BIOFLO 3000

Universal Fermentor

Versatile System for Fermentation & Cell Culture Research

NEW BRUNSWICK SCIENTIFIC

Where Quality and Innovation Have Become Tradition

The Universal Benchtop Fermentor/Bioreactor for Fermentation and Cell Culture

FILTERED GAS/AIR LINE DETACHABLE DRIVE MOTOR

ALPHA-NUMERIC DISPLAY SCREEN GAS/AIR REGULATOR 2 or 4 GAS CONTROLLER

ioFlo 3000 is an advanced bioprocessing system designed to maximize yields in both fermentation and cell culture. Ideally suited to meet the changing needs of the research laboratory, this versatile fermentor/bioreactor is capable of producing high yields of biomass or secreted products from virtually any microbial, animal, insect and plant cell line. The stirred-tank system comes complete with everything needed for out-of-the-box fermentor start-up, and is offered with a variety of options for dedicated cell culture applications. UNIQUE ADVANTAGES

EXHAUST GAS CONDENSER

STERILE SAMPLER

DATA COMMAND CENTER

DEDICATED CELL CULTURE VESSEL (Optional)

PACKED BED BASKET IMPELLER

FIVE PROGRAMMABLE, ASSIGNABLE PUMPS

Built-in Gas-Mixer Optimizes

Dissolved Oxygen Levels For High-Density Culture. Two-Gas Mixing in Fermentation provides automatic or manual blending of supplemental oxygen and air. Create air, oxygen, and agitation control cascades that respond to oxygen-demanding microbes, while limiting foam and shear. Four-Gas Mixing in Cell Culture, blends air, oxygen, carbon dioxide, and nitrogen for precise and gentle automatic control of both pH and dissolved oxygen
350 mM/L/Hr Oxygen Transfer

WATER IN/OUT

FERMENTOR VESSEL WITH STAINLESSSTEEL JACKET

BioFlo 3000 is available with twelve interchangeable vessel assemblies. Shown above with 6.6 L fermentor vessel and 2.2 L cell culture vessel with packed-bed basket. Sizes refer to total volume.

Choice of Thermal Mass Flow

Meter or Thermal Mass Flow Controller for accurate air flow monitoring and control
Choice of P-I or Deadband

BioFlo 3000 data screen displays setpoints and process variables simultaneously. The vacuum fluorescent alpha-numeric display provides easy access to every phase of the process, including 13 control loops that simplify probe calibration, cell growth and process manipulation.

pH Control. PI control provides maximum accuracy; Deadband minimizes addition of acid/base

Tunable P-I Values Optimize

Rate obtained through powerful fermentation agitation motors and 6-bladed Rushton impellers
Choice of 12 Interchangeable

Vessels. Fermentation vessels offered with or without side ports, in four sizes from 1.6 through 14 liters. Glass-jacketed cell culture vessels, 2.2 through 14 liters total volume
Stainless-Steel Heat Exchanger

ture uniformity and the most efficient heat-transfer characteristics. The best choice for fermentation, especially for high-density cultures and for temperature induction
Vessel Side Ports support

Free Installation and Start-Up

DO Control
Five Assignable, Peristaltic

Assistance Provided
NBS Laboratory Training or

Extensive On-Site Training Packages also available

BioCommand Software

Pumps built in; with control included for optional sixth pump
Stainless-Steel Exhaust

chemostat operation
Low Side Entry Ports (optional)

forms the rounded bottom of fermentation vessel assemblies. Allows the shortest autoclave time, with no impeding doubleinsulating glass to slow sterilization. Large-area high-conductivity steel surface provides superior tempera-

two 19 mm ports in the stainlesssteel heat-exchanger support additional probes, which enter the vessel below the impeller. 12 mm adapter available
Patented Fibra-Cel Packed

(optional) automates data logging, centralized control and programmed process protocols with a powerful and easy-to-use program

