Republic of the Philippines

DEPARTMENT OF AGRICULTURE BUREAU OF PLANT INDUSTRY

PLANT VARIETY PROTECTION OFFICE

GUIDELINES FOR THE CONDUCT OF TESTS FOR DISTINCTNESS, UNIFORMITY AND STABILITY

ONION and SHALLOT

TABLE OF CONTENTS

I. II. III. IV. V. VI. VII. VIII. IX. X.

Subject of these Guidelines Material Required Conduct of Tests Methods and Observations Grouping of Varieties Characteristics and Symbols Table of Characteristics

.3 .3 .3 .3-4 .4 .4 ...5-13

Explanations on the Table of Characteristics .14-19 Literature Technical Questionnaire .20-21 .22-26

I.

Subject of these Guidelines

These Test Guidelines apply to all seed and vegetatively propagated varieties of onion (Allium cepa L.) and multiplier onion or shallot (A. cepa var. ascalonicum) II. Material Required

1. The Plant Variety Protection Office (PVPO) decides where and in what quantity and quality the plant material required for testing the variety is to be delivered. Applicants submitting material outside the Philippines must make sure all customs formalities are complied with. The minimum quantity of plant material or seed to be supplied by the applicant in one or several samples should be: (a) (b) Seed propagated varieties: 30g of seed Vegetatively propagated varieties: 500 bulblets or sets

The seeds submitted for testing must have at least 90% germination, utmost 10% moisture content and 99% purity. 2. The seed and plant material must not have undergone any treatment unless the PVPO requires such treatment. If it has been treated, full details of the treatment must be given. III. Conduct of Tests

2. A minimum of two tests will be required and these be conducted during the dry season at one place. If any important characteristics of the variety cannot be seen at that place, the variety may be tested at an additional location. 3. The tests should be carried out under conditions ensuring normal growth. The size of the plots should be 1 x 5 m. or 10 sq. m. for the two replications following 20 cm x 20 cm planting distance. Each test should include a total of 250 plants. Separate plots for observation and for measuring can only be used if they have been subject to similar environmental conditions. 4. IV. Additional tests for special purposes may be established.

Methods and Observations

1. All observations determined by measurement or counting should be made on 60 plants or parts taken from each of 60 plants. 2. For the assessment of uniformity of vegetatively propagated varieties, a population standard of 1% with an acceptance probability of at least 95% should be applied. In the case of a sample size of 100 plants the maximum number of off-types allowed would be 3. 3. For the assessment of uniformity of open-pollinated and hybrid varieties relative uniformity standards should be applied. 4. All observations on the leaf and the foliage should be made on the fifth leaf stage.
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5. V.

All observations on the bulb should be made at harvest maturity.

Grouping of Varieties

1. The collection of varieties to be grown should be divided into groups to facilitate the assessment of distinctness. Characteristics which are suitable for growing purposes are those which are know from experience not to vary, or to vary only slightly, within a variety. Their various stage of expression should be fairly distributed throughout the collection. 2. It is recommended that the following characteristics will be used for grouping varieties: (a) (b) (c) VI. Bulb: splitting into bulblets (with dry skin around each bulblet) (characteristic 10) Bulb/Bulblet: general shape (in longitudinal section) (characteristic 18) Bulb/Bulblet: basic color for dry skin ( characteristic 23)

Characteristics and Symbols

1. To assess distinctness, uniformity and stability, the characteristics and their states as given in the Table of Characteristics should be used. The name of each example variety is followed by an abbreviation indicating whether the variety is an onion (O) or a shallot (S). 2. Notes (numbers), for purposes of electronic data processing, are given opposite the states of expression for each characteristic. 3. Legend: (*) Characteristics that should be used on all varieties in every growing period over which the examinations are made and always be included in the variety descriptions, except when the state of expression of a preceding characteristics or regional environmental conditions render this impossible. (+) See Explanations on the Table of Characteristics in Chapter VIII. (1) The optimum stage of development for the assessment of each characteristic is indicated by a number in the second column. The stages of development denoted by each number are described at the end of the Chapter VIII. (2) Type of example variety: O = Onion, S = Shallot.

