2.780.03
a
2.730.02
a
2.330.04
b

Product moi sture (%) 65.350.30 65.870.57 66.150.44 65.880.15
Product fat (%) 8.560.15
c
10.080.21
b
10.660.19
ab
11.010.61
a

Product protei n (%) 14.780.19 14.350.07 14.560.15 14.510.14
Product ash (%) 2.670.05
a
2.750.03
a
2.660.06
a
2.500.04
b

Table 4: Effect of goat fat replacement with two vegetable oils on various categories of fatty acids
(% of total fatty acid) of goat meat nuggets (n=6)
Fatty acid GF GFLSO GFVOLSO LSO
SFA 50.191.82
a
43.291.01
b
37.210.46
c
24.420.54
d

MUFA 41.601.57
a
31.831.15
b
30.880.89
bc
27.770.24
c

PUFA 7.890.39
c
30.470.67
b
31.600.58
b
47.590.42
a

n3 PUFA 1.310.09
d
23.150.69
b
13.090.25
c
34.140.50
a

n6 PUFA 6.140.45
d
8.950.12
c
18.260.33
a
13.360.32
b

PUFA/SFA 0.160.01
c
0.770.03
b
0.850.01
b
1.960.06
a

N6/N3 PUFA 4.870.58
a
0.420.01
c
1.400.01
b
0.390.01
c

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44

Screening of ingredients for development of
low salt meat products
Various ingredients low in sodium content were
identified for the development of non-emulsion
based meat product. These ingredients were
used as a substitute of sodium chloride (common
salt) to develop the meat product. Preliminary
trial for the development of low sodium goat
meat pickle was conducted and evaluated
organoleptically. Additionally physicochemical
characteristics and fatty acid profile of control
goat meat pickle was evaluated. Goat meat pickle
was found to be rich in animal protein (19.50%).
Fatty acid profiling of the pickle revealed that the
product contained high amount of healthier fatty
acids such as linoleic acid (18.23%), alpha
linolenic acid (16.83%) and arachidonic acid
(6.17%). The ratio of omega-6/omega-3 fatty acids
(1.09) in the product was found to be in the range
of recommended value (1-4).

Table 5: Categories of fatty acids (% of total fatty acid), P/S and omega-6/omega-3 fatty acid ratio in
goat meat pickle
Fatty acids Value
MCT 0.19
SFA 20.80
MUFA 34.62
PUFA 43.33
n-3 PUFA 18.11
n-6 PUFA 19.68
PUFA/SFA 2.09
n-6/n-3 PUFA 1.09

Effect of age and management practices on quality characteristics of Barbari goat meat
Physicochemical, colour and fatty acid profile of
Barbari goat meat from four groups viz., 12
month intact, 12 month castrated, 15 month
intact and 15 month castrated were compared.
Moisture content in meat was significantly
decreased in castrated goat and more significant
effect was observed with age while fat content
increased. Protein and ash contents were
significantly higher in meat from older animals.
Water holding capacity (WHC) was found
significantly higher in meat from older animals
from both groups. Water soluble proteins (WSP)
were significantly high in meat from 15 month
castrated goat while salt soluble proteins (SSP)
and total soluble proteins (TSP) were high in
younger intact animals. Hunter colour
evaluation of goat meat showed that meat from
castrated goats was lighter while meat from
younger animals was redder. Palmitic acid
(C16:0) content was increased with age and it
was significantly higher in 15 month castrated
goat meat. Percent stearic acid (C18:0) was
significantly decreased with age and it was much
lower in castrated animal. Oleic acid (C18:1)
content was significantly higher in castrated
animals while amount of linoleic acid (C18:2)
was higher in intact animals and both the fatty
acids increased with animal age. Total saturated
fatty acids (SFA) were high in meat from younger
animals. Amount of monounsaturated fatty acids
(MUFA) were found significantly higher in meat
from castrated animals while meat from intact
animals had higher polyunsaturated fatty acids
(PUFA). Meat from intact animal had
significantly higher total omega-3 as well as
omega-6 fatty acids.

CIRG

45

Goat Health Division
Patho-epidemiological studies on emerging and existing diseases of goats.
R.V.S. Pawaiya, S.V. Singh, D.K. Sharma, Ashok Kumar, V.K. Gupta, Naveen Kumar, K. Gururaj,
Shivasharnappa N., A.K. Mishra, Nitika Sharma, Souvik Paul, H.A. Tiwari and V.K. Chaturvedi

A total of 3883 biological samples collected/
received from from Chattisgarh, J&K,
Maharashtra, M.P., Raj asthan, U.P. which
included sera, blood, swabs, feces, tissues and
others. The samples were subj ected to various
laboratory tests for identification of diseases and
the results are given in table below.
Laboratory test results
State JD Brucellosis CAE*
Sera Feces
CIRG - - Goat: 10/48 = 20.83%;
Sheep: 22/47 = 46.80%
-
J & K - - 47/335 = 14.02% -
Maharashtra 157/1107 = 14.1% 199/1080 = 18.4% 1/82 = 1.2% -
M. P. 57/119 = 47.8% 13/39 = 33.3% 17/202 = 8.4% -
Rajasthan 13/16 = 81.2% 1/5 = 20.0% 0/12 = 0.0% 7/45 = 15.55%
U. P. 15/71 = 21.1% 12/25 = 48.0% 20/204 = 9.8% 6/45 = 13.33%
Total 242/1313 = 18.43% 225/1149 = 18.43% 117/930 = 12.58% 13/90 = 14.44%
*A total of 90 sera samples of goats from Rajasthan and Uttar Pradesh (Mathura Distt. & adjoining areas) were screened for
presence of caprine arthritis-encephalitis (CAE) antibodies employing competitive ELISA test (VMRD, USA). Of these, 13
(14.44%) were found to be seropositive [4 (4.44%) strong positive & 9 (10%) weak positive) for CAE.

Out-patients attended
A total of 113 clinical cases comprising of
Endoparasitism (30), Ectoparasitism (25),
Acidosis (11), Diarrhoea (10), Dog bite (10), Bloat
(8), Pregnancy toxaemia (6), Udder impetigo (5),
Caesarean section (2), Urolithiasis (1), Plant
poisoning (1), Hypovitaminosis A (1),
Polioencephalomalacia (1) were diagnosed and
treated.
Morbidity
A total 5474 animals were treated at CIRG farms
for various disease conditions including
diarrhea/enteritis (67.63%), dullness/PUO
(8.88%), wound/abscess (5.52%), lameness (4.30),
pneumonia/ cold (3.20%), general weakness
(2.21%), tympany/colic (1.20%), swollen udder/
mastitis (1.13%), abortion (1.11%) etc.
Mortality
A total of 229 necropsies were conducted from
1.4.2013 to 31.3.2014 involving 59 (25.76%)
animals from Animal Health Shed, 53 (23.14%)
from Jamunapari unit, 37 (16.15%) from Barbari
unit, 33 (14.41%) from Sheep unit, 18 (7.86%)
from Jakhrana unit, 15 (6.55%) from NFR&PT
and 14 (6.11%) from PRSM. The maj or causes of
death diagnosed were enteritis (17.46%),
pneumonia (17.03%), septicaemia (6.98%),
hemonchosis (4.37%), anemia/weakness &
pregnancy toxemia (3.05% each), gastro-enteritis
(2.18%), toxaemia (2.18%), pneumo-enteritis,
acidosis, predation, autolysis (14.41%) and
miscellaneous diseases (14.84%).
Disease investigation
Eighty samples were processed for
histopathological studies. Histopathological
diagnosis revealed cases of granulomatous
enteritis, acute serous pneumonia, suppurative
pneumonia, bronchopneumonia, mycotic
pneumonia, bronchioloalveolar proliferative
changes etc. Disease investigations were carried
out at three villages in Mathura district and one
at Garh Mukteshwar in U.P. and arthritis, FMD
and suppurative lymphadenitis were diagnosed
in several goats. Parasitologically, out of total 542
fecal samples, 139 were positive for coccidian
oocysts, 74 for nematode ova and 6 for tapeworm
eggs. More than 60 samples from goats and sheep
were collected and processed for bacteriological
isolation.The samples comprised of blood, pus,
CIRG

46
milk, lung, liver, pleural fluid, nasal discharge,
kidney, skin scrapings etc. The bacterial
pathogens isolated and identified were
Staphylococcus aureus, Staphylococci,
Streptococcus, Bacillus, E. coli, Pasteurella
multocida and Pseudomonas aeruginosa.
Fusobacterium necrophorum was identified by
PCR from foot rot affected goats in Bundi
District, Raj asthan.PCR amplification and
sequencing of E. coli, Staphylococcus aureus
from diarrhoea and pneumonia in goats.

Effect of nutritional deficiency diseases on gene expression profiles in goats.
R.V.S. Pawaiya, U.B. Chaudhary, Nitika Sharma, Shivasharnappa N. and S.P. Singh
Low Cu and Zn feed formulation was carried out as per the following table, considering the fact that
normal requirement levels for Cu at 7-12 mg/kg DM and Zn at >35 mg/kg in ruminants.

Sl. No. Ingredients Quantity (g/kg)
1. Maize 542.50
2. Starch 200.00
3. Casein 230.00
4. Mineral mixture 20.00
5. Vitamin premix 5.00
6. Sodium bicarbonate 2.50
7. Cu = 4.54 mg/kg

Zn = 21.25 mg/kg

Experimentation was carried out in 36 6-9 month
old male barbari goats after dividing them in 4
groups with 9 animals in each and treatment was
given as: Group-A: Copper-deficient diet;
Group-B: Zinc-deficient diet, Group-C: Copper &
Zinc combined-deficient diet; and Group-D:
Control with balanced ration. Regular
observation on body wt. revealed consistent
increase upto 180 weeks, with group-B animals
showing marginally better body wt gains over
time. Testicular measurements of experimental
animals did not show significant changes among
groups. The correlation of testicle sizes was quite
significantly associated with the body wt.,
especially of testicular circumference and girth of
left testicle. Effect of Cu & Zn deficient diet
feeding on dry matter intake (g/d/animal) was
found to be 640.418.43 in Gr-A, 607.228.00 in
Gr-B, 643.758.48 in Gr-C and 636.528.38 in Gr-
D.
Clinically, the animals started showing signs,
especially of Cu-deficiency in the A group, from
60 days onwards with progressive roughness of
hair coat and increasing tendency of coarseness
of hairs till 165 days as depicted in the figures
below. Group-C animals fed Cu & Zn combined
deficient diet also shoed tendency of rough hair
coat with increasing time however, the degree of
changes were less intense compared to the
group-A animals. Group-B (Zn-deficient) and
control (group-D) animals did not show any
discernible changes in their skin hair coat.
Pathologically, gross lesions were not observed
in an animal from Cu-deficient group-A which
died at about 90 days of experimentation.
However, the animal from Zn-deficient group-B
died at about 105 days showed significantly
atrophic testes in comparison to the control
animal of group-D that was also died on the same
day. The overall size and weight of both the
testes of Zn-deficient animal was significantly
decreased (wt. 9.19 and 9.39 g/ length 5.0 and 5.5
cm for right and left testicles, respectively) in
comparison to the control animal (wt. 26.27 and
27.74 g/ length 6.5 and 7.2 cm for right and left
testicles, respectively).
Genetic resistance study in indian goats against gastrointestinal nematode,
Haemonchus contortus infection
D.K. Sharma , Souik Paul, Naveen Kumar, P.K. Rout and V.K. Gupta
CIRG

A total of 1203 faecal (749 from Jamunapari and
454 from Jakhrana) were collected and examined
for gastrointestinal nematodes infection specially
for Haemonchus contortus. The incidence of
different parasitic infections was reported. The
highest coccidian incidence was recorded 100 per
cent in Barbari. The highest strongyles incidence
(predominantly Haemonchus contortus) was 46
per cent again in Barbari. The other parasitic
infections recorded were Moniezial and
Strongyloides spp. The data generated on FEC
was transformed through log transformation to
normalise the skewedness. The transformed data
was used for statistical analysis. The data was
analysed using least squares means analysis
model I (Harvey, 1990) based on sire line. Non
genetic factors like age, sex, type of birth, birth
weight and season of collection along with
genetic effect of sire were considered in analysis.

Farms Age Observations Incidence
Cocci + Strongyle+ Moniezia+ Strongyloides+
Barbari Total 393 74.3(292) 38.7(152) 5.08(20) 0.50(2)
Adult 220 65.0(143) 46.3(102) 0.45(1) 0.90(2)
0-3 M 30 90.0(27) 3.33(1) 16.6(5) 0.0
>3-6M 73 69.8(51) 23.28(17) 4.1(3) -do--
>6-12M 70 100.0(70) 35.7(25) 15.7(11) -do--
Jamunapari Total 749 83.2(623) 18.8(141) 2.00(15) 3.33(25)
Adult 110 85.5(94) 41.8(46) 3.63(4) 15.45(17)
0-3M 33 93.9(31) 0.0
>3-6M 5.7 80.8(464) 12.6(64) 2.16(11) -do--
6-12M 99 92.0(92) 13.1(13) 8.08(8)
Jakhrana Total 454 81.9(372) 34.6(157) 5.28(24) 0.66(3)
Adult 191 82.2(157) 27.2(52) 3.66(7) 1.57(3)
0-3M 51 68.6(35) 5.88(3) 1.96(1) 0.0
>3-6M 89 98.8(88) 71.9(64) 12.35(11) -do--
>6-12M 123 80.5(99) 16.26(20) 4.06(5) -do--

Most Resistant and susceptible Sires in Jakhrana and Jamunapari flocks at CIRG
S.No. Jakhrana Jamunapari
Sire LFEC GSM( x200) Sire LFEC GSM( x200)
Resistant Resistant
1 201(28) 5.030 152 6659(5) 4.395 81
2 0 (3) 5.039 154 6681(7) 4.505 91
3 598(2) 5.076 160 5902(10) 4.515 91
4 154(6) 5.147 171 6546(5) 4.537 93
5 190(38) 5.154 173 6996(11) 4.633 103
Susceptible Susceptible
1 152(4) 5.947 382 5012(8) 6.105 448
2 12(3) 5.907 367 6697(5) 5.991 400
3 672(6) 5.681 293 5255(5) 5.321 205
4 314(6) 5.657 286 4682(7) 5.313 203
5 588(4) 5.377 216 5277(5) 5.296 200

A total of 14 sires with at least 2 offspring were
evaluated. For Jamunapari, total 57 sires with at
least 3 offspring were considered for evaluation.
The effect of sire in both the breeds was not
significant; however, the sires in both breeds
were listed and graded on the basis of mean
LFEC. The five resistant and five susceptible sires
in both breeds are presented in Table above. Non
CIRG

48
genetic factors like season of collection and age
were found to have significant effect on faecal
egg count in infected animals in both the breeds
of goats (Table). On the other hand, effect of
factors like type of birth, birth weight and sex
was not significant.
A total of 120 blood samples (Jamunapari) were
collected and haematological values were
ascertained for generating the data for genetic
correlation. The molecular techniques of
extraction of RNA from tissues of natural
infected (H. contortus) and cDNA preparation
were standardized.
Factor wise Least Squares Means Faecal Egg Count in Haemonchus contortus infection (Natural) in
Jakhrana goats.
Source of variation No. of Observation Least Squares means SE
Age
0-3M 32 4.612 0.236
>3-6M 73 4.995 0.187
>6-12M 11 5.753 0.332
>12M(Adult) 85 5.976 0.208
Season of Collection
Summer 71 5.381 0.198
Rainy 68 5.754 0.215
Winter 62 4.868 0.226
Sex
Male 94 5.302 0.196
Female 107 5.366 0.191
Type of Birth
Single 66 5.341 0.163
Twin 127 5.530 0.159
Triplets 8 5.132 0.378
Birth Weight (Kg)
<2.60 10 4.801 0.344
>2.6-3.0 72 5.365 0.184
>3.0-3.5 99 5.376 0.179
>3.5 12 5.742 0.306
Factor wise Least Squares Means Faecal Egg Count in Haemonchus contortus infection (Natural) in
Jamunapari goats
Source of variation No. of
Observation
Least Squares
means
SE
Age
0-3M 40 4.367 0.147
>3-6M 393 4.967 0.077
>6-12M 105 5.211 0.104
>12M( Adult) 340 5.291 0.075
Season of Collection
Summer 312 4.559 0.720
Rainy 264 5.152 0.814
Winter 302 5.167 0.667
Sex
Male 280 4.897 0.071
Female 598 5.021 0.058

CIRG

49
Achieving improved livelihood security through resource conservation and diversified
farming system approach in Mewat
D.K. Sharma and P.K. Rout
A total of 7 animal health camps were organized
in adopted villages to provide door to door
health services to animal owners. In 4 Villages i.e.
Singalhedi, Jharpedi, Badarpur and Maroda, 84
goats were vaccinated for FMD and HS, 103 goats
were vaccinated for ET and 90 goats were
vaccinated for PPR. A total of 87 animals (goats,
sheep and buffaloes) were treated for various
ailments in camps. In all 257 goats were
dewormed and 54 dipping were performed in
camps. Recording of data on production like
milk, body weight and reproductive
performance was recorded. Under reproductive
performance kidding rate in Sirohi (Jharpadi),
Jakhrana and Sirohi (Maroda) was 1.16, 1.40 and
1.21 litter/kidding, respectively. The overall
mortality recorded in 2013-14 was 2.51 per cent.
With highest sold percentage of goats of 45.05,
being recorded in Jharpadi, the income of
farmers from goats was enhanced with
Rs.15400/annum. During upgrading programme
of local goats, the performance of new genotype
Sirohi x Totapari goats was recorded and
compared with the Sirohi animals. The new
genotype performed remarkably well in local
conditions.
Population growth of goats in adopted villages
Villages Opening
Balance
Breed
able
Female
Addition Total Deaths Sold Available
Jharpadi 56 30 35 91 1 41 49
Singhalhedi 54 22 31 85 4 37 44
Maroda 40 19 23 63 1 26 36
Performance of Sirohi and Sirohi x Totapari cross
Crosses Birth
Wt. (Kg.)
15 Day
Wt. (Kg.)
1 M
Wt. (Kg.)
3 M
Wt. (Kg.)
Sirohi x
Totapari

Male 2.83(4) 5.95(4) 8.13(4) 17.0(2)
Female 3.01(6) 5.80(2) 7.86(3) 14.5(4)
Totapari x
Sirohi

Male 3.54(4) 5.90(3) 7.23(3) 12.6(2)
Female 3.10(3) 5.80(3) 6.95(2) 11.8(2)
Sirohi x Sirohi

Male 2.62(15) 4.93(12) 6.80(8) 12.51(11)
Female 2.53(13) 5.14(11) 6.19(11) 12.4(9)
Development of herbal anthelmintic and acaricidal formulations for goats
Ashok Kumar, D.K. Sharma, Nitika Sharma, V.K. Gupta, U.B. Chaudhary, H.A. Tiwari and Vinay
Chaturvedi
Collection and extract preparation of plant
material
Selected 10 plants were collected from herbal
garden maintained at CIRG , Makhdoom and
near by area and coded as CIRG-1 to CIRG-10.
Crude extract was prepared by using Methanolin
Soxhlet and microwave extraction system. The
percentage yield was ranged from 7-27%.
Brine Shrimp Lethality Assay: Artemia salina
Leach cysts were obtained from pet USA. About
5 mg of cysts is incubated in plastic bottle with 50
ml of artificial sea water (ASW) of pH 8 at 28 C
during 48 hours. The nauplii slowly move out of
the vial through the perforated lid into the beaker
which is pipetted out using a micropipette. The
shrimp of 20-32 hours old were pipetted in 24-
CIRG

50
multiwell plates (20 per well) containing 1 ml of
each plant crude extract. Concent rations of
extracts (10, 5, 2.5, and 1.25 mg/ml), and
Dichromate Potassium (K2Cr2O7) solution (1.0,
0.5, 0.25 and 0.125%, used as positive control)
were diluted in artificial sea water and were
added to the wells (1 ml per well). Tree replicates
were run per concentration. They are then
incubated at temperature of 25C. To determine
the acute LC50, the number of death nauplii in
counted in every well after 6 h. counting for the
chronic LC50 begins 24 h after initiation of the test.
These data was processed in a readily available
personal computer program (Finney) to estimate
LC50 values for statistically significant
comparison of potencies.
Artemia salina lethal concentration fifty (LC50)
Plant
Extracts
LC50 current
toxicity at 6h
(mg/ml)
LC50 chronic toxicity at 24h
(mg/ml)
CIRG-7 7.50 5.02
CIRG-8 5.53 1.46
CIRG-10 13.0 1.95
CIRG-9 2.50 1.26

Adult worms mortality test: Adult worms were
collected from goats in Slaughterhouse of Agra
city. Immediately after slaughtering, the
abomasum was removed, opened and placed in
37 C saline (0.9%). The mobile worms were
rapidly collected and put into 24-multiwell
plates, 10 worms per well in 2 ml of each plant
extract solution at 37 C in saline at varying
concentrations (50, 25, 12.5 and 6.25 mg/ml) or
Albendazole solution (1.0, 0.5, 0.25, 0.125 %, used
as positive control). The lowest LC50 was
recorded 22.71 ( 2 hrs), 37.2 (3 hrs), 21.76 (6 hrs) ,
27.06 (6 hrs), 30.33(6hrs) and 34.32mg/ml (3hrs)
interval in CIRG-2, CIRG-5, CIRG-3, CIRG-6,
CIRG-1and CIRG-4 respectively. The results
indicated that 2 exerts pronounced anthelmintic
effect against adult worms of Haemonchus
contortus with more than 50% mortality at a dose
of 25 mg/ml from the sixth hour of incubation.
This action is equivalent to 1% albendazole. The
generalized death of all the worms intervened
between 6 and 24 hours.

Adult worms Lethal concentration fifty (LC50) mg/ml
Extracts plant 1 h 2 h 3 h 6 h
CIRG-2 48.62 22.71 15.32 -
CIRG-5 - 66.34 37.2 -
CIRG-3 50.81 39.68 29.15 21.76
CIRG-6 105.61 35.90 28.95 27.06
CIRG-1 - - 61.61 30.44
CIRG-4 65.30 47.86 34.32 -
CIRG-7 - 106.0 - -
CIRG-8 102 - - 40
CIRG-10 - - - 88
CIRG-9 - - - 448
Mortality test of third-stage infective larvae
Fecal samples were collected from the goats
reared at CIRG and farmers goat flock nearby to
Institute for Haemonchus contortus larvae culture.
H. contortus L3 were obtained by fecal culture.
Eggs reached the L3 stage after 8 days. The L3
were then collected by sedimentation using
Baermanns devices. The larvae suspension with
a concentration of 200 larvae/ml, was distributed
in 24-multiwell plates (0.5 ml per well).
Concentrations of crude extracts (100, 50, 25 and
12.5 mg/ml), and Albendazole solution (2.0, 1.0,
0.5, and 0.25%, used as positive control) were
diluted in distilled water and were added to the
CIRG

51
wells (0.5 ml per well). In addition, negative
controls in distilled water was also included in
the assay. In first phase , Anthelminitic activity
on larvae of haemonchus contortus infection was
good with CIRG-2, CIRG-5, CIRG-3, CIRG-6, and
CIRG-4 showing lowest LC50 of 8.44,14.27
,11.89,3.44 and 10.62 mg/ml respectively in 1-6
hrs interval .The action of plant extracts of L3
larvae indicates that they are all effective against
the larvae of the parasite with 50-68 % mortality
at 6 h at a dose of 25 mg/ml, which was better in
comparison with 1% albendazole at the same
time. Again CIRG-8 seems to have the best
efficacy as well as CIRG-9. The precocity effect is
detained by CIRG-9 that induced 20% mortality
of larvae in the first hour of incubation.
Larvae L3 lethal concentration fifty (LC50) (mg/ml)
Extracts 2 h 3 h 4 h 5 h 6 h 24 h
CIRG-2 31.39 20.97 13.14 10.69 8.44
CIRG-5 25.90 19.25 14.27 - -
CIRG-3 33.53 17.59 11.89 - -
CIRG-6 11.30 7.95 3.44 - -
CIRG-1 65.31 47.86 34.25 - -
CIRG-4 - 17.14 13.32 10.61 -
CIRG-7 - - - - 16.02 10.94
CIRG-8 - - - - 8.57 8.57
CIRG-10 - - - - 16.16 9.14
CIRG-9 - - - - 11.33 8.01
Chromatographic seperation of CIRG-2 leaves
MeOH extract
Five chromatographic fractions were obtained
during column chromatography of CIRG-2
leaves MeOH extract. Fraction 2 and 4 were used
for anthelmintic activity since they are in moj or
proportion while rest of the fractions were in
very minute quantity. The LC50 calculated for
fraction 2 at 1, 2, 3 and 6 hour post exposure.
Mortality percent recorded were 44.4, 37, 37.4,
62.7, 70.8; 77.8, 58.9, 60, 84.1, 84.7 and 100, 100,
100, 100, 100 post exposure at concentration
3.125, 6.25, 12.5, 25 and 50 mg/ml at different time
intervals. LC50 calculated was 11.9 and 3.66
mg/ml at 2 and 3 hours post exposure
respectively.
Egg Hatch assay
Fecal samples collections were made both at
CIRG and at Farah and Mathura for Haemonchus
contortus eggs extraction. Eggs were extracted
from feces. The egg suspension with a
concentration of 200 eggs/ml, was distributed in
24-multiwell plates (0.5 ml per well).
Concentrations of crude extracts (100, 50, 25, and
12.5 mg/ml), and Albendazole solution (2.0, 1.0,
0.5, and 0.25%, used as positive control) were
diluted in distilled water .The LC 50 recorded
were 7.06,5.8, 40.7,47.0,21.7 and 81.7 mg/ml in
CIRG 3,2,1,5,4,6 respectively.
Phytochemical screening of plants and results:
both qualitative and GC-MS analysis was done.
Thin Layer Chromatography of plant extrcats :
The TCL Plates (Silica gel 60 F 254 nm, MERCK)
was used. The solvent system was kept n-
Hexane:Acetone(75:35). The plates were
examined under UV 366 and UV 254 nm and Rf
values were recorded.
Rf values of various spots detected under 366 nm on TLC plate (Sovent front= 71 mm). Sovent
system used was
CIRG-3 CIRG-4 CIRG-1 CIRG-2 CIRG-6
0.09 0.09 0.029 0.10 0.16
0.70 0.57 0.51 0.53 0.50
0.77 0.77 0.77 0.74 0.61
0.86 0.86 0.89 0.89 0.77
0.86
CIRG

52
Clinical trial of plants Extracts : CIRG-3 and
CIRG-2 was given at The doses of 100 mg/kg
body weight o.i.d for 3 days as oral formulation
. In CIRG-3 ,the percentage faecal egg count
reduction was 11.81% and 50.26% 7
th
and 14
th
day
post treatment . The EPG counts also showed
significant reduction on 7
th
day post treatment
and on day 14 post treatment. Similarly, The
CIRG-2 crude methanolic extract at same dose
rate, resulted percentage faecal egg count
reduction of 16.42 2.63 and 59.202.26 on the 7
th

and 14
th
day post treatment respectively. The
EPG counts also showed significant reduction on
7
th
and 14 post treatments. In positive control, six
animals were treated with tab. Albendazole @ 7.5
mg/kg body weight single dose on Day 0 and
Day 14. The percentage faecal egg count
reduction was recorded as 96.027 1.95 and
100.00 0.00 on the 7
th
and 14
th
day post
treatment. The EPG counts also showed
significant reduction on both duration. The
hamatological and biochemical parameters were
monitored.