Condenser minimizes media evaporation

Stainless-Steel Ring Sparger

provides uniform distribution of bubbles to impeller blades

Battery Back-Up retains

OTHER ADVANTAGES
Integrated Vessel, Console,

Bed, and Cell-Lift Microcarrier Technologies maximize yields of animal cell products* (optional) 2

Controller and Pumps conserve bench space and simplify connections

settings in the event of a power failure

() Available in fermentor vessels only. (*) See CelliGen Plus literature for details

Everything is Included, From Probes to Controllers ...Even System Set-up and Start-up Assistance
NBS supplies virtually every component and accessory needed to execute and control the most complex fermentation processes. All of the accessories shown, and more, are supplied with the fermentation system as standard equipment. In addition, NBS provides the services of a technical professional to assist in equipment set-up and system start-up.

SixBlade Rushton Impeller Assembly

Harvest Tube

Ring Sparger

RTD Sensor & Cable

pH Probe, Cable & Electrolyte DO Probe, Cable & Electrolyte

Aseptic Sampler with Collection Vial

Medium Break Tube for Continuous Culture Vessel Assembly with Detachable Motor and Side Ports

Air O2 CO2 N2 4-Gas Connections

Foam/Level Probe & Cable

Stainless Steel ExhaustGas Condenser Removable with Filters Baffle Assembly

EIA-422/RS-232C Computer Port & Output for 4-Channel Recorder

5 Programmable, Assignable Pumps 4 QuickConnect Water Lines

Advanced Microprocessor (MCS) Control System for Integrated Regulation of 13 Control Loops

Tool Kit with O-Rings and Rubber Diaphragms

Filtered Air Inlet Lines (2)

Pressure Hose and Clamps

OPERATING MODES AND ACCESSORIES

Batch Culture Fed-Batch Culture Continuous Culture Optional dedicated cell culture vessel with Cell-Lift impeller. Optional decanting column or spin filter for perfusion Optional low-shear, pitched blade and marine blade impellers. Optional dedicated cell culture vessels. Optional spin filter for perfusion Optional dedicated cell culture vessel with packed-bed basket impeller for secreted products Includes all required instrumentation as standard

PROCESS CONTROL SYSTEMS SUPPLIED

PARAMETER Temperature Agitation pH DO Foam/Level CONTROL PID PID PID Acid & Base Pump
and 4-gas PID Cascade to Agitation, Gas/Air Flow, & 2 or 4-Gas Electronic Pump for AntiFoam, Nutrient or Harvest

SENSOR SUPPLIED RTD Photo-Optical Disk Ingold or Broadley James Ingold or Broadley James Conductivity Probe Thermal Mass Flow Meter (TMM) or Thermal Mass Flow Controller (TMC)

Microcarrier Cell Culture

Suspension Cell Culture

Packed-Bed Immobilized Cell Culture

Gas/Air Flow

Electronic Monitoring (TMM) or Electronic Control (TMC)

High-Tech Fermentor Vessel Simplifies Operation

FERMENTOR PENETRATIONS: HEADPLATE

Thermowell for platinum RTD Sensor* Ring Sparger Harvest Tube Sampler Assembly DO Electrode* pH Electrode* Acid Addition Base Addition Inoculation (Septum) Exhaust Gas Condenser Foam Level Detector* Anti-Foam Addition Spare Ports
ADJUSTABLE HARVEST TUBE FOUR BAFFLES GLASS TUBE REACTOR FOUR UNBREAKABLE PROCESS PORTS HARVEST (OVERFLOW) PORT

SIDE WALL

Four Process Ports Without Ports (optional)

TEMPERED WATER IN/OUT

HEAT EXCHANGER

2-Side-Entry Probe Ports (optional)

RING SPARGER

(*) Sensors are supplied as standard

SIDE-ENTRY PROBE PORT (OPTIONAL)

