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DBT Project

This document lists consumables and reagents needed for a DBT project studying the role of TG2 on Ank mediated mineralization of articular cartilage. It includes a list of 30 items, such as nitrogen cylinders, culture dishes, primers for various genes, DMSO, collagenase, FBS, RNA reagents, cDNA synthesis kits, PCR reagents, an enzymatic assay kit, and siRNA reagents for Ank and TG2 genes. The project requires materials for cell culture, RNA extraction and analysis, PCR, and transfection studies.

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Shahin Fatima
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35 views2 pages

DBT Project

This document lists consumables and reagents needed for a DBT project studying the role of TG2 on Ank mediated mineralization of articular cartilage. It includes a list of 30 items, such as nitrogen cylinders, culture dishes, primers for various genes, DMSO, collagenase, FBS, RNA reagents, cDNA synthesis kits, PCR reagents, an enzymatic assay kit, and siRNA reagents for Ank and TG2 genes. The project requires materials for cell culture, RNA extraction and analysis, PCR, and transfection studies.

Uploaded by

Shahin Fatima
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOC, PDF, TXT or read online on Scribd
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DBT PROJECT (Role of TG2 on Ank mediated mineralization of articular cartilage) CONSUMABLES 1. Nitrogen Cylinder 2.

100mm Tissue Culture Dishes 3. (6) well culture plates 4. Serological pipettes 5. Steripette 6. Cryovials 7. Glass Petri dishes 8. Cell scraper 9. Test tube stands 10. Wash bottles 11. Muslin cloth 12. Pipettes PRIMERS (forward + reverse) 1. Ank 2. TG2 3. GaPDH 4. Collagen Type I 5. Collagen Type II 6. Collagen Type X 7. Annexin II 8. Annexin V 9. Annexin VI 10. MMP13 11. Cbfa1 12. APase 13. HLA G isoforms

DBT REAGENTS/CHEMICALS 1. Dimethyl sulphoxide (DMSO) 2. Cetyl Pyridinium Chloride 3. Sodium phosphate 4. Alizarin Red S 5. Collagenase 6. Vitamin A acetate powder 7. Dulbeccos Modified Eagles Medium (DMSO) 8. Phosphate buffered saline (PBS) 9. Trizol reagent 10. Fetal bovine serum 11. Trypsin EDTA 12. Antibiotic mix 13. DEPC 14. Isopropanol 15. Chloroform 16. RNA Zap wipes 17. RNA later solution 18. RNA Zap solution 19. DEPC treated tips 20. cDNA synthesis kit-ABI 21. cDNA synthesis kit-Fermentas 22. 2X PCR Master mix 23. DNA Taq Polymerase 24. Nuclease Free Water 25. Taq Buffer 26. dNTPs 27. Enzcheck Pyrophosphate Assay Kit 28. siRNA Ank specific 29. siRNA TG2 specific 30. Lipofectamine Transfection Reagent

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