name: number :

_____________ ______________

BCH 2333A, BCH 2333B & BCH 2333C FINAL EXAM April 21, 2008 Professors: Length : 3 hours Material needed: CLOSED BOOK EXAM. NO MODELS ALLOWED Faculty approved calculators are permitted This exam, 1 computer answer sheet Bennett, Kleine, Mezl

INSTRUCTIONS Part I Chose the best answer for each of the 60 MCQ questions. (60 % of grade) Answer FOUR of the 6 essay questions directly on the exam. YOU MUST ANSWER ONE QUESTION FOR EACH PROFESSOR Extra questions will not be corrected. (40% of grade)

Part II

AT THE END: - Check that your name and student number are on the exam and on the computer answer sheet.

1.

Penicillin inactivates the enzymes that A) B) C) D) E) replicate DNA translate mRNAs cross link collagens cross link the peptidoglycans of bacterial cell walls cross link the lipid bilayer of the bacterial membrane

2.

With a 3D visualization program like CHIME, to see how an atom is protected by a protein structure (like Fe in hemoglobin) you would ask for the display: A) Wireframe D) Ball & Stick B) Strand E) x,y,z coordinates C) Spacefill

3. A) B) C) D) E) 4.

Coulombs law gives : the repulsion between two molecules as a function of their charge and the dielectric constant the repulsion between two molecules as a function of their mass and their dipole moment the attraction between two molecules as a function of their charge and the dielectric constant the attraction between two molecules as a function of their mass and the dielectric constant the attraction between two molecules as a function of their mass and their dipole moment The length of a C-H bond is about : A) 0.015 nm B) 0.11 nm C) 0.17 nm D) 0.28 nm E) 1.5 nm

5.

The pH of 1 mL of 20 mM acetate buffer is 4. The pK of acetic acid is 4. When 5 microlitres of 1 N HCl are added to this solution, the pH will be about : A) 3.9 B) 3.5 C) 3 D) 2.7 E) 2

6.

A weak acid has a Ka of 2 10 -5. In water a solution of this acid will have a pH of about : A) 3.2 B) 3.7 C) 4.7 D) 5.3 E) This can not be determined from this data

7.

In pure water, about ......... water molecules are ionized. A) 0.01% of the B) one in 106 C) one in 109 D) one in 1012 E) one in 1014

8.

Estimate the total amount of hydrogen ions present in all the blood in your body: A) about 0.5 millimoles D) about 50 nM B) about 0.5 micromoles E) about 5 10-7 M C) about 0.5 nanomoles

9.

A chromosome contains 5 105 nucleosomes. This chromosome is about ...... base pairs long. A) 2 105 B) 1 106 C) 5 106 D) 20 106 E) 100 106

10.

The atom at position 5 of uracil is joined to A) B) C) D) E) a nitrogen, a carbon and a hydrogen a nitrogen, a carbon and two hydrogens a nitrogen, an oxygen and a hydrogen two carbons and a hydrogen two carbons and two hydrogens

11.

The reaction that makes a bond between two nucleotides : A) an oxidation D) a dehydratation B) a reduction E) a tautomerisation C) a hydrolysis

12.

A nucleotide inside a DNA molecule has a free alcohol group : A) at the 5' position D) at the 2' and 3' position B) at the 2' position C) at the 3' position E) it does not have a free alcohol group

13.

In DNA for the sugar, the ratio of -anomer to -anomer is about: A) 1 : 0 B) 3 :1 C) 1 :1 D) 1:3 E) 0:1

14.

If you lined up all the DNA of a 2 105 base pair chromosome as a Watson-Crick helix, the length would be about A) 1.5 cm B) 3 mm C) 0.6 mm D) 1.5 10-6 m E) 0.03 10-6 m

15.

Which of the following statements about tRNA in eukaryotes is FALSE? A) B) C) D) E) If it is heated one observes a phenomenon called hyperchromicity It is about 80% of the total cellular RNA The bases are joined together by 3'-5' phosphodiester bonds tRNA will be hydrolysed if it is in a basic solution tRNAs all have double stranded regions and single stranded regions

16.

..... makes DNA shine orange in UV light. A) D) Cupric ion Iodine B) Ethidinium bromide E) Periodate C) Ninhydrin

17.

Thymine has a chiral atom at position : B) 2 et 4 C) 5 D) 6 E) Thymine does not contain a chiral atom

A) 1', 2', 3', 4' 18.

When one considers the diameter of possible DNA structures, going from the thinnest DNA to the thickest the order is: A) A, B, Z B) A, Z, B C) B, A, Z D) Z, B, A E) Z, A, B

19.

Which is the biggest molecule among the following? A) cytosine B) guanosine C) adenine D) thymine E) uracil

20.

This element gives a negative charge to some mucopolysacharides : A) Ca B) Cl C) I D) N E) S

21.

Use this structure, a sugar called testose, to answer the following questions: The sugar on the left side of testose is : A) B) C) D) E) D-glucose D-fructose L-mannose an L-sugar but not one of the ones to know by heart a D-sugar but not one of the ones to know by heart

22.

The sugar on the left of testose has a prochiral atom at position : A) 1 B) 6 C) 1 and 6 D) 1, 2 and 6 E) It does not have a prochiral atom

23.

The sugar on the left of testose has a deoxy group at position: A) 1 B) 6 C) 1 and 6 D) 1, 2 and 6 E) It does not have a deoxy group

24.

The sugar on the right of testose is a : A) D-aldose B) D-ketose C) L-aldose D) L-ketose E) D-lactone

25.

The sugar on the right of testose is a/an : A) hemi-acetal B) acetal C) hemiketal D) ketal E) lactone

26.

The sugar on the right of testose is : A) B) C) D) E) D-glucose D-fructose L-mannose an L-sugar but not one of the ones to know by heart a D-sugar but not one of the ones to know by heart

27.

The bond between the two sugars is a ............ bond. A) 1, 1 B) 1,6 C) 1, 6 D) 6, 6 E) 6,6

28.

The sugar on the right side is the ..... isomer and the sugar on the left side is the .... isomer. A) , B) , C) , D) , E) lactone,

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58

..........D... ......C....... A............. ...B.......... ...B.......... .............E ......C....... ...B.......... .............E ..........D... ..........D... .............E A............. ......C....... ...B.......... ...B.......... .............E ..........D... ...B.......... .............E ...B.......... ......C....... .............E A............. A............. A............. ...B.......... A............. ...B.......... ......C....... ..........D... .............E ......C....... ...B.......... .............E ......C....... A............. .............E ......C....... ..........D... ......C....... ...B.......... A............. ..........D... A............. ...B.......... ...B.......... A............ .............E .............E A............. ......C....... ...B.......... ..........D... xxxxxxxxxxxxxxxx ......C....... .............E ......C.......

59 A............. 60 ..........D... <bch233340823 <48 e to A 38 b to e bch2733> >_