Preparative Chromatography

t  he sum of possibilities

LaPrep Sigma the new compact preparative chromatography system from VWR
Being a truly modular system with many possible configurations, having the smallest foot print in the market, an easy to use touch screen panel and ethernet connection, it is the ideal preparative HPLC system for modern purification. Running on the intuitive EZChrom platform, the new LaPrep Sigma has capabilities ranging from analytical to preparative scale, pump capacity from 1 to 600 ml/min as well as easy connection to a fully automated fraction collector. Along with the option of a single or multiple wavelength UV detector and options for autosampler or single injection sample delivery, the new LaPrep Sigma from VWR is an excellent choice for any prep lab whatever the configuration requirements.

LaPrep - The sum of possibilities

From bench use in research laboratories to scale up laboratories: the range of capacity of the pump heads, with maxmimum flow rate for each solvent pump at 50, 200 and 600 ml/min, means that it is highly flexible for use in all chemistry laboratories. The flexible flow rate capacity coupled with the modular design means that it can be a really cost effective solution.

Adapt LaPrep to your application

Versatile, modular system Stand-alone mode for all modules Pump heads with titanium inlays for all sample contact components Double piston pump with electronic residual pulsation suppressor and active piston seal wash Multi-purpose valves, pumps with titanium inlays Optional combination with autosampler or fraction collector Manual or fully automated control Small footprint

| VWR International | Preparative Chromatography

Pumps
Each pump head is manufactured using titanium for all sample contact components. This makes LaPrep Sigma pumps suitable for use with biological samples where a high salt concentration buffer would otherwise corrode stainless steel. All pumps are double piston with electronic residual pulsation suppressor and active piston seal wash which are fitted at the site of manufacture and tested before shipping. The capacity range of the pump heads, with maximum flow rate for each at 50, 200 and 600 ml/ min, means that it is highly flexible for use in all chemistry laboratories from bench use in research laboratories to scale up laboratories. As shown in Table 1, with its range of flow rates, the LaPrep Sigma covers loading capacity of up to 5 g and loading volume of 80 ml. This flexible flow rate capacity coupled with the modular design means it can be a really cost effective solution.

Table 1: Flow rate increments of the VWR LaPrep Sigma

Solvent delivery VWR Hitachi Chromaster HPLC system VWR LaPrep Sigma with 200 ml head Column dimension Typical flow rates LxD (mm) (ml/min) 250 - 4 250 - 10 250 - 25 250 - 50 400 - 100 1 6 39 156 625 Loading capacity (mg) 5 30 200 800 5.12 Loading volume (l) 5 - 80 30 - 500 200 - 3.000 800 - 12.500 5.120 - 80.000 VWR LaPrep Sigma SemiPrep with 50 ml head VWR LaPrep Sigma with 600 ml head Solvent delivery

Preparative Chromatography | VWR International |

Configuration and software

Running on the intuitive EZChrom platform, the new LaPrep Sigma is a compact preparative chromatography system from VWR. Being a truly modular system, with the smallest footprint in the market, an easy to use touch screen panel and ethernet connection LaPre Sigma is an ideal addition to any laboratory needing a flexible prep solution. The LaPrep Sigma has capabilities ranging from analytical to preparative scale, pump capacity from 1 to 600 ml/min, as well as easy connection to a fully automated fraction collector. Along with the option of a single or multiple wavelength UV detector and options for autosampler or single injection sample delivery, the new LaPrep Sigma from VWR is an excellent choice for any prep lab whatever the configuration requirements. As with all VWRs chromatography instrumentation, the new LaPrep Sigma is designed with robustness and durability in mind.

Fraction and system control with EZChrom Elite

The LaPrep Sigma can run in a Semi-prep mode with the 50 ml/min pump. In addition, because it is controlled by EZ Chrom software it can be combined with the VWR Hitachi Chromaster analytical system in order to provide a simple, easy to use semi-prep system whilst taking advantage of the Chromasters excellent autosampler and detector capabilities*. The EZ Chrom software allows for straight forward peak recognition. It starts to search for substance peaks at a pre-determined time. The substance peaks are recognised by looking for criteria which fulfil the peak start and end level. This leads to a simplification of the programming needed for a complete fraction as only one line needs to be programmed for a complete fraction with the same parameters. The peak recognition and subsequent fraction collection can clearly be seen in Figure 1. The EZChrom Elite software using the EZ Prep Driver clearly displays colour coded areas of the chromatogram to represent peak collection, peak recycling or solvent recycling. Figure 1 shows the Signal Level / Slope command in operation for fraction collection.

