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    Sds-Page 12 %

    Uploaded by

    Saravanan Jothi
    This document provides recipes for preparing SDS-PAGE gels and running a LeuDH assay. The SDS-PAGE gel recipes give amounts of water, acrylamide solution, buffer solution, APS, and TEMED needed for the resolving and stacking layers. The LeuDH assay lists sample and buffer abbreviations for running the assay.

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    Attribution Non-Commercial (BY-NC)
    Download as DOC, PDF, TXT or read online from Scribd

    Sds-Page 12 %

    Uploaded by

    Saravanan Jothi
    33 views1 page
    This document provides recipes for preparing SDS-PAGE gels and running a LeuDH assay. The SDS-PAGE gel recipes give amounts of water, acrylamide solution, buffer solution, APS, and TEMED needed for the resolving and stacking layers. The LeuDH assay lists sample and buffer abbreviations for running the assay.

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    SDS-PAGE 12 %

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    This document provides recipes for preparing SDS-PAGE gels and running a LeuDH assay. The SDS-PAGE gel recipes give amounts of water, acrylamide solution, buffer solution, APS, and TEMED needed for the resolving and stacking layers. The LeuDH assay lists sample and buffer abbreviations for running the assay.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    33 views1 page

    Sds-Page 12 %

    Uploaded by

    Saravanan Jothi
    This document provides recipes for preparing SDS-PAGE gels and running a LeuDH assay. The SDS-PAGE gel recipes give amounts of water, acrylamide solution, buffer solution, APS, and TEMED needed for the resolving and stacking layers. The LeuDH assay lists sample and buffer abbreviations for running the assay.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    of 1

    SDS-PAGE %

    Components Resolving Gel Stacking Gel


    Water 5.195 ml 3.295 ml
    Acrylamide 6.00 ml 670 μl
    4X Buffer 3.75 ml 1 ml
    APS 50 μl 30 μl
    TEMED 5 μl 5 μl
    TOTAL 15 ml 5 ml

    LeuDH Assay
    SUB
    EB
    SB
    TEST

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