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The document contains data and instructions for multiple chemistry experiments involving spectrophotometric analysis. The experiments determine concentrations of various metals and acids using calibration curves, complex formation reactions, and acid-base titrations monitored via absorbance measurements. Key details include: 1) A multiple standard addition method is used to determine the Fe2+ concentration in a water sample. Absorbances are measured for solutions with varying additions of Fe2+ standard and the concentration is calculated. 2) Data is provided to determine the formula of complexes formed between Fe2+ and o-phenanthroline and between Vn+ and Sn2+. Absorbances are measured for solutions with varying metal to ligand ratios. 3

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249 views4 pages

Assignment

The document contains data and instructions for multiple chemistry experiments involving spectrophotometric analysis. The experiments determine concentrations of various metals and acids using calibration curves, complex formation reactions, and acid-base titrations monitored via absorbance measurements. Key details include: 1) A multiple standard addition method is used to determine the Fe2+ concentration in a water sample. Absorbances are measured for solutions with varying additions of Fe2+ standard and the concentration is calculated. 2) Data is provided to determine the formula of complexes formed between Fe2+ and o-phenanthroline and between Vn+ and Sn2+. Absorbances are measured for solutions with varying metal to ligand ratios. 3

Uploaded by

Siva Sree Hari
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOC, PDF, TXT or read online on Scribd
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EKC 108 TUTORIAL 2013/2014 1. The determination of Fe in an industrial waste stream was carried out by the o-phenanthroline.

Using the data shown in the following table, determine the concentration of Fe in the waste stream. ppm Fe 0.00 1.00 !.00 .00 #.00 un&nown Absorbance 0.000 0.18 0. "# 0.$#" 0.%!% 0.!"'

!. A multiple standard addition method was used for determining Fe ( in a natural water sample. 10 m) ali*uots of the sample were pipetted into $0.m) +olumetric flas&s. ,-actly 0.00, $.00, 10.00, 1$.00 ad !0.00 m) of standard solution containing 11.1 ppm of Fe ( were added to each followed by an e-cess of thiocyanate ion to gi+e the red comple- Fe./012!(. After dilution to +olume, absorbances of the fi+e solutions were measured in a 0.'8! cm cell at #80 nm and found to be 0.!#0, 0.# %, 0."!1, 0.80' and 1.00' respecti+ely. 3hat is the concentration of Fe ( in the water sample4 . The concentration of Titanium and +anadium was determined through the formation of 5!6! comple-. The absorbances of mi-ed solution Ti and 7 and standard solution " .1 ppm Ti and '".# ppm 7 is gi+en below8 3a+elength '0 # 0 #$0 #%0 $10 /td Ti 0.8'$ 0.88# 0."'# 0.#81 0.1% Absorbances /td 7 9i-ed /olution 0. !" 0."$1 0.#'% 0.%# 0.$!8 0.""$ 0.$1! 0.$#% 0. %# 0. 1#

0alculate the concentration for Ti and 7 in the mi-ed solution. #. To determine the formula for the comple- between Fe!( and ophenantroline, a series of solution was prepared in which the total concentration of metal and ligand was held constant at .1$ - 10-# 9. The

absorbance of each solution was measured at a wa+elength of $10 nm. Using the following data, determine the formula for the comple-. :) 0.0 0.1 0.! 0. 0.# 0.$ Absorbance 0.000 0.11" 0.! 1 0. #% 0.#"! 0.$%8 :) 0." 0.% 0.8 0.' 1.0 Absorbance 0."' 0.80' 0."' 0. #% 0.000

". The formula of 0omple- 9:n can be determined. To # m) portions of a 0.01 9 solution of 9 are added +arying +olumes of 0.019 solution of :, and the solutions are diluted to !0 m) with an appropriate bac&ground solution. The data are shown below 1o 1 ! m) of : 0 ! absorbances 0 0.1!$

# $ " % 8 ' 10 11 1! 1

# $ % 8 ' 11 1! 1 1# 1$

0.18$ 0.!#8 0. 10 0.# $ 0.#'8 0.$$' 0."8$ 0.% 8 0.%%1 0.%%$ 0.%%$

%. The acidity constant for an acid-base indicator was determined by preparing solutions, each of which has a total indicator conc of $ - 10-$ 9. The first solution was made strongly acidic with 50l and has an absorbance of 0.!$. The second solution was made strongly basic with absorbance of 1.#. The p5 of the third solution was measured at !.'1 with an abs at 0.""!. 3hat is the +alue of ;a for the indicator4 8. 3hy is a calibration cur+e li&ely to be linear o+er a wider range of concentrations at the wa+elength of ma-imum absorption compared to a wa+elength on a shoulder of the absorption cur+e4 '. 10. 11. <istinguish between a single beam and a double beam spectrophotometer. <escribe the factors that cause the =eer>s law relationship to depart from linearity. A second instrumental limitation to =eer>s )aw is stray radiation. The following data were obtained using a cell with a pathlength of 1.00 cm, when the stray light is insignificant .?stray @ 02. [Analyte] (M) 0.000 0.00! 0.00# 0.00" 0.008 0.010 Absorbance 0.00 0.#0 0.80 1.!0 1."0 !.00

0alculate the absorbance of each solution when ? stray is $A of ?0 and plot beer>s law calibration cur+es for both sets of data. ,-plain any differences between the two cur+es. .5int8 Assume that ?0 is 1002 1!. A mixture of MnO4- and Cr2O72- and standards of 1.0 x 10-4 M KMnO4 and 1.0 x 10-4 M K2Cr2O7 gave the following results !avelength "nm# 2'' 2(( )20 )*0 )'0 MnO4- &tandard 0.042 0.0(2 0.1'( 0.12* 0.0*' A$sor$an%es Cr2O72- &tandard 0.410 0.2() 0.1*( 0.)1( 0.1(1 Mixture 0.7'' 0.*71 0.422 0.'72 0.)''

+etermine the molar %on%entration of ea%h anal,te in the mixture.

1 . The acidity constant for an organic wea& acid was determined by measuring a constant total concentration of the acid. Using the data from table. <etermine the acidity constant for the organic wea& acid. p5 1.$ !.!0 ."" #.11 #. $ #.%$ #.88 $.0' $."' %.!0 %.%8 Absorbance 0.010 0.010 0.0 $ 0.0%! 0.10 0.1"' 0.1' 0.!!% 0.!88 0. 1% 0. 1%

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