Development and characterization of indigenous vaccine and diagnostics for johnes
disease
S.V. Singh and Naveen Kumar
Whole genome sequencing
Sequencing of whole genome of native isolate of
Indian Bison Type Biotype of Mycobacterium
avium subspecies paratuberculosis Strain S 5 of
goat origin (CIRG, Makhdoom) was completed
and data was analyzed. Genome size of
indigenous MAP strain, named as Indian Bison
Type and draft sequence reported in Genome
Announcement.
Genome of strain S5 was sequenced by Illumina
GAIIX, which produced a total of 112,487,226
paired-end reads of length 101 nucleotides and
Ion torrent technology, which generated a total
of 1,151,448 reads of length 5 to 202 nucleotides.
NGS QC toolkit v2.2.1 was used to filter the
Illumina data for high quality (HQ) (Cut off read
length for HQ=40%, Cut off quality score=10) and
vector/ adaptor free reads. A total of 100,506,616
paired-end reads and 5,300,026 single end reads
were obtained after filtering and again trimmed
at 3 end (last 11 bases with average quality score
<15). All bases of Ion torrent reads at 3 end were
trimmed with quality score <15. Reference
assisted genome assembly of filtered data was
performed with MAP strain K10 (Genebank
accession: NC_002944.2) using Velvet v1.2.08. A
total of 178 contigs of size 4,798,157 nt with N50
contig length of 58,516 nt; the largest contig
assembled measured 199.4 kb were produced as
draft genome, annotated by RNAmmer 1.2 and
PGAAP (7) pipeline of National Center for
Biotechnology Information (NCBI). A total of
4,288 coding regions (CDSs), 3 rRNAs and 46
tRNAs were predicted.
Genome annotation by PGAAP pipeline showed
that strain S5 contains genes for glycolysis,
gluconeogenesis, pentose phosphate pathway,
tri-carboxylic acid cycle and glyoxylate cycle. A
total of 90 regulator genes were found, which
indicate the ability of strain S5 to survive in a
wide range of environmental conditions. Large
numbers of regulatory genes (~150) were also
found in case of Mycobacterium avium subspecies
paratuberculosis strain K-10(8). There are 18
oxidoreductases and 18 oxygenases present in
the PGAAP annotation, which indicate the role
of strain S5 in lipid metabolism and oxido-
reduction. A total of 4 serine/threonine protein
kinases (STPKs) are also present in the
annotation, which are part the of phosphorelay
system.
The MAP S 5 strain has been transferred to
Veterinary Type Culture Center (VTCC), Hisar
and MTCC, Chandigarh.
Quality test of Indigenous Vaccine for Johnes
disease by the Standardization division of
IVRI, Izatnagar The vaccine vials supplied for
testing belong to batch numbers; PD75aC 12001,
PD75aC 12002 and PD75aC 12003 and vaccine
was found to be safe and sterile by the
Standardization division of IVRI, Izatnagar.
Screening of farm animals for MAP infection
(Johnes disease) by multiple diagnostic tests
Jakhrana unit at CIRG, was reported suffering
with weakness, diarrohea and deaths. Therefore,
all the adult goats (males and females) at the farm
were sampled (118 fecal samples). Goats were
screened for the presence of MAP infection by
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53
microscopy. Of 118 fecal screened by Ziehl
Neelsen staining, 92 (77.9%) were positive for
acid fast bacilli indistinguishable to MAP.
Screening of 17 goats by Indigenous ELISA, 11
(64.7%) were positive for MAP infection.
Fecal samples from suspected Jamunapari goats
(suffering with diarrohea) were sampled and
screened for MAP by microscopy. Of 18
Jamunapari goats screened, 16 (88.8%) were
positive for acid fast bacilli indistinguishable to
MAP and 2, 2, 8 and 4 goats were in +4, +3, +2 and
+1 level shedders, respectively. Of 8 Barbari goats
screened by microscopy, 4 (50.0%) were positive
by microscopy. Muzaffarnagri sheep unit at
CIRG was reported suffering with weakness and
diarrohea. Forty adult sheep (males and females)
of farm unit were sampled (40 fecal and 40 serum
samples). Of 40 fecal samples, 32 (80.0%) were
positive by microscopy for acid fast bacilli
indistinguishable to MAP. Of 40 serum samples,
29 (72.5%) were positive for MAP antibodies.
General screening of farmers goats
Screening of goat farm at Barabanki, Lucknow:
Of 33 goats screened by fecal microscopy, 23
(69.6%) were positive for acid fast bacilli
indistinguishable to MAP using ZN staining.
Of 50 adult sheep (2-2.5 years old) screened, 29
(58.0%) were positive for JD using Indigenous
ELISA kit. Sero-incidence of JD was higher in
adult sheep at Aseda sheep farm.
Screening of cattle and buffaloes from
Dantiwada, Gujarat: Of the 15 animals screened,
6 (40.0%) were positive for JD using Indigenous
ELISA kit.
Of the total 71 [17 (0-18 months), 11 (18-30
months) and 43 (>30 months)], nine calves (2-11
months of age) died showing symptoms of
weakness except one calf which had diarrhea. Of
35 fecal samples screened by microscopy, 24
(68.5%) were positive for Mycobacterium avium
subspecies paratuberculosis (MAP). Screening of
26 serum and 23 milk samples by Indigenous
ELISA kit employing, 24 (92.3%) and 14 (60.8%)
were positive, respectively. Sensitivity of
Indigenous serum ELISA with reference to fecal
microscopy and milk ELISA was 88.2 and 90.0%,
respectively. Screening of blood samples of 14
cows, by specific PCR (IS900), 5 (35.7%) were
positive. Genotyping of PCR positive HF
crossbred cows using IS1311 PCR-REA showed
presence of highly pathogenic Indian Bison
type genotype. Comparison of 3 tests (milk
ELISA, fecal microscopy and IS900 PCR) with
Indigenous serum ELISA revealed substantial
agreement between tests.
Vaccine Therapy to recover dairy farm from
outbreak of JD using indigenous vaccine
Developed vaccine were used in 20 JD positive
cows . Gel (Aluminium hydroxide gel) with 2.5
mg of Inactivated Mycobacterium avium
subspecies paratuberculosis strain S-5genotype
Indian Bison Type in 1 ml of the Alhydro-oxide
gel with 0.01% Thiomersal (antifungal agent)
were used in small ruminants (1 ml sub-
cutaneously) and 2 ml in bovines (cattle and
buffaloes). On the basis of screening of 509
samples from 309 animals belonging to different
livestock farms, the prevalence of MAP was 69.9,
61.2 and 47.6% using microscopy, indigenous
ELISA and IS900 PCR, respectively. Present
findings report very high bio-load of the MAP in
the domestic livestock population screened.
Experimental shed, Animal Health Division,
CIRG, Makhdoom: Goat herds maintained on
Optimum Plane of Nutrition
The vaccine was also tried in different goat farms
in India with encouraging results.

Outreach program on zoonotic diseases : Zoonotic potential of Mycobacterium avium
subspecies paratuberculosis, as the cause of inflammatory bowel (Crohns disease)
in human beings.
S. V. Singh and Naveen Kumar
1054 samples (blood 518, serum 518 and stool
- 18) were collected from 518 individuals from
Agra. Of 518 serum samples collected were
screened by Indigenous ELISA kit and 1.0 and
48.1% were found in strong positive and positive
categories, respectively for Mycobacterium avium
subspecies paratuberculosis (MAP) infection.
Cumulatively, 49.2% human samples were
positive for MAP infection from Agra region.
Study showed significantly higher association of
MAP in cases of hypo-thyroidism (67.0%) and
diabetes (42.6%) as compared to other conditions
(33.3%).
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54
Screening of twenty two human patients
suffering from different types of chronic illness
for MAP infection by Indigenous ELISA kit
revealed that 9.0 and 36.0% samples were in
strong positive and positive categories,
respectively. However, cumulatively here too
45.4% human samples were positive for MAP
infection. Screening of 22 blood samples of these
patients by IS900 blood PCR, 18.1% were positive
for MAP infection. Of 18 stool samples screened
by microscopy, 54.4% were positive for presence
of acid fast bacilli indistinguishable to MAP.
Shedding intensity of MAP was graded as +1, +2,
+3 and +4.. Comparatively stool microscopy was
most sensitive followed by serum ELISA and
blood PCR (table).
Comparative evaluation of Indigenous ELISA kit, IS900 PCR and Microscopy for presence of
MAP infection in IBD/IBS humans ailments
Diagnostic tests Combinations
Indigenous ELISA kit + - + - + + - -
IS900 PCR + - + + - - + -
Microscopy + - - + + - - +
Total (17) 3 (17.6) 3 (17.6) 1 (5.8) 0 (0.0) 5 (29.4) 1 (5.8) 0 (0.0) 4 (23.5)
*Figures in parentheses are percentage.
Some of the chronically sick patients positive for
MAP infection were treated with anti-MAP
therapy and have been followed up for
improvement.
A total of 111 human samples (88 serum and 71
blood) were collected from a health camp
organized by NGO and Gwalior Medical
College, at Chattarpur district of Madhya
Pradesh for screening of human population for
diabetes. Of the 111 suspected human patient s
screened for diabetes, 20 (18.0%) were positive
using commercial kits (ACCU-CHEK test strips).
Of the 88 suspected diabetes patients, 3.4 and
35.2% were positive for MAP infection by ELISA
kit. Of the 88 and 71 healthy suspects screened by
ELISA kit and IS900 PCR, 38.6 and 39.4% were
positive, respectively for MAP infection. And of
19 and 16 diabetes patients screened, by ELISA
kit and IS900 PCR, 31.5 and 43.7% were positive,
respectively for MAP infection. Comparative
evaluation of two tests in 68 suspected human
patients showed that 23.5% persons were
positive for MAP infection by both the tests.
However, 20.5 and 14.7% were positive in ELISA
kit and IS900 PCR, respectively. Comparative
evaluation of two tests in 15 diabetes patients,
showed that 6.6% patients were positive for MAP
infection by both the tests. However, 20.0 and
46.6% were positive in ELISA kit and IS900 PCR,
respectively. In suspected and confirmed cases of
diabetes, blood IS900 PCR was more sensitive as
compared to indigenous ELISA kit. Typing of
IS900 and IS1311 PCR positive MAP DNA
showed that human population was infected
with Indian Bison Type MAP, which is maj or
bio-type infecting animals.
A new trend was noticed with emergence of
Cryptosporidium in adult human and animal
population with clinical disease in association
with infection of MAP. Following fecal / stool
samples from goats, cattle and human beings
were routinely processed for the diagnosis and
monitoring of MAP infection. The samples were
processed for microscopy by rout ine method of
concentration by cerntrifugation and acid fast
staining of the smears and were examined under
100X of the microscope. Results show the
increased presence of heavy (+4) infection of
Cryptosporidium spp., singly or with MAP
infection. These fecal samples were driven from
goats (Etawah), cattle (Ludhiana) and human
(Farah, Mathura) samples and were suspected
for MAP infection and had symptoms of
weakness, constipation, loss in body condition
and diarrhoea. Present study revealed presence
of heavy infection (+4) of Cryptosporidium spp.
Cryptosporidium spp., a single cell parasite has
been associated with cases of diarrhoea in young
age in animals and has also been reported from
young children (Zoonotic) has been found.
However, we have reported two cases of
Cryptosporidiosis in human beings, where
patients suffered with symptoms of IBD. In case
of a teenage girl (16 years) suffered with
symptoms of IBD and was positive for MAP
infection in ELISA, PCR and microscopy (+ 2).
Whereas, an adult boy (22 years) suffered with
chronic constipation for last one year was
exclusively affected with Cryptosporidium .
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Of 24 paneer samples processed for the detection
of MAP, 3 (12.5%) and 1 (4.2%) samples of paneer
fat and sediment, were positive respectively in
microscopic examination.
PCR standardization of five susceptibility gene
for Crohns Disease :
Screening of milk samples and milk products
(Paneer) for presence of MAP: Of 24 paneer
samples processed for the detection of MAP, 3
(12.5%) and 1 (4.2%) samples of paneer fat and
sediment, were positive respectively in
microscopic examination.
Screening of milk samples and milk products
(Paneer) for presence of MAP: Raw milk
samples from rural area to district headquarter
were screened to estimate presence of MAP in
raw and bulk milk tank supplies

Toll like receptors (TLRS) expression and characterization in different breeds of goats
and their role in disease resistance with special reference to Brucellosis
V.K. Gupta, Shivasharanappa N., K. Gururaj, P.K. Rout and Ashok Kumar
PCR, Sequencing and Characterization of goat
TLRs 1, 2 and 3
DNA isolation from blood samples
Blood samples were collected from 4 different
breeds of goats viz., Barbari, Sirohi, Jamunapari,
Jakhrana from the CIRG experimental Goat shed.
The blood samples were subj ected to DNA
isolation using commercial DNA isolation kit.
The concentration and quality of isolated DNA
was checked using Biophotometer.
Primer designing
Primer designing for goat TLR1, 2 and 3 were
done using the available database sequences
from Genbank. Sequences from different
submissions were aligned using ClustalW and
the coding sequence of 2193bp, 2355bp and 2715
bp respectively for TLR1, 2 and 3 were taken for
primer designing and gene amplification.
Multiple sets of oligonucleotide primers
spanning the entire coding region of all the TLRs
from 1-3 (see Table 1) were designed using
FastPCR and validated using OligoAnalyzer 2.0.
Primers for TLR 1-3 in Goat (Capra hircus)
Gene Accession No F/R PRIMER Length
(bp)
Size(bp)
TLR1_F1 NM_001135060.1 F CAGATGCCTGACATCCTCTC
R CGCAGCAGACACTGTGAGAT
20 828
20
TLR1_F2 NM_001135060.1 F CCAACATCTCACAGTGTCTGC
R CAGCCCTCTACCACGTCACT
21 666
20
TLR1_F3 NM_001135060.1 F CAAAGCAGGGAACAATCCAT
R AAATCTCTGTGTAAAGTACTTCTGCTG
20 821
27
TLR2_F1 NM_001048231.1 F ATGCCACGTGCTTTGTGG
R GCTGTAAAATCGCCAACTCC
18 890
20
TLR2_F2 NM_001048231.1 F GGAGTTGGCGATTTTACAGC
R GTCACAGCGGTAGCCATCTG
20 825
20
TLR2_F3 NM_001048231.1 F GACTGGCCAGATGGCTACC
R CTAGGACCTTATTGCAGCTCTC
19 687
22
TLR3_F1 NM_001135928.1 F ATGAGCAGGCCTTTGCCTTA
R TGGCATTGTTCAGAGAGAGG
20 697
20
TLR3_F2 NM_001135928.1 F GGCCTCTCTCTGAACAATGC
R GTGAAGGGGAGCTATCCACA
20 829
20
TLR3_F3 NM_001135928.1 F TGTGGATAGCTCCCCTTCAC
R AACCGAGAACTCGATGCACT
20 749
20
TLR3_F4 NM_001135928.1 F CATTTTGAAGGCTGGAGGAT
R TGTACTGAATTTCTGGACCCAAG
20 539

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Polymerase chain reaction
The TLR sub-genic fragments were amplified
using PCR. The PCR products were purified
using commercial gel elution kit and sequenced
using Sangers dideoxy method.(Fig 2)

Sequence alignment and phylogenetic analysis
The different contiguous fragments that were
sequenced were aligned using Bioedit software
with the existing database sequences. The
contiguous fragments for TLR1, 2 and 3 are three,
three and four respectively.The final coding
sequence was aligned and it contained 2355
nucleotides for all the four breeds of goat TLR2
(see Fig. 3) that were sequenced, followed by
2193 and 2715 nucleotides for TLR1 and 3
respectively. All the four breeds of goats were
aligned with the different species of livestock
and other animals using Clustal W and were
subj ected to phylogenetic analysis using
neighbor j oining (maximum composit e
likelihood) method with molecular evolution
and genetic analysis [Mega 5.2] software as
represented in fig 4. The ORF of TLR2 consisted
of 2355bp and the sequenced information was
submitted to genbank for the breeds Barbari (KF
765736.1), Jamunapari (KJ183648.1), Sirohi
( KJ183650.1) and Jakhrana (KJ183649.1). The
aligned and processed nucleotide sequences
were drawn to phylogeny using maximum
composite likelihood method, and found that all
the four breeds of goats taken in the current
study were in the same clade as of other goats
and sheep. While, the other species that were
found to be closest in clade are exotic cattle and
nilgai. Whereas the primates and rodents falling
much distant in the phylogenetic tree. TLR2
sequences of four different breeds were
sequenced with the idea that the preliminary
data of these indigenous breeds be available for
the researchers. But interestingly the TLR2 was
found to contain considerable nucleotide
variations in the open reading frame of all the
four different breeds.

TLR2 protein homology and 3D modeling
The TLR2 coding sequence of goat was translated
and it was composed of 785 amino acid residues.
The translated sequence was compared with the
other species by aligning them in a SMART
online tool (http://smart.embl-heidelberg.de/) for
identification of signaling domains using protein
domain annotation as depicted in Fig 5.

Fig 2. Showing different TLR fragments
amplified using hi-fidelity PCR
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Table 2. Toll-like receptor genes 1 to 3 sequenced for different breeds of Indian Goats and its Genbank
accession numbers
S.No Gene Breed Accession No. obtained
1.
TLR1
Barbari KJ 210570.1
2 Jamunapari KJ 210567.1
3 Sirohi KJ 210568.1
4 Jakhrana KJ 210569.1
5
TLR2
Barbari KF 765736.1
7 Sirohi KJ 183650.1
9
TLR3
Barbari KJ 210566.1
11 Sirohi KJ 210565.1

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The goat TLR2 protein sequence was analyzed
for its homology by ranking with the closest
template based protein model (Fig. 6) and a three
dimensional structure was designed using
RaptorX online structure prediction server that
identifies 3-state and 8-state secondary structure
of protein along with its solvent accessibility.
Further analysis was aimed at model assisted
protein binding site prediction and Protein
residue contact prediction for TLR2 amino acid
sequence using RaptorX binding site prediction
tool and contact prediction tool respectively. The
nucleotide sequences were translated into
aminoacids to predict the structural and
functional properties of caprine TLR2. All the
four breeds analyzed contained 10 leucine rich
repeats, one LRR-CT and a transmembrane
domain followed by a much conservative Toll
interleukin 1 receptor (TIR) domain. The
ectodomain of predicted TLR2 protein structures
were between aminoacid residues 22 to 574 and
the intracytoplasmic domains were between
residues 624-784 (Fig. 7). The aminoacid changes
in the TLR-2 protein between different Indian
goats against other species of domestic animals
have been compared and identified to foresee
any gross structural changes in the protein
molecule.

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Differential Expression of Toll-like receptors
(TLR-2, 4 and 9) in natural caprine brucellosis
by using real time RT-PCR assay
The differential expression of Toll-like receptors
(TLR 2, 4 & 9) was studied in natural Brucella
melitensis infection in goats. The mammary
gland, supra-mammary lymph nodes (SMLN)
and uterus were collected in RNA later from both
groups after sacrifice. Total RNA was extracted
and quantified. cDNA was synthesized and
quantitative SYBR Green Real Time PCR assay of
TLR-2, 4 and 9 was performed by using specific
primers of bovine and sheep origin. Comparative
Cq method (2-Cq method / Livak method)
was used to calculate the differential expression
in various organs (Fig 9 and 10). The SMLN
showed significantly higher expression of TLR-4
(5 fold), TLR-9 (4 fold) and TLR-2 mRNA (3.5
fold) than that of in control tissues. Mammary
gland showed higher expression of TLR-9 (8
fold) followed by TLR-4 (3 fold) and TLR-2 (2.5
fold). But uterus showed lower expression of
TLR-2 (2 fold), TLR-9 (2 fold) and TLR-4 (1.5
fold). The study indicated that, supra-mammary
lymph nodes followed by mammary gland
elicited strong innate immune response by
expressing higher levels of TLRs when compared
to uterus (See Fig. 11). In this study we also found
that the expression of TLR- 4 and 9 was more as
compared to TLR 2 suggesting their strong role
in innate response against the brucellosis.
TLR-2 Expression in caprine Brucellosis
PBMC Spleen Uterus MG SMLN
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Fig. 9. TLR 2 (left) and 4 (right) mRNA expression in Peripheral Blood Mononuclear Cell (PBMC) spleen, uterus, Mammary
Gland (MG) and Supramammary Lymph Node (SMLN) of Brucella melitensis infected goats. Normalized fold change expression
were assayed by Real Time PCR.The data are expressed as MeanSEM of 3 infected goats at each time point.

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Fig. 10 TLR 2 (left) and 4 (right) mRNA expression in Peripheral Blood Mononuclear Cell (PBMC) spleen, uterus, Mammary
Gland (MG) and Supramammary Lymph Node (SMLN) of Brucella melitensis infected goats. Normalized fold change expression
were assayed by Real Time PCR.The data are expressed as MeanSEM of 3 infected goats at each time point.
TLR 2 TLR 4 TLR 9 IL 1 IL 6 IL 12 INF
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Fig. 11 Expression of different TLRs & cytokines mRNA in different tissues of Brucella infected goats.
Characterization of innate immune receptor following exposure to peste des petits
ruminants virus
Naveen Kumar, S. V. Singh and A. K. Mishra
Peste des Petits Ruminants Virus (PPRV) is
important viral pathogen across the developing
world that places a huge disease burden on
animal industry particularly in small ruminants
and leads to loss of production. Peste des Petits
Ruminants (PPR) leads to high morbidity (10-
83%) and high mortality (10-100%) in sheep and
goat resulting in high economic losses. Adaptive
immune response (generation of virus specific
antibodies and T-cell response) following viral
infection has been well studied but emerging
evidences suggest that the innate immune
system also plays an important role in
modulating the strength, quality and persistence
of adaptive immune response. However, the role
of innate immune system in PPRV replication is
unknown.
The innate immune system can sense viruses,
bacteria, parasites and fungi through the
expression of pattern recognition receptors
(PRRs) which recognize conserved structure in
pathogens called pathogen associated molecular
pattern (PAMPs). The most common PRRs are
toll like receptors (TLRs), retinoic acid-inducible
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61
gene I (RIG I) like receptors (RLRs) such as RIG-
I, Mda-5 and LGP2 and Nod like receptors
(NLRs) (1). Emerging evidences suggests innate
immune system (nature of PRRs triggered) also
plays an important role in modulating the
strength, quality and persistence of adaptive
immune response. Characterization the innate
immune receptors (PRRs) following exposure to
PPRV is unknown which may help identifying
those PRRs that may play a significant role in
generation of persistent antibody and cell-
mediated immune response against PPRV.
Expression of various TLRs (TLR3, TLR7 and
TLR8) in response to exposure of PPRV in Vero
cells was determined by quantitation of the
respective mRNA (TLRs). For amplificastion of
various TLRs gene segments, primers were
designed to select conserved sequences among
human/mice/caprine or bovine by multiple
sequence alignments. Vero cells were cultured in
Minimum Essential Medium (MEM)
supplemented with 10% fetal bovine serum (FBS)
and antibiotics. Confluent monolayers were then
infected with various multiplicity of infection
(moi) (Fig. 1) of PPRV for 1h followed by
washing 5 times with PBS and addition of fresh
MEM. At 2 hpi, cell lysates were prepared to
isolate the total RNA using TRI Reagent as per
the instruction of manufacturer (Sigma,
Steinheim, Germany). mRNA of various TLRs
(Fig 1) was quantified by quantitative real-time
reverse transcription PCR (qRT-PCR). RNA was
cleared of possible DNA contamination by
incubation for 45 min at 37C and 80C for 20
min with DNAse I followed by reverse
transcription. qRT-PCR was carried out with a 20
l reaction mixture containing gene specific
primers and Sybr green DNA dye (Promega,
Madison, USA). The primer pairs used for
amplification of TLRS were: TLR3 forward
primer- 5'- GGC CTT AAT GAA ATT GGG CAA
GAA C -3' and TLR3 reverse primer- 5'- GAC
TCC AAG TTA AGG ATG TGG AGG -3', TLR7
forward primer2 5'- CAA AAC TTC TTG GCC
AAA GAA ATT G -3' and TLR7 reverse primer
5'- GAA GGT GAT ATT TTA TTC ACT GAA AG
-3', TLR8 forward primer- 5'- CCT CAT GCA
GAG CAT CAA CCA AAG CAA GAA AAC -3'
and TLR8 reverse primer 5'- GGC CAC TGG
AGG ATG GAG CTC TT -3'. -actin was used as
a house keeping control gene and was amplified
using forward primer: 5- CCC CAG CCA TGT
ACG TTG CTA TCC -3 and reverse primer: 5-
GCC TCA GGG CAG CGG AAC CGC TCA -3).
For PCR amplification of all the genes (TLR3/7/8,
-actin and PPRV-N gene), initial denaturation
of 95C for 5 minutes followed by 40 cycles of
95C for 30 seconds, 52C for 30 seconds and
72C for 1 minutes and a final extension step of
72C for 10 minutes. As shown in Fig. 1a and b, a
dose dependent enhanced expression of TLRs
(TLR3 and TLR7) was observed following
exposure of PPRV to Vero cells. However, we did
not observe a clean amplification of TLR8; its
further standardization (qRT-PCR) is underway.
Further, we performed a time course experiment
on expression of TLR following exposure of
PPRV to Vero cells. A peak in TLR 3 and TLR7
expression was observed respectively at 1-3
hours post-infection (hpi) (Fig. 2a) and at 1hpi
(Fig 2b).

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Fig. 1: Dose dependent effect of PPRV on TLR expression in Vero cells Vero cells were infected with PPRV at indicatedmoi for
1h, washed 5 times with increasing volume of PBS followed by addition of fresh MEM. At 1 hpi, cells lysates were preparedand
the viral RNA of TLR 3 (a) and TLR7 (b) was quantified by qRT-PCR. -actin was used as a house keeping control gene for
normalisation. Pairwise statistical comparisons to the mock-control group were performed using Students t test. * and **
represent statistical significance at P<0.05 and P<0.01 respectively
Fig. 2: Time course assay: TLR expression in Vero cells following exposure to PPRV Vero cells were infectedwith PPRV atmoi =
5 for 1h, washed 5 times with increasing volume of PBS followed by addition of fresh MEM. At indicated times, cells lysates were
prepared and the viral RNA of TLR3 (a) and TLR7 (b) was quantified by qRT-PCR. -actin was used as a house keeping control
gene for normalisation. Pairwise statistical comparisons to the mock-control group were performed using Students t test. *, **
and *** represent statistical significance at P<0.05, P<0.01 and P<0.001 respectively.
VTC-Veterinary microbes (CIRG-Unit)
V.K. Gupta, A. K. Mishra, K. Gururaj and Naveen Kumar
Cultures submitted by CIRG-VM Unit to VTCC, Hisar
Virus Capripox virus/ CIRG Goat pox virus
Virus Orf virus/ CIRG Orf virus
Virus PPRV/C.hircus-
India/2012/Nanakpurtc/India/2012/Nanakpur
tc/India/2012/Nanakpur
PPR Virus
Bacteria LM1/CIRG Listeria monocytogenes
Bacteria MAP/01/CIRG Mycobacterium avium var
paratuberculosis
Bacteria Pseudo/1/CIRG Psedomonas aeruginosa
Bacteria Sal/1/CIRG Salmonella spp.
Bacteria Esch/1/CIRG Escherichia coli
Bacteria Past/1/CIRG Pasteurella multosida
Bacteria Kleb/1/CIRG Klebsiella pneumoniae
Bacteria Shiga Ecoli/CIRG Shiga toxin producing E Coli

CIRG

63
Isolation, identification and characterization of
different viral and bacterial pathogens of
veterinary importance from goats
A goatpox virus reported in Kanker District of
Chhatisgarh was isolated in primary goat testicle
culture (Fig.) and the identity of the virus was
confirmed as Capripox virus by PCR (Fig).
An Orf virus (ORFV) reported in Barbari goat at
CIRG farm unit (Fig.) was isolated in primary
lamb testicle culture (Fig.) and identified by
amplification of ORFV-specific gene segment in
PCR (Fig.). The phylogenetic analysis (based on
the sequence analysis of envelope protein, B2L)
revealed that the CIRG strain is more closely
related with ORFV strain originated from China.
Peste des Petits Ruminants virus (PPRV) was
isolated from an outbreak that occurred in
Sheep and goats in Nanakpur village of Mathura
District in Uttar Pradesh (India). Based on
hemagglutination with chicken red blood cells
(rbcs), cytopathic effect similar to the
Morbilliviruses in Vero cells, and amplification
and sequence analysis of the viral nucleoprotein
(N) gene, the identity of the virus was confirmed
as PPRV and named PPRV/C. hircustc/
India/2012/Nanakpur1 (in short
PPRV/Nkp1/2012). Isolation and characterization
of native strains of MAP S-5 (Indian Bison
Type) strain of CIRG, Makhdoom was done on
the basis of cultural characteristics and
morphology of bacilli, biochemical, lipid and
protein profiles, RFLP and DNA probes.
Pseudomonas aeruginosa, Klebsiella
pneumoniae Pasteurella multocida, E. coli,
Listeria, Salmonella, Brucella melitensis and
Shiga toxin producing E coli were isolated,
identified and characterized by cultural,
morphological, biochemical and molecular
methods.