Vessel Description
BioFlo 3000 is equipped with a thick-walled glass tube fermentor vessel that provides superior resistance to rough handling and repeated autoclaving. This configuration allows for significant reduction in autoclaving time compared to glass-jacketed vessels. For precise temperature control the stainless-steel base has a dished bottom that is jacketed for the circulation of warm or cool water. The BioFlo 3000 provides a closed-loop temperature control system without the need for external water baths or circulators. The vessels are interchangeable and have working volumes of 1.25, 2.5, 5.0 and 10 liters. All seals are EPDM, silicone or Teflon. Easy handling, set-up, and installation are achieved by a detachable motor that readily couples to the agitator without tools, convenient quick-connects for jacket water circulation, and alignment pins that secure the vessel to the console without tools. In addition to eight fermentor vessels, four cell culture vessel assemblies can also be supplied

QUICK-CONNECT PLUG-IN FITTINGS

HEMISPHERICAL WATER-JACKETED HEAT EXCHANGER (STAINLESS STEEL)

with a choice of five low-shear impellers and a packed-bed basket accessory for immobilized cell culture. For details, please see our CelliGen Plus literature.

The fermentor vessel assembly shown at right is locked into the console without tools or hardware. Plug-in water connections to the jacket and condenser have O-ring seals and snap-locks with instant spring release. The side ports allow for a variety of process configurations such as a chemostat.

Greater Versatility in DO Control Than Ever Before

Dissolved Oxygen Control Strategies
Two operating modes accommodate fermentation and cell culture applications. In both modes the BioFlo 3000 controller features adjustable P-I values for those who want to customize DO response. Fermentation Mode: More than a dozen built-in control cascades let you establish precise control of dissolved oxygen in the way best suited to your application. The Thermal Mass Flow Controller (TMC) equipped BioFlo 3000 can utilize agitation, oxygen enrichment, and air flow, together or in sequence, individually or in combination, to automatically maintain dissolved oxygen at the most productive levels. User-set agitation and gas flow limits let you avoid foaming, and ensure a uniform suspension under all conditions. The BioFlo 3000 has a guaranteed Oxygen Transfer Rate (OTR) of at least 350mM/L/hr at standard pressure using standard air, before oxygen supplementation. Cell Culture Mode: DO and pH control are linked through the built-in 4-gas control system. Precisely metered air, oxygen, C02, and nitrogen are automatically blended to maintain DO and pH at desired levels. With the optional BioCommand software, liquid base additions can be used in tandem with the 4-gas system.

Dissolved Oxygen Control Options in Fermentation Mode

Agitation first

Agitation only

02 Enrichment only

Then 02 Enrichment

Agitation and 02 Enrichment

Gas Flow Rate Control (Liters/minute)

enabled with Thermal Mass Flow Control-equipped fermentors only.

In Fermentors Equipped with a Thermal Mass Flow Meter (TMM): DO concentrations may be readily optimized through control of agitation and/or O2 enrichment. In Fermentors Equipped with a Thermal Mass Flow Controller (TMC): DO control options may be extended by cascading any of these modes either sequentially or simultaneously with air flow,

providing eight additional control options. Air flow alone may also be selected, for a total of 13 built-in control options. With BioCommand Software: Further optimization may be achieved with use of optional BioCommand software to control additional operating parameters such as feeding schedule, temperature or pH.

THE NEW FERMENTATION TECHNOLOGIES

Microprocessor Control System (MCS) An on-board control system has an extensive memory that helps simplify the entire operation, from probe calibration to cell growth and product recovery. The data control center displays over 75 setpoints and process variables in 13 control loops to help optimize fermentation parameters. The alpha-numeric display provides easy access to every phase of the operation, simplifying start-up, cell growth and product manipulation. High Oxygen Transfer Rate Guaranteed A permanent magnet motor is built for high-speed agitation of microbial fermentation as well as gentle stirring of shearsensitive cultures. The drive motor has high torque output and maintains speeds from 20 to 1,000 rpm via high-resolution optical sensor in a feedback control loop. The sensor can lock onto any setpoint with an accuracy of + 1 rpm. Vessel geometry combines with agitator power to achieve a high oxygen transfer rate of 350 mM O2/L/Hr. Electronic Air Flow Control Both a Thermal Mass Flow Meter (TMM) and Thermal Mass Flow Controller (TMC) are offered. The TMM digitally displays air flow data on the fermentors display panel, as well as exports the data to optional software for monitoring and archiving. The TMC not only offers display and export capability, but adds an electronically operated valve that allows the user to cascade air flow with other parameters, either via the built-in microprocessor or optional software. When utilizing optional BioCommand software, air flow can be associated with a time or event profile. Built-In DO Control Strategies Control strategies may be employed to optimize DO using agitation and/or pure oxygen to meet the desired DO concentration. If optimum DO is not reached in the first mode, the operator can switch to the second, third or fourth strategy until the DO setpoint is reached. (See chart above.) Pumping Versatility Five built-in, programmable pumps can be assigned to more than seven different functions via the keypad. These include addition of acid, base, chemical defoamer and nutrients as well as the harvesting of culture, and pump actuation at three different liquid levels. Control capability is also provided for an optional sixth pump.

