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Lab Report

This lab explored how temperature affects enzyme activity. Students measured the decomposition of hydrogen peroxide by potato enzymes over 5 minutes at 10°C, 30°C, and 80°C. Results showed enzyme activity was fastest at 10°C and slowest at 80°C, demonstrating that colder temperatures optimize an enzyme's ability to catalyze reactions.

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mercedesbenz1996
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53 views

Lab Report

This lab explored how temperature affects enzyme activity. Students measured the decomposition of hydrogen peroxide by potato enzymes over 5 minutes at 10°C, 30°C, and 80°C. Results showed enzyme activity was fastest at 10°C and slowest at 80°C, demonstrating that colder temperatures optimize an enzyme's ability to catalyze reactions.

Uploaded by

mercedesbenz1996
Copyright
© Attribution Non-Commercial (BY-NC)
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Villeneuve 1 Introduction Enzymes are a type of biological molecule called a protein. Enzymes are made of amino acids.

In living cells, enzymes speed up the rate of chemical reactions in the biological process. The only reason that substrates exist are because of enzymes. The purpose of this lab was to learn how the temperature around the enzyme affects its activity. Methods In this lab, the first thing we had to do was gather our materials, a test tube, pieces of potato, a tube test rack, and a few different beakers. Next we put a piece of potato in the test tube and added 4ml of a 3% hydrogen peroxide solution. We had to gently tap the test tube to mix the potato and the peroxide, then began timing. Every minute for five minutes, we measured how high the foam was. Then we repeated those steps, but this time we put the test tube in 10 degree, 30 degree, and 80 degree water. The units we used were centimeters (for measuring the foam), and milliliters (for measuring the liquids). Results
Height Of Foam Over Time Air Temperature

1 min
.5cm 1.6cm 1.5cm .1cm

2 min
.8cm 2cm 2.2cm .4cm

3 min
.8cm 2.4cm 2.8cm .9cm

4 min
1.1cm 2.9cm 3cm 1.1cm

5 min
1.3cm 3.1cm 3.4cm 1.1cm

10 c 30 c 80 c

The graph above shows the results from the lab after each minute with the according temperature. The 80 degree water made the enzyme much slower than it was with normal air/room temperature. Enzyme activity was the fastest with the 10 degree water. Discussion The lab helped and taught us how the temperatures around the enzyme will affect its activity. The enzyme reacts fastest in colder surroundings. My classmates and I did not have differing results. The only sources of error were that some of the directions could have been clearer.

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