429

Resins ................................................................................................ 430

Rezorian and Porozorb Cartridges ........................................................................ 430
Adsorption Media ................................................................................................ 431
HiTrap Cartridges ................................................................................................. 439
Affinity Media ...................................................................................................... 440
Gel Filtration Media ............................................................................................. 441
Hydrophobic Interaction Media ........................................................................... 443
Anion Exchange Media ........................................................................................ 444
Cation Exchange Media ....................................................................................... 449
Ion Exchange (Chelating) Media .......................................................................... 453
Ion Exchange (Mixed Bed) Media ........................................................................ 454
Ion Exchange (Nuclear) Media ............................................................................. 455
Ion Exchange Media ............................................................................................ 456
LPLC Columns and Accessories ............... .......................................... 457
Sigma-Aldrich Columns and Accessories .............................................................. 457
Omnifit Columns ................................................................................................. 458
Omnifit Accessories.............................................................................................. 458
Omnifit, Upchurch Accessories............................................................................. 461
Low Pressure Valves.............................................................................................. 463
Cheminert Plastic Fittings..................................................................................... 463
Cheminert Plastic Fittings & Tubing ..................................................................... 464
Tube Flanging Tool ............................................................................................... 465
Ultrafiltratio n ................................................................................... 466
Sartorius/Sartobind .............................................................................................. 466

SUPELCO
 430
Resins
Rezorian and Porozorb Cartridges

P000619
Rezorian A161 Cartridges
Disposable Rezorian A161 Luer lock syringe-tip cartridges offer
convenience and expedience for isolating, purifying, and
P000620
concentrating biomolecules from aqueous samples. Packed with Sterile
a Porozorb Cartridges
high performance, macroreticular, hydrophobic adsorbent resin Analysts processing protein or other biological preparations,
(Table 1), Rezorian A161 cartridges are specially tailored for sterile pharmaceuticals, foods, or beverages must separate wanted
biomolecular pharmaceutical separations. We can custom prepare products from unwanted process components. Produced using
Rezorian cartridges with a variety of resins - please inquire.
O r d e r : 1 . 8 0 0 . 3 2 5 . 3 0 1 0 Te c h n i c a l S e r v i c e : 1 . 8 0 0 . 3 5 9 . 3 0 4 1 We b : w w w. s i g m a - a l d r i c h . c o m / s u p e l c o

validated processes, Porozorb cartridges are sterile, endotoxin-

TABLE 1. RESIN CHARACTERISTICS: REZORIAN CARTRIDGES free, ready-to-use adsorbent cartridges that effectively remove
Particle Description: spherical styrene divinylbenzene, reagent detergents (Triton X-100, sodium dodecyl sulfate, Tween, etc.) or
grade other nonpolar, hydrophobic materials from such preparations.
Mean Particle Size: 120µm They are appropriate for analytical scale to process scale
Mean Pore Diameter: 110-175Å purification schemes.
Surface Area: 800-950m2/g
A certificate of analysis accompanies each Porozorb cartridge.
Cartridges are tested for Sterility and Endotoxin byaccredited
an
DESCRIPTION QTY. CAT. NO. PRICE
test labfollowing modified USP guidelines. The cartridges can be
Rezorian A161 Cartridges, 1mL 6 57610-U rinsed with cleaning agents (e.g., most weak acids and bases) or
Rezorian A161 Cartridges, 5mL 6 57611
autoclaved at 121°C. The polycarbonate cartridge can accept
50% organic solutions during analysis, but must be stored in
aqueous solutions.
RELATED INFORMATION
For additional information, request the following free literature by
phone or fax, or see our website. CHARACTERISTICS OF POROZORB CARTRIDGES 1

No. Subject Particle Description: spherical styrene/divinylbenzene, cleaned

T111871 Porozorb cartridges (purifying biological preparations) Mean Particle Size: 500µm
T494015 Rezorian cartridges
Resins & Media

Mean Pore Diameter: 40Å

T394001 Rezorian cartridges (concentrating ethidium bromide) Cartridge Dimensions: 1 6.55 x 8cm (250mL)
T394022 Rezorian cartridges (concentrating ethidium bromide) 26.20 x 8cm (1000mL)
42.04cm x 12.71cm (4000mL)
Nipple Connections: 3/16" ID, 1/4" OD
Shell: clear polycarbonate
Retaining Screens: stainless steel, 50x250 mesh,
highest quality
Gaskets: Melrath medical grade
Maximum Pressure: 30psi (2.1kg/cm 2)
Sterility: shipped sterile and endotoxin-free
1
We can make other Porozorb cartridges on request.

CARTRIDGE VOLUME CAT. NO. PRICE

POROZORB CARTIDGES
Porozorb 254 250mL 57500
Porozorb 1004 1000mL 57502
Porozorb 4004 4000mL 57513-U
NOTE: Porozorb cartridges are not for clinical or diagnostic use.
Due to shelf life limitations, Porozorb cartridges are made on receipt of an order.
Expect 2-4 week delivery times.
See Legally Speaking in the index.

SUPELCO
 431
Resins
Adsorption Media

Activated Alumina
Highly porous in nature, aluminas can be effective desiccants and
have numerous applications in catalysis. We offer four types of
activated alumina (Al2O3) designed for column chromatography:
acidic, weakly acidic, basic, and neutral. All grades have a
Brockmann activity of I. Prepare Brockmann II-V grades simply
by adding the appropriate amount of water to the Brockmann I
grade. Shake the material well to disintegrate lumps and allow it
to equilibrate in a closed vessel overnight.

Applications
● Neutral aluminacan be used for removing impurities from
natural alkaloids, vitamins, antibiotics, glycosides, and P000616

synthetic hormones, and for drying and purifying solvents.

● Basic aluminacan be used for removing peroxides from
ethers and hydrocarbons, extracting polar compounds Activated Alumina
(such as alcohols), drying solvents (diethylether, benzene,

O r d e r : 1 . 8 0 0 . 3 2 5 . 3 0 1 0 Te c h n i c a l S e r v i c e : 1 . 8 0 0 . 3 5 9 . 3 0 4 1 We b : w w w. s i g m a - a l d r i c h . c o m / s u p e l c o
chloroform), separating xylenes, or in dioxin analyses TYPE WEIGHT CAT. NO. PRICE
(EPA Method 1613) or pesticide analyses. Neutral 5g 199974-5G
100g 199974-100G
● Acidic aluminais useful for adsorbing polar compounds 1000g 199974-1KG
(such as vitamins), inorganic cations, water-soluble dyes, 5000g 199974-5KG
morphine, fatty acids, plant waxes, and in dioxin analyses
Basic 5g 199443-5G
(EPA Method 1613).
100g 199443-100G
1000g 199443-1KG
5000g 199443-5KG
TYPICAL PARTICLE SIZE DISTRIBUTION
FOR ACTIVATED ALUMINA (ALL GRADES) Acidic 100g 199966-100G
Sieve Mesh % 1000g 199966-1KG
Opening (mm) Size Retained 5000g 199966-5KG
0.25 60 <0.1 Weakly acidic 250g 267740-250G
0.20 ~75 2-5 1000g 267740-1KG
0.15 100 15-20 5000g 267740-5KG
0.10 ~150 55-65
0.07 ~210 72-85
0.04 ~360 95-98 Celite Filter Aid

Resins & Media

Reagent-grade Celite 545 AW. Acid-washed, high-purity flux-
AMOUNT OF WATER TO BE ADDED APPROX. WATER CONTENT
TO BROCKMANN I GRADE (%, KARL FISCHER METHOD)
calcined diatomaceous silica, especially prepared for chromatogra-
phy and other laboratory applications.
For grade II: 3% 4 - 4.5
For grade III: 6% 7 - 7.5 DESCRIPTION QTY. CAT. NO. PRICE
For grade IV: 10% 11 - 11.5
Celite 545 AW 454g 20199-U
For grade V: 15% 16 - 16.5

CHARACTERISTICS OF STANDARD GRADES OF HELPFUL HINTS

ACTIVATED ALUMINA (BROCKMANN NO. 1) For aluminas in solid phase extraction tubes refer to the Sample
Particle Size (mesh): ~150 Preparation section of this catalog.
Pore Diameter (Å): 58
Surface Area (m 2/g): 155
Water (%): ~1.5
Na 2O (%): 0.4
Fe2O3 (% max.): 0.02
SiO2 (% max.): 0.02
CAT. NO. GRADE TYPE pH1 Cl- (meq/g)
199974 507-C-I neutral 7.5±0.5 0.03
199443 5016-A-I basic 9.5±0.5 —
199966 504-C-I acidic 4.5±0.5 0.14
267740 506-C-I weakly acidic 6.0±0.5 0.06
1
5% stirred aqueous suspension.

SUPELCO
 432
Resins
Adsorption Media

Ambersorb: High Performance Separations Combigel XE-305

Ambersorb synthetic carbonaceous adsorbents can be used in a Underivatized polystyrene, mesh size 50-100 – our answer to
diverse range of liquid or vapor phase separation applications, Amberlite XE-305. Unique swelling properties; combinatorial
including: chemistry support.
● Wastewater treatment See our combinatorial chemistry reaction vessels, vacuum
● High value separations manifolds, and accessories in the Sample Preparation section.

● Vapor phase treatment of toxic air emissions DESCRIPTION CAT. NO. PRICE
COMBIGEL XE-305
● Catalysts/catalyst supports
50g 502537B
● Ultrapure water preparation
Ambersorb adsorbents are available from Supelco in research- Lipophilic Sephadex
scale quantities, up to 1000g. Lipophilic Sephadex LH-20is a hydroxypropylated derivative of
Unique physical structure makes Ambersorb resins ideal for bulk-Sephadex G-25. It is used in gel permeation, normal phase
scale separation applications: partition, and adsorption chromatography of, e.g., lipids, steroids,
fatty acids, hormones, and vitamins. It also is used for adsorption
● High macro- and mesoporosity allow
fast mass transferof a
chromatography.
compound to the micropores
O r d e r : 1 . 8 0 0 . 3 2 5 . 3 0 1 0 Te c h n i c a l S e r v i c e : 1 . 8 0 0 . 3 5 9 . 3 0 4 1 We b : w w w. s i g m a - a l d r i c h . c o m / s u p e l c o

● Unique pore size distribution and surface chemistry provideDry Bead Diameter: 25-100µm
Swelling Ratio: 1g swells to approx. 4mL (water, methanol)
excellent adsorption capacity
pH Range: 2-13
● Easy regeneration in situ Storage: room temp.
● Excellent physical integrityeliminates concern about
dusting or attrition DESCRIPTION CAT. NO. PRICE
LIPOPHILIC SEPHADEX
Ambersorb Carbonaceous Adsorbents
10g LH20100-10G
DESCRIPTION QTY. CAT. NO. PRICE 50g LH20100-50G
Ambersorb 563 100g 10430-U 100g LH20100-100G
Ambersorb 572 100g 10432-U 500g LH20100-500G

TABLE 1. AMBERSORB 563 ADSORBENT HAS HIGH CAPACITY

FOR DICHLOROMETHANE — UNDER DRY OR HUMID CONDITIONS
Kinetic Saturation Capacity
Preconditioned
Resins & Media

90% Relative 90% Relative

Dry Air Humidity Humidity
mg/g mg/mL mg/g mg/mL mg/g mg/mL
83 ± 18 48 ± 14 52 ± 5 30 ± 4 43 ± 4 27 ± 2

TABLE 2. TYPICAL PROPERTIES OF AMBERSORB CARBONACEOUS ADSORBENTS

Surface Porosity (mL/g) Bulk Water
2
Area Micropores Mesopores Macropores Density Ash Adsorption Mesh
Adsorbent Hydrophobicity 1 (m 2/g) <20Å 20-500Å >500Å (g/cc) (%) (g/g) Size
Ambersorb 563 high 550 0.23 0.14 0.23 0.53 <0.05 0.12 20-50
Ambersorb 572 low 1100 0.41 0.19 0.24 0.49 <0.05 0.31 20-50
1
Based on adsorption of trichloroethylene from water.
2
Isotherm at 94% relative humidity.

SUPELCO
 433
Resins
Adsorption Media

Florisil: Magnesium Silicate Adsorbents

Florisil adsorbents are used in preparative and analytical chromatography, and are available in pesticide-residue (PR)
dard
and stan
grades, in powdered and hard granular forms. All grades have a surface area 2of
/g, 300m
pH = 8.5 and have been activated.
It provides consistent results
PR grade 60/100 Florisil is specially tested for separating chlorinated pesticides, as described by Mills(1,2).
for column cleanup and separation of chlorinated pesticide residues, prior to gas or thin layer chromatography. Each batch
s the meet
performance characteristics describedChanges
in of Official Methods of Analysis
, JAOAC, Chapter 24, 208(h), Vol.49, p 233, 1966.
Packaged in glass containers.

Applications
● Isolation of steroids, sex hormones, and related compounds
● Isolation of antibiotics and alkaloids
● Lipid separation
● Vitamin assay
● Purification of pharmaceuticals
● Separation of nitrogen compounds from hydrocarbons

O r d e r : 1 . 8 0 0 . 3 2 5 . 3 0 1 0 Te c h n i c a l S e r v i c e : 1 . 8 0 0 . 3 5 9 . 3 0 4 1 We b : w w w. s i g m a - a l d r i c h . c o m / s u p e l c o
● Separation of aliphatic/aromatic mixtures
● Liquid partition chromatography
● Decolorization
● Catalysis
● Sample cleanup for PCBs and pesticides analysis
P000621

Sample Handling
The US Environmental Protection Agency (EPA) Contract Laboratory Program (CLP) method for monitoring pesticides in water,
sediment, and soil samples from hazardous waste sites specifies use of Florisil solid phase extraction (SPE) cartridges
ainlesswith
steelst
or Teflon frits for sample cleanup. This mandatory cleanup procedure significantly reduces matrix interferences caused by polar
compounds. Our ENVI-Florisil SPE tubes fulfill the pesticide recovery requirements of the EPA CLP pesticide method. A 6mL
Supelclean LC-Florisil SPE tube allows quantification of PCBs in transformer oil at concentrations of 5 to 500ppm. Also
le availab
are
ORBO-60 tubes, which meet NIOSH 5503 requirements for monitoring airborne PCBs. For more information, request T394028.