Isolation, identification and characterization of major infectious agents associated
with neonatal diarrhoea in kids
Anil Kumar Mishra, Naveen Kumar, K. Gururaj, Souvik Paul and Vinay Chaturvedi
The diarrheic samples (n=210) from the neonatal
kids from Jamunapari, Jakhrana and Barbari
goats were aseptically collected, and
immediately processed for bacteriological
isolation. From the samples, 178 isolates of E. coli
were isolated, identified and characterized. The
molecular identification of E. coli was done by
PCR amplification of the universal stress protein
A (uspA) gene using species specific primers. On
the basis of cultural, morphological, biochemical
and molecular characteristics, the organism was
confirmed as E. coli. Congo red dye agar test was
performed to assess the invasiveness
(pathogenicity) of the E coli isolates. All of the
178 isolates showed 100 % Congo red binding
activity. The identification of shiga toxin
producing E. coli (STEC) or verotoxin producing
E. coli (VTEC) was done by PCR amplification of
stx-1 and stx-2 genes. Out of 178 isolates of E. coli
from the diarrheic neonatal kids, 3.93 % (7/178)
were identified as STEC. The common serotypes
of E. coli responsible for neonatal diarrhoea in
kids were identified as O36, O26, O59, O29, O43,
O91, O82, 9 and O171, out of which, the most
common were O36, O26 and O59. Out of 210
diarrhoeic samples, 16 and 5 isolates of
Salmonella spp. and Klebsiella spp. respectively
were isolated,identified and characterized on the
basis of cultural, morphological, biochemical and
molecular characteristics. In five cases of the
neonatal diarrhoea, Salmonella spp. was found
as single causative agent of the diarrhoea. All
Mock-infected virus infected
134 nt
627 nt
(D)
(C)
(B) (A) (E)
Fig. 2. Isolation and identification of a Goatpox virus: (A) pox lesions (papules) on the body surface in an affected
goat. (B) Pox lesions in lungs. (C) Cytopathic effect of the virus in primary goat testicle culture. (D, E)
Amplificationof Capripoxvirus-specific genesegments inPCR.
1127 nt
M
Mock-inf ected Virus-inf ected
(a)
(b)
(c)
K
i d

A
d
u
l t
N
e
g
a
t
i v
e

c
o
n
t
r
o
l
Kid
Adult
Fig. 1: Isolation and identification of ORFV: (a) Exanthematic gross skin lesions
on the lips, mouth, muzzle and nostrils in the orf affected kids and adults (Barbari
goats). (b) Cytopathic effect of ORFV in primary lamb testes cell culture. (c):
Amplification of the ORFV major envelope glycoprotein (B2L) gene segment in PCR.
CIRG

64
isolates of Klebsiella spp. were isolated
simultaneously with E.coli.Hence, Klebsiella
spp. alone was not found responsible for causing
neonatal diarrhoea in the kids.
Out of 20 diarrhoeic samples, rota virus was
isolated from 1 sample only. The Monkey
Intestinal Epithelial Cells (MA104) were used for
the isolation, purification and propagation of the
virus. Cytopathic effects were observed at 3rd
blind passage in virus-infected cells but not in
mock-infected ones. No bacterial or parasitic
agents were isolated from the sample positive for
rota virus.For detection of the oocysts of
Cryptosporidium in the diarrhoeic faeces in kids,
modified ZN staining method was used. Out of
148 samples, the presence of Cryptosporidium
was found in 46 samples. From 16 samples
positive for Cryptosporidium, no bacterial agent
was isolated indicating its ability to cause the
disease alone.
E coli and Cryptosporidium were found to be
the main causative agents of the neonatal
diarrhea in the kids of Jakhrana, Jamunapari and
Barbari goats. The results also indicated that
Salmonella and Rotavirus were capable to cause
the disease in the kids.

Development of diagnostic assay, molecular characterization and epidemiology of
cryptosporidiosis in goats
Souvik Paul
For diagnosis of cryptosporidium oocysts in kids
faeces Sheathers flotation, NSS sedimentation
and modified ZN staining procedures were
standardized. Total 807 samples were analysed,
out of which 265 were positive for presence of
Cryptosporidium oocysts. Careful analysis of the
above epidemiological observations leads to the
conclusion that Cryptosporidiosis is one of the
main agents responsible for neonatal diarrhoea
in goat kids both under farm and field
conditions. The occurrence of the disease was
found more in farmers flocks which may be due
to lack of hygiene in the premises. The
prevalence of the infection was higher in 0-15
days old kids than 15-30 days old kids. The
prevalence of infection was greater during
summer months than winter months. Sex or
breed had no correlation with the occurrence of
the disease

A B
Cryptosporidium sp oocysts under 100X (A) and 40X(B) magnification in modified Ziehl-Neelsen stained
faecal smears

Metabolic profiling for diagnosis and control of metabolic diseases in goats
Nitika Sharma, Ashok Kumar, Ravindra Kumar, R.V.S. Pawaiya and Vinay Chaturvedi
A total of 135 blood samples were collected from
female goats of Jamunapari (n=54) and Barbari
(n=81) breeds. The status of the negative energy
balance (NEB) in the goats of the aforesaid breeds
was assessed by estimation of glucose (<2mmol/l
glucose), beta-hydoxy butyrate (BHBA >3
mmol/l) and non-esterified fatty acids (NEFA>
0.4 mmol/l) in the serum. Overall prevalence rate
of subclinical ketosis among the goats was
determined as 13.3 % (18/135). The prevalence
CIRG

65
rate in Barbari and Jamunapari does was found
as 17.28 % (14/81) and 7.47 % (4/54) respectively.
The BHBA and NEFA concentration varied from
0.12 to 0.38 mmol/l and 0.166 to 0.89 mmol/l
respectively in healthy peri-parturient does..
However, the number of does with elevated
NEFA concentration (n = 10) was higher than the
number of does with BHBA concentration above
the threshold value (n = 2). These does with
elevated NEFA concentration showed AST
concentrations above the cut off value (>100 U/l).
Thus, NEFA concentration was found to be a
better indicator of NEB in peri-parturient goats.
Spearman correlation tests of the results among
glucose, BHBA and urea concentrations in non-
pregnant does showed a negative correlation
between glucose and urea concentrations (P <
0.01) and positive correlation between BHBA and
urea concentrations (P < 0.05) while no
correlation was observed between BHBA and
glucose concentration. The presence of
significant correlations among serum parameters
in non-pregnant does could be useful to compare
with values in late pregnant does in order to
check pregnancy toxaemia.
Nine cases of death due to pregnancy toxaemia
were diagnosed on the basis of increased
concentrations of BHBA in the aqueous humor of
eye. The method for confirmatory diagnosis of
pregnancy toxaemia in morbid goats by
estimation of BHBA in the vitreous humor was
developed and standardized. Histopathological
studies revealed severe fatty degeneration of the
liver, hypertrophy of adrenal gland and neuronal
necrosis.

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66
Extension Education and Socio-Economics Section
Extension approaches for dissemination of goat production technologies and impact
assessment
Braj Mohan, A.K.Dixit, Khushyal Singh, Vijay Kumar , U.B.Chaudhary and Ashok Kumar
Villages Activities
Total 25 visits were made in adopted villages to
perform different extension activities. A pilot
survey was conducted and basic information
collected. A total of 10 field days, 5 group
discussions, one Scientists-Farmers interaction, a
kisan gosthi, an off-campus training, advisory
services, two Research Extension Farmers -
Interface meetings, 10 health camps organized in
adopted villages. Besides, mineral mixture and
anti-diarrhea powder were distributed to 151
goat farmers.
Socio-Economic Profile
Out of 50 respondents, 66% were landless, 30 %
were marginal and 4% were medium size land
holder whereas average land holding size was
2.1 acre. Maj ority of farmers (72%) belonged to
middle age group (>30-50yrs), 22 % belonged to
old age group (>50yrs) and only 6% belonged to
young age group (30yrs). Average age of
respondents was 469.16 yrs and range varied
from 28-70 yrs. Half of the respondents were
illiterate and 34 % respondents were able to read
or write only. Furthermore, as maj ority of
households (42%) belonged to small family size
(5), 40 % belonged to medium (6-8) and 18%
belonged to large (>8) family size. Average
family size was 7.282.25 and range varied from
3-16. Income status of the household indicated
that 76% of farmers belonged to low income
group followed by medium 20% and high 4%.
Average family income was 56.347.8 thousands.
Similarly, 74% farmers had small flock size (5
goats) followed by medium 24% (6-10 goats) and
large 2% (>10 goats). Average flock size was
4.482.88 goats and range varied from 1-16 goats.

Health and Nutrition
Grazing was important source of nutrition in the
adopted villages. Grazing pattern of goats in the
adopted revealed that the average grazing hours
during summer, rainy and winter was 4.22 hrs,
3.62 hrs and 3.71 hrs, respectively. Adoption of
vaccination and deworming of goats was nil.
Crude mortality was 18.35% at the time of
intervention and maj or cause of mortality was
GIT related problems.

Marketing
Nearly, 48 percent of respondents sell their
animals through middleman and rest followed
direct or other channels. Minimum age of selling
was found 4 moths and maximum was 24
months. Average selling age was 13.4 months.
Minimum selling price of a goat was found Rs.
300 and maximum Rs. 17500 whereas; average
price of a goat was Rs. 5603 in adopted village. It
was found that unit change in average age
(month) the sale price of goatincreased by Rs.
314, it indicates that farmers were getting Rs.314
for keeping a goat for additional one month.

Assessment of economic losses due to diseases in goat production
A.K.Dixit, Braj Mohan, Khushyal Singh, Vijay Kumar, S.K.Singh and Ashok Kumar
A study has been conducted for pre-testing of
schedule and methodology in Jaganpur village of
Auraiya district of Uttar Pradesh where outbreak
of Peste des petits ruminants (PPR) disease was
reported. Data collected through personal
interview method pertaining to village profile,
production system, disease incidence, and
mortality, direct and indirect losses which also
CIRG

67
include opportunity cost incurred due to disease.
The findings from the household survey as also
the focus group discussions in village visited
brought out the role of goat rearing in providing
livelihood security. The maj or findings of this
study were:
Social attributes of respondents
There were 210 households in the village out of
which 178 (85%) households (HHs) reared goats
with an average flock size of five goats.
Social Attributes of Respondents
Attributes Values
Total number of
respondents/HHs
20
Average age of goat
farmers (Yrs)
39.6
Education
Illiterate 3 (15)
Upto middle 8 (40)
Upto high school 6 (30)
Upto intermediate 1 (5)
Graduate & Above 2 (10)
Average family size 6.4
No. of landless goat
farmers
7 (35)
Social class SC
Maj or crops:
Rabi Wheat,
Maize
Kharif Paddy, Jowar
and Baj ra
Income sources (%)
Agriculture 24.44
Animal Husbandry 12.79
Goat rearing 17.32
Others/wages 45.46
Production system Extensive (70%)/Semi-
intensive (30%)
However, 20 affected HHs with an average flock
size of 13 goatswere interviewed to explore the
information on various parameters. Maj ority of
the goat farmers (70%) reared goats under
extensive system. Social attributes of
respondents indicated that 15% of the goat
farmers were illiterates and 65% were educated
up to 10
th
, 12
th
and graduate level. The average
age of the respondents was 40 years and average
family size was of 6. About 35% goat farmers
were landless and maj ority of goat farmers
belonged to schedule cast. The contribution of
goats to family income was about 17%.
Composition of goats and mortality
The composition of flock mainly constituted with
adult (55%) and kids/young stock (45%).The
overall morbidity rate was 57.52% however, the
rate was found slightly higher in young stock
than adults.Similarly, mortality rate in goats in
study households was 42% and case fatality was
reported to 73% (Table). It was high due to delay
in diagnosis and unavailability of veterinary
services during the outbreak, as revealed by the
goat farmers. Economic loss due to mortality was
estimated to be Rs. 12320 per household. Total
morbidity loss due to reduction in milk yield,
weight loss and reduction in market value was
Rs.1567 whereas,the opportunity cost which
include expenses on veterinary care, extra labour
and other charges was estimated to be Rs.
269.The total economic loss due to disease was
Rs. 14156.00.
Morbidity, mortality and case fatality
Particulars Value
Total no. of goats 266
No. of goats infected 153
No. of goats died 112
Morbidity rate (%) 57.52
Mortality rate (%) 42.11
Case Fatality (%) 73.2
Goat management status and constraints
The farmers were asked to assess the knowledge
level on a continuum of poor, average and
good which carried weightage of 1, 2, 3
respectively. The intensity of knowledge and
adoption level were highest with the lower score.
The status of management practices in study
households indicated that about 50% of goat
farmers were rated as poor, 34% as an average
and 16% were as good in knowledge and
management practices which includes kids
management, health, nutrition, breeding and
general management. Furthermore,
unavailability of vaccines,medicines, timely
veterinary services, high cost of medicines and
poor knowledge of diseases and their symptoms
were to be found maj or constraints (Table).

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68
Constraints in goat production
Constraints Mean
Scores
Ra
nk
Unavailability of vaccines 12.19 I
Unavailability of medicines 10.48 II
Lack of veterinary doctors/vet
hospitals
9.12 III
High cost of services and
medicines
8.88 IV
Poor knowledge of diseases and
their symptoms
7.23 V
Poor knowledge of home
remedies/Ayurveda/Herbal
medicines
5.30 VI
Lack of transportation and other
infrastructure
4.27 VI
I

Impact assessment of training programmes on scientific goat farming
Khushyal Singh, Braj Mohan, A.K. Dixit, Vijay Kumar
Data was collected from 98 trainees of 4 states
(Haryana-18, Uttar Pradesh -49, Madhya
Pradesh -10 and Raj asthan-21). Only 25.5%
farmers started their farms and their number are
presented in following diagram:

Survivability of adult goats in the commercial
goat farms was good as per feedback. The kid
mortality was higher in all commercial goat
farms. There were many constraints responsible
for high mortality like low adoption of
improved practices, preventive goat health
calendar, non-availability of critical inputs like
vaccines, medicines and type of housing
Marketing
Middlemen and butchers mainly managed
marketing of goats. Some of the commercial goat
farmers were doing strategic marketing such as
plan for Eid, Holi, Diwali and other local
festivals. They were rearing castrated male as it
gave better prices. The farmers realized
remunerative price for pure breed animals as
compared to non-descript goats.
Knowledge and Adoption of Technology
The level of adoption of technologies was also
not good. Gap in knowledge and adoption of
improved technologies was high due to
inaccessible/unavailablity/ shortage of critical
inputs.

Constraints in goat farming
Technology adoption gap
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80
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CIRG

69
AICRP on goat improvement
S.K. Singh, M.S. Dige, M.K. Singh, P.K. Rout, C. Nimbkar, N. Nahardeka, K.K. Tyagi, P.K. Dogra, S.
Mandakmale, L.B. Singh, D.K. Karna, R.K. Nagda, V. Thirupathy, S.S. Misra, P.K. Senapati, G.C.
Gahlot
Assam Hill Goat Field Unit
The proj ect encompasses 209 farmers families
rearing around 1667 Assam hill Goats. The
population growth has been increased to
121.64% during the year 2013-14. The twining
and triplet percentage has been recorded to be
39.80% and 8.6% respectively and average
mortality rate was reduced from 9.29% to
6.7%.The average family income from the
goatery has increased to Rs. 3,461 /per anum Two
Goat Rally cum Judgings camps were organized
on 12
th
February and 13
th
March, 2014 at Nahira
and Tetelia Field Units of the proj ect respectively.
An exposure visit was organized for 16 farmers
from different field units of the proj ect t o visit
Central Institute for Research on Goats,
Makhdoom, Mathura (U.P) from 21st February
to 2
nd
March, 2014. A total of 5000 doses of frozen
semen of Assam Hill Goat from selected elite
buck has been stored. A total of 16 proven bucks
maintained at the base farm, Goat Research
Station, AAU, Burnihat 793101 has been
distributed replacing the existing bucks in the
four field units of the proj ect. Moreover,
exchange of bucks between the field units is also
practiced on a regular basis. Eighteen (18)
numbers of elite bucks has been distributed to
different NGOs of the state.
Gaddi Field Unit, COVAS, CSKHPKV,
Palampur
In the year under report performance recording
in the four field clusters comprising of 1149 goats
including 749 breedable does following four
different migratory routes were carried out.A
total of 625 young kids were added in selected
flocks through birth, 118 animals of different age
groups died and 459 animals pertaining to
different age groups were sold by the owners for
income generation.The closing balance as on
31.03.2014 was 1197 animals under different age
groups.A total of 25 male kids of 4-6 months age
group were purchased from adopted farmers
after primary selection. These male kids were
then transferred to Palampur center for
subsequent rearing up to the age of sexual
maturity. After final selection, a total of 17 bucks
were distributed to 15 farmers for breed
improvement. All adopted animals were
provided with health coverage under migratory
field conditions and strategic supplementary
feeding was also provided in the form of mineral
mixture and concentrate feed. The overall
population growth was 106.14%.The overall
mortality incidence was found to be 6.65% and
abortion incidences 6.71%.The incidence of twin
birth was recorded 19.96%. The kidding rate of
the flocks were observed to be 1.25%.
Black Bengal (Field Unit) of Birsa Agricultural
University, Ranchi
A new village Tiko having 194 does in Lohardaga
district was adopted during October 2013. Thus,
unit has adopted four village clusters, namely
Palaj ori (Deoghar district), Beko (East
Singhbhum district), Chamguru (Ranchi district)
and Tiko (Lohardaga district) consisting of
approx. 1042 does in coverage areas. Twenty one
elite bucks and 8 does were selected on the basis
of growth and multiple birth to establish the elite
Black Bengal seedstock at the university farm.
Out of these 8 bucks were distributed at new
centre i.e. Tiko village. A total of 872 does kidded
and growth parameters of kids were recorded.
The average litter size was recorded 1.76 and
twining % was observed as 65.23 %. The
breeding efficiency on the basis of does tupped
was estimated to be 182.54 %. Selection
differential was estimated to be 4.02 kg in males
at 9 M of age as compare to previous year 3.39 kg.
The mortality in adult goats and kids were
reduced up to 93.07% in the farmers flock.
Health measure was under taken as vaccination
(PPR, ET, Goat Pox), dipping, drenching etc. as
required. Goat feed prepared by our university
and Mineral mixture were distributed among the
farmers. Two training programmes on goat
husbandry was organized at Goat farm of Ranchi
Veterinary College, BAU, in which 26 goat
farmers from different four centres participated
and benefited by learning by doing on scientific
goat rearing.
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70
Marwari Field Unit, Bikaner, Rajasthan
Four clusters were established in Bikaner
districts i.e. in Deshnok, Kalyansar, Raisar and
Daiya. The new cluster in Kan Singh JiKi Sid
with about 500 goats does in the Jodhapur
district was adopted. The Buck rearing Center is
also functioning at Livestock Research Center,
Kodemdeshar for rearing of elite bucks for
distribution to the farmers. All the registered
goats in new cluster of Kan Singh j iKi Sid were
identified by brass tag and distributed superior
Marwari bucks for breeding purpose.Total 215
adult does and their 75 kids were tagged. The
average family size was 27.8 in this new
cluster.Sixteen selected superior breeding bucks
were placed in the selected cluster for breeding
of does.Total 1296 adult does and their 1396 kids
were recorded. The overall least square mean for
body weights at birth, 3 M, 6 M, 9 M and 12 M of
age were 2.57, 7.96, 14.91, 19.07 and 25.42 Kg,
respectively. Milk yields were recorded more
than 200 does about fortnightly during the
lactation.The kidding take place in all the months
but it was more from the month of October
onwards and it occurs in the open. The overall
kidding percent was 91.50 %, incidence of
abortions was 0.33 % and twinning was 7.71 %.
For all field flock health coverage was provided
besides strategic supplementary feeding in the
form of mineral mixture.
Surti Field Unit, NAU, Navsari
This center was able to establish first notified
goat cooperative of five adopted villages
involving 40 goat farmers through bilateral
efforts of farmers from Valsad District and this
proj ect. Capacity building programme was
undertaken and a 5 day on farm training
program was conducted. Eighteen (18) key
persons have been identified from 14 villages for
the implementation of scheme in their villages.
Six training programmes were organized for the
registered farmers from South Guj arat. They
were given lectures and farm exposure about the
superiority of Surti goat over local breeds. A total
of 12 Surti bucks had been supplied in field to
minimize the problem of non availability of Surti
bucks. Additionally 16 selected bucks are ready
for dissemination this year. Kidding rate had
been increased to 1.46 from 1.41 in 2009.Almost
three fold increase in registered Surti goat
population had been achieved under the proj ect
area. Four additional PG research work and four
ancillary departmental proj ects were undertaken
in the scheme.

Osmanabadi Field Unit, NARI, Phaltan
A new village Borla with about 130 does in
Jamkhedtaluka of Ahmednagar district was
adopted in July 2013. Thus, the unit is now
working in four villages Wadgaon (Satara
district), Kamone (Solapur district), Sakat and
Borla (Ahmednagar district). Goat keepers in
Borla got the benefit of using for breeding
superior Osmanabadi bucks disseminated by the
Osmanabadi Field Unit instead of their earlier
practice of using inferior young bucks. Total 748
adult does and their 1505 kids were recorded
during 2013-14. Milk yields of 400 does were
recorded about 4 times during the lactation.
About 90% of the does older than one year,
kidded during the year and 15-20% of the does
kidded twice in the year. The average litter size
in the four villages was 1.72. The adult and kid
mortality was extremely low i.e. 3 to 4%.
Fourteen selected superior breeding bucks were
placed in the four villages for 3.5 to 8 months for
breeding of does in the village. Additionally,
Osmanabadi buck frozen semen was also made
available in Borla village. Five superior and true-
to-type Osmanabadi bucks were purchased
during the year. For the first time in 2013-14,
fodder seed of the legume Desmanthusvirgatus
and of multi-cut fodder sorghum COFS-29 was
supplied to 16 participating goat keepers in
Wadgaon and Kamone. Four self-help groups
(SHG) of women (61 members in total) were
established in Wadgaon, Borla and Kamone.
These are all operating smoothly with regular
meetings and micro-finance benefits to all
members.
The SHG members of Wadgaon were trained in
goat management and first-aid in evening one-
hour sessions held in their village in the first
week of January 2014. Two visits were organized
for SHG and Pashusakhi group members and
one visit of Wadgaon men goat keepers. About
7,000 straws of frozen semen of 25 Osmanabadi
bucks were produced; 4,000 of these were given
to the Government of Maharashtra and were
supplied by the government to five district AI
centres. Seven hundred straws were given to
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71
field technicians. From their records, an average
50% conception rate was achieved
Black Bengal Field Unit, Kolkata
A new village Bamunia with 119 does in
Bhagabangola Block of Murshidabad district has
been adopted in collaboration with KVK, Digha,
Mursidabad district. Now the BBG, Kolkata unit
is working in three clusters i.e. Ayeshpur and
Ganguria (Nadia cluster), Rangabelia cluster
(South 24 Parganas), Bamunia (Mursidabad
cluster). Goat keepers in all the villages got the
benefit of breeding superior Black Bengal bucks
disseminated by the Black Bengal Field Unit . The
average flock strength of the farmers is 4.49
during 2013-14. Total 451 does and their 934 kids
were recorded during 2013-14. They were
protected with vaccination as per schedule and
deworming and spraying as required. About 83
% of the does kidded once and 17 % of the does
kidded twice during 2013-14. The average litter
size from 526 kidding was 1.78. The kid mortality
was extremely low i.e. 5.39%. Nineteen selected
superior breeding bucks were distributed in the
villages. Twelve Black Bengal bucks have been
distributed in the clusters. Twelve Self-Help
Groups running by women are operating
smoothly with regular meetings and micro-
finance benefits to all members. Seven
deworming cum mineral mixture distribution
camp, 10 treatments cum vaccination camp
(against PPR, Goat Pox and Enterotoxaemia), 11
awareness cum interactive sessions in evening
hour sessions also conducted besides regular
treatment of all goats.The average income per
farmer per year is Rs 4316/- and per doe per year
is 1769/- during 2013-14.
The AICRP on Goat Improvement Centers (XII Plan)
SN Breed Location of Centre Type of Centre
Proj ect Coordinators Unit CIRG, Makhdoom, Farah, Mathura 281122 Coordinating
Unit
Assam Hill Goat Unit
(NEH)
AAU, Khanpara Guwahati Field
Barbari Unit CIRG, Makhdoom Farm
Bengal Goats (TSP) BAU Ranchi Field
Black Bengal (Partial TSP) WBUV and FS, Kolkata Field
Gaddi Field Unit(TSP) HPKVV, Palampur (HP) Field
Ganj am Field Unit OUAT, Bhubaneswar Field
Jamunapari Farm Unit CIRG, Makhdoom Farm
Malabari Field Unit KV&ASU, Thrissur Field
Marwari Field Unit RAJUVAS, Bikaner Field
Osmanabadi Unit NARI, Phaltan (MH) Field
Sirohi Field Unit
(partial TSP)
RAJUVAS,VeterinaryCollegeVallabhnagar
(Raj .)
Field
Surti Field Unit(TSP) N.A.U., Navsari (Guj .) Field
Sangamneri Field Unit MPKV, Rahuri (MH) Field
Sirohi Farm Unit CSWRI, Avikanagar Farm
Newly added Units
Andamani Goats CARI, Port Blair, Andman Field
Himalayan Local Goats IVRICampus,Mukteshwar Field
Changthangi Goat Unit SKUAST-K, Leh, J&K Field
Uttarakhand Local Goats GBPUA&T,Pantnagar Field

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72
Meteorological observations (2013-14)
N. Ramachandran
Months MeanM
ax
Temp.
(
o
C)
MeanMin
Temp.
(
o
C)
MeanDaily
Temp.
(
o
C)
MeanVapo
r
Pressure
(mmHg)
MeanR
H
(%)
MeanRai
nFall
(mm)
/WetDays
Sun
Shine
(hrs)
April2013 40.63 20.85 30.74 11.98 29.21 2 (1) 286.00
May2013 46.31 25.35 35.83 11.92 21.28 0 (0) 304.30
June2013 41.08 28.02 34.55 23.29 33.53 36 (3) 185.70
July2013 36.87 26.98 31.93 27.66 77.41 122 (11) 133.50
August2013 35.60 26.40 31.00 27.11 78.57 117 (15) 157.5
September
2013
37.83 25.17 31.50 23.12 61.80 49 (3) 254.60
October
2013
34.67 20.63 27.65 19.46 63.26 35 (3) 213.70
November
2013
30.35 11.57 20.96 11.08 51.23 0 (0) 185.50
December
2013
25.18 8.58 16.88 10.63 67.06 5.4 (2) 166.10
January
2014
18.50 8.15 13.32 10.59 84.06 30(3) 84.60
February
2014
24.09 9.59 16.84 11.27 69.12 14.40 (3) 171.60
March2013 31.55 14.87 23.21 13.24 52.49 22.60 (2) 261.00
Maximum temperature: 49.5
o
C on 24.05.2013 and 25.05.2013
Minimum temperature: 1
o
C on 30.12.2013
Annual Rain Fall: 433.4 mm in 46 Days
High sunshine: 11.6 hrs on 19.05.13.
Kiddings
Breed Mal e Femal e Total
Barbari 176 151 327
Jamunapari 186 187 373
Jakhrana 59 49 108
Sheep 105 114 219
Total 526 501 1027
Milk production
Breed Milk ( in Kg.)
Barbari 9824.00
Jamunapari 14239.25
Jakhrana 8740.25
NFR&PT Experimental Shed 5267.50
PR&SM Experimental Shed 2134.50
Total 40205.50

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73
Teaching and Training
Teaching
Three postgraduate students from IVRI
completed thesis research work for M.V.Sc
degree. Three students from GLA University
Mathura are conducting research work for PhD
degree under guidance of institute scientist.
Three graduate student from GLAU, Mathura
completed one month summer training and one
PhD student from SHIAT, Allahabad, UP was
given expert guidance on HPLC analysis of plant
extract samples. One batch of BVSC and AH
Students from College of Veterinary science and
AH Mathura completed training under
internship programme. Students of different
academic colleges and veterinary colleges visited
the institute laboratories and livestock units.
Training
The following training programs were organized by the Institute during the year 2013-2014.
Training Programme on Nutrition,
Management and Prevention of goat
diseases for Optimum Productivity Under
Trainers Training Programme of DADF,
Ministry of Agriculture, Govt. of India
Trainees: Veterinary Officers of State A.H.
Department (15)(April 10-16, 2013)
Training Programme on Nutrition,
Management and Prevention of goat
diseases for Optimum Productivity Under
Trainers Training Programme of DADF,
Ministry of Agriculture, Govt. of India ,
Trainees: Veterinary Officers of State A.H.
Department (October 21-29, 2013)
Training Programme on Leadership
Development for Sustainable Goat
Production for Proj ect Officers/Managers of
BAIF, Pune (April 22-27, 2013)
Training programme on Advances in Goat
Production for 10 Veterinary Officers from
Training Institute, Laxmisagar,
Bhubaneswar from December 5-7, 2013
Training Programme for Bank Mangers and
Govt. Officials-One Day Technical Session
and Field visit on Goat Keeping Bankers
Institue of Rural Development (BIRD),
Lucknow on 25 Sept 2013
Training on goat farming to group of
Women, sponsored by Mahila Samakhya
( NGO HRD, Delhi ) under Women
empowerment ( 30.9.2013)Organized a 54th
10 days National Training Programme on
Scientific Goat Farming on 01-10 May, 2013
at CIRG, Makhdoom. In this training
programme 58 Participants (56 farmers and
02 farm women) from 12 States were present.
Organized a sponsored training programme
on Scientific Goat Farming for 07 Veterinary
Officers (05 from Rural Development Deptt.,
Punj ab and 02 from Deptt. Of A.H., Punj ab)
on 20-24 May, 2013 (05 days). Sponsored by
Punj ab State Veterinary Council,
Chandigarh.
on Scientific Goat Farming for 19 farmers on
01-05 July, 2013 (05 days). Sponsored by
Dr. YS Parmar University of Horticulture &
Forestry Reginal Centre, National
Afforestation and Eco-Development Board,
Integrated Watershed Management
Programme, Nauni, Solan, Himachal
Pradesh.
on Scientific Goat Farming for 09 farmers
and 11 farm women (Total = 20 trainees) on
23-27 July, 2013 (05 days). Sponsored by Dr.
YS Parmar University of Horticulture and
Forestry Regional Centre, National
Afforestation and Eco-Development Board,
Integrated Watershed Management
Programme, Nauni, Solan, Himachal
Pradesh.