VII. Trait No. 1. (*)

Table of Characteristics Characteristic Description/ Category few Medium Red Creole, Red Ping, Yellow Granex, Red Pinoy, Nueventa Tanduyong, Batanes, Farmers Batanes Red Pinoy, Nueventa Red Creole, Red Ping Tanduyong, Batanes, Farmers Batanes, Reference (Example) Varieties ONION SHALLOT Note/ Remarks 3 5

Plant: number of leaves per pseudostem

Many

2 (*)

Foliage: orientation

Erect

semi-erect horizontal 3 (*) Foliage: waxiness absent

2 3 Tanduyong, Batanes, Farmers Batanes 1

medium Strong 4. (*) Foliage: green color Light medium

Red Creole, Red Ping Red Pinoy, Nueventa

5 9 3 Batanes Red, Tanduyong, Farmers Batanes 5

Dark

Red Pinoy, Red Creole, Red Pinoy, Nueventa Tanduyong, Batanes Red Pinoy, Yellow Granex Red Creole, Red Pinoy, Nueventa Farmers Batanes

5. (* ) (+)

Foliage: cranking

absent or very weak weak

medium

Trait No.

Characteristic

Description/ Category Strong very strong

Reference (Example) Varieties ONION SHALLOT

Note/ Remarks 7 9 1

6.1

Onion varieties only: Leaf: length

very short short Medium Red Pinoy Red Creole, Red Pinoy, Nueventa Yellow Granex

3 5

long very long 6.2 Shallot varieties only: Leaf: length very short short medium long very long 7.1 (*) Onion varieties only: Leaf: diameter Small medium

7 9 1 Tanduyong Batanes, Farmers Batanes 3 5 7 9

Red Pinoy Red Creole, Red Pinoy, Nueventa Yellow Granex Tanduyong, Farmers Batanes Batanes Red

3 5

large 7.2 (*) Shallot varieties only: Leaf: diameter Small

7 3

medium Large

5 7

Trait No. 8. (+)

Characteristic

Description/ Category Short medium

Onion varieties only: Pseudostem: length (up to highest green leaf)

Reference (Example) Varieties ONION SHALLOT Red Pinoy Red Creole, Red Pinoy, Nueventa Yellow Granex Red Pinoy, Red Pinoy, Nueventa Red Creole Yellow Granex Yellow Granex, Red Pinoy, Nueventa Red Pinoy Yellow Granex Red Creole, Red Pinoy, Red Pinoy, Nueventa

Note/ Remarks 3 5

long 9. (+) Onion varieties only: Pseudostem: diameter (at midpoint of length) Small medium Large 10.a (*) (+) Bulb:splitting into bulblet (with dry skin around each bulblet) Absent

7 3 5 7 1

present 10.b (*) Bulb: internal split Absent present

9 1 9

11 (*)

Shallot varieties only: Bulb: degree of splitting into bulblet

weak (1-2) medium (3-4) strong ( more than 5) Batanes Red Tanduyong Red Pinoy Red Creole Yellow Granex Sibuyas Tagalog Tanduyong Batanes

3 5 7 3 5 7 3 5 7

12.a (*)

Onion varieties only: Bulb: size

Small medium large

12.b (*)

Shallot varieties grown from bulblets only: Bulblet: size

Small medium large

Trait No. 13.a (*)

Characteristic

Description/ Category short medium

Onion varieties only: bulb Polar diameter

Reference (Example) Varieties ONION SHALLOT Red Pinoy Red Creole, Red Pinoy, Nueventa Yellow Granex Sibuyas Tagalog Batanes Tanduyong Red Pinoy Red Creole, Red Pinoy, Nueventa Yellow Granex Sibuyas Tagalog, Farmers Batanes Tanduyong Batanes Red Pinoy Red Creole, Red Pinoy, Nueventa Yellow Granex Sibuyas Tagalog, Farmers Batanes Batanes Tanduyong

Note/ Remarks 3 5

tall 13.b (*) Shallot varieties grown from bulblets only: Bulblet: polar diameter short medium tall 14.a (*) Onion varieties only: Bulb: equatorial diameter small medium

7 3 5 7 3 5

large 14.b (*) Shallot varieties grown from bulblet only: Bulblet: equatorial diameter small medium large 15.a (*) Onion varieties only: Bulb:ratio height/diameter small medium

7 3 5 7 3 5

large 15.b (*) Shallot varieties from Bulblet only: Bulblet: Ratio height/diameter small