Florisil Adsorbents

Resins & Media

GRADE FORM MESH SIZE PARTICLE SIZE (µm) QTY. CAT. NO. PRICE
Standard Granular 16-30 595-1190 100g 343994-100G
500g 343994-500G
Standard Granular 30-60 250-595 250g 288691-250G
1000g 288691-1KG
PR Coarse powder 60-100 149-250 900mL 20280-U
Standard Coarse powder 60-100 149-250 250g 220744-250G
1000g 220744-1KG
Standard Fine powder 100-200 74-149 454g 20281
Standard Fine powder <200 <74 250g 288705-250G
1000g 288705-1KG

RELATED INFORMATION HELPFUL HINTS

Mills, P.A., J. Assoc. Off. Anal. Chem.,
42: 734 (1959). For Florisil adsorbent in solid phase extraction tubes refer to the
Mills, P.A., J. Assoc. Off. Anal. Chem.,
44: 171 (1961). Sampling Preparation section of this catalog.
References not available from Supelco.

SUPELCO
 434
Resins
Adsorption Media

Supelco offers a comprehensive range of silica gels, including Davisil and E. Merck products. These materials are luseful
types for
of al
low pressure, medium pressure, and flash column chromatography. They can be applied to the cleanup and purificationange of a wide r
of synthetic and natural compounds.
The Aldrich Catalog/Handbook (available on request) lists a wide selection of silica gels and silicic acids for non-chromatogra
phic
purposes.

Davisil Silica
Davisil silica gels are available in various particle and pore sizes for preparative column chromatography. They have
cessed
been to
pro
minimize or eliminate impurities such as minor metallic oxides, which can modify the surface nature and unpredictablyorption
alter ads
processes.
● Davisil 923 silica meets ASTM D-1319-70 specifications for hydrocarbon analysis.
Low metal oxides content minimizes olefin polymerization.
● Davisil 12 silica is recommended for ASTM Method D-2007 (rubber extender/processing oils).

PHYSICAL CHARACTERISTICS OF DAVISIL SILICA GELS TYPICAL ELEMENTAL ANALYSIS FOR DAVISIL SILICAS:
O r d e r : 1 . 8 0 0 . 3 2 5 . 3 0 1 0 Te c h n i c a l S e r v i c e : 1 . 8 0 0 . 3 5 9 . 3 0 4 1 We b : w w w. s i g m a - a l d r i c h . c o m / s u p e l c o

Pore Surface Area Pore Volume pH Na (as Na 2O): 600ppm

Diameter (Å) (m 2/g) (mL/g) (5% slurry) Fe (as Fe2O3): <100ppm
22 800 0.43 3.8 C: <100ppm
30 550 0.43 5.5 heavy metals: <5ppm
60 500 0.75 6.5
150 300 1.10 7.0
300 150-170 1.1-1.2 7.0
500 75-85 1.1-1.2 7.0

Davisil Silica Gels

GRADE MESH SIZE PARTICLE SIZE (µm) PORE SIZE (Å) QTY. CAT. NO. PRICE
12 28-200 75-650 22 250g 214396-250G
1kg 214396-1KG
5kg 214396-5KG
62 60-200 75-250 150 100g 243981-100G
500g 243981-500G
2.5kg 243981-2.5KG
633 200-425 35-75 60 100g 236772-100G
1kg 236772-1KG
Resins & Media

10kg 236772-10KG
634 100-200 75-150 60 100g 236780-100G
1kg 236780-1KG
10kg 236780-10KG
635 60-100 150-250 60 100g 236799-100G
1kg 236799-1KG
10kg 236799-10KG
636 35-60 250-500 60 100g 236802-100G
1kg 236802-1KG
643 200-425 35-70 150 100g 236810-100G
1kg 236810-1KG
644 100-200 75-150 150 100g 236829-100G
1kg 236829-1KG
645 60-100 150-250 150 100g 236837-100G
1kg 236837-1KG
646 35-60 250-500 150 100g 236845-100G
1kg 236845-1KG
653XWP 230-400 35-70 300 100g 13660
663XWP 230-400 35-70 500 100g 13662
923 100-200 75-150 30 250g 214477-250G
1kg 214477-1KG

SUPELCO
 435
Resins
Adsorption Media

E. Merck Silica Gel

Merck silica gel 40, silica gel 60,and silica gel 100 are widely used for column chromatography. They are very pure, containing less
than 0.02% iron and less than 0.02% chloride. Silica gel 60 (extra pure)contains less than 0.0005% each of Cd, Cu, Pb,–3,PO and Zn,
– –2
and <0.008% Cl, <0.002% Fe, <0.004% NO 3
, and <0.003% SO . Merck silica number 9385 is widely used for purification of organic
synthesis products by gas-pressurized liquid chromatography.
SURFACE PORE
E. MERCK PARTICLE AREA SIZE
DESCRIPTION NUMBER SIZE (µm) (m2/g) (Å) pH (±0.5) QTY. CAT. NO. PRICE
Silica gel 40 10180 63-200 750 40 5.5 100g 403563-100G
1kg 403563-1KG
5kg 403563-5KG
Silica gel 40 10181 200-500 750 40 5.5 100g 242179-100G
500g 242179-500G
2kg 242179-2KG
Silica gel 60 15111 15-40 550 60 7.0 50g S9258-50G
100g S9258-100G
1kg S9258-1KG
Silica gel 60 9385 40-63 550 60 7.0 100g 227196-100G

O r d e r : 1 . 8 0 0 . 3 2 5 . 3 0 1 0 Te c h n i c a l S e r v i c e : 1 . 8 0 0 . 3 5 9 . 3 0 4 1 We b : w w w. s i g m a - a l d r i c h . c o m / s u p e l c o
1kg 227196-1KG
5kg 227196-5KG
25kg 227196-25KG
Silica gel 60 7734 63-200 550 60 7.0 100g 391484-100G
1kg 391484-1KG
5kg 391484-5KG
Silica gel 60 7754 63-200 550 60 7.0 25g 403598-25G
(extra pure) 100g 403598-100G
500g 403598-500G
Silica gel 100 10184 63-200 300 100 7.0-7.5 100g 403601-100G
1kg 403601-1KG
5kg 403601-5KG

Modified Silica Gels

Chemically bonding organosilanes to silica dramatically alters the surface polarity, producing chromatographic media ation with separ
properties complementary to “bare” silica. Characteristics in the table below represent nominal values for base SUPELCOSIL
silicas. A
is a cleaned, sized, and activated irregular-particle silica gel for clean-up of synthetic organic reaction mixtures,alysis
dioxin(US
an EPA
Method 1613), and other extraction procedures. It also is suitable for analyses of amino acids, aromatic hydrocarbons, s, nucleic
peroxide
acids, and steroids, fatty acids and lipids.

Resins & Media

Sigma-Aldrich and Supelco Bonded Phase Silicas
PARTICLE SIZE SURFACE AREA PORE SIZE
DESCRIPTION (µm) (m 2 /g) (Å) QTY. CAT. NO. PRICE
3-Aminopropyl 40-63 550 60 10g 364258-10G
50g 364258-50G
3-Chloropropyl 40-63 550 60 10g 364266-10G
50g 364266-50G
Octyl 40-63 550 60 5g 385441-5G
25g 385441-25G
Octadecyl 40-63 550 60 25g 377635-25G
100g 377635-100G
SUPELCOSIL A 75-150 500 60 100g 13650-U
(100-200 mesh) 500g 13651-U
Supelprep ABZ+Plus1 12 190-210 110-130 100g 54350-U
1
A highly deactivated reversed phase material that possesses an embedded polar (amide) group.

SUPELCO
 436
Resins
Adsorption Media

Polymeric Adsorbent Resins — Summary of Characteristics

APPROX. MEAN
PORE TRUE WET SKELETAL SURFACE MEAN
CHEMICAL VOLUME DENSITY DENSITY AREA PORE MESH SWELLING IN
RESIN NATURE (mL/g) (g/mL) (g/mL) (m 2/g) DIAM. (Å) SIZE TOLUENE (%)
AMBERLITE/AMBERCHROM ADSORBENTS
XAD-2 polyaromatic 0.65 1.02 1.08 300 90 20-60 —
XAD-4 polyaromatic 0.98 1.02 1.08 725 40 20-60 —
XAD-7HP acrylic ester 1.14 1.05 1.24 450 90 20-60 —
XAD-16 polyaromatic 1.82 1.02 1.08 800 100 20-60 —
XAD-16HP polyaromatic 1.82 1.02 1.08 800 100 20-60 —
XAD-1180 polyaromatic 1.68 1.01 1.04 500 300 20-60 —
CG-71m 1 polymethacrylate 1.17 — 1.28 500 250 50-100µm —
CG-71c1 polymethacrylate 1.17 — 1.28 500 250 80-160µm —
CG-161m 1 polyaromatic 1.45 — 1.08 900 150 50-100µm —
CG-161c1 polyaromatic 1.45 — 1.08 900 150 80-160µm —
CG-300s1 polyaromatic 1.66 — 1.08 700 300 20-50µm —
CG-300m 1 polyaromatic 1.66 — 1.08 700 300 50-100µm —
CG-300c1 polyaromatic 1.66 — 1.08 700 300 80-160µm —
O r d e r : 1 . 8 0 0 . 3 2 5 . 3 0 1 0 Te c h n i c a l S e r v i c e : 1 . 8 0 0 . 3 5 9 . 3 0 4 1 We b : w w w. s i g m a - a l d r i c h . c o m / s u p e l c o

CG-1000s1 polyaromatic 1.66 — 1.08 250 1000 20-50µm —

DIAION/MCI GEL/SEPABEADS ADSORBENTS
Relite EXC04 sulfonated copolymer 0.60 — — 200 — 20-50 —
HP20 polyaromatic 1.30 1.01 — 500 260 20-60 30 4
HP20S polyaromatic 1.30 1.01 — 500 260 150-300µm 30 4
HP20SS polyaromatic 1.30 1.01 — 500 260 75-150µm 30 4
SP20SS polyaromatic 1.30 1.01 — 500 260 50-100µm 30 4
SP70 polyaromatic 1.10 1.01 — 700 65 250-850µm —
SP825L polyaromatic 1.40 1.01 — 1000 90-125 20-60 19 5
SP850 polyaromatic 1.20 1.01 — 1000 38 20-60 19 5
HP2MG polymethacrylate 1.20 1.09 — 500 170 25-50 5
SP207 brominated styrenic 1.10 1.18 — 650 105 20-60 18 6
CHP20P2 polyaromatic 1.30 1.01 — 500 400-600 75-150µm —
DOWEX ADSORBENTS
Styrene-DVB polyaromatic3 — — — — — 18-100 —
Styrene-DVB polyaromatic3 — — — — — 18-50 —
L-285 functionalized — — 1.04 800 25 20-50 —
L-323 polyaromatic 1.18 — 1.04 650 100 16-50 —
L-493 polyaromatic 1.16 — — 1100 46 20-50 —
V-493 polyaromatic 1.16 — — 1100 46 20-50 —
Resins & Media

V-502 polyaromatic 0.94 — 0.40 1080 34 1500µm —

SD-2 aminated copolymer — — — 800 50 18-50 —
DUOLITE ADSORBENTS
XAD-761 methylol 0.43 1.11 1.24 300 600 16-50 —
SUPELITE AND SUPELPAK ADSORBENTS
Supelite DAX-8 acrylic ester 0.79 1.09 1.23 160 225 40-60 —
Supelpak-2 polyaromatic 0.65 1.02 1.07 300 90 20-60 —
Supelpak-2B polyaromatic 0.65 1.02 1.07 300 90 20-60 —
1
Amberchrom resin (suspension in 20% ethanol in water)
2
MCI GEL resin
3
Physical parameters in this table do not apply to these nonfunctionalized copolymer materials.
4
In methanol 26%; in acetone 32%.
5
In methanol 16%; in acetone 15%; in isopropanol 17%; in butyl acetate 17%.
6
In methanol 15%; in acetone 15%; in isopropanol 17%; in butyl acetate 18%.

HELPFUL HINTS
Numerous Dow, Misubishi, and Rohm & Haas resins are available
in the Supelco warehouse but not listed here. If a resin you need
is not listed here, please contact your local Sigma-Aldrich
representative with your requirements.