Figure 1. EZChrom Elite showing the collection of half a peak and a full peak using the Signal Level / Slope command to switch the flow between waste and fraction collection vessel.

* configurations involving a mixture of Chromaster and LaPrep Sigma modules are suitable for research purposes only.

| VWR International | Preparative Chromatography

Direct control of fraction collection

It is also very simple to directly control either the fractionation valve or fraction collection via the direct control tool in EZ Prep. Figure 2 shows the control panel for the fractionation valve. With only a few clicks of the mouse, the flow can be diverted into the corresponding fractionation vessel until a further command diverts the flow to another collection vessel or to waste.

Figure 2. Shows direct control panel with the corresponding collected peaks in the chromatogram.

When trying to separate two or more components that elute at similar times with an overloaded column, peak recycling can be set-up, where by unresolved fractions are fed back into the system in order to undergo further separation. This is particularly suited to processes where only one fraction is of interest and a more environmentally sound separations process is being sought. With preparative chromatographers in todays laboratories being required to provide ever more bespoke and tailored solutions to medicinal chemists, materials chemists and chemical engineers, VWR has designed a highly flexible system to suit the vast majority of cases. VWR can offer a complete package for preparative chromatography, including self-pack and pre-packed columns, solvents, syringes and vials.

Preparative Chromatography | VWR International |

Software, control and start-up

Cat. No.
905-0536 906-0079 905-0535 905-0078 905-0520 905-0519

Type
Ethernet module connector Software Driver Interface box IF2 Start-up kit with 1/16" tubing Start-up kit with 1/8" tubing For connection of LaPrep Sigma modules via ethernet EZChrom Elite versions 3.3.2, SP2 LaPrep Sigma driver for EZChrom Elite versions 3.3.2.SP2 4-channel analogue data input for use with EZChrom Elite, D/A - A/D interface for third party products Contains all fittings and tubing needed for 1/16 set-up Contains all fittings and tubing needed for 1/8 set-up

| VWR International | Preparative Chromatography

Pumps/solvent delivery options

Common to all pumps
Features Double piston pump (main and auxiliary piston) active piston seal washing Titanium inlays for all sample contact components Electronic residual pulsation suppressor Pressure sensor Control LAN, RS232 Touch screen on module front Terminal strip events, terminal strip remote Programming 10 programmes, 9 programme links, WAKE UP programme 50 ml pump head 0.01 - 25 ml/min at 200 bar, 0.01 - 50 ml/min at 150 bar Static dynamic mixing chamber (350 l volume), quaternary low pressure gradient 10 programs, 9 programme links, WAKE UP programme Quaternary low pressure gradient, ranges 0 100%, 1% increments <1% at 0.4 to 40 ml/min <0.4% at 5 ml/min relative standard deviation <0.5% at 1 ml/min 242x352x165 mm plus 45 mm depth for pump head 200 ml pump head 0.1 - 200 ml/min 200 bar 2% at 2 to 50 ml/min <0.1% at 1 ml/min relative standard deviation <0.5% at 1 ml/min 242x352x165 mm plus 45 mm depth for pump head Ternary, 10 - 220 ml/min Stainless steel 600 ml pump head 0.1 - 600 ml/min 100 bar 2% at 2 to 50 ml/min <0.1% at 1 ml/min relative standard deviation <0.5% at 1 ml/min 242x352x165 mm plus 45 mm depth for pump head Binary, 10 - 1000 ml/min Stainless steel 5.9 Ml 1/8 With adapter for 1/16 Stainless steel Up to 250 ml/min 250 Bar 4.8 Ml Stainless steel Up to 250 ml/min 250 Bar

Cat. No.
905-0502

Type
LP1100 (50 ml)

Technical specifications
Characteristics Flow range Extra features for the LP 1100 Programming Gradient Accuracy Precision Residual pulsation WxDxH

Cat. No.
905-0503

Type
LP1200 (200 ml)

Technical specifications
Characteristics Flow range Max. pressure Accuracy Precision Residual pulsation Wxdxh