CIRG

74
Organized a 55th 10 days National Training
Programme on Scientific Goat Farming on
04-13 September, 2013 at CIRG, Makhdoom.
In this training programme 30 Participants
(01 disabled) from 09 States were Present.
on Scientific Goat Farming for 08 Veterinary
Officers from Deptt. Of A.H., Punj ab on 07-
11 October, 2013 (05 days). Sponsored by
Chandigarh.
20-29 November, 2013 at CIRG, Makhdoom.
In this training programme 55 participants
(52 farmers and 03 farm women) from 13
States were present

National Training Programme on Scientific goat farming (10 days duration)
Sr.
No.
Training Period Participants from
No. of states
No. of
trainees
1 54th 01-10 May, 2013 12 58
2 55th 04-13 Sept. 2013 9 30
3 56th 20-29 Nov. 2013 13 55
4 57th 04-13 Mar. 2014 12 58
Sponsored Training Programme on Scientific goat farming
Sr.
N
o
Period Duratio
n
(days)
No. of
Participant
s
Type of
Participan
ts
Sponsoring Agency
1 20-24 May,
2013
5 7 Veterinary
Officers
Chandigarh
2 01-05 July,
2013
5 19 Farmers Dr. YS Parmar University of
Horticulture & Forestry Regional
Centre N. A. E. D. Board, I. W. M.
Programme, Nauni, Soan, Himachal
Pradesh
3 23-27 July,
2013
5 20 Farmers Dr. YS Parmar University of
Horticulture & Forestry Regional
Centre N. A. E. D. Board, I. W. M.
Programme, Nauni, Soan, Himachal
Pradesh
4 07-11 October,
2013
5 8 Veterinary
Officers
Chandigarh.
5 16-20 Dec. 2013 5 25 Farmers ATMA, Sitamarhi, Bihar

6 10-15 Feb. 2014 6 30 Farmers ATMA, Vaishali, Bihar
7 25-29 March
2014
5 34 Farmers GraminVikas Kendra, Nalanda, Bihar
and 06 farm women (Total = 25 participants)
& 01 team leader on 16-20 December, 2013
(05 days). Sponsored by ATMA, Sitamani,
Bihar.
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75
from ATMA, Vaisali, Bihar on 10-15
February, 2014 (06 days) at CIRG,
Makhdoom.
04-13 March, 2014 at CIRG, Makhdoom. In
this training programme 58 participants
(farmers) from 12 States were present
and 06 farm women (Total 34 trainees) from
Distt. Bolangeer, Odisa. Sponsored by
Gramin Vikas Kendra, Nalanda, Bihar on 25-
29 March 2014 (05 days) at CIRG,
Makhdoom.

Exhibition/ Technology Display /Kisan Mela
Participated in Krishi Evam Gramay Vikas
Pradarshani at Pandit Deendayal Dham,
Nagala Chandrabhan, Farah, Mathura,U.P.
on 01-03 October, 2013.
Participated in Krishi Vasant Exhibition at
Central Institute of Cotton Research, Nagpur
on 09-13 February, 2014.
Participated in kisan mela and gosthi at
Jawahar Bagh, Mathura, U.P., on 21.06.2013.
Participated in Showcasing of Agricultural
Technologies j ointly organized by ICAR
Research Complex for Eastern, Patna and
Directorate of Knowledge Management in
Agriculture, New Delhi during December
06-07, 2013 at ICAR Research Complex for
Eastern Region, Patna, Bihar.
Participated in 20th Sarson Vigyan Mela-
cum Exhibition at Directorate of Rapeseed-
Mustard Research (DRMR), Sewar,
Bharatpur (Raj asthan) on 22-24 February,
2014.
Participated in National Dairy Mela-2014 at
NDRI, Karnal (Haryana) on 25-27 February,
2014 (Won IInd Prize).
Participated in Pusa Krishi Vigyan Mela at
IARI, New Delhi on 26-28 February, 2014.
Participated in Kisan Mela at IVRI,
Izatnagar, Bareilly, U.P., on 28.02.2014
(Won IIIrd Prize).

Participated in Kisan Mela at U.P. Pandit
Deen Dayal Upadhyaya Pashu Chikitsa
Vigyan Viswa Vidyalay Evam Go-
Anusandhan Sansthan, Mathura, U.P., on 14-
15 March, 2014 (Won Ist Prize).

Technical Correspondence
Received and replied 133 letters (117 in Hindi
and 16 in English) of different stakeholders on
various aspects of goat production.

Visit Arrangement
1694 visitors were entertained and apprised with
research, extension and development activities of
the Institute during the year.

Helpline Calls
During the year 1785 calls received at CIRG Help
line service regarding various aspects of goat
farming, production, and elite germ plasm and
training programmes etc and replied suitably.

CIRG

Agriculture Farm and Agroforestry Section
Prabhat Tripathi
Agriculture farm section is working with main
obj ectives to produce nutritious fodder for goats
and sheep and to develop ravenous degraded
land of institute in to a fodder production models
through agroforestry or other agricultural
interventions. During the year farm section
supplied 9649 quintals of green fodder to
different livestock units and produced 251
quintals barley& oat grains. Apart from barley
and oat Agril farm section also produced 7.40
quintals of cowpea and guar seed, which will be
utilized for fodder production at farm area of the
institute. About three acre land area was cleaned
and developed. A nursery of about 4000
seedlings (Acacia nilotica, Azadirachta indica,
Syzygium cumini, Ficus lacor, Prosopis cineraria
etc.) was raised and maintained. During the year
about 60% of total supplied fodder was
leguminous with high crude protein percentage.

Proportion of various fodder crops supplied green during the year 2013-14

Farm Area Development Ber( Zizyphus Sp.) Stand
Nursery beds raised at CIRG,
Makhdoom

Mixed
Crops
5%
Berseem
29%
Barley
10%
Sorghum
13%
Oat
9%
Cowpea
19%
Loppings
8%
Maize
4%
Lucerne
3%
CIRG

77
Farm innovators day organized
Institute organized Farm Innovators day on 27
April 2013. Dr. A.K. Mishra, Vice Chancellor,
MAFSU was the Chief Guest and Dr. Rakesh
Babu Gangwar, Deputy Director Agriculture,
Uttra Pradesh was the Guest of Honour on this
occasion. The chief guest of the function while
delivering his inaugural address praised the
efforts made by CIRG in the field of goat
husbandry and informed that goat is one of
oldest animal to be domesticated by man and is
an important animal for providing livelihood
security and alleviating poverty. He accentuated
that interactions like Farm Innovators day will
serve to answer the issues of a sustainable goat
husbandry under the burden of increasing
population, decreasing land and forage
resources and climate change in addition to
entrepreneurship development, reducing
involvement of middlemen in goat marketing,
value addition of goat products etc. The Guest of
Honour in his address emphasized that inputs
like improved breeds, improved nutrition and
better health care facilities should be readily
accessible to farmers for achieving optimum
production in the field of animal husbandry. Dr.
S.K. Agarwal, Director, CIRG presided over the
function and highlighted the importance of goat
husbandry in the rural scenario in terms of
diminishing pastures and grazing land. He
further stressed upon the use of technologies
developed and available at CIRG like artificial
insemination for breed improvement. The farm
innovators day was attended by 167 farmers
including progressive farmers, BAIF officers and
women from Mahila Samakhya, an NGO from
Mathura. On this occasion, few farmers shared
their experiences and local remedies and
innovations related to goat farming. The few
progressive goat farmers were also awarded
with appreciation certificate for sustaining and
motivating other farmers in the field of goat
rearing.

Success Story
CIRG Beans and CIRG-Khasta Goat Milk and Meat Based Products
Goat milk and cream based .CIRG Beans and
CIRG Khasta .were developed using pure goat
.milk, cream, dietary fibres and .natural
antioxidants. These .products contain higher
amount .of medium chain fatty acids, .which are
known to be beneficial .for human health.
Organoleptic .attributes revealed that these
.products has score of 8 out of 9 of .various
parameters under .hedonic scale. Flavour and
colour .of the product was more .appealing to the
sensory .panelists. These products were .snacks
type with low moisture, .high protein and
desirable fatty .acids profile as well as higher
.shelf-life..Another meat based snack food
.CIRG Meat Sticks was .developed with the aim
to .provide good quality protein .a l o n g w i t h
v a l u a b l e .micronutrients to consumers. .These
products are highly .nutritious and palatabl e ,
.enriched with dietary fibre. These technologies
are commercially viable which helps to develop
.small scale industry.

Linkages and Collaborations
The institute has developed effective linkages
with DUVASU, Mathura; IVRI, Izatnagar; NDRI,
Karnal; IARI, New Delhi; CCS HAU, Hisar; Dr.
B.R. Ambedkar University, Agra; CARI,
Izatnagar; NIANP, Bangalore; IGNOU, New
Delhi; CSWRI, Avikanagar; IGFRI, Jhansi and
various Agricultural Universities and NGOs
under AICRP programme. Institute is also
running a proj ect in collaboration with Biovet
Pvt., Bengaluru under Public Private Partnership
programme.

CIRG

78
Technology Services
Goat Germ plasm supplied
CIRG Makhdoom supplied 470 goats and 65
sheep to the progressive farmers and various
government agencies for breed improvement
programmes.

Superior Animal Germplasm Supplied
Breed Total
Jamunapari 213
Barbari 241
Jakhrana 16
Muzzaffarnagri 65
Total 535
Diagnostic Services provided
For the screening of map infection, samples
(serum, fecal) from Veterinary College, Mathura,
Faizabad and Pondicherry and Regional Centres
of CSWRI, Avikanagar (SRC, Kodai Kanal and
WRC, Bikaner) were received. These samples
were screened by ELISA, microscopic
examination, faecal culture and PCR.
Awards and Recognitions
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vk/khu dk;Zjr uxj jktHkk"kk
dk;kZUo;u lfefr (ujkdkl)
eFkqjk }kjk o"kZ 2012&13 ds
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mRd`"V dk;Z gsrq laLFkku dks
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First prize at Kisan Mela U.P. Pt. Deen Dayal
Upadhyaya Pashu Chikitsa Vigyan
Vishwavidyalya evan Go-Anusandhan
Sansthan, Mathura, 14-15 March, 2014.
President , Indian Society for the study of
Animal Reproduction ( S.K. Agarwal)
Vice President, Indian Society for the study
of Sheep and goat Production and Utilization
( S.K. Agarwal)
Vice President , Indian Society for the study
of Animal Reproduction ( S.D. Kharche)
Member , Board of Management, National
Dairy Research Institute, Karnal ( S.K.
Agarwal)
Member , Board of management, MAFSU,
Nagpur (( S.K. Agarwal)
Member, Selection board of GADVASU,
RAJUVAS, JNU, PDC, ASRB, NDRI, IVRI, (
S.K. Agarwal, Ashok Kumar, A.K.Goel,
P.K.Rout, S.K.Singh, R.V.S.Pawaiyya)
Member, IMC, CARI, Izatnagar ( P.K.Rout)

Second Prize at National Dairy Mela-2014 at
NDRI, Karnal (Haryana) on 25-27 February,
2014 .
Third Prize at Kisan Mela at IVRI, Izatnagar,
Bareilly, U.P., on 28.02.2014

CIRG

79

Best Paper Award - All India Scientific and
Technical Hindi Assay Competition.( Saket
Bhusan )
First Prize Hindi Shodh Patra Competetion
held at CIRG ( Chetna Gangwar)
First Prize in Technology dissemination in
Kisan Mela at U.P. Pt. Deen Dayal
Sansthan, Mathura, 14-15 March, 2014.
(Chetna Gangwar)
ISSAR fellowship 2013 (A.K.Goel )
Panelist in a ILRI meet on Small Ruminant
Sector development held at New Delhi
(S.K.Singh)
Member of Management Committee of
NBAGR, Karnal (S.K.Singh)
Third Best Poster Award for Status of bovine
brucellosis and its associted risk factors in
Western Uttar Pradesh. National symposium
and XXVII Annual convention of IAVMI on
Productivity enhancement through
improved animal health and nutrition
Organized by Department of Animal
Husbandry, Government of Uttar Pradesh,
Lucknow from 13-15 December. (Vij ay
Kumar )
Ram Lal Agrawal Gold Medal award -2014
from Indian Society for Veterinary Medicine
at Jammu for outstanding contribution in
Veterinary medicine ,particularly in Herbal
drug Research (Ashok Kumar)
Advisor for World Bank Funded Mega
Proj ect RACP, Jaipur (S.K.Singh)
Member, Sub-committee member on Sheep
Nutrition in the National Committee on
Nutrient Requirement of Animals. by ICAR
(M. K. Tripathi)

First Prizes in Hindi Hastakshar Pratiyogita
and Second Prizes in Shodh Patra
Pratiyogita, Hindi Anuprayog Pratiyogita
and Hindi Shrut Lekh Pratiyogita. ( Gopal
Dass)
Best Reviewer Award 2013 by Asian-
Australasian Journal of Animal Science
( M. K. Tripathi )
Regional Director of Indian sub-continent
International Goat Association ( M. K
Tripathi )
Indian National Science Academy (INSA)
Summer Research fellowship for two months
(Mahesh Shivanand Dige, Shivsharanappa)

CIRG

80
Publications
Research articles
1. Abhishek, Rawat, M. and Mishra, A. K.
(2013). Characterization and lytic activity of
the phage PSA1 induced endolysin LysSA1.
Indian Veterinary Journal 90 :21-22
2. Ajay Singh, Gupta, Vivek, Kumar, Amit,
Singh, V.K. and Shivasharanappa
Nayakwadi (2014). Omp31 gene based
molecular detection of B. melitensis from
serum samples of goats. Indian J ournal of
Animal Science 84 : 251253.
3. Audarya, S.D., Chaubey, K.K., Gupta, S.,
Kumar, N., Yadav, D.S., Chhabra, D., Sharda,
R., Garg, U.K., Singh, S.V. (2013).
Investigation on Bovine Johnes Disease in an
organized dairy farm of Mhow in Madhya
Pradesh. International Journal of Current
Research 5: 2895-2897.
4. Baithalu Rubina Kumari, Singh S.K., Gupta
Chhavi, Raja Anuj K., Saxena Abhishake,
Kumar Yogendra, Singh R. and Agarwal S.K.
(2013) Cellular and functional
characterization of buffalo (Bubalus bubalis)
corpus luteum during estrous cycle and
pregnancy. Animal Reproduction Science.
140: 138-146
5. Baithalu Rubina Kumari, Singh S.K., Gupta
C., Raja Anuj K., Saxena A and Agarwal S.K.
(2013) Insulin stimulates progesterones
secretion to a greater extent than LH in early
pregnant buffalo luteal cells cultured in vitro.
Animal Reproduction Science. 142: 131-136
6. Barad, D.B., Chandel, B.S., Dadawala, A.I.,
Chauhan, H.C., Kher, H.N., Shroff, S.,
Bhagat, A.G., Singh, S.V., Singh, P.K., Singh,
A.V., Sohal, J.S., Gupta, S., Chaubey, K.K.
Chakraborty S., Tiwari R., Deb R., Dhama K.,
(2014). Incidence of Mycobacterium avium
subspecies paratuberculosis in Mehsani and
Surti goats of Indian origin using multiple
diagnostic tests. Journal of Biological
Sciences 14: 124-133.
7. Barad, D.B., Chandel, B.S., Dadawala, A.I.,
Chauhan, H.C., Kher, H.N., Shroff, S.,
Bhagat, A.G., Singh, S.V., Singh, P.K., Singh,
A.V., Sohal, J.S., Gupta, S., Chaubey
,K.K.(2013). Comparative Potential of
Traditional versus Modern Diagnostic Tests
in Estimating Incidence of Caprine Johnes
disease. Advances in Animal and Veterinary
Sciences 1: 35-40.
8. Bari, Nilesh, Kumar, Naresh, Singh, Renu,
Kumar, Kuldeep, Ranjan, R., Singh, R. K.,
Yasotha T., Kumar, Manish, Panda, R. P.,
Das , B. C., Sarkar M. and Bag Sadhan.
(2013). Effect of extract egg supplementation
on expression of mushashi gene in caprine
neural stem. Indian Journal of Animal
Sciences 83: 533535.
9. Bhusan, S. (2013). Genetic improvement of
production and reproduction traits in goats
of Jakhrana breed (in hindi). Vartmaan
Vaigyaanik Anusandhan, DRDO, Ministry of
Defence 3: 124-127.
10. Chakraborty, S., Kumar, N, Dhama, K, Verma
A. K., Tiwari, R., Kumar A, Kapoor S and
Singh S V. (2014). Foot and Mouth Disease; an
economically important disease of animals.
Advances in Animal and Veterinary Sciences.
2 (2S): 1-16.
11. Chaudhary, U.B., Tripathi, M.K., Gupta
Bhavna, Dutta, T.K. and Sirohi, H.V. (2013).
Effect of inorganic and organic zinc and
copper on performance, nutrient utilization,
rumen fermentation and blood biochemistry
of kids. Indian Journal of Animal Sciences 83:
1313-1322.
12. Das, Arun K. and Rajkumar, V. (2013). Effect
of different fat level on microwave cooking
properties of goat meat patties. Journal of
Food Science and Technology 50: 1206-1211.
13. Dikshit, A. K. and Birthal, P. S. (2013).
Positive Environmental Externalities of
Livestock in Mixed Farming Systems of
India: Agricultural Economics Research
Review 26: 21-29.
14. Dixit, M., Filia, G., Singh, S.V., Gupta, M.P.
(2013). Prevalence of Paratuberculosis in
small ruminants in Punjab. Indian Veterinary
Journal 90 : 25-26.
15. Goel, A. K., Sharma, R.B. and Singh, M. K.
(2013). Impact evaluation of goat production
technologies in farmers flock. Indian Journal
of Animal Sciences 83: 550-553.
16. Gupta V. K., Nayakwadi S., Kumar A.,
Gururaj K., Kumar A. and Pawaiya R.V.S.
(2014). Markers for the molecular diagnosis
of brucellosis in animals. Adv. Anim. Vet. Sci.
2 : 31 39.
17. Gupta, V. K., Shivasharanappa, N., Kumar, V.
and Kumar, A. (2014). Diagnostic evaluation
CIRG