SUPELCO
 437
Resins
Adsorption Media

Adsorbent Resins
DESCRIPTION QTY. CAT. NO. PRICE DESCRIPTION QTY. CAT. NO. PRICE
AMBERLITE RESINS AMBERCHROM RESINS(CONT’D)
Amberlite XAD-2 Amberchrom CG-1000
Polyaromatic Applications: Hydrophobic compounds up to 20,000 Polyaromatic Applications: Hydrophobic compounds; bulky
MW; phenol, organic removal; surfactants; aroma compounds; surfactants; large proteins; antibiotics.
catalyst; metals; antibiotic recovery. For clean US EPA versions see s 100mL 13911
Supelpak-2 and -2B. DIAION/SEPABEADS RESINS (MITSUBISHI)
100g 20275
500g 10357 HP20
Amberlite XAD-4 Polyaromatic Particle Size: 250-850µm Applications : Hydrophobic
Polyaromatic Applications: Small hydrophobic compounds; compounds; desalting; antibiotics; biomolecules. Broad application
surfactants; pharmaceutical manufacturing; phenol, chlorinated base.
organics, pesticide removal and recovery; organic removal from 100g 13605
aqueous food streams. 500g 13606
100g 20276 1000g 13607
500g 10358 HP20SS
Amberlite XAD-7HP Polyaromatic (small particle version of HP20) Particle Size: 75-150µm
High performance version of XAD-7 (no longer available) Applications: Hydrophobic compounds; biomolecules from fermenta-
tion broths; rapid kinetics for large molecules; nonaqueous applica-

O r d e r : 1 . 8 0 0 . 3 2 5 . 3 0 1 0 Te c h n i c a l S e r v i c e : 1 . 8 0 0 . 3 5 9 . 3 0 4 1 We b : w w w. s i g m a - a l d r i c h . c o m / s u p e l c o
Acrylic ester (moderate polarity) Applications: Compounds up to
60,000 MW; insulin recovery; fulvic and humic compounds; dry waste; tions; industrial fractionation of small biomolecules.
organic removal and recovery; antibiotic recovery. 100g 13613-U
100g 13361-U 1000g 13615-U
500g 13362-U SP20SS
Amberlite XAD-8 Polyaromatic. Particle Size: 50-100µm (fines removed) Applications :
See Supelite DAX-8. Small and medium proteins; hydrophobic compounds; chromato-
graphic separations of peptides, amino acids; reversed phase
Amberlite XAD-16
100g 13617-U
Polyaromatic Applications: Hydrophobic compounds up to 40,000
500g 13618-U
MW; antibiotics; pharmaceutical manufacturing; surfactants; bitters;
1000g 13619-U
separation of large organic molecules (especially proteins). More efficient
than XAD-2. SP70
100g 10347 Polyaromatic Particle Size: 250-850µm. Applications: debittering
500g 10345 juices and related food products; high capacity for naringen;
precleaned.
Amberlite XAD-16HP Meets requirements of FDA Food Additive Regulation 21 CFR 173.25.
Same as XAD-16, but specially cleaned to meet FDA 21 CFR 173.65 for 1000g 13962-U
removal of organic substances from aqueous foods, except carbonated
beverages. SP825L
Meets requirements of FDA Food Additive Regulation 21 CFR 173.25. Polyaromatic Particle Size: 250-600µm (fines removed)
100g 13355-U Applications: Antibiotics; organics; decolorization. High capacity for
small molecules.

Resins & Media

1000g 13357-U
1000g 13883
Amberlite XAD-1180
Polyaromatic (less polar than XAD-4) Applications: Hydrophobic SP850
compounds; gallium; myxovirescins; phosphoric acid; riboflavin salts; Polyaromatic Particle Size: 250-850µm Applications: Antibiotics;
antibiotic, vitamin, amino acid, and enzyme purification. organics; hydrophobic compounds; adsorbing large quantities of small
100g 10377 molecules.
500g 10378 100g 13597-U
1000g 13599
AMBERCHROM RESINS
HP2MG
Amberchrom CG-71 Polymethacrylic (intermediate polarity) Particle Size: 300-700µm.
Polymethacrylate (moderate polarity) Applications: Amino acid Applications: Hydrophobic compounds; antibiotics; aliphatics; color
separations (e.g., aspartame from phenylalanine); insulin recovery; bodies; broad spectrum adsorption of small and large molecules.
peptides; aromatics. 100g 13601
m 100mL 10367 1000g 13603
c 100mL 10366
SP207
Amberchrom CG-161 Brominated polyaromatic Particle size: 250-800µm
Polyaromatic Applications: Hydrophobic compounds; organics (e.g., Applications: Upflow fluidized bed applications; strongly hydrophobic,
phenol); surfactants; ethidium bromide; antibiotics; peptide and amino high density, large capacity; more hydrophobic than styrene/DVB
acid separations; small proteins. polymers.
m 100mL 10369 1000g 13623-U
c 100mL 10370-U
MCI GEL CHP20P
Amberchrom CG-300
Polyaromatic Particle Size: 75-150µm Applications:
Polyaromatic Applications: Hydrophobic compounds; surfactants;
Biopharmaceuticals; aromatic compounds; peptides; steroids;
medium-sized proteins; large peptides; antibiotics.
desalting; reversed phase applications. Good for nonaqueous use.
s 100mL 13908
100g 13629-U
m 100mL 13909-U
500g 13630-U
c 100mL 13910-U

SUPELCO
 438
Resins
Adsorption Media

DESCRIPTION QTY. CAT. NO. PRICE DESCRIPTION QTY. CAT. NO. PRICE
DOWEX RESINS DUOLITE RESINS
Styrene-DVB XAD-761 (previously known as S-761)
Polyaromatic Applications: hydrophobic compounds; filtering aids; Phenol-formaldehyde with methylol functionality (hydrophilic, highly
drilling muds; synthetic starting material. Mesh Size: 18-100 porous) Mesh Size: 16-50 Applications: Removal of proteins; removal
1000g 13390 of high MW colorants; organic impurities; pharmaceuticals.
Optipore L-285 (formerly XUS-40285) 500g 10356
Hydrophilic functionalized polyaromatic Applications: Surfactants; SUPELITE AND SUPELPAK RESINS
hydrophobic compounds; good for very hydrophobic proteins; Supelite DAX-8
decolorization. Acrylic ester (moderate polarity) Applications: compounds up to
1000g 13479-U 150,000 MW; treatment of paper pulp mill wastes; alcohols; surfac-
Optipore L-323 (formerly XUS-40323) tants; decolorization, fulvic and humic compounds; organic removal.
Polyaromatic (macroporous) Applications: Adsorption of soluble 100g 20278
organics from polar solvents; aliphatic alcohols, glycols, glycerine from
Supelpak-2 (a purified form of Amberlite XAD-2)
hydroxylic solvents; steroids; emulsifiers; surfactants; color compounds.
Polyaromatic Applications: Air sampling.
1000g 13475-U
Meets US EPA-recommended criteria for purity, as outlined in Level I
Optipore L-493 (formerly XUS-43493.00) Environmental Assessment Procedures Manual.
Polyaromatic (more hydrophobic than activated carbon) 100g 20279
Applications: Hydrophobic compounds; non-catalytic activity; liquid 1000g 21130-U
O r d e r : 1 . 8 0 0 . 3 2 5 . 3 0 1 0 Te c h n i c a l S e r v i c e : 1 . 8 0 0 . 3 5 9 . 3 0 4 1 We b : w w w. s i g m a - a l d r i c h . c o m / s u p e l c o

applications; phenol. Very high capacity for organics. Supelpak-2B (a purified form of Amberlite XAD-2)
100g 573698-100G Polyaromatic Applications: PCBs from water.
500g 573698-500G Specially cleaned to meet EPA requirements for determining PCBs in water
5kg 573698-5KG according to the Great Lakes National Program Office (GLNPO).
1 cubic foot 573698-1FT3 100g 13670
Optipore V-493 (dry) (formerly XUS-43493.01)
Polyaromatic (more hydrophobic than activated carbon)
Applications: Vapor applications; volatile organics and hazardous air
pollutants; non-catalytic activity.
100g 13485-U
500g 13486
1000g 13487
Optipore V-502 (dry) (formerly XU-43502.01)
Polyaromatic Applications: Vapor applications; volatile organics and
hazardous air pollutants. Low pressure drop. Large particle size version
of Optipore V-493.
1000g 13678
SD-2
Macroporous styrene divinylbenzene copolymer. Adsorbent. Ionic
Form: free base. Applications: Decolorization, taste and odor removal
Resins & Media

in sweetener applications.
Meets requirements of FDA Food Additive Regulation 21 CFR 173.25
100g 14043-U
1000g 14045-U

HELPFUL HINTS
Numerous Dow, Misubishi, and Rohm & Haas resins are available
in the Supelco warehouse but not listed here. If a resin you need
is not listed here, please contact your local Sigma-Aldrich
representative with your requirements.

SUPELCO
 439
Resins
HiTrap Cartridges

Amersham Pharmacia Biotech HiTrap Cartridges

● Affinity ● HIC
● Gel Filtration ● Ion Exchange

HiTrap Affinity Cartridges

HiTrap adapters and instruction manual included.
AFFINITY QTY. CAT. NO. PRICE
NHS-activated, 1mL 5 54830
NHS-activated, 5mL 1 54831
Blue, 1mL 5 54832
Blue, 5mL 1 54833
Chelating, 1mL 5 54834
Chelating, 5mL 1 54835
Heparin, 1mL 5 54836
Heparin, 5mL 1 54837
Protein A, 1mL 5 54838
Protein A, 5mL 1 54839

O r d e r : 1 . 8 0 0 . 3 2 5 . 3 0 1 0 Te c h n i c a l S e r v i c e : 1 . 8 0 0 . 3 5 9 . 3 0 4 1 We b : w w w. s i g m a - a l d r i c h . c o m / s u p e l c o
Protein G, 1mL 5 54840-U
Protein G, 5mL 1 54841
MAbTrap G II Kit1 54842
1
For fast, effective purification of monoclonal and polyclonal IgG from ascites
fluid, serum, or cell culture media, via syringe or pump. Sufficient materials
for 20 purifications. Contents: 1mL HiTrap protein G column, 50mL 10X
binding buffer concentrate (0.05% sodium azide preservative), 15mL 10X
elution buffer concentrate (0.05% sodium azide), 25mL neutralizing buffer
(0.05% sodium azide), luer adapter, domed nut, syringe, instructions.

HiTrap Gel Filtration (Sephadex G-25) Cartidges

HiTrap adapters and instruction manual included.
DESCRIPTION QTY. CAT. NO. PRICE
HiTrap Desalting Column 5 54822

HiTrap HIC Cartridges

Includes 1 each of five 1mL cartridges: Phenyl Sepharose High
Performance, Phenyl Sepharose 6 Fast Flow (low salt), Phenyl

Resins & Media

5
Sepharose 6 Fast Flow (high salt), Butyl Sepharose 4 Fast Flow, 44
00
Octyl Sepharose 4 Fast Flow, plus luer adapters, tubing connec- P0

tors, domed nuts, and instructions.

DESCRIPTION CAT. NO. PRICE HELPFUL HINTS
HiTrap HIC Test Kit 54814 Numerous Dow, Misubishi, and Rohm & Haas resins are available
in the Supelco warehouse but not listed here. If a resin you need
is not listed here, please contact your local Sigma-Aldrich
HiTrap Ion Exchange Cartridges
representative with your requirements.
Domed nuts, adapters, and instructions included.
DESCRIPTION QTY. CAT. NO. PRICE
HiTrap Q, 1mL 5 54815
HiTrap Q, 5mL 5 54816
HiTrap SP, 1mL 5 54817
HiTrap SP, 5mL 5 54818

RELATED INFORMATION
For additional information, request the following free literature by
phone or fax, or see our website.
No. Subject
T497080 HiTrap cartridges
T496127 HiTrap desalting columns
SUPELCO
 440
Resins
Affinity Media

Toyopearl Affinity Resins

These materials, consisting of 40-90µm hydrophilic polymer particles with 1000Å pores, can be used to rapidly separate
y a wide
or purif
variety of large biomolecules, such as enzymes and other proteins. Toyopearl resins feature:
● High resolving ability ● Wide pH range: 2-12
2
● Chemical resistance (0.5M NaOH or 0.5M HCl) ● Mechanical stability to 7kg/cm
(7 bar/100psi)

CHARACTERISTICS OF TOYOPEARL AFFINITY RESINS

Coupling Density
Resin Group (µmol/mL) Applications/Coupling Chemistries
REACTIVE RESINS
Formyl-650M aldehyde 60 couple to enzymes via primary amines; coupling agent: NaCNBH 4; optimal binding pH: 6.9-9.0
Amino-650M amine 90 couple ligands via carboxylate or aldehyde groups; coupling agent: carbodiimide or NaCNBH 4;
optimal binding pH: 4.5-6.0
ACTIVATED RESINS
Tresyl-650M tresyl 20 highly reactive to amine/thiol groups; no coupling agent required; optimal binding pH: 7-9
Epoxy-650M epoxide 800 immobilization of protein or low MW ligands (e.g., glutathione, glycine)
O r d e r : 1 . 8 0 0 . 3 2 5 . 3 0 1 0 Te c h n i c a l S e r v i c e : 1 . 8 0 0 . 3 5 9 . 3 0 4 1 We b : w w w. s i g m a - a l d r i c h . c o m / s u p e l c o

GROUP SPECIFIC RESINS

Chelate-650M iminodiacetic 35 for creating a metal bearing resin or IMAC (Ca +2, Ni +2, Zn +2, Co+2) to bind to histidine acid, free
cysteines of peptides or proteins, growth factors, tissue plasminogen activator, etc.
Heparin-650M heparin — purification of coagulation factors, lipoproteins, growth factors, enzymes active in nucleic acid
metabolism

Toyopearl Packings for Affinity Chromatography

TOYOPEARL PACKING QUANTITY CAT. NO. PRICE
Toyopearl AF-Chelate-650M 25mL 814475
Toyopearl AF-Heparin-650M 10mL 814473
Toyopearl AF-Tresyl-650M 5g 814471
Toyopearl AF-Amino-650M 25mL 808002
Toyopearl AF-Epoxy-650M 10g 808000
Toyopearl AF-Formyl-650M 25mL 808004
Resins & Media

HELPFUL HINTS
Numerous Dow, Misubishi, and Rohm & Haas resins are available
in the Supelco warehouse but not listed here. If a resin you need
is not listed here, please contact your local Sigma-Aldrich
representative with your requirements.

