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Mr. Shardul S. Wagh

1. Enzymes are protein biocatalysts that increase the rate of chemical reactions in living cells without being consumed in the process. 2. There are several systems used to name and classify enzymes, including by substrate, reaction type, IUB classification, and chemical composition. 3. The IUB system categorizes enzymes into 6 main classes based on reaction type, with additional subclasses and enzyme numbers to further specify each enzyme. It provides a standardized naming system for enzymes.

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Shardul Wagh
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806 views

Mr. Shardul S. Wagh

1. Enzymes are protein biocatalysts that increase the rate of chemical reactions in living cells without being consumed in the process. 2. There are several systems used to name and classify enzymes, including by substrate, reaction type, IUB classification, and chemical composition. 3. The IUB system categorizes enzymes into 6 main classes based on reaction type, with additional subclasses and enzyme numbers to further specify each enzyme. It provides a standardized naming system for enzymes.

Uploaded by

Shardul Wagh
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© Attribution Non-Commercial (BY-NC)
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Download as PPT, PDF, TXT or read online on Scribd
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Mr. Shardul S.

Wagh
[email protected]
Introduction:
Enzymes are biocatalysts. They increase the rate of chemical
reaction taking place within living cells without themselves
suffering any overall change.

The name ‘enzyme’ (enG = in; zymeG = yeast) means ‘in yeast’

•Enzymes are protein in nature.


•They enhance the rate of reaction by lowering the activation
energy.
•pH and temperature sensitive.
•Specific in nature.
Enzyme exploration was influenced by modern scientific
approach of fermentation industry.

The name ‘enzyme ’ was


coined by observing
conversion of fruit juice
(sugar) in to fine wine (alcohol)
and release of Co2
How Enzymes work?????????
The reactants of enzyme –catalyzed reaction are termed substrates
and each enzyme is quite specific in character, acting on a
particular substrate to produce a particular product or products.
[E] + [S] [ES] [P] + [E]
Complexity of Biochemical Pathways
C
om
pl
ex
i ty
Pa o
th f B
w io
ay c
s he
m
ic
al
How to remember and name these
enzymes??????
Enzyme Nomenclature and Classification
With continuous increase in knowledge of enzymology, various
systems have evolved to name and classify the enzymes.

1. Substrate acted upon by the enzyme.


2. Type of reaction catalyzed.
3. Substrate acted upon and type of reaction catalyzed.
4. Substrate that is synthesized.
5. Chemical composition of the enzyme.
6. Substance hydrolyzed and group involved.
7. I.U.B. classification.
1. Substrate acted upon by the enzyme.

The substance upon which an enzyme acts is called the substrate.


Duclaux (1883) named the enzyme by adding the suffix – ase in the
name of the substrate catalyzed.

Examples:
Carbohydrate – Carbohydrase
Protein – Proteinase
Lipid – Lipase
Maltose – Maltase
Sucrose – Sucrase
2. Type of Reaction catalyzed.

 The enzymes are highly specific as to the reaction they catalyzed.


 Hence this classification is based on adding suffix – ase in the
name of reaction they catalyzed.
Examples:
Hydrolase – hydrolysis.
Isomerases – isomerization.
Oxidase – oxidation.
Dehydrogenases – dehydrogenation.
Transaminases – transamination.
3. Substrate acted upon and type of reaction
catalyzed.

The name of some enzymes give clue of both the substrate utilized
and the type of reaction catalyzed.

Eg.
Succinic dehydrogenase :- catalyzes dehydrogenation of
substrate succinic acid.
L– glutamic dehydrogenase :- dehydrogenation of L –glutamic
acid.
4. Substrate that is synthesized.

Few enzymes named by adding the suffix - ase to the name of


the sustenance synthesized.

Eg.
Fumarase :- forms fumarate irreversibly by L – malate.
5. Chemical composition of the enzyme.

Classification based on chemical composition.

