Scribd
Upload
67 views6 pages

Lecture 16

The pentose phosphate pathway occurs in the cytosol and consists of an oxidative and nonoxidative phase. The oxidative phase generates NADPH which is used for reductive biosynthesis. The nonoxidative phase interconverts 5-carbon sugars to produce ribose-5-phosphate for nucleotide biosynthesis and glycolytic intermediates. Key enzymes in the oxidative phase include glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase. The pentose phosphate pathway provides NADPH for fatty acid, cholesterol and neurotransmitter biosynthesis as well as glutathione reduction. Tissues with active pentose phosphate pathways include the liver, adipose tissue and red blood cells.

Uploaded by

lissandr
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
67 views6 pages

Lecture 16

The pentose phosphate pathway occurs in the cytosol and consists of an oxidative and nonoxidative phase. The oxidative phase generates NADPH which is used for reductive biosynthesis. The nonoxidative phase interconverts 5-carbon sugars to produce ribose-5-phosphate for nucleotide biosynthesis and glycolytic intermediates. Key enzymes in the oxidative phase include glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase. The pentose phosphate pathway provides NADPH for fatty acid, cholesterol and neurotransmitter biosynthesis as well as glutathione reduction. Tissues with active pentose phosphate pathways include the liver, adipose tissue and red blood cells.

Uploaded by

lissandr
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
You are on page 1/ 6

PENTOSE PHOSPHATE

PATHWAY

JULIUS P. MARIO, RMT, MS


PENTOSE PHOSPHATE PATHWAY
 Occurs in the cytosol
 Meets the need of all organisms for a source
of NADPH to use in reductive biosynthesis
 consists of two phases:
Oxidative generation of NADPH when glucose-6-
phosphate is oxidized to ribose-5-phosphate
Nonoxidative interconversion of 3-, 4-, 5-, 6- and 7
carbon sugars to synthesize five-carbon sugars
for two purposes:
 Biosynthesis of nucleotides
 Production of excess into glycolytic pathway intermediates
Glucose-6-phosphate
PHASE 1
G-6-PD, Lactonase,
6-Phosphogluconate 2 NADP+
(Oxidative) dehydrogenase
2 NADPH
Ribulose-5-phosphate
Phosphopentose
isomerase Phosphopentose Phosphopentose
epimerase epimerase
Ribose-5-phosphate
Xylulose-5-phosphate
Xylulose-5-phosphate
Transketolase

GAP Sedoheptulose-7-phosphate

Transaldolase

Fructose-6-phosphate Erythrose-4-phosphate

Transketolase
PHASE 2
Fructose-6-phosphate GAP
(Nonoxidative)
REACTION ENZYME
Oxidative Phase
G-6-P + NADP+  6-phosphogluconolactone + NADPH + H+ G-6-PD
6-phosphogluconolactone + HOH  6-phosphogluconate + H+ Lactonase
6-phosphogluconate + NADP+ ribulose-5-phosphate + NADPH + CO2 6-Phosphogluconate

dehydrogenase
Nonoxidative Phase
Ribulose-5-P  Ribose-5-P Phosphopentose
isomerase
Ribulose-5-P  Xylulose-5-P Phosphopentose
epimerase
Xylulose-5-P + Ribose-5-P  Sedoheptulose-7-P + Transketolase
C5 + C5 ------ C7 + C3 Glyceraldehyde-3-
P
Sedoheptulose-7-P + glyceraldehyde-3-P  Fructose-6-P + Transaldolase

C7 + C3 +
Xylulose-5-P ----- C6 + 
Erythrose-4-P C4 Fructose-6-P + Erythrose-4-P Transketolase

C5 + C4 ----------- C6 + C3 Glyceraldehyde-3-
P
PATHWAYS REQUIRING NADPH
 BIOSYNTHESIS
 Fattyacid
 Cholesterol
 Neurotransmitter
 Nucleotide

 DETOXIFICATION
 Reductionof oxidized glutathione
 Cytochrome P450 monooxygenases
TISSUES WITH ACTIVE PENTOSE
PHOSPHATE PATHWAYS
TISSUE FUNCTION

 Adrenals Steroid synthesis


 Liver Fatty acid & Cholesterol synthesis
 Testes Steroid synthesis
 Adipocytes Fatty acid synthesis
 Ovary Steroid synthesis
 Mamma Fatty acid synthesis
 RBC Maintenance of reduced glutathione

You might also like