7 3

medium large

5 7

Trait No. 16 (*) (+)

Characteristic

Description/ Category towards apex at middle towards base

Reference (Example) Varieties ONION SHALLOT

Note/ Remarks 1

Bulb/Bulblet: position of maximum diameter

Red Creole, Yellow Granex Red Pinoy, Red Pinoy, Nueventa Red Pinoy, Nueventa Red Creole Yellow Granex Farmers Batanes

2 3

17 (+)

Bulb/Bulblet: width of neck

narrow medium broad

3 5 7 1 2 3

18 (*) (+)

Bulb/Bulblet: general shape (in longitudinal section)

elliptic ovate broad elliptic circular broad ovate broad obovate rhombic transverse elliptic transverse narrow elliptic Red Pinoy Yellow Granex Red Creole

4 5 6 7 8 9

19. (*) (+)

Onion varieties only: Bulb: shape of top (as for 18) Depressed

flat slightly raised rounded slightly sloping strongly sloping Red Pinoy, Nueventa Red Creole, Red Pinoy Yellow Granex

1 2 3 4 5

Trait No. 20. (*) (+)

Characteristic

Description/ Category recessed flat round weakly tapered strongly tapered

Reference (Example) Varieties ONION SHALLOT

Note/ Remarks 1

Bulb/Bulblet: shape of base (as for 18)

Red Pinoy, Yellow Granex, Red Creole Red Pinoy, Nueventa

Farmers Batanes

2 3 4 5

21.

Bulb/Bulblet: adherence of dry skin after harvest

weak medium strong

Yellow Granex Red Creole, Red Pinoy, Red Pinoy, Nueventa Yellow Granex Red Creole Red Pinoy, Nueventa Farmers Batanes Farmers Batanes

3 5 7

22.

Bulb/Bulblet: thickness of dry skin

thin medium thick

3 5 7

23. (*)

Bulb/Bulblet: basic color of dry skin

white gray green yellow brown pink red Red Creole, Red Pinoy, Nueventa Yellow Granex Farmers Batanes

1 2 3 4 5 6 7

10

Trait No. 24. (*)

Characteristic

Description/ Category light medium dark

Bulb/Bulblet: intensity of basic color of dry skin

Reference (Example) Varieties ONION SHALLOT Yellow Granex Farmers Batanes Red Creole, Red Pinoy, Nueventa

Note/ Remarks 3 5 7

25. (*)

Bulb/Bulblet: hue of color of dry skin (in addition to basic color)

absent grayish greenish yellowish brownish pinkish reddish Red Creole, Red Pinoy, Nueventa Farmers Batanes, Yellow Granex

1 2 3 4 5 6 7

purplish 26. (*) Bulb/Bulblet: coloration of epidermis of fleshy scales absent greenish reddish Red Creole, Red Pinoy, Nueventa Farmers Batanes Yellow Granex

8 1 2 3

27. (*) (+)

Bulb/Bulblet: number of axes

very few few Yellow Granex, Red Pinoy, Nueventa Red Creole, Red Pinoy Farmers Batanes

1 3

medium many very many

5 7 9

11

Trait No. 28. (+)

Characteristic

Description/ Category very low low medium high

Reference (Example) Varieties ONION SHALLOT

Note/ Remarks 1

Bulb/Bulblet: dry matter content

Yellow Granex

3 5

Red Creole, Red Ping, Red Pinoy, Nueventa

Farmers Batanes

very high 29. Onion: tendency to bolt absent or very weak Yellow Granex, Red Pinoy, Nueventa Red Pinoy, Red Creole

9 1

weak

medium strong very strong 30. Onion varieties only: time of beginning of bolting early medium late 31.a Onion varieties only: time of harvest maturity early medium Yellow Granex Red Pinoy, Red Creole Red Pinoy, Nueventa Yellow Granex. Nueventa Red Pinoy, Red Creole,

5 7 9 3 5 7 3 5

late

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Trait No. 31.b

Characteristic

Description/ Category early medium late

Shallot varieties only: time of harvest maturity

Reference (Example) Varieties ONION SHALLOT Australia, Farmers Batanes Tanduyong Batanes Batanes Tanduyong Red Pinoy, , Nueventa Farmers Batanes

Note/ Remarks 3 5 7 3 5 7

32. (*)

Time of sprouting during storage

early medium late

33. 33.1.