Examples:
 Enzyme containing protein only :- Pepsin, Trypsin, Urease.
 Enzyme containing protein & cation :- carbonic anhydrase.( Zn2+)

 Enzyme containing protein & non protein:- (Prosthetic group)

Iron porphyrin enzyme:- Catalase, Cytochrome c


peroxidase I & II
6. Substance hydrolyzed and group involved.

Examples:
Carbohydrate :- Cellulase, Amylase, Sucrase, - glucorinidase
Protein hydrolyzing enzymes :- Endopeptidases, exopeptidase.
Lipid:- Lipase, Esterase
7. I.U.B. Classification 1961

The chemical reaction catalyzed is specific property which


distinguishes one enzyme from another.
I.U.B. used this criterion as a basis for classification and
naming of enzymes.
The major features of this system are:-
Reactions are divided in to 6 major classes. Each with 4 to 13
subclasses.
1. Each enzyme has 2 parts – the first part is the name of substrate &
second with suffix – ase, indicate type of reaction catalyzed.
2. Additional information regarding nature of reaction.
Eg. Malate dehydrogenase. (L- malate : NAD oxidoreductase.)
L- Malate +NAD+ Pyruvate +Co2 +NADH+H+
I.U.B. Classification contd…

4.Each enzyme has been allotted systemic code called


Enzyme commission number (E.C.) it consist of series
of 4 digits.

Example. Alcohol Dehydrogenase. (E.C.1.1.1.1.)

1.1.1.1.
Class

Sub Class
(acting on CH – OH group of electron donor)

Sub Sub Class (electron acceptor)

Enzyme Number
Class 1. Oxidoreductase (Oxidation n Reduction)
Class 2. Transferase (Transfer of Group)
Class 3. Hydrolase (Hydrolysis)
Class 4. Lyases (Lysis)
Class 5. Isomerases (Isomers)
Class 6. Ligases (To joint)
Class 1. Oxidoreductase (Oxidation n Reduction)

These enzymes catalyze the transfer of H atoms, O- atoms or


electrons from one substrate to another.

Eg. 1.1.1.1. (Alcohol Dehydrogenase)

Enzyme serial Number.

Electron acceptor.

Acting on (CH – OH) alcohol group of electron donor.

Other example is Lactate Dehydrogenase (E.C. 1.1.1.27)


2. Transferase (Transfer of Group)
Catalyzes the reaction AX + B BX + A
While giving name to particular enzyme Enzyme commission
recommends that the name of transferase should end “X –
Transferase” where X – is the group transferred.

Eg. 2.1.1 methyltransferases ( - CH3) (transfer from first Carbon)

Eg. ATP : D- Hexose –6 – phosphatases (2.7.1.1) (Hexokinase)

D- hexose + ATP D – Hexose – 6 – (P) + ADP


Class 3. Hydrolase (Hydrolysis)
Enzymes catalyzes hydrolytic reaction.
A – X + H2 O X – OH + HA
These types of enzymes catalyzes the reaction of hydrolysis of ester,
glycosyl by addition of water molecule.

Eg. Orthophosphoric monoester phosphohydrolase (E.C. 3.1.3.1)

(Alkaline phosphatase)

Organic (P) inorganic (P)


Class 4. Lyases (Lysis)
These enzymes catalyses the non hydrolytic removal of groups
from substrates often leaving double bonds.

Eg. L – Histidine carboxy – lyase (E.C.4.1.1.22)


(Histidine – decarboxylase)
Histidine Histamine.
Class 5. Isomerases (Isomers)
Enzymes catalyzing isomerization reaction

Eg. Alanine racemase (E.C. 5.1.1.1)


L- alanine D – alanine.
Class 6. Ligases (To joint)

Enzymes catalyzed the synthesis of new bonds coupled to the


breakdown of ATP or other nucleoside triphosphates.

X + Y + ATP X – Y + ADP + Pi

E.C. 6.3.1. Acid - ammonia ligases.

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