81
of serological assays and different gene based
PCR for detection of Brucella melitensis in
goat, Small Ruminant Research 117: 94-102.
18. Gururaj, K., Kirubaharan J.J., Gupta V.K.,
Pawaiya R.V.S., Shivasharanappa N. and
Mishra A.K. (2014). Past and present of
reverse genetics in animal virology with
special reference to nonsegmented negative
stranded RNA viruses; a review. Adv. Anim.
Vet. Sci. 2: 40 48.
19. Jerome A, Singh S.K. and Agarwal S.K (2013)
Interaction analysis of buffalo pregnancy
associated glycoprotein-1 in silico. Indian
Journal of Animal Science 83 (11): 1149-1154.
20. Jessiehun Nongsiej, Singh S.K., Sharma
Chethan G, Rakesh H.B, Singh R.P., and
Agarwal S.K. (2013) Freezing of endometrial
epithelial cells of buffalo cultured in-vitro.
Indian Journal of Animal Sciences 83(7):742-
43.
21. Kandasamy, S., A. Jain, P. Baviskar, R.
Kumar, P. Joshi, S.K. Agarwal, A. Mitra
(2013). Molecular characterization and
expression profile of ghrelin gene during
different reproductive phases in buffalo
(Bubalus bubalis) Domestic Animal
Endocrinology 45 : 55-63.
22. Kaur, G, Khandelwal, N, Kumar, N and
Tiwari, A. (2013). Revival of medicinal plants:
channeling the traditional uses towards
modern applications. International J.
Indigenous Medicinal Plants 46: 1196-1205.
23. Khandelwal, N., Kaur, G., Kumar, N. and
Tiwari, A. (2014). Application of silver
nanoparticles in viral inhibition: a new hope
for antivirals. Digest Journal of
Nanomaterials Biostructures:9:175-186.
24. Kharche, S.D., S.K.Jindal, R. Priyadhrashini,
Kumar Satish, Goel, A. K., Ramachandran N.
and Rout, P.K. (2013). Fertility following
frozen semen artificial insemination in
Jamunapari goats. Indian J ournal of Animal
Sciences 83: 1071-1073.
25. Kharche, S.D., Goel, A.K., Jindal, S.K., Jha,
B.K. and Goel P. (2013).Assessment of
Parthenogenetic Embryo Production by
Activation of In-vitro Matured Caprine
Oocytes with Different Concentrations of
Ethanol. Small Ruminant Research 111: 100-
103.
26. Kopp, C., Singh, S. P., Regenhard P.,
Sauerwein H. and Mielenz M. (2014). Trans-
cinnamic acid increases adiponectin and the
phosphorylation of AMP-activated protein
kinase via G-protein coupled receptor 109A
in 3T3-L1 adipocyte. International Journal of
Molecular Sciences 15: 2906-2915.
27. Kumar Ajay, Rout, P. K., Mohanty B. P.
(2013). Identification of Milk Protein
Polymorphism in Indian Goats by 2D Gel
Electrophoresis. J ournal of Proteomics and
Bioinformatics 6: 001-004.
28. Kumar Kuldeep, Singh Renu, Ranjan R.,
Yasotha T., Singh, R. K., Kumar Manish,
Bhanja, S. K., Das, B. C., Mohan, N. H. and
Bag Sadhan. (2013). Use of extract egg for
parthenogenetic embryos development in
caprine. Indian Journal of Animal Sciences
83: 377378.
29. Kumar Manish, Yasotha T., Singh, R. K.,
Singh, Renu., Kumar Kuldeep, Ranjan, R.,.
Das, B. C., Chetan D., Meshram, M. S. and
Bag Sadhan. (2013). Generation of transgenic
mesenchymal stem cell expressing green
florosencent protein as reporter gene using
no viral vector in caprine. Indian Journal of
Experimental Biology 51: 502-509.
30. Kumar, Naveen, Chaubay KK, Singh, SV,
Gupta S, Sharma S, Sharma DK, Mishra AK
and Singh MK. (2014). Phylogenetic analysis
of an Indian orf virus isolated from an
outbreak in an organized sheep flock at
Makhdoo, India. Virus Genes 48: 312-319.
31. Kumar P.R., Singh, S.K., Kharche, S.D.,
Chethan Sharma, G, Behera, B.K., Shukla,
S.N., Kumar ,H. and Agarwal, S.K. ( 2014).
Anestrus in cattle and buffalo: Indian
perspective. Adv. Anim. Vet. Sci. 2 : 124 138.
32. Kumar, R., Chaudhary, U.B., Kumar, A.,
Sharma, D.K., Tripathi, P. and Tripathi, M.K.,
(2014). Effect of feeding complete pellet
containing herbal anticoccidial herbal feed
mix on performance of Barbari goats. Indian
Journal of Small Ruminants 20 (1): 64-66.
33. Kumar, V. (2013). Impact of print-media on
helpline service. Indian J ournal of Social
Research 54: 285-288.
34. Kumar, V., Singh, B.P. and Bangar, Y.C.
(2013). Adoption level of breeding and
reproduction management practices among
goat farmers in semi arid zone of Uttar
Pradesh. Ruminant Science 2: 207-210.
35. Kumar, Naveen and Maherchandani, S.
(2014). Targeting host cell factors for
development of antiviral therapeutics. Adv.
Anim. Vet. Sci. 1S: 37.-41.
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36. Kumar, Naveen, Singh, S.V., Sharma, S.,
Chaubey, K.K. (2014). Peste des Petits
Ruminants virus in small ruminants. A
review. Viruses: DOI:10.3339.v60.
37. Kumar, R., Singh, B.P., Kumar, V., Bangar, Y.
& Maousami (2013). Perceived sustainability
of health technologies by goat farmers in
different agro-climatic zones of Bihar. Journal
of Applied Animal Research 42: 200-203.
38. Kumar, T., Singh, R.V., Sharma D., Gupta S.,
Chaubey K.K., Thakur S., Rawat K.D., Kumar
N., Dhama K., Tiwari R., Singh S.V. (2014).
Status of Mycobacterium avium subspecies
paratuberculosis infection in an Indian
goshala housing poorly or unproductive
cows suffering with clinical Bovine Johnes
disease. Advances in Animal and Veterinary
Sciences. 2: 261-263.
39. Kushwah, Y. K., Dass, G., Rout, P. K. and
Sharma, D. K. (2013). Milk protein
polymorphism in Jamunapari goats. Indian
Res. J. Genet. & Biotech 5: 203-212.
40. Mamata, Dutta T.K., Tripathi, M.K. (2014).
Effect of levels of concentrate on rumen
fermentation and methane production in-
vitro and in weaned Barbari kids. Indian
Journal of Small Ruminants.20: 24-30.
41. Mandal, A., Tomar, S. S., Roy, R. and Rout, P.
K. (2013). Genetic studies of growth traits in
Jamunapari goats of India. Indian Journal of
Dairy Science, 66(3): 130-133.
42. Mandal, A., Dass, Gopal and Rout, P.K.
(2013). Genetic polymorphism of milk
proteins Indian Veterinary Journal, 90 (1):
110-111.
43. Maousami, Singh, B. P., Kumar R., Kumar V.
and Dohare A. (2013). Analysis of Buffalo
Calf Management Practices followed by
Buffalo Owners. Journal of Animal Science
Advances 3: 129-133.
44. Mielenz, M., Mielenz, B., Singh, S. P., Kopp,
C., Heinz, J., Hussler, S. and Sauerwein H.
(2013). Development, validation, and pilot
application of a semiquantitative Western
blot analysis and an ELISA for bovine
adiponectin. Domestic Animal
Endocrinology 44: 121130.
45. Mir, I.A., Kumar, Ravindra., Ahmad, T., and
Sheikh, F.A. (2013) Effect of dietary
supplementation of Raw Fenugreek seeds
(Trigonella foenun-graecum) on blood
biochemical profile in Goats. Indian Journal
of Animal Nutrition 30 (3):242-245.
46. Mishra, A. K., Sharma, N., Kumar, N.,
Shivasharanappa, N., Gururaj, K., Abhishek,
Paul, S. and Chaturvedi, V.( 2013).Prevalence
and etiology of subclinical mastitis in
Jamunapari and Barbari goats. Journal of
Immunology and Immunopathology 15 : 142-
142.
47. Mishra, A.S. Tripathi, M.K., Vaithiyanathan,
S. and Jakhmola, R.C. (2013). Nutritional
evaluation of fallen Tree leaves as source of
roughage in complete feed blocks for sheep.
Animal Nutrition and Feed Technology 13:
223-234.
48. Nayakwadi S, Gupta VK, Pawaiya RS, Reddy
GBM, Kumar A, Barbuddhe SB (2014).
Isolation and characterization of Listeria
monocytogenes from goat brain. Adv. Anim.
Vet. Sci. 2 : 12 15.
49. Nazir G., Ghuman S.P.S., Singh J.,
Honparkhe M, Ahuja C.S., Dhaliwal G.S.,
Sangha M.K. Jaijpaul, Agarwal S.K. (2013)
Improvement of conception rate in post
partum flax seed supplemented buffalo with
Ovsynch +CIDR protocol. Animal
Reproduction Science 137: 15-22.
50. Pal, R.S., Singh, K.S., Tripathi, M.K. (2013).
Effect of poultry litter inclusion in
concentrate mixture on nutrient digestibility
and milk production in lactating cows.
International Journal of Current Research. 5:
1217-1230.
51. Pandey A.K., Ghuman SPS, Dhaliwal G.S. ,
Kumar, Ajeet and Agarwal S.K. (2013) Impact
of buserelin acetate or hCG administration on
day 12 post-ovulation on subsequent luteal
profile and conception rate in buffalo
(Bubalus bubalis) Animal Reproduction
Science136:260-267
52. Pathak, J., Kharche, S. D., Goel, A. K. and
Jindal, S.K. ( 2013). A comparative study on
parthenogenetic activation and embryo
production from in vitro matured caprine
oocytes Small Ruminant Research 113 :136-
140.
53. Paul S , Manjunatha Reddy, G.B and Sharma
D.K. (2013). Cryptosporidiosis in neonatal
goats kids in North -Western India. Indian
Veterinary Journal, 90 (4): 142-143.
54. Paul S, Tiwari AK, Sharma DK, Mishra AK,
Shivasharanappa N, Nitika Sharma, Gupta
VK, Ashok Kumar, Vinay Chaturvedi and
RVS Pawaiya. (2013). Sero-prevalence and
molecular detection of Toxoplasma gondi
isolates from goats in semiarid region of
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India. Journal of Immunology and
Immunopathology 15: 79.
55. Paul, S. and Sharma, D.K. (2014). Amprolium
resistant Eimeria species in goats. Indian
Veterinary Journal 91:89-90.
56. Paul, S., Sharma D.K., Boral, R., Mishra A.K.,
Shivsharanappa, N., Banerjee P.S. and
Pawaiya R.V.S. (2014). Cryptosporidiosis in
goats; a review. Adv. Anim. Vet. Sci. 2: 49
54.
57. Pawaiya, R. V. S. and Gupta, V. K. (2013). A
review on Schmallenberg virus infection: a
newly emerging disease of cattle, sheep and
goats. Veterinrn Medicna 58: 516-526.
58. Raghuvanshi, T., Singh, S.V., Sharma, R.B.,
Gupta, S., Chaubey, K.K., Kumar, N. and
Dhama, K. (2013). Identification of
mycobacterium Avium subspecies
Paratuberculosis in fresh cheese (paneer)
from goat herds endemic for Johnes disease.
Journal of Infection and Molecular Biology
1: 46 48.
59. Rai, B., Singh, M. K., Dixit, A. K. and Rai, R.
B. (2013). Livelihood security through
improved goat rearing practices under field
conditions. Indian Journal of Small
Ruminants 19: 198-201.
60. Rakesh H.B., Singh S.K., Sharma Chethan G.,
Jessiehun N. and Agarwal S.K. (2013)
Morphological and functional
characterization of corpus luteum during
different stages of estrous cycle in buffalo.
Indian Journal of Animal Sciences 83(7):710-
12.
61. Ranjan, R., Singh, R. K., Yasotha, T., Kumar,
M., Puri, G., Kumar, K., Singh, Renu.,
Bhure, S., Malakar, D., Bhanja, S., Sarkar,
K. M., Das, B. C. and Bag, S. (2013). Effect
of Actin Polymerization Inhibitor During
Oocyte Maturation on Parthenogenetic
Embryo Development and Ploidy in Capra
Hircus. Biochem Genet. 51: 944-953.
62. Ranjan, R., Das, B. C. and Bag Sadhan. (2013).
Molecular sexing of IVF and in vivo derived
embryonic cell colony in goat. Indian Journal
of Animal Sciences 83: 10391041.
63. Ranjan, R., Das, B. C. and Bag Sadhan. (2014).
Effect of cytochalasin B during oocyte
maturation for parthenogenetic embryos
generation in goat Indian Journal of Animal
Sciences 84: 271274.
64. Ranjan, R., Singh, R. K., Yasotha, T., Kumar,
M., Kumar, K., Singh, Renu., Houque, M.,
Mourya,V. P., Singh, G., Sarkar, M., Das, B.
C. and Bag, Sadhan. (2013). Survivability of
parthenogenetic embryos following in vivo
transfer in naturally synchronized Capra
Hircus. In Vitro Cell.Dev.Biol. (Animal)
49:486491.
65. Sachan, J., Kumar Ravindra, Vaswani, S.
Kumar V. and Roy D. (2014) Effect of addition
of herbs on in vitro rumen fermentation and
digestibility of feed. Indian Journal of Animal
Research 48 (1) 91-93.
66. Saraswat, S., Jindal, S. K., Kharche, S. D.,
Rout, P. K., Ranjan, R. and Priyadharsini, R.
(2014). Role of antioxidant additives in the
protection of DNA integrity of buck
spermatozoa with RAPD assay. Indian
Journal of Animal Sciences 84: 295297.
67. Sharma N, Mukherjee R and Mishra A K
(2013). Assessment of therapeutic potential of
autogenous Staphylococcus aureus bacterin
plus Vitamin E and Selenium in mouse model
mastitis. Indian Veterinary Journal, 90 (50):
38-39.
68. Sharma N, Shivasharanappa N, Pawaiya
RVS, Kumar S, Mishra A K, Kumar A,
Chaturvedi V, Paul S and Tailie W A. (2014).
Parasitic Encephalomyelitis in Goats due to
aberrant infestation with botfly Oestrous ovis
larvae. Adv Anim Vet Sci 2: 8-11.
69. Sharma Nitika, Ashok Kumar, Ravindra
Kumar, Shivasharanappa N, Mishra A K,
Ashish Shrivastav and Chaturvedi V. (2013).
Thiamine responsive polioencephalomalacia
in a kid. Veterinary Practitioner 14: 325-326.
70. Sharma Nitika, Reena M, Shivasharanappa
N, AK Mishra, Souvik Paul and Vinay
Chaturvedi (2013). Evaluation of Immune
response of autogenous Staphylococcus
aureus bacterin in mouse model mastitis.
Journal of Immunology and
Immunopathology, 15:95.
71. Sharma, R.B., Raghuvanshi, T.S., Hussain, T.,
and Singh, S.V. (2014) Immune activation in
HIV patients fed with goat milk before and
after initiation of anti-retroviral therapy. The
Indian Journal of Small Ruminants 20: 67-70
72. Sharma, A., Nayakwadi, S., Chandratre, G.,
Saini, M., Das, A., Raut, S., Swarup, D., and
Somvanshi, R. (2014). Prevalence of
Pathological Conditions in Zoo/Wild
Animals in India: A Retrospective Study
Based on Necropsy. Proceedings of the
National Academy of Sciences, India Section
B: Biological Sciences, 1-10.
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73. Shome , R., Gupta, V. K., Rao, K. N., Shome,
B.R., Nagalingam, M. and Rahman, H.
(2013). Detection of Brucella melitensis Rev
1 Vaccinal Antibodies in Sheep in India. Adv.
Anim. Vet. Sci. 2 : 19-22.
74. Shome, R., Gupta, V. K., Bhardwaj, B.,
Shome, B.R., Nagalingam, M. and Rahman,
H. (2014). A report of seroprevalence of camel
brucellosis in India. Journal of Camel
Practice and Research. 20 :183-186.
75. Shroff, S., Chandel B. S., Dadawala A. I.,
Gupta S., Chaubey K.K., Singh S. V. (2014).
Incidence of Mycobacterium avium subspecies
paratuberculosis in Patanwadi sheep. Indian
Journal of Small Ruminants 20: 54-58.
76. Shroff, S., Chandel B.S., Dadawala A.I., Singh
S.V., Bhagat A.G., Chauhan H.C., Gupta S.
(2013). Evaluation of Indigenous vaccine in
Patanwadi sheep naturally infected with
clinical Johnes disease. Research Opinions in
Animal & Veterinary Sciences, 3: 322-329.
77. Singh Ajay, Gupta V. K., Kumar Amit, Singh
V. K. and Shivasharanappa N. (2014). Omp31
gene based molecular detection of B.
melitensis from serum samples of goats.
Indian J of Animal Science. 84: 251253.
78. Singh Ajay, Gupta, V. K., Kumar Amit, Singh
V. K. and Shivasharanappa N.(2013). 16S
rRNA and Omp 31 Gene Based Molecular
Characterization of Field Strains of B.
melitensis from Aborted Foetus of Goats in
India. http://dx.doi.org/10.1155/2013/160376.
79. Singh, A.K., Verma, A.K., Neha , Tiwari, R.,
Karthik, K., Dhama, K., & Singh, S.V. (2014).
Trends and Advances in Vaccines Against
Protozoan Parasites of Veterinary
Importance: A Review. Journal of Biological
Sciences 14: 95-109 .
80. Singh, Gitam and Sharma, R.B. (2013).
Influence of breeds on goat milk composition
under field and farm rearing conditions.
Indian Research Journal of Genetics and
Biotechnology 5: 258-261.
81. Singh, K., Chandel B.S., Dadawala A.I., Singh
S.V., Chauhan H.C., Singh B., Agrawal N.D.,
Gupta S., Chaubey K.K. (2013). Incidence of
paratuberculosis in Mehsana Breed of Goats
from North Gujarat using Multiple Tests.
Advances in Animal and Veterinary Sciences
1: 28-31.
82. Singh, K., Chandel, B.S., Chauhan, H.C.,
Dadawala, A., Singh, S.V., Singh, P.K. (2013).
Efficacy of Indigenous Vaccine using native
Indian Bison Type genotype of
paratuberculosis for the control of clinical
Johnes disease in an organized goat herd.
Veterinary Research Communications 37:
109-114.
83. Singh, M. K., Dixit, A K, Roy, Roy A K and
Singh, S K (2013). Goat Rearing: A Pathway
for Sustainable Livelihood Security in
Bundelkhand Region: Agricultural
Economics Research Review 26:79-88
84. Singh, P.K., Singh S.V., Saxena V.K., Singh
M.K., Singh A.V. and Sohal J.S. (2013).
Expression profile of different cytokine genes
in peripheral blood mononuclear cells of
goats infected experimentally with native
strains of Mycobacterium avium subspecies
paratuberculosis. doi:
10.1080/10495398.2012.762008.
85. Singh, R., Kumar, K., Khan, I., Ranjan, R.,
Yasotha, T., Singh, R. K., Das, B. C. and Bag,
S. (2013). Comparative expression profile of
developmental related genes in haploid and
diploid parthenogenetic embryos in caprine.
Indian Journal of Animal Sciences 83 : 502
505.
86. Singh, R., Kumar, K., Ranjan, R., Kumar, M.,
Yasotha, T., Singh, R. K., Das, B. C., Sarkar,
M. and Bag, S. (2013). Comparative
expression analysis of embryonic
development related gene at different stages
of parthenogenetic and in vitro fertilized
embryos in caprine. Zygote 11: DOI
10.1017/S096719941300049X.
87. Singh, S. P., Hussler, S., Gross, J. J.,
Bruckmaier, R. M., and Sauerwein, H. (2014).
Circulating and milk adiponectin change
differently during energy deficiency at
different stages of lactation in dairy cows.
Journal of Dairy Science 97: 1535-1542.
88. Singh, S. P., Hussler, S., Heinz, J. F. L.,
Saremi, B., Mielenz, B., Rehage, J., Dnicke,
S., Mielenz, M., and Sauerwein, H. (2014).
Supplementation with conjugated linoleic
acids extends the adiponectin deficit during
early lactation in dairy cows. General and
Comparative Endocrinology 198: 13-21.
89. Singh, S. P., Hussler, S., Heinz, J. F. L., Akter,
S. H., Saremi, B., Mller, U., Rehage, J.,
Dnicke, S., Mielenz, M. and Sauerwein, H.
(2014). Lactation driven dynamics of
adiponectin supply from different fat depots
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85
to circulation in cows. Domestic Animal
Endocrinology 47: 35-46.
90. Singh, S. V., Chaubey, K. K., Gupta, S.,
Gupta, V. K., Agrawal, N. D. and Kumar, N.
(2013). CoInfection of Mycobacterium
avium Subspecies paratuberculosis and
Brucella melitensis in a Sirohi Breed of Goats
in India. Adv. Anim. Vet. Sci. 1 : 188 190.
91. Singh, S. V., Gupta, S., Chaubey, K. K., Rawat,
K. D., Kumar, N., Sohal, J. S., Singh, S.,
Tiwari, R., Chakraborty, S. and Dhama,
Kuldeep. (2014). Johne's Disease (JD) in a
High Yielding Holstein Friesian Cattle Dairy
Farm in India. Journal of Biological Sciences
14: 195-203.
92. Singh, S.V., Dhama K., Chaubey K.K., Kumar
N., Singh P.K., Sohal J.S., Gupta S., Singh
A.V., Verma A.K., Tiwari R., Mahima
Chakraborty S. and Deb R. (2013). Impact of
Host Genetics on susceptibility and
resistance to Mycobacterium avium
subspecies paratuberculosis in domestic
ruminants. Pakistan Journal of Biological
Science 16 : 251-266.
93. Singh, S.V., Gupta S., Chaubey K.K., Kumar
N., Rawat K.D., Tiwari R. and Dhama K
(2014). Dose Standardization Studies of
Indigenous Vaccine for the Control of
Mycobacterium Avium Subspecies
Paratuberculosis in Naturally Infected Goats.
2: 17-22.
94. Singh, S.V., Gupta S., Singh P.K., Sohal J.S.,
Kumar N., Kumar A., Chaubey K.K., Singh B
(2013). Prophylactic study of Indigenous
vaccine against Johnes disease in dairy cow
and male calf of Hariana breed: Case study.
1: 23-28.
95. Singh, S.V., Gupta V.K., Kumar A., Gupta S.,
Tiwari R. and Dhama K. (2014). Therapeutic
Management of Bovine Brucellosis in
Endemically Infected Dairy Cattle Herd of
Native Sahiwal Breed. Advances in Animal
and Veterinary Sciences 2: 32-36.
96. Singh, S.V., Kumar N., Chaubey K.K., Gupta
S., Rawat, K.D.(2013). Bio-presence of
paratuberculosis infection in domestic
livestock farms. Research Opinions in Animal
and Veterinary Sciences 3 :401-406.
97. Singh, S.V., Kumar N., Singh S.N.,
Bhattacharya T., Sohal J.S., Singh P.K., Singh
A.V., Singh B., Chaubey K.K., Gupta S.,
Sharma N., Kumar S., Raghava G.P.S. (2013).
Genome Sequence of the Indian Bison
Type Biotype of Mycobacterium avium
subsp. paratuberculosis Strain S5. Journal of
Bacteriology: Genome Announcements 1 :
e00005-13.
98. Singh, S.V., Kumar N., Sohal J.S., Singh A.V.,
Singh P.K., Agrawal N.D., Gupta S., Chaubey
K.K., Rawat K.D., Deb R., Dhama K. (2014).
First mass screening of the human
population to estimate the bio-load of
paratuberculosis in North India. Journal of
Public Health and Epidemiology 6: 20-29.
99. Singh, S.V., Kumar, N., Sohal, J.S., Singh,
A.V., Singh, P. K., Agrawal, N.D., Gupta, S.,
Chaubey, K.K., Rawat, K.D., Deb, R. and
Dhama, K. (2014). First mass screening of the
human population to estimate the bio-load of
paratuberculosis in North India. Journal of
Biological Sciences 14: 237-247.
100. Singh, S.V., Singh A.V., Kumar A., Singh P.K.,
Deb R., Verma A.K., Kumar A., Tiwari R.,
Chakraborty S., Dhama K. (2013). Survival
mechanisms of Mycobacterium avium
subspecies paratuberculosis within host
species and in the environment- A review.
Natural Science. 5: 710-723.
101. Singh, S.V., Singh A.V., Singh P.K., Gupta S.,
Singh H., Singh B., Vinodh Kumar,
Rajendiran A.S., Swain N., Sohal J.S. (2013).
Evaluation of Indigenous vaccine
developed using Indian Bison Type
genotype of Mycobacterium avium
subspecies paratuberculosis strain S5 of
goat origin in a sheep flock endemic for
Johnes disease: A three years trial in India.
World Journal of Vaccines 3 : 52-59.
102. Singh, S.V., Singh P.K., Gupta S., Chaubey
K.K., Singh B., Kumar A., Singh A.V., Kumar
N. (2013). Comparison of microscopy and
blood-PCR for the diagnosis clinical of
Johnes disease in domestic ruminant. Iranian
Journal of Veterinary Research 14: 345-349.
103. Singh, S.V., Singh P.K., Singh A.V., Gupta S.,
Chaubey K.K., Singh B., Kumar A., Srivastav
A., Singh H., Sohal J.S. (2013). Bio-burden and
bio-type profiles of Mycobacterium avium
subspecies paratuberculosis infection in the
domestic livestock population based on mass
screenings using multiple tests. International
Journal of Current Research 5:1897-1901.
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104. Singh, S.V., Singh P.K., Singh A.V., Sohal J.S.,
Kumar N., Chaubey K.K., Gupta S., Kumar
A., Bhatia A.K., Srivastav A.K., Dhama K.
(2014). Bio-load and bio-type profiles of
paratuberculosis infection in the farm and
farmers herds / flocks of domestic livestock:
A 28 years study (1985-2013). Transboundary
and Emerging Diseases 61: 1-13.
105. Singh, S.V., Sohal J.S., Kumar N., Gupta S.,
Chaubey K.K., Rawat K.D., Chakraborty S.,
Tiwari R. and Dhama K. (2014). Recent
approaches in diagnosis and control of
mycobacterial infections with special
reference to mycobacterium avium
subspecies paratuberculosis. Advances in
Animal and Veterinary Sciences 2:1-11.
106. Singh, S.V., Yadav, R. K., Gupta, V. K., Gupta,
S., Chaubey, K. K., Agarwal, N.D. and
Kumar, N. (2014). Co-incidence of Bovine
Johnes disease and Bovine Brucellosis in
Young Bulls of Murrah Breed in their Native
Tract (Rohtak, Haryana, India). Advances in
Animal and Veterinary Sciences 2: 23-25.
107. Sinha, R., Kharche, S. D., Sinha, N. K., Goel,
A. K., Jindal, S. K. and Saraswat, S. (2013).
Effect of cryopreservation on in vitro
maturation and cleavage rate of immature
prepubertal goat oocytes. Indian Journal of
Animal Science 83: 898-902.
108. Sinha, R., Kharche, S. D., Sinha, N. K., Goel,
A. K., Jindal, S. K. and Saraswat, S. (2014).
Effect of vitrification and slow freezing on in-
vitro matured prepubertal goat oocytes.
Indian Journal of Animal Sciences 84: 289-
292.
109. Sohal, J.S., Singh, S.V., Singh, P.K., Singh,
A.V., Kumar, N. (2013). A new marker IS1311
L2 PCR-REA for identification of Indian
Bison Type Mycobacterium avium
subspecies paratuberculosis Indian Journal
of Biotechnology, 12: 204-207.
110. Soren, N.M., Tripathi, M.K., Bhatt, R.S.,
Karim, S.A., (2013). Effect of yeast
supplementation on the growth performance
of Malpura lambs. Tropical Animal Health
and Production 45:547-554.
111. Srivastava, S. and Kumar, S. (2014).
Incorporation of ascorbic acid, caffeine and
chloroquine diphosphate in the dilutor
improves structural and functional status of
frozen semen. OALib. 1: 1-12.
112. Tiwari, R, Chakraborty S, Saminathan M,
Dhama K & Singh SV. (2014). Ashwagandha
(Withania somnifera): Role in Safeguarding
Health, Immunomodulatory Effects,
Combating Infections and Therapeutic
Applications: A Review. Journal of Biological
Sciences, 14 : 77-94.
113. Tiwari, R., Chakraborty S., Dhama K.,
Rajagunalan, S., Singh S.V. (2013). Antibiotic
resistance - An emerging health problem:
Causes, worries, challenges and solutions A
review. International Journal of Current
Research, 5: 1880-1892.
114. Tiwari, R., Verma A.K., Chakraborty S.,
Dhama K and Singh S.V. (2014). Neem
(Azadirachta indica) and its Potential for
Safeguarding Health of Animals and
Humans: A Review. Journal of Biological
Sciences . 14 : 110-123.
115. Tripathi, M.K., Chaturvedi, Deepika.,
Jakhmola, R.C. and Karim, S.A. (2013). Effect
of graded levels of Coriander (Coriander
stivum L.) straw powder inclusion on wheat
straw fermentation and gas production
characteristics in-vitro. Range Management
and Agroforestry 34 : 201-214.
116. Tripathi, M.K., Raghuvansi, S.K.S., Mondal,
D.,Karim S.A., (2014). Effect of Bt-cottonseed
meal feeding on performance, fermentation,
ciliate protozoa population and microbial
hydrolytic enzymes in lambs. African Journal
of Biotechnology.13: 509-522.
117. Tripathi, P., Dutta, T.K., Tripathi, M.K.,
Chaudahry, U.B., Kumar, R. (2014).
Preparation of complete feed pellet from
monsoon herbages (Cenchrus ciliaris,
Dactylotennium aegypticum and Tephorosia
purpurea) and its utilization in kids. Indian
Journal of Small Ruminants. 20(1): 31-36.
118. Vaswani. S., Kumar, Ravindra, Kumar V.,
Roy. D. , and Kumar. M., (2013). In vitro
evaluation of wheat straw varieties for
chemical composition, gas production and
digestibility. Indian Journal of Animal
Research 47 (6) 555-557.
119. Verma, A. K., Rajkumar, V. Rajkumar,
Banerjee, Rituparna Banerjee, Biswas, S.
Biswas and Das, Arun K. Das. (2013). Guava
(Psidiumguajava L.) powder as an
antioxidant dietary fibre in sheep meat
nuggets. Asian Australasian Journal of
Animal Sciences. 26(6): 886-895.
120. Verma, A.K., Banerjee, R. and Sharma, B.D.
(2013). Quality characteristics of low fat
chicken nuggets: effect of salt substitute
blend and pea hull flour. Journal of Food
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Science and Technology:DOI 10.1007/s13197-
013-1218-1.
121. Weber, C., Hametner, C., Tuchscherer, A.,
Losand, B., Kanitz, E., Otten, W., Singh, S. P.,
Bruckmaier, R. M., Becker, F., Kanitz, W. and
Hammon, H. M. (2013). Variation in fat
mobilization during early lactation in high
yielding dairy cows affect feed intake, body
condition as well as glucose and lipid
metabolism. Journal of Dairy Science 96:165
180.
122. Yadav, P., Kharche, S. D., Goel, A. K., Jindal,
S. K. and Goel, P. (2013). Assessment of
nuclear maturation and subsequent in vitro
embryo development of caprine oocytes with
different supplementation in maturation
medium.. Indian Journal of Animal Science
83: 1048-1052.

Lead / Invited Papers
1. Dass, Gopal (2013). Sheep production in
India with special reference to
Muzaffarnagari sheep. State Level Seminar
on Challenges and strategies for
conservation of small ruminants in India at
DUVASU, Mathura, U.P. on Oct 9-10, 2013,
pp. 14-24.
2. Dass, Gopal, P. K. Rout and S. K. Singh
(2014). Goat genetic resources in India and
future strategies for its genetic improvement
and conservation. National Seminar &
Annual Conference on Sheep and goat
biodiversity and breeding Policies Issues
and Perspectives held at Shirwal,
Maharashtra on February 21-22, 2014, pp.
217-229.
3. Dixit A.K., B.Rai, M.K.Singh and
N.Ramachandran (2014. Goat production
Scenario in India : Opportunities and
challenges.Lead paper in National seminar
on New Dimensional approaches for
livestock productivity and profitability
enhancement under era of climate change
held at CVS,Anand (Gujrat) during 28-30
January, 2014, pp 317-324.
4. Dixit, A.K. (2013). Economics of Goat
Farming in India. In: Training Manual on
Nutrition, Management and Prevention of
Goat Diseases for Optimum Productivity
held during 10-16 April, 2013 for Veterinary
Officers of State AHD of UP and UK under
Trainers Training Programme (Skill
Development) Sponsored by Department of
DADF, Ministry of Agriculture, Government
of India.
5. Dixit, A.K. and Birthal,P.S. (2013). Positive
Environmental Externalities of Livestock in
Mixed Farming Systems of India.
Agricultural Economics Research Review.
26(3):21-30.
6. Dixit, A.K., M.K. Singh and B. Rai (2014) Goat
production and marketing in India. Lead
paper in National seminar on Sheep and goat
biodiversity and breeding policies issues and
perspective held at Mahabaleshwar (Satara)
M.H. on 21-22 Feb, 2014 pp 81-92.
7. Dixit, A.K.,Singh,M.K. and Rai,B. (2014).
Goat Production and marketing in India.
Published as lead paper in Souvenir of
Annual Conference on Sheep and Goat
Biodiversity and Breeding Policies-Issues
and Perspectives organized by Department
of ARGO,KNPC of Veterinary Sciences,
Shirwal and Indian Society for Sheep and
Goat Production and Utilization on February
21-22, 2014.
8. Goel, A.K. and Kharche S. D. (2014).
Emerging Reproductive Biotechnologies in
Small Ruminants. In: Abstracts & Souvenir,
National Seminar on Sheep and Goat
Biodiversity and Breeding Policies-Issues
and Perspective held at Department of
ARGO, K N Patil College of Veterinary
Science, Shirwal, MAFSU, Nagpur (MS State)
on Feb 21-22 2014, pp. 369-384.
9. Kharche, S.D., S.K .Jindal, Satish Kumar,
A.K. Goel and Ravi Ranjan (2014). Recent
Advances in augmentation of reproduction
in goats Proceeding National Seminar and
Annual Conference of ISSGPU on Sheep and
Goat Diversity and Breeding Policies: Issues
and perspectives. 21-22nd Feb. 2014, KNPVC,
Shiervel, Maharashtra.
10. Kharche, S.D. (2014). Oestrous
synchronization in goats. Lecture delivered
at Krantisinh Nana Patil College of
Veterinary Science, Shirwal (Satara).
11. Kharche, S.D. and Agarwal, S.K. (2013).
Assisted reproductive technologies for
enhancing goat production. Interactive
meeting on Prospects in improving
production, Marketing and value addition of
carpet wool, Arid Region Campus of CSWRI
Avikanagar at Bikaner, December 31 2013,
pp. 28-35.
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88
12. Kharche, S.D., Gangwar, Chetna and
Agarwal, S.K. (2014). Animal Cloning:
Technological Development for future
application. National Symposium on
Frontier Reproductive Biotechnologies for
enhancing Animal fertility and fecundity
global perspective and XXIX annual
convention of Indian society for study of
animal reproduction organized by
Department of ARGO at MAFSU Nagpur,
January 8-10, 2014, pp. 297-311.
13. Kharche, S.D., Gangwar, Chetna, Ranjan, R.
and Agarwal, S.K. (2014). Reproductive
technologies for enhancing animal
production. 2nd Annual meeting of society
of veterinary science and Biotechnology and
national seminar on Biotechnologies
approaches to challenges in animal health
and production, U.P. Pt. Deen Dayal
Sansthan, Mathura, March 6-7, 2014 pp 72-
79.
14. Kharche, S.D., Goel, A.K., Jindal, S.K.,
Gangwar, Chetna, Ranjan, R., Saraswat, S.,
Pathak, Juhi, Agarwal, Surbhi, Sikarwar,
A.K.S., Rout, P.K., Vijh, R.K., Malakar, D.,
Bag, S. and Agarwal, S.K. (2014).
Developmental potency of parthenogenetic
goat embryos following in vivo transfer in
capra hirus. International conference on
reproductive health: Issue and strategies
under charging climate scenario held at
IVRI, Izatnagar Bareilly, 6-8 February, pp 71.