SUPELCO
 441
Resins
Gel Filtration Media

Sephacryl Sephadex (cont’d)

Crosslinked allyl dextran/N,N’ -methylenebisacrylamide QUANTITY CAT. NO. PRICE
copolymer for gel filtration. HR grades are smaller particles with G-25, 1K-5K (PROTEINS)1
narrower size distributions, for more efficient separations and
Fine
faster flow. Supplied swollen, suspended in 20% aqueous ethanol. 10g G2580-10G
Autoclavable, stable to 0.2M NaOH. Store at 0-5°C. 50g G2580-50G
100g G2580-100G
QUANTITY CAT. NO. PRICE 500g G2580-500G
S-100 HR, 1K-100K (PROTEINS)1
Superfine
100mL S100HR-100ML 10g G2550-10G
250mL S100HR-250ML 50g G2550-50G
750mL S100HR-750ML 100g G2550-100G
S-200 HR, 5K-250K (PROTEINS)1 G-50, 1.5K-30K (PROTEINS)1
100mL S200HR-100ML Coarse
250mL S200HR-250ML 10g G50300-10G
750mL S200HR-750ML 50g G50300-50G
S-300 HR, 10K-1500K (PROTEINS)1 100g G50300-100G
Medium

O r d e r : 1 . 8 0 0 . 3 2 5 . 3 0 1 0 Te c h n i c a l S e r v i c e : 1 . 8 0 0 . 3 5 9 . 3 0 4 1 We b : w w w. s i g m a - a l d r i c h . c o m / s u p e l c o
100mL S300HR-100ML
250mL S300HR-250ML 10g G50150-10G
750mL S300HR-750ML 50g G50150-50G
100g G50150-100G
S-400 HR, 20K-8000K (PROTEINS)1
500g G50150-500G
100mL S400HR-100ML
250mL S400HR-250ML Fine
750mL S400HR-750ML 10g G5080-10G
50g G5080-50G
S-500 HR, 40K-20,000K (DEXTRANS)1
100g G5080-100G
100mL S500HR-100ML 500g G5080-500G
250mL S500HR-250ML
750mL S500HR-750ML Superfine
10g G5050-10G
50g G5050-50G
100g G5050-100G
Sephadex
G-75, 3K-80K (PROTEINS)1
For purifying proteins and peptides by GFC with aqueous 10g G75120-10G
solvents. Dextran media with low exclusion limits are commonly 50g G75120-50G
used in rapid desalting procedures and in concentrating solutions 100g G75120-100G
of macromolecules. 500g G75120-500G

Resins & Media

Autoclavable, stable to 0.2M NaOH. Superfine
10g G7550-10G
QUANTITY CAT. NO. PRICE 50g G7550-50G
G-10, <700 1 100g G7550-100G
10g G10120-10G G-100, 4K-150K (PROTEINS)1
50g G10120-50G 10g G100120-10G
100g G10120-100G 50g G100120-50G
500g G10120-500G 100g G100120-100G
G-15, <1500 1 500g G100120-500G
10g G15120-10G Superfine
50g G15120-50G 10g G10050-10G
100g G15120-100G 50g G10050-50G
G-25, 1K-5K (PROTEINS)1 100g G10050-100G
Coarse
10g G25300-10G
50g G25300-50G Books on Gel Filtration
100g G25300-100G
500g G25300-500G DESCRIPTION CAT. NO. PRICE
Gel Filtration Chromatography 23578
Medium
10g G25150-10G
50g G25150-50G
100g G25150-100G RELATED INFORMATION
500g G25150-500G For a more complete discussion of our GFC media, request a copy of
Bulletin T194881.

1
Fractionation Range.
SUPELCO
 442
Resins
Gel Filtration Media

Sepharose Toyopearl Size Exclusion Packings 2

Beaded agarose for fractionating molecules of high molecular Toyopearl packings for size exclusion/gel filtration chromatogra-
weight. Crosslinked (CL) beaded agarose is more resistant to phy offer important features:
denaturing conditions and thus offers more versatility in the l High mechanical strength – use at high flow rates
choice of sample buffer and eluent. Supplied swollen.
l Stability in organic solvents
The approximate % agarose concentration is indicated by the
first digit of the catalog number, e.g.: “6” in 6B-100. l Can be packed by hydrostatic pressure or pump (5-7 bar)

Sepharose media should not be exposed to temperatures above 40°C. l pH range 2-12
Stable to 0.5M NaOH. Store at 0-5°C. l Can be autoclaved
Toyopearl media fractionate mixtures of proteins and other large
QUANTITY CAT. NO. PRICE
molecular weight compounds over a wide size range. You can use
6B, 10K-4000K (PROTEINS)1
these packings for such diverse analyses as separating RNA from
100mL 6B100-100ML protein, resolving oligosaccharides by degree of polymerization,
500mL 6B100-500ML
and isolating agglutinin while maintaining a high hemagglutina-
1liter 6B100-1L
tion titer. Toyopearl HW-40 gel, in particular, can be used very
CL-6B, 10K-4000K (PROTEINS)1
effectively with organic solvents.
100mL CL6B200-100ML
O r d e r : 1 . 8 0 0 . 3 2 5 . 3 0 1 0 Te c h n i c a l S e r v i c e : 1 . 8 0 0 . 3 5 9 . 3 0 4 1 We b : w w w. s i g m a - a l d r i c h . c o m / s u p e l c o

500mL CL6B200-500ML TOYOPEARL PARTICLE QUANTITY

1liter CL6B200-1L PACKING SIZE (µm) (mL) CAT. NO. PRICE
4B, 60K-20,000K (PROTEINS)1 HW-40C 50-100 500 807449
HW-40F 30-60 500 807448
100mL 4B200-100ML
HW-40S 20-40 250 807451
500mL 4B200-500ML
HW-50F 30-60 500 807453
1liter 4B200-1L
HW-50S 20-40 250 807455
CL-4B, 60K-20,000K (PROTEINS)1 HW-55F 30-60 500 807457
100mL CL4B200-100ML HW-55S 20-40 250 807459
500mL CL4B200-500ML HW-65F 30-60 500 807465
1liter CL4B200-1L HW-65S 20-40 250 807467
HW-75F 30-60 500 807469
2B, 70K-40,000K (PROTEINS)1
100mL 2B300-100ML
500mL 2B300-500ML
1liter 2B300-1L Toyopearl Size Exclusion LABPAK Sample Kits
CL-2B70K-40,000K (PROTEINS)1 LABPAK samplers enable you to try several Toyopearl resins to
100mL CL2B300-100ML determine which works best for your particular application.
500mL CL2B300-500ML
PARTICLE MW RANGE
1liter CL2B300-1L
SIZE (GLOBULAR
Resins & Media

Superdex RESIN (µm) PROTEINS)2 CAT. NO. PRICE

A composite matrix of dextran and highly crosslinked porous SECPAK LMW Sampler 843300
(150mL each resin)
agarose, Superdex media combine the high selectivity of a HW-40F 30-60 100-10,000
Sephadex matrix with the chemical and physical stability of HW-50F 30-60 500-80,000
crosslinked agarose. Optimized for high resolution preparative HW-55F 30-60 1-700 x 10 3
separations. Supplied in 20% ethanol. Also available as a 13µm SECPAK HMW Sampler 843310
(150mL each resin)
bead, packed in FPLC columns (see the Biopolymer Columns HW-55F 30-60 1-700 x 10 3
section). HW-65F 30-60 40-5000 x 10 3
HW-75F 30-60 500-50,000 x 10 3
Store at 0-5°C.
SECPAK HP Sampler 843350
QUANTITY CAT. NO. PRICE (120mL each resin)
1 HW-40S 20-40 100-10,000
SUPERDEX 30 PREP GRADE, <10K (PROTEINS) HW-50S 20-40 500-80,000
25mL S2792-25ML HW-55S 20-40 1-700 x 10 3
HW-65S 20-40 40-5000 x 10 3
100mL S2792-100ML
1
SUPERDEX 75 PREP GRADE, 3K-70K (PROTEINS)
25mL S6657-25ML
HELPFUL HINTS
100mL S6657-100ML
1
SUPERDEX 200 PREP GRADE, 10K-600K (PROTEINS)
Numerous Dow, Misubishi, and Rohm & Haas resins are available
in the Supelco warehouse but not listed here. If a resin you need
100mL S6782-100ML
is not listed here, please contact your local Sigma-Aldrich
representative with your requirements.

1
Fractionation Range.
2
Ranges for dextrans: HW-40: 100-7000; HW-50: 500-20,000; HW-55: 1000-200,000; HW-65: 10,000-1,000,000; HW-75: 100,000-10,000,00 0.
SUPELCO
 443
Resins
Hydrophobic Interaction Media

Sepharose Hydrophobic Interaction Media Toyopearl Hydrophobic Interaction Media

Long-term pH stability: 3-13 (3-12 for Octyl Sepharose CL-4B Toyopearl HIC media offer these advantages:
and Phenyl Sepharose CL-4B). ● Strong affinity for water-soluble proteins
LIGAND ● High recovery of mass and activity
MEAN DENSITY
PARTICLE (µmol/mL ● High sample capacity – up to 2-4 times
SIZE (µm) DRY GEL) QTY. CAT. NO. PRICE that of conventional gels
Phenyl Sepharose High Performance (6% crosslinked agarose) ● Fluctuating salt concentrations will
34 25 10mL P2209-10ML
not change bed volume
50mL P2209-50ML
2
● Mechanical stability to 7kg/cm
(7 bar/100psi)
Phenyl Sepharose 6 Fast Flow, low sub (6% crosslinked agarose)
45-165 20 50mL P2334-50ML ● pH range 2-12
200mL P2334-200ML
● Clean in place with 0.5M NaOH
Phenyl Sepharose 6 Fast Flow, high sub (6% crosslinked agarose)
● Autoclavable
45-165 40 50mL P2459-50ML
200mL P2459-200ML Toyopearl media offer high yield recovery of proteins, using
various aqueous eluants. The resins have an exclusion limit of 5 x
Butyl Sepharose 4 Fast Flow (4% crosslinked agarose)

O r d e r : 1 . 8 0 0 . 3 2 5 . 3 0 1 0 Te c h n i c a l S e r v i c e : 1 . 8 0 0 . 3 5 9 . 3 0 4 1 We b : w w w. s i g m a - a l d r i c h . c o m / s u p e l c o
10mL B9041-10ML 106 Dalton. The large pore size, 1000Å, enables these packings to
45-165 50 50mL B9041-50ML separate very large proteins by a hydrophobic interaction
mechanism, without size exclusion effects.
Octyl Sepharose 4 Fast Flow (4% crosslinked agarose)
10mL O0511-10ML 100mL bottle
45-165 5 200mL O0511-200ML
TOYOPEARL PARTICLE
Octyl Sepharose CL-4B (4% crosslinked agarose) MEDIUM SIZE (µm) CAT. NO. PRICE
45-165 40 50mL O6001-50ML Butyl-650C 60-150 807478
200mL O6001-200ML Butyl-650M 40-90 807477
Butyl-650S 20-50 807476
Phenyl Sepharose CL-4B (4% crosslinked agarose)
Phenyl-650M 40-90 814478
10mL P7892-10ML
Phenyl-650S 20-50 814477
45-165 40 50mL P7892-50ML
Ether-650M 40-90 816173
200mL P7892-200ML

HELPFUL HINTS Toyopearl Hydrophobic Interaction LABPAK Sampler Kits

Numerous Dow, Misubishi, and Rohm & Haas resins are available LABPAK kits enable you to try several Toyopearl resins to
in the Supelco warehouse but not listed here. If a resin you need determine which works best for your particular application.
is not listed here, please contact your local Sigma-Aldrich

Resins & Media

PARTICLE
representative with your requirements.
SIZE
KIT (µm) HYDROPHOBICITY CAT. NO. PRICE
HICPAK SAMPLER
50mL each resin 843100
Ether-650M 40-90 weak
Phenyl-650M 40-90 medium
Butyl-650M 40-90 strong
HICPAK HP SAMPLER
25mL each resin 843150
Ether-650S 20-50 weak
Phenyl-650S 20-50 medium
Butyl-650S 20-50 strong

SUPELCO
 444
Resins
Anion Exchange Media

Strong Anion Exchangers on Polystyrene (Type I and Type II)

CROSS MAX. OP. TOTAL
LINK IONIC MOISTURE TEMP. EXCHANGE CAPACITY pH
EXCHANGER (%) MATRIX1 MESH FORM (Approx. %) (°C) 2 meq/mL meq/g RANGE
AMBERLITE STRONG ANION EXCHANGERS, TYPE I (QUATERNARY AMMONIUM)
IRA-900 Cl — Mr 16-50 Cl 60 60/77 1.0 4.2 0-14
IRA-958 Cl — Mr 16-50 Cl 69 38/77 0.80 4.1 0-14
IRA-400 Cl 8 G 16-50 Cl 45 60/77 1.4 3.8 0-14
IRA-400 OH 8 G 16-50 OH 45 60/77 1.2 4.0 0-14
IRA-402 Cl 6 G 16-50 Cl 53 60/77 1.2 4.1 0-14
IRA-458 Cl — G 16-50 Cl 58 38/60 1.2 4.4 0-14
4200 (Cl) 3 — G 625µm Cl 53 50 1.2 3.7 0-14
IRN78 8 G 16-50 OH ≤60 60 1.1 4.0 0-14
A26 — MR 16-45 OH 69 60/90 0.95 4.2 0-14
DIAION STRONG ANION EXCHANGERS, TYPE I (QUATERNARY ALKYLAMINE)
HPA25 — HP 30-70 Cl 63 70/90 0.6 2.4 0-14
NSA100 — G 16-50 Cl 41 60/80 1.3 3.4 0-14
PA306S 3 P 40-100 Cl 71 60/80 0.8 4.2 0-14
PA308 4 P 16-50 Cl 62 60/77 1.0 4.0 0-14
O r d e r : 1 . 8 0 0 . 3 2 5 . 3 0 1 0 Te c h n i c a l S e r v i c e : 1 . 8 0 0 . 3 5 9 . 3 0 4 1 We b : w w w. s i g m a - a l d r i c h . c o m / s u p e l c o

