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    Manual Sample Orange "Celmaxpro™" Stickers On Kit and Birth Report Axp Sample No Sticker

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    audwong
    The document provides instructions and specifications for manual and automated processing of umbilical cord blood samples, including spin speeds and durations, temperature settings, sample volume ranges, mass measurements of collection bags, cell count targets, and procedures for adding chemicals and taking blood counts. It also provides instructions for removing positive and negative samples from the BACTEC machine and running self tests on the device.

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    Attribution Non-Commercial (BY-NC)
    Download as DOC, PDF, TXT or read online from Scribd

    Manual Sample Orange "Celmaxpro™" Stickers On Kit and Birth Report Axp Sample No Sticker

    Uploaded by

    audwong
    23 views8 pages
    The document provides instructions and specifications for manual and automated processing of umbilical cord blood samples, including spin speeds and durations, temperature settings, sample volume ranges, mass measurements of collection bags, cell count targets, and procedures for adding chemicals and taking blood counts. It also provides instructions for removing positive and negative samples from the BACTEC machine and running self tests on the device.

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    The document provides instructions and specifications for manual and automated processing of umbilical cord blood samples, including spin speeds and durations, temperature settings, sample volume ranges, mass measurements of collection bags, cell count targets, and procedures for adding chemicals and taking blood counts. It also provides instructions for removing positive and negative samples from the BACTEC machine and running self tests on the device.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    23 views8 pages

    Manual Sample Orange "Celmaxpro™" Stickers On Kit and Birth Report Axp Sample No Sticker

    Uploaded by

    audwong
    The document provides instructions and specifications for manual and automated processing of umbilical cord blood samples, including spin speeds and durations, temperature settings, sample volume ranges, mass measurements of collection bags, cell count targets, and procedures for adding chemicals and taking blood counts. It also provides instructions for removing positive and negative samples from the BACTEC machine and running self tests on the device.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    of 8

    Manual Orange CelMaxPro stickers on kit sample and birth report AXP sample No sticker

    Manual sample: 1st spin 2nd spin Blood tubes

    Speed (rpm) 510 1900 3000

    Duration (min) 8 10 10

    Temp (TC) 10 10 10

    Chill sample and DMSO Dextran at 4C for 15 minutes before injecting.


    Sample type Manual AXP SMS format To: Chris Ong, UCB Tan Moi Yen= 75.2ml, 556.0 million cells. Audrey out. DMSO volume (ml) 6 7

    Mass of ONE (1) maternal tube = 7.3g

    Before starting CRF, Equipment CRF LN2 Reminder switch is on - check level - opened

    Normal volume sample Small volume sample Large volume sample

    40ml 99.9ml < 40ml > 100ml

    AXP UCB processing range: 25 135ml Out of this range: processed manually

    Mass of collection bag + CPDA

    Name of bag TERUMO JMS JMS + injection port

    Mass (g) 56 58 64

    Ensure correct specimen type and lot number.

    Abort processing if T.N.C. < 200 million cells.

    Cell viability
    Incubation time: 5-10 minutes

    To remove negative BACTEC vials:

    Record negative sample number in BACTEC file

    Scan Remove NEGATIVES

    Green LED lights up

    Remove vials

    To remove positive BACTEC vials: Record positive sample number in BACTEC file

    Check paper

    Press F4 and key in sample position Press F10

    Press F4 again. Then press Print Screen

    Scan Remove POSITIVES

    Amber LED lights up

    Remove vial and scan barcode

    Send positive sample to Gribbles

    Device Self Test


    Program 3

    Amber light blinking?

    Yes

    No

    Program 4

    Green light blinking?

    Yes

    No

    Done

    Spinning samples = Program 10

    Note:

    Program no. 1 2 3 4 10

    Speed (rpm) Duration (min)

    Temp (C)

    1400 20 22 85 10 22 1400 5 22 85 5 22 Auto change from program 1 to program 2

    ABO test Sample type Control Sample Volume to draw (l) 25 20

    Control for ID Newborn Card must always be negative.

    Pre-processing Sample volume range Small Normal Large PBSC empty collection bag Volume to draw (l) 1 3 11

    Bag type COBE HAEMONITICS Cell-Dyn blood count dilution Factor 2x 3x 4x 5x Chemical dilution Chemical 10% cholorox 70% EtOH 1% plasmalyse Passing range AXP Manual T.N.C. (%) 60-110 60-110 R.O volume (ml) 2700 300 45 Diluent (l) 200 400 300 400

    Mass (g) 40 30

    Sample (l) 200 200 100 100

    Chemical volume (ml) 300 700 5

    M.N.C. (%) 75-100 N/A

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