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    Lab SalivaryAmylase PDF

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    Sip Bio
    The document describes a lab experiment to observe the action of the enzyme amylase in saliva. Students will test samples of starch solution, saliva, and a mixture of saliva and starch using Lugol's iodine to detect starch and Benedict's solution to detect simple sugars. This will allow them to determine if amylase in saliva is breaking down starch into glucose. The experiment tests the hypothesis that saliva contains enzymes that break down starch into simple sugars.

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    Lab SalivaryAmylase PDF

    Uploaded by

    Sip Bio
    30K views3 pages
    The document describes a lab experiment to observe the action of the enzyme amylase in saliva. Students will test samples of starch solution, saliva, and a mixture of saliva and starch using Lugol's iodine to detect starch and Benedict's solution to detect simple sugars. This will allow them to determine if amylase in saliva is breaking down starch into glucose. The experiment tests the hypothesis that saliva contains enzymes that break down starch into simple sugars.

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    The document describes a lab experiment to observe the action of the enzyme amylase in saliva. Students will test samples of starch solution, saliva, and a mixture of saliva and starch using Lugol's iodine to detect starch and Benedict's solution to detect simple sugars. This will allow them to determine if amylase in saliva is breaking down starch into glucose. The experiment tests the hypothesis that saliva contains enzymes that break down starch into simple sugars.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    30K views3 pages

    Lab SalivaryAmylase PDF

    Uploaded by

    Sip Bio
    The document describes a lab experiment to observe the action of the enzyme amylase in saliva. Students will test samples of starch solution, saliva, and a mixture of saliva and starch using Lugol's iodine to detect starch and Benedict's solution to detect simple sugars. This will allow them to determine if amylase in saliva is breaking down starch into glucose. The experiment tests the hypothesis that saliva contains enzymes that break down starch into simple sugars.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 3

    Mr. Ulrich/Mrs.

    Ryan Regents Biology

    Salivary Amylase Lab


    Background: Enzymes are protein catalysts. This means they are chains of amino acids with a particular shape that allows them to interact with a specific molecule called a substrate to bring about a chemical reaction. When the reaction is complete, the enzyme is unchanged and able to interact with another substrate molecule. Many enzymes are present in your digestive system. Each digestive enzyme breaks down a specific type of macromolecule. For example, pepsin breaks down protein and lactase breaks down lactose, a disaccharide. Digestion begins in your mouth with saliva which contains an enzyme called amylase. Amylase works to break down complex carbohydrates. Through hydrolysis, amylase breaks down the polysaccharide, AKA starch, into the monosaccharide, glucose. In this experiment, Lugols Iodine solution will be used to test for the presence of polysaccharides in test tubes and Benedicts solution will be used to test for the presence of simple sugars. Start salivating.youre going to need it!
    (Think foodie thoughts)

    Purpose: To observe the results of enzyme action. Pre-lab questions: What does Lugols iodine solution test for? What is the color change for a positive Lugols test? What does Benedicts solution test for? What is the color change for a positive Benedicts test? What do we call compounds that change color to show chemical properties? What determines a proteins shape?

    Material: 6 test tubes two labeled A, two B and two C Test tube rack Test tube holder Hot plate with beaker of boiling water Starch solution Corn starch Lugols iodine solution with dedicated pipet Benedicts solution with dedicated pipet Safety goggles 10-ml graduated cylinder

    Procedure: First we need to prepare our solutions for testing. There will be two of each preparation one set for the Benedicts/simple sugar test and one set for the Lugols/starch test. 1. Using a graduated cylinder measure 5 ml of starch solution into each A test tube and put them in the test tube rack. 2. Work up a good amount of spit in your mouth and spit it into the B test tubes. You dont need to measure it but it should fill about 1 cm of the tube. Put them in the rack. 3. Now for the fun part. Put about tsp (the tip of a plastic spoon) of dry cornstarch in your mouth. DO NOT SWALLOW IT. Its not poisonous. We need it for testing though. 4. Slosh it around in your mouth for a moment until it is a runny goo and then spit it into test tube C. If it sticks to the side of the tube and doesnt run the side into the bottom of the test tube it wasnt runny enough. 5. Repeat the process for the other test tube C. Now we set up the sugar test. 1. Take one set of test tubes (A, B, and C) and put 5 drops of Benedicts solution into each test tube. 2. Swirl each tube and carefully place them in the boiling water bath. 3. Wait 5 minutes for results. While waiting, go on to the starch test. While the sugar test runs, complete the starch test. 1. Put 5 drops of Lugols solution into each of the remaining test tubes (A, B, and C) in the rack. 2. Note the color change (if any) of each test tube in table 1. 3. Analyze the results (what do the results tell us about the presence of starch in each tube?) in table 1 as well. 4. After 5 minutes, note color changes and analyze the results from the sugar test (is sugar present?) in table 1. Results: Test tube A (starch soln) B (saliva) C (Saliva and starch) Iodine test (include color) Starch present? Benedicts test (include color) Simple sugars present?

    Analysis and Conclusion Questions: 1. What would be a good hypothesis for this experiment?

    2. Prove or disprove this statement: Saliva contains enzymes that break down starch into simple sugars.

    3. Why did we include water and starch test tube in the experiment?

    4. Why did we include the test tube with only saliva in the experiment?

    5. Mr. Ulrich loves cheese but it doesnt start chemically breaking down in the mouth. Why not?

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