PA312 6 P 16-50 Cl 52 60/77 1.2 3.7 0-14

PA316 8 P 16-50 Cl 47 60/80 1.3 — 0-14
SA10A — G 16-50 Cl 45 60/77 1.3 3.4 0-14
SA11A — G 16-50 Cl 50 60/77 0.8 2.6 0-14
SA12A — G 16-50 Cl 52 60/80 1.3 — 0-14
DOWEX STRONG ANION EXCHANGERS, TYPE I (TRIMETHYLBENZYL AMMONIUM)
1x2 2 G 16-100 Cl 75 66/99 0.7 4.2 0-14
1x2 2 G 50-100 Cl 70 66/99 0.7 3.5 0-14
1x2 2 G 100-200 Cl 75 66/99 0.6 3.5 0-14
1x2 2 G 200-400 Cl 75 66/99 0.6 3.5 0-14
1x4 4 G 20-50 Cl ≥50 66/99 1.0 3.5 0-14
1x4 4 G 50-100 Cl ≥50 66/99 1.0 3.5 0-14
1x4 4 G 100-200 Cl 59 66/99 1.0 3.5 0-14
1x4 4 G 200-400 Cl 59 66/99 1.0 3.5 0-14
1x8 8 G 50-100 Cl 46 66/99 1.2 3.5 0-14
1x8 8 G 100-200 Cl 42 66/99 1.2 3.5 0-14
1x8 8 G 200-400 Cl 42 66/99 1.2 3.5 0-14
11 4 — G 640µm Cl 52 60 1.2 3.6 0-14
550A5 — G 550µm OH 47(Cl) 60 1.2 3.4 0-14
550A UPW5 — G 550µm OH 47(Cl) 60 1.2 3.4 0-14
MSA4 — Mp 640µm Cl 60 60/99 1.0 4.0 0-14
SBR-C 8 G 16-45 Cl 46 66/99 1.4 3.7 0-14
Resins & Media

SBR LC NG 8 G 16-50 OH ≤60 60 1.1 – 0-14

SBR-P-C 8 G 16-45 OH 54 66/99 1.2 4.0 0-14
21K — G 16-30 Cl 54 60/100 1.2 3.8 0-14
A4 — G 610µm OH 66 60/100 1.0 — 0-14
A4 — G 575µm Cl 57 60 1.2 4.0 0-14
21K XLT — G 16-20 Cl 55 60/100 1.2 3.8 0-14
AMBERLITE STRONG ANION EXCHANGERS, TYPE II (DIMETHYLETHANOLAMINE)
IRA-910 Cl — Mr 16-50 Cl 52 40/77 1.0 3.8 0-14
IRA-410 Cl — G 20-50 Cl 42 40/77 1.4 3.4 0-14
DIAION STRONG ANION EXCHANGERS, TYPE II (QUATERNARY ALKYLALKANOLAMINE)
PA408 4 P 16-50 Cl 59 40/60 0.9 3.3 0-14
PA412 6 P 16-50 Cl 49 40/60 1.1 — 0-14
PA418 9 P 16-50 Cl 41 40/60 1.3 3.3 0-14
SA20A — G 16-50 Cl 42 40/60 1.3 3.2 0-14
SA21A low G 16-50 Cl 60 40/60 0.8 3.1 0-14
DOWEX STRONG ANION EXCHANGERS, TYPE II (DIMETHYLETHANOLBENZYL AMMONIUM)
2x8 8 G 50-100 Cl <38 66/99 1.2 — 0-14
2x8 8 G 100-200 Cl 37 66/99 1.2 — 0-14
2x8 8 G 200-400 Cl 37 66/99 1.2 — 0-14
22 — Mp 16-50 Cl 52 46 1.2 — 0-14
A24 — G 550µm Cl 42 35/79 1.3 3.2 0-14
1
G = gel; Mr = macroreticular; Mp = macroporous; HP = highly porous; P = porous
2
OH/Cl form
3
Amberjet exchanger
4
Marathon exchanger
5
Monosphere exchanger

SUPELCO
 445
Resins
Anion Exchange Media

Weak Anion Exchangers on Polystyrene

CROSS MAX. OP. TOTAL
LINK IONIC MOISTURE TEMP. EXCHANGE CAPACITY pH
EXCHANGER (%) MATRIX1 MESH FORM (Approx. %) (°C) 2 meq/mL meq/g RANGE
AMBERLITE WEAK ANION EXCHANGERS (POLYAMINE)
IRA-67 — G 16-50 FB3 60 60 1.6 5.6 0-7
IRA-95 — Mr 16-50 FB58 100 1.3 4.7 0-7
IRA-96 — Mr 16-50 FB60 100 1.6 5.6 0-7
IRA-743 — — 16-50 FB58 100 0.6 — 0-10
DIAION WEAK ANION EXCHANGERS (ALKYLAMINE)
WA10 — G 16-50 FB66 60 1.2 — 0-9
WA21J — HP 16-50 FB46 100 2.0 5.8 0-9
WA30 — HP 16-50 FB49 100 1.5 3.0 0-9
CRB02 — HP 16-50 ≤55
FB 60/79 0.8 2.7 0-14
DOWEX WEAK ANION EXCHANGERS (POLYAMINE)
66 — Mp 16-50 FB45 60 1.4 4.0 0-7
66 — Mp 550µm FB45 60 1.4 4.0 0-7
77 — Mp 550µm FB45 60 1.7 4.8 0-7

O r d e r : 1 . 8 0 0 . 3 2 5 . 3 0 1 0 Te c h n i c a l S e r v i c e : 1 . 8 0 0 . 3 5 9 . 3 0 4 1 We b : w w w. s i g m a - a l d r i c h . c o m / s u p e l c o
WBA4 — Mp 400µm FB54 60/93 5 1.25 4.2 0-7
WBA-24 — Mp 550µm FB45 60 1.7 4.8 0-7
M-43 — Mp 16-50 FB ≤45 60 1.55 — 0-14
DUOLITE WEAK ANION EXCHANGERS (POLYAMINE)
A-7 — Mr 16-50 FB56 40 2.2 13.9 0-6
1
G = gel; Mr = macroreticular; Mp = macroporous; HP = highly porous; P = porous
2
OH/Cl form
3
free base
4
Marathon exchanger
5
FB/HCl

Comparable Anion Exchangers on Polystyrene

AMBERLITE DIAION DOWEX HELPFUL HINTS
STRONG TYPE I Numerous Dow, Misubishi, and Rohm & Haas resins are available
IRA-900 PA308/PA312 11/MSA in the Supelco warehouse but not listed here. If a resin you need
IRA-9046 PA308 11/MSA is not listed here, please contact your local Sigma-Aldrich
IRA-9386 representative with your requirements.
IRA-958

Resins & Media

6
IRA-400 SA10A SBR
6
IRA-400(OH) SA10A SBR
IRA-401S6 SA11A 1x4
IRA-402, IRA-4046 SA12A 11/SBR-P6

IRA-420C6 SA10A 11/21K/SBR6/SBR-P6

IRA-458
IRA-420C6 SA10AS6
Amberjet 4200 550A, G-55 6, N-196 6
STRONG TYPE II
IRA-910 PA418 MSA-26, 22
IRA-410/4166 SA20A6 SAR6, A2
WEAK
IRA-677 WA1036
IRA-93, IRA-95 WA30 66/MWA-16
IRA-946, IRA-96 WA30 N-2836
6
Not available. Substitute WA10.
7
Formerly IRA-68.

SUPELCO
 446
Resins
Anion Exchange Media

Amberlite Strong Anion Exchangers, Type I Diaion Strong Anion Exchangers, Type I
Functional Group: quaternary ammonium Functional Group: quaternary alkylamine
Effective pH Range:0-14. Effective pH Range:0-14.
Meet requirements of FDA Food Additive Regulation 21 CFR Meet requirements of FDA Food Additive Regulation 21 CFR
173.25. 173.25.
QTY. CAT. NO. PRICE QTY. CAT. NO. PRICE
IRA900 HPA25
Polystyrene, macroreticular Form: chloride Applications: decolorizing Polystyrene, highly porous Form: chloride Applications: large organics;
intermediate to light solutions; heparin at neutral pH; metal ions; hydrogen enzyme immobilization; demineralization; deashing
peroxide (highest porosity Type I strong anion exchanger available)
500g 10381-U 1000g 13491-U
IRA958 PA308
Polystyrene, macroreticular Form: chloride Applications: removal of color Polystyrene, porous Crosslinkage: 4% Form: chloride Applications:
and organics; cane sugar treatment; metal complexes; citric acid (resistant deionization of water; catalyst; separation of amino acids; recovery of
to organic fouling) metals; sugar refining; decolorization; formalin refining
500g 10337 1000g 13495
IRA400 PA312
Polystyrene, gel Crosslinkage: 8% Form: chloride Applications: usually Polystyrene, porous Crosslinkage: 6% Form: chloride Applications:
used for treatment of waters that are essentially free of organic material; deioniz-ation of water; catalyst; separation of amino acids; recovery of
deionization including silica reduction; deoxygenating; removal of amino metals; sugar refining; decolorization; formalin refining
acids at high pH; separation of kanamycin A&B 1000g 13499
O r d e r : 1 . 8 0 0 . 3 2 5 . 3 0 1 0 Te c h n i c a l S e r v i c e : 1 . 8 0 0 . 3 5 9 . 3 0 4 1 We b : w w w. s i g m a - a l d r i c h . c o m / s u p e l c o

500g 10326
IRA400 Dowex Strong Anion Exchangers, Type I
Polystyrene, gel Crosslinkage: 8% Form: hydroxide Applications: same as
for IRA400Cl Functional Group: trimethylbenzyl ammonium
500g 501980
Effective pH Range:0-14
IRA402
Polystyrene, gel Crosslinkage: 6% Form: chloride Applications: water
Meet requirements of FDA Food Additive Regulation 21 CFR 173.25
conditioning; removal of weakly acidic contaminants (chemically the same (unless otherwise noted).
as IRA-400, but with lower crosslinkage to give better diffusion rates with
large organics) QTY. CAT. NO. PRICE
500g 10328 1X2
IRA458 Polystyrene, gel Crosslinkage: 2% Form: chloride Applications: food
Polystyrene, gel Form: chloride Applications: treatment of water which
produces severe fouling with other resins; metals and metal salts/
& drug processing
complexes; biuret; (hydrophilic structure is more resistant to organic Manufactured under cGMP
fouling) Mesh Size: 16-100
500g 10330 1000g 13367
IRN78 Mesh Size: 50-100
Polystyrene, gel, Nuclear Grade Form: hydroxide, minimum of 95% 100g 44290-100G
exchange sites Applications: Water treatment, RAD waste, decontamina- 500g 44290-500G
tion, useful for boron thermal regeneration. 2500g 44290-2.5KG
500g 10343 1 cubic foot 44290-1FT3
Mesh Size: 100-200
Resins & Media

100g 217387-100G
Amberlite Strong Anion Exchangers, Type II 500g 217387-500G
2500g 217387-2.5KG
Functional Group: dimethylethanolamine 1 cubic foot 217387-1FT3
Effective pH Range:0-14. Mesh Size: 200-400
100g 217395-100G
Meet requirements of FDA Food Additive Regulation 21 CFR 500g 217395-500G
173.25. 2500g 217395-2.5KG
1 cubic foot 217395-1FT3
QTY. CAT. NO. PRICE 1X4
IRA410
Polystyrene, gel Form: chloride Applications: higher regeneration Polystyrene, gel Crosslinkage: 4% Form: chloride
efficiency; water conditioning; iodine ions; neutralization of solutions;Mesh Size: 20-50
xanthan and xanthene dyes 100g 428612-100G
500g 10329 500g 428612-500G
1 cubic foot 428612-1FT3
IRA910 Mesh Size: 50-100
Polystyrene, macroreticular Form: chloride Applications: improved 100g 44310-100G
regeneration efficiency; water conditioning and deionization; removal of 500g 44310-500G
sulfuric acid and color 1 cubic foot 44310-1FT3
500g 10334-U Mesh Size: 100-200
100g 428590-100G
500g 428590-500G
1 cubic foot 428590-1FT3
Mesh Size: 200-400
HELPFUL HINTS 100g 428604-100G
500g 428604-500G
Numerous Dow, Misubishi, and Rohm & Haas resins are available1 cubic foot 428604-1FT3
in the Supelco warehouse but not listed here. If a resin you need
is not listed here, please contact your local Sigma-Aldrich
representative with your requirements.

