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Flow Charts

The document outlines flow charts for categorizing different types of gram-positive cocci and gram-negative rods based on their reactions to various biochemical tests. For gram-positive cocci, it distinguishes between catalase-negative and catalase-positive varieties and provides subsequent tests to differentiate between streptococci, enterococci, staphylococci, and micrococci. For gram-negative rods, it separates those that are lactose fermenting from those that are not and outlines additional tests like oxidase, gelatin, urea, H2S production, and motility to identify organisms like E. coli, Klebsiella, Pseudomonas, Proteus, Salmonella, and

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Budiyanto Prasetyo
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2K views4 pages

Flow Charts

The document outlines flow charts for categorizing different types of gram-positive cocci and gram-negative rods based on their reactions to various biochemical tests. For gram-positive cocci, it distinguishes between catalase-negative and catalase-positive varieties and provides subsequent tests to differentiate between streptococci, enterococci, staphylococci, and micrococci. For gram-negative rods, it separates those that are lactose fermenting from those that are not and outlines additional tests like oxidase, gelatin, urea, H2S production, and motility to identify organisms like E. coli, Klebsiella, Pseudomonas, Proteus, Salmonella, and

Uploaded by

Budiyanto Prasetyo
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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Gram-positive Cocci

Gram-positive Cocci
Catalase Test

Catalase Negative Flow Chart

Streptococcaceae Hemolysis

+
Micrococcaceae

Beta
GpC Strep GpA Strep (S. pyogenes)

Alpha
Enterococcus Strep mitis

Bacitracin Sensitivity

Bile Esculin Test

+
GpA Strep (S. pyogenes)

GpC Strep

+
Enterococcus

Strep mitis Strep pneumoniae

Optichin Sensitivity

+
Strep pneumoniae

Strep mitis

Gram-positive Cocci

Gram-positive Cocci

Catalase Test

Catalase Positive Flow Chart


+
Micrococcaceae

Streptococcaceae

Coagulase Test

Mannitol Fermentation Test

Hemolysis

+
Staphylococcus aureus

Staphylococcus epidermidis Micrococcus luteus Micrococcus roseus

+
Staphylococcus aureus

Staphylococcus epidermidis Micrococcus luteus Micrococcus roseus

Beta
Staphylococcus aureus

Alpha
Staphylococcus epidermidis Micrococcus luteus Micrococcus roseus

Growth on Mannitol Salt Agar Test (MSA)

Pigmentation Observation

Growth on MSA or Pigmentation Observation

+
Staphylococcus aureus Staphylococcus epidermidis

Micrococcus luteus Micrococcus roseus

Grey/White
Staphylococcus epidermidis

Golden/White
Staphylococcus aureus

Mustard Yellow

Pale Pink/Rose

Micrococcus luteus

Micrococcus roseus

See Pigmentation Observation to differentiate

Gram-negative Rods

Gram-negative Rods

Lactose Positive Flow Chart


+

Lactose Test
(KIA or Carbohydrate Fermentation Test)

IMViC Tests

++-E. coli

--++

Motility

+
Enterobacter aerogenes

Klebsiella

Gram-negative Rods

Gram-negative Rods

Lactose Test
(KIA or Carbohydrate Fermentation Test)

Lactose Negative Flow Chart


_

Oxidase Test

Gelatin Test

H2S (KIA) Test

+ Pseudomonas aeruginosa

Alcaligenes faecalis

Shigella flexneri

Urea Test

+ Proteus vulgaris

Salmonella typhimurium

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