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Thin Layer Chromatographic Method For Determining Plant Pigments in Marine Particulate Matter, and Ecological Significance of The Results

This document describes a thin layer chromatographic method for determining plant pigments in marine particulate matter. The method involves filtering sea water through a glass fiber filter to collect particulate matter, extracting plant pigments from the particulate matter using 90% acetone, and separating the pigments using thin layer chromatography. Nine types of pigments can be fractionated using this method. Analysis of sea water samples from the central Pacific showed that chlorophyll a levels peaked at depths of 100-150m and decreased with depth, chlorophyll a made up over 60% of total pigments even at depths of 1000m, and phaeophytin a was absent from the samples examined.

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71 views15 pages

Thin Layer Chromatographic Method For Determining Plant Pigments in Marine Particulate Matter, and Ecological Significance of The Results

This document describes a thin layer chromatographic method for determining plant pigments in marine particulate matter. The method involves filtering sea water through a glass fiber filter to collect particulate matter, extracting plant pigments from the particulate matter using 90% acetone, and separating the pigments using thin layer chromatography. Nine types of pigments can be fractionated using this method. Analysis of sea water samples from the central Pacific showed that chlorophyll a levels peaked at depths of 100-150m and decreased with depth, chlorophyll a made up over 60% of total pigments even at depths of 1000m, and phaeophytin a was absent from the samples examined.

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Geochemical Journal, Vol. 5, pp.

23 to 37, 1971

Thin layer chromatographic method for determining plant pigments in marine particulate matter, and ecological significance of the results

KATSUMI YANAGI and TADASHIRO KOYAMA

Water Research Laboratory, Faculty of Science, Nagoya University, Chikusa, Nagoya, Japan (Received February 13, 1971; in revised form July 3, 1971) Abstract -Thirty to 100 liters of sea water sample containing MgCO3 powder was filtered through a glass fiber filter in order to collect particulate matter. Soon after the filtration, this glass fiber filter was kept in a deep freezer ( -20C) for later use. The following pigment analysis was carried out within a week. Plant pigments in the collected particulate matter were extracted with 90% acetone in a dark refrigerator. Pigments in the acetone extract were separated by thin layer chromatography using a mixture of cellulose powder and soluble starch as an adsorbent. The developing solvent was 1.2% n -propanol in petroleum ether. According to this procedure, 9 kinds of pigments were fractionated. Each of the fractionated pigments was eluted with 90% acetone and determined by measuring fluorescence of the acetone extracts after acidification with a small amount of hydrochloric acid . The analyses of sea water samples from the central Pacific showed the following facts: (1) Vertical distribution of chlorophyll a had a peak at a depth of 100m or 150m and its amount remarkably decreased with depth in parallel with the total pigments. (2) The percentage of chlorophyll a in the total pigments was more than 60% for the sea water samples examined, even at the depth of 1,000m. (3) The absence of phaeophytin a was made clear for the samples examined,

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