Scribd
Upload
8K views3 pages

HOW TO - Calculate KM and Vmax With Excel

This document provides step-by-step instructions for using Microsoft Excel and the Solver add-in to calculate the kinetic parameters Km and Vmax by fitting experimental enzyme reaction rate data. The user inputs substrate concentration and reaction rate data, then uses the Solver to adjust the values of Km and Vmax cells to minimize the difference between calculated and experimental rates. When the target cell showing this difference is minimized, the optimal values of Km and Vmax have been determined that best fit the Michaelis-Menten equation to the experimental data.

Uploaded by

minjeshp
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
8K views3 pages

HOW TO - Calculate KM and Vmax With Excel

This document provides step-by-step instructions for using Microsoft Excel and the Solver add-in to calculate the kinetic parameters Km and Vmax by fitting experimental enzyme reaction rate data. The user inputs substrate concentration and reaction rate data, then uses the Solver to adjust the values of Km and Vmax cells to minimize the difference between calculated and experimental rates. When the target cell showing this difference is minimized, the optimal values of Km and Vmax have been determined that best fit the Michaelis-Menten equation to the experimental data.

Uploaded by

minjeshp
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
You are on page 1/ 3

2/8/12 HOW TO: Calculate Km and Vmax with Excel

1/3 src.sfasu.edu/avk/BTC560/HOW TO.htm


HOW TO: Calculate Km and Vmax with Excel
Summary
This step by step article explains how to calculate Km and Vmax using Microsoft Excel.
Solver add-in is available as a part of optional component in Excel. With Solver, you can find an optimal value for a formula in one
cell called the target cell on a worksheet. Solver works with a group of cells that are related, either directly or indirectly, to
the formula in the target cell. Solver adjusts the values in the changing cells you specify called the adjustable cells to produce
the result you specify from the target cell formula. Your target formula of course will be the Michaelis-Menten formula. You will use
it to calculate the calculated rates. You will need the values of Km and Vmax which will be your adjustable cells and since we
dont know the values we will use random numbers. The target cell will be the sum of the square differences between experimental
and calculated rates. When the target cell will be minimized it will minimize the difference between the experimental and the
calculate rate and adjust the values of Km and Vmax thus fitting the calculated rates curve to your experimental data.
Requirement
Excel 2000 or 20002
Solver to add the Solver to your Tools click on the Tools menu select Add-ins and then selecting Solver from the Add-ins
window. The Solver becomes available in the Tools menu.
Substrate concentration
Experimental Rates or Velocities calculated for every substrate concentration used
Calculating velocity with preset Km and Vmax
Select cell A1 and type Km= and press enter. Type Vmax= and then select cell B1. The B1 will contain the value for Km as
mentioned above we will use a random number so type 1 and press enter. Type 1 again and this will be your Vmax. If you fill your
data similar to the next picture then you can follow the step by step instructions.
To calculate the Calculated velocity select the C5 and type = then select the value of Vmax (cell B2), type *, select the
corresponding substrate (cell A5), type /, open parenthesis, select the value of Km (B1), add the substrate concentration and close
the parenthesis. To keep the position of the Km and Vmax values constant add the $ sign before the column and the row number
so instead of B2 you will have $B$2. Hit enter. Select the calculated cell and move the mouse pointer to the right bottom corner,
when the pointer turns into a cross push the mouse left button and pull it down to calculate the rates for the rest of the substrate
concentrations.
2/8/12 HOW TO: Calculate Km and Vmax with Excel
2/3 src.sfasu.edu/avk/BTC560/HOW TO.htm
Plotting eperimental and calculated data on one plot
T iaie he daa i bh eie e
Seec he dae c A C ad cedig deedig h a bae cceai hae ad hi he
b ba ,eec cha e caeed ad cic e. F he e f he cha iad e fiih he
if ih eae he eie a d . Oce he i ead dbe cic he daa eie ha ced cacaed
eci (he cic he daa eie he cedig c ad ae highighed) ad chage he ae fa
e ad ie fa c. Y hd iia he be.
Calculating difference
Cacae he diffeece beee he eaed ad eeiea eciie. Y ca e he ece fa POWER cacae he
ae f he diffeece. Y cee i iia he fig ice.
Sice he Se ca iiie e ae a a ie i cacae he f he ae f diffeece.
2/8/12 HOW TO: Calculate Km and Vmax with Excel
3/3 src.sfasu.edu/avk/BTC560/HOW TO.htm
Calculating Km and Vma
Seec he ce hich cai he f he ae diffeece ad che e f he T e.
Y cee i iia he fig ice.
A ca ee he Tage ce i e he f he ae diffeece ad i i be iiied b chagig he ce hich cai
he ad ae f K ad Va.
Seec Se, e i e afe fi aiai ad i eed cic cie b. Whe he a ieai i de
i e ha he cacaed ce if fi eaed eciie ad he K ad Va ae chaged.
T e he gde f he fi a a addiia eecie ca cacae he ceai cefficie R-ae.

You might also like