Reaction to Pest Purple blotch

susceptible

Red Pinoy, Nueventa 6 S (Sumenep) Red Pinoy, Nueventa

Farmers Batanes

resistant 33.2. Cercospora leaf spot susceptible resistant 33.3. Bulb rot susceptible resistant 33.4. Anthracnose susceptible resistant 33.5 Nematode susceptible resistant

Farmers Batanes

Red Pinoy, Nueventa

Farmers Batanes

Red Pinoy, Nueventa

Farmers Batanes

Red Pinoy, Nueventa

Farmers Batanes

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VIII.

Explanations on the Table of Characteristics

Trait No. 5: Foliage: cranking

1 absent or very weak

3 weak

5 medium

7 strong

9 very strong

Trait No. 8 and 9 Onion varieties only: pseudostem: length (up to highest green leaf) (8) and diameter (at midpoint of length) (9)

Trait No. 10 Bulb: splitting into bulblets (with dry skin around each bulblet)

1 absent

9 present

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Trait No. 16: Bulb/Bulblet: position of maximum diameter

1 towards apex

2 at middle

3 towards base

Trait No. 17: Bulb/Bulblet: width of neck

1 very narrow

3 narrow

5 medium

7 broad

9 very broad

Trait No. 18: Bulb/Bulblet: general shape ( in longitudinal section)

1 elliptic

2 ovate

3 broad elliptic

4 circular

5 broad ovate

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6 broad obovate

7 rhombic

8 tranverse elliptic

9 tranverse narrow elliptic

Ad. 19: Onion varieties only: Bulb: shape of top (as for 18)

2 flat

1 depressed

3 slightly raised

4 rounded

5 slightly sloping

6 strongly sloping

Ad. 20: Bulb/Bulblet: shape of base (as for 18)

1 recessed

2 flat

3 round

4 weakly tapered

5 strongly tapered

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Ad. 27: Bulb/Bulblet: number of axes T he number of axes should be assessed when the bulb/bulblet has completely died back and before there is any sprouting. The bulb or bulblet should be cut in transverse section, starting at the widest point and make further cuts towards the base of the bulb/bulblet, until there is consistency in the number of axes. Each axis appears as a point, often greenish in color surrounded by tissue rings. Add. 28: Bulb/Bulblet: dry matter content Dry matter content should be determined according to IV-I for 3 x 20 bulbs (e.g. one sample of 20 bulbs from each plot). From these bulbs the dry skin should be prepared by cutting the bulbs into small pieces of 1-5 mm size. A representative sample should be weighed directly after cutting ( we should be aware that the biodegradation of sugars and carbohydrates starts as soon as cells are damaged). The sample should be dried for 2 hours at 1050C and then the temperature should be lowered to 650C during 22 hours. Lowering of temperature is necessary to avoid caramelisation. The remaining weight should be assed after 24 hours. From these figures the dry matter content may be calculated. The dry matter content could also be assessed by obtaining the total soluble solids (TSS) using a refractometer.

Ad. 35: Time of sprouting during storage Care should be taken to exclude damaged bulbs. Storage temperature should be maintained under ambient condition.

Code for the Growth Stages 1: Seed to Bulb Growth stage General description

Vegetative Cycle 00 Dry 0 Germination

Seedling growth 10 15 20 25 30 35 40 Emerged seedling at loop stage Seedling with testa above ground and still attached to cotyledon Emergence of first true leaf Second true leaf stage Third leaf stage Fourth leaf stage Fifth leaf stage

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Plant Growth 45 50 55 60 65 70 75 80 85 90 95 100 105 115 120 125 130 135 140 145 150 Sixth leaf stage Seventh leaf stage-first leaf senescing Eighth leaf stage Tenth leaf stage; second and third leaf senescing; early bulb development

Full expansion of leaves achieved; continued swelling of bulb Beginning of foliage fall-over, weakening of false stem turgidity Leaves dry off; bulb size continues to increase; darkening of bulb scales