SUPELCO
 447
Resins
Anion Exchange Media

Dowex Strong Anion Exchangers, Type I (cont’d) Dowex Strong Anion Exchangers, Type II
QTY. CAT. NO. PRICE Functional Group: dimethylethanolbenzyl ammonium
1X8
Effective pH Range:0-14
Meet requirements of FDA Food Additive Regulation 21 CFR 173.25
Polystyrene, gel Crosslinkage: 8% Form: chloride
Mesh Size: 50-100 QTY. CAT. NO. PRICE
100g 217417-100G
2X8
500g 217417-500G
2500g 217417-2.5KG Styrene-divinylbenzene, gel Crosslinkage: 8% Form: chloride
1 cubic foot 217417-1FT3 Mesh Size: 50-100
Mesh Size: 100-200 100g 428620-100G
100g 217425-100G 500g 428620-500G
500g 217425-500G 1 cubic foot 428620-1FT3
2500g 217425-2.5KG Mesh Size: 100-200
1 cubic foot 217425-1FT3 100g 428639-100G
Mesh Size: 200-400 500g 428639-500G
100g 44340-100G 2500g 428639-2.5KG
500g 44340-500G 1 cubic foot 428639-1FT3
2500g 44340-2.5KG Mesh Size: 200-400
1 cubic foot 44340-1FT3 100g 428647-100G
Marathon 11 (previously known as Dowex 11) 500g 428647-500G
Polystyrene, gel Form: chloride Applications: specifically suited for 5000g 428647-5KG
demineralization of high organic waters; removal of organic material and 1 cubic foot 428647-1FT3

O r d e r : 1 . 8 0 0 . 3 2 5 . 3 0 1 0 Te c h n i c a l S e r v i c e : 1 . 8 0 0 . 3 5 9 . 3 0 4 1 We b : w w w. s i g m a - a l d r i c h . c o m / s u p e l c o
color bodies; low silica leakage 22
1000g 13435 Styrene-divinylbenzene, macroporous Form: chloride Applications:
Monosphere 550A deashing and mixed bed polishing of high fructose corn syrups
Polystyrene, gel, Monodispersed Form: hydroxide Particle Size: 550µm 250g 436623-250G
1000g 436623-1KG
Applications: high efficiency and capacity for mixed-bed applications;
condensate polishing Marathon A2
250g 436607-250G Styrene-divinylbenzene, gel, monodispersed Form: chloride Particle Size:
1000g 436607-1KG 550µm Applications: demineralization; well suited for water with high
1 cubic foot 436607-1FT3 concentration of mineral acids (chlorides, sulfates) and low concentration of
silica and CO2 (<25%)
Marathon MSA (previously known as MSA-1) 250g 433934-250G
Polystyrene, macroporous Form: chloride Applications: removal of 1000g 433934-1KG
organic impurities with minimum organic fouling; deionization; silica
removal; condensate polishing
500g MSA1-500G Amberlite Weak Anion Exchangers
SBR-C Functional Group: polyamine
Polystyrene, gel Form: chloride Applications: narrower particle size
Meet requirements of FDA Food Additive Regulation 21 CFR 173.25
distribution suited to deep-bed condensate polishing; good for mixed-bed
applications; demineralization (unless otherwise noted).
100g 573701-100G
500g 573701-500G QTY. CAT. NO. PRICE
5000g 573701-5KG [^573701-5KG] IRA67 (also known as IRA-68)
1 cubic foot 573701-1FT3 Acrylic, gel Form: free base Applications: unusually high capacity for large
organics; deacidification, deionization of process liquors; isolation of acidic

Resins & Media

SBR LC NG
Polystyrene, gel, Nuclear Grade Form: hydroxide, minimum of 95% natural products; novabiacin and cephalosporins; separation of neutral and
acidic amino acids below pH 10; removal of heparinic acid at low pH; citric
exchange sites Applications: Water treatment, CVCS and radwaste
acid; demineralization of cheese whey
demineralizers, reactor coolant treatment; useful for boric acid recovery
100g 14035-U 500g 10331
1000g 14036-U IRA95
2500g 14037-U Styrenic, macroreticular Form: free baseApplications: exceptional
5000g 14038-U resistance to organic fouling; deacidification, deionization of water where
Dowex 21K removal of strong mineral and organic acids is desired; deionization of
Polystyrene, gel Form: chloride Applications: mining grade material process liquors; removal of heparinic acid at low pH; heavy metals
1000g 10425
Does not meet FDA 21 CFR 173.25
250g 436658-250G IRA96
Styrenic, macroreticular Form: free baseApplications: deionization;
Marathon A chromate recovery; formaldehyde deacidification; ammonium nitrate
Polystyrene, macroporous, monodispersed Form: chloride Particle Size: removal and recovery
575µm Applications: demineralization; water with high concentration of Does not meet FDA Food Additive Regulation 21 CFR 173.25.
weak ions (silica, CO2); organics-laden feed waters 100g A8709-100G
250g 433942-250G 500g A8709-500G
1000g 433942-1KG 1000g A8709-1KG
IRA743
Styrenic, macroreticular Form: free baseApplications: borate-specific weak
anion exchanger
100g IRA743-100G
HELPFUL HINTS 250g 216445-250G
Numerous Dow, Misubishi, and Rohm & Haas resins are available 500g IRA743-500G
1000g 216445-1KG
in the Supelco warehouse but not listed here. If a resin you need
is not listed here, please contact your local Sigma-Aldrich
representative with your requirements.

SUPELCO
 448
Resins
Anion Exchange Media

Diaion Weak Anion Echangers Dowex Weak Anion Exchangers

QTY. CAT. NO. PRICE Functional Group: polyamine
WA10 Meet requirements of FDA Food Additive
Regulation 21 CFR 173.25.
Acrylic, gel Functional Group: tertiary amine Form: free base
Applications: pretreatment of starch hydrolysates containing high levels of QTY. CAT. NO. PRICE
minerals; treatment of fluids containing troublesome foulants; purification
of dextrose, beet sugar solutions, or formaldehyde 66
1000g 13944-U Styrenic, macroporous Form: free base Applications: deashing and mixed
bed polishing of high fructose corn syrups
WA21J 250g 436674-250G
Styrenic, highly porous Functional Group: Primary and Secondary Amine 1000g 436674-1KG
Form: free base Reverse Swelling: (OH – → Cl–) 30% Applications: removal
of strong mineral acids from water; treatment of organic solvents; rigorous Monosphere 66
industrial applications. Styrenic, macroporous, monodispersed Form: free base Particle Size:
Meets requirements of FDA Food Additive Regulation 21 CFR 173.25. 550µm Applications: same as for Dowex 66.
1000g 13705
1000g 13895 Monosphere 77
WA30 Styrenic, macroporous, monodispersed Form: free base Particle Size:
Styrenic, highly porous Functional Group: alkylamine Form: free base 550µm Applications: deashing and mixed bed polishing of high fructose
Reverse Swelling: (OH – → Cl–) 30% Applications: best in class for high corn syrups
MW organic acids; strong decolorization capability; water treatment; 1000g 502529D
pretreatment of corn syrup, beet sugar and dextrose; refining of formalin, Marathon WBA (Replacement for MWA1)
glycerine, and enzymes; catalyst Styrenic, macroporous, monodispersed Form: free base Particle Size:
Meets requirements of FDA Food Additive Regulation 21 CFR 173.25. 400µm Applications: demineralization; well suited for combined use with
O r d e r : 1 . 8 0 0 . 3 2 5 . 3 0 1 0 Te c h n i c a l S e r v i c e : 1 . 8 0 0 . 3 5 9 . 3 0 4 1 We b : w w w. s i g m a - a l d r i c h . c o m / s u p e l c o

100g 13541 strong base anion resins for water with high concentration of mineral
1000g 13543 anions or high organic fouling potential
250g 436666-250G
CRB02 1000g 436666-1KG
Styrenic, highly porous Functional Group: glucamine Form: free base
Applications: high selectivity for borate ion; useful for low levels at high Dowex M-43
flow rates Styrenic, macroporous Functional Group: dimethylamine Form: free base
1000g 13959-U Applications: acts like an acid absorber, capable of removing both mineral
and organic acids; nothing released back into solution that will make a
sludge or change the process stream.
100g 14031-U
1000g 14032-U
2500g 14033-U
5000g 14034-U

Duolite Weak Anion Exchanger

HELPFUL HINTS QTY. CAT. NO. PRICE
Numerous Dow, Misubishi, and Rohm & Haas resins are available A-7
Phenol-formaldehyde, macroreticular Functional Group: polyamine Form:
in the Supelco warehouse but not listed here. If a resin you need free base Reverse Swelling: (OH– → Cl–) 23-28% Applications: sugar
is not listed here, please contact your local Sigma-Aldrich deionization; removal of high molecular weight colorants (e.g., wine
Resins & Media

representative with your requirements. processing); organic acids; lactose; metal ions (high porosity, hydrophilic)
500g 10348

SUPELCO
 449
Resins
Cation Exchange Media

Strong Cation Exchangers on Polystyrene

CROSS MAX. OP. TOTAL
LINK IONIC MOISTURE TEMP. EXCHANGE CAPACITY pH
EXCHANGER (%) MATRIX1 MESH FORM (Approx. %) (°C) 2 meq/mL meq/g RANGE
AMBERLITE STRONG CATION EXCHANGERS (SULFONIC ACID)
200C 20 Mr 16-50 Na 48 150 1.7 4.2 0-14
IR120 H 8 G 16-50 H 45 121 1.9 4.4 0-14
IR122 Na 10 G 16-50 Na 40 121 2.1 4.4 0-14
15 2 — Mr 16-50 H <1 120 1.8 4.7 0-14
1200 H 3 — G 650µm H 52 120 1.8 4.7 0-14
IRN77 8 G 16-50 H ≤55 121 1.8 5.0 0-14
DIAION STRONG CATION EXCHANGERS (SULFONIC ACID)
EXC04 — HP 16-50 H 55 120 1.2 — 0-14
HPK25 — HP 16-50 Na 42 125 1.8 3.9 0-14
PK208 4 P 16-50 Na 63 120 1.2 4.3 0-14
PK212 6 P 16-50 Na 55 120 1.5 — 0-14
PK216 8 P 16-50 Na 49 120 1.75 — 0-14
PK220L 10 P 16-40 Na 44 120 1.9 — 0-14

O r d e r : 1 . 8 0 0 . 3 2 5 . 3 0 1 0 Te c h n i c a l S e r v i c e : 1 . 8 0 0 . 3 5 9 . 3 0 4 1 We b : w w w. s i g m a - a l d r i c h . c o m / s u p e l c o
PK228L 14 P 16-50 Na 40 120 2.0 4.2 0-14
RCP160M — HP 25-60 H 50 120 1.5 4.5 0-14
SK1B 8 G 16-50 Na 46 120 1.9 3.6 0-14
SK1BS 8 G 150-350µm Na 46 120 1.9 4.0 0-14
SK104 4 G 16-50 Na 62 120 1.2 4.0 0-14
SK110 10 G 16-50 Na 40 120 2.0 — 0-14
SK112 12 G 16-50 Na 37 120 2.1 3.9 0-14
SK116 16 G 16-50 Na 32 120 2.1 3.6 0-14
UBK510L 4 G 300-360µm Na 40 120 1.9 — 0-14
UBK555 8 G 200-240µm Ca 44 120 2.0 — 0-14
DOWEX STRONG CATION EXCHANGERS (SULFONIC ACID)
50Wx2 2 G 50-100 H 78 150 0.6 4.8 0-14
50Wx2 2 G 100-200 H 78 150 0.6 4.8 0-14
50Wx2 2 G 200-400 H 78 150 0.6 4.8 0-14
50Wx4 4 G 50-100 H 68 150 1.1 4.8 0-14
50Wx4 4 G 100-200 H 68 150 1.1 4.8 0-14
50Wx4 4 G 200-400 H 68 150 1.1 4.8 0-14
50Wx8 8 G 50-100 H 53 150 1.7 4.8 0-14
50Wx8 8 G 100-200 H 54 150 1.7 4.8 0-14
50Wx8 8 G 200-400 H 54 150 1.7 4.8 0-14

Resins & Media

DR-2030 — Mp 30-60 H 3 150 1.7 4.7 0-14
HCR-W2 8 G 16-40 H 52 150 1.8 4.8 0-14
HCR-W2 8 G 16-40 Na 46 150 2.0 3.7 0-14
HGR-W2 10 G 16-40 H 40 150 2.2 4.2 0-14
MSC4 — Mp 520µm H 47 150 1.7 4.5 0-14
MSC4 — Mp 550µm Na 47 150 1.7 4.5 0-14
650C 5 — G 650µm H 48 150 1.9 4.6 0-14
650C UPW5 — G 650µm H 48 150 1.9 4.6 0-14
G-26 — G 650µm H 48 150 1.9 4.6 0-14
88 — Mp 16-40 Na 45 43 1.8 3.8 0-14
88 5 — Mp 550µm Na 45 43 1.8 3.8 0-14
M-31 — Mp 16-40 H 52 150 1.7 4.7 0-14
M-31 5 — Mp 475µm H 52 150 1.7 4.7 0-14
99K/320 5 — G 320µm K 59 150 1.5 4.5 0-14
99K/350 5 — G 350µm K 59 150 1.5 4.5 0-14
99Ca/320 5 6% G 320µm Ca 59 150 1.5 4.5 0-14
99Ca/350 5 — G 350µm Ca 59 150 1.5 4.5 0-14
Marathon C 4 8% G 550µm H 53 150 1.8 5.6 0-14
N-406 — G 650µm H 49 150 1.9 — 0-14

1
G = gel; Mr = macroreticular; Mp = macroporous; HP = highly porous; P = porous
2
Amberlyst exchanger
3
Amberjet exchanger
4
Marathon exchanger
5
Monosphere exchanger

