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BITPonlinetest SampleQuestions

The document provides a sample of questions for an online test for the Biotech Industrial Training Programme (BITP) 2013-14. It includes 10 questions testing basic science concepts, 8 questions on basic biotech topics, and 5 more difficult biotech questions. The questions cover a range of topics including enzymes, microbes, DNA, proteins, and industrial biotech applications.

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Ankur Bhatia
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3K views3 pages

BITPonlinetest SampleQuestions

The document provides a sample of questions for an online test for the Biotech Industrial Training Programme (BITP) 2013-14. It includes 10 questions testing basic science concepts, 8 questions on basic biotech topics, and 5 more difficult biotech questions. The questions cover a range of topics including enzymes, microbes, DNA, proteins, and industrial biotech applications.

Uploaded by

Ankur Bhatia
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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Biotech Industrial Training Programme (BITP) 2013-14 Sample Questions for Online Test

Basic Science: 1. The class of enzymes catalyzing transfer of electrons is called: a. Isomerases b. Transferases c. Lyases d. Oxidoreductases 2. Which amongst the following is the strongest electron donor? a. CH3 b. CH3CH2 c. CH3CH2CH2 d. CH3CH2CH2CH2 1 Mark

1 Mark

3. What is the approximate molarity of pure H2O at 25 degrees C and 1 atmosphere pressure? 1 Mark a. 18 b. 55 c. 40 d. 80 4. If x-7=2y and x = 5+3y, what is the value of y? a. -5 b. -2 c. 2 d. 5 1 Mark

5. How many mL of water must be added to 300 mL of 0.75 M HCl to dilute the solution to 0.25 M? 1 Mark a. 900 ml b. 600ml c. 300ml d. 930ml Basic Biotech 1. Which of the following have groups of spherical cells: a. Streptococci b. Staphylococci c. Pediococci d. Pneumococci 2. Enzyme papain is used with success to: a. Leaven bread b. Ripen papaya fruit c. Increase meat production 1 Mark

1 Mark

d. Tenderize meat

3. To optimize the bioreactor system which one of the following conditions is the least important for anaerobic fermentation? 1 Mark a. Control parameter like pH b. Maintenance of temperature c. Respiratory Quotient d. Culture agitation 4. Which one of the following is not true about DNA microarray technology? 1 Mark a. The technology is derived from computer chip manufacture b. Technology works best for organism whose genome is completely sequenced c. The technology is used to access transcription from multiple gene simultaneously d. An electron microscope is used to gather data from the arrays 5. An animal cell line was transfected with DNA extracted from a tumor tissue. Which one of the following will be diagnostic for tumorous transformation? 1 Mark a. Altered cell shape b. Contact inhibition c. Anchorage independent cell division d. Increased duration of cell cycle 6. Which enzyme of the citric acid cycle is inhibited by malonate? a. Citrate synthetase b. Succinyl CoA synthetase c. Pyruvate dehydrogenase d. Isocitrate dehydrogenase 1 Mark

7. A new antibiotic was discovered which strongly inhibited mRNA precursor transcripts and snRNA transcript formation. This antibiotic could be an inhibitor of: 1 Mark a. RNA polymerase I b. RNA polymerase II c. RNA polymerase III d. Helicase 8. Which of the following is isomerized by light as the first event in visual excitation?
1 Mark

a. b. c. d.

Retinol All-trans retinal Retinoic acid 11 cis-retinal 1 Mark

9. Opsonization process relates to a. B cells b. T Cells c. Hepatocytes d. Macrophages

10. The Regulatory agency in India which approves containment facility for use of GMOs involving 20 litre capacity and above. 1 Mark a. Institutional Biosafety Committee

b. Genetic Engineering Appraisal Committee c. Review Committee on Genetic Manipulation. d. National Biotechnology Regulatory Authority

Biotech Difficult 1. A solution of protein has an absorbance at 280nm of 0.64 in a 0.5cm path length cuvette. Given that the molar extension coefficient for the same protein is 4x103 M-1cm-1, the concentration of protein solution is 2 Mark a. 20M b. 0.2 mM c. 1x1010M d. 0.1mM 2. Match the industrial application of the following enzymes: a. Pencillinase 1. Pharmaceutical b. Pectinase 2. Leather c. Protease 3. Wine d. Rennin 4. Dairy a-1 b-3 c-2 d-4 a-1 b-2 c-3 d-4 a-4, b-3 c-1 d-2 a-4 b-2 c-3 d-1 2 Mark

3. Which one of the following partial amino acid sequences is likely to be found in trans membrane protein 2 Mark a. -Leu-Glu-Ala-Ser-Val-Pheb. -Leu-Val-Pro-Asp-Ile-Metc. -Leu-Arg-Val-Leu-Val-Lysd. -Leu-Val-Met-Phe-Ala-Ile4. Ames test is performed to determine a. carcinogenicity of chemicals; b. sporulation of bacteria c. pathogenicity of a bacterium; d. motility of a bacterium 2 Mark

5. A linear ds DNA radiolabelled at only one end (denoted as *) is partially digested with EcoRI (E). The DNA is electrophoresed in a agarose gel. The DNA bands are blotted on to a nitrocellulose and later exposed to X-ray film. The molecular weights of the band were determined. The band/s that would be observed on X ray films is/are 2 Mark

4kb 5 kb 6 kb 2 kb *_______________________________________________ E E E
a. b. c. d.

4, 5, 6 and 2 kb fragments 9, 11, 8 kb fragments 4 kb fragment only 4, 9, 15 and 17 kb fragments

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