15. Kharche, S.D., Goel, A.K., Jindal, S.K.,
Gangwar, Chetna, Ranjan, R., Saraswat, S.,
Pathak, Juhi, Agarwal, Surbhi, Sikarwar,
A.K.S., Rout, P.K., Vijh, R.K., Malakar, D.,
Bag, S. and Agarwal, S.K. (2014).
Developmental potency of parthenogenetic
goat embryos following in vivo transfer in
capra hirus. International conference on
reproductive health: Issue and strategies
under charging climate scenario held at
IVRI, Izatnagar Bareilly, February 6-8, 2014,
pp 71.
16. Kharche, S.D., Ranjan, R. and Agarwal, S.K.
(2014). Parthenogenesis: Present Status and
Future Prospect. XXII Annual Conference of
Society of Animal Physilologist of India and
National Symposium on Physiological and
Nutri-genomics interventions to augment
food security and Animal Welfare organized
by Department of Veterinary Physiology at
U.P. Pt. Deen Dayal Upadhyaya Pashu
Chikitsa Vigyan Vishwavidyalya evan Go-
Anusandhan Sansthan, Mathura, November
19-21, 2013, pp 72-84.
17. Kumar, Ashok ( 2014) Technologies of CIRG
for enhancing goat productivity in Asia and
Africa . Lecture delivered to Senior Officers
of Asia and Africa organised by IIM Noida ,
26-3-14.
18. Kumar, Ashok (2014).Goat Health and
management of Goatery Unit. Deliberation
and interaction at State specific farmer
Scientific interaction during Krishi Vasant
2014 at National Agriculture fair cum
exhibition at Nagpur during February9-13,
2014. Hall -1, Session IV, Feb 10, 2014.
19. Kumar, Ashok, V K Gupta and Ravindra
kumar (2014). Scientific and economic goat
farming in Punjab. In Technical session
Rearing of small ruminant Progressive
Punjab summit-2014 organised by Govt of
Punjab, Chandigarh on 18-2-14
20. Kumar, Ashok, V K Gupta, R V S Pawaiya, K
Gururaj, Shivasharanappa N (2014).
Neonatal health and survival in small
ruminants. National seminar and annual
conference on Sheep and goat biodiversity
and breeding policies Issues and
perspective held at MAFSU, KNP college of
Vet Sci , Shirwal ( Satara ) Maharastra, pp.
497-503.
21. Kumar, Ashok, V.K.Gupta, K.Gururaj and
Nitika Sharma (2014). Molecular markers:
their role in herbal medicine. In:
Compendium of National Seminar on
Biotechnological approaches to challenges in
animal health and production organized by
Society of Veterinary Science and
Biotechnology at DUVASU, Mathura, 6-7th
March, 2014,pp. 41-45.
22. Kumar, N. (2013). Pox virus infections in
small ruminants: their diagnosis and control
in CAFT training course on diagnosis and
control of infectious diseases of small
ruminants being organised by LLR
University of Veterinary and Animal
Sciences, Hisar.
23. Kumar, N. (2014). Antiviral medication of
livestock. Where we are? In, second Annual
meeting of the Society of Veterinary Science
and Biotechnology and National Seminar on
Biotechnological approach to challenges in
animal health and production, held at
Veterinary University Mathura from 6-7
March 2014.
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89
24. Kumar, Satish, Chetna Gangwar, Ravi Ranjan
(2014). Advances in reduction of cryo-
damages to sperm cells. Proceeding National
Seminar and Annual Conference og ISSGPU
on Sheep and Goat Diversity and Breeding
Policies: Issues and perspectives. 21-22nd
Feb. 2014, KNPVC, Shiervel, Maharashtra.
Pp.335-345.
25. Mishra, A. K. Sharma N. and Gururaj K.
(2014). Phage therapy: An alternative to
antibiotics. In: Compendium of national
seminar on Sheep and goat biodiversity and
breeding policies: Issues and prospective
held at Shirwal, Maharashtra on Feb 21-22,
2014. LP-5-2, pp.491-496.
26. Rai, B., Ravindra Kumar, Ramachandran N.,
Dixit H.and Rai R.B. (2014). Village based
goat feeding system in eastern part of U.P.
Opportunities and challenges. in National
seminar on New Dimensional approaches for
held at CVS,Anand (Gujrat) 28-30. January,
2014 pp 153.
27. Ramachandran, N., M.K.Tripathi, B.Rai,
S.K.Singh,V. Kumar and S.K.Jindal (2014)
Effect of castration on growth,feed intake and
feed efficiency in Barbari kids. Opportunities
and challenges in National seminar on New
Dimensional approaches for livestock
productivity and profitability enhancement
under era of climate change held at
CVS,Anand (Gujrat) pp. 28.
28. Rout, P.K. (2013) Genetic diversity in small
ruminants with special reference to
adaptation and disease resistance. Seminar
onChallenges and strategies for
conservation of small ruminants in India
October9-10, 2013, College of Veterinary and
Animal Sciences, DUVASU, pp 47-51.
29. Rout, P.K. (2014).Genetics of disease
resistance: A sustainable strategy for
improving production efficiency in livestock
and poultry, pp101-107, National Seminar on
animal health and production, March 6-7,
2014, DUVASU, Mathura
30. Rout, P.K.(2014).Genetics of laboratory
animals and their welfare,Pp-36, National
symposium on animals and alternatives in
life science research(NSAALR), February 16-
18,Department of Zoology, BHU, Varanasi,
India.
31. Singh, M. K., S K Singh and M S Dige. (2014).
Goat Improvement Programmes in India: An
Over View. Published in Souvenir cum lead
papers of National Seminar and Annual
Conference on Sheep and Goat Biodiversity
and Breeding Policies_ Issues and
Perspective, February 21-22, 2014 held at
Krantisinh Nana Patil College of Veterinary
Science, Shirwal (Satara), MA&FSU, Nagpur,
Maharshtra. Pp:177-185
32. Singh, M.K., B.Rai, A.K.Dixit, M.S.Dighe,
N.Ramachandran and S.K. Singh (2014)
Management practices of goats in
Bundelkhand region. Iin National seminar on
Sheep and goat biodiversity and breeding
policies issues and perspective held at
Mahabaleshwar (Satara) M.H. on 21-22 Feb,
2014 pp 186.
33. Singh, S,K. (2013). Conservation Strategies
for Genetic resources of Small ruminants at
NASC Complex, New Delhi on 10-01-14.
34. Singh, S.K. (2013). Goat Farming: A Viable
Enterprise for Smallholders and Landless
People. Paper presented at National
Conference on KVK, held at Bangluru, Oct
23-25, 2013.
35. Singh, S.V. (2013) National symposium and
XXVII Annual Convention of IAVMI on
Productivity enhancement through
improved animal health and nutrition, 13-15
December, 2013. Organized by Department
of Animal husbandary, Lucknow and Indian
Association of Veterinary Microbiologists,
Immunologists and Specialists in infectious
diseases.
36. Singh, S.V. (2013) Status Paper on Bio-
burden and bio-type profiles of
paratuberculosis infection in the farm and
farmers herds / flocks of domestic livestock
in India: A 28 years of study (1985-2013).
37. Singh, S.V. (2013) VIROCON-2013, Asia-
Pacific Congress of Virology, 17-20 December
2013, Organized by Amity University, Noida,
New Delhi. Oral presentation.
38. Singh, S.V. (2013) XX Annual convention of
Indian Society for Veterinary Immunology
and Biotechnology & National Symposium
on "Emerging Challenges & Opportunities in
Veterinary Immunology & Biotechnology for
Improved Animal Health & Productivity"
November 11-13, 2013. Dr. G.C. Negi College
of Veterinary & Animal Sciences, CSKHPKV,
Palampur-176062, Himachal Pradesh.
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Popular articles
1. Bhardwaj M, Abhishek, Mishra A K, Kumar
N and Karthik K, 2013. Cryptococcosis: An
opportunistic disease. Livestock Line 7 (5):35-
356
2. Bhusan, S. (2013). Goat Farm: Advantage of
data recording (hindi). Ajamukh, Ank-27, p-
3.
3. Bhusan, S. (2013). Utility of data recording in
genetic improvement of goats. CIRG News, .
VIII, p-6.
4. Das, A.K., Verma, A. K. and Rajkumar, V.
(2014). Goat meat based value added
products. Goat news. Vol 8 (Jan-June). P- 11.
5. Dixit, A.K., and Mohan, B., (2013).
Compositional Change in Goat Population in
India. CIRG, News, ume IX, July-December,
2013, PP: 7.
6. Dixit, A.K., Mohan, B., Singh, K. and Yadav,
U.C. (2013). Marketing Strategies in Goats for
Better Price Discovery. CIRG, News ume-VIII
January- June 2013, pp: 7.
7. Dixit, A.K., Mohan, B., Singh, K. and Yadav,
U.C. (2013).
BakariEvamUnkeUtpadonkaVipran.Samasy-
ayan EvamSujhav. Ajamukh, No. 27, Jan.-
June, 2013, PP: 6-7.
8. Gangwar, Chetna, Kumar Ravindra and
Ranjan, R. (2014) Bakriyon ke uttam prajnan
hetu khanij tatwo ki mahatta. Ajamukh 1, pp
2
9. Gangwar, Chetna, R.Ranjan and S.D.Kharche
( 2014) Himikrit virya dwara bakriyon me
kritrim garbhadhan, Kheti January, 17-18.
10. Gangwar, Chetna, Ranjan, R. and Jindal, S.K.
(2013) Fertility associated proteins can be
used as fertility marker ? CIRG News VIII. Pp
10.
11. Goel, A.K. (2013) Bakri utpadan mein janan
techniques ka yogdaan. Vishwa ki pragati me
vigyan tatha prodyogiki ka yogdan, 05-07
Dec, 2013, Desidoc, Delhi
12. Gopal Dass (2013). Muzaffarnagari Ek
shreshtha mans utpadal bhed nasl. Ajamukh
(Hindi magazine) of CIRG, 28th issue, July-
December, 2013, page: 8.
13. Gururaj K (2013).Next generation viral
vaccines against goats: A backdoor approach.
CIRG News 9: 3
14. Kharche, S D., Goel A K and Jindal S K,
(2013). Development of parthenote in goats-
First report. CIRG News. VIII. p 4.
15. Kharche, S D., Jindal S K, Priyadharsini, R,
Satish Kumar, Goel A K, Ramachandran N.
2013. AI technology using frozen semen in
goats. CIRG News. VIII. p 10.
16. Kumar Ravindra, Tripathi P. Chaudhary,
U.B. and Tripathi, M. K. (2013)Azolla-Bakri
ahar ke liye vaikalpik sroth Ajamukh pp8 (28)
July-Dec, 2013.
17. Kumar Vijay, Braj Mohan, Singh, K. and
Dixit, A.K. (2013). Positive trends of exposure
visits. CIRG News IX: 8.
18. Kumar Vijay, Braj Mohan, Dixit, A.K., Singh,
K., Prasad, D., Yadav, U.C. and Gautam,
S.C.L. (2013). CIRG prakshetra bharman ke
parti sakaramatak rughan. Ajamukh 28:7.
19. Mishra A. K. Sharma N. and Paul S. (2013).
Bacteriophage endolysin: A novel
therapeutic agent. Ajamukh 27:8-8 (Article in
Hindi)
20. Mukherjee R, De U K and Sharma N. (2013).
Treatment and control of mastitis with non-
antibiotic agents in lactating dairy animals.
Livestockline, 7 (2): 35-37.
21. Paul S, Sharma DK, Boral R, Mishra AK,
Shivsharanappa N, Banerjee PS and Pawaiya
RVS (2014). Cryptosporidiosis in goats; a
review. Adv. Anim. Vet. Sci.2 (3S): 49 54.
22. Paul, Souvik, Geetika Gupta, A K Mishra,
Nitika Sharma and Shivasharanappa N
(2013). Cryptosporiodium: The hidden
neonatal pathogen. CIRG Newsletter, 9: 6-7.
23. Paul, Souvik, Shivasharanappa N, Nitika
Sharma and A K Mishra (2013). Body
condition scoring (BCS) in goats- A step
forward to commercial goat farming. CIRG
Newsletter, 8: 2.
24. Rai B. (2013). Bakri palan se ajeevika
suraksha. Ajamukh. 27. P9.
25. Rajkumar, V., Verma, A. K. and Das, A.K.
(2014). Efficient utilization of Goat Slaughter
by-product: Blood. Goat news. Vol 8 (Jan-
June). P- 5.
26. Ramachandran N, Singh M K, Dixit A K and
Singh R. (2013). Design and popularization of
low cost shelter models for goats in
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disadvantageous districts of Bundelkhand
region. CIRG News. VIII. p 12.
27. Ramachandran N, Tripathi, M K, Rai B, Jindal
S K, Dixit A K and Souvik Paul (2013).
Designing of adjusted slatted floor for goats.
ISSGPU News Letter. p4.
28. Ranjan R and Kahrche, S D. (2013). Tetraploid
complementation assay: Is live birth possible
from parthenogenetic embryonic stem cell?
CIRG News. VIII. p 10.
29. Ranjan R, Gangwar Chetna and
Ramachandran N. (2013). Beta Defensins:
Can protect sperms from Immunological
aggression by female. CIRG News. VIII. p 9.
30. Sharma N, Mishra A K, Chaturvedi V and
Paul S. (2013). Pratham amashay ki amalta (
ruminal acidosis). Ajamukh, July-Dec,
volume 28, Page 6.
31. Sharma N, Rout P K, Kumar A and
Shivasharanappa N.(2013). Role of women in
goat husbandry. C.I.R.G Newsletter, July-
Dec, 2013, volume IX, Page 4-5.
32. Sharma N, Shivasharanappa N and Mishra A
K (2013). Goats in Indian Mythology. C.I.R.G
Newsletter, Jan-June, 2013, volume VIII, Page
4.
33. Sharma, Nitika, Vinay Chaturvedi and
M.K.Singh. (2013). Atresia ani. Ajamukh, Jan-
June, volume 27, Page 5.
34. Singh M K, Dixit A K, Kumar N,
Ramachandran N, Yadav J S, Singh A K,
Singh R, Singh P. (2014). Goat- for Nutritional
and livelihood security in Bundelkhand
region. Indian Farming 63(10): 50-54.
35. Singh, M.K, N.Kumar, A.K.Dixit, S.K.Singh
and B.Rai (2013) Bundelkhand kshetra mein
bakari palan ke sutra Folder published by
CIRG under NAIP Com-3 project.
36. Tripathi Manoj Kumar, Tripathi Prabhat,
Manoj Kumar Singh, Ajay Kumar Rai and
Styendra Kumar Singh. (2013). Buldel Khand
Main Pashuao Ki ekikrat Poshan Taknikiya.
Central Institute for Resaerch on goats,
Makhdoom, Farah, Mathura.

Radio Talk /TV Programme
1. Agarwal S.K. (2013). Recent Scientific
achievements of Central Institute for
Research on Goats. All India Radio, Mathura
Recording on 26.8.2013 and broadcasted on
28.8.2013.
2. Agarwal S.K. ( 2013). T.V. interview at D.D.
National, New Delhi on 22 July, 2013
highlighting major activities and
achievement of the institute for enhancing
goat production. It was telecasted on 24th
and 29th July, 2013 under Krishi Darashan
Programme on DD National New Delhi.
3. Agarwal S.K.(2014). Phone in LIVE
PROGRAMME in Krishi Darshan on the
subject Bakri Palan-labhkari Vyavsyaya
recorded and telecasted live at 5-6 PM on
13.3.2014 by D.D. National, New Delhi.
4. Bhushan, Saket (2013). Breeds of goats for
Braj area and their Characteristics on
14.08.13 from All India Radio, Mathura (UP).
5. Dixit, A.K. (2013). (Aaj Ki Baat) on Bakario ka
Vipadan Evam Bakri Vikas Sambandhit
Yojnaye on 25.10.2013 at Akashvani Mathura.
6. Goel, A.K. (2013). Aaj ki baat : Aaj ke parivesh
me bakri palan ka mehtab, All Indian Radio,
Mathura 10.5.2013
7. Goel, A.K. (2013). Aaj ki Baat: Jaade ke
mausam me bakri palako ko sujhab. All India
Radio, Mathura 25.12.2013
8. Kumar, Ashok (2014). Pashu palan evam
Pashu Rog ke Samadhan Live programme
in Hello Akashwani at All India Radio
Mathura on 24-3-14
9. Kumar, Ashok Kumar (2014). Pashu palan
evam Pashu Rog Live programme in Hello
Akashwani at All India Radio Mathura on 22-
10-2013
10. Kumar, Naveen (2013). Barsat ke Mausam
mein Bakri palako dwara barti jani wali
savdhaniya, 7th August 2013, Akashwani,
Prasar Bharati, Mathura
11. Sharma, R.B.(2013). Bakri ke doodh aur uske
utpaad ki maang aur marketing All India
Radio, Mathura 19.08.2013

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Book /Bulletins/Manual/Chapter/Compendium
1. Abhishek, Mishra A K, Gupta C and Agrawal
R K (2014). Dermatophytosis: Trichophytosis.
In: Bhushan B, Gaur G K, Sharma D,
Mendiratta S K, Verma A K, Pawde A M,
Singh M, Khan T A, Patel B H M, Pandey H O
AND Panigrahi M (Eds.). Livestock
Management. Indian Veterinary Research
Institute, Izatnagar. pp. 298-300
2. Agarwal S.K. and Singh S.K. (2013) Basics of
Animal Reproduction. In: Handbook of
Animal Husbandry. Published by
Directorate of Knowledge Management in
Agriculture, Indian Council of Agricultural
Research, New Delhi Fourth Revised and
Enlarged Edition
3. Chaturvedi V, Sharma N and Paul S. (2013).
Health parameters in Goats. In: Training
Manual on Nutrition, Management and
Prevention of Goat Diseases for Optimum
Productivity held at CIRG, Makhdoom,
Farah, Mathura (UP) on April 10-16/Oct 21-
29, 2013 for Veterinary Officers of State
Animal Husbandry Department of Uttar
Pradesh and Uttarakhand under Under
Development) sponsored by DADF,
Ministry of Agriculture, Government of
India. Chapter 11, Page no. 60-64.
4. Chaudhary, U.B., Tripathi, M.K., Tripathi,
Prabhat and Kumar, R. (2014). Nutritional
strategy to harness the productivity of goat
diversity. Proc. National Seminar on Sheep
and Goat biodiversity and breeding policies-
issues and perspectives, February 21-22,
KNP College of Veterinary Science (MAFSU),
Shirwal, Satara, Maharashtra. 164-172.
5. Dass, Gopal (2013). Chayan prakriya dwara
Muzaffarnagari bhedon ke utpadan evam
janan gudon men anuvanshik sudhar.
Vaigyanik Anusandhan tatha Vikas, DRDO,
Raksha Mantralaya Prakashan, Year 2013,
page 158-162. (In Hindi).
6. Gupta, V K, and Ashok Kumar. Diagnosis of
Brucellosis in Goats. Training Manual on
Nutrition, Management and Prevention of
Goat Diseases for Optimum Productivity
held during 10-16 April, 2013 for Veterinary
of IndiaPage no. 88-97.
7. Gupta, V K, Shivasharanappa N and Ashok
Kumar. Vaccines and Vaccination in Goats.
Training Manual on Nutrition,
Management and Prevention of Goat
Diseases for Optimum Productivity held
during 10-16 April, 2013 for Veterinary
of India Page no 141-146.
8. Kharche S D. (2013). Embryo Transfer
Technology in Farm Animals. In: Hand book
on Animal Husbandry, ICAR Publication pp.
1042-1066.
9. Kumar, Ashok, VK Gupta, RVS Pawaiya,
Ravindra Kumar and PK Rout. (2013).
Editors. Training Manual on Nutrition,
Diseases for Optimum Productivity. 2013;
Published by Prioritization, Monitoring and
Evaluation Cell, CIRG, Mahdoom, Mathura
(U.P.); 153 pages.
10. Kumar Ravindra and Tripathi, P. (2013)
Nutraceuticals in Livestock practices. Pp
265-272. In Book : Livestock production and
Management Recent Trends and Future
prospects. New India Publishing Agency,
New Delhi
11. Manjunatha Reddy, G.B., P.K. Rout and
Shivasharanappa N (2013). Bioinformatics in
livestock sector. In: Advances in Livestock
Production and Management, Pages 17
Edited by: Sunil Kumar, Birendra Kumar
Mishra and Manish Kumar, 2013, Jaya
Publishing House, Delhi, India.
12. Mishra, A K, Kumar A, Sharma N and
Shivasharanappa N (2013). Bacterial Diseases
of Goats. In: Training Manual on Nutrition,
Diseases for Optimum Productivity held at
CIRG, Makhdoom, Farah, Mathura (UP) on
April 10-16/Oct 21- 29, 2013 for Veterinary
Officers of State Animal Husbandry
Department of Uttar Pradesh and
Uttarakhand under Under Trainers
Training Programme (Skill Development)
sponsored by DADF, Ministry of Agriculture,
Government of India.Chapter 12, pp. 65-71.
13. Paul, Souvik (2013). Molecular basis of
acaricide resistance in Indian ticks. In:
Compendium on Assessment of resistance in
ticks to chemical acaricides and processing of
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plant based anti-tick products. Under NAIP
sponsored Joint Workshop, pp. 20-22.
14. Paul, Souvik, Shivasharanappa N, Sharma,
Nitika and Sharma, D.K. (2013). Collection
and examination of ecto-parasites in goats.
In: Training Manual on Nutrition,
management and prevention of goat diseases
for optimum productivity for veterinary
officers of UP and UK under Trainers
Training Programme (Skill Development)
sponsored by Department of DADF, Ministry
of Agriculture, Government of India held
during April 10-16, 2013,pp. 106-110.
15. Sharma N, Mukherjee R and Mishra A K.
(2013). Preventive and Therapeutic
Modalities for Control of Bovine Mastitis.
Alina Covali and Aurora Jian (Eds.). Lambert
Academic Publishing, Saarbrucken,
Germany. ISBN: 978-3-659-36414-3.
16. Sharma N, Shivasharanappa N, Mishra A K,
Chaturvedi V and Kumar S. 2013. Control of
important viral diseases of goats. In: Training
Productivity held at CIRG, Makhdoom,
Farah, Mathura (UP) on April 10-16/Oct 21-
29, 2013 for Veterinary Officers of State
Animal Husbandry Department of Uttar
Pradesh and Uttarakhand under Under
Development) sponsored by DADF,
Ministry of Agriculture, Government of
India. Chapter 13, Page no. 72-79.
17. Shivasharanappa N., Nitika Sharma, A.K.
Mishra and R.V.S. Pawaiya (2013). Collection
and dispatch of morbid materials for disease
investigations. Page no. 111-116. Training
Productivity held during 10-16 April, 2013
for Veterinary Officers of State AHD of UP
and UK under Trainers Training Programme
(Skill Development) Sponsored by
Department of DADF, Ministry of
Agriculture, Government of India.
18. Tripathi Manoj Kumar, Tripathi Prabhat,
Manoj Kumar Singh, Ajay Kumar Rai and
Styendra Kumar Singh. (2013). Buldel Khand
Main Pashuao Ki ekikrat Poshan Taknikiya.
Central Institute for Resaerch on goats,
Makhdoom, Farah, Mathura. Central
Institute For Research on Goats, Makhdoom,
Farah, Mathura
19. Tripathi Prabhat, Dutta, Tapas Kumar,
Tripathi, Manoj Kumar, Chaudhary, U.B. and
Kumar, Ravindra (2013). Varsha Adharit
Charagahon Ke Vibhinn Praroopo ka Bakri
Palan Hetu Mulayankan In:Vartman
Vaigyanik Anushandhan, published by
DRDO, DESIDOCK, Metcalf House
Delhi.136-139.
20. Tripathi, P. and Kumar Ravindra. 2013.
Fodder production for Livestock through
silvipasture system. Pp 39-54. In Book:
Advances in Livestock production and
Management. Jaya publishing House, New
Delhi.
21. Verma, A.K. and Banerjee, R. (2013). Dietary
fiber in meat products: Functional and
bioactive ingredients. In: Betancur-Ancona,
D., Chel-Guerrero, L., Segura-Campos, M.R.
(Eds.), Dietary fiber: sources, properties and
their relationship to health, Nova Science
Publishers, New York, pp. 63-84.
22. Verma, A.K., Banerjee, R., Dwivedi, H.P.,
Juneja, V.K. (2014). Bacteriocins: Potential in
Food Preservation. In: Batt, C.A., Tortorello,
M.L. (Eds.), Encyclopedia of Food
Microbiology, vol 1. Elsevier Ltd, Academic
Press, pp. 180186.