SUPELCO
 450
Resins
Cation Exchange Media

Comparable Strongly Acidic Diaion Strong Cation Exchangers

Cation Exchangers on Polystyrene Functional Group: sulfonic acid
AMBERLITE DIAION DOWEX Effective pH Range:0-14
200/252 1 PK228/PK216 Meet requirements of FDA Food Additive
Regulation 21 CFR 173.25.
IR-118H5, 31 1 QTY. CAT. NO. PRICE
1
IR-120 (H) SK1B HCR-S , HCR-W2 PK228L
IR-122 SK110 HGR-W2 Polystyrene, porous Crosslinkage: 14% Form: sodium
SK104 50Wx4 Applications: decolorization; softening and deionization of water; recovery
and separation of metals; refining of chemicals, sugar and dextrose;
IR-1241 SK112L 50Wx121 catalyst; separation of amino acids (good stability against organic fouling
SK116 50Wx161 and oxidation)
Amberjet 1200 G-26, 650C 1000g 13563
N-437 1, N-406 SK116
1
Polystyrene, gel Crosslinkage: 16% Form: sodium
Not available. Applications: pharmaceutical separations; size exclusion (good stability
against oxidation)
100g 13581
Amberlite Strong Cation Exchangers UBK555
Polystyrene, gel Crosslinkage: 8% Form: calcium Particle Size: 200-
Functional Group: sulfonic acid 240µm Applications: fructose/glucose chromatographic separation
1000g 13968-U
O r d e r : 1 . 8 0 0 . 3 2 5 . 3 0 1 0 Te c h n i c a l S e r v i c e : 1 . 8 0 0 . 3 5 9 . 3 0 4 1 We b : w w w. s i g m a - a l d r i c h . c o m / s u p e l c o

Effective pH Range:0-14
QTY. CAT. NO. PRICE
Amberlite 200
Polystyrene, macroreticular Crosslinkage: 20% Form: sodium Applica- Dowex Strong Cation Exchangers
tions: water conditioning; removal of heavy metals; decalcification of blood
and extracorporeal transfusion (superior resistance to oxidation). Functional Group: sulfonic acid
Meets requirements of FDA Food Additive Regulation 21 CFR 173.25. Effective pH Range:0-14
1000g 06458-1KG
Meet requirements of FDA Food Additive Regulation 21 CFR 173.25
IR120 (unless otherwise noted).
Polystyrene, gel Crosslinkage: 8% Form: hydrogen Applications: wide
variety of chemical process applications; removal of amino acids at low pH; QTY. CAT. NO. PRICE
USP potassium methods. 50WX2
Meets requirements of FDA Food Additive Regulation 21 CFR 173.25.
500g 10322 Polystyrene, gel Crosslinkage: 2% Form: hydrogen
Amberlyst 15 (dry) Mesh Size: 50-100
Polystyrene, macroreticular Form: hydrogen Swelling (dry to solvent 100g 217441-100G
saturated, %) hexane: 10-15 toluene: 10-15 ethylene dichloride: 15-20 500g 217441-500G
ethylene acetate: 30-40 ethylene alcohol: (95%) 60-70 water: 60-70 2500g 217441-2.5KG
Applications: heterogeneous acid catalysis 1 cubic foot 217441-1FT3
100g 10389 Mesh Size: 100-200
100g 217468-100G
Amberlite IRN77 500g 217468-500G
Polystyrene, gel, Nuclear Grade Form: hydrogen , minimum of 99% 2500g 217468-2.5KG
exchange sites Applications: water treatment, RAD waste treatment; 1 cubic foot 217468-1FT3
Resins & Media

decontamination Mesh Size: 200-400

500g 10342 100g 217476-100G
500g 217476-500G
2500g 217476-2.5KG
1 cubic foot 217476-1FT3
HELPFUL HINTS 50WX4
Numerous Dow, Misubishi, and Rohm & Haas resins are available Polystyrene, gel Crosslinkage: 4% Form: hydrogen
in the Supelco warehouse but not listed here. If a resin you need Mesh100g
Size: 50-100
428663-100G
is not listed here, please contact your local Sigma-Aldrich 500g 428663-500G
representative with your requirements. 2500g 428663-2.5KG
1 cubic foot 428663-1FT3
Mesh Size: 100-200
100g 422096-100G
500g 422096-500G
2500g 422096-2.5KG
1 cubic foot 422096-1FT3
Mesh Size: 200-400
100g 217484-100G
500g 217484-500G
2500g 217484-2.5KG
1 cubic foot 217484-1FT3

SUPELCO
 451
Resins
Cation Exchange Media

Dowex Strong Cation Exchangers (cont’d) Dowex Strong Cation Exchangers (cont’d)
QTY. CAT. NO. PRICE QTY. CAT. NO. PRICE
50WX8 88
Polystyrene, macroporous Particle Size: 16-40 mesh Form: sodium
Polystyrene, gel Crosslinkage: 8% Form: hydrogen Applications: deashing and mixed bed polishing of high fructose corn
Mesh Size: 50-100 syrups
100g 217492-100G 1000g 436682-1KG
500g 217492-500G 2500g 436682-2.5KG
2500g 217492-2.5KG
1 cubic foot 217492-1FT3 Monosphere 88
Mesh Size: 100-200 Polystyrene, macroporous, monodispersed Particle Size: 550µm Form:
100g 217506-100G sodium Applications: same as for Dowex 88.
500g 217506-500G 1000g 13709
2500g 217506-2.5KG M-31
1 cubic foot 217506-1FT3 Polystyrene, macroporous Form: hydrogen Particle Size: 16-40 mesh
Mesh Size: 200-400 Applications: catalyst
100g 217514-100G Does not meet FDA Food Additive Regulation 21 CFR 173.25
500g 217514-500G 100g 573671-100G
2500g 217514-2.5KG 500g 573671-500G
1 cubic foot 217514-1FT3 5000g 573671-5KG
DR-2030 (dry, made from Dowex M-31) 1 cubic foot 573671-1FT3
Polystyrene, macroporous Form: hydrogen Applications: catalyst Monosphere M-31
Does not meet FDA Food Additive Regulation 21 CFR 173.25 Polystyrene, macroporous, monodispersed Particle Size: 475µm

O r d e r : 1 . 8 0 0 . 3 2 5 . 3 0 1 0 Te c h n i c a l S e r v i c e : 1 . 8 0 0 . 3 5 9 . 3 0 4 1 We b : w w w. s i g m a - a l d r i c h . c o m / s u p e l c o
100g 446483-100G Form: hydrogen Particle Size: 475µm Applications: same as for Dowex
500g 446483-500G M-31
5000g 446483-5KG Does not meet FDA Food Additive Regulation 21 CFR 173.25
1 cubic foot 446483-1FT3 1000g 13697
HCR-W2 Monosphere 99K/320
Polystyrene, gel Crosslinkage: 8% Form: hydrogen Polystyrene, gel, monodispersed Particle Size: 320µm Form: potassium
1000g I 8880-1KG Applications: chromatographic separations; sucrose recovery from beet
HCR-W2 and cane molasses (molasses desugarization or ion exclusion); separation of
Polystyrene, gel Crosslinkage: 8% Form: sodium Applications: mixed-bed salts from polar organics; bulk deashing of process streams via ion exclusion
applications; condensate polishing; demineralization; organic solvent chromatography; size exclusion separations of mono-, di-, and trisaccha-
desiccants; water softening (sodium form has high capacity for most rides and other polar organics; betaine purification/recovery; basic amino
organic liquids) acid recovery from sugar syrups and fractionation of basic amino acids;
1000g 13443 refining of polysaccharides to eliminate monosaccharides and low DP
oligosaccharides
Marathon C 1000g 13713
Polystyrene, gel. monodispersed Particle Size: 550µm Form: hydrogen
Applications: demineralization Monosphere 99K/350
1000g 433950-1KG Polystyrene, gel, monodispersed Particle Size: 350µm Form: potassium
Applications: same as for Monosphere 99K/320, but for high flow rate or
Marathon MSC (previously known as MSC-1) deep bed applications where minimizing pressure drop is desired
Polystyrene, macroporous, monodispersed Particle Size: 520µm Form: 1000g 13717
hydrogen Applications: Water demineralization
2500g MSC1-2.5KG Monosphere 99Ca/320
Polystyrene, gel, monodispersed Particle Size: 320µm Form: calcium
Monosphere 650C Applications: chromatographic separation of sugars, including specialty
Polystyrene, gel Particle Size: 650µm Form: hydrogen Applications: sugars and sugar alcohols; major industrial use is for separation of fructose

Resins & Media

mixed-bed applications; condensate polishing; sugar applications from 42% high fructose corn syrup
(Monosphere 650C material) 1000g 13721
1000g 13471-U
Monosphere 650C UPW (purified 650C)
Polystyrene, gel. Form: hydrogen Applications: demineralization for
ultrapure water HELPFUL HINTS
1000g 13340-U
Numerous Dow, Misubishi, and Rohm & Haas resins are available
G-26 ( same material as 650C, but mining grade)
Polystyrene, gel Form: hydrogen Applications: chemical processing; in the Supelco warehouse but not listed here. If a resin you need
mining. is not listed here, please contact your local Sigma-Aldrich
Does not meet FDA Food Additive Regulation 21 CFR 173.25 representative with your requirements.
100g 573663-100G
500g 573663-500G
5000g 573663-5KG
1 cubic foot 573663-1FT3

SUPELCO
 452
Resins
Cation Exchange Media

Weak Cation Exchangers on Polyacrylic Copolymer

CROSS MAX. OP. TOTAL
LINK IONIC MOISTURE TEMP. EXCHANGE CAPACITY pH
EXCHANGER (%) MATRIX1 MESH FORM (Approx. %) (°C) 2 meq/mL meq/g RANGE
AMBERLITE WEAK CATION EXCHANGERS (CARBOXYLIC ACID)
CG-50 Type I 4 Mr 100-200 H 10 120 3.5 10.0 5-14
IRC50 4 Mr 16-50 H 48 100 3.5 10.0 5-14
IRC76 — Mr 16-50 H 56 120 4.0 11.0 5-14
IRC86 — G 16-50 H 50 120 4.2 10.7 4-14
DIAION WEAK CATION EXCHANGERS (CARBOXYLIC ACID)
CWK30/S — P 30-60 H 49 120 4.5 — 4-14
WK10 — P 16-50 H 56 150 2.5 — 5-14
WK11 — P 16-50 H 48 150 2.9 — 5-14
WK40 — HP 16-50 H 46 200 4.4 10.2 4-14
WK100 — HP 16-50 H 49 120 2.8 9.0 4-14
WT01S — HP 100 -200µm H 50 120 3.0 9.0 4-14
DOWEX WEAK CATION EXCHANGER (CARBOXYLIC ACID)
MAC-3 — Mp 16-50 H 47 120 3.8 10.8 4-14
O r d e r : 1 . 8 0 0 . 3 2 5 . 3 0 1 0 Te c h n i c a l S e r v i c e : 1 . 8 0 0 . 3 5 9 . 3 0 4 1 We b : w w w. s i g m a - a l d r i c h . c o m / s u p e l c o

Comparable Weakly Acidic Cation Exchangers on

Polyacrylic Copolymer
AMBERLITE DIAION DOWEX
CG-50 Type I WT01S
DP-15, IRC-50 WK100
IRC-76, IRC-845 WK205, WK40 CCR-25, MWC-1 5
IRC-845, IRC-86 WK205, WK40 CCR-35, MAC-3
1
G = gel; Mr = macroreticular; Mp = macroporous; HP = highly porous; P = porous
2
Amberlyst exchanger
3
Amberjet exchanger
4
Marathon exchanger
5
Not available.

Amberlite Weak Cation Exchangers Diaion Weak Cation Exchangers

Functional Group: carboxylic acid Functional Group: carboxylic acid
Meets requirements of FDA Food Additive Regulation 21FT3R
Meet requirements of FDA Food Additive
Regulation 21 CFR 173.25
173.25
Resins & Media

QTY. CAT. NO. PRICE

QTY. CAT. NO. PRICE
CG-50 Type I (dry, fine mesh IRC-50)
WT01S
Acrylic, macroreticular Form: hydrogen Particle Size: 75-100µm Acrylic, highly porous Form: hydrogen Particle Size: 100-200µm
Applications: cytochrome c isolation and purification; amines; drugs; metal Applications: metal recovery; dealkalization; iron removal; refining of
ions; neutralization of solutions; thrombin sugar; purification of antibiotics, pharmaceuticals, amino acids, etc.
500g 10319 (superior kinetics and mechanical strength)
IRC50 100g 13593-U
Acrylic, macroreticular Form: hydrogen Particle Size: 300-1200µm 1000g 13595-U
Applications: selectively adsorbs organic bases such as antibiotics,
alkaloids, peptides, and amino acids; many proteins including higher
molecular weights; neutralization of reaction mixtures; good all around use Dowex Weak Cation Exchanger
500g 10338
Functional Group: carboxylic acid
IRC76
Acrylic, macroreticular Form: hydrogen Applications: removal of ions QTY. CAT. NO. PRICE
associated with carbonate and bicarbonate alkalinity; deionization;
softening MAC-3
500g 10340-U Acrylic, macroporous Form: hydrogen Applications: water treatment;
dealkalization; purification of antibiotics, pharmaceuticals, amino acids, etc.
1000g 502545D

HELPFUL HINTS
Numerous Dow, Misubishi, and Rohm & Haas resins are available
in the Supelco warehouse but not listed here. If a resin you need
is not listed here, please contact your local Sigma-Aldrich
representative with your requirements.