905-0522

LPG accessory

Low pressure gradient flow rate Material

Cat. No.
905-0504

Type
LP1200 (600 ml)

Technical specifications
Characteristics Flow range Max. pressure Accuracy Precision Residual pulsation WxDxH

905-0521

LPG accessory

Low pressure gradient flow rate Material

Dynamic mixing chambers (advisable for all lpg high flow rate set-ups) 905-0014 Dynamic mixing chamber Volume Tubing Material Flow range Max. pressure 905-0015 Extension for dynamic mixing chamber Volume Material Flow range Max. pressure

Preparative Chromatography | VWR International |

Assistant (sample injection)

For 1/16 capillary set-up Cat. No. Type
manual injection 905-0512 Manual valve Tubing Features Includes Automated injection: LaPrep Sigma Assistant 905-0514 Semipreparative assistant Tubing Feed pump Injection valve Fractionation valve Wxdxh Automated injection: Feed pump + injection valve 905-0500 LP1000 Characteristics Flow range Max. pressure 905-0501 LP1000 Characteristics Flow range Max. pressure Features 10 ml pump head 0.001 9.999 ml/min 400 bar 50 ml pump head 0.01 49.99 ml/min 150 bar Double piston pump (main and auxiliary piston) active piston seal washing Titanium inlays for all sample contact components Electronic residual pulsation suppressor Pressure sensor Control LAN, RS232 Keys on module front (2 function keys, Start/Stop key) Terminal strip remote Analog out Accuracy Precision WxDxH 905-0507 LP2002, 1/16 Features Tubing WxDxH Sample loops for automated injection 905-0029 905-0030 Tubing Volume Tubing Volume 1/16 10 ml 1/16 40 ml 1% at 1 ml/min <0.5% at 1 ml/min relative standard deviation 242x352x138 mm Valve with drive, 6 ports, 3 channels, 2 positions 1/16 122x188x140 mm Lp1000 Lp2002 Lp2016 1/16 10 ml pump head, 400 bar for details see 905-0500 6 port, 3 channels, 2 positions for details see 905-0507 17 ports, 1 channel, 17 positions for details see 905-0511 242x352x137 mm plus pump head and valve 1/16 6 port, 3 channels, 2 positions Signal cable, mounting bracket

Technical specifications

Design note:
The assistant is key to the flexibility of LaPrep Sigma. The assistant can house the fractionation valve, injection valves and automated injection pump, meaning that minimal bench space is used and a variety of configuration options are available.

| VWR International | Preparative Chromatography

Assistant (sample injection) cont.

For 1/8 capillary set-up Cat. No. Type
905-0515 Semipreparative Assistant

Technical specifications
Tubing Feed pump Injection valve Fractionation valve WxDxH LP2002 1/8 Feed pump LP1000, 50 ml pump head, 150 bar details see 905-0501 6 ports, 3 channels, 2 positions for details see 905-0508 13 ports, 1 channel, 17 positions for details see 905-0510 242x352x137 mm plus pump head and valve 150 bar 50 ml pump head 0.01 49.99 ml/min 150 bar Double piston pump (main and auxiliary piston) active piston seal washing Titanium inlays for all sample contact components Electronic residual pulsation suppressor Pressure sensor Control LAN RS232 Keys on module front (2 function keys, Start/Stop key) Terminal strip remote Analog out Accuracy Precision WxDxH 1% at 1 ml/min <0.5% at 1 ml/min relative standard deviation 242x352x138 mm Valve with drive, 6 ports, 3 channels, 2 positions 1/8 122x188x140 mm 1/8 10 ml 1/8 40 ml

automated injection: LaPrep Sigma Assistant

Automated injection: feed pump + injection valve 905-0501 LP1000 Max. pressure Characteristics Flow range Max. pressure Features

905-0508

LP2002, 1/8

Features Tubing WxDxH

Sample loops for automated injection 905-0031 905-0032 Tubing Volume Tubing Volume

Alias autosampler
Cat. No.
905-0124

Technical specifications
Vial dimensions (cap included) Dispenser syringe Injection volume Valve Sample loop Buffer tubing Needle Max. vial height: 47 mm, Min. vial height: 32 mm 2500 BL syringe 1 l - 10.000 l, with 1 l increments Valco 0.75 bore valve 10 ml SS sample loop, 1/8 tubing with 1/16 tubing ends and fittings (Valco) 2 ml LVS needle

