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    CAT Nº: 1033: Standard Methods Agar With Powdered Milk

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    Abdo Hanafy
    Standard Methods Agar with Powdered Milk is a culture medium used to enumerate microorganisms in dairy products using pour plate or surface inoculation techniques. The medium contains tryptone, yeast extract, dextrose, skimmed milk powder, and bacteriological agar. To prepare, the powder is suspended in water and boiled before being dispensed into plates and autoclaved. The medium supports growth of Escherichia coli, Staphylococcus aureus, and Staphylococcus epidermidis from dairy samples and is used to calculate bacterial colony forming units per milliliter after incubation.

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    CAT Nº: 1033: Standard Methods Agar With Powdered Milk

    Uploaded by

    Abdo Hanafy
    23 views2 pages
    Standard Methods Agar with Powdered Milk is a culture medium used to enumerate microorganisms in dairy products using pour plate or surface inoculation techniques. The medium contains tryptone, yeast extract, dextrose, skimmed milk powder, and bacteriological agar. To prepare, the powder is suspended in water and boiled before being dispensed into plates and autoclaved. The medium supports growth of Escherichia coli, Staphylococcus aureus, and Staphylococcus epidermidis from dairy samples and is used to calculate bacterial colony forming units per milliliter after incubation.

    Original Description:

    skimed milk powder agar (media)

    Original Title

    1033

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    © © All Rights Reserved

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    Standard Methods Agar with Powdered Milk is a culture medium used to enumerate microorganisms in dairy products using pour plate or surface inoculation techniques. The medium contains tryptone, yeast extract, dextrose, skimmed milk powder, and bacteriological agar. To prepare, the powder is suspended in water and boiled before being dispensed into plates and autoclaved. The medium supports growth of Escherichia coli, Staphylococcus aureus, and Staphylococcus epidermidis from dairy samples and is used to calculate bacterial colony forming units per milliliter after incubation.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    23 views2 pages

    CAT Nº: 1033: Standard Methods Agar With Powdered Milk

    Uploaded by

    Abdo Hanafy
    Standard Methods Agar with Powdered Milk is a culture medium used to enumerate microorganisms in dairy products using pour plate or surface inoculation techniques. The medium contains tryptone, yeast extract, dextrose, skimmed milk powder, and bacteriological agar. To prepare, the powder is suspended in water and boiled before being dispensed into plates and autoclaved. The medium supports growth of Escherichia coli, Staphylococcus aureus, and Staphylococcus epidermidis from dairy samples and is used to calculate bacterial colony forming units per milliliter after incubation.

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    © All Rights Reserved
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    of 2

    STANDARD METHODS AGAR WITH POWDERED MILK

    CAT N: 1033
    For bacterial plate counts of microorganisms from milk and dairy derivatives APHA* Formula

    FORMULA IN g/l
    Tryptone Dextrose Bacterilogical agar 5.00 1.00 15.0 Yeast extract Skimmed milk powder 2.50 1.00

    Final pH 7.0 0.2 at 25C

    PREPARATION
    Suspend 24.5 grams of medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121C for 15 minutes. Cool to 50, mix well and dispense into plates. The color of the prepared medium is clear amber, slightly opalescent. The dehydrated medium should be homogeneous, free-flowing and beige in color. If there are any physical changes, discard the medium. * APHA: American Public Health Association Inc.

    USES
    STANDARD METHODS AGAR WITH POWDERED MILK is used for enumerating microorganisms in dairy products. It is used with the same techniques as the Standard Methods Agar. Tryptone is a source of nitrogen, vitamins and minerals; Yeast extract provides vitamins especially the B-group. Dextrose is a source of carbohydrate as energy source. Skimmed milk is a source of lactose and casein. Bacteriological agar is the solidifying agent. Dispense a portion of each test dilution (e.g., 0.1, 1.0 ml) into separate sterile Petri dishes. Add 10 12 ml of tempered (45C) Standard Methods Agar with Powdered Milk to Petri dishes containing test dilutions. Swirl the dishes to thoroughly mix the agar and test dilution. Allow plates to cool and solidify. Incubate at 35 2C for 18-24 hours. APHA recommends the use of pour plate technique with this media. In general, 1 ml of the appropriate sample test dilution is added to the sterile medium at a temperature of 44 45C, mixed gently and poured into sterile Petri dishes. Sterile molten agar is added to these plates and plates are rotated gently to ensure uniform mixing of the sample with agar. Allow plates to cool and solidify. The poured plate count method is preferred to the surface inoculation method, since it gives higher results. Plate Count Agar is also suitable for enumerating bacterial count of sterile. Count colonies on all plates containing 30 300 colonies. Calculate bacterial count per milliliter of the sample by multiplying the average number of colonies per plate by the reciprocal of the dilution used. Report the count as CFU/ml. Incubate the Petri dishes at 35 2C for 18 24 hours and count the developed colonies.

    1 LABORATORIOS CONDA, S.A.

    www.condalab.com

    MICROBIOLOGICAL TEST
    The following results were obtained in the performance of the medium from type cultures after incubation at a temperature of 35 2C and observed after 18-24 hours
    Microorganisms Escherichia coli ATCC 25922 Staphylococcus aureus ATCC 25923 Staphylococcus epidermidis ATCC 12228 Growth Good Good Good

    BIBLIOGRAPHY
    R.C. MARSHALL (1.993) Standard Methods for the Microbiological examination of dairy products, 16th Ed. (American Public Health Association, Washington, D.C.). England and Wales. The Dairy Products (Hygiene) Regulations 1995 Statutory Instrument No. 1086. London: HMSO, 1995. British Standards Institution. BS 4285 Microbiological examination for dairy purposes. Section 2.1 Enumeration of microorganisms by poured plate technique for colony count. London: BSI, 1984.

    STORAGE
    Once opened keep powdered medium closed to avoid hydration.
    2C

    25C

    2 LABORATORIOS CONDA, S.A.

    www.condalab.com

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