Determination of Chlorine Dioxide by DPD Method
Determination of Chlorine Dioxide by DPD Method
BY DPD METHOD
Method Reference:
Standard Methods 19 th Editon (4500 CLO2-D)
Sam!e Conta"ner:
A clean plastic or lass container (250 ! 1000 "l#) a$oid e%cessi$e liht and aitation
On# S"te S$r%e& Re'$"rement(
1& Each '(ll) appro$ed anal)st "(st *e a*le to de"onstrate the constr(ction o' the
D+D cali*ration c(r$e&
2& All reaents, standards and sol(tions (sed 'or cali*ration and anal)sis -ill *e
chec.ed 'or proper la*elin, dates and records&
/& proper proced(ral techni0(e and interpretation o' data -ill *e o*ser$ed&
Interference(
1& 1(r*idit) and color "a) inter'ere -ith the deter"ination o' the a*sor*ance
contri*(tion 'ro" D+D&
2& O%idi2ed "ananese is the "ost sini'icant so(rce o' inter'erence( this t)picall)
is not a pro*le" in 'inished drin.in -ater)& 1o red(ce inter'erence, i' e%cess
"ananese is present, place 5 "l *(''er sol(tion and 0&5 "l sodi(" arsenite
sol(tion in 'las.& Add 100 "l sa"ple and reaents, read a*sor*ance and s(*tract
'ro" readins o*tained in "ethod& A 0&253 sol(tion o' thioaceta"ide can *e
s(*stit(ted 'ro the sodi(" arsenite&
/& 4nter'erence o' copper le$els (p to appro%i"atel) 10 "5L is o$erco"e *) the
ED1A in the D+D sol(tion& 1he ED1A also helps the sta*ilit) o' the D+D
sol(tion&
E'$"ment
1& Standard La*arator) 6lass-are&
2& Class A $ol("etric pipets or an accepta*le "icroliter pipettor 'or standardi2ations
/& A spectrophoto"eter capa*le o' readin 515 n" -ith a cell liht path -idth o'
atleast 1 c"&
4& 6raph paper or a co"p(ter -ith so't-are capa*le o' per'or"in linear
reressions&
Rea)ent(
1. PHOSPHATE BUFFER SOLUTION:
Dissol$e 24 ra"s o' anh)dro(s 7a28+O4 and 49 ra"s o' anh)dro(s :82+O4
in appro%i"atel) ;00 "l o' La*arator) p(re -ater&
Dissol$e 0&< ra"s o' disodi(" ED1A in 100 "l La*arator) p(re -ater
Co"*ine these t-o sol(tions
Dil(te to 1 litre -ith la*orator) p(re -ater
Add 0&0020 o' 8Cl2 as a preser$ati$e&
Store in a"*er lass&
2. DPD INDICATOR
Dissol$e 1&0 o' D+D o%alate, or 1&5 o' D+D s(l'ate pentah)drate, or 1&1 o'
anh)dro(s D+D s(l'ate in Chlorine 'ree la*orator) p(re -ater containin < "l
1=/ 82SO4 ( 9"l -ater = 2 "l 82SO4)
Dissol$e 0&2 o' disodi(" ED1A in the a*o$e sol(tion
Dil(te to 1 Litre and store in a"*er lass&
/& +O1ASS4>M +E?MA76A7A1E SOL>14O7S@
(See D+D Chlorine Method)
4& +O1ASS4>M 4OD4DE, :4 cr)stals
5& 6LAC47E SOL>14O7@
Dissol$e 10 l)cine (a"ino acetic acid) in 100 "l La*orator) p(re -ater&
9& 5 3 s(lph(ric Acid@
Dil(te 5 "l o' Concentrated 82SO4 to 100 "l la*orator) p(re -ater&
;& SOD4>M B4CA?BO7A1E SOL>14O7@
Dissol$e 2;&5 o' Sodi(" Bi-Car*onate in 500 "l La*orator) p(re -ater&
<& SOD4>M A?SE741E SOL1>4O7@
(?eco""ended 'or 4nter'erence s(ppression i' needed) @
Dissol$e 0&5 o' 7aAsO2 in la*orator) p(re -ater and dil(te to 100 "l&
(CA>14O7 1OC4C ! ta.e care to a$oid inestion)
9& 184OACE1AM4DE SOL>14O7@
Dissol$e 0&250 o' C8/CS782 in 100 "l la*orator) p(re -ater&
(CA>14O7@ s(spected carcinoen& 1a.e care to a$oid s.in contact or inestion)
PROCED*RE:
CHLORINE DIOXIDE:
1& +lace 100 "l sa"ple in *ea.er&
2& Add 2 "l l)cine sol(tion&
/& +lace 5 "l o' phosphate *(''er in a 'las.&
4& Add 5 "l o' D+D reaent&
5& Add the sa"ple -ith l)cine to 'las. and "i%&
9& ?ead the a*sor*ance at 515 n" on spectrophoto"eter&
;& ?ecord as 6&
CALC*LATIONS:
Locate the a*sor*ances o' the sa"ple on the $ertical a%is o' the cali*ration c(r$e
and read the concentration on the hori2ontal a%is&
Con$ert all the a*sor*ances to concentration $al(es&
Chlorine Dio%ide D 56 ( or 1&96 e%pressed as ClO2)