PC-Compatibility and Bioprocessing Software

BioFlo 3000s broad operating capabilities are extended even further by its PC compatibility and its optional advanced bioprocessing software. The computer facilitates yield optimization strategies as well as programmed data acquisition, data logging and real-time graphics.

Continuous Culture
In addition to routine batch and fed-batch fermentation procedures, the BioFlo 3000 is designed for operation as a chemostat. Nutrient is transferred from an accessory media reservoir into the fermentor via one of the panel-mounted pumps. Pump flow is calibrated to maintain the desired dilution rate, and is indicated on the data display screen. A vessel overflow port is connected to an accessory product reservoir in order to maintain steady-state growth conditions in the fermentor.

BioFlo 3000 Fermentor Specifications

VESSEL VOLUME VESSEL DIMENSIONS for Autoclave2 VESSEL DIMENSIONS with Slant Rack Total Capacity (L) Working Maximum (L) Working Minimum (L) Height Diameter Height Diameter 19 9.5 11 11 1.6 1.25 0.6 (48 cm) (24 cm) (28 cm) (28 cm) 22 10 13 11 3.3 2.5 1.3 (56 cm) (25 cm) (34 cm) (28 cm) 6.6 5.0 2.5

14.0 10.0 3.0 27 (68 cm) 12 (31cm) 18 (46 cm) 12 (31cm)

GERMANY New Brunswick Scientific GmbH In der Au 14 D-72622 Nrtingen Tel: +49 (0)7022-932490 Fax: +49 (0)7022-32486 E-Mail: [email protected]

24.5 (62 cm) 10.5 (27 cm) 16 11 (41 cm) (28 cm)

Drive Range Sensor AGITATION Control Impellers Indication OXYGEN TRANSFER RATE (OTR) Range TEMPERATURE Control Sensor 2-Gas Mode 4-Gas Mode AERATION

Permanent magnet top-drive motor with high torque. Readily removable without tools. 50 to 1000 rpm5 Photo-optical encoder disk with a resolution of 1000 pulses/revolution Microprocessor-based with PID Six-bladed Rushton impellers standard. Other impellers optional Digital display in 1 rpm increments 350 mM O2/L/Hr or more, for fermentation applications only From 5C above coolant temperature to 80C 0.1C, or from + 4C to 80C with optional refrigeration system, 0.1C. Rapid temperature shift 6 of 1C/minute (25C-45C) PID with pulse-width modulation of heater and cooling water Platinum RTD Air and O2 metered to ring sparger in fermentation mode Air, O2, CO2 and N2 for interactive pH/DO control in cell culture mode or manual dosing of gasses for plant cell culture

BELGIUM New Brunswick Scientific N.V./SA. t Veldeke 1 B-1970 Wezembeek-Oppem Belgi/Belgique Tel: +32 (0)2 731 6787 Fax: +32 (0)2 731 8130 E-Mail: [email protected] FRANCE New Brunswick Scientific S.A.R.L. 3 Rue des Deux-Boules F 75001 Paris France Tel: +33 (0)1 40 26 22 46 Fax: +33 (0)1 40 26 54 23 E-Mail: [email protected]

