Tanin - JCPR 2012 4 6 3259 3262
Tanin - JCPR 2012 4 6 3259 3262
Tanin - JCPR 2012 4 6 3259 3262
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Research Article
Analysis of total phenols,total tannins and screening of phytocomponents in Indigofera aspalathoides (Shivanar Vembu) Vahl EX DC
N Tamilselvi1*, P Krishnamoorthy1, R Dhamotharan2, P Arumugam3 and E Sagadevan3
1 Department of Bioinformatics, Bharath University, Chennai, Tamilnadu, India P.G and Research, Dept of Plant Biology and Plant Biotechnology, Presidency College, Chennai-600005, Tamilnadu 3 Armats Biotek, 8/67, Ellaiammakoil Street, Kottur, Chennai-600 085, Tamilnadu _____________________________________________________________________________________________ 2
ABSTRACT The plant Indigofera aspalathoides is popularly known as Sivanar vembu in Tamil belongs to the family fabaceae. The stem is traditionally used for various skin disorders and cancer. In the traditional medicinal system, the leaves, flowers and tender shoot are said to be cooling and demulcent, they are used in the form of decoction for leprosy and cancer affections. Qualitative and quantitative Phytocomponents test were screened in Indigofera aspalathoides. The total content of some important phytochemical constituents like phenols, tannins of Indigofera aspalathoides by studied using spectrophotometric methods. The results showed that the plant is rich in phenols (47.381.532) compare than Tannins (34.591.788). KeyWords: Indigofera aspalathoides, Total phenol, Total Tannin, Screening of phytocomponents _____________________________________________________________________________________________ INTRODUCTION Natural antioxidants such as phenols, flavonoids and tannins are increasingly attracting because they are natural disease preventing, health promoting and anti-ageing substances [1]. Antioxidants may serve the task of reducing oxidative damage in humans induced by free radicals and reactive oxygen species under oxidative stress conditions. These conditions can cause DNA and protein damage, lipid peroxidation, cancer, ageing and inflammatory activity [2].Recently there has been an upsurge of interest in the therapeutic potentials of medicinal plants as antioxidants in reducing free radical induced tissue injury [3] . Phenolic compounds are secondary metabolites which synthesize in plants. They posses biological properties such as: antioxidant, anti apoptosis, anti-aging, anti carcinogen, anti-inflammation, anti-atherosclerosis, cardiovascular protection, improvement of the endothelial function, as well as inhibition of angiogenesis and cell proliferation activity. Most of these biological actions have been attributed to their intrinsic reducing capabilities [4]. Plant foods have phenolic compounds, which affect their appearance, taste, odor and oxidative Stability. In cereal grains, these compounds are located mainly in the pericarp[5].The major phenolic acids in cereals are ferulic and pcoumaric acids[6,7,8,9]. Anthocyanins are water-soluble pigments mostly studied in cereals [10].The tannins are widely distributed in almost all plant foods [11] species [12]. According to World Health Organization more than 21 000 plants are being in use for medicinal purpose all around the world [13]. Numbers of plants were screened for primary and secondary metabolites for their medicinal values [14,15,16,]
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Total Phenol and Tannins The results of total phenol content, tannins and are given table1. Total phenolic content was found to be more in methanol extract followed by tannin(Table2). Phenolic and polyphenols like flavonoid are secondary plant metabolites that are ubiquitously present in plants. The values presented as the mean SD of three measurements.
Table -2 Bioactive compounds in Methanol extract of Indigofera aspalathoides Bioactive compounds Total phenol Tannin Indigofera aspalathoides (Methanol extract) 47.381.532 34.591.788
It is well-known that phenolic compounds contribute to quality and nutritional value in terms of modifying color,taste, aroma, and flavor and also in providing health beneficial effects. They also serve in plant defense
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