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Title: Experiment 1 Techniques of Using Microscope Student'S Name: Angie Lawrence Student'S Id: CPM13/12D

The document describes an experiment using a light microscope to view human blood cells. It explains the basic parts and use of the microscope, including adjusting the coarse and fine knobs to bring the sample into focus at different magnifications levels. Under low and high power objectives, human blood cells were visible at 400x and 1000x magnification. Proper techniques like starting with lower magnification and protecting the lenses were discussed.

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Angie Lawrence
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70 views6 pages

Title: Experiment 1 Techniques of Using Microscope Student'S Name: Angie Lawrence Student'S Id: CPM13/12D

The document describes an experiment using a light microscope to view human blood cells. It explains the basic parts and use of the microscope, including adjusting the coarse and fine knobs to bring the sample into focus at different magnifications levels. Under low and high power objectives, human blood cells were visible at 400x and 1000x magnification. Proper techniques like starting with lower magnification and protecting the lenses were discussed.

Uploaded by

Angie Lawrence
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOCX, PDF, TXT or read online on Scribd
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TITLE

: EXPERIMENT 1 TECHNIQUES OF USING MICROSCOPE

STUDENTS NAME STUDENTS ID PROGRAMME LECTURER

: ANGIE LAWRENCE : CPM13/12D : FOUNDATION IN MEDICAL STUDIES : MRS. TANU PRAMANIK

Introduction Cells are structural and functional units of life. They are microscopic units that can only be observed under a microscope. There are two types of microscopes used to study cells: light microscope and electron microscope. Light microscope uses light as the source of radiation and electron microscope uses electrons as the source of electron. Compound lenses and light are used to magnify objects in light microscope, visible lights are passed through the specimens, and the glass lenses will then bend the light, which enlarges the image of the objects beneath making them to appear closer. It is then projected into human eyes. Another type of light microscope is the stereoscope which allows a binocular viewing of larger specimens. For electron microscopes, the resolving power is much better than the light microscope and there are more than 1000 fold improvement compared to the light microscope. There are two types of electron microscope, which are the transmission electron microscope and scanning electron microscope. Transmission electron microscope is used to study the internal structures of the cells. Beam of electrons will be passed through the specimens before being viewed. The electrons will be directed and only the transmitted electron can be seen. The electrons will be focused into an image are then magnified, thus resulting in the magnification of the internal structures of the cell. For the scanning electron microscope, the beam of electrons will be used to scan the surfaces of structures of the cells. Only reflected beam can be seen. The scanning electron microscope is used to scan the surfaces of structures of the cells. The specimens for light microscope can be prepared fresh or living cell but the specimens for scanning electron microscope (SEM) and transmission electron microscope (TEM) must be killed and preserved before being observed.

Experiment 1 Title Objective a) To identify the microscope parts. b) To understand the application of microscope. c) To understand the procedure and precautions taken when using a microscope. Apparatus Light microscope Materials Readily-prepared slides Procedure 1) The light microscopes plug is plugged into the extension cord and the light source is switched on. 2) The objective lens is switched on to start with scanning objective. 3) The human blood cell that is readily prepared on the slide is placed on the stage and is clamped by the stage clips. 4) To get an image, the coarse knob is adjusted. Then, the fine knob is adjusted to get a clearer image. 5) The results and image seen is recorded. 6) Step one to step five are repeated with low power objective and high power objective respectively. : Techniques of using microscope.

Figure 1: Image of light microscope Results/Observations Viewing of human blood cell under a light microscope

Scanning objective (10 x 10 x) 100 x

Low power objectives (40 x 10x) 400 x

High power objective (100 x 10 x) 1000 x

Figure 1: Image of light microscope Results/Observations Viewing of human blood cell under a light microscope

Scanning objective (10 x 10 x) 100 x

Low power objectives (40 x 10x) 400 x

High power objective (100 x 10 x) 1000 x

Discussion 1. When using a microscope, always start with the scanning objective. We need to use the lower power objective to find the image before using a higher power objective because the image cannot be seen if we start with the high power objective. 2. Do not remove the slide when the high power objective is in place as the slide might scratch the lens. 3. The microscope should always be wrapped and covered by the electric cords before returning if to the cabinet. 4. The microscope should always be carried by the arm and set flat on the table. Conclusion Microscope magnifies objects that cannot be seen with our naked eyes.

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