Papers presented in National and Internationl conferences
1. Bhusan, S. (2013). Genetic improvement of
production and reproduction traits in goats
of Jakhrana breed (in hindi). Proceeding of
National Seminar on New technology of
agricultural and allied sciences:
Achievements & challenges (in Hindi
language) held from- 11-13 December 2013
at Central Institute of Fisheries Education,
(I.C.A.R.) Versova, Mumbai.
2. Bhusan, S. (2014). Studies of some non-
genetic factors and their impact on milk
production of Jakhrana goats XI National
Symposium, Harmonizing phenomics and
genomics for sustainable management of
livestock for upliftment of rural masses
organized by SOCDAB, NBAGR, Karnal held
on 6-7 February, 2014 at NBAGR, Karnal.
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94
3. Bhusan, S. and Gopal Dass (2014). Effect of
non genetic factors on growth efficiency of
Jakhrana kids. Published in the compendium
of National Seminar & Annual Conference on
Policies Issues and Perspectives held at
Shirwal, Maharashtra from 21-22 February,
2014, Page No. 547.
4. Chaturvedi, V., Sharma, N., Tiwari, H. A.,
Kumar, A., Shivasharanappa, N., Mishra, A.
K., Paul, S., Singh, S.V., Dass, G. and Singh,
MK, (2014). Surgical repair of atresia ani in
new-born kids and lambs. In: Compendium
of national seminar on Sheep and goat
biodiversity and breeding policies: Issues
and prospective held at Shirwal,
Maharashtra on Feb 21-22, 2014. Abstract AB-
5-11, pp. 511.
5. Dass, Gopal (2013). Evaluation of wool
production and quality of Muzaffarnagari
sheep. Published in the compendium of
Interactive Meeting on Prospects in
improving production, marketing and value
addition of carpet wool organized by
ISSGPU, CSWRI & CIRG at ARC of CSWRI,
Bikaner on 31 December, 2013, Page No.52.
6. Dass, Gopal (2014). Evaluation of production
and reproduction traits of Muzaffarnagari
sheep. Published in the compendium of
National Symposium on Harmonizing
phenomics and genomics for sustainable
management of livestock for upliftment of
rural masses held at NBAGR, Karnal from
February 6-7, 2014, Page No.146.
7. Dixit, A. K., Rai, B., Singh, M. K. and
Ramachandran, N. (2014). Goat production
scenario in India: Opportunities and
Challenges. In: Abstract No. F-50 National
Seminar on New Dimensional Approaches
for Livestock Productivity and Profitability
Enhancement under Era of Climate Change
& XXI Annual Convention of ISAPM held at
AAU, Anand, Gujarat, January 28-30, 2014,
pp 270.
8. Dixit, A.K., Mohan, B., Singh, K., Kumar, V.,
Yadav, U.C. and Gautam, S.C.L. (2014).
Economics of Goat Production and
Constraint Analysis in Semi-Arid Zone of
Uttar Pradesh. Abstract Printed in
Compandium of National Seminar & Annual
Conference on Sheep and Goat Biodiversity
and Breeding Policies Issues and
Perspective organized by ISSGPU &
KNPCVS, Shirwal, Satara, Mohavashtra at
Mohabaleshwar, Maharashtra on February
21-22, 2014, PP: 186.
9. Dixit, A.K.,M.K.Singh.,B.S. Reddy and
Narendra Singh (2013). Shrinking Pasture
and Grazing Lands: A Concern to Small
Livestock Holders. Agricultural Economics
Research Review, 26 (Conference Number)
2013 pp 232.
10. Gangwar, Chetna, Prasad, S., Prasad, J.K.,
Ranjan, R., Kharche, S.D., Jindal, S.K. and
Agarwal, S.K. (2014). Modulation of
endocrine profile in buffaloes by using
antiluteolytic agent. International
conference on reproductive health: Issue and
strategies under charging climate scenario
held at IVRI, Izatnagar Bareilly, 6-8 February,
pp 211.
11. Gangwar, Chetna, Rajan, R., Kharche, S.D.,
Kumar, Satish, Goel, A.K., Ramachandran,
N., Jindal, S.K. and Agarwal, S.K. (2013).
Optimization of ethylene diamine tetra acetic
acid concentration in Buck semen diluter.
XXII Annual Conference of Society of
Animal Physiologists of India (SAPI) &
Nutri-genomic Interventions to Augment
food security and Animal welfare held at
Anusandhan Sansthan, Mathura, 19-21
November, 2013, pp 35.
12. Gangwar, Chetna, Ranjan, R., Kharche S. D.,
Kumar, S., Goel, A.K., Ramachandran, N. and
Jindal, S.K. (2014). Use of chelating agent to
enhance the post thaw quality of bucksemen.
In: Abstract No. AB-4-3 National Seminar on
sheep and goat biodiversity and breeding
policies-issues and perspective & Annual
Convention of ISSGPU held at KNPCVS,
Shirwal (Satara), February 21-22, 2014, pp
457.
13. Gangwar, Chetna, Ranjan, R., Kharche, S.D.,
Kumar, Satish, Goel, A.K., Ramachandran, N.
and Jindal, S.K. (2014). Use of chelating agent
to enhance the post thaw quality of buck
semen. Sheep and Goat Biodiversity and
breeding policies-Issues and perspective and
Annual conference of Indian Society for
sheep & Goat Production, held at Krantisinh
Nana Patil College of Veterinary Science,
Shirwal (Satara), 21-22 February, 2014, pp
230-319.
14. Gangwar, Chetna, Singh, S.M., Saxena, A.,
Kumar V. and Chaudhary, M. (2014). Serum
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95
concentration of calcium, phosphorous and
magnesium in pregnant cow with and
without vaginal prolapse in Mathura Distt.
Anusandhan Sansthan Mathura, 19-21,
November 2013, pp 107.
15. Gururaj K, Gupta VK, Shivasharanappa N,
Mishra A K, Kumar A and Rout PK, 2014.
Sequencing and analysis of TLR-2 gene of
barbari goat a marker for disease resistance.
In: Compendium of national seminar on
policies: Issues and prospective held at
Shirwal, Maharashtra on Feb 21-22, 2014.
Abstract AB-2-2, pp. 260.
16. Hussler, S., Singh,S. P., Laubenthal, L.,
Locher, L., Winkler, J., Meyer, U., Dnicke S.
and Sauerwein, H. (2013). Impact of
increased oxidative stress through excessive
accumulation of adipose tissue on circulating
adiponectin concentrations in dairy cows.
Joint Annual Meeting of American Dairy
Science Association (ADSA)American
Society of Animal Science (ASAS),
Indianapolis, Indiana, USA, 0812 July 2013.
J. Dairy Sci. Vol. 96 (E-Suppl. 1): 114.
17. Kharche, S.D., Jindal, S.K., Goel, A.K.,
Kumar, S., Sonia, Saraswast, Gangwar,
Chetna, Ranjan, R. and Agarwal, S.K. (2014).
Zona free hamster ova penetration test: a
fertility indicator of buck semen. Sheep and
Goat Biodiversity and breeding policies-
Issues and perspective and Annual
conference of Indian Society for sheep & Goat
Production held at Krantisinh Nana Patil
College of Veterinary Science, Shirwal
(Satara) 21-22 February, 2014, pp 456.
18. Kharche, S.D., S.K. Jindal, Goel A.K., Kumar
S, Sonia Saraswat, Chetna Gangwa,r Ravi
Ranjan and S.K.Agarwal (2014). Zona free
Hamster Ova Penetration Test: A fertility
indicator of buck spermatozoa Proceeding
National Seminar and Annual Conference og
ISSGPU on Sheep and Goat Diversity and
Breeding Policies: Issues and perspectives.
21-22nd Feb. 2014, KNPVC, Shiervel,
Maharashtra.
19. Kopp C., Singh, S.P., Sauerwein, H. and
Mielenz, M. (2013). Niacin increases
adiponectin secretion in differentiated
bovine preadipocytes in vitro via G-protein
coupled receptor 109A. Joint Annual
Meeting of American Dairy Science
Association (ADSA)American Society of
Animal Science (ASAS), Indianapolis,
Indiana, USA, July 0812 2013. J. Dairy Sci.
Vol. 96 (E-Suppl. 1): 121.
20. Kumar, Ravindra, Chaudhary, U.B., .Kumar,
A. and Sharma, D.K., Tripathi P and Tripathi,
M. K. (2013) Intake and growth performance
of barbari goats fed with Ailanthus excels
leaves based complete pellet feed. In
proceeding of World Congress on Agro
forestry 2014 held from 10-14 Feb 2014 at
Delhi, India.
21. Mishra A K, Sharma N, Kumar N, Gururaj K,
Shivasharanappa N, Paul S and Chaturvedi
V, 2014 . Isolation and characterization of
bacteriophages virulent to Staphylococcus
aureus associated with dermatitis in goats. In:
Compendium of national seminar on Sheep
and goat biodiversity and breeding policies:
Issues and prospective held at Shirwal,
5-3, pp. 507
22. Mishra AK, Nitika Sharma, Naveen Kumar,
Shivasharanappa N, K Gururaj, Abhishek,
Souvik Paul and Vinay Chaturvedi. 2013.
Prevalence and Etiology of Subclinical
Mastitis in Barbari and Jamunapari goats.
International symposium on Latest trends in
Immunodiagnosis, Immunopathology and
immunomodulation and VI Convention of
Society for Immunology and
Immunopathology at Rajasthan University of
Veterinary and Animal sciences from Dec 22-
24th, 2013. Journal of Immunology and
Immunopathology, Special Issue, Vol. 15(1):
Jan-June 2013. Page no 142.
23. Mohan, B., Dixit, A.K., Singh, K. and Kumar,
V. (2013). Bakari Palakon ka Samajik-Arthik
Estar Evam Kathinaiyan. Presented in Hindi
Shodh Patra Pratiyogita at CIRG,
Makhdoom. On 25.09.2013.
24. Pathak, Juhi, Agarwal, Surbhi, Kharche, S.D.,
A.K. Goel, S.K. Jindal, S.K. Agarwal. (2014).
Development potency of in-vitro matured
caprine oocytes after different chemical
activation treatments in modified charles
rosenkrans 2 amino acid medium (MCR2aa).
National Symposium on Frontier
Reproductive Biotechnologies for enhancing
Animal fertility and fecundity global
perspective and XXIX annual convention of
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96
India society for study of animal
reproductive held at MAFSU, Nagpur,
January 8-10, 2014, pp 358.
25. Paul S, Sharma D K, Shivasharanappa N,
Nitika Sharma, Mishra A K, Pawaiya RVS and
Kumar A, 2014. Epidemiology of caprine
cryptosporidiosis in semi-arid region of
north-western India. In: Compendium of
national symposium on Towards food
security through sustainable animal
production and integrated parasite
management held at College of Veterinary
and Animal Sciences, Mannuthy, Thrissur,
Kerala on Feb 5-7, 2014. Abstract P-53, pp.
153.
26. Paul S, Sharma D K, Shivasharanappa N,
Sharma N, Mishra A K, Pawaiya RVS and
Kumar A, 2014. Cryptosporidiosis in
neonatal goats in semi-arid regions of north-
western India. In: Compendium of national
symposium on Towards food security
through sustainable animal production and
integrated parasite management held at
College of Veterinary and Animal Sciences,
Mannuthy, Thrissur, Kerala on Feb 5-7, 2014.
Abstract S 1.32, pp. 56.
27. Paul Souvik, AK Tiwari, DK Sharma, AK
Mishra, Shivasharanappa N, Nitika Sharma,
VK Gupta, Ashok Kumar, Vinay Chaturvedi
and RVS Pawaiya. 2013. Sero-prevalence and
molecular detection of Toxoplasma gondi
isolates from goats in Semiarid region of
India. International symposium on Latest
trends in Immunodiagnosis,
Immunopathology and immunomodulation
and VI Convention of Society for
Immunology and Immunopathology at
Rajasthan University of Veterinary and
Animal sciences from Dec 22-24th, 2013.
Journal of Immunology and
28. Pawaiya RVS, Shivasharanappa N, Sharma
N, Mishra A K, Gururaj K, Paul S, Gupta VK,
Kumar A, Sharma D K, Singh SV, Kumar N,
Chaturvedi V, Tiwari H A, Kumar V and
Dixit AK, 2014. Mortality due to respiratory
diseases in goats: A retrospective study. In:
Compendium of national seminar on Sheep
and goat biodiversity and breeding policies:
Issues and prospective held at Shirwal,
5-2, pp. 506.
29. Pawaiya RVS, Waseem A T, Shivasharanappa
N, Nitika Sharma, A K Mishra, Souvik Paul,
K Gururaj, VK Gupta and Ashok Kumar.
(2013). Parasitic encephalitis due to Oestrus
ovis larva in the brain of a goat. National
symphosium on Advances and Applications
of Diagnostic Pathology for Disease
management in Livestock, Poultry, Fish, Pet,
Laboratory animals and wildlife organized
by Veterinary Pathology Congress-2013 and
Indian Association of Veterinary Pathologist
at College of Veterinary Science & Animal
Husbandry, OUAT, Bhubaneswar, Nov 21-
23rd, 2013.
30. Rai, B., Kumar, R., Ramachandran, N., Dixit,
H. and Rai, R.B. (2014). Village based goat
feeding system in eastern part of U.P.
Opportunities and challenges. in National
held at CVS, Anand (Gujrat), 28-30 January,
2014, pp 153.
31. Raj Priyanka, Ashok Kumar , V K gupta ,
Nitika shrama ( 2014) Biochemical and TLC
based evaluation of plant extract for
antioxidant activity. International
Symposium on The 21st Century Road Map
of Veterinary practice , education and
research in India and developing countries
and XXXII Annual ISVM convention at
Jammu ( 14-16 Feb,2014) Pp 153
32. Rajkumar, V., Verma, A.K. and Das, A.K.
(2013). Development of Healthier Chevon
Nuggets: P/S ratio standardization by the
blend of animal fat and vegetable oil. In:
Souvenir, Seventh International Food
Convention (IFCON-2013) on Nutritional
Security through Sustainable Development,
Research & Education for Healthy Foods
December 18-21, 2013 at CFTRI, Mysore
Karnataka. pp. 121-122.
33. Ramachandran, N., Singh, N.P., Ranjan, R.,
Gangwar, Chetna and Goel, A.K. (2014).
Effect of management systems and seasons
on semen freezability in Jammunapari bucks.
Food Security and Animal welfare held at
Anusandhan Sansthan, Mathura, 19-21
November, 2013.pp 98-99.
34. Ramachandran, N., Tripathi, M.K., Rai, B.,
Singh, S.K., Kumar V. and Jindal S.K. (2014).
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97
Effect of castration on growth, feed intake
and feed efficiency in Barbari kids.
Opportunities and challenges in National
held at CVS, Anand (Gujrat) pp. 28.
35. Ramachandran, N., Tripathi, M.K., Rai, B.,
Singh, S.K., Rajkumar, V. and Jindal, S.K.
(2014). Effect of castration on growth, feed
intake and feed efficiency in Barbari kids. In:
Abstract No. B14. National Seminar on New
Dimensional Approaches for Livestock
Productivity and Profitability Enhancement
under Era of Climate Change & XXI Annual
Convention of ISAPM held at AAU, Anand,
Gujarat, January 28-30, pp 35-36.
36. Ranjan, R., Kharche, S.D., Goel, A.K., Jindal,
S.K., Sikarwar, A.K.S., Pathak, Juhi, Agarwal,
Surbhi, Ranjan, P.R. and Agarwal, S.K. (2014).
Effect of fetal sex on confluences of goat fetal
skin fibroblast cell monolayer. Sheep and
Goat Biodiversity and breeding policies-
Issues and perspective and Annual
conference of Indian Society for sheep & Goat
Production, Krantisinh Nana Patil College
of Veterinary Science, Shirwal (Satara) 21-22
February 2014, pp 262.
37. Ranjan, R., Ramachandran, N., Kharche, S.D.,
Goel, A.K., Gangwar, Chetna, Kumar, S.,
Jindal, S.K. and Agarwal, S.K. (2014). Effect of
egg yolk levels and equilibration periods on
freezability of Jamunapari Goat semen.
International conference on reproductive
health: Issue and strategies under charging
climate scenario held at IVRI, Izatnagar,
Bareilly, 6-8 February 2014, pp 83.
38. Ranjan, R., Singh, Renu, Kumar K., Sarkar,
M., Das, B.C. and Bag, S. (2013). Effect of
cytochalasin B during oocyte maturation for
parthenogentic embryo generation in capra
hircus. National Symposium on Frontier
reproductive biotechnologies for enhancing
animal fertility and fecundity global
perspective and XXIX annual convention of
India Society for Study of Animal
Reproductive held at MAFSU, Nagpur, 8-10
January 2014, pp 347.
39. Ranjan, R., Singh, Renu, Kumar, Kuldeep,
Kharche, S.D., Jindal, S.K., Gangwar, Chetna,
Sarkar M., Das, B.C., Bag, S. and Agarwal S.K.
(2013). Expression profile of H19, Igf2r, Igf2
and Nnat gene during preimplantation
development in Capra hirus. XXII Annual
Conference of Society of Animal
Physilologist of India and National
Symposium on Physiological and Nutri-
genomics interventions to augment food
security and Animal Welfare, held at
Department of Veterinary Physiology U.P. Pt.
Vigyan Vishwavidyalya evan Go-
Anusandhan Sansthan, Mathura, November
19-21, 2013, pp 38.
40. Reddy M, Singh R, Singh K P, Sumana K,
Yadav J, Cherian S, Mishra A K, Patil S S and
Rahman H , 2014. Comparative analysis of
three different tests for diagnosis of animal
rabies India. In: Compendium of
international symposium on Progress in
Veterinary Research: Impact and
Opportunities held at Bangalore on Feb 20-
24, 2014.Abstract, pp. 153
41. Reddy, B.S., L.B.Hugar, A.K.Dixit and
Narendra Singh (2013). Growth and
Variability in Vegetable Production in
Karnataka. Agricultural Economics Research
Review, 26 (Conference Number), 2013 pp
79-88.
42. Sharma Nitika, Ashok Kumar, Ravindra
Kumar A K Mishra Vinay Chaturvedi,
Shivasharanappa N and Singh MK. (2013).
Haematobiochemical indices in peri-
parturient goats. Society of Animal
Physiologist of India, XXII Annual National
Conference and National Symposium on
"Physiological and Nutri-genomic
Interventions to Augment Food Security and
Animal Welfare" at DUVASU, Mathura on
Nov. 19-21, 2013. Page no 63.
43. Sharma, Nitika, Ashok Kumar, Ravindra
Kumar, Shivasharanappa N, A K Mishra,
Vinay Chaturvedi, and RVS Pawaiya. (2013).
Variations of energy biochemical metabolites
in periparturient Barbari goats. Society of
Animal Physiologist of India, XXII Annual
National Conference and National
Symposium on "Physiological and Nutri-
genomic Interventions to Augment Food
Security and Animal Welfare" at DUVASU,
Mathura on Nov. 19-21, 2013. Page no 64.
44. Sharma, Nitika, Ashok Kumar, Ravindra
Kumar, Shivasharanappa N, A K Mishra,
Vinay Chaturvedi, RVS Pawaiya and MK
Singh. 2014. Relationship between serum
glucose, beta-hydroxybutyrate and urea
concentration in pregnant Barbari goats. In:
Compendium of National Seminar on
animal health and production, Society of
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Veterinary Science and Biotechnology,
DUVASU, Mathura, 6-7th March, 2014. p.no
58.
45. Sharma, Nitika, Reena M, Shivasharanappa
N, AK Mishra, Souvik Paul and Vinay
Chaturvedi. 2013. Evaluation of Immune
response of autogenous Staphylococcus
aureus bacterin in mouse model mastitis.
International symposium on Latest trends in
Immunodiagnosis, Immunopathology and
immunomodulation and VI Convention of
Immunopathology at Rajasthan University of
Veterinary and Animal sciences from Dec 22-
24th, 2013. Journal of Immunology and
46. Shivasharanappa, N, Pawaiya RVS, Sharma
N, Mishra A K, Gururaj K, Paul S, Gupta VK,
Kumar A, Sharma D K, Kumar N and Singh
SV, (2014). Pathology of mycotic pneumonia
in sheep. In: Compendium of national
seminar on Sheep and goat biodiversity and
breeding policies: Issues and prospective
held at Shirwal, Maharashtra on Feb 21-22,
2014. Abstract AB-5-1, pp. 506.
47. Shivasharanappa, N, RVS Pawaiya, AK
Mishra, Nitika Sharma, VK Gupta, K
Gururaj, Waseem A T and Ashok Kumar.
(2013). Pathological and microbiological
studies of pulmonary affections in goats and
sheep. National symphosium on Advances
and Applications of Diagnostic Pathology for
Disease management in Livestock, Poultry,
Fish, Pet, Laboratory animals and wildlife
organized by Veterinary Pathology
Congress-2013 and Indian Association of
Veterinary Pathologist at College of
Veterinary Science & Animal Husbandry,
OUAT, Bhubaneswar, Nov 21-23rd, 2013.
48. Shivasharanappa, N, Sharma A, Singh R.,
Kataria M., Dandpat S., Telong A.G., Gupta
V.K and Nitika Sharma. (2013). Effect of T-2
toxin (Trichothecenes) on haematological
and oxidative stress parameters in goats.
Society of Animal Physiologist of India, XXII
Annual National Conference and National
Symposium on "Physiological and Nutri-
Security and Animal Welfare" at DUVASU,
Mathura on Nov. 19-21, 2013. Page no 64.
49. Shivasharanappa, N., V. K. Gupta, G. B.
Manjunatha Reddy, R. V. S. Pawaiya, Ashok
Kumar and S. B. Barbuddhe (2013). Isolation
and characterization of Listeria
monocytogenes from goat brains. 18th
International Symposium on Problems of
Listeriosis (ISOPOL XVIII), ICAR Research
complex, Goa. 9-22 September, 2013.
50. Singh, M. K., Rai, B., Dixit, A.K., Dige, M.S.,
Ramachandran N. and Singh, S.K. (2014).
Management practices of goats in
Bundelkhand region. National seminar on
policies issues and perspective held at
Mahabaleshwar (Satara), 21-22 February,
2014, pp 186.
51. Singh, M.K., Singh, S.K., Bhusan, S.,
Ramachandran, N. and Dige, M.S. (2014).
Morphological, physical and production
characteristics of Bundelkhandi breed of goat
under range based feeding condition.
National Seminar on sheep and goat
biodiversity and breeding policies-issues and
perspective & Annual Convention of
ISSGPU held at Krantisinh Nana Patil
College of Veterinary Science, Shirwal
(Satara), February 21-22 2014, p544.
52. Singh, Narendra, A.K.Dixit., B.S.Reddy and
Surendra B. Kuthe (2013). Rice Production
and its Instability in Gujarat: A
Decomposition Approach. Agricultural
Economics Research Review .. 26 (Conference
Number) 2013 pp 79-88.
53. Singh, S.P., Hussler, S., Dnicke, S., Mielenz,
M. and Sauerwein, H. (2013).
Characterization of serum adiponectin
during lactation in dairy cows supplemented
with conjugated linoleic acids. Joint Annual
Meeting of American Dairy Science
Indiana, USA, 08 12 July 2013. J. Dairy Sci.
Vol. 96 (E-Suppl. 1): 208209.
54. Singh, S.P., Hussler, S., Gross, J.J.,
Bruckmaier, R.M. and Sauerwein, H. (2013).
Adiponectin concentrations in cows milk
during induced negative energy balance.
Indianapolis, Indiana, USA, July 0812 2013.
55. Singh, S.P., Hussler, S., Tesfaye, D., Hlker,
M., Schellander, K. and Sauerwein, H. (2013).
Characterization of follicular fluid
adiponectin and its relationship with blood
adiponectin during estrous cycle in cattle.
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99
Indianapolis, Indiana, USA, July 0812 2013.
56. Singh, S.P., Hussler, S., Wellnitz, O.,
Bruckmaier, R. M. and Sauerwein, H. (2013).
Influence of intramammary
lipopolysaccharide challenge on milk and
plasma adiponectin in dairy cows. J oint
Annual Meeting of American Dairy Science
Indiana, USA, 0812 July 2013. J. Dairy Sci.
Vol. 96 (E-Suppl. 1): 572.
57. Singh, S.P., Heinz, J., Dnicke, S., Hussler, S.
and Sauerwein, H. (2013). Identification of
adiponectin in bovine milk and
characterization of its concentrations during
early lactation. 15th International
Conference on Production Diseases in farm
animals (ICPD), Swedish University of
Agricultural Sciences, Uppsala, Sweden, June
2428 2013, pp 129.
58. Singh, S.P., Mielenz, B., Mielenz, M., Dnicke,
S., Rehage, J., Hussler S. and Sauerwein, H.
(2013). Towards characterizing the usefulness
of serum adiponectin concentrations to
estimate the risk for metabolic diseases in
dairy cows. 16th International Symposium
of the World Association of Veterinary
Laboratory Diagnosticians (WAVLD),
Berlin, Germany, June 0508 2013, pp 339.
59. Singh, S.P., Sauerwein, H., Steyer, M., Ettle,
T., Rodehutscord, M., and Hussler, S. (2013).
Relationships of leptin and adiponectin
serum concentrations with measures of body
condition in Simmental cows. 15th
International Conference on Production
Diseases in farm animals (ICPD), Swedish
University of Agricultural Sciences, Uppsala,
Sweden, June 2428, 2013, pp 139.
60. Singh, S.P., Singh, I., Gangwar, Chetna and
Kumar, P. (2014). Postnatal changes in the
Hematological parameters in the Guinea
fowl. XXII Annual Conference of Society of
Anusandhan Sansthan, Mathura, 19-21,
November 2013, pp 108.
61. Singh, Vikas Kumar, V.K. Gupta,
Shivasharanappa N, Ajay Singh, Elaiyaraja G.
(2013). Study of Differential Expression of
Toll-like receptors (TLR-2, 4 and 9) in natural
caprine brucellosis by using real time RT-
PCR assay. XX Annual convention of Indian
Society for Veterinary I mmunology and
Biotechnology & National Symposium on
"Emerging Challenges & Opportunities in
Veterinary Immunology & Biotechnology for
Improved Animal Health & Productivity"
College of Veterinary & Animal Sciences,
CSKHPKV, Palampur-176062, Himachal
Pradesh. November 11-13th, 2013.
62. Tanuja, Pathak. V., Verma, A.K., Goswami, M.
and Singh V.P. (2013). Development and
quality evaluation of chicken meat momos.
In: Souvenir, Seventh International Food
Karnataka. pp. 118-119.
63. Tripathi, M.K., Gupta Bhawna, Tripathi
Prabhat, Chaudhary U.B. and Kumar
Ravindra. (2013). In-vitro methane
production of protein supplements (Brassica
juncea, Linum usitatissimum, Araches
hypoges, Glycine max, Gossypium
herbaceum, Cyamopsis teteragonoloba and
Sesbania sesban) used in goat feeding. XXII
annual conference of society of animal
physiologists of India and National
Symposium on Physiological and Nutri-
Security and Animal Welfare from November
19-21, 2013 at DUVASU,Mathura, p 25.
64. Tripathi, M.K., Tripathi Prabhat, Gupta
Bhawna Chaudhary U.B. and Kumar
Ravindra. (2013). Methane production
potential of wheat Straw (Triticum aestivum)
based feeding system with whole goat rumen
flora in-vitro. XXII annual conference of
society of animal physiologists of India and
Food Security and Animal Welfare from
November 19-21, 2013 at DUVASU, Mathura,
p 26.
65. Tripathi, M.K., Tripathi Prabhat, Meena
Ramkesh, Gupta Bhavna, Kumar Ravindra
and Chaudhary, U.B. 2014. Nutrient densities
and methane production potential in goats of
tree foliages in semi-arid India. Proc. World
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100
Congress on Agroforestry February 10-14,
2014, New Delhi, India.
66. Tripathi, Prabhat, Tripathi M.K., Dutta T.K.,
Kumar Ravindra and Chaudhary U.B. 2014.
Ber (Zyziphus sp.) based rainfed silvipasture
system for goat production under semiarid
conditions. Proc. World Congress on
Agroforestry February 10-14, 2014, New
Delhi, India.
67. Umaraw, P., Pathak, V., Rajkumar, V., Verma,
A.K., Singh, V.P. and Goswami, M. (2013).
Fatty acid profile of chevon and edible by-
products of Barbari goat kid. In: Souvenir,
Seventh International Food Convention
(IFCON-2013) on Nutritional Security
through Sustainable Development, Research
& Education for Healthy Foods December
18-21, 2013 at CFTRI, Mysore Karnataka. P.
183.
68. Verma, A.K., Rajkumar, V. and Das, A. K.
(2013). Effect of replacing goat fat with
vegetable oils on the quality characteristics
and fatty acid profile of chevon nuggets. In:
Souvenir, Seventh International Food
Karnataka. pp 182.

Human Resource Development
Scientist deputed/Trained in India / Abroad
ICAR International fellowship (Dr
R.Priyadharshini -continuing her Ph.D.
programme in Germany)
ICAR International fellowship.(Dr. S.P.Singh
-completed Ph.D. programme from
University of Bonn, Germany)
International Training on New techniques
for sustainable sheep and goat prodcution.
from 26th Jan-6th Feb, 2014 held at Amman,
Jordan (Dr. N. Ramachandran)
International Training Programme on
Quality Growth Services (ISO 9001:2008) on
February 22, 2014.(S.D.Kharche, Vij ay
Kumar, Ashok Kumar, P.K.Rout)
Management development programme on
leadership at NAARM Hyderabad , 26
th

August -7
th
September 2013 (Ashok Kumar)
Management development programmes on
leadership development 25 Nov to 7 Dec
2013 at National Academy of Agricultural
Research Management, Raj endranagar,
Hyderabad (Andhra Pradesh). (Braj Mohan,
Saket Bhushan)
Review meeting on progressive control of
PPR, organized by FAO (SAARC) at
Kathmandu, Nepal from 19-20 December
2013 ( Naveen Kumar)
Training programme on Advances in
Methodological Paradigm and Tools in
Extension Research Sponsored by ICAR, at
Division of Extension Education, IARI, New
Delhi from 17th Sept. to 7th Oct. 2013 ( Vij ay
Kumar).
Training programme on Market, Trade and
Institutions for Agricultural Development 27
January to 16 February (21 days) at Division
of Agricultural Economics, IARI, New Delhi-
12 sponsored by CAFT, ICAR (Dr. A.K.Dixit)

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101
Training organized
A Post doc fellow Scholar ( Dr Erick Virgile
Azando From Republic of Benin ) under C V
Raman International Fellowship was guided by
Dr Ashok Kumar Principal Scientist on Effect
of local anthelmintic plants on gastrointestinal
nematodiasis in small ruminants ( 15 Sep to 14
Dec 2013)

Official language programmes
Hindi pakhwada was organized from
13.9.2013 to 28.9.2013 and several
programme like Hindi Hastakshtra
Pratiyogta, Hindi Anuprayog Pratiyogta,
Hindi Sodh Patra Partiyogta, Sulekh
Pratiyogta etc. were organized.
Quarterly meeting of Raj ya Bhasha
Karyanwan Samiti were organized on 11
th

June, 2013; 4
th
Sept. 2013 and 4
th
March, 2014.
The Hindi Karyashalas were organized on
29.5. 2013, 26.9.2013 and 26.12.2013 at CIRG
and several programmes and guest lectures
were organized.
Gene sequences published
1. Gupta,V.K., Gururaj,K., Shivasharanappa,N.,
Singh,V.K., Trivedi,R.N., Singh,A., Rout,P.K. and
Kumar,A (2014). Capra hircus breed Jamunapari
Toll like receptor-2 protein (TLR-2) gene,
complete cds. Gene Bank Accession: KJ183648
Trivedi,R.N., Singh,V.K., Singh,A., Rout,P.K. and
Kumar,A (2014). Capra hircus breed Jamunapari
Kumar,A (2014). Capra hircus breed Barbari Toll
like receptor-1 protein (TLR-1) gene, partial cds.
Gene Bank Accession: KJ210570
4. Gupta,V.K., Shivasharanappa,N., Gururaj,K.,
Kumar,A (2014). Capra hircus breed Sirohi Toll
like receptor-3 protein (TLR-3) gene, complete
cds. Gene Bank Accession: KJ210565
5. Gururaj, K., Gupta,V.K., Shivasharanappa,N.,
kumar,A. (2014). Capra hircus breed Sirohi Toll
6. Gururaj, K., Shivasharanappa N., Gupta,V.K.,
kumar,A. (2014). Capra hircus breed Jakhrana
7. Gururaj, K., Shivasharanappa, N., Gupta,V.K.,
Trivedi,R.N., Singh,V.K., Singh, A., Rout, P.K. and
8. Gururaj,K., Gupta,V.K., Shivasharanappa,N.,
kumar,A. (2014). Capra hircus breed Sirohi Toll
9. Gururaj,K., Gupta,V.K., Shivasharanappa,N.,
kumar,A. (2014). Capra hircus breed Jamunapari
10. Kumar, N., Chaubay,K.K., Chaudhary,K.,
Sharma,S., Singh,S.V. and Sharma,D.K. Peste-des-
petits-ruminants virus strain
PPRV/Nanakpur/2012 nucleoprotein (N) gene,
partial cds. GenBank Accession: KC200262.1
11. Kumar, N., Kachhawa,S., Kashyap,S.K.,
Maherchandani,S., Singh,S.V., Chaubey,K.K.,
Gupta,S. and Rawat,K.D.Peste-des-petits-
ruminants virus strain Nagaur1 fusion protein
mRNA. GenBank Accession: KJ081283.1
12. Kumar, N., Wadhwa,A., Chaubey,K.K.,
Singh,S.V., Gupta,S., Sharma,S.,Sharma,D.K.,
Singh, M.K. and Mishra,A.K., Orf virus isolate
CIRG major envelope protein gene, partial cds.
GenBank Accession: KC992325
13. Mishra, A K, Gururaj K, Gupta G, Gupta V K,
Kumar N, Shivasharanappa N, Sharma N and
Paul S (2014). Staphylococcus aureus strain
CIRG-SM1 thermostable nuclease gene, partial
cds. GenBank Accession: KF765737.
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102
Paul S (2014). Escherichia coli strain CIRG-ECD1
universal stress protein A (uspA) gene, complete
cds. GenBank Accession: KF765738
Paul S (2014). Escherichia coli strain CIRG-ECM1
Paul S (2014). Escherichia coli strain CIRG-ECP1
Paul S (2014). Escherichia coli strain CIRG-ECS1
Shiga toxin gene, partial cds.GenBank Accession:
KF765741.
18. Shivasharanappa, N, Gupta, V K, Gururaj K,
Manjunathreddy GB, Kumar A and Rout P K
(2013). Capra hircus breed Barbari Toll-like
receptor 2 (TLR2) gene complete cds. Gene Bank
Accession: KF765736.
19. Shivasharanappa, N, Gupta, V K, Gururaj K,
Toll-like receptor 1 (TLR1) gene partial cds. Gene
Bank Accession: KJ210569
20. Shivasharanappa, N, Gururaj K, Gupta, V K,
kumar,A. (2014). Capra hircus breed Barbari Toll-
like receptor 3 (TLR3) gene complete cds. Gene
Bank Accession: KJ210566