SUPELCO
 453
Resins
Ion Exchange (Chelating) Media

Chelating Resins
CROSS MAX. OP. TOTAL
LINK IONIC MOISTURE TEMP. EXCHANGE CAPACITY pH
EXCHANGER (%) MATRIX1 MESH FORM (Approx. %) (°C) 2 meq/mL meq/g RANGE
AMBERLITECHELATING RESIN
IRC-748 — Mr 16-50 Na 65 90/70 2 1.25 4.4 1.5-14
IRA743 — Mr 16-50 FB ≤58 100 0.6 — 0-10
DIAION CHELATING RESINS
CR11 — HP 16-50 Na 60 120/802 0.35 (Ca) 1.2 (Ca) 4-10
CR20 — HP 16-50 FB 55 100 0.28 (Ca) 0.91 (Ca) 6-10
CRB02 — HP 16-50 amine 55 — 0.6 — —
DOWEX CHELATING RESINS
M4195 — Mp 16-50 SO 4 62 60 35g Cu +2/L — 0-7
DUOLITE CHELATING RESINS
C467 — Mp 16-50 Na 62 65 1.0 3.5 1-10
GT73 — Mp 16-50 H 55 121 1.2 3.9 1-13
1
Mp = macroporous Mr = macroreticular HP = highly porous

O r d e r : 1 . 8 0 0 . 3 2 5 . 3 0 1 0 Te c h n i c a l S e r v i c e : 1 . 8 0 0 . 3 5 9 . 3 0 4 1 We b : w w w. s i g m a - a l d r i c h . c o m / s u p e l c o
2
Na/H

Chelating Resins
QTY. CAT. NO. PRICE HELPFUL HINTS
Amberlite IRC-748 Numerous Dow, Misubishi, and Rohm & Haas resins are available
Macroreticular Functional Group: iminodiacetic acid Form: sodium
Applications: high affinity for heavy metal cations over alkali or alkaline in the Supelco warehouse but not listed here. If a resin you need
earth metals; ideal for use in nonaqueous media is not listed here, please contact your local Sigma-Aldrich
100g 13296-U representative with your requirements.
1000g 13297-U
2500g 13298-U
5000g 13299-U
Amberlite IRA743
Macroreticular Form: free baseApplications: Removal of borate, boric
acid, other boron species from water, highly selective; salts, including bases,
do not interfere significantly
25g IRA743-25G
100g IRA743-100G
500g IRA743-500G
Diaion CR11
Highly porous Functional Group: iminodiacetic acid

Resins & Media

Form: sodium Applications: metal recovery; wastewater treatment; brine
purification
1000g 13547
Diaion CRB02
Highly porous Functional Group: glucamine Form: free amine Applica-
tions: high selectivity for borate ion; best for low levels,
high flow
1000g 13959-U
Dowex M4195
Macroporous Functional Group: bis-picolylamine Applications: removal of
cobalt, copper, nickel; metal recovery; mining; general hydrometallurgy
100g 13727-U
500g 13728-U
1000g 13729-U
Duolite C467
Macroporous Functional Group: amino-phosphonic Form: sodium
Applications: metallic cations of low mass; removal of hardness from brine;
catalyst (resistant to osmotic shock)
500g 10353
Duolite GT73
Macroporous Functional Group: thiol Form: hydrogen
Applications: removal of mercury, silver, lead, copper, cadmium
500g 10354

SUPELCO
 454
Resins
Ion Exchange (Mixed Bed) Media

Mixed Bed Resins on Polystyrene

CROSS MAX. OP. TOTAL
LINK IONIC MOISTURE TEMP. EXCHANGE CAPACITY pH
EXCHANGER (%) MATRIX1 MESH FORM (Approx. %) (°C) 2 meq/mL meq/g RANGE
AMBERLITE MIXED BED RESINS
MB-150 — G 16-50 H, OH 60 60 0.55 2.0 0-14
IRN150 8 G 16-50 H, OH ≤60 60 0.55 2.0 0-14
DIAION MIXED BED RESINS
SMNUP — G 16-50 H, OH 60 60 — — 0-14
SMT100 — G 16-50 H, OH 60 60 — — 0-14
DOWEX MIXED BED RESINS
MR-3 LC NG — G 16-50 H, OH 60 60 1.7 — 0.14
Marathon MR-3 — G 550-660µm H, OH 60 60 1.4 — 0-14
MR-3 UPW4 — G 600µm H, OH 60 60 1.0 — 0-14
MR-450 UPW4 — G 360,
590µm H, OH 53 60 1.0 — 0-14
11A8 Retardion — G 35-80 NA2 45 70 NA 2 NA2 0-14
OTHER RESINS
O r d e r : 1 . 8 0 0 . 3 2 5 . 3 0 1 0 Te c h n i c a l S e r v i c e : 1 . 8 0 0 . 3 5 9 . 3 0 4 1 We b : w w w. s i g m a - a l d r i c h . c o m / s u p e l c o

TMD-8 — G 16-40 H, OH — 41 0.55 3 0.80 3 0-14

Comparable Mixed Bed Resins on Polystyrene

AMBERLITE DIAION DOWEX OTHER
MB-1 5, MB-150 SMNUP MR-3 —
MB-3 5 — — TMD-8
IRN150 SMNUP MR-3 —
1
G = gel
2
Not applicable
3
Indicating; color changes (blue to amber) when capacity is reached.
4
Monosphere resin
5
Not available.

Mixed Bed Resins

QTY. CAT. NO. PRICE QTY. CAT. NO. PRICE
Amberlite MB-150 Marathon MR-3
Contains Amberlite 440 (OH) and IR-120 (H) resins. Form: hydrogen and hydroxide Applications: specially processed resin for
Applications: deionization. mixed bed condensate polishing; useful for in-process demineralization.
100g 13679 100g 13686-U
Resins & Media

1000g 13681 1000g 13687-U

Amberlite IRN150 Monosphere MR-450 UPW
Nuclear Grade Form: hydrogen and hydroxide, minimum of 99% and 95% Particle Size: 360µm, 590µm Applications: ultrapure water
exchange sites, respectivelyApplications: primary water chemistry control 1000g 13349-U
in once-through systems; useful in industrial water treatment where as- Dowex 11A8 Retardation
supplied resin must have absolute minimum of ionic and non-ionic Form: contains paired anion and cation exchange sites Applications:
contamination. removal of SDS, other ionic detergents from protein samples; desalting by
500g 10341 SEC-type mechanism.
Diaion SMNUP (Mitsubishi) 100g I 6878-100G
Nuclear Grade, contains Diaion SANUP (OH) and Diaion SKNUP (H) resins 500g I 6878-500G
Applications: deionization; nuclear grade mixed bed for high quality water TMD-8
purification A mixture of strong cation and anion resins Indicating resin: color changes
100g 13901 from blue to amber when capacity is reached
1000g 13903-U 25g M8157-25G
MR-3 LC NG 100g M8157-100G
Nuclear Grade Form: hydrogen and hydroxide, minimum of 99.7% and 500g M8157-500G
95% exchange sites, respectivelyApplications: low chloride, mixed bed 1000g M8157-1KG
resin for high quality water production in nuclear and industrial applications
100g 13683-U
1000g 13684-U

HELPFUL HINTS
Numerous Dow, Misubishi, and Rohm & Haas resins are available
in the Supelco warehouse but not listed here. If a resin you need
is not listed here, please contact your local Sigma-Aldrich
representative with your requirements.

SUPELCO
 455
Resins
Ion Exchange (Nuclear) Media

Nuclear Resins
CROSS MAX. OP. TOTAL
LINK IONIC MOISTURE TEMP. EXCHANGE CAPACITY pH
EXCHANGER (%) MATRIX1 MESH FORM (Approx. %) (°C) 2 meq/mL meq/g RANGE
AMBERLITENUCLEAR RESINS
IRN-77 — G 16-50 H2 <55 121 1.8 5.0 0-14
IRN-78 — G 16-50 OH3 <60 60 1.1 4.0 0-14
IRN-150 — G 16-50 H, OH <60 60 0.55 2.0 0-14
DIAION NUCLEAR RESINS
SMNUP — G 16-50 H, OH 60 60 0.7 — 0-14
DOWEX NUCLEAR RESINS
SBR LC NG 8 G 16-50 OH ≤60 60 1.1 — 0-14
MR-3 LC NG — G 16-50 H, OH ≤60 60 1.7 — 0-14

Comparable Nuclear Resins

AMBERLITE DIAION DOWEX
IRN-77 SAN 15 SBR LC NG

O r d e r : 1 . 8 0 0 . 3 2 5 . 3 0 1 0 Te c h n i c a l S e r v i c e : 1 . 8 0 0 . 3 5 9 . 3 0 4 1 We b : w w w. s i g m a - a l d r i c h . c o m / s u p e l c o
IRN-78 SKN 15
IRN-150 SMN 15, SMNUP MR-3 LC NG
1
G = gel
2
Minimum of 99% of exchange sites
3
Minimum of 95% of exchange sites
4
High porosity
5
Not available from Supelco

Nuclear Resins
QTY. CAT. NO. PRICE HELPFUL HINTS
Amberlite IRN77 Numerous Dow, Misubishi, and Rohm & Haas resins are available
Polystyrene, gel, Nuclear Grade Form: hydrogen , minimum of 99%
exchange sites Applications: water treatment, RAD waste treatment; in the Supelco warehouse but not listed here. If a resin you need
decontamination is not listed here, please contact your local Sigma-Aldrich
500g 10342 representative with your requirements.
IRN78
Polystyrene, gel, Nuclear Grade Form: hydroxide, minimum of 95%
exchange sites Applications: Water treatment, RAD waste, decontamina-

Resins & Media

tion, useful for boron thermal regeneration.
500g 10343
Amberlite IRN150
Nuclear Grade Form: hydrogen and hydroxide, minimum of 99% and 95%
exchange sites, respectivelyApplications: primary water chemistry control
in once-through systems; useful in industrial water treatment where as-
supplied resin must have absolute minimum of ionic and non-ionic
contamination.
500g 10341
Diaion SMNUP (Mitsubishi)
Nuclear grade, contains Diaion SANUP (OH) and Diaion SKNUP (H) resins
Applications: deionization; nuclear grade mixed bed for high quality water
purification
100g 13901
1000g 13903-U
SBR LC NG
Polystyrene, gel, Nuclear Grade Form: hydroxide, minimum of 95%
exchange sites Applications: Water treatment, CVCS and radwaste
demineralizers, reactor coolant treatment; useful for boric acid recovery
100g 14035-U
1000g 14036-U
2500g 14037-U
5000g 14038-U
MR-3 LC NG
Nuclear grade Form: hydrogen and hydroxide, minimum of 99.7% and
95% exchange sites, respectivelyApplications: low chloride, mixed bed
resin for high quality water production in nuclear and industrial applications
100g 13683-U
1000g 13684-U

SUPELCO
 456
Resins
Ion Exchange Media

Ion Exchange Media (Toyopearl)

Toyopearl ion exchange resins feature covalently bonded diethylaminoethyl (DEAE), carboxymethyl (CM), sulfopropyl (SP), or
trimethylammonium (QAE and Q) groups attached to large pore size methacrylate particles. These materials ensure highates
recovery
for a r
wide variety of proteins. In addition, they offer several other advantages:
● Sample capacity up to 2-4 times that of conventional gels
● High mechanical strength – you can use high flow rates
● Isocratic or gradient elution (bed volume will not change)
● Easily sanitized with acid, base, or heat

Toyopearl Resins for Ion Exchange Chromatography (all 250mL)

PARTICLE EXCHANGE ADSORPTION
TOYOPEARL SIZE EXCHANGER TYPE CAPACITY CAPACITY
RESIN (µm) (OPERATING pH) (meq/mL GEL) (mg/mL GEL) CA T. NO. PRICE
DEAE-650C 60-150 weak anion (2-10) 0.10 ±0.02 30 ±5 1 807988
DEAE-650M 40-90 0.10 ±0.02 30 ±5 1 807473
DEAE-650S 20-50 0.10 ±0.02 30 ±51 807472
Super Q-650M 40-90 strong anion (2-12) 0.20-0.30 143 1 817227
Super Q-650S 20-50 0.20-0.30 143 1 817223
O r d e r : 1 . 8 0 0 . 3 2 5 . 3 0 1 0 Te c h n i c a l S e r v i c e : 1 . 8 0 0 . 3 5 9 . 3 0 4 1 We b : w w w. s i g m a - a l d r i c h . c o m / s u p e l c o

QAE-550C 60-150 strong anion (2-10) 0.37 70 1 814026

CM-650M 40-90 weak cation 0.10 ±0.02 50 ±10 2 807475
SP-650C 60-150 strong cation (3-11) 0.15 ±0.02 55 ±10 3 807994
SP-650M 40-90 0.15 ±0.02 55 ±10 3 807997
SP-550C 60-150 0.15 1111 814028

LABPAK Ion Exchange Resin Kits 4

LABPAK samplers enable you to try several Toyopearl resins to determine which works best for your particular application.
kit contains
Each
several resins, grouped by separation mechanism.
PARTICLE SIZE EXCHANGER
KIT RESIN (µm) TYPE CAT. NO. PRICE
AIEXPAK Sampler
100mL each resin 843210
DEAE-650M 40-90 weak anion
QAE-550C 50-150 strong anion
Super Q-650M 40-90 strong anion
Resins & Media

1
Adsorption capacity measured with: bovine serum albumin
2
Adsorption capacity measured with: bovine hemoglobin
3
Adsorption capacity measured with: lysozyme
4
Exclusion limits (globular proteins): 550 type resins: 700,000 Dalton; 650 type resins: 5,000,000 Dalton.

HELPFUL HINTS
Numerous Dow, Misubishi, and Rohm & Haas resins are available
in the Supelco warehouse but not listed here. If a resin you need
is not listed here, please contact your local Sigma-Aldrich
representative with your requirements.

SUPELCO