Bulb at harvestable maturity

Complete die-back of foliage; bulb top seals over for dormancy

Reproductive Cycle 160.1 170.1 180.1 190.1 200.1 210.1 220.1 230.1 240.1 250.1 260.1 270.1 280.1 290.1 300.1 310.1 320.1 330.1 Start of sprouting in store swelling of root initials or emergence of shoot at top of bulb Sprouted bulbs with emerging leaves Splitting of outer skin Emergence of scape and underdeveloped spathe Scape elongation and swelling of middle Swelling of spathe Splitting of spade Enlargement of umbel Opening of florets (where fertile) Pollination of florets Seed set swelling of pollinated ovaries

Ripe seed in umbels

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340.1 340.1 350.1 150 160.2 170.2 180.2 190.2 200.2 210.2 220.2 230.2 240.2 250.2 260.2 270.2 280.2 290.2 300.2

Dry seed Dry bulb with complete foliage die-back Bulb shape becoming less rounded Bulb shape becoming irregular with development of small splits in the dry outer skin More than one growing point emerging from the top of the bulb Long splits developing in the outer skin of the bulb and differentiation of bulb into bulblets

Separation ob bulblets from parent bulb except at base. Bulblets separated from each other by a dry outer skin Complete separation of bulblets from parent bulb Development of many leaves

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IX. -

Literature BREWSTER, J. L., 1994: Crop Production Science in Horticulture 3: Onions and Other Vegetables Alliums. CAB International. BREWSTER, J. L. and BARNES, A., 1981: A Comparison of Relative Growth Rates of Different Individual Plants and Different Cultivars of Onion of Diverse Geographic Origin at Two Temperatures and Two Light Intensities. Journal of Applied Ecology Vol. 18, 589-604. BREWSTER, J. L., SALTER, P. J. and DARBY, R. J., 1977: Analysis of the Growth and Yield of Over-wintered Onions. Journal of Horticulture Science Vol. 52, 335-346. CLARKE, A. E., JONES, H. A. and LITTLE, T. M., 1994: Inheritance of Bulb Colour in the Onion. Genetics 29, pp 569-575. EL-SHAFIE, M. W. and DAVIES, G. N., 1967: Inheritance of Bulb Color in the Onion (Allium cepa L.). Hilgardia Vol. 38, No. 17, 607-622. JONES, H. A., CLARKE, A. E. and STEVENSON, F. J., 1944: Studies in the Genetics of the Onion (Allium cepa L.). Proceedings of the American Society for Horticulture Science 44, pp 479-484. JONES, H. A. and MANN, L. K., 1963: onions and Their Allies: Botany, Cultivation and Utilisation. London, Leonard Hill. JONES, H. A. and PETERSON, C. E., 1952: Complementary Factors for Light-Red Bulb Colour in Onions. Proceedings of the American Society for Horticulture Science Vol. 59, 457. KAPPERT and RUDOLF, W., 1962: Zchtung von Gemse, Obst, Reben and Forstpflanzen. Verlag Paul Parey, Berlin und Hamburg, pp 270-312. KUCKUCK, H. and KOBAKE, G., 1962, in ROEMER, T. and RUDOLF, W., 1962: Handbuch der Pfanzen-Zchtung, Band VI. Verlag Paul Parey, Berlin und Hamburg. MAGRUDER, R. and ALLARD, H. A., 1937: Bulb Formation in Some American and European Varieties of Onions as Affected by Length of Day. Journal of Agricultural Research Vol. 54, Part No. 10, 719-752. MAGRUDER, R. et al, 1941: Descriptions and Types of Principal American Varieties of Onion. USDA, Miscellaneous Publication No. 435, Washington DC. MESSIAEN, C. M., COHAT, L., LEROUX, J. P., PICHON, M., BEYRIES, A., 1993: Les allium alimentaires reproduits para voie vgtative. INRA Editions, Paris. MIDMORE, D. J., 1994, (Editor): International Symposium on Alliums for the Tropics. Acta Horticulturae, 358.