EXHAUST SYSTEM pH

Air Flow, Electronic Choice of Thermal Mass Flow Meter (TMM) or Thermal Mass Flow Controller (TMC) Sparger Removable Ring Sparger 0.2 m absolute filter cartridge interchangeable and repeatedly autoclavable Inlet Filter Filter 0.2 m absolute filter cartridge interchangeable and repeatedly autoclavable Condenser Stainless steel water-cooled condenser mounted in headplate minimizes evaporation Setpoint/Display Range 2.00 to 12.00 Fermentation: PID control of liquid additions. Cell Culture: 4-gas interactive Control Modes pH/DO control; and base addition with optional BioCommand software Probe Setpoint Range Display Range Select Ingold or Broadley James gel or Ingold liquid-filled probes 0 to 100% 0.1 0 to 200% 0.1 Fermentation: PID serial and parallel cascades to agitation and O2 gas, (and to air flow with TMC only). Cell Culture: 4-gas interactive pH/DO Select Ingold or Broadley James probes 5 assignable pumps provided for control of nutrient, acid, base, foam, harvest and 3 different levels 3. Control output for optional sixth pump also provided 0 to 100% of output 25.5 (65 cm) 24.75 (63 cm) 30.0 (76 cm) 380 lbs. (172 kg) 390 lbs. (177 kg) 340 lbs. (154 kg) 350 lbs. (159 kg)

THE NETHERLANDS New Brunswick Scientific B.V. Kerkenbos 1101, 6546 BC Nijmegen P.O. Box 6826, 6503 GH Nijmegen Tel: +31 (0)24 3717 600 Fax: +31 (0)24 3717 640 E-Mail: [email protected]

UNITED KINGDOM New Brunswick Scientific (UK) Ltd. Edison House, 163 Dixons Hill Rd. North Mymms, Hatfield, Herts AL9 7JE Tel: +44 (0) 1707 275733 Fax: +44 (0) 1707 267859 E-Mail: [email protected]

DO

Control Modes Probe

PUMPS

Control Indication

CHINA No. 9A, Ai Bang Building 585, Ling Ling Rd. Shanghai 200030, P.R. China Tel: 86-21-64812658 Fax: 86-21-64812665 E-Mail: [email protected]

OVERALL DIMENSIONS4 WEIGHT APPROXIMATE ELECTRICAL DATA

Width Front-to-back Height Gross Net Fuse Power

372 lbs. (169 kg) 332 lbs. (150 kg)

465 lbs. (211 kg) 405 lbs. (184 kg)

DATA DISPLAY SCREEN COMMUNICATIONS PORT RECORDER OUTPUT

12 Amps for 100V & 120V, 6.25 Amps for 220V & 240V Accepts 100 - 240V 50 or 60 Hz service 8 (20.3 cm) alpha-numeric vacuum-fluorescent screen displays multiple lines of data using 40 characters for up to 13 control loops Compatible with both EIA-422A and 232C standards 4-channel output for pH, DO, temperature and agitation

(1) Specifications subject to change without notice. For optional cell culture accessories and vessel dimensions, see our CelliGen Plus literature. (2) Vessel dimensions shown are as set up for an autoclave, with the motor removed. Slant racks are available that reduce height requirements. (3) Different liquid levels may be employed to trigger pumps for delivery of antifoam and nutrient or for harvest of culture when liquid level reaches a pre-set volume. (4) Allowances should be made for additional space required for hose and electrical connections on both sides of the console, in addition to 4 (10 cm) of clearance required in rear of unit. (5) Agitation to 1,200 rpm maximum is possible. Consult with NBS. (6) Rapid temperature shifts applicable in vessels up to 5L working volume only. For 10L vessels, contact NBS.

New Brunswick Scientific Co., Inc.

B-4010

P.O. Box 4005 44 Talmadge Road Edison, NJ 08818-4005 USA Phone: 800-631-5417 732-287-1200 Fax: 732-287-4222 E-Mail: [email protected] Internet: http://www.nbsc.com

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