Preparative Chromatography | VWR International |

Detector options
Common features
Type Light source Wavelength Integration time Control Fast scanning two-beam monochromator spectrophotometer Deuterium lamp 190 - 750 nm 500 ms 12 ms, determined automatically LAN, RS232 Touch screen on module front Terminal strip: Event and remote control Programming WxDxH Time controlled: Wavelengths, events, valve control, programme links, 19 programmes, WAKE UP programme 242x352x169 mm plus flow cell

Cat. No.
905-0505

Type
LP3101

Technical specifications
1-channel UV detector Type Light source Wavelength Accuracy Precision Optical bandwidth Noise Drift Linearity Fast scanning two-beam monochromator spectrophotometer Deuterium lamp 190 - 750 nm 2 nm 0.7 nm residual standard deviation 8 nm 0.75 x 10 - 5 AU/h at 254 nm 1.5 x 10 - 4 AU/h at 254 nm >2.5 AU Fast scanning two-beam monochromator spectrophotometer Deuterium lamp 190 - 750 nm 1 nm 0.4 nm residual standard deviation <5 nm 0.5 x 10 - 5 AU/h at 254 nm 0.5 x 10 - 4 AU/h at 254 nm >3 AU 1/8 0.5/1.25/2 mm selectable path length 25 l 1000 ml/min 200 bar 1/16" 0.5 mm 3 l 250 ml/min 200 bar 1/16 3 mm 2 l 50 ml/min 300 bar

905-0506

LP3104

4-channel UV detector Type Light source Wavelength Accuracy Precision Optical bandwidth Noise Drift Linearity Flow cell option

905-0530

Preperative flow cell

Tubing Path length Volume Max. flow rate Max. pressure

905-0531

Semi-preparative flow cell

Tubing Path length Volume Max. flow rate Max. pressure

905-0529

Analytical flow cell

Tubing Path length Volume Max. flow rate Max. pressure

10

| VWR International | Preparative Chromatography

Fractionation
1/16" fractionation Cat. No. Type
Fractionation valve 905-0511 LP2016 Tubing Features WxDxH Fractionation using the assistant 905-0514 Semi-preparative assistant Tubing Feed pump Injection valve Fractionation valve WxDxH 905-0517 Assistant Tubing Functionality WxDxH LP1000 LP2002 LP2016 1/16 10 ml pump head, 400 bar for details see 905-0500 6 ports, 3 channels, 2 positions for details see 905-0507 17 ports, 1 channel, 17 positions for details see 905-0511 242x352x137 mm plus pump head and valve 1/16 2 valves, 7 ports, 1 channel, 7 postions 242x352x137 mm plus pump head and valve 1/16 Fractionation valve with drive, 17 ports, 1 channel, 17 positions 122x188x140 mm

Technical specifications

1/8" fractionation Cat. No. Type

Fractionation valve 905-0509 LP2006

Technical specifications
Tubing Features WxDxH 1/8 Valve with drive, 7 ports, 1 channel, 7 positions 122x188x140 mm 1/8 Valve with drive, 13 ports, 1 channel, 13 positions 122x188x140 mm 1/8 Feed pump LP1000, 50 ml pump head, 150 bar for details see 905-0501 LP2002 LP2012 6 ports, 3 channels, 2 positions for details see 905-0508 13 ports, 1 channel, 17 positions for details see 905-0510 242x352x137 mm plus pump head and valve 1/8 2 valves LP2006, 7 ports, 1 channel, 7 positions 242x352x137 mm plus pump head and valve

905-0510

LP2012

Tubing Features WxDxH

Fractionation using the assistant 905-0515 Semi-preparative assistant Tubing Functionality Injection valve Fractionation valve WxDxH 905-0516 Assistant Tubing Functionality WxDxH

Fractionation collectors Cat. No. Type

905-0090 905-0125 905-0533 Vario 2000 Vario 4000 Foxy R1 Stand-alone package Foxy R2 stand-alone package

Technical specifications
Flow rate Flow rate Flow rate Capillaries Tube Flow rate Capillaries Tube <20 l/min <100 l/min <25 ml/min 1/16 18 mm ext. <125 ml/min 1/8 18 Mm ext.

905-0534

* further accessory options are available

Preparative Chromatography | VWR International |

11

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