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RFLP

RFLP analysis measures variations in lengths of DNA fragments containing short repeated sequences called VNTRs. DNA from samples is amplified, cut with restriction enzymes, and fragments are sorted by length using gel electrophoresis. If samples have VNTRs of different lengths, they cannot be from the same person, but if lengths match, they could be from the same person or two people with identical VNTR lengths. Comparing many VNTRs reduces the chance of coincidental matches to nearly zero. RFLP is time-consuming, taking weeks to complete, and has been replaced by faster techniques.

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223 views1 page

RFLP

RFLP analysis measures variations in lengths of DNA fragments containing short repeated sequences called VNTRs. DNA from samples is amplified, cut with restriction enzymes, and fragments are sorted by length using gel electrophoresis. If samples have VNTRs of different lengths, they cannot be from the same person, but if lengths match, they could be from the same person or two people with identical VNTR lengths. Comparing many VNTRs reduces the chance of coincidental matches to nearly zero. RFLP is time-consuming, taking weeks to complete, and has been replaced by faster techniques.

Uploaded by

achin47
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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RFLP

Restriction Fragment Length Polymorphism (RFLP) analysis If two different samples show VNTRs of different lengths, the
measures fragments of DNA containing short sequences that samples could not have come from the same person. On the other
vary from person to person, called VNTRs. After extracting DNA hand, two samples showing VNTRs of the same length could have
from a sample and amplifying it with the technique known come from the same person, or from two people who happen to
as Polymerase Chain Reaction (See page 4), a technician have VNTRs of the same length at that location. By comparing
adds restriction enzymes that cut the DNA at specific points. enough VNTRs from two individuals, however, the likelihood of
The resulting fragments can be sorted by length with gel a coincidental match can be reduced to nearly zero. RFLP testing
electrophoresis technology to determine how many times a given requires hundreds of steps and weeks to complete, and it has
VNTR is repeated. been largely replaced by newer, faster techniques.

1. DNA Purified

Sample 1 Sample 2

2. DNA Fragmentation

Sample 1
Restriction enzyme cuts DNA

G T C C A T G ATTAC
GC T A G C A T A C C T A G T A A G A T C C G A C TA A A T C C A G C C G G G C A T C C A T C C A T G C G T A A T

T C C TA G C ATA C C TA G TA A G A T C C G A C TA A A T C C A G C C G G G C AT C C A

Three fragments

Sample 2
Restriction enzyme cuts DNA
G T C C A T G ATTAC
GC T A G C A T A C C T A G T A A G G A T C C T A G T A A T C C A C C T A G T A A G G T A C T G A T C C T A A G G T A C

T C C TA G C ATA C C TA G TA A G G AT C C TA G TA A T C C A C C TA G TA A G G TA C T G A

Two fragments

3. Gel Electrophoresis Sample


sizing
standard 1 2
increasing size

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