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RABINOWITCH, H. D. and BREWSTER, J. L., 1990: Onions and Allied Crops. Vol. 1-3 CRC Press, Boca Raton. REIEMAN, G. H., 1931: Genetic Factors for Pigmentation in the Onion and Their Relation to Disease Resistance. Journal of Agricultural Research Vol. 42, No. 5, 251-278. SCULLY, N. J., PARKER, M. W. and BORTHWICK, H. A. 1945: Interaction of Nitrogen Nutrition and Photo-period as Expressed in Bulbing and Flower Stalk Development of Onion. Botanical Gazette Vol. 107, 52-61. SCWARTZ, H. F. and MOHAN, S. K., 1995 (Editors): Compendium of Onion and Garlic Diseases. The American Phytopathological Society, (ISBN: 0-89-54-170-1). WRIGHT, C. J. and SOBEIGH, W. Y., 1986: The Photo-periodic Regulation of Bulbing in Onions (Allium cepa L.), Effects of Irradiance. Journal of Horticulture Science Vol. 61, Part 3, 311-335.

21

X.

Technical Questionnaire Reference Number (not to be filled in by the applicant) _____________________________ _____________________________

TECHNICAL QUESTIONNAIRE to be completed in connection with an application for plant breeders rights

1.

Species

Allium cepa L. Allium escalonicum L. (a) (b) ONION SHALLOT ( ( ) )

2.

Applicant (Name and address)

3.

Proposed denomination or breeders reference

4.

Information on origin, maintenance and reproduction of the variety 4.1 Variety type (a) (b) (c) (d) (e) 4.2 open-pollinated single hybrid three-way hybrid clone other type (indicate what type) ( ( ( ( ( ) ) ) ) )

Other information

22

5.

Characteristics of the variety to be indicated ( the number in brackets refers to the corresponding characteristic in Test Guidelines; please mark the state of expression which best corresponds). Characteristics Description/Category few medium many Example Varieties Note 3[ ] 5[ ] 7[ ] 3[ ] 5[ ] 7[ ] 1[ ] 9[ ]

5.1 (1)

Plant: number of leaves per pseudostem

5.2 (4)

Foliage: green color

light medium dark

5.3a (10)

Bulb: splitting into bulblets (with dry skin around each bulblet)

absent present

5.3b

Bulb: internal split

absent present

1[ ] 9[ ] 3[ ] 5[ ] 7[ ] 3[ ] 5[ ] 7[ ] 1[ ] 2[ ] 3[ ] 4[ ]

5.4.1 (12.1)

Onion varieties only: Bulb: size

small medium large

5.4.2 (12.2)

Shallow varieties grown from blblets only: Bulblet: size

small medium large

5.5 (18)

Bulb/Bulblet: general shape (in longitudinal section)

elliptic ovate broad elliptic circular

23

Characteristics

Description/Category broad ovate broad obovate rhombic transverse elliptic transverse narrow elliptic

Example Varieties

Note 5[ ] 6[ ] 7[ ] 8[ ] 9[ ]

5.6 (23)

Bulb/Bulblet: basic color of dry skin

white grey green yellow brown pink red

1[ ] 2[ ] 3[ ] 4[ ] 5[ ] 6[ ] 7[ ] 1[ ] 2[ ] 3[ ] 4[ ] 5[ ] 6[ ] 7[ ] 8[ ]

5.7 (25)

Bulb/bulblet: hue of color of dry skin

absent grayish greenish yellowish brownish pinkish reddish purplish

24

Characteristics 5.8 (33) Onion varieties only: Time of harvest maturity

Description/Category very early early medium late very late

Example Varieties

Note 1[ ] 2[ ] 3[ ] 4[ ] 5[ ]

6. Similar varieties and differences between these varieties Denomination of similar variety Characteristic in which the similar variety is different o) State of expression of similar variety State of expression of candidate variety

______________
o)

In the case of identical states of expressions of both varieties, please indicate the size of the difference.

7. Additional information which may help to distinguish the variety 7.1 7.2 Resistance to pests and diseases Special conditions for testing the variety 7.2.1 Day length conditions which favor full bulb development (a) short day (b) long day 7.2.2 Dry matter content (a) low (b) medium (c) high [ ] [ ] [ ] [ ] [ ]

25

7.2.3 Suitability for storage (a) none (b) short term (c) 7.3 Other information long term [ ] [ ] [ ]

8. Authorization for release (a) Does the variety require prior authorization for release under legislation concerning the protection of the environment, human and animal health? Yes [ ] No [ ]

(b) Has such authorization been obtained? Yes [ ] No [ ]

If the answer to that question is yes, please attach a copy of such an authorization.

[end of document]

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