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BLAST Info For Introduction With Citation

After performing PCR to obtain DNA sequences, BLAST can be used to compare sequences to databases and determine matches. BLAST is maintained by NCBI and compares queries to find the best statistical alignment. BlastN compares nucleotide sequences to nucleotide databases, while BlastX can find the most likely protein sequence from a nucleotide query. BLAST results can help identify unknown sequences or their location in genes.

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Charli Ann Camden
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65 views1 page

BLAST Info For Introduction With Citation

After performing PCR to obtain DNA sequences, BLAST can be used to compare sequences to databases and determine matches. BLAST is maintained by NCBI and compares queries to find the best statistical alignment. BlastN compares nucleotide sequences to nucleotide databases, while BlastX can find the most likely protein sequence from a nucleotide query. BLAST results can help identify unknown sequences or their location in genes.

Uploaded by

Charli Ann Camden
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
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After performing PCR and successfully obtaining product, a Basic Local Alignment Search Tool (BLAST)

can be used to determine various aspects of the DNA sequence. BLAST is a computer program
maintained by the National Center for Biotechnology Information (NCBI). Its purpose is to scan a
sequence of nucleotides or amino acids and compare it to all available sequence databases for the best
match (*). The two BLAST search options we utilized were BlastN and BlastX. BlastN compares a
nucleotide query sequence against a nucleotide sequence database to statistically determine the most
accurate alignment. A nucleotide query sequence can also be entered in BlastX for the most likely
protein sequence. The results of these searches can aid in determining the source of an unknown
sequence or in determining its possible location within the gene.

(*) Citation Source:

Altschul, SF, Gish, W, Miller, W, Myers, EW, and DJ Lipman (1990). Basic local alignment search tool.
J.  Mol.  Biol. 215:403–10.

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