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Determination of Chlorine Dioxide by DPD Method

This document provides the procedure for determining chlorine dioxide levels using the DPD (diethyl-p-phenylenediamine) method. It describes sample collection and storage requirements, potential interferences, necessary equipment, preparation of reagents, and step-by-step procedures for analyzing a sample and calculating chlorine dioxide concentration based on a calibration curve. Reagents include phosphate buffer solution, DPD indicator, potassium iodide, glycine solution, sulfuric acid, sodium bicarbonate, sodium arsenite, and thioacetamide solutions. The procedure involves adding sample, lye, buffer and DPD, mixing, and measuring absorbance at 515nm to locate the concentration on a calibration curve

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104 views3 pages

Determination of Chlorine Dioxide by DPD Method

This document provides the procedure for determining chlorine dioxide levels using the DPD (diethyl-p-phenylenediamine) method. It describes sample collection and storage requirements, potential interferences, necessary equipment, preparation of reagents, and step-by-step procedures for analyzing a sample and calculating chlorine dioxide concentration based on a calibration curve. Reagents include phosphate buffer solution, DPD indicator, potassium iodide, glycine solution, sulfuric acid, sodium bicarbonate, sodium arsenite, and thioacetamide solutions. The procedure involves adding sample, lye, buffer and DPD, mixing, and measuring absorbance at 515nm to locate the concentration on a calibration curve

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DETERMINATION OF CHLORINE DIOXIDE

BY DPD METHOD
Method Reference:
Standard Methods 19 th Editon (4500 CLO2-D)
Sam!e Conta"ner:
A clean plastic or lass container (250 ! 1000 "l#) a$oid e%cessi$e liht and aitation
On# S"te S$r%e& Re'$"rement(
1& Each '(ll) appro$ed anal)st "(st *e a*le to de"onstrate the constr(ction o' the
D+D cali*ration c(r$e&
2& All reaents, standards and sol(tions (sed 'or cali*ration and anal)sis -ill *e
chec.ed 'or proper la*elin, dates and records&
/& proper proced(ral techni0(e and interpretation o' data -ill *e o*ser$ed&
Interference(
1& 1(r*idit) and color "a) inter'ere -ith the deter"ination o' the a*sor*ance
contri*(tion 'ro" D+D&
2& O%idi2ed "ananese is the "ost sini'icant so(rce o' inter'erence( this t)picall)
is not a pro*le" in 'inished drin.in -ater)& 1o red(ce inter'erence, i' e%cess
"ananese is present, place 5 "l *(''er sol(tion and 0&5 "l sodi(" arsenite
sol(tion in 'las.& Add 100 "l sa"ple and reaents, read a*sor*ance and s(*tract
'ro" readins o*tained in "ethod& A 0&253 sol(tion o' thioaceta"ide can *e
s(*stit(ted 'ro the sodi(" arsenite&
/& 4nter'erence o' copper le$els (p to appro%i"atel) 10 "5L is o$erco"e *) the
ED1A in the D+D sol(tion& 1he ED1A also helps the sta*ilit) o' the D+D
sol(tion&
E'$"ment
1& Standard La*arator) 6lass-are&
2& Class A $ol("etric pipets or an accepta*le "icroliter pipettor 'or standardi2ations
/& A spectrophoto"eter capa*le o' readin 515 n" -ith a cell liht path -idth o'
atleast 1 c"&
4& 6raph paper or a co"p(ter -ith so't-are capa*le o' per'or"in linear
reressions&
Rea)ent(
1. PHOSPHATE BUFFER SOLUTION:
Dissol$e 24 ra"s o' anh)dro(s 7a28+O4 and 49 ra"s o' anh)dro(s :82+O4
in appro%i"atel) ;00 "l o' La*arator) p(re -ater&
Dissol$e 0&< ra"s o' disodi(" ED1A in 100 "l La*arator) p(re -ater
Co"*ine these t-o sol(tions
Dil(te to 1 litre -ith la*orator) p(re -ater
Add 0&0020 o' 8Cl2 as a preser$ati$e&
Store in a"*er lass&
2. DPD INDICATOR
Dissol$e 1&0 o' D+D o%alate, or 1&5 o' D+D s(l'ate pentah)drate, or 1&1 o'
anh)dro(s D+D s(l'ate in Chlorine 'ree la*orator) p(re -ater containin < "l
1=/ 82SO4 ( 9"l -ater = 2 "l 82SO4)
Dissol$e 0&2 o' disodi(" ED1A in the a*o$e sol(tion
Dil(te to 1 Litre and store in a"*er lass&
/& +O1ASS4>M +E?MA76A7A1E SOL>14O7S@
(See D+D Chlorine Method)
4& +O1ASS4>M 4OD4DE, :4 cr)stals
5& 6LAC47E SOL>14O7@
Dissol$e 10 l)cine (a"ino acetic acid) in 100 "l La*orator) p(re -ater&
9& 5 3 s(lph(ric Acid@
Dil(te 5 "l o' Concentrated 82SO4 to 100 "l la*orator) p(re -ater&
;& SOD4>M B4CA?BO7A1E SOL>14O7@
Dissol$e 2;&5 o' Sodi(" Bi-Car*onate in 500 "l La*orator) p(re -ater&
<& SOD4>M A?SE741E SOL1>4O7@
(?eco""ended 'or 4nter'erence s(ppression i' needed) @
Dissol$e 0&5 o' 7aAsO2 in la*orator) p(re -ater and dil(te to 100 "l&
(CA>14O7 1OC4C ! ta.e care to a$oid inestion)
9& 184OACE1AM4DE SOL>14O7@
Dissol$e 0&250 o' C8/CS782 in 100 "l la*orator) p(re -ater&
(CA>14O7@ s(spected carcinoen& 1a.e care to a$oid s.in contact or inestion)
PROCED*RE:
CHLORINE DIOXIDE:
1& +lace 100 "l sa"ple in *ea.er&
2& Add 2 "l l)cine sol(tion&
/& +lace 5 "l o' phosphate *(''er in a 'las.&
4& Add 5 "l o' D+D reaent&
5& Add the sa"ple -ith l)cine to 'las. and "i%&
9& ?ead the a*sor*ance at 515 n" on spectrophoto"eter&
;& ?ecord as 6&
CALC*LATIONS:
Locate the a*sor*ances o' the sa"ple on the $ertical a%is o' the cali*ration c(r$e
and read the concentration on the hori2ontal a%is&
Con$ert all the a*sor*ances to concentration $al(es&
Chlorine Dio%ide D 56 ( or 1&96 e%pressed as ClO2)

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