Conference/ Seminar/ Symposium/Workshop attended
Annual conference of society of animal physiologists
of India and National Symposium on
Physiological and Nutri-genomic
Interventions to Augment Food Security and
Animal Welfare from November 19-21, 2013
at DUVASU, Mathura, India. ( M.K.Tripathi,
Nitika Sharma, Vinay Chaturvedi, Chetna
Gangwar and S.D.Kharche)
Annual Convention of Indian Society for Veterinary
Immunology & Biotechnology & National
Symposium on Emerging Challenges &
Opportunities in Veterinary Immunology &
Biotechnology for Improved Animal Health
& Productivity, held at Veterinary College
Palampur, HP, India from November 11 to
13, 2013 ( Naveen Kumar)
Annual Convention of the Indian Society for Study of
Animal Reproduction (ISSAR) and National
Symposium on Frontier Reproductive
Biotechnologies for Enhancing Animal
Fertility and Fecundity: Global Perspective,
8-10 January 2014, Nagpur Veterinary
College, MAFSU, Nagpur (M S).( A.K.Goel,
S.D.Kharche, Ravi Ranjan)
ANGR Country Report meeting held at Karnal on
from 20-12-2013 to 22-12-2013 (S.K.Singh)
Annual KVK meet at GKVK, Bangalore, from 22-25,
10-2013 (S.K.Singh)
Annual meeting of society of Veterinary science and
Biotechnology and national seminar on
Biotechnologies approaches to challenges in
animal health and production organized by
department of Biochemistry held at U.P. Pt.
Vigyan Vishwavidyalya evan Go-
Anusandhan Sansthan, Mathura, 6-7 March,
2014.( S.D.Kharche, Chetna Gangwar,
A.K.Mishra, Nitika Sharma, V.K.Gupta,
Ashok Kumar, K.Gururaj, N.
Shivasharanappa, Dige Mahesh Shivanand)
Annual Review Meeting of Net Work Project on
Sheep Improvement and Mega Sheep Seed
Project held at CSWRI, Guest House, Jaipur
from, 16-17 November, 2013. (Gopal Dass)
Annual Review Meet of All Indian Coordinated
Research Project on Goat Improvement held
at MPKV, Rahuri held on 6-7 sept 2013 ( Dige
Mahesh Shivanand).
Annual Review Workshop of the NFBSFARA projects
on July 22-23, 2013 at NASC Complex, Pusa,
New Delhi 110012. ( S.D.Kharche)
Awareness Programme on Quality Management
system (ISO-9001:2008, Fourth Edition,
Quality Growth) from New Delhi at CIRG,
Makhdoom on 27.07.2013.( All scientists of
Institute)
Brain Storming on Strategy related to conservation
and productivity enhancement of farm
animal genetic resourcesjointly organized
by ICAR and TAAS at NASC Complex, DPS
Marg, New Delhi on 10 January, 2014. (Gopal
Dass)
CAC meeting of NAIP-3 project Goat husbandry
based integrated approach for livelihood
security in disadvantaged district of
Bundelkhand region held on 01.03.14 at
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103
CIRG, Makhdoom. (All Heads, component
workers and scientists of GGB Division)
Directors Conference held at Pune and Baramati, from
18-01-2014 to 21-01-2014(S.K.Singh)
Farm Innovation Day held on 27.04.04 at CIRG,
Makhdoom. (All scientists of Institute)
Interactive meeting on Prospects in improving
production, Marketing and value addition of
carpet wool organized by Arid Region
Campus of CSWRI Avikanagar at Bikaner, 31
December, 2013. (S.D.Kharche)
International conference on Reproductive Health:
Issue and Strategies under Changing Climate
Scenario, organized by Division of
Physiology and Climatology at IVRI,
Izatnagar Bareilly, February 6-8, 2014.(
S.D.Kharche, Ravi Ranjan, Chetna Gangwar)
International conference on Emerging and
Transboundary diseases of Global
importance held at Madras veterinary
college on 15-16th July 2013, jointly
organized by TANUVAS (India), University
of Nottingham (UK) and Virginia Maryland
regional college of veterinary medicine (USA)
( K.Gururaj)
International Conference on Production Diseases in
Farm Animals (ICPD), Uppsala, Sweden,
June 24-28, 2013. (S.P.Singh)
International Seminar on Vishva ki pragati men
vigyan evam prodyogiki ka yogdan held at
Metkalf House, DRDO, Ministry of Defence,
New Delhi from 05-07 December, 2013.
(Gopal Dass)
International Symposium and Annual Convention of
Immunopathology on Latest trend in
immunodiagnosis, immunopathology and
immunomodulation organised by
RAJUVAS, Bikaner during 22nd 24th Dec,
2013. ( A.K.Mishra, Souvik Paul and Nitika
Sharma)
International Symposium of the World Association of
Veterinary Laboratory Diagnosticians
(WAVLD), Berlin, June 5-8, 2013(S.P.Singh)
International Symposium on The 21st Century Road
Map of Veterinary practice , education and
research in India and developing countries
and XXXII Annual ISVM convention at
Jammu ( 14-16 Feb,2014) (Ashok Kumar)
Joint American Dairy Science Association (ADSA) -
American Society of Animal Science (ASAS)
Annual Meeting, Indianapolis, Indiana, USA,
July 8-12, 2013. (S.P.Singh)
Meeting of AICRP on Goats and KVKs of UP to
develop collaborative program for genetic
improvement of goats which was held on
12.02.14 at CIRG, Makhdoom ( All heads and
scientists of GGB Division).
Meeting with BAIF officials for discussion on Mutual
Areas of Interest for working together in the
field of goat production held on 21.05.13 at
CIRG, Makhdoom (All scientists of Institute)
National Seminar on Biotechnological approaches to
challenges in Animal Health and
Production at DUVASU Mathura UP ( 6-7
March 2014) (Ashok Kumar, A.K.Mishra and
Nitika Sharma )
National seminar on New technology of agricultural
and allied sciences: Achievements &
challenges (in Hindi language) 11-13
December 2013 at Central Institute of
Fisheries Education, (I.C.A.R.) Versova,
Mumbai. (Saket Bhushan)
National Symposium Harmonizing phenomics and
genomics for sustainable management of
livestock for upliftment of rural masses
organized by SOCDAB, NBAGR, Karnal 6-7
February, 2014 at NBAGR, Karnal. (Saket
Bhushan, Dige Mahesh Shivanand )
Quarternly Hindi Workshop held on 26.12.2013 at
CIRG , Makhdoom ( Attended by all staff of
CIRG)
Seminar on Challenges and strategies for
conservation of small ruminants in India
from 9-10 October, 2013 at DUVASU,
Mathura, UP.( Gopal Dass)
Society of Nutrition Physiology Conference,
Gttingen, Germany, March 19-21, 2013.
(S.P.Singh)
Workshop/seminar on Conservation of animals ICAR,
New Delhi from 09.01.2014 to
11.01.2014(S.K.Singh)
Workshop on Physiology and Biotechnology of Milk
Removal at Veterinary Physiology, Vetsuisse
Faculty, University of Bern, Switzerland,
April 25-26, 2013. (S.P.Singh)
Workshop on Professional Skills by Zentralstelle fr
Schlsselkompetenzen, University of Bonn,
August 12-13, 2013. (S.P.Singh)
World Congress on Agroforestry, February 10-14,
2014, New Delhi, India. (M.K. Tripathi,
Prabhat Tripathi and Ravindra Kumar )
CIRG

Important Meetings
Composition of the Research Advisory Committee
Dr.V.Prabhakar Rao,Vice Chancellor, Sri Venkateswara Veterinary
University, Tirupati
Chairman
Dr.N.Krishnan, Ex Associate Dean, Hyderabad Member
Dr.S.K.Dwivedi, Ex. Director, NRC on Equines, Hisar Member
Dr.R.J.Sharma,Ex. Deam,Mathura Member
Dr.K.Kumanan,Prof. and Head, Madras Veterinary College, Chennai Member
Dr. S.N.Maurya, Former Vice Chancellor, DUVASU, Mathura Member
Shri Ashok R Kale, 21, Kisan Kranti, Market Yard, Ahmednagar 414001,
Maharashtra
Member
Shri K Venkatesh, Vijay Farms, Villupuram, Tamil Nadu Member
Dr. S.K.Agarwal, Director, CIRG, Makhdoom Member
ADG (AN&P),ICAR Member
Dr.P.K.Rout, Principal Scientist, CIRG and Incharge, PME Member Secretary

Composition of the Institute Management Committee
Dr. S.K.Agarwal, Director, CIRG, Makhdoom Chairman
Director, Animal Husbandry, Uttar Pradesh, Lucknow Member
Director, Animal Husbandry, Uttrakhand Member
Vice Chancellor, Pt. Deen Dayal Upadyay Pashu Chikitsa Vigyan
Vishwavidyalaya evam go anunsandhan Sansthan, Mathura
Member
Shri, S.K.Pathak, DD(F-III), ICAR, Krishi Bhavan, New Delhi Member
Dr. Sanjeev Kumar, Senior Scientist, NBAGR, Karnal Member
Dr. Taru Sharma, PS & Head,
Animal Physiology, IVRI, Izatnagar
Member
Dr. Dhirendra Singh, PS, Animal Health, CSWRI, Avikanagar Member
Dr. S.K.Singh, PS, AG&B, CIRG, Member
Shri Ashok R Kale, 21, Kisan Kranti, Market Yard, Ahmednagar 414001,
Maharashtra
Member
Shri K Venkatesh, Villupuram, Tamil Nadu Member
ADG(AN&P),ICAR Member
Administrative Officer,CIRG, Makhdoom Member Secretary

CIRG

Institute Research Committee (IRC)
The half yearly IRC of the Institute was held on
30-31
st
Oct, 2013 and progress of all Institute and
external funded proj ects was reviewed. The
meeting was chaired by Dr. S.K.Agarwal,
Director CIRG and attended by all the scientists
of the Institute.
Institute Management Committee (IMC)
The Institute Management Committee meeting
was held on 12
th
June, 2013. The meeting was
attended by Dr. A.C.Varshney, VC, DUVASU,
Mathura and member IMC, Dr. Dhirendra Singh,
Principal Scientist, CSWRI and member RAC, Dr.
Sanj eeva Kumar, NBAGR, Karnal and Dr.
G.Taru Sharma, Head and Director, CAS,
Physiology and Climatology Division, IVRI and
Mr. R.N.Mallik, A.O. and member secretary,
IMC. The committee discussed various issues
related to Institute and appreciated the
achivements of the Institute scientist in the area
of goat production and health management.

Director, CIRG Dr. S.K.Agarwal chaired the
meeting and thanked the members for their
contribution.
Research Advisory Committee (RAC)
The meeting of Research Advisory Committee
(RAC) of CIRG was held on 29
th
May, 2013 under
the chairmanship of Dr V.Prabhakar Rao,
Chairman RAC, Dr.S.K. Dwivedi and
Dr.K.Kumanen, Dr. N.Krishnan, Dr.
S.N.Maurya, Dr. B.S.Prakash, ADG (AN&P) and
Dr S.K.Agarwal, Director, CIRG were present.
Dr. Prabhakar Rao emphasized on dissemination
of technologies developed by the Institute. He
further empahsised that considering the
importance of women in goat rearing, more
training should be taken up by the Institute for
women goat farmers. Dr. Dwivedi and Dr.
Kumanan also extended suggestions to
strengthen the goat research programmes at the
Institute.

CIRG

106
Research Projects
List of Approved Institute Projects
S.No. Project Title P.I.
Improvement of Jakhrana breed of goats for milk and meat
production under farm and field conditions
Dr. Saket Bhusan
Extension Approaches for Dissemination of Goat Production
Technologies and Impact Assessment
Dr. Braj Mohan
Economic Losses due to Important Diseases in Goat
Production
Dr Anupam Krishna
Dixit
A study on impact assessment of various training programmes Dr. Khushyal Singh
Patho-Epidemiological Studies on Emerging and Existing
Diseases of Goats
Dr. R.V.S. Pawaiya
Effect of Nutritional Deficiency Diseases on Gene Expression
Profiles in Goats
Dr. R.V.S. Pawaiya
Genetic Marker study in Indian Goats for GI nematode
Resistance with special reference to Haemonchus infection.
Dr. DK Sharma
Toll like receptors (TLRs) expression and characterization in
different breeds of goats and their role in disease resistance
with special reference to brucellosis
Dr. VK Gupta
Development of herbal anthelminitic and acaricidal
formulation for goats
Dr. Ashok Kumar

Study on the molecular mechanism of resistance and
susceptibility to PPR virus in goats
Dr. Naveen Kumar
Metabolic profiling for diagnosis and control of metabolic
diseases of goats
Dr Nitika Sharma

Isolation, identification and characterization of maj or
infectious agents associated with neonatal diarrhoea in kids
Dr. A.K. Mishra
Improvement of post-thaw quality and fertility of frozen
semen of different breeds of goats using various additives.
Dr Satish Kumar
Hormone profile during different reproductive stages in goats Dr AK Goel

Comparative Study on Different Structures of Goats Shelters
under Farm Conditions
Dr. N Ramachandran
Traceability, food safety standars and food chain evaluation
(HACCP) pertaining to goat meat and value added products
Dr. V. Raj kumar
Development of complete feed for environmentally and
economically sustainable goat production
Dr. Ravindra Kumar
Value Chain for the Development of Goat Products with
Healthy Traits
Dr. A.K. Verma
Development of feed resources on poor land for goats Dr. P. Tripathi

Out Funded Projects
S.No. Project Title (AICRP Projects) P.I.
Improvement and Sire evaluation of Jamunapari goats for
milk & meat production AICRP Jamunapari Unit
Dr. PK Rout
Network Proj ect on Sheep Improvement Muzaffarnagri Unit Dr.Gopal Dass
AICRP - Improvement of feed resources and nutrient
utilization in raising animal production
Dr. U.B.Chaudhary
CIRG

107
Estimation of methane emission under different feeding
systems and development of mitigation strategies
Dr. M.K. Trpathi
Project Title (NAIP Projects)
Goat Husbandry based integrated approach for livelihood
security in disadvantaged districts of Bundelkhand region
(NAIP comp III)
Dr. M.K. Singh
Bioprospecting of genes and allele mining for abiotic stress
tolerance (NAIP Comp IV)
Dr. P.K. Rout
Achieving Improved Livelihood Security through Resource
Conservation and Diversified Farming Systems Approach in
Mewat (NAIP Component III)
Dr. D.K. Sharma
Developmental potentio parthenogenetic goat embryos
(NAIP).
Dr. S.D. Kharche
Holistic Approach for improving Livelihood Security through
livestock based farming system in Barabanki&RaeBareilly
Districts of U.P. (NAIP Comp III)
Dr. B. Rai
Project Title (External Funded Projects)
Development and Characterization of an Indigenous Vaccine
and Diagnosis for Johnes disease (CSIR and Biovet) NIMTLI
Dr. S.V. Singh
DST proj ect on Development of diagnostic assay,
Molecular characterization and epidemiology of
cryptosporidiosis in goats
Dr. S. Paul
Assessing resilience of small ruminant production under
changing climatic conditions in semi-arid zone (NICRA ).
Dr. U.B. Chaudhary
Development of Parthenogenetic Goat from Embryonic Stem
Cells (NFBSFARA ).
Dr S.D. Kharche
National Referral Laboratory for Testing of Animal Products
(MOFPI ).
Dr. V. Raj kumar
Outreach Programme on Zoonotic Diseases Dr S.V. Singh
VTCC Veterinary Type Culture-Microbes in collaboration
with NRCE, Hissar
Dr. V.K. Gupta
VTCC Veterinary Type Culture-Rumen Microbes in
collaboration with NAINP, Bangalore.
Dr. U.B. Chaudhary

Consultancy, Patents and commercialization of Technologies
Patent application filed:
Patent No 2913DEL/2014 Novel herbal
antimicrobial gel for animals S K Garg ,
Prashant Yadav, Ashok Kumar ( 2014)
Patent No. 3516/DEL/2013 Economic
concentrate pellet feed with Brassica oil cake
for ruminant feeding, chemical composition,
production protocol, storage and uses
thereof Inventors M.K. Tripathi, Prabhat
Tripathi, U.B. Chaudhary, D.L. Gupta and
Ravindra Kumar.
Patent No. 3517/DEL/2013 Oil extracted meal
(cake) less concentrate feed for ruminants:
chemical constituents, production
methodology, storage and uses Inventors
M.K. Tripathi, Prabhat Tripathi, U. B.
Chaudhary, Ravindra Kumar and D.L.
Gupta.
Patent No. Process to Develop Functional
Chevon Nuggets with Healthier Fatty Acid
Profile Inventors A.K. Verma and V.
Rajkumar

CIRG

108
Distinguished Visitors
Dr. G.K.Singh, Dean, COVS, GBPUAT,
Pantnagar. 20.04. 2013.
Dr. Harpal Singh, Ex-V.C., Dean,
GBPUAT, Pantnagar, 19.05.2013.
Dr. A.K.Mishra, V.C., MAFSU, Nagpur,
27.04.2013.
Dr. D.V.Ragnekar, Ex Programme
Coordinator, BAIF, Urulikanchan,
21.5.2013
Dr. D.K. Sharma, Director, CSSRI, Karnal,
20.05.2013.
Dr. B.S. Prakash, ADG (A N & P), Krishi
Bhawan, New Delhi. 28.05.2013.
Dr. V. Prabhakar Rao, V.C., SV Veterinary
University, Tirupati. 28.05.2013.
Dr. K. Kumanan, Director Research,
TANUVAS, Chennai. 28.05.2013
Dr. S.K.Dwivedi, Ex- Director, NRCE,
Member RAC. 29.05.2013.
Dr. S.N. Maurya, Member RAC, Ex-V.C.,
DUVASU, Mathura. 29.05.2013.
Prof. A.C.Varshney, V.C., DUVASU,
Mathura, 12.06.2013.
Dr. V.K. Bhatia, Ex-Director, IASRI, New
Delhi, 13.07.2013.
Dr. Mahendra Johari, Vice- President,
Heifer International, USA, 02.08.2013.
Dr. Gaya Prasad, ADG, Animal Health,
ICAR, New Delhi, 24.08.2013.

Dr. Maureen Valentine, Cornell University,
USA, 23.09.2013.
Prof. P.K. Uppal, Ex-Director, NRCE, Advisor
Punjab Government (A.H.), 09.09.2013.
Dr. H.S.Sandhu, Director, Animal Husbandry,
Punjab. 09.10.2013.
Dr. Anatoly Zherdev, Inst. of Bio-Chemistry,
Moscow, Russia. 23.10.2013.
Dr. V. Brahamandan, Director, Animal
Husbandry, Kerala. 13.11.2013.
Dr. K.M.L.Pathak, DDG(AS), ICAR, New
Delhi 19.11.2013

Dr. B.N. Bhatacharyya, Dir. Res. (Vety.),
Assam Agr. University, Khanapara.
19.11.2013.
Mr. Alok Jain, I.A.S., V.C., GBPUAT,
Pantnagar, 29.11.2013.
Dr.S.D.Singh, ADG (Inland Fisheries),
ICAR, 17.2.2014.
Dr. Dilip Kumar, Ex-Director, CIFE,
Mumbai. 18.02.2014.
Dr. A.P. Srivastava, National Coordinator,
NAIP, New Delhi, 01.03.2014.
Dr. Arun Verma, Ex-ADG, ICAR and
CAC Chairman NAIP. 01.03.2014
Dr. D.S.Singh, Ex-Professor, NDUA&T,
Faizabad, 1.3.2014
Dr. Nagendra Sharma, Ex Director, CIRG,
NDRI, 5.3.2014.
Dr. K.K.Katoch, V.C., CSK HP Agr.
University, Palampur. 06.03.2014.

Dr. V.K.Singh, Ex-Director, CSWRI,
Avikanagar. 24.03.2014.
Dr. B.S. Dwivedi, Head, IARI, New Delhi.
24.03.2014.

CIRG

109
Personnel
Administration
Dr.S.K. Agarwal Director
Dr.P.K.Rout Scientific Secretary
Dr.A.K.Goel VigilanceOfficer
Mr.R.K.Sharma Senior Administrative
Officer
Mr.P.K.Singh Finance and Accounts
Officer
Mr S.S.Gautam Asstt.Admn.Officer
Mr. A.K.Sharma Asstt.Admn. Officer
Mr. C.S.Sagar Asstt.Admn. Officer
Mr.S.R.Achary Private Secretary

Genetics and Breeding Division
Dr. S.K.Singh Principal Scientist and
Head
Dr. Saket Bhushan Principal Scientist
Dr. P.K.Rout Principal Scientist
Dr. Gopal Dass Principal Scientist
Dr. M.K.Singh Sr.Scientist
Mr. Badan Singh Technical OfficerT-5
Mr. A.S.Praj apati Technical OfficerT-5
Mr. Vinod Kumar Technical OfficerT-5
Mr. Gulzari Lal Technical OfficerT-5
Mr. Raj endra
Kumar
Technical OfficerT-5

Physiology,Reproduction and Shelter
Management Division
Dr. S.K.Jindal Principal Scientist and
Head
Dr. Satish Kumar Principal Scientist
Dr. A.K.Goel Principal Scientist
Dr. B.Rai Principal Scientist
Dr. S.D.Kharche Principal Scientist
Dr.
N.Ramachandran
Scientist
Dr. S.P.Singh Scientist
Dr. RaviRanj an Scientist
Dr.
PriyadharsiniRaju
Scientist (on study
leave)
Mr. Krishan Kumar Technical OfficerT-5(
upto 08.12.2013)
Mr. H.K.Himkar

Technical OfficerT-5
Mr. Hari Om Technical OfficerT-5
Mr. Dinesh Bhat Technical OfficerT-5
Nutrition,Feed Resources and Products
Technology Division

Dr. U.B.Chaudhary Pr.Scientist and Head
Dr. M.K.Tripathi Principal Scientist
Dr. R.B.Sharma Principal Scientist
Dr. Prabhat Tripathi Senior Scientist
Dr. Ravindra
Kumar,
Senior Scientist
Dr. V.Raj kumar Sr. Scientist
Dr. A.K.Verma Scientist
Mr. Suresh Tewari Asstt. Chief Technical
OfficerT-7(7-8)
Mr. Dori Lal Gupta Sr. Technical Officer
T-6
Mr. Raj Kumar
Singh
Sr. Technical Officer
T-6
Mr. Suraj Pal Technical OfficerT-5
Mr. Lal Singh Technical OfficerT-5

Goat Health Division
Dr. S.V.Singh Principal Scientist and
Head
Dr. D.K.Sharma Principal Scientist
Dr. Ashok Kumar

Principal Scientist
Dr. V.K.Gupta Principal Scientist
Dr. R.V.S.Pavaiyya Principal Scientist
Dr. Naveen Kumar Senior Scientist
Dr. K.Gururaj Scientist
Dr. Nikita Sharma Scientist
Dr. Shivsharnappa Scientist
Dr. A.K.Mishra Scientist
Dr. Souvik Pal Scientist
Dr. H.A.Tiwari Chief Technical Officer
(T-9)
Dr. Vinay
Chaturvedi
(T-6)
Sr. Vij ay Kishore Technical OfficerT-5
( On study leave)
Sh. Chet Ram Technical OfficerT-5
Sh. V.K.Gautam Technical OfficerT-5
Sh. T.K.Gautam Technical OfficerT-5

Extension Education and Socio-
Economics Section
Dr. Braj Mohan Pr.Scientist and I/c
Dr. A.K.Dixit Senior Scientist
CIRG

110
Dr. Khushyal Singh Scientist (Sr. Scale)
Dr.Vij ay Kumar Scientist
Mr. S.C.L.Gautam Technical OfficerT-5
Mr. U.C.Yadav Technical OfficerT-5

AICRP on Goat
Dr. S.K. Singh Principal Scientist and
I/c
Dr. Shivanand
Mahesh Dige,
Scientist

Network Project on Sheep
Dr. Gopal Dass Principal Scientist

Priortization Monitoring and
Evaluation Section
Dr. P.K.Rout Pr. Scientist and I/c
Dr. Ashok Kumar Principal Scientist
Dr. Souvik Paul Scientist
Dr. Balraj Singh Sr. Technical Officer
T-6

IPR Cell
Dr V.K.Gupta Principal Scientist and
I/c

RTI Cell
Dr V.K.Gupta Principal Scientist and
Transparency Officer
Dr. H.A.Tewari Chief Technical Officer
(T-9) and PIO
Dr. Vij ay Kumar Scientist and APIO

Agriculture Knowledge Management
Unit (AKMU)
Dr. R.V.S.Pavaiyya Principal Scientist and
I/c
Sh. M.P.Agarwal Technical Officer T-5
Sh. Satish Chandra Technical Officer T-5

Maintenance
Dr.U.B.Chaudhary Principal Scientist and
I/c
Sh. Jagdish Singh Technical OfficerT-5
Sh. Ishwari Saran Technical Officer T-5
Sh. Inder Pal Technical OfficerT-5

Security Section
Mr. P.K.Sharma Security Officer
Medical Section
Dr. Ashok Kumar Principal Scientist and
I/c
Mr. Mohan Lal Technical Officer T-5

Library
Dr. A.K.Goel Pr.Scientist and I/c
Dr. Pratap Singh

Chief Technical
Officer, T-9

Agriculture Farm
Dr. Prabhat Tripathi Sr.Scientist and I/c
Sh. Ram Kishan Technical OfficerT-5

Horticulture Section
Dr. B.Rai Pr.Scientist and I/c
Sh. Suraj Technical OfficerT-5
Sh. Hukam Singh Technical OfficerT-5

Transfer
Dr. A.K. Das Scientist transferred
to, IVRI Regional
Station, Kolkata
Mr. R.N.Mallik, AO Transferred to IVRI
Izatnagar
Sh. Kailash Chand,
JAO
Transferred on
promotion to CARI,
Izatnagar

Joining
Shri Raj esh Kumar
Sharma, SAO
w.e.f. 05.07.2013
Shri. P.K.Singh,
FAO
w.e.f. 01.04.2013
Sh. Bacchu Singh,
LDC
w.e.f. 01.10.2013
Dr. Shivanand
Mahesh Dige,
Scientist
w.e.f. 12.04.2013
Dr. Chetna
Gangwar, Scientist
w.e.f. 23.05.2013
Dr. S.P. Singh,
Scientist
w.e.f. 14.02.2014 after
completion of study
leave
CIRG

111
Dr. K. Gururaj ,
Scientist
w.e.f. 01.10.2013 after
completion of study
leave
Dr. Vij ay Kumar w.e.f. 29.05.2013 after
completion of study
leave

Retirement/ Death
Sh. Bhagwan Singh,
Technical Officer
Retired on 31.1.2014
Sh. Jagdish Singh,T-
5
Technical Officer
Sh. Soren Singh SSG Retired on 31.3.2014
Sh. M.P.Agarwal, T-
5, Technical Officer.
Sh. Dinesh Prasad,
T-6
Sh. Krishna Kumar,
T-6 Technical
Officer
Died on 08.12.2013

Career Advancement/Promotion
Shri Lal Singh Promoted to the post
of Technical Officer
(T-5)
Shri. S.C.L.Gautam Promoted to the post
(T-5)
Shri.T.K.Gautam Promoted to the post
(T-5)
Shri. V.K.Sharma Assured Promoted to
the post of
Sr.Technical Assistant
T-4/T-3
Shri.Shiv Charan T-
II=3
Assured Promoted to
the post of
T-4/T-3
Shri.Govind Prasad Assured Promoted to
the post of
T-4/T-3
Sh.Yatendra Kumar Assessed promoted to
the post of
Sr.Technician(T-2)
Sh.RamSwarop Assessed promoted to
the post of
Sr.Technician(T-2)
Sh. R.S.Sarswat Assessed promoted to
the post of
Sr.Technician(T-2)
Sh.Krishna Kumar Assessed promoted to
the post of
Sr.Technician(T-2)

Deputation abroad
Dr. R.Priyadarshini Scientist on study
leave for Ph.D. in
Germany under ICAR
International
Fellowship

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CIRG

Annual Report 2013-14

